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1 RETURN BIDS TO: RETOURNER LES SOUMISSIONS À: Bid Receiving - PWGSC / Réception des soumissions - TPSGC 11 Laurier St. / 11, rue Laurier Place du Portage, Phase III Core 0A1 / Noyau 0A1 Gatineau, Québec K1A 0S5 Bid Fax: (819) Revision to a Request for a Standing Offer Révision à une demande d offre à commandes National Individual Standing Offer (NISO) Offre à commandes individuelle et nationale (OCIN) The referenced document is hereby revised; unless otherwise indicated all other terms and conditions of the Offer remain the same. Ce document est par la présente révisé; sauf indication contraire, les modalités de l`offre demeurent les mêmes. Comments - Commentaires Vendor/Firm Name and Address Raison sociale et adresse du fournisseur/de l'entrepreneur Title-Sujet Chemical Residue Testing of Food Products Solicitation No. - N o de l invitation /B Client Reference No. - N o de référence du client Date Amendment no. No modif. 002 GETS Reference No. - N o de référence de SEAG File No. N o de dossier CCC No./N o CC FMS NO. / N o VME 052ss Date of Original Request for Standing Offer Date de la demande de l offre à commandes originale Solicitation Closes L invitation prend fin Time Zone Fuseau horaire at à 2:00 PM Eastern Standard on le Time EST Delivery Required - Livraison exigée See Herein Address Enquiries to: - Adresser toutes questions à: Brooke Taylor Telephone No. - N de téléphone Buyer Id Id de l acheteur 052ss Destination of Goods, Services and Construction: Destinations des biens, services et construction : Address: Specified Herein Précisé dans les présentes Security Sécurité This revision does not change the security requirements of the Offer. Cette révision ne change pas les besoins en matière de sécurité de la présente offre. Instructions : See Herein Instructions : voir aux présentes Issuing Office - Bureau de distribution Science Procurement Directorate/ Direction de l'acquisition de travaux scientifiques 11C1, Phase III Place du Portage 11 Laurier St. / 11, rue Laurier Gatineau, Québec K1A 0S5 The Offeror hereby acknowledges this revision to its Offer. Le proposant constate, par la présente, cette révision à son offre. Signature Date Name and title of person authorized to sign on behalf of Offeror. (type or print) Nom et titre de la personne autorisée à signer au nom du proposant. (taper ou écrire en caractères d'imprimerie) For the Minister Pour le Ministre

2 TITLE: MULTIPLE NATIONAL INDIVIDUAL STANDING OFFERS FOR CHEMICAL RESIDUE TESTING IN AND ON FOOD PRODUCTS FOR THE CANADIAN FOOD INSPECTION AGENCY The purpose of this amendment is to amend the solicitation document as detailed below, and to amend Attachment A - Questions and Answers to respond to additional questions received by offerors. 1. AT PART 4 - EVALUATION PROCEDURES AND BASIS OF SELECTION, MANDATORY TECHNICAL CRITERIA, TABLE 1, PAGE 18 OF 124: ARSENIC SPEC The SOP must provide for a protease digestion for all samples other than juices. The SOP must include a control sample or certified reference material for each batch analysed. The resolution of Standard 2 peaks of AsC and AsB as per the reference method (0.001 ng/ml AsC; ng/ml AsB) must have a resolution of 0.9 or greater. ARSENIC SPEC The SOP must provide for a protease digestion for all samples other than juices. The SOP must include a control sample or certified reference material for each batch analysed. The resolution of Standard 2 peaks of AsC and AsB as per the reference method (1 ng/ml AsC; 0.5 ng/ml AsB) must have a resolution of 0.9 or greater. Page 2 of 5

3 2. AT REQUIREMENT, ANNEX A, APPENDIX 1 TO ANNEX A, CHEMICAL RESIDUES OF INTEREST TO CFIA PAGE 69 OF 124: MACROLIDE S / LINCOSAMID ES CFIA Saskatoon CVDR Sample is made basic and extracted with ethyl acetate. Analytes are then partitioned into an acidic buffer and further cleaned up by extraction of the buffer solution with an organic solvent. The buffer is then made basic and analytes are re-extracted into ethyl acetate, evaporated to dryness, redissolved in mobile phase, and analyzed by HPLC/MS. Dairy Egg muscle) muscle); Optional for Dairy, Egg, Clindamycin Erythromycin Josamycin Lincomycin Oleandomycin Pirlimycin Spiramycin Tylosin tilmicosin. Desmycosin Neospiramycin CP-60,300 expressed as Tulathromycin equivalents Gamithromycin Tildipirosin Tylvalosin Optional for Dairy, Egg, honey Confirmation using an acceptable MS technique is required. See Tasks/ Specification s The ANALYTE is to be reported as Macrolides/Lincosa mide Screen and the AMOUNT is to be 0 for a negative and a 1 for a positive for one or more of the analytes. In the event of a positive, the analyte(s) found to be positive is/are to be reported as a separate entry and the amount as the actual value confirmed, in µg/g. MACROLIDE S / LINCOSAMID ES CFIA Saskatoon CVDR Sample is made basic and extracted with ethyl acetate. Analytes are then partitioned into an acidic buffer and further cleaned up by extraction of the buffer solution with an organic solvent. The buffer is then made basic and analytes are re-extracted into ethyl acetate, evaporated to dryness, redissolved in mobile phase, and analyzed by HPLC/MS. Note: All samples found positive above the LOQ for tulathromycin are confirmed using a hydrolysis method for CP 60,300 and reported using the amount found of CP 60,300 as tulathromycin equivalents. Dairy Egg muscle) muscle); Optional for Dairy, Egg, Clindamycin Erythromycin Josamycin Lincomycin Oleandomycin Pirlimycin Spiramycin Tylosin tilmicosin. Desmycosin Neospiramycin CP-60,300 expressed as Tulathromycin equivalents Gamithromycin Tildipirosin Tylvalosin Optional for Dairy, Egg, honey Confirmation using an acceptable MS technique is required. See Tasks/ Specification s The ANALYTE is to be reported as Macrolides/Lincosa mide Screen and the AMOUNT is to be 0 for a negative and a 1 for a positive for one or more of the analytes. In the event of a positive, the analyte(s) found to be positive is/are to be reported as a separate entry and the amount as the actual value confirmed, in µg/g. Page 3 of 5

4 3. AT REQUIREMENT, ANNEX A, APPENDIX 1 TO ANNEX A, CHEMICAL RESIDUES OF INTEREST TO CFIA PAGE 83 OF 124: Arsenic Species CFIA Dartmouth SOM-CHE Samples are enzyme digested, extracted and analyzed on ICP-MS The SOP must provide for a protease digestion for all samples other than juices. The SOP must include a control sample or certified reference material for each batch analysed. The resolution of Standard 2 peaks of AsC and AsB as per the reference method (0.1 ng/ml AsC; 0.05 ng/ml AsB) must have a resolution of 0.9 or greater Egg, Fresh F&V Processed Foods Meat (muscle) Arsenocholine (AsC) See Tasks/ Arsenobetaine (AsB) Specifications Disodium methyl arsonate hexahydrate (MMA) Cacodylic acid (DMA) As 3+ As The ANALYTE is to be reported as Arsenic Speciation Screen and the AMOUNT is to be 0 for a negative and a 1 for a positive for one or more of the analytes. In the event of a positive, the analyte(s) found to be positive is/are to be reported as a separate entry and the amount as the actual value confirmed, in ng/g, as arsenic equivalents. Arsenic Species CFIA Dartmouth SOM-CHE Samples are enzyme digested, extracted and analyzed on ICP-MS The SOP must provide for a protease digestion for all samples other than juices. The SOP must include a control sample or certified reference material for each batch analysed. The resolution of Standard 2 peaks of AsC and AsB as per the reference method (1 ng/ml AsC; 0.5 ng/ml AsB) must have a resolution of 0.9 or greater Egg, Fresh F&V Processed Foods Meat (muscle) Arsenocholine (AsC) See Tasks/ Arsenobetaine (AsB) Specifications Disodium methyl arsonate hexahydrate (MMA) Cacodylic acid (DMA) As 3+ As The ANALYTE is to be reported as Arsenic Speciation Screen and the AMOUNT is to be 0 for a negative and a 1 for a positive for one or more of the analytes. In the event of a positive, the analyte(s) found to be positive is/are to be reported as a separate entry and the amount as the actual value confirmed, in ng/g, as arsenic equivalents. Page 4 of 5

5 4. AT ATTACHMENT A - QUESTIONS AND ANSWERS, INSERT THE FOLLOWING QUESTION AND ANSWER: Q6. With respect to Arsenic Species, Mandatory Criteria, Table 1 (pg 18 of 124) indicates that "the resolution of Standard 2 peaks of AsC and AsB as per the reference method (0.001 ng/ml AsC; ng/ml AsB) must have a resolution of 0.9 or greater". Appendix 1 to Annex A, Chemical Residues of Interest to CFIA (pg 84 of 124) indicates under the Mandatory Criteria of Arsenic Species that the resolution of Standard 2 peaks of AsC and AsB as per the reference method (0.1 ng/ml AsC; 0.05 ng/ml AsB) must have a resolution of 0.9 or greater. However, the Standard 2 concentrations of the two analytes AsC and AsB in the reference method (CFIA_ACIA # ), Table 2, page 18 are 1.0 ng/ml AsC and 0.5 ng/ml AsB. Could you please clarify what concentration is to be used? A6. The concentration to be used is 1 ng/ml AsC; 0.5 ng/ml AsB. ALL OTHER TERMS AND CONDITIONS REMAIN UNCHANGED Page 5 of 5

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