plaque reduction assay, modified dye uptake assay including formazan test, dye uptake assay
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1 Sauerbrei A, Bohn-Wippert K, Kaspar M, Krumbholz A, Karrasch M, Zell R Database on natural polymorphisms and resistance-related non-synonymous mutations in thymidine kinase and DNA polymerase genes of herpes simplex virus types 1 and 2. J Antimicrob Chemother in press, doi: /jac/dkv285, PMID: Supplementary Table 1: Natural polymorphisms in TK gene of HSV-1 as aa changes including nt changes, their location, antivirals tested, methods for phenotypic testing and references. Amino acid change Nucleotide change Domain Antivirals tested Test methods for phenotype PMID C6G A16G non-conserved region Aciclovir (ACV), Foscarnet (FOS) yield reduction assay (enyzme linked virus inhibitory reporter assay - ELVIRA) H7P A20C non-conserved region ACV, FOS modified dye uptake assay including formazan test A12P G34C non-conserved region ACV, FOS plaque reduction assay A12T G34A non-conserved region ACV, FOS modified dye uptake assay including formazan test D14V A41T non-conserved region ACV, Brivudin (BVDU), Penciclovir (PCV), Cidofovir (CDV), FOS D14Y G40T non-conserved region ACV, BVDU, PCV, CDV, FOS modified dye uptake assay including formazan test, dye uptake assay Q15L A44T non-conserved region ACV plaque reduction assay A17V C30T non-conserved region ACV dye uptake assay R18C C52T non-conserved region ACV, FOS modified dye uptake assay including formazan test S19P T55C non-conserved region ACV, BVDU, PCV, CDV, FOS R20H G59A non-conserved region ACV, FOS modified dye uptake assay including formazan test R20S C58T/ G59C non-conserved region ACV, FOS plaque reduction assay N23S A68G non-conserved region ACV, FOS R26H G77A non-conserved region ACV, FOS modified dye uptake assay including formazan test
2 A28V C83T non-conserved region ACV plaque reduction assay L29V T85G non-conserved region ACV, FOS plaque reduction assay R30C C88T non-conserved region ACV, FOS plaque reduction assay R32C C94T non-conserved region ACV, FOS dye uptake assay Q35deletion deletion (CAA) nt non-conserved region ACV, FOS modified dye uptake assay including formazan test K36D A106G/ A108T/C non-conserved region ACV, FOS plaque reduction assay K36deletion deletion (AAA) nt non-conserved region ACV, FOS modified dye uptake assay including formazan test K36E A106G non-conserved region ACV, FOS yield reduction assay (ELVIRA) R41H G122A non-conserved region ACV, FOS L42P T125C non-conserved region ACV, FOS cell growth assay Q44H G132C non-conserved region ACV, FOS modified dye uptake assay including formazan test, G61A G182C ATP-binding site Q67R A200G non-conserved region ACV plaque reduction assay L72V C214T non-conserved region ACV, FOS modified dye uptake assay including formazan test R75C C223T non-conserved region ACV, FOS modified dye uptake assay including formazan test D76N G226A non-conserved region ACV, FOS dye uptake assay
3 D77N G229A non-conserved region ACV dye uptake assay I78F A232T non-conserved region R89Q G266A non-conserved region ACV, FOS A98T G292A non-conserved region ACV, FOS modified dye uptake assay including formazan test I100T T299C non-conserved region ACV, FOS dye uptake assay E111K G331A non-conserved region ACV, FOS plaque reduction assay A118V C353T non-conserved region modified dye uptake assay including formazan test A137T G409A non-conserved region ACV, FOS modified dye uptake assay including formazan test V138F G412T non-conserved region ACV, FOS modified dye uptake assay including formazan test V138I G412A non-conserved region ACV A140V C419T non-conserved region ACV, FOS plaque reduction assay H142Y C424T non-conserved region ACV, FOS dye uptake assay E146G A437G non-conserved region ACV, FOS plaque reduction assay H151Y C451T non-conserved region ACV, FOS dye uptake assay A156P G466C non-conserved region ACV, FOS modified dye uptake assay including formazan test A156V C467T non-conserved region ACV, FOS modified dye uptake assay including formazan test
4 F161L C481T non-conserved region ACV, FOS plaque reduction assay V191A T572C non-conserved region ACV, FOS plaque reduction assay A192V C575T non-conserved region ACV, FOS I214T T641C non-conserved region ACV, FOS yield reduction assay (ELVIRA) D215A A644C non-conserved region ACV, FOS modified dye uptake assay including formazan test D215H G643C non-conserved region ACV, FOS dye uptake assay D215N G643A non-conserved region ACV plaque reduction assay K219T A656C conserved region ACV, FOS modified dye uptake assay including formazan test A233S G697T non-conserved region ACV, FOS modified dye uptake assay including formazan test G240E G719A non-conserved region ACV, FOS A243S G727T non-conserved region ACV, FOS dye uptake assay G251C G751T non-conserved region ACV, FOS W259R T775A non-conserved region ACV, FOS modified dye uptake assay including formazan test A265T G793A non-conserved region ACV, FOS V267L G799T non-conserved region ACV, FOS P268L C803T non-conserved region ACV, FOS dye uptake assay
5 P268M C802A/C803T non-conserved region ACV, FOS modified dye uptake assay including formazan test P268T C802A non-conserved region ACV, FOS G271D G812A non-conserved region ACV, FOS modified dye uptake assay including formazan test A272T G814A non-conserved region ACV, FOS dye uptake assay E273K G817A non-conserved region ACV, FOS plaque reduction assay E273Q G817C non-conserved region ACV, FOS plaque reduction assay P274T C820A non-conserved region ACV, FOS dye uptake assay Q275P A824C non-conserved region ACV, FOS modified dye uptake assay including formazan test S276G A826G non-conserved region ACV, FOS plaque reduction assay S276R C828A non-conserved region ACV, FOS modified dye uptake assay including formazan test N277K C831A/G non-conserved region ACV, FOS modified dye uptake assay including formazan test N277S A830G non-conserved region ACV, FOS modified dye uptake assay including formazan test R281Q G842A non-conserved region ACV, FOS DNA reduction assay D286E C858A conserved region ACV, FOS R293W C877T non-conserved region A294V C881T non-conserved region ACV, FOS dye uptake assay
6 P295T C883A non-conserved region ACV, FOS modified dye uptake assay including formazan test P299S G895A/ C896G non-conserved region ACV, FOS dye uptake assay G302S C958A non-conserved region ACV, FOS plaque reduction assay D303N G907A non-conserved region ACV, FOS plaque reduction assay A309V C926T non-conserved region ACV, FOS modified dye uptake assay including formazan test A316V C947T non-conserved region ACV, FOS modified dye uptake assay including formazan test H323Y C967T non-conserved region ACV, FOS plaque reduction assay L327M C980A non-conserved region ACV, FOS dye uptake assay D328E T984G/A non-conserved region ACV, FOS dye uptake assay S332L T994C/ C995T non-conserved region ACV plating efficiency assay S332P T994C non-conserved region ACV, FOS dye uptake assay A334T G1000A non-conserved region ACV, FOS plaque reduction assay G335D G1004A non-conserved region ACV, FOS modified dye uptake assay including formazan test L341R C1021A/ T1022C non-conserved region ACV, FOS dye uptake assay S345P T1033C non-conserved region ACV, FOS modified dye uptake assay including formazan test, G346W G1036T non-conserved region ACV, FOS modified dye uptake assay including formazan test
7 V348I G1042A non-conserved region ACV, FOS T350I C1049T non-conserved region ACV, FOS modified dye uptake assay including formazan test T350S A1048T non-conserved region V352I G1054A non-conserved region ACV, FOS modified dye uptake assay including formazan test S357C C1070G non-conserved region ACV, FOS plaque reduction assay I358V A1072G non-conserved region ACV, FOS plaque reduction assay D363N G1087A non-conserved region ACV, FOS modified dye uptake assay including formazan test L364P T1091C non-conserved region ACV M367T T1100C non-conserved region ACV dye uptake assay G373E G1118A non-conserved region ACV, FOS modified dye uptake assay including formazan test E374A A1124C non-conserved region ACV dye uptake assay A375P A1123C non-conserved region ACV plaque reduction assay N376H A1126C non-conserved region ACV, FOS N376P A1126C/ A1123C non-conserved region ACV, FOS cell growth assay N376T A1127C non-conserved region
Domain Antivirals tested Test methods for phenotype References. Aciclovir (ACV), Foscarnet (FOS) ACV, FOS ACV,
Sauerbrei A, Bohn-Wippert K, Kaspar M, Krumbholz A, Karrasch M, Zell R. 2015. Database on natural polymorphisms and resistance-related non-synonymous mutations in thymidine kinase and DNA polymerase genes
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