Domain Antivirals tested Test methods for phenotype References. Aciclovir (ACV), Foscarnet (FOS) ACV, FOS ACV,

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1 Sauerbrei A, Bohn-Wippert K, Kaspar M, Krumbholz A, Karrasch M, Zell R Database on natural polymorphisms and resistance-related non-synonymous mutations in thymidine kinase and DNA polymerase genes of herpes simplex virus types 1 and 2. J Antimicrob Chemother in press, doi: /jac/dkv285, PMID: Supplementary Table 6: Natural polymorphisms in DNA pol of HSV-2 as aa changes including nt changes, their location, antivirals ed, methods for phenotypic ing and references. Amino acid change Nucleotide change Domain Antivirals ed Test methods for phenotype References A9T G25A (pre NH2-terminal domain) Aciclovir (ACV), Foscarnet (FOS) plaque reduction assay G12R G34C (pre NH2-terminal domain) P15S C43T plaque reduction assay ACV, P15R C43T Brivudin (BVDU), FOS, Penciclovir (PCV), Cidofovir (CDV) R18P G53C plaque reduction assay Q34H G102T/C A36G C107G P37L C110T plaque reduction assay C40W C120G R41H G122A

2 Q43R C127A/ A128G plaque reduction assay H49Y C145T plaque reduction assay T53A A157G plaque reduction assay L60P T179C plaque reduction assay Q64R A191G plaque reduction assay R101W C301T plaque reduction assay L128S C381A/ T382G plaque reduction assay E139K G415A plaque reduction assay T144P A430C plaque reduction assay I153T T458C plaque reduction assay T202M C605T R203Q G608A

3 L229M C685A plaque reduction assay A232T G694A A251V C752T plaque reduction assay N286Y A856T I291V A871G plaque reduction assay A300T G898A G324E G971A plaque reduction assay F336L T1006C plaque reduction assay T347M C1040T plaque reduction assay Q507H G1521C/T (3'-5'-exonuclease domain) plaque reduction assay A547V C1640T conserved region d-region C (3'-5'-exonuclease domain) R628C C1882T plaque reduction assay

4 G646S G1936T plaque reduction assay D671N G2011A plaque reduction assay D676G C2028G plaque reduction assay D676N G2026A plaque reduction assay (DD) aa (GACGAC) nt plaque reduction assay (DED) aa (GACGAG GAC) nt plaque reduction assay E678G A2033G ACV, PCV, FOS plaque reduction assay G680R G2039A plaque reduction assay G680D G2039A plaque reduction assay (GD) aa (GACGAG) nt plaque reduction assay E682G A2045G E682K A2045G plaque reduction assay

5 (ED) aa (GAGGAC) nt (DGDE) aa (GACGGGGAC GAG) nt plaque reduction assay (DGDE) aa (GACGGGGAC GAG) nt plaque reduction assay (GD) aa (GGCGAC) nt D708N G2100A conserved region II D716N G2146A conserved region II E746K G2236A plaque reduction assay Q799R G2396A (fingers domain) plaque reduction assay T801P A2401C (fingers domain) plaque reduction assay, E803K G2407A (fingers domain) plaque reduction assay A869D C2606A A879V C2636T

6 P880H C2639A ACV, BVDU, PCV, FOS, CDV T903M C2708T plaque reduction assay A904G C2711G plaque reduction assay A905E C2714A plaque reduction assay M910I G2730A/C/T K913M A2738T T987S C2960G L995F A2985C/T ACV, BVDU, PCV, FOS, CDV A1000T G2998A plaque reduction assay R1025H G3074A I1026S T3077G ACV, BVDU, PCV, FOS, CDV A1083T G3247A plaque reduction assay

7 (PGDEPA) aa (CCAGGGGAC GAGCCCGCC) nt plaque reduction assay (APGDEPA) aa (GCCCCAGGG GACGAGCCC GCC) nt A1114V C3341T plaque reduction assay E1149G A3446G plaque reduction assay A1156T G3466A plaque reduction assay A1209V C3626T A1223V C3668T plaque reduction assay R1230Q G3689A

plaque reduction assay, modified dye uptake assay including formazan test, dye uptake assay

plaque reduction assay, modified dye uptake assay including formazan test, dye uptake assay Sauerbrei A, Bohn-Wippert K, Kaspar M, Krumbholz A, Karrasch M, Zell R. 2015. Database on natural polymorphisms and resistance-related non-synonymous mutations in thymidine kinase and DNA polymerase genes

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