Warranty...i. Introduction... 1

Transcription

1 USER MANUAL

2 Contents Warranty... Introducton... 1 Gettng Started... 1 Intal Inspecton... 1 Battery Installaton... 1 Setup... 2 Keypad... 9 Man Dsplay Menu Layout Alpha/Numerc Entry System Menu Date/Tme GLP Language Item # Rev D Drawng # A March YSI Incorporated. The YSI logo and Confdence Soluton are regstered trademarks of YSI Incorporated. Teflon s a regstered trademark of E. I. du Pont de Nemours and Company. Mcrosoft and Wndows are regstered trademarks of the Mcrosoft Corporaton n the Unted States and other countres. Pentum s a regstered trademark of Intel Corporaton. Radx Pont Loggng Auto Shutoff Backlght SW Verson (Software Verson) Seral # Unt ID Parameters: Setup, Dsplay, Auto Stable, and Calbraton Temperature... 20

3 Dssolved Oxygen (DO) Barometer Conductvty ph ORP Ammonum, Ntrate, Chlorde Takng Measurements Dssolved Oxygen Conductvty ph/orp Ammonum, Ntrate, and Chlorde Loggng Data Fles and Ste Lsts Fle Memory Vewng Saved Data Ste Lst Folder Delete Data Sensor Mantenance Sensor Storage Troubleshootng Help Error Messages Dssolved Oxygen Restore Default Calbraton Values Accessores / Part Numbers Declaraton of Conformty Recyclng Contact Informaton Orderng and Techncal Support Servce Informaton Data Manager Desktop Software Usng the Communcatons Saddle Manage Logged Data Real Tme Studes Confgure Instruments Care, Mantenance, and Storage Updatng Instrument Frmware General Mantenance... 58

4 Wa r r a n t y Ths page left ntentonally blank The YSI Professonal Plus Instrument (Pro Plus) s warranted for three (3) years from date of purchase by the end user aganst defects n materals and workmanshp, exclusve of batteres and any damage caused by defectve batteres. Pro Plus feld cables are warranted for two (2) years from date of purchase by the end user aganst defects n materal and workmanshp (6 months for non-feld rugged cables*). Pro Plus sensors (ph, ORP, ph/orp combo, Polarographc DO) are warranted for one (1) year from date of purchase by the end user aganst defects n materal and workmanshp (6 months for ammonum**, ntrate**, chlorde**, and Galvanc DO). Pro Plus systems (nstrument, cables & sensors) are warranted for 90 days from date of purchase by the end user aganst defects n materal and workmanshp when purchased by rental agences for rental purposes. Wthn the warranty perod, YSI wll repar or replace, at ts sole dscreton, free of charge, any product that YSI determnes to be covered by ths warranty. To exercse ths warranty, call your local YSI representatve, or contact YSI Customer Servce n Yellow Sprngs, Oho at , or vst www. YSI.com (Support tab) for a Product Return Form. Send the product and proof of purchase, transportaton prepad, to the Authorzed Servce Center selected by YSI. Repar or replacement wll be made and the product returned, transportaton prepad. Repared or replaced products are warranted for the balance of the orgnal warranty perod, or at least 90 days from date of repar or replacement. LIMITATION OF WARRANTY Ths Warranty does not apply to any YSI product damage or falure caused by: 1. falure to nstall, operate or use the product n accordance wth YSI s wrtten nstructons; 2. abuse or msuse of the product; 3. falure to mantan the product n accordance wth YSI s wrtten nstructons or standard ndustry procedure; 4. any mproper repars to the product; 5. use by you of defectve or mproper components or parts n servcng or reparng the product; 6. modfcaton of the product n any way not expressly authorzed by YSI. THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY. IN NO EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY.

5 * The warranty perod for the non-feld rugged cables (605107, , , , , ) s lsted as 6 months. However, the true workng lfe of these sensors may be 3-9 months dependng on storage and usage n solutons other than clean aqueous samples. ** The warranty for the ammonum, ntrate, and chlorde sensors (605104, , ) s lsted as 6 months. However, the true workng lfe of these sensors may be 3-9 months dependng on storage and usage n solutons other than clean aqueous samples. Introducton Thank you for purchasng the YSI Professonal Plus (Pro Plus). The Pro Plus features a waterproof (IP-67) case, backlt dsplay and keypad, user-selectable cable optons, USB connectvty, large memory wth extensve ste lst capabltes, and a rugged, rubber over-molded case. Readng the entre manual before use s recommended for an overall understandng of the nstrument s features. Gettng Started Intal Inspecton Carefully unpack the nstrument and accessores and nspect for damage. Compare receved parts wth tems on the packng lst. If any parts or materals are damaged, contact YSI Customer Servce at ( ) or the authorzed YSI dstrbutor from whom the nstrument was purchased. Battery Installaton The Pro Plus requres (2) alkalne C-cell batteres whch are ncluded wth the purchase of a new nstrument. Battery lfe depends on parameters and usage. Under normal condtons, battery lfe s approxmately 80 hours for contnuous use at room temperature. To nstall or replace the batteres: Turn the nstrument over to vew the battery cover on the back. Unscrew the four captve battery cover screws. Remove the battery cover and nstall the new batteres, ensurng correct polarty algnment on the nstrument or the removed cover. (Fgure 1) Replace the battery cover on the back of the nstrument and tghten the four screws. Do NOT over-tghten. Fgure 1. Pro Plus wth battery cover removed. Notce battery symbols ndcatng polartes. 1

6 Batteres must be nstalled n the nstrument even f powerng the unt va the USB connecton. Ths wll retan the correct date and tme f the PC s turned off. If the USB power s dsconnected and there are no batteres n the nstrument, the date and tme wll need to be reset upon subsequent power on. NOTE - On subsequent battery changes you wll have approxmately 2 mnutes to change the batteres before the clock resets. If the clock resets, the nstrument wll automatcally brng up the Date/Tme menu the next tme t s powered on n order to update ths nformaton. Ths s mportant, especally f you ntend to log data! Setup The Pro Plus nstrument has several compatble feld-rugged cable/sensor optons, each wth temperature: Cable: Cable number x Cable number x Cable number x Cable number x Cable number x Cable number x Cable number x Cable number x Avalable Sensors: DO/temp ( for lab BOD) Conductvty/temp ISE*/temp ISE*/ISE*/temp ISE*/DO/temp ISE*/conductvty/temp DO/conductvty/temp DO/conductvty/ISE*/ISE*/temp (Quatro**) *ISE (Ion Selectve Electrode) notates a port that can accept ph, ORP, Ammonum, Ntrate, Chlorde, and, n some cases, a ph/orp combnaton sensor. **Cable wll be referred to as a Quatro cable throughout ths manual. All cables come n standard lengths of 1, 4, 10, 20, and 30-meters (3.28, 13, 32.8, 65.6, and 98.4-feet) wth optons for specal order lengths up to 100-meters (328-feet) on the x cables. Contact YSI or your local representatve for addtonal nformaton. recommended for lab use or controlled condtons where a more rugged, feld cable s not necessary. These cables nclude: Cable number Cable number Cable number Cable number Cable number Cable number meter cable; sngle-juncton ph sensor 4-meter cable; sngle-juncton ph sensor 1-meter cable; sngle-juncton ORP sensor 4-meter cable; sngle-juncton ORP sensor 1-meter cable; sngle-juncton ph/orp sensors 4-meter cable; sngle-juncton ph/orp sensors Standard Pro Seres SENSOR Installaton Throughout the manual, the term sensor refers to the removable porton or electrode sensng porton of the cable assembly. For example, the DO sensor or ph sensor s the part that can be removed from a feld cable and replaced wth a new sensor. The conductvty sensor s not removable from a non-quatro cable but stll refers to the sensng porton and wll be referred to as a sensor. Ths secton covers most of the sensor nstallatons on a Professonal Seres cable bulkhead ncludng the followng sensors: Polarographc DO (black) Galvanc DO (gray) ph ph/orp Chlorde ORP Ammonum Ntrate See the next secton of ths manual for nstallaton nstructons for the Quatro cable s Conductvty/Temperature sensor. Dual sensor bulkhead ports are numbered 1 and 2, see fgure to the left. Please refer to the followng tables to determne correct sensor nstallaton nto each port of a two port cable. In addton there are several cable optons wth bult n sensors for the measurement of ph and ORP that are not consdered feld-rugged (nonreplaceable sensors, less rugged sngle-juncton sensors). These cables are 2 3

7 1010 dual cable Port 1 Optons ph ORP ph or ph/orp* ammonum chlorde ntrate Port 2 Optons ph ORP ph or ph/orp* ammonum chlorde ntrate none (port plug) * If usng a cable, a sensor must be nstalled n port 1 for correct operaton. If you nstall a ph/orp combo sensor nto a cable, ORP wll not be measured. It s not recommended to use a ph/orp combo sensor on a cable. Port 1 Optons Port 2 Optons ph Polarographc DO ORP Galvanc DO ph or ph/orp none (port plug) 1020 dual cable ammonum chlorde ntrate none (port plug) If usng a 1020 cable, nstall a ph, ORP, ph/orp, Ammonum, Ntrate or Chlorde sensor n port 1 and a DO sensor n port 2. Quatro Cable (pn: ) Port 1 Optons Port 2 Optons DO Port Optons ph ph Polarographc DO ORP ORP Galvanc DO ph or ph/ ORP* ammonum chlorde ntrate ph or ph/ ORP* ammonum chlorde ntrate none (port plug) none (port plug) CT Port Optons 5560 Conductvty/ Temperature sensor only * If usng a Quatro cable, a sensor must be nstalled n port 1 for correct operaton of port 2. If you nstall a ph/orp combo sensor nto a Quatro cable, ORP wll not be measured. It s not recommended to use a ph/orp combo sensor on a Quatro cable. Before nstallng ether dssolved oxygen sensor, the nstrument must be confgured for the sensor beng nstalled. See the Setup - Dssolved Oxygen secton of ths manual for nstrument confguraton nstructons. Falure to do ths may result n damage not covered under warranty. If usng a ph/orp combnaton probe on a or cable you can report both ph and ORP. However, t s recommended to set ISE1 as ph and ISE2 as ORP n the Sensor Setup menu. The Quatro cable bulkheads are labeled 1, 2, DO, and CT, see fgure to the left. All sensors except the Conductvty/Temperature sensor can be nstalled followng the Standard Pro Seres Sensor Installaton nstructons. Conductvty/Temperature sensor nstallaton s descrbed n the next secton. For ease of nstallaton, YSI recommends that you nstall a sensor nto port 1 frst; followed by DO nstallaton, then port 2, and lastly C/T. Frst, ensure both the sensor connector and sensor port on the cable are clean and dry. To connect the sensor, grasp the sensor wth one hand and the sensor connecton end of the cable (bulkhead) n the other. Push the sensor nto the connector on the cable untl t s properly seated and only one o-rng s vsble. Falure to properly seat the probe may result n damage. Twst the sensor clockwse to engage threads and fnger tghten (Fgure 2). Do not use a tool. Ths connecton s waterproof. Please refer to the sensor nstallaton sheet that s ncluded wth each sensor for detaled nstructons. 4 5

8 Sensor Guard Fgure 2. The mage on the left shows a clean, dry sensor beng algned wth the bulkhead. On the rght, the sensor has been pushed nto the bulkhead and s beng screwed nto place. Fgure 3. The sensor(s) wll nstall drectly n the cable bulkhead. Once nstalled, the sensor guard wll protect the sensor durng samplng (DO cap membrane not shown). Conductvty/Temperature sensor Installaton n a Quatro Cable As mentoned, the nstallaton of the Conductvty/Temperature sensor (model 5560) n a Quatro cable s dfferent from all other Pro Seres sensor nstallatons. Follow these nstructons when nstallng a conductvty/temperature sensor n a Quatro cable: Locate the C/T port and, f replacng, remove the old sensor usng the nstallaton tool to loosen the stanless steel retanng nut. Once the stanless steel retanng nut has been completely unscrewed from the bulkhead, remove the old sensor from the bulkhead by pullng the sensor straght out of the bulkhead. Apply a thn coat of o-rng lubrcant (suppled wth the sensor) to the o-rngs on the connector sde of the new sensor. Sensor Cable Bulkhead Vsually nspect the port for mosture. If mosture s found, t must be completely dred pror to sensor nstallaton Algn the connectors of the new sensor and the port. Wth connectors algned, push the sensor n towards the bulkhead untl you feel the sensor seat n ts port. You wll experence some resstance as you push the sensor nward, ths s normal Once you feel the sensor seat nto the port, gently rotate the stanless steel sensor nut clockwse wth your fngers, Do not use the tool. The nut must be screwed n by hand. If the nut s dffcult to turn, STOP, as ths may ndcate cross threadng. If you feel resstance or cross threadng at any pont, unscrew the nut and try agan untl you are able to screw the nut down completely wthout feelng any resstance. Damage to your cable/ sensor may occur f you force the parts together. Once completely nstalled, the nut wll seat flat aganst the bulkhead. At ths pont, use the tool that was ncluded wth the sensor to turn the nut an addtonal ¼ to ½ turn so t cannot come loose (fgure 4). DO NOT over tghten. Do not cross thread the sensor nut. Seat nut on face of bulkhead. Do not over tghten. Please refer to the sensor nstallaton sheet that s ncluded wth the conductvty/ temperature sensor for detaled nstructons. Installng Port Plugs n Unused Ports Fgure 4. Installaton tool used to tghten stanless steel retanng nut of 5560 conductvty/temperature sensor. As necessary, nstall a port plug nto any port that does not have an nstalled sensor. Ths wll protect the bulkhead from water damage. Port plugs and a tube of o-rng lubrcant are ncluded wth all Quatro cables. These tems can be ordered separately f needed. To nstall a port plug, apply a thn coat of o-rng lubrcant to the two o-rngs on the port plug. After applcaton, there should be a thn coat of o-rng lubrcant on the o-rngs. Remove any excess o-rng lubrcant from the o-rng and/or port plug wth a lens cleanng tssue. Next, nsert the plug nto an empty port on the bulkhead and press frmly untl seated. Then, turn the plug clockwse to engage the threads and fnger-tghten untl the plug s nstalled completely. Do not use a tool to tghten the plug. 6 7

9 Connectng the Cable to an Instrument To connect a cable, algn the keys on the cable connector to the slots on the nstrument connector. Push together frmly, then twst the outer rng untl t locks nto place (fgure 5). Ths connecton s water-proof. Keypad Fgure 5. Note the keyed connector. The cable and nstrument connectors can only be mated once the keys are properly algned. Sensor Storage When a sensor s not nstalled, the sensor and cable sensor connectors are NOT water-proof. Do not submerge the cable wthout a sensor or port plug nstalled n all avalable ports. When the cable s dsconnected, the cable s nstrument connector and the connector on the nstrument mantan an IP-67 ratng. The cable assembly s suppled wth a storage contaner, or sleeve, that nstalls on to the cable. The contaner s used for short-term storage (less than 30 days). Be sure to keep a small amount of mosture (tap water) n the contaner durng storage. Ths s done to mantan a 100% saturated ar envronment whch s deal for short-term sensor storage (see Care, Mantenance, and Storage for more detaled nformaton). Do not submerge the sensors n an aqueous soluton. The ntent s to create a humd ar storage envronment. Number Key Descrpton 1 System Opens System Menu from any screen. Use to adjust system settngs. 2 Sensor Opens Sensor Menu from any screen. Use to enable sensors and dsplay unts. 3 Calbrate Opens Calbrate Menu from any screen. Use to calbrate all parameters except temperature. 4 Fle Opens Fle Menu from any screen. Use to vew data and GLP fles, set up ste and folder lsts, and delete data. 5 Backlght Press to turn the nstrument backlght on and off and to adjust the dsplay contrast when pressed wth the left or rght arrow key. 8 9

10 Number Key Descrpton 6 Rght Arrow Use to navgate rght n alpha/numerc entry screens. Can be pressed smultaneously wth Backlght key to ncrease dsplay contrast. 7 Down Arrow Use to navgate through menus and to navgate down n alpha/numerc entry screens. 8 Power Press to turn the nstrument on. Press and hold for 3 seconds to turn off. 9 Help Press to receve hnts & tps durng operaton. 10 Enter Press to confrm selectons, ncludng alpha/numerc key selectons. 11 Left Arrow Use to navgate left n alpha/numerc entry screens. Press to return to prevous menu n all screens except alpha/numerc entry. Can be pressed smultaneously wth Backlght key to decrease dsplay contrast. 12 Ext/Escape Exts back to Run Screen. When n alpha/numerc entry screen, escapes to prevous menu. 13 Up Arrow Use to navgate through menus and to navgate up n alpha/numerc entry screens. Man Dsplay Press the Power key to turn the nstrument on. The nstrument wll brefly dsplay the splash screen wth the YSI logo then go drectly to the man run screen. The frst tme the nstrument s powered up or f the nstrument has had a battery change (wth batteres removed for more than 2 mnutes), you wll need to set the date and tme. Follow the nstructons under System Menu Date/Tme. The dsplay at the left shows the run mode (man dsplay) wth temperature n C, barometer n mmhg, DO n % and mg/l, and ph as the reported parameters. The date, tme and battery level are ndcated at the bottom of the screen. The loggng preference of Log One Sample at a tme s ndcated at the top of the screen. Ths screen also shows the message lne towards the bottom of the dsplay above the date and tme. In ths case, t doesn t show a message but messages wll appear frequently to ndcate calbraton steps, set date and tme, etc. A USB symbol wll show up on the bottom of the dsplay when connected to a PC through USB wth the communcatons saddle. The nstrument wll dsplay full battery power when t s recevng power through the USB connecton. Menu Layout Contrast the contrast adjustment can be accomplshed by pressng the backlght key and the left or rght arrow key at the same tme. Press Esc at anytme n the menus to escape back to the Run screen. The left arrow can be used to go back to the prevous menu n all screens except alpha/numerc entry screens. You must use Esc to get out of the alpha/numerc screens f you want to ext before fnshng or wthout savng changes. Functons that are enabled appear as a crcle wth a dot or a box wth a check mark Q. Dsabled functons appear as a crcle only or an empty P

11 Alpha/Numerc Entry System Menu Press System menu tems. to access any of the followng The System menu wll allow you to access the setup optons of the nstrument ncludng; Date/ Tme, GLP, Language, Radx Pont, Loggng, Auto-Shutoff, Backlght, SW (Software) Verson, Seral #, and Unt ID. Any tem wth [brackets] shows the current settng nsde the brackets. For nstance, n the example at the left, Radx Pont s currently set to [Decmal]. The brackets wll also gve a quck vsual clue as to what tems can be changed. The numerc screens wll dsplay numbers only (shown on the left). Alpha/ numerc screens wll dsplay numbers across the top and letters along the bottom rows (shown on the rght). Letters appear as a common keyboard arrangement. When an alpha or numerc character s requred, the screen wll show the alpha/ numerc entry screen. To select a character, hghlght t by usng the arrow keys to move the hghlght box over the desred selecton. Then, press Enter on the keypad to confrm the selecton. After confrmng the selecton, t wll appear n the lne at the top of the dsplay. For captal letters or lower case entry, hghlght SHIFT and press Enter on the keypad to change the characters from upper to lower case. Date/Tme Hghlght Date/Tme from the System menu. Press enter to select. Date Format Hghlght and press enter to open a sub menu for selectng the preferred date format: YY/MM/DD, MM/DD/YY, DD/MM/YY, or YY/DD/MM. Date Hghlght and press enter to use the numerc entry screen to set the correct date. To delete the entre lne of the current entry, hghlght ) and press Enter on the keypad. The! symbol functons as a backspace key n the alpha/numerc entry screens by deletng one character at a tme. Use the SPACE functon to add a space between characters. When you have fnshed enterng the correct nformaton (16 character max), hghlght <<<ENTER>>> at the bottom of the screen and press Enter on the keypad to confrm. The key cannot be used to escape to the prevous menu from an alpha/numerc entry screen. Instead, use the key to go back to the prevous menu when n alpha/numerc entry screens Tme Format Hghlght and press enter to open a submenu to select the preferred tme format from 12-hour or 24-hour. Tme Hghlght and press enter to use the numerc entry screen to set the correct tme. The date and tme wll need to be reset f a battery change takes longer than 2 mnutes. When ths occurs, the Date/Tme menu wll automatcally appear upon power up and requre you to set the date and tme.

12 GLP The GLP or Good Laboratory Practce fle saves detaled nformaton about calbratons. It also ncludes dagnostc nformaton about the sensors. Calbratons are logged nto a fle, the GLP, for later revew as needed. A sngle GLP fle s utlzed to store all calbraton records and s capable of storng 500 records. Once the GLP fle s full, the nstrument wll begn to overwrte the oldest record wth each new calbraton record. In order to keep all of your GLP records, perodcally download the GLP to Data Manager and export t to another program. Otherwse, the unt wll overwrte the oldest record once the memory s full. Also, snce Data Manager saves GLP fles under the Unt ID, you must perodcally export and rename the GLP fle on your PC or t wll be overwrtten each tme you upload the GLP fle from the nstrument. Several calbraton parameters are saved for each calbraton record ncludng optonal ones that can be enabled by the user. Standard parameters nclude date/tme stamp, calbraton method, and sensor nformaton. Optonal, user selectable parameters nclude User ID, Probe ID, and User Felds 1 and 2. The sensor specfc nformaton that s saved wth each calbraton pont s dfferent for each sensor. The sensor specfc values saved are: Conductvty Method (Spec Cond, Cond, Salnty) Cal Value (value of calbraton soluton) Sensor Value (Cell Constant) Temperature Reference (User selected n Sensor Setup menu) Temperature Compensaton Coeffcent %/ C (User selected n Sensor Setup menu) TDS Constant (User selected n Sensor Setup menu) Temperature Cal Cell Constant Calbrate Status DO Method (%, mg/l) Cal Value Sensor Value (Sensor Current) Sensor Type (Polarographc/Galvanc) Membrane Type (Teflon Black, PE Yellow, PE Blue) Salnty Mode (user entered value f n Manual Salnty Mode) Temperature Barometer Calbrate Status ph (up to 6 calbraton ponts) Buffer Value Sensor Value (mv) Temperature Slope (mv/ph) Slope (% of deal) Calbrate Status ORP Cal Soluton Value Sensor Value Temperature Calbrate Status Ammonum Buffer Value Sensor Value (mv) Temperature Calbrate Status Chlorde Buffer Value Sensor Value (mv) Temperature Calbrate Status Ntrate Buffer Value Sensor Value (mv) Temperature Calbrate Status 14 15

13 An example of a GLP record (Operaton performed s sngle pont % DO Calbraton) *** Calbrate DO% *** Date 02/03/09 MM/DD/YY Tme 12:14:57PM 12-hour User ID: tech 1 Probe ID 08D Method DO Ar Calbrate Cal Value: % Sensor Value: uA Sensor Type Polarographc Membrane Type 1.25 PE Yellow Salnty Mode Auto Temperature 23.9 C Barometer mmhg Calbrate Status Calbrated GLP Settngs In the System menu, hghlght GLP and press enter to vew and modfy the GLP settngs. Hghlght Optons and press enter to access User ID, Probe ID, User Defned Felds, and Re-Cal Prompt. User ID may be used to dentfy the person calbratng the nstrument. Hghlght User ID and press enter to select, edt, or delete a User ID from a lst of prevously entered IDs. Or, hghlght Add New and press enter to create a new User ID usng the alpha/numerc entry screen. The User ID may also be changed n the Calbraton menu durng the calbraton process. The selected User ID wll be stored n the GLP fle wth each calbraton record. A User ID could be a person s ntals or badge number. The character lmt s 16 characters. Probe ID s stored wth the calbraton record and may be used to dstngush one cable/probe assembly from another, typcally by seral number. Hghlght Include Probe ID and press enter to turn ths functon on (Q) and off (P). Hghlght Probe ID and press enter to add, vew, edt, delete, or select a Probe ID. The Probe ID may also be changed n the Calbraton menu durng the calbraton process. The character lmt s 16 characters. User Felds 1 and 2 are stored wth the calbraton record and may be used to enter other parameters pertnent to the user, such as weather condtons, elevaton, etc. Hghlght Include User Feld 1 or Include User Feld 2 and press enter to turn ths functon on and off. Hghlght User Feld 1 or User Feld 2 and press enter to add, delete, vew, edt, or select a User Feld. The character lmt s 16 characters. When enabled, a prompt for selectng a User Defned Feld wll appear durng the calbraton process. Re-Cal Prompt may be used to remnd the user to perform a calbraton. To set a tme nterval, hghlght the parameter you wsh to be remnded about and press enter to access the numerc entry screen. Enter a value n days and press enter to confrm the remnder tme. To turn off the Re-cal prompt, set the remnder to zero (0) days (ths s the default). The Securty secton of the GLP menu s a password protected area. Ths area ncludes optons to set a new password and to lock access to the calbraton menu. When frst vewng the securty menu, you wll be requred to enter a password. Use the shft on the alpha/numerc screen to swtch to lower case f necessary and enter ys123. Ths s the default password. Protect Cal can be enabled (Q) or dsabled (P). When enabled, the user must know and enter the nstrument s password to enter the calbraton menu opton. Hghlght Protect Cal and press enter to enable or dsable ths feature. Set Password allows a user to set the securty password. Hghlght Set Password, press enter, and use the alpha/numerc entry screen to set the new password. The password can have up to 16 characters. Contact YSI Techncal Support at [email protected] or f you forget or msplace your password. Once a password s set, and the GLP securty screen exted, a password must be entered to make changes under GLP securty. Keep passwords n a safe place

14 Language Radx Pont Loggng The Pro Plus can be confgured to dsplay all text n Englsh, Spansh, French, German, Portuguese, Italan, Norwegan, Smplfed Chnese, Tradtonal Chnese, or Japanese. From the factory, the nstrument ncludes Englsh, Spansh, and French language optons. The other language optons can be downloaded from Once the approprate language fle s n the nstrument, press System, hghlght Language, and press enter. Hghlght the desred language and press enter to confrm. Radx Pont allows the user the opton to choose between a comma or a decmal n numerc dsplays. For example, 1.00 becomes 1,00 when Use Comma s selected. Hghlght Use Decmal or Use Comma and press enter to make your selecton. From the System menu, hghlght Loggng and press enter to vew or change the loggng optons. Loggng optons nclude Use Ste Lst, Use Folder Lst, Contnuous Mode, and Interval. Check the box for Contnuous Mode f you want to log samples contnuously at a specfc tme nterval. To set the length of tme between logged samples, hghlght Interval and press enter. Enter the nterval as HH:MM:SS. Ths nterval wll dsplay at the top of the screen when you select the Start Loggng opton n run mode. To log one sample at a tme, uncheck Contnuous Mode. When Contnuous Mode s unchecked, Log One Sample wll appear at the top of the run screen. Auto Shutoff Auto Shutoff powers the nstrument off after a user specfed tme perod. Hghlght Auto Shutoff and press enter. Usng the alpha/numerc entry screen, enter a value between 0 and 360 mnutes. To dsable auto shutoff, set the value to 0 (zero). Backlght SW Verson (Software Verson) Backlght can be set to Automatc or Manual. Automatc turns the backlght on when you turn the nstrument on and when you press any key. Manual allows you to turn the backlght on or off wth the backlght key. When n Automatc mode, the nstrument wll turn the backlght off 60 seconds after the last key press. The nstrument wll reset the 60 second tme perod every tme a key s pressed. The lghted keypad wll turn off after approxmately 20 seconds. SW Verson shows the nstrument s software verson. The nstrument s software can be updated va There you wll fnd the new software fles and nstructons on how to update the nstrument. There s no need to send the nstrument back to the factory for upgrades. Use Ste Lst and Use Folder Lst are optonal ways of flng or taggng your logged data ponts. If these settngs are enabled, you wll be prompted to select a Ste and/or Folder to tag to the logged data pont. See the Fle and Ste Lsts secton of ths manual for nformaton on creatng Ste and Folder Lsts. Seral # Seral # shows the nstrument s seral number and allows you to match t wth the number engraved on the back of the nstrument s case

15 Unt ID Unt ID s used to dentfy nstruments n the Data Manager software program that was ncluded wth your nstrument. It s also used to dentfy GLP fles, Ste Lsts, Confguraton fles, and Data fles transferred from the nstrument to the PC. The default Unt ID s the nstrument s seral number. To modfy the Unt ID, hghlght Unt ID, press enter and then use the alpha/numerc entry screen. The character lmt s 16 characters. Pa r a m e t e r s : S e t u p, D s p l a y, Auto Stable, and Calbraton The followng secton s separated by parameter and wll dscuss sensor setup, dsplay optons, auto stable features, and calbraton procedures for each parameter. The sectons are separated by parameter due to the versatlty of the Pro Plus. You may focus solely on the parameters of your choce. For the hghest accuracy, calbrate or verfy each sensor regularly. For your convenence, YSI offers 5580 Confdence Soluton whch allows you to check the accuracy of ph, conductvty, and ORP readngs to help determne f a sensor calbraton s necessary. If you receve an error message durng a calbraton that ndcates questonable results, you have the opton to ether accept or declne the calbraton. YSI recommends that you declne a questonable calbraton snce acceptng t may result n erroneous data. After declnng a questonable calbraton, ensure the sensor s clean, the calbraton soluton s good, the calbraton vessel s clean, and that you are enterng the correct calbraton value f enterng manually. Then, try to recalbrate the sensor. If you contnue to have problems, see the Troubleshootng secton of ths manual. Temperature All probe/cable assembles, except the Quatro, have a bult-n temperature sensor. The Quatro cable shps wth a Conductvty/Temperature sensor that must be nstalled on the cable. Temperature calbraton s not requred nor s t avalable. temperature unts of F, C, or K and press enter to confrm the selecton. Only one temperature unt may be dsplayed at a tme. You may also choose not to dsplay temperature. If you choose not to dsplay temperature, other parameters that requre a temperature readng wll stll be temperature compensated. Dssolved Oxygen (DO) DO sensors can be used on X, X, X, and Quatro cables. Preparng the DO Sensor for the Frst Tme The dssolved oxygen sensor s shpped wth a dry, protectve red cap that wll need to be removed before usng. It s very mportant to put a new membrane wth electrolyte soluton on the sensor after removng the red cap. Prepare the membrane soluton accordng to the nstructons on the bottle. After mxng, allow the soluton to st for 1 hour. Ths wll help prevent ar bubbles from later developng under the membrane. Ensure you are usng the correct electrolyte soluton for the correct sensor. Galvanc sensors utlze electrolyte wth a lght blue label and Polarographc sensors utlze electrolyte wth a whte label. The dssolved oxygen sensor s suppled wth cap membranes specfc to the sensor type ordered (Polarographc or Galvanc). 5912, 5913, and 5914 membrane kts are for Galvanc sensors and the 5906, 5908, and 5909 membrane kts are for Polarographc sensors. See the Setup - Dssolved Oxygen secton of ths manual for more nformaton on the dfferent types of membranes avalable from YSI. Remove the red cap by pullng t straght off the sensor tp. Dscard or save for later use durng long term storage. Thoroughly rnse the sensor tp wth dstlled or deonzed water. Fll the cap membrane 3 4 full of electrolyte soluton, then tap the cap wth a fnger to release any trapped ar. Be careful not to touch the membrane porton of the cap. Thread the membrane cap onto the sensor, moderately tght. Do not use a tool. It s typcal for some of the electrolyte soluton to spll over. For detaled nstructons on changng a membrane cap, see the Care, Mantenance, and Storage secton of ths manual. To set the unts, press Sensor, hghlght Dsplay and press enter. Hghlght Temperature and press enter. Hghlght the desred 20 21

16 Setup - Dssolved Oxygen Press Sensor enter., hghlght Setup and press enter. Next, hghlght DO and press Enabled allows you to enable or dsable the Dssolved Oxygen functon. Hghlght Enabled and press enter to actvate(q) or deactvate(p) dssolved oxygen. Dsable dssolved oxygen f you do not have a dssolved oxygen sensor connected to the nstrument. If a sensor s Enabled that sn t connected to the nstrument, the dsplay wll show an unstable, false readng,?????, or next to the unts. Sensor Type sets the type of oxygen sensor beng used: ether Polarographc (black) or Galvanc (grey). Hghlght Sensor Type and press enter. Hghlght the correct sensor type nstalled on the cable and press enter to confrm. If usng a ProBOD sensor/cable assembly, the sensor type should be set to polarographc. The Pro Plus has two compatble sensors for use wth a feld cable: Polarographc Ths sensor has a black sensor body and s engraved wth the model number Galvanc Ths sensor has a grey sensor body and s engraved wth the model number In terms of physcal confguraton, membrane materal, and general performance, YSI Professonal Seres Galvanc dssolved oxygen sensors are exactly lke the Professonal Seres Polarographc sensors. The advantage of usng Galvanc sensors s convenence. Galvanc sensors provde for an nstant-on sensor wthout the need for warm-up tme but ths affects the lfe of the sensor. Polarographc sensors last longer and have a longer warranty but requre a 5-15 mnute warmup tme before use or calbraton. u IMPORTANT The nstrument default settng s Galvanc. Please change the Sensor Type to match the correct sensor. If you observe readngs very close to 0 or extremely hgh readngs (.e. 600%), your Sensor Type settng (Polarographc or Galvanc) may be set ncorrectly and you should mmedately ensure t matches the sensor nstalled on your cable. Membrane sets the type of membrane used on the dssolved oxygen sensor. Hghlght Membrane and press enter. Hghlght the correct membrane type nstalled on the sensor and press enter to confrm. The DO sensor s suppled wth membranes specfc to the sensor type ordered and are color coded as descrbed n the followng tables. Galvanc membrane kts: Item Color Materal Descrpton 5912 Black 1 ml Teflon Tradtonal membrane materal 5913 Yellow 1.25 ml polyethylene Improved response tme and less flow dependence than Teflon Shps standard wth the sensor Blue 2 ml polyethylene Less flow dependence than 1.25 ml but somewhat slower response Polarographc membrane kts: Item Color Materal Descrpton 5906 Black 1 ml Teflon Tradtonal membrane materal 5908 Yellow 1.25 ml polyethylene Improved response tme and less flow dependence than Teflon Shps standard wth the sensor Blue 2 ml polyethylene Less flow dependence than 1.25 ml but somewhat slower response Selectng a Dssolved Oxygen Membrane: Membrane Type Flow Dependence After 4 Mnutes Typcal Response Tme - 95% 5912, Black 60% 18 seconds 5913, Yellow 25% 8 seconds 5914, Blue 18% 17 seconds 22 23

17 Local DO allows for localzed DO% measurements. Ths sets the calbraton value to 100% regardless of the alttude or barometrc pressure. Hghlght Local DO and press enter to enable (Q) or dsable (P) ths functon. Local DO s a method for the Pro Plus to factor n the barometrc pressure on each DO measurement. In essence, f the barometrc pressure changes you wouldn t notce the dfference n the DO% readngs n ar-saturated water or water-saturated ar. Local DO s deal for EU complance. When Local DO s enabled, an L wll appear next to DO% on the run screen. DO mg/l readngs are unaffected by the selecton of DO Local. LDS (Last Dgt Suppresson) rounds the DO value to the nearest tenth;.e mg/l becomes 8.3 mg/l. Hghlght LDS and press enter to enable (Q) or dsable (P) ths functon. Dsplay - Dssolved Oxygen Press Sensor, hghlght Dsplay and press enter. Hghlght DO and press enter. All DO unts can be dsplayed smultaneously. Hghlght the unt(s) and press enter to actvate (Q) or deactvate (P) unts from the run screen. Note - You wll not be able to dsplay dssolved oxygen unless t s Enabled n the Sensor Setup menu frst, see prevous secton. s acheved) and press enter to confrm. The Auto Stable Senstvty can be decreased or ncreased. Hghlght Senstvty and use the left and rght arrow keys to slde the bar. The more senstve you make t (larger black bar) the harder t s to acheve stablty n a changng envronment. The Auto Stable system works by examnng the prevous 5 readngs, computng the percent change n the data and comparng that change aganst a % threshold value. The % threshold value s determned by the Senstvty bar settng. The followng chart can be used as a gude when settng the Senstvty bar. Senstvty selected by User Most Senstve, Senstvty bar s set to the far rght Example: % Data Varance Threshold 0.05% % % % 0 - Least Senstve, Senstvty bar s set to the far left 2.5% DO % wll show DO readngs n a percent scale from 0 to 500%. DO mg/l wll show DO readngs n mllgrams per lter (equvalent to ppm) on a scale from 0 to 50 mg/l. DO ppm wll show DO readngs n parts per mllon (equvalent to mg/l) on a scale from 0 to 50 ppm. Auto Stable - Dssolved Oxygen Auto Stable ndcates when a readng s stable.when Auto Stable s enabled, AS wll blnk next to the parameter untl t s stable. Once the parameter s stable, AS wll stop blnkng. To enable Auto Stable, press Sensor, hghlght Auto Stable and press enter. Hghlght DO and press enter. Hghlght Enabled and/or Audo Enabled (nstrument wll beep when the stablty The nstrument obtaned the followng data: Readng # DO% Readng # DO% Readng # DO% Readng # DO% Readng # DO% The nstrument s programmed to determne the mnmum and maxmum data value over the prevous 5 samples, and to compute the percent dfference between those values. In ths example, that gves a percent change of: % Change = 100 * (( ) / 95.3) % Change = 0.83% In ths example, f the Senstvty bar s set to the far rght, the Auto Stable requrement would not be met and AS would contnue to blnk. However, f the senstvty bar s set to the medan threshold (1.275%), the Auto Stable requrement would be met and AS would dsplay steadly on the dsplay. Wthn the Auto Stable menu, you can also choose to Hold All Readngs for as many parameters as you set for Auto Stable. For nstance, f DO and ph have 24 25

18 Auto Stable and Hold All Readngs enabled, then the dsplay wll hold the readngs once DO and ph have both reached ther Auto Stable settngs. You must press the Esc key to release the held dsplay n order to take subsequent readngs Hold All Readngs must be reactvated after each use! Calbraton - Dssolved Oxygen The Pro Plus offers several optons for calbratng dssolved oxygen: DO% n water saturated ar, DO mg/l and DO ppm n a soluton of known dssolved oxygen determned by a Wnkler Ttraton, and a Zero pont. If performng a zero pont calbraton, you must also perform a %, mg/l, or ppm calbraton followng the zero calbraton. For both ease of use and accuracy, YSI recommends performng the followng 1-pont DO % water saturated ar calbraton: Salnty Correcton The last feature n the Sensor menu s the Salnty correcton value whch s used to calculate the dssolved oxygen mg/l and ammona readngs when a conductvty sensor s not n use. Press Sensor, hghlght Salnty, and press enter. Then, use the numerc entry screen to enter the Salnty value of the water you wll be testng from 0 to 70 ppt. If usng a cable wth a conductvty sensor, the salnty measured by the conductvty sensor wll be used n the DO and ammona mg/l calculatons and As Measured wll be dsplayed next to Salnty n the Sensor menu. As the salnty of water ncreases, ts ablty to dssolve oxygen decreases. For example, fully oxygenated 20 C water at sea level wth zero salnty wll hold mg/l of dssolved oxygen. If that same sample had a salnty value of 9 ppt, then t would hold mg/l of dssolved oxygen. Therefore, to obtan accurate mg/l readngs, t s mportant to know the salnty of the water you wll be testng and to nput that value nto the nstrument. The salnty of fresh water s typcally ppt and seawater s typcally 35 ppt. You wll also have the opportunty to enter or modfy the Salnty correcton value durng DO calbraton. It s not necessary to calbrate n both % and mg/l or ppm. Calbratng n % wll smultaneously calbrate mg/l and ppm and vce versa. Calbratng DO % n Water Saturated Ar: 1-Pont Calbraton The suppled sensor storage contaner (a grey sleeve for a sngle port cable or a screw on plastc cup for the dual-port and Quatro cables) can be used for DO calbraton purposes. Mosten the sponge n the storage sleeve or plastc cup wth a small amount of clean water. The sponge should be clean snce bacteral growth may consume oxygen and nterfere wth the calbraton. If usng the cup and you no longer have the sponge, place a small amount of clean water (1/8 nch) n the plastc storage cup nstead. Make sure there are no water droplets on the DO membrane or temperature sensor. Then nstall the storage sleeve or cup over the sensors. The storage sleeve ensures ventng to the atmosphere. If usng the cup, screw t on the cable and then dsengage one or two threads to ensure atmospherc ventng. Make sure the DO and temperature sensors are not mmersed n water. Turn the nstrument on and wat approxmately 5 to 15 mnutes for the storage contaner to become completely saturated and to allow the sensors to stablze. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. User ID wll appear automatcally. Select None f you do not want a User ID stored wth the calbraton. When enabled, these IDs are stored wth each calbraton record n the GLP fle

19 After selectng your User ID and/or Probe ID f approprate, hghlght DO and press enter. Hghlght DO % and press enter to confrm. The nstrument wll use the nternal barometer durng calbraton and wll dsplay ths value n brackets at the top of the dsplay. Hghlght Barometer and press enter to adjust t f needed. If the barometer readng s ncorrect, t s recommended that you calbrate the barometer. Note - the barometer should be readng true barometrc pressure (see Barometer secton for more nformaton on true barometrc pressure). If the value s acceptable, there s no need to change t or perform a barometer calbraton. The Salnty value dsplayed near the top of the screen s ether the salnty correcton value entered n the Sensor menu or the Salnty value as measured by the conductvty sensor n use and enabled. If you are not usng a conductvty sensor, the Salnty correcton value should be the salnty of the water you wll be testng. Hghlght Salnty and press enter to modfy ths settng f necessary. See the Salnty Correcton secton of ths manual for more nformaton. Wat for the temperature and DO% values under Actual Readngs to stablze, then hghlght Accept Calbraton and press enter to calbrate. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled n the GLP menu, you wll be prompted to select these nputs and then press Cal to complete the calbraton. The message lne at the bottom of the screen wll dsplay Calbratng Channel... and then Savng Confguraton.... Calbratng DO% n Water Saturated Ar: 2-Pont Calbraton wth Zero Soluton Place the sensor n a soluton of zero DO. A zero DO soluton can be made by dssolvng approxmately 8-10 grams of sodum sulfte (Na 2 SO 3 ) nto 500 ml tap water or DI water. Mx the soluton thoroughly. It may take the soluton 60 mnutes to be oxygen-free. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght DO and press enter. Hghlght Zero and press enter. Wat for the temperature and DO% values under Actual Readngs to stablze, then press enter to Accept Calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select the felds and then press Cal to complete the calbraton. The screen wll then prompt for a follow-up second pont calbraton. Hghlght DO% and press enter to contnue wth the next calbraton pont. Rnse the sensor of any zero oxygen soluton usng clean water. Then follow the steps under Calbratng DO % n Water Saturated Ar to complete the second pont. Calbratng n mg/l or ppm as a Ttraton: 1-Pont Calbraton Place the sensor nto an adequately strred sample that has been ttrated to determne the dssolved oxygen concentraton. Allow the sensor to stablze. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght DO and press enter. Hghlght DO mg/l or ppm and press enter

20 Calbratng n mg/l or ppm as a Ttraton: 2-Pont Calbraton wth Zero Soluton Place the sensor n a soluton of zero DO. Hghlght Calbraton value and press enter to manually nput the sample s dssolved oxygen value. Hghlght Accept Calbraton and press enter once the temperature and Dssolved Oxygen readngs stablze. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled n the GLP menu, you wll be prompted to select the felds after selectng Accept Calbraton. After makng your selecton, press Cal to complete the calbraton. After completng the calbraton, the message lne wll dsplay Calbratng Channel... and then Savng Confguraton.... A zero DO soluton can be made by dssolvng approxmately 8-10 grams of sodum sulfte (Na 2 SO 3 ) nto 500 ml tap water. Mx the soluton thoroughly. It may take the soluton 60 mnutes to be oxygen-free. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght DO and press enter. Hghlght Zero and press enter. Wat for the temperature and DO% values under Actual Readngs to stablze, then press enter to Accept Calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select the felds and then Press Cal to complete the calbraton. The screen wll then prompt for a follow-up second pont calbraton. Barometer All Professonal Plus nstruments contan an nternal barometer. Dsplay - Barometer Press Sensor, hghlght Dsplay and press enter. Hghlght Barometer and press enter. The measurement unt optons are: mmhg, nhg, mbar, PSI, kpa, or Atm. Only one unt can be dsplayed at a tme. Select None f you do not want to dsplay a barometrc pressure readng. Whether or not you choose to dsplay the barometer readng, the barometrc pressure wll stll be used for calbratng DO% and for compensatng for pressure changes f Local DO s enabled. Calbraton - Barometer The barometer n the nstrument s calbrated at the factory. If the barometer requres calbraton, press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght Barometer and press enter. Hghlght the desred unt and press enter. Hghlght the desred calbraton unts (mg/l or ppm) and press enter to contnue wth the next pont. Rnse the sensor of any zero oxygen soluton usng clean water. To complete the second calbraton pont, follow the steps under Calbratng n mg/l or ppm as a Ttraton: 1-Pont Calbraton

21 Hghlght Calbraton Value and press enter to manually enter the correct true barometrc pressure. Next, hghlght Accept Calbraton, and press enter. If User Feld 1 or 2 are enabled, you wll be prompted to select the felds and then press Cal to complete the calbraton or press Esc to cancel the calbraton. Laboratory barometer readngs are usually true (uncorrected) values of ar pressure and can be used as s for barometer calbraton. Weather servce readngs are usually not true,.e., they are corrected to sea level, and therefore cannot be used untl they are uncorrected. An approxmate formula for ths uncorrecton s below: True BP = [Corrected BP] [2.5 * (Local Alttude n ft. above sea level/100)] Conductvty Conductvty sensors are suppled wth X, X, X, and Quatro cables. Conductvty sensors are bult nto the X, X, and X cables and are not replaceable. Conductvty/Temperature sensors are shpped wth the Quatro cable, must be nstalled, and are replaceable. Setup - Conductvty Press Sensor, hghlght Setup, and press enter. Hghlght Conductvty, press enter. temperature all Specfc Conductance values are compensated to. The default s 25 C. To change the Reference Temperature, hghlght Temp Ref and press enter. Use the numerc entry screen to enter a new value between and C. Next, hghlght <<<ENTER>>> at the bottom of the screen and press enter on the keypad to confrm. %/ C (Percent per Degree Celsus) s the temperature coeffcent used to calculate temperature compensated Specfc Conductance. The default s 1.91% whch s based on KCl standards. To change the temperature coeffcent, hghlght %/ C and press enter. Use the numerc entry screen to enter a new value between 0 and 4%. Next, hghlght <<<ENTER>>> at the bottom of the screen and press Enter on the keypad to confrm. TDS Constant s a multpler used to calculate an estmated TDS (Total Dssolved Solds) value from conductvty. The multpler s used to convert Specfc Conductance n ms/cm to TDS n g/l. The default value s Ths multpler s hghly dependent on the nature of the onc speces present n the water sample. To be assured of moderate accuracy for the converson, you must determne a multpler for the water at your samplng ste. Use the followng procedure to determne the multpler for a specfc sample: Determne the specfc conductance of a water sample from the ste; Flter a porton of water from the ste; Completely evaporate the water from a carefully measured volume of the fltered sample to yeld a dry sold; Accurately wegh the remanng sold; Dvde the weght of the sold (n grams) by the volume of water used (n lters) to yeld the TDS value n g/l for ths ste; Dvde the TDS value n g/l by the specfc conductance of the water n ms/cm to yeld the converson multpler. Be certan to use the correct unts. Enabled allows you to enable or dsable the conductvty measurement. Hghlght Enabled and press enter to actvate (Q) or deactvate (P) conductvty. Dsable conductvty f you do not have a conductvty sensor connected to the nstrument. If a sensor s Enabled that sn t connected to the nstrument, the dsplay wll show an unstable, false readng next to the unts. If the nature of the onc speces at the ste changes between samplng studes, the TDS values wll be n error. TDS cannot be calculated accurately from specfc conductance unless the make-up of the chemcal speces n the water remans constant. To change the multpler, hghlght TDS Constant and press enter. Use the numerc entry screen to enter a new value between 0 and Hghlght <<<ENTER>>> at the bottom of the screen and press Enter on the keypad to confrm. Temp Ref (Temperature Reference) s the reference temperature used for calculatng temperature compensated Specfc Conductance. Ths wll be the 32 33

22 Dsplay - Conductvty Press Sensor, hghlght Dsplay and press enter. Hghlght Conductvty and press enter. Hghlght Sp. Conductance (Specfc Conductance), Conductvty, Salnty, TDS, or Resstvty, and press enter to select the reportng unts for each parameter. One reportng unt per parameter may be enabled. To dsable a parameter, select None. You wll not be able to dsplay any of these parameters unless the Conductvty sensor s Enabled n the Sensor Setup menu frst. Sp. Conductance can be dsplayed n us/cm or ms/cm. Specfc conductance s temperature compensated conductvty. Conductvty can be dsplayed n us/cm or ms/ cm. Conductvty s the measure of a soluton s ablty to conduct an electrcal current. Unlke specfc conductance, conductvty s a drect readng wthout any temperature compensaton. Salnty can be dsplayed n ppt (parts per thousand) or PSU (practcal salnty unts). The unts are equvalent as both use the Practcal Salnty Scale for calculaton. TDS can be dsplayed n mg/l (mllgrams per lter), g/l (grams per lter), or kg/l (klograms per lter). Resstvty can be dsplayed n ohm-cm (ohms per centmeter), kohm-cm (klo ohms per centmeter), or Mohm-cm (mega ohms per centmeter). Auto Stable - Conductvty Press Sensor, hghlght Auto Stable and press enter. Hghlght Conductvty and press enter. The Auto Stable Senstvty can be decreased or ncreased. Hghlght Senstvty and use the left and rght arrow keys to slde the bar. The more senstve you make t (larger black bar) the harder t s to acheve stablty n a changng envronment. The Auto Stable system works by examnng the prevous 5 readngs, computng the percent change n the data and comparng that change aganst a % threshold value. The % threshold value s determned by the Senstvty bar settng. The followng chart can be used as a gude when settng the Senstvty bar. Senstvty selected by User Most Senstve, Senstvty bar s set to the far rght % Data Varance Threshold 0.025% % % % 0 - Least Senstve, Senstvty bar s set to the far left 1.5% Wthn the Auto Stable menu, you can also choose to Hold All Readngs for as many parameters as you set for Auto Stable. For nstance, f conductvty and DO have Auto Stable and Hold All Readngs enabled, then the dsplay wll hold the readngs once conductvty and DO have both reached ther Auto Stable settngs. You must press the Esc key to release the held dsplay n order to take subsequent readngs. Hold All Readngs must be reactvated after each use! Auto Stable ndcates when a readng s stable. Hghlght Enabled and/or Audo Enabled (nstrument wll beep when the stablty s acheved) and press enter enable (Q) or dsable (P). When Auto Stable s enabled, AS wll blnk next to the parameter untl t s stable. Once the parameter s stable, AS wll stop blnkng

23 Calbraton - Conductvty The ISE/conductvty cable has a specalzed calbraton contaner that resembles a large test tube. Ths calbraton chamber can be used to calbrate the conductvty sensor wth an ISE sensor nstalled. A rng-stand should be used to support ths chamber. Choose the unts n ether SPC-us/cm, C-us/cm or SPC-ms/cm, C-ms/cm and press enter. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. User ID wll appear automatcally. Select None f you do not want a User ID stored wth the calbraton. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the User ID and/or Probe ID f approprate, hghlght Conductvty and press enter. Hghlght Calbraton value and press enter to nput the value of the calbraton standard. Then, once the temperature and conductvty readngs stablze, hghlght Accept Calbraton and press enter. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled n the GLP menu, you wll be prompted to select the felds and then press Cal to complete the calbraton. After completng the calbraton, the message lne at the bottom of the screen wll dsplay Calbratng Channel... and then Savng Confguraton.... Calbratng n Salnty Hghlght the desred calbraton method; Sp. Conductance, Conductvty, or Salnty and press enter. YSI recommends calbratng n specfc conductance for greatest ease. Place the sensor nto a salnty calbraton soluton. The soluton must cover the holes of the conductvty sensor that are closest to the cable. Ensure the entre conductvty sensor s submerged n the soluton or the nstrument wll read approxmately of half the expected value! Select SAL ppt or SAL PSU and press enter. Calbratng n Specfc (Sp.) Conductance or Conductvty Place the sensor nto a fresh, traceable conductvty calbraton soluton. The soluton must cover the holes of the conductvty sensor that are closest to the cable. Ensure the entre conductvty sensor s submerged n the soluton or the nstrument wll read approxmately of half the expected value! Hghlght Calbraton value and press enter to nput the value of the calbraton standard. Then, once the temperature and conductvty readngs stablze, hghlght Accept Calbraton and press enter. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select the felds and then press Cal to complete the calbraton

24 ph Dsplay - ph ph sensors can be used on X, X, X, X, and Quatro cables. Press Sensor enter., hghlght Dsplay and press If usng a ph/orp combnaton sensor on a or cable you can report both ph and ORP by confgurng ISE1 as ph and ISE2 as ORP n the Sensor Setup menu. The ph/orp combnaton sensor s not recommended for use on a or Quatro cable. If used on one of these cable, only ph wll be reported and ORP wll not be measured. Hghlght ISE (ph) and press enter. You wll not be able to Dsplay the sensor unless t s Enabled n the Sensor Setup menu. Hghlght ph and/or ph mv, press enter to enable (Q) or dsable (P). Both can be reported at the same tme. Setup - ph Press Sensor, hghlght Setup, press enter. Hghlght ISE1 f usng a 60510, , or cable. If usng a or Quatro cable, hghlght ISE1 f the ph sensor s nstalled n port 1 or hghlght ISE2 f the ph sensor s nstalled n port 2(a sensor must be nstalled n port 1 for port 2 to operate). Press enter. Enabled allows you to enable or dsable the ISE functon and select whch ISE sensor s nstalled on the cable. Hghlght Enabled and press enter to enable (Q) or dsable (P) the ISE you selected prevously (ether ISE1 or ISE2). Dsable the ISE functon(s) f you do not have a ISE sensor connected to the nstrument. After enablng the ISE functon, ensure that t s set to ph as shown n the left screen shot. If necessary, hghlght ph and press enter to set the ISE to ph. Hghlghtng ph[usa] and pressng enter wll also allow you to select the values for auto buffer recognton whch are used durng calbraton. The buffer optons are USA (4, 7, 10), NIST (4.01, 6.86, 9.18), and User-Defned. The selected opton wll be dsplayed n [brackets]. Auto Stable - ph Press Sensor and press enter., hghlght Auto Stable and press enter. Hghlght ISE (ph) Auto Stable ndcates when a readng s stable. Hghlght Enabled and/or Audo Enabled (nstrument wll beep when the stablty s acheved) and press enter enable (Q) or dsable (P). When Auto Stable s enabled, AS wll blnk next to the parameter untl t s stable. Once the parameter s stable, AS wll stop blnkng. The Auto Stable Senstvty can be decreased or ncreased. Hghlght Senstvty and use the left and rght arrow keys to slde the bar. The more senstve you make t (larger black bar) the harder t s to acheve stablty n a changng envronment. The Auto Stable system works by examnng the prevous 5 readngs, computng the percent change n the data and comparng that change aganst a % threshold value. The % threshold value s determned by the Senstvty bar settng. The followng chart can be used as a gude when settng the Senstvty bar. If a sensor s Enabled that sn t connected to the nstrument, the dsplay wll show an unstable false readng,?????, or next to the unts

25 Senstvty selected by User Most Senstve, Senstvty bar s set to the far rght % Data Varance Threshold 0.025% % % % 0 - Least Senstve, Senstvty bar s set to the far left 0.15% Wthn the Auto Stable menu, you can also choose to Hold All Readngs for as many parameters as you set for Auto Stable. For nstance, f ORP and ph have Auto Stable enabled and Hold All Readngs s enabled, then the dsplay wll hold the readngs once ORP and ph have both reached ther Auto Stable settngs. You must press the Esc key to release the held dsplay n order to take subsequent readngs. Hold All Readngs must be reactvated after each use! Calbraton - ph Calbraton can be accomplshed n any buffer order. ph 7 buffer should be used regardless of how many calbraton ponts you use but t does not have to be used frst. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. User ID wll appear automatcally. Select None f you do not want a User ID stored wth the calbraton. When enabled, these IDs are stored wth each calbraton record n the GLP fle. n the Sensor Setup menu may be ncorrect. If necessary, hghlght the Calbraton Value and press enter to nput the correct buffer value. Once the ph and temperature readngs stablze, hghlght Accept Calbraton and press enter to accept the frst calbraton pont. The message lne wll then dsplay Ready for pont 2. If you do not wsh to perform a second pont, press Cal to fnalze the calbraton. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select these felds and then press Cal to fnalze the calbraton. To contnue wth the 2nd pont, place the sensor n the second buffer soluton. The nstrument should automatcally recognze the second buffer value and dsplay t at the top of the screen. If necessary, hghlght the Calbraton Value and press enter to nput the correct buffer value. Once the ph and temperature readngs stablze, hghlght Accept Calbraton and press enter to confrm the second calbraton pont. The message lne wll then dsplay Ready for pont 3 and you can contnue wth the 3rd calbraton pont f desred. If you do not wsh to perform a 3rd calbraton pont, press Cal to complete the calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select these felds and then press Cal to fnalze the calbraton. Contnue n ths fashon untl the desred number of calbraton ponts s acheved (up to sx). After selectng your User ID and/or Probe ID f approprate, hghlght ISE (ph) and press enter. The message lne wll show the nstrument s Ready for pont 1. The ph calbraton allows up to sx calbraton ponts. Place the sensor n a traceable ph buffer soluton. The nstrument should automatcally recognze the buffer value and dsplay t at the top of the calbraton screen. If the calbraton value s ncorrect, the auto buffer recognton settng u Once you ve acheved the desred number of cal ponts you must press Cal to fnalze the calbraton and to allow the nstrument to update the ph offset and slope. The nstrument wll not take these cal values nto account untl Cal has been pressed.

26 ORP The actual readngs dsplayed durng the calbraton wll NOT reflect the updated calbraton nformaton. These values wll not change untl Cal s pressed to fnalze the calbraton and to update the nstrument. ORP sensors can be used on X, X, X, X, and Quatro cables. If usng a ph/orp combnaton sensor on a or cable you can report both ph and ORP by confgurng ISE1 as ph and ISE2 as ORP n the Sensor Setup menu. The ph/orp combnaton sensor s not recommended for use on a or Quatro cable. If used on one of these cable, only ph wll be reported and ORP wll not be measured. Setup - ORP Press Sensor, hghlght Setup, press enter. Hghlght ISE1 f usng a (ORP sensor) on a 60510, , or cable. Hghlght ISE2 s usng a (ph/orp sensor) on a 60510, , or cable. If usng a or Quatro cable, hghlght ISE1 f the ORP sensor s nstalled n port 1 or hghlght ISE2 f the ORP sensor s nstalled n port 2 (a sensor must be nstalled n port 1 for port 2 to operate). Press enter. Enabled allows you to enable or dsable the ISE functon and select whch ISE sensor s nstalled on the cable. Hghlght Enabled and press enter to enable (Q) or dsable (P) the ISE you selected prevously (ether ISE1 or ISE2). After enablng the ISE functon, ensure ORP s selected as the ISE sensor as shown n screen shot to the left. If necessary, hghlght ORP and press enter to set the selected ISE to ORP. Dsplay - ORP Auto Stable - ORP If a sensor s Enabled that sn t connected to the nstrument, the dsplay wll show an unstable false readng,?????, or next to the unts. Press Sensor enter., hghlght Dsplay and press Hghlght ISE (ORP) and press enter. You wll not be able to Dsplay the sensor unless t s Enabled n the Sensor Setup menu. Press enter to enable (Q) or dsable (P) ORP mv. Press Sensor press enter. enter., hghlght Auto Stable and Hghlght ISE (ORP) and press Auto Stable ndcates when a readng s stable. Hghlght Enabled and/or Audo Enabled (nstrument wll beep when the stablty s acheved) and press enter enable (Q) or dsable (P). When Auto Stable s enabled, AS wll blnk next to the parameter untl t s stable. Once the parameter s stable, AS wll stop blnkng. The Auto Stable Senstvty can be decreased or ncreased. Hghlght Senstvty and use the left and rght arrow keys to slde the bar. The more senstve you make t (larger black bar) the harder t s to acheve stablty n a changng envronment. The Auto Stable system works by examnng the prevous 5 readngs, computng the percent change n the data and comparng that change aganst a % threshold value. The % threshold value s determned by the Senstvty bar settng. The followng chart can be used as a gude when settng the Senstvty bar

27 Senstvty selected by User Most Senstve, Senstvty bar s set to the far rght % Data Varance Threshold 0.05% % % % 0 - Least Senstve, Senstvty bar s set to the far left Calbraton - ORP 2.5% Wthn the Auto Stable menu, you can also choose to Hold All Readngs for as many parameters as you set for Auto Stable. For nstance, f ORP and ph have Auto Stable enabled and Hold All Readngs s enabled, then the dsplay wll hold the readngs once ORP and ph have both reached ther Auto Stable settngs. You must press the Esc key to release the held dsplay n order to take subsequent readngs. Hold All Readngs must be reactvated after each use! Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. User ID wll appear automatcally. Select None f you do not want a User ID stored wth the calbraton. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng your User ID and/or Probe ID f approprate, hghlght ISE (ORP) and press enter. The message lne wll show the nstrument s Ready for pont. Place the sensor n a soluton of known ORP and wat for the readngs to stablze. Ammonum, Ntrate, Chlorde Hghlght Calbraton value and press enter to nput the value of the ORP calbraton standard. If usng the YSI Zobell calbraton soluton, the Pro Plus wll automatcally determne the calbraton value. However, the calbraton value should be verfed aganst the chart on the sde of the Zobell bottle. Next, once the temperature and ORP readngs stablze, hghlght Accept Calbraton and press enter to calbrate. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select the felds and then press Cal to complete the calbraton. Ammonum, Ntrate, and Chlorde sensors can be used on X, X, X, X, and Quatro cables. These cables also accommodate ph and ORP sensors so nstrument setup s mportant. WARNING: Ammonum, Ntrate, and Chlorde sensors should only be used at DEPTHS OF LESS THAN 55 FEET (17 METERS). Use of the sensors at greater depths s lkely to permanently damage the sensor membrane. WARNING: Ammonum, Ntrate, and Chlorde sensors should only be used n FRESHWATER. Setup - Ammonum, Ntrate, Chlorde Install the Ammonum, Ntrate, or Chlorde sensor n Port 2 f usng n conjuncton wth ph or ORP sensor on a or Quatro cable. See the Gettng Started Setup secton of ths manual for a complete lst of cable/sensor confguratons. Press Sensor, hghlght Setup, press enter. Hghlght ISE1 f usng an ammonum, ntrate, or chlorde sensor on a 60510, , or cable. If usng a or Quatro cable hghlght ISE1 f the sensor s nstalled n Port 1 or hghlght ISE2 f the sensor s nstalled n Port 2. Press enter

28 Cl - Chlorde NH4 - Ammonum NO3 - Ntrate Enabled allows you to enable or dsable the ISE functon and select whch ISE sensor s nstalled on the cable. Hghlght Enabled and press enter to enable (Q) or dsable (P) the ISE you selected prevously (ether ISE1 or ISE2). After enablng the ISE functon, choose the parameter you want enabled for that ISE. In ths example, NH4 s selected. If a sensor s Enabled that sn t connected to the nstrument, the dsplay wll show an unstable, false readng next to the unts. Dsplay - Ammonum, Ntrate, Chlorde Press Sensor, hghlght Dsplay, press enter. Hghlght ISE2(NH4), press enter. You wll not be able to Dsplay the sensor unless t s Enabled. ntrate or chlorde. Hghlght the value you wsh to dsplay and press enter to enable (Q). Ammonum can be dsplayed as NH4-N mg/l (Ammonum), NH3-N (Ammona) and/or NH4 mv (sensor sgnal). The same steps would be followed to dsplay Ammona s calculated from the ph, salnty, and temperature readngs. If a ph sensor s not n use, the nstrument wll assume the sample s neutral (ph 7) for the calculaton. If a conductvty sensor (Salnty) s not n use, the nstrument wll use the salnty correcton value entered n the Sensor Menu for the calculaton (see Salnty Correcton wthn the Dssolved Oxygen Setup secton of ths manual for more nformaton). Auto Stable - Ammonum, Ntrate, Chlorde Auto Stable ndcates when a readng s stable. When Auto Stable s enabled, AS wll blnk next to the parameter untl t s stable. Once the parameter s stable, AS wll stop blnkng To enable Auto Stable, press Sensor, hghlght Auto Stable and press enter. Hghlght ISE1 or ISE2 and press enter. Hghlght Enabled and/or Audo Enabled (nstrument wll beep when the stablty s acheved) and press enter to confrm. The Auto Stable Senstvty can be decreased or ncreased. Hghlght Senstvty and use the left and rght arrow keys to slde the bar. The more senstve you make t (larger black bar) the harder t s to acheve stablty n a changng envronment. The Auto Stable system works by examnng the prevous 5 readngs, computng the percent change n the data and comparng that change aganst a % threshold value. The % threshold value s determned by the Senstvty bar settng. The followng chart can be used as a gude when settng the Senstvty bar. Senstvty selected by User Most Senstve, Senstvty bar s set to the far rght % Data Varance Threshold 0.05% % % % 0 - Least Senstve, Senstvty bar s set to the far left 2.5% Wthn the Auto Stable menu, you can also choose to Hold All Readngs for as many parameters as you set for Auto Stable. For nstance, f ph and Ammonum have Auto Stable enabled and Hold All Readngs s also enabled, then the dsplay wll hold the readngs once ph and Ammonum have both reached ther Auto Stable settngs. You must press the Esc key to release the held dsplay n order to take subsequent readngs. Hold All Readngs must be reactvated after each use!

29 Calbraton - Ammonum, Ntrate, Chlorde The ISE/conductvty cable has a specalzed calbraton contaner that resembles a large test tube. Ths calbraton chamber can be used to calbrate the ISE sensors wth the conductvty sensor. A rng-stand should be used to support ths chamber. The ISE sensors can be calbrated at 1, 2, or 3-ponts. A 2-pont calbraton wthout chllng a thrd calbraton soluton s extremely accurate and s the preferred method. Greatest accuracy s acheved f the actual samples to be measured are wthn 10 C of the calbraton solutons. CALIBRATION TIP: Exposure to the hgh onc content of ph buffers can cause a sgnfcant, but temporary, drft n the ammonum, ntrate, and chlorde ISE sensors. Therefore, when calbratng the ph sensor, YSI recommends that you use one of the followng methods to mnmze errors n the subsequent readngs: When calbratng ph, remove ISE sensors from the cable bulkhead and plug the ports. After ph calbraton s complete, replace the ISE sensors and proceed wth ther calbraton wth no stablzaton delay. Calbrate ph frst, mmersng all of the sensors n the ph buffers. After calbratng ph, place the sensors n 100 mg/l ntrate or ammonum standard or 1000 mg/l chlorde standard dependng on the sensor n use and montor the readng. Usually, the readng starts low and may take awhle to reach a stable value. When t does, proceed wth the calbraton. Ths may take several hours. Preparng Chlorde Standards The followng recpes are provded for preparaton of 10 and 1000 mg/l chlorde reagents. Ntrate and Ammonum standards can be purchased from YSI or other laboratory supply companes. It s mportant to note that some of the chemcals requred for these solutons could be hazardous under some condtons. It s the responsblty of the user to obtan and study the MSDS for each chemcal and to follow the requred nstructons wth regard to handlng and dsposal of these chemcals. You wll need: Sold sodum chlorde or a certfed 1000 mg/l chlorde soluton from a suppler, magnesum sulfate, hgh purty water, a good qualty analytcal balance, 1000 ml volumetrc flask, an accurate 10 ml measurng devces, and 1000 ml glass or plastc storage vessels mg/l Standard: Accurately wegh grams of anhydrous sodum chlorde and transfer nto a 1000 ml volumetrc flask. Add 0.5 grams of anhydrous magnesum sulfate to the flask. Add 500 ml of water to the flask, swrl to dssolve all of the reagents, and then dlute to the volumetrc mark wth water. Mx well by repeated nverson and then transfer the 1000 mg/l standard to a storage bottle. Rnse the flask extensvely wth water pror to ts use n the preparaton of the 10 mg/l standard. Alternatvely, smply add 0.5 grams of magnesum sulfate to a lter of a 1000 mg/l chlorde standard from a certfed suppler. 10 mg/l Standard: Accurately measure 10 ml of the above 1000 mg/l standard soluton nto a 1000 ml volumetrc flask. Add 0.5 grams of anhydrous magnesum sulfate to the flask. Add 500 ml of water, swrl to dssolve the sold reagents, and then dlute to the volumetrc mark wth water. Mx well by repeated nverson and then transfer the 10 mg/l standard to a storage bottle. AMMONIUM (NH4+), NITRATE (NO3-), AND CHLORIDE CL- 2-POINT The calbraton procedures for ammonum, ntrate, or chlorde are smlar to ph. The only dfferences are the calbraton solutons. Recommended values for calbraton solutons and the order of calbraton are as follows: Sensor 1 st Pont 2 nd Pont Ammonum-ntrogen (NH4-N) 1 mg/l 100 mg/l Ntrate-ntrogen (NO3-N) 1 mg/l 100 mg/l Chlorde (Cl-) 10 mg/l 1000 mg/l Place the proper amount of 1 mg/l standard for Ammonum or Ntrate (10 mg/l for Chlorde) nto a clean, dry or pre-rnsed calbraton cup. Carefully mmerse the sensor nto the soluton. Allow at least 1 mnute for temperature equlbraton before proceedng. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. User ID wll appear automatcally. Select None f you do not want a User ID stored wth the calbraton. When enabled, these IDs are stored wth each calbraton record n the GLP fle

30 After selectng your User ID and/or Probe ID f approprate, hghlght Ammonum, Ntrate, or Chlorde to access the approprate calbraton, and press enter. The parameter you want to calbrate may appear under ISE1 or ISE2 dependng on your cable type and setup. The message lne wll show the nstrument s ready for the 1st calbraton pont. The nstrument wll dsplay the calbraton value at the top of the screen. If necessary, hghlght the Calbraton value and press enter to nput the correct value. Once the readngs stablze, hghlght Accept Calbraton and press enter to accept the frst calbraton pont. The message lne wll then dsplay Ready for pont 2. If you do not wsh to perform a second pont, press Cal to fnalze the calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select these felds and then press Cal to fnalze the calbraton. Alternatvely, you may press Esc to cancel the calbraton. To contnue wth the 2nd pont, rnse the sensor wth clean water, then dry t before placng t n the second calbraton standard. Allow at least 1 mnute for temperature equlbraton before proceedng. The nstrument wll dsplay the second calbraton value at the top of the screen. If necessary, hghlght the Calbraton value and press enter to nput the correct buffer value. Once the readngs stablze, hghlght Accept Calbraton and press enter to confrm the second calbraton pont. The message lne wll then dsplay Ready for pont 3 and you can contnue wth the 3rd calbraton pont f desred. If you do not wsh to perform a 3rd calbraton pont, press Cal to complete the calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select these felds and then press Cal to fnalze the calbraton. Alternatvely, you may press Esc to cancel the calbraton. AMMONIUM (NH4+), NITRATE (NO3-), AND CHLORIDE CL- 3-POINT A 2-pont calbraton wthout chllng a thrd calbraton soluton s extremely accurate and s the preferred method. If you must perform a 3-pont calbraton, the followng procedure requres one porton of the hgh concentraton calbraton soluton and two portons of the low concentraton calbraton soluton. The hgh concentraton soluton and one of the low concentraton solutons should be at ambent temperature. The other low concentraton soluton should be chlled to less than 10 C pror to calbraton. u WARNING: The chlled calbraton soluton MUST BE CHILLED TO AT LEAST 5 C COOLER THAN THE 1ST CALIBRATION POINT, otherwse the 1st pont wll be OVERRIDDEN. Follow the procedure for a 2-pont cal. After the second calbraton pont s complete, the message lne wth state Ready for pont 3. Place the proper amount of chlled 1 mg/l standard (10 mg/l for the chlorde) nto a clean, dry or pre-rnsed calbraton cup. Carefully mmerse the sensor nto the soluton. Allow for temperature equlbraton. If necessary, hghlght Calbraton value and press enter to manually enter the 3rd buffer value. Once the readngs are stable, hghlght Accept Calbraton and press enter to confrm. Press Cal to complete the calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select these felds and then press Cal to fnalze the calbraton. Alternatvely, press Esc to cancel the calbraton. Takng Measurements To obtan the most accurate readngs, be sure the nstrument s calbrated before takng measurements. Dssolved Oxygen Turn the nstrument on and wat 5-15 mnutes f usng a polarographc sensor. If usng a feld cable/sensor, nstall the sensor guard to protect the sensor and membrane. Place the probe n the sample to be measured and gve the probe a quck shake to release any ar bubbles. Allow the temperature readngs to stablze. Next, str the probe n the sample to overcome the strrng dependence of the dssolved oxygen sensor. You must provde at least 3 nches per second for 2.0 PE membranes, 6 nches per second for 1.25 PE membranes, and 12 nches per second for Teflon membranes. Once the values plateau and stablze, you may record the measurement and/or log the data set. The dssolved oxygen readng wll drop over tme f strrng s ceased. If placng the DO sensor nto a stream or fast flowng waters t s best to place t perpendcular to the flow and NOT facng nto the flow. If usng the DO sensor n an aeraton tank/basn, t s helpful to make sure bubbles do not burst on the membrane snce ths may cause unstable readngs. You should be able to prevent ths by pontng the sensor upwards so t s facng 50 51

31 the sky and then twst tyng, zp tyng, or rubber bandng the bulkhead to the cable. Makng a smple curve to the cable wthout bendng or breakng the cable wll allow you to lower the sensor nto the aeraton tank whle the sensor ponts skyward so the bubbles are no longer burstng on the membrane surface. Conductvty The conductvty sensor wll provde quck readngs as long as the entre sensor s submerged and no ar bubbles are trapped n the sensor area. Immerse the probe nto the sample so the sensors are completely submerged and then shake the probe to release any ar bubbles. Occasonal cleanng of the sensor may be necessary to mantan accuracy and ncrease the responsveness. To clean the sensor, use the conductvty cleanng brush wth a mld detergent. ph/orp ph and ORP readngs are typcally quck and accurate. However, t may take the sensors a lttle longer to stablze f they become coated or fouled. To mprove the response tme of a sensor, follow the cleanng steps n the Mantenance secton of ths manual. Durng a contnuous log, the Start Loggng dalog box on the Run screen wll change to Stop Loggng. Press Enter to stop contnuous loggng. Fles and Ste Lsts Fle Memory To vew the fle memory, press Fle. The Data Memory shows a percentage ndcatng the amount of memory avalable. If the fle memory s near 0%, fles should be downloaded to a PC and/or deleted to free up memory. Ammonum, Ntrate, and Chlorde These sensors may take a lttle longer to stablze f the tps are drty or fouled. If nstalled wth a ph sensor, always mantan a clean ph sensor for a more rapd sensor stablzaton. Vewng Saved Data These sensors can only be used n freshwater. Loggng Data Press Fle, hghlght Vew Data and press enter. Confgurng your data vew: Log One Sample s already hghlghted n Run mode. Press enter to open a submenu. If Use Ste Lst and or Use Folder Lst are enabled n the Loggng Setup menu, you wll have to opton to select these two tems before the data pont s logged. If necessary, use the keypad to create a new Ste or Folder name. If Ste Lst and Folder Lst are dsabled n the System menu, you wll not see these optons when loggng a sample. Once the Ste and/or Folder name s selected, hghlght Log Now and press Enter. The nstrument wll confrm that the data pont was successfully logged. If you would lke to log at a specfc nterval vs. loggng one sample at a tme or vce versa, press System, then hghlght Loggng and press enter. Select Contnuous Mode and adjust the tme Interval f necessary. On the Run screen, the opton to log wll change from Log One Sample to Start Loggng based on the tme nterval entered n the Loggng Menu. Ste: wll allow you to vew data from one partcular ste or all stes. Hghlght Ste, press enter, and select the ste you wsh to vew data from or select All Stes to vew data from all stes. Folder: wll allow you to vew data from one partcular folder or all folders. Hghlght Folder, press enter, and select the fle you wsh to vew data from or select All Folders to vew data from all folders. Begn Date, Begn Tme, End Date, and End Tme: wll allow you to vew data collected between a specfc tme perod. Hghlght the 52 53

32 Ste Lst tme qualfer you would lke to set, press enter, and use the numerc entry screen to select the date/tme you wsh to vew. After makng your selectons n the Data Flter, hghlght Show Data and press enter. The data wll have date and tme stamps. You wll lkely have to scroll up and down and sde to sde usng the arrow keys to completely vew the data fle. No more than 100 data records can be vewed at one tme. To modfy the Ste Lst, press Fle, hghlght Ste Lst, and press enter. Enter new ste names or edt exstng stes wth the alpha/numerc entry screen. Ste lsts can also be created and edted on your PC wth Data Manager and then downloaded to the nstrument. D a t a M a n a g e r Desktop Software Data Manager s provded wth the purchase of a Pro Plus Instrument. Data Manager s a powerful Wndows based software that wll allow you to easly manage logged data, set up nstruments, and conduct real tme studes. Mnmum PC system requrements for Data Manager are Wndows 2000 wth SP4 (mnmum) or Wndows XP wth SP2 (mnmum) Operatng System, 300 MHz or hgher Pentum -compatble CPU, 128 MB of RAM or hgher, 80 MB or more of free hard-dsk space, USB 2.0, and Mcrosoft.NET. Data Manager needs to be nstalled on a PC before use and before you try to connect a Pro Plus to your PC. Frst nstall Data Manger, then connect the communcatons saddle to the PC and, lastly, connect the saddle to your Pro Plus. Data Manager wll dentfy the connected nstruments by ther Unt ID.Refer to the Data Manager Readme fle for detaled nstallaton nstructons. Data Manager wll then recognze the attached nstruments. From the home screen of Data Manager, see below, you can select one of the followng functons: Retreve Instrument Data, Real Tme Instrument Data, Instrument Confguraton, or Vew Saved Fle/Data. Folder To modfy the Folder Lst, press Fle, hghlght Folder Lst, and press enter. Enter new Folder names or edt exstng folders wth the alpha/numerc entry screen. Delete Data Press Fle, hghlght Delete Data, and press enter. Enter the crtera for the data you wsh to delete n the Delete Data Flter, then hghlght Delete Data and press enter

33 Usng the Communcatons Saddle u WARNING: DO NOT connect the Communcatons Saddle to your PC before nstallng Data Manager. The Communcaton Saddle drvers must be nstalled pror to connectng t to your PC. The drvers wll nstall automatcally durng the Data Manager nstallaton. The frst tme the saddle s connected to the PC, you may have to walk through a couple of nstallaton wzards. For detaled nstructon, please refer to the Readme fle located on the CD that was ncluded wth your nstrument. A PC wll recognze the Communcatons Saddle (saddle) as a YSI water qualty nstrument wth or wthout the Pro Plus nstalled n the saddle. To connect the saddle to a Pro Plus, smply algn the saddle to the oval secton on top of the nstrument and push t down to snap t n place (Fgure 6). Manage Logged Data Data that has been logged to the Professonal Plus can easly be uploaded to the PC va the provded USB saddle. You can upload sensor data, GLP fles, ste lsts, and nstrument confguraton fles ndvdually or all at once. After connectng the nstrument to the PC va the USB saddle and cable and launchng Data Manager, clck the Retreve Instrument Data tab. Clck on the Instrument s Unt ID you would lke to retreve data from, then select the fles you would lke to retreve and clck Start. Once the sensor data s uploaded to the PC, you can graph and vew tabular data by nstrument Unt ID, date/tme, ste name, and/or folder name. Ths allows you to confgure the report accordng to your needs. You can choose to vew all data from all nstruments, or select a certan date/tme range for only a few specfc nstruments, there are multple ways to vew the data. Once the report has been defned, you wll be able to prnt the graph and/or export the table. Data Manager takes nformaton management one step further and allows you to delete specfc ponts nstead of entre fles. Ths allows you to clean up data that s no longer needed or that may have been collected erroneously, for example, when the sensor was out of the water. If you can not delete data due to regulaton and complance purposes, Data Manager has the soluton. Whle vewng logged data or real tme data, you have the ablty to tag ndvdual data ponts wth comments. Fgure 6. Locate the oval algnment groove at the top of the nstrument and nsde the saddle. Insert the saddle nto ths oval groove. Press the saddle towards the back of the nstrument untl t snaps nto place. Connect the USB cable to the top of the saddle and to a USB port on the PC. Once Data Manager s launched, the program wll recognze all saddles wth nstruments connected to the PC. The nstrument wll be powered through the saddle and USB connecton when connected to the PC. However, the nstrument must stll have batteres nstalled n order to keep the date and tme correct when powerng the PC off at nght. Make sure the nstrument s turned off frst, then turn off the PC to keep the nstrument from runnng all nght on the batteres. If you power t off and power off the PC the nstrument wll keep the correct date and tme f t has batteres nstalled. If batteres are not nstalled, the nstrument s date and tme wll not reman correct and wll need to be reset each mornng. In addton to sensor data, you wll be able to vew GLP fles, ste lsts, and confguraton fles that have been uploaded from the nstrument. These can be prnted and exported as well. Real Tme Studes Data Manager allows you to vew real tme data on the PC. After selectng your nstrument, clck the Real Tme Instrument Data tab. Next, nput your sample nterval, ste/folder name, select the parameters you wsh to vew and clck OK. You must clck Start on the next screen to begn your real tme study. Choose to hde the table or graph by uncheckng the box next to these optons. Clck Stop, then Edt Setup to change the Y-scale mn/max of the graph, to select dfferent colors, or to name your graph. Add a comment to a data pont by clckng n the comment feld of the table next to the data pont. You may also Prnt the graph and Export the data for vewng n another program

34 Confgure Instruments Data Manager allows for easy and quck confguraton of sngle or multple nstruments. Once you have uploaded a ste lst or confguraton fle, you can edt t as needed, save t, and download t to other nstruments. You no longer need to confgure each nstrument ndvdually. By usng the same confguraton fle for all nstruments, you can rest assured that all nstruments wll have dentcal settngs. New ste lsts and confguraton fles can be created n Data Manager as well. These lsts and fles can be downloaded to one or multple nstruments. Save tme by creatng these fles on your PC and downloadng them to the nstrument as opposed to creatng them on the nstrument. C a r e, M a n t e n a n c e, and Storage Ths secton descrbes the proper procedures for care, mantenance and storage of the sensors. The goal s to maxmze ther lfetme and mnmze down-tme assocated wth mproper sensor usage. Updatng Instrument Frmware The nstrument s frmware can be updated va There you wll fnd the new frmware fle and nstructons on how to update the nstrument. There s no need to send the nstrument back to the factory for upgrades. General Mantenance General Mantenance - O-rngs The nstrument utlzes o-rngs as seals to prevent water from enterng the battery compartment and sensor ports. Followng the recommended procedures wll help keep your nstrument functonng properly. The same nspecton should be made of the o-rngs assocated wth the sensor connectors when they are removed. If no drt or damage to the o-rngs s evdent, then they should be lghtly greased wthout removal from ther groove. However, f there s any ndcaton of damage, the o-rng should be replaced wth an dentcal o-rng. At the tme of o-rng replacement, the entre o-rng assembly should be cleaned. To remove the o-rngs: Use a small, flat-bladed screwdrver or smlar blunt-tpped tool to remove the o-rng from ts groove. Check the o-rng and the groove for any excess grease or contamnaton. If contamnaton s evdent, clean the o-rng and nearby plastc parts wth lens cleanng tssue or equvalent lnt-free cloth. Alcohol can be used to clean the plastc parts, but use only water and mld detergent on the o-rng tself. Also, nspect the o-rngs for ncks and mperfectons. u Usng alcohol on o-rngs may cause a loss of elastcty and may promote crackng. Do not use a sharp object to remove the o-rngs. Damage to the o-rng or the groove may result. Before re-nstallng the o-rngs, make sure to use a clean workspace, clean hands, and avod contact wth anythng that may leave fbers on the o-rng or grooves. Even a very small amount of contamnaton (har, grt, etc.) may cause a leak. To re-nstall the o-rngs: Place a small amount of o-rng grease between your thumb and ndex fnger. (More grease s NOT BETTER!) Draw the o-rng through the grease whle pressng the fngers together to place a very lght coverng of grease to the o-rng. Place the o-rng nto ts groove makng sure that t does not twst or roll. Use your grease-coated fnger to once agan lghtly go over the matng surface of the o-rng. If the o-rngs and sealng surfaces are not mantaned properly, t s possble that water can enter the battery compartment and/or sensor ports of the nstrument. If water enters these areas, t can severely damage the battery termnals or sensor ports causng loss of battery power, false readngs, and corroson to the sensors or battery termnals. Therefore, when the battery compartment ld s removed, the o-rng that provdes the seal should be carefully nspected for contamnaton (e.g. debrs, grt, etc.) and cleaned f necessary. u Do not over-grease the o-rngs. The excess grease may collect grt partcles that can compromse the seal. Excess grease can also cause the waterproofng capabltes of the o-rng to dmnsh, potentally causng leaks. If excess grease s present, remove t usng a lens cloth or lnt-free cloth

35 General Mantenance - Sensor Ports It s mportant that the entre sensor connector end be dry when nstallng, removng or replacng. Ths wll prevent water from enterng the port. Once a sensor s removed, examne the connector nsde the port. If any mosture s present, use compressed ar to completely dry the connector or place drectly n front of a steady flow of fresh ar. If the connector s corroded, return the cable to your dealer or drectly to an YSI Repar Center. Remove sensors upsde down (facng the ground) to help prevent water from enterng the port upon removal. Sensor Mantenance Sensor Mantenance - Dssolved Oxygen Membrane Cap Installaton The DO sensor (Polarographc and Galvanc) s shpped wth a dry, protectve red cap that wll need to be removed before usng. Remove the protectve cap or used membrane cap and replace t wth a new membrane cap followng these nstructons: Remove the sensor guard to access the sensor tp. Unscrew and remove any old membrane cap by holdng the sensor when unscrewng the membrane cap and dscard. Thoroughly rnse the sensor tp wth dstlled or DI water. Fll a new membrane cap wth O2 sensor electrolyte soluton that has been prepared accordng to the drectons on the bottle. Be very careful not to touch the membrane surface. Lghtly tap the sde of the membrane cap to release bubbles that may be trapped. Thread the membrane cap onto the sensor. It s normal for a small amount of electrolyte to overflow. Polarographc Sensors - Model # The KCl (potassum chlorde) soluton and the membrane cap should be changed at least once every 30 days durng regular use. In addton, the KCl soluton and membrane should be changed f (a) bubbles are vsble under the membrane; (b) sgnfcant deposts of dred electrolyte are vsble on the membrane; and (c) f the sensor shows unstable readngs or other sensor-related symptoms. Durng membrane changes, examne the gold cathode at the tp of the sensor and the slver anode along the shaft of the sensor. If ether the slver anode s black n color or the gold cathode s dull, the sensor may need resurfaced usng the fne sandng dsks ncluded n the membrane kt. Do not sand the electrode every membrane change as ths s not routne mantenance. In fact, vsually, the anode may appear tarnshed and operate just fne. YSI recommends usng the 400 grt wet/dry sandng dsks to resurface the electrodes f the sensor has dffculty stablzng or calbratng after a membrane change. To resurface the sensor usng the fne sandng dsk, follow the nstructons below. Gold Cathode: For correct sensor operaton, the gold cathode must be textured properly. It can become tarnshed or plated wth slver after extended use. Never use chemcals or abrasves not recommended or suppled by YSI. Frst dry the sensor tp completely wth lens cleanng tssue. Wet a sandng dsk wth a small amount of clean water and place t face up n the palm of your hand. Next, wth your free hand, hold the sensor n a vertcal poston, tp down. Place the sensor tp drectly down on the sandng dsk and twst t n a crcular moton to sand the gold cathode. The goal s to sand off any buld-up and to lghtly scratch the cathode to provde a larger surface area for the O2 soluton under the membrane. Usually, 3 to 4 twsts of the sandng dsk are suffcent to remove deposts and for the gold to appear to have a matte fnsh. Rnse thoroughly and wpe the gold cathode wth a wet paper towel before puttng on a new membrane 60 61

36 cap. If the cathode remans tarnshed, contact YSI Techncal Support or the Authorzed dealer where you purchased the nstrument. Slver Anode After extended use, a thck layer of Slver Chlorde (AgCl) bulds up on the slver anode reducng the senstvty of the sensor. The anode must be cleaned to remove ths layer and restore proper performance. The cleanng can be chemcal or mechancal: Chemcal cleanng: Remove the membrane cap and rnse the electrodes wth deonzed or dstlled water. Soak the sensng anode secton of the sensor n a 14% ammonum hydroxde soluton for 2 to 3 mnutes or n a 3% ammona soluton overnght for 8-12 hours (most household ammona cleaners are typcally around 3%). Rnse heavly n cool tap water followed by a thorough rnsng wth dstlled or deonzed water. The anode should then be thoroughly wped wth a wet paper towel to remove the resdual layer from the anode. You can smell the tp of the sensor to help ensure all the ammona has been rnsed off. Trappng resdual ammona under the new membrane cap can quckly tarnsh the electrode and/or gve false readngs. Chemcal cleanng should be performed as nfrequently as possble. Frst attempt a membrane change and recalbrate. If a new membrane does not resolve the problem, then proceed wth cleanng. Mechancal cleanng: In order to sand the slver anode along the shaft of the sensor, smply hold the sensor n a vertcal poston. Wet the sandng dsk wth a small amount of clean water then gently wrap t around the sensor shaft and twst t a few tmes to lghtly sand the anode (the goal s to smply sand off any buld-up wthout scratchng or removng layers of the anode tself). Usually, 3 to 4 twsts of the sandng dsk are suffcent to remove deposts. However, n extreme cases, more sandng may be requred to regenerate the orgnal slver surface. After completng the sandng procedure, repeatedly rnse the electrode wth clean water and wpe wth lens cleanng tssue to remove any grt left by the sandng dsk. Thoroughly rnse the entre tp of the sensor wth dstlled or deonzed water and nstall a new membrane. u IMPORTANT: Be sure to: (1) Use only the fne sandng dsks provded and (2) Sand as mentoned n the above procedures. Not adherng to ether of these nstructons can damage the electrodes. If ths procedure s unsuccessful, as ndcated by mproper electrode performance, contact YSI Techncal Support or the Authorzed dealer where you purchased the nstrument. Galvanc Sensors Model # We recommend that the Sodum Chlorde (NaCl) soluton and the membrane cap be changed at least once every 60 days durng regular use. In addton, the NaCl soluton and membrane should be changed f (a) bubbles are vsble under the membrane; (b) sgnfcant deposts of dred electrolyte are vsble around the membrane; and (c) f the sensor shows unstable readngs or other sensor-related symptoms. The Galvanc dssolved oxygen sensor s contnuously reducng oxygen even when the dsplay of the nstrument s not actve. Ths factor allows the sensor to be used wth no warm-up perod as soon as the nstrument s powered on (nstant on DO). However, because the sensor s on all the tme, some sold from the oxdaton of the znc anode wll form n the electrolyte wthn 1-2 weeks of actvaton. Small amounts of the sold wll generally cause no performance problems, but excessve amounts may result n jumpy dssolved oxygen readngs. The rate of sold formaton s dependent on the type of membrane nstalled. The formaton of solds based on membrane type typcally form more rapdly wth the 5912 (1 ml Teflon), less rapd wth 5913 (1.25 ml PE), and least rapd wth 5914 (2 ml PE). u The Galvanc DO sensor soluton wll appear mlky whte after use but wll NOT affect the accuracy of the sensor unless there s excessve buld up. The color change s acceptable and normal as long as DO readngs reman stable. At the tme the membrane cap s changed, YSI recommends that you rnse the anode (slver shaft of the sensor) wth purfed water and wpe wth a clean paper towel. If whte deposts are evdent on the anode after cleanng, YSI recommends that you remove ths materal by sandng the anode wth the sandpaper dsk enclosed n your membrane kt. Follow the Mechancal Cleanng nstructons under the Polarographc Slver Anode secton. IMPORTANT: Be sure to: (1) Use only the fne sandng dsks provded and (2) Sand as mentoned n the above procedures. Not adherng to ether of these nstructons can damage the electrodes. WARNING: DO NOT PERFORM THE POLAROGRAPHIC CHEMICAL CLEANING ON A GALVANIC SENSOR. If ths procedure s unsuccessful, as ndcated by mproper electrode performance, contact YSI Techncal Support or the Authorzed dealer where you purchased the nstrument

37 Sensor Mantenance - Conductvty The openngs that allow sample access to the conductvty electrodes should be cleaned regularly. The small cleanng brush ncluded n the Mantenance Kt s deal for ths purpose. Dp the brush n clean water and nsert t nto each hole 10 to 12 tmes. In the event that deposts have formed on the electrodes, t may be necessary to use a mld detergent (laboratory grade soap or bathroom foamng tle cleaner) wth the brush. Rnse thoroughly wth clean water, then check the response and accuracy of the conductvty cell wth a calbraton standard. If ths procedure s unsuccessful, as ndcated by mproper electrode performance, contact YSI Techncal Support or the Authorzed dealer where you purchased the nstrument. Sensor Mantenance - Temperature You must keep the temperature porton of the sensor free of buld up. Other than that, the sensor requres no mantenance. The conductvty cleanng brush can be used to scrub the temperature sensor f needed. Alternatvely, you can use a toothbrush to clean the sensor. Sensor Mantenance - ph, ORP and Combnaton ph/orp Typcal workng lfe for ph and ORP sensors s approxmately months dependng on usage, storage, and mantenance. Proper storage and mantenance generally extends the sensor s workng lfe. Cleanng s requred whenever deposts or contamnants appear on the glass and/or platnum surfaces or when the sensor s response slows. The cleanng can be chemcal and/or mechancal. Removng the sensor from the cable may make cleanng easer. Intally, use clean water and a soft clean cloth, lens cleanng tssue, or cotton swab to remove all foregn materal from the glass bulb and/or platnum button. Then use a mostened cotton swab to carefully remove any materal that may be blockng the reference electrode juncton of the sensor. u CAUTION: When usng a cotton swab, be careful NOT to wedge the swab between the guard and the glass sensor. If necessary, remove cotton from the swab tp, so that the cotton can reach all parts of the sensor tp wthout stress. You can also use a ppe cleaner for ths operaton f more convenent. If good ph and/or ORP response s not restored, perform the followng addtonal procedure: 1. Soak the sensor for mnutes n clean water contanng a few drops of commercal dshwashng lqud. 2. GENTLY clean the glass bulb and platnum button by rubbng wth a cotton swab soaked n the cleanng soluton. 3. Rnse the sensor n clean water, wpe wth a cotton swab saturated wth clean water, and then rernse wth clean water. If good ph and/or ORP response s stll not restored, perform the followng addtonal procedure: 1. Soak the sensor for mnutes n one molar (1 M) hydrochlorc acd (HCl). Ths reagent can be purchased from most lab supply dstrbutors. Be sure to follow the safety nstructons ncluded wth the acd. 2. Rnse the sensor n clean water, wpe wth a cotton swab saturated wth clean water (not DI water), and then rernse wth clean water. To be certan that all traces of the acd are removed from the sensor crevces, soak the sensor n clean water for about an hour wth occasonal strrng. If bologcal contamnaton of the reference juncton s suspected or f good response s not restored by the above procedures, perform the followng addtonal cleanng step: 1. Soak the sensor for approxmately 1 hour n a 1:1 dluton of commercally-avalable chlorne bleach. 2. Rnse the sensor wth clean water and then soak for at least 1 hour n clean water wth occasonal strrng to remove resdual bleach from the juncton. (If possble, soak the sensor for a perod of tme longer than 1 hour n order to be certan that all traces of chlorne bleach are removed.) Then rernse the sensor wth clean water and retest. Dry the port and sensor connector wth compressed ar and apply a very thn coat of o-rng lubrcant to all o-rngs before renstallaton. Sensor Mantenance - Chlorde Typcal workng lfe for chlorde sensors s approxmately 3-6 months dependng on usage, storage, and mantenance. Proper storage and mantenance generally extends the sensor s workng lfe. The chlorde sensor s consdered a pellet membrane ISE. As always, when handlng sensors, care should be taken to avod damagng the membrane. Ths 64 65

38 sensor can be regenerated by washng wth alcohol and/or gently polshng wth fne emery paper n a crcular moton to remove any deposts or dscoloraton, then thoroughly washng wth deonzed water to remove any debrs. The sensor may requre soakng n the hgh standard chlorde calbraton soluton to recover ts performance. Sensor Mantenance - Ammonum and Ntrate Typcal workng lfe for ammonum and ntrate sensors s approxmately 3-6 months dependng on usage, storage and mantenance. Proper storage and mantenance generally extends the sensor s workng lfe. The ammonum and ntrate sensors are PVC membranes. As always, when handlng a sensor, care should be taken to avod damagng the membrane. After extensve use the membranes may become coated wth a depost or scoured wth fne scratches whch may cause a slow or reduced response (low slope) or unstable readngs. Deposts may be removed wth a fne jet of deonzed water or rnsng n alcohol followed by soakng n the hgh standard calbraton soluton. Gently dab dry wth a lnt-free tssue before takng measurements. Sensor Storage Short-term Storage The cable assembly s suppled wth a sensor storage contaner, or sleeve, that attaches to the cable. The contaner s used for short-term storage (less than 30 days). Be sure to keep a small amount of mosture (tap water) n the contaner durng storage. Ths s done to mantan a 100% saturated ar envronment whch s deal for short-term sensor storage. The sensors should not be submersed n water. The ntent s to create a humd ar storage envronment. Long-term Storage Long-term Storage - Temperature No specal storage s requred. The temperature sensor can be stored dry or wet as long as solutons n contact wth the thermstor are not corrosve (for example, chlorne bleach). Long-term Storage Temperature: -5 to 70 C (23 to 158 F) Long-term Storage - Conductvty No specal storage s requred. Sensors can be stored dry or wet as long as solutons n contact wth conductvty electrodes are not corrosve (for example, chlorne bleach). However, t s recommended that the sensor be cleaned wth the provded brush pror to and after long term storage. Long-term Storage Temperature: -5 to 70 C (23 to 158 F) Long-term Storage - Dssolved Oxygen Dssolved oxygen sensors (Polarographc and Galvanc) should be stored n a dry state for long term storage Frst, remove the membrane cap and thoroughly rnse the sensor wth clean water. Next, ether blow t dry wth compressed ar or allow to ar dry completely. Install a clean, dry new membrane cap over the sensor to keep t dry and to protect the electrodes. After storng the sensor for a long perod of tme, t s necessary to condton the sensor by puttng a new membrane wth electrolyte soluton on the sensor and then turnng the nstrument on to allow the sensor suffcent tme to stablze. Long-term Storage Temperature: -5 to 70 C (23 to 158 F) Long-term Storage - ph The key to ph sensor storage, short or long-term, s to make certan that the sensor does not dry out. Sensors whch have been allowed to dry out due to mproper storage procedures may be rreparably damaged by the dehydraton and wll requre replacement. You can try to rehydrate the sensor by soakng t (preferably overnght) n a potassum chlorde soluton or a ph 4 buffer before attemptng to calbrate. To store the sensor, remove t from the cable and seal the vacant port wth a port plug. Fll the orgnal shppng/storage vessel (plastc boot or bottle) wth buffer 4 soluton and then submerge the sensor nto the soluton. The sensor should reman submerged n the soluton durng the storage perod; therefore, make certan that the vessel s sealed to prevent evaporaton and perodcally check the vessel to ensure the sensor does not dry out. Long-term Storage Temperature: 0 to 30 C (32 to 86 F) It s mportant not to store the ph sensor n dstlled or deonzed water as the glass sensor may be damaged by exposure to ths medum

39 Long-term Storage - ORP To store, remove the sensor from the cable and seal the vacant port wth the provded plug. Fll the orgnal shppng/storage vessel (plastc boot or bottle) wth buffer 4 soluton and then submerge the sensor nto the soluton. The sensor should reman submerged n the soluton durng the storage perod; therefore, make certan that the vessel s sealed to prevent evaporaton and perodcally check the vessel to ensure the sensor does not dry out. Long-term Storage Temperature: 0 to 30 C (32 to 86 F) Long-term Storage - Ammonum, Ntrate, and Chlorde The key to ISE sensor storage, short or long-term, s to make certan that the sensor does not dry out. Sensor junctons that have been allowed to dry out due to mproper storage procedures may be rreparably damaged by the dehydraton and wll requre replacement. You can attempt to rehydrate the sensor by soakng t (preferably overnght) n the sensor s hgh calbraton soluton before attemptng to calbrate. The recommended storage of these sensors s n most ar. Remove the sensor from the cable and seal the vacant port wth the provded plug. Place the sensor n ts orgnal shppng storage vessel (plastc boot or bottle) wth a small amount of tap water or ts hgh calbraton standard. The vessel should reman a saturated ar envronment. The sensor only needs to be kept n most ar, not submerged. Make certan that the vessel s sealed to prevent evaporaton. Long-term Storage Temperature: 0 to 30 C (32 to 86 F) Troubleshootng Illegal Value may appear durng alpha/numerc entry on the message lne. Ths only appears f the values entered do not match the formattng. Ths wll also appear n GLP securty area f the password s ncorrect. Error Messages If readngs for a certan parameter are over range you wll see a seres of and f the readngs are under range you wll see a seres of plus the error message along the bottom of the screen. If you see a seres of????? that wll ndcate that a certan parameter can not be calculated. The followng are potental error messages: Probe Temp over range Probe Temp under range Case Temp over range Case Temp under range ph over range ph under range ORP over range ORP under range Cl over range Cl under range NH4 over range NH4 under range NO3 over range NO3 under range DO over range DO under range Conductvty over range Conductvty under range Barometer over range Barometer under range Error messages for the sensors typcally ndcate a need to properly clean the sensor. Frst verfy the sensor s properly setup n the Sensor menu, then conduct the recommended cleanng and attempt to calbrate the sensor. If ths does not work, t may ndcate the useful lfe of the sensor has been reached and may need to be replaced. You may also contact Techncal Support to help determne the next step. If you forget the GLP Securty Password please contact YSI Tech Support at [email protected], , or Help Durng use of the Professonal Plus nstrument, press Queston screen to vew help messages drectly on the dsplay. from any 68 69

40 Dssolved Oxygen The dssolved oxygen sensors wll use Probe Current (DO ua) and Probe Slope (%/ua) as part of ther GLP fle records. The followng nformaton ndcates the acceptable values for each of these readngs: Polarographc DO at 25 º C, 100% saturated ar envronment at 760 mmhg Probe Current 1.25 ml PE membrane Average 6.15 ua (mn ua, max ua) 2.0 ml PE membrane Average 3.38 ua (mn ua, max ua) 1 ml Teflon membrane Average ua (mn ua, max ua) Probe Slope 1.25 ml PE membrane Average % sat/ua (mn ua, max ua) 2.0 ml PE membrane Average % sat/ua (mn ua, max ua) 1 ml Teflon membrane Average 6.14 % sat/ua (mn ua, max ua) Restore Default C a l b r a t o n Va l u e s Occasonally, the nstrument may need to have the factory calbraton default values restored. In order to accomplsh ths press Calbrate, hghlght Restore Default Cal and press enter. Hghlght the parameter you wsh to restore to default and press enter. Next you wll be asked to confrm the operaton. Hghlght Yes and press enter to confrm. Accessores / Part Numbers Cable Part Number* Descrpton Professonal Plus Instrument , -4, -10, -20, or -30 1, 4, 10, 20, or 30-meter cable for ISE/temp , -4, -10, -20, or -30** 1, 4, 10, 20, or 30-meter cable for DO/temp , 4, -10, -20, or -30 1, 4, 10, 20 or 30-meter cable for Cond/temp , 4, -10, -20, or -30 1, 4, 10, 20, or 30-meter cable for ISE/ISE/temp , -4, -10, -20, or -30 1, 4, 10, 20, or 30-meter cable for ISE/DO/ temp , 4, -10, -20, or -30 1, 4, 10, 20, or 30-meter cable for ISE/Cond/ temp , -4, -10, -20, or -30 1, 4, 10, 20 or 30-meter cable for DO/Cond/ temp , -4, -10, -20, or -30 1, 4, 10, 20 or 30-meter Quatro cable for DO/ Cond/temp/ISE/ISE meter ph/temp sngle juncton lab-grade combo electrode meter ph/temp sngle juncton lab-grade combo electrode meter ORP/temp sngle juncton lab-grade combo electrode meter ORP/temp sngle juncton lab-grade combo electrode meter ph/orp/temp sngle juncton labgrade combo electrode meter ph/orp/temp sngle juncton labgrade combo electrode Sensor Part Number Descrpton Galvanc DO sensor Polarographc DO sensor ph (ISE) ORP (ISE) *** ph/orp Combnaton (ISE) **** Ammonum (ISE) 70 71

41 Sensor Part Number **** Chlorde (ISE) **** Ntrate (ISE) Descrpton Self-Strrng BOD sensor Conductvty/Temperature sensor for Quatro cable * All cables nclude temperature. Cables wth conductvty nclude sensor (no need to order separate conductvty sensor). ** Specal order cables up to 100-meters are avalable wth cables. *** Not compatble wth X or Quatro cables. **** Freshwater only Accessory Part Number Descrpton Flow cell, standard, 203 ml (for two-port sensors) Accessory Part Number Descrpton Flow cell kt for Quatro cable assembles Flow cell mountng spke Communcatons saddle kt Data Manager desktop software Carryng case, soft-sded Flow cell kt, 1 or 2 port sensor (ncludes flow cell for two-port sensors wth the adapter for one-port sensors) Carryng case, hard-sded Flow cell adapter, sngle port (use wth flow cell to accommodate one-port sensors) Mantenance kt Brush, tube cleaner Grease, o-rng 72 73

42 Accessory Part Number Belt clp Ultra clamp Trpod clamp Descrpton Solutons Part Number Descrpton ,000 us/cm conductvty soluton (quart) ,000 us/cm conductvty soluton (quart) ,000 us/cm conductvty soluton (8 pnts) 3682 Zobell ORP soluton (125 ml) 3824 ph 4, 7, 10 buffers (2 pnts of each) mg/l ammonum soluton (500 ml) mg/l ammonum soluton (500 ml) mg/l ammonum soluton (500 ml) mg/l ntrate soluton (500 ml) mg/l ntrate soluton (500 ml) mg/l ntrate soluton (500 ml) 5580 Confdence Soluton (verfes ph, ORP, conductvty sensor performance) Declaraton of Conformty Cable management kt Weght, sensor/cable, 4.9 oz Weght, sensor/cable, 24 oz., Weght, sensor/cable, 51 oz., Shoulder strap The undersgned hereby declares on behalf of the named manufacturer under our sole responsblty that the lsted product conforms to the requrements for the lsted European Councl Drectve(s) and carres the CE mark accordngly. Manufacturer: YSI Incorporated 1725 Brannum Lane Yellow Sprngs, OH USA Product Name: Professonal Plus Water Qualty Instrument Model Numbers Instrument/Accessory: Professonal Plus ( ) / ProComm (605604) Probe/Cable Assembles: , , , , , , , 60510, 60520, 60530, , , , , Sensors: , , , , , , , , , Conforms to the followng: Drectves: EMC 2004/108/EC RoHS 2002/95/EC WEEE 2002/96/EC 74 75

43 Harmonzed Standards: Supplementary Informaton: Authorzed EU Representatve EN :2006, Electrcal equpment for measurement, control, and laboratory use EMC requrements Part 1: General Requrements EN :2006, Electrcal equpment for measurement, control and laboratory use EMC requrements Part 2-3: Partcular Requrements Test confguraton, operatonal condtons, and performance crtera for transducers wth ntegrated or remote sgnal condtonng. EN :2006, Electromagnetc compatblty (EMC) Part 3-2: Lmts Lmts for harmonc current emssons (equpment nput current < 16A per phase). EN :1995 +A1:2001 +A2:2005, Electromagnetc compatblty (EMC) Part 3: Lmts Secton 3: Lmtaton of voltage fluctuatons and flcker n low-voltage supply systems for equpment wth rated current < 16A. All performance met the contnuous unmontored operaton crtera as follows: 1. ESD, EN , Performance Crteron B 2. Radated Immunty, EN , Performance Crteron A 3. EFT, EN , (EFT) Performance Crteron B 4. Surge, EN , Performance Crteron B 5. Conducted Immunty, EN , Performance Crteron A 6. Voltage Interrupts, EN , Performance Crteron B 7. RF Emssons, EN55011:1998, A1:1999 Class B equpment YSI Hydrodata Ltd Unt 8, Busness Centre West, Avenue 1 Letchworth, Hertfordshre, SG6 2HB UK The undersgned hereby declares on behalf of the named manufacturer under our sole responsblty that the lsted product conforms to the requrements for electrcal equpment under US FCC Part 15 and ICES-003 for unntentonal radators. Manufacturer: YSI Incorporated 1725 Brannum Lane Yellow Sprngs, OH USA Product Name: Model Numbers Instrument/Accessory: Professonal Plus ( ) / ProComm (605604) Probe/Cable Assembles: , , , , , , , 60510, 60520, 60530, , , , , Sensors: , , , , , , , , , Conforms to the followng: Standards: FCC 47 CFR Part , Subpart B, Class B, Rado Frequency Devces ICES-003:2004, Dgtal Apparatus Supplementary Informaton: Tested usng ANSI C (excludng sectons 4.1, 5.2, 5.7, 9, and 14) Sgned: Lsa M. Abel Date: 22 February 2008 Ttle: Drector of Qualty Sgned: Lsa M. Abel Date: 22 February 2008 Ttle: Drector of Qualty 76 77

44 The undersgned hereby declares on behalf of the named manufacturer under our sole responsblty that the lsted product conforms wth the Australan and New Zealand Electromagnetc Compatblty (EMC) requrements for generc products to be used n resdental, commercal, and lght ndustral envronments. Manufacturer: YSI Incorporated 1725 Brannum Lane Yellow Sprngs, OH USA Product Name: Professonal Plus Water Qualty Instrument Model Numbers Instrument/Accessory: Professonal Plus ( ) / ProComm (605604) Probe/Cable Assembles: , , , , , , , 60510, 60520, 60530, , , , , Sensors: , , , , , , , , , Conforms to the followng: Standards: AS/NZS :1999, Electromagnetc Compatblty (EMC) Generc emsson standard Part 1: Resdental, commercal, and lght ndustry. Sgned: Lsa M. Abel Date: 22 February 2008 Ttle: Drector of Qualty Recyclng YSI s commtted to reducng the envronmental footprnt n the course of dong busness. Even though materals reducton s the ultmate goal, we know there must be a concerted effort to responsbly deal wth materals after they ve served a long, productve lfe-cycle. YSI s recyclng program ensures that old equpment s processed n an envronmentally frendly way, reducng the amount of materals gong to landflls. Prnted Crcut Boards are sent to facltes that process and reclam as much materal for recyclng as possble. Plastcs enter a materal recyclng process and are not ncnerated or sent to landflls. Batteres are removed and sent to battery recyclers for dedcated metals. When the tme comes for you to recycle, follow the easy steps outlned at Contact Informaton Orderng and Techncal Support Telephone: Fax: Emal: Mal: Internet: (US) (Globally) Monday through Frday, 8:00 AM to 5:00 ET (orders) (techncal support) [email protected] or [email protected] YSI Incorporated 1725 Brannum Lane Yellow Sprngs, OH USA When placng an order please have the followng avalable: 1.) YSI account number (f avalable) 2.) Name and phone number 3.) Purchase Order or Credt Card 4.) Model Number or bref descrpton 5.) Bllng and shppng addresses 6.) Quantty Telephone: (US) Servce Informaton YSI has authorzed servce centers throughout the Unted States and Internatonally. For the nearest servce center nformaton, please vst www. ys.com and clck Support or contact YSI Techncal Support drectly at When returnng a product for servce, nclude the Product Return form wth cleanng certfcaton. The form must be completely flled out for a YSI Servce Center to accept the nstrument for servce. The form may be downloaded from by clckng on the Support tab, then the Product Return Form button

45 Item # Rev D Drawng # A March YSI Incorporated.