Programme PhD / Master course Advanced Microscopy and Vital Imaging 2014. Time Item Location 09.00 09.15 Welcome and brief overview of the course



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Programme PhD / Master course Advanced Microscopy and Vital Imaging 2014 Monday, June 2, 2014 09.00 09.15 Welcome and brief overview of the course 09.15 10.00 Basics of Microscopy 10.15 12.45 Practical course: Bright field microscopy, a hands-on course GROUP I UNS 40 C.4.577 /Helma Kuijpers [] Maastricht University Medical Center 10.15 11.15 Fluorescence microscopy GROUP II 13.30 14.30 Fluorescence microscopy GROUP I 13.30 16.00 Practical course: Bright field microscopy, a hands-on course GROUP II UNS 40 C.4.577 /Helma Kuijpers [] Maastricht University Medical Center

Tuesday, June 3, 2014 09.00 10.00 Principles of confocal scanning laser microscopy Edwin Lamers Carl Zeiss Benelux 10.00 11.00 Live cell imaging using GFP-technology in combination with CSLM 11.00 12.00 Sample preparation GROUPS 5-8 14.00 15.00 Sample preparation 11.00 14.00 Demonstrations (according to schedule, 45 min per group) Confocal scanning laser microscopy / Helma Kuijpers Dept. Molecular Cell Biology, Maastricht University Medical Center 14.00 17.00 Demonstrations (according to schedule, 45 min per group) GROUPS 5-8 G.5.223A Confocal scanning laser microscopy / Helma Kuijpers Dept. Molecular Cell Biology, Maastricht University Medical Center G.5.223A

Wednesday, June 4, 2014 09.00 09.45 Analysis of the neuromuscular junction quantitative microscopy Mario Losen Dept. of Basic Neurosciences, Maastricht University Medical Center 09.45 10.30 Backgrounds and applications of STED Microscopy Patrick van Wieringen Leica Microsystems BV 10.30 10.45 Coffee/tea break 10.45 11.30 Two-photon laser microscopy for the three-dimensional study of tissues 11.45 13.15 ImageJ GROUPS 5-7 15.00 16.30 ImageJ 11.45 17.00 Demonstrations (according to schedule, 45 min per group) GROUPS 1-7 Two-photon scanning laser microscopy 0f Pc ruimte Of pc ruimte H.3.347 Timo Rademakers [Zhuojun Wu] Sanquin, Amsterdam

Thursday, June 5, 2014 09.00 10.00 Advances in multiphoton microscopy in vivo imaging in cardiovascular research Timo Rademakers Sanquin, Amsterdam 10.00 11.00 High throughput and high content cellular analysis for systems biology Winnok De Vos Ghent University 11.00 11.15 Coffee/tea break 11.15 12.15 Advanced Electron Microscopy Peter Peters EM-Unit and Dept. Molecular Cell Biology, Maastricht University Medical Center 12.30 Departure to FEI Nanoport Company, Eindhoven Peter Peters /Helma Kuijpers EM-Unit and Dept. Molecular Cell Biology, Maastricht University Medical Center 14.15 15.00 Welcome and introduction biological application of electron microscopy (by bus) Wim Busing Sr Applications Specialist NanoBiology, FEI Company, Eindhoven 15.00 16.30 Demo's on Quanta 3D FEG (SEM), Quanta 250 (SEM), and Tecnai 20 (TEM) Wim Busing Sr Applications Specialist Nano Biology,FEI Company, Eindhoven 16.45 Departure back to Maastricht University Medical Center FEI, Eindhoven (by bus)

Maastricht Microscopy Meeting (M 3 ) Friday, June 6, 2014 Symposium Advanced Microscopy and Vital Imaging Greepzaal, level 4, azm Maastricht University Medical Center, P. Debyelaan 25, Maastricht 09.30 10.00 Arrival & Coffee 10.00 10.10 Welcome by Dept. of Molecular Cell Biology, Maastricht University Medical Center 10.10 10.55 Speaker 1 10.55 11.40 Speaker 2 xx 11.40 12.30 Speaker 3 xx 12.30 13.45 Lunch 13.45 14.30 Speaker 4 Dept. ofxxxxxx 14.30 15.15 Speaker 5 x 15.15 16.00 Speaker 6 16.00-16.30 Speaker 7 16.30 17.30 Closure & Drinks Participation is free, but please note that registration is obligatory. You can register by sending an e-mail, including your name and department, to the secretary of Molecular Cell Biology, Maastricht University Medical Center. Email secr-mcb@maastrichtuniversity.nl Telephone +31-43-3881351