PROGRAMMA / PROGRAM. Moleculaire Biologie en Cytometrie Cursus Mei 2013, SCK CEN, Mol
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1 Moleculaire Biologie en Cytometrie Cursus Mei 2013, SCK CEN, Mol PROGRAMMA / PROGRAM Molecular Biology and Cytometry Course 16 th -17 th May 2013, SCK CEN, Mol MB&C course MOL 2013 programma / program p1/5
2 Programma Donderdag 16 mei 2013 Program Thursday May 16 th u00 09u30 Welkom met koffie / Welcome coffee 09u30 09u50 Official Welcome 09u50 11u00 Plenary lecture 1: Overview of techniques in imaging, molecular biology and flowcytometry (Winnok De Vos, Friedel Nollet and Vincent van der Velden) 11u00 11u20 Koffiepauze / Coffee break 11u20 11u50 Parallel lecture 2a (clinical): Flow cytometry settings, pitfalls and artefacts (Pieter De Schouwer) Parallel lecture 2b (research): Advanced Proteomics (Tobias Corne) 11u50 12u20 Parallel lecture 3a (clinical): Lymphoma diagnosis using flow cytometry on lymph node biopsies (Johan Willemse) Parallel lecture 3b (research): Label free imaging (Andre Skirtach) 12u20 12u50 Parallel lecture 4a (clinical): Minimal residual disease in AML (Vincent van der Velden) Parallel lecture 4b (research): Genome Editing (Tom Sieprat) 12u50 13u30 Middagmaal / Lunch 13u30 15u00 Workshop reeks/session D1 15u00 15u30 Koffiepauze / Coffee break 15u30 16u00 Workshop reeks/session D2 16u00 16u30 Round table and discussion 16u30 17u30 Reception and drinks MB&C course MOL 2013 programma / program p2/5
3 Programma Vrijdag 17 mei 2013 Program Friday May 17 th u00 09u30 Welkom met koffie / Welcome coffee 09u30 10u00 Parallel lecture 5a (clinical): Real time PCR settings, pitfalls and artefacts (Sabine Franke) Parallel lecture 5b (research): Bio informatics in 2013 (Raphael Kiekens) 10u00 10u30 Parallel lecture 6a (clinical): Minimal residual disease in pediatric ALL using Ig/TCR targets (Marleen Bakkus) Parallel lecture 6b (research): Characterization of the Immune Microenvironment in Breast Cancer using Flow (Karen Willard-Gallo) 10u30 11u00 Parallel lecture 7a (clinical): Molecular tests for choosing targeted therapy in oncology (Brigitte Maes) Parallel lecture 7b (research): Correlative Microscopy (FEI: Diane van Rossum) 11u00 11u20 Koffiepauze / Coffee break 11u20 11u50 Planary lecture 8: PCR chips and miniature flow cytometry (Tim Stakenborg) 11u50 13u00 Plenary lecture 9: Future techniques in imaging, molecular biology and flowcytometry (Winnok De Vos, Friedel Nollet and Jan Philippé) 13u00 13u45 Middagmaal / Lunch 13u45 15u15 Workshop reeks/session V1 15u15 15u45 Koffiepauze / Coffee break 15u45 16u15 Workshop reeks/session V2 16u15 16u45 Round table and discussion 16u45 18u00 Reception and farewell drinks MB&C course MOL 2013 programma / program p3/5
4 Onderwerpen van de workshops: Workshop topics: Research & Basic techniques in Microscopy 101 Opticals and basic microscope settings (Louis de Saint-Georges/Giuseppe Pani) 102 Image informatics (Winnok De Vos) 103 Label free imaging (Winnok De Vos) 104 Correlative Microscopy (Diane van Rossum, FEI) 105 Visit to FEI Company Nanoport, Eindhoven NL (by bus on Friday afternoon) Research & Basic techniques in Flowcytometry 201 Set up, calibratie en compensatie van een flowcytometer (Roland Langelaar, BD) 202 Cell sorting (Roland Langelaar, BD) 203 Microparticles and platelets (Francois Mullier) 204 Een blik in de toekomst: PCR chips, miniature flowcytometrie en de nieuwste sequencing platformen (Tim Stakenborg) Research & Basic techniques in Molecular Biology 301 DNA and RNA extraction from different tissues (Arlette Michaux & Hussein el- Saghire & Nathalie Vanhoudt & Elke Boone) 302 PCR and primer development and troubleshooting (Walter Verstrepen) 303 Validatie van PCR gebaseerde assays (Lieselotte Cnops) 304 Multiplex Array Assay and Microarray (Rafi Benotmane & Michael Beck) 305 Bio-informatics (Raphael Kiekens) 306 Next generation sequencing (Pieter Monsieurs) 307 Apoptosis and DNA repair (Khalil Abouelaradat) 308 Multiplex Array Assay & Intracytoplasmic and cytokine multicolour protocols (Hanane Derradji & Myriam Ghardi) Microscopy Hematology 401 Dysplasia in the diagnosis of MDS (Bernard Husson) Flowcytometry Hematology 501 Euroflow standardisatie (Johan Willemse & Sofie Goovaerts) 502 Multicolor protocols and de validatie hiervan (Dennis Tielemans, BD) 503 MRD analyse in AML (Vincent van der Velden) 504 MRD analyse in CLPD en Myeloom (Jan Philippé) 505 High sensitivity PNH flowcytometrie analyse (Tine Venken) MB&C course MOL 2013 programma / program p4/5
5 506 Flow cytometric screening and immune monitoring in primary immune deficiencies (Melissa Dullaers) 507 Harmonisation of multicolor settings and Leukaemia/Lymphoma approach (Antoine Pacheco, Analis) 508 Infinicyt (Dennis Tielemans, BD) Molecular Biology Hematology and Pathology 601 Interpretatie van klonaliteitsanalyse, IGH and TCRG (Elke Boone) 602 Quantitative standardized BCRABL analysis (Hakim El Housni) 604 Diagnostische en follow-up moleculaire markers in AML (Friedel Nollet) 605 Fragment analyse, sequencing en IGH hypermutatie analysis (Sabine Franke) 606 Chimerisme en DNA fingerprinting (to be announced) 607 FISH en PCR in moleculaire pathologie inclusief BRAF/KRAS/EGFR/ALK/HER2 (Femke Hillen) 608 High Resolution Melting (HRM) analysis (Karl Vandepoele) MB&C course MOL 2013 programma / program p5/5
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