cdna Clones, Vectors and Gene Synthesis

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1 cdna Cloes, Vectors ad Gee Sythesis Your complete soultios for molecular biology eeds ORF Cloes TrueCloe TrueORF PrecisioShuttle TrueORF Gold No-protei Codig Cloes mirna plasmids 3'-UTR reporter plasmids shrna plasmids Gee Sythesis: Cloe by Desig Ay variat Ay species Ay vector PO Box 520 HA9 7YN, UK Tel: (0) Fax: (0) Web:

2 OriGee Cloe Offerig Overview Comprehesive. Quality. Fast Delivery. Whe you eed a DNA cloe, be it a cdna cloe or a cloe of o-codig sequece, OriGee is your best choice. Our 100,000 ready-to-ship cdna cloes offer a quick ad cost-savig solutio. With the acquisitio of Blue Hero, the leadig gee sythesis compay, OriGee is ehaced with the proficiecy to create customized DNA cloes of ay species ad ay desig. cdna cloes for protei expressio Cloes with o-codig sequeces TrueCloe (utagged) TrueORF (tagged) Gee sythesis Gee sythesis mirna plasmids 3'-UTR reporter plasmids Mai utility Protei Tagged protei Customize Customize mirna over Target validatio Expressio i expressio expressio for mirna ative form Expressio host Mammalia Mammalia Shuttle to 60 vectors Customize Customize Mammalia mammalia Species Huma/Mouse Huma/Mouse Ay species, Ay species, Huma/mouse Huma ay sequece ay sequece HOW TO FIND A CLONE Search: A search box is located at the top of every page of the OriGee website ad the followig terms ca be used. NCBI Accessio Number (eg. NM_000044) Gee symbol of the gee (eg. VEGF) Gee ame (Caspase 8) Cloe descriptio (eg. adroge receptor, or kiase deficiet mutat) Browse: O the cloe collectio page, cda/cloe_set.aspx, gees ca also be browsed by a gee family or a pathway. A few popular categories are listed below as examples. Protei Kiase Wt Pathway Phosphotase Agiogeesis GPCR Notch Pathway Secreted Tumor Metastasis

3 Why Should You Choose OriGee s cdna Cloes? Quality! Selectio! Delivery! FEATURES Comprehesive: Geome wide coverage for huma ad mouse ad gee sythesis available Versatile: utagged or tagged cloes (>60 taggig optios) Expressio validatio (See page 6) Trasfectio-ready DNA: Plasmids are purified with io-exchage colums. Pathway Focused cdna Cloe Sets (See page 9) Other Providers: Cloes arrive as bacterial stock Start Here Or OriGee: Cloes arrive as purified plasmid DNA Start Here Day 1 Platig for sigle coloies Day 2 Ioculate for bacterial expasio Day 3 Plasmid isolatio ad verificatio Day 4 Start your experimets For protei-codig ORF sequeces, there are two type of cloes TrueCloes: Library-based, full-legth cdna cloes that usually cotai ative 5' ad 3' utraslated regios; i a CMV mammalia expressio vector TrueORF Cloes: Tagged ope readig frame (ORF) cloes i OriGee's CMV based PrecisioShuttle etry vector. The ORF isert ca be easily shuttled with a simple digestio/ligatio reactio ito a wide variety of tagged destiatio vectors. For o-protei codig sequeces, OriGee offers mirna expressio cloe (See page 10) shrna cloes (See RNAi brochure orwww.origee.com/rnai/) 3'-UTR reporter cloes (See page 11)

4 TrueORF Myc/DDK- or GFP-tagged ORF cloes for coveiet applicatio The TrueORF product lie is the ew geeratio of cdna cloe products. Ulike TrueCloes, a TrueORF cloe eables expressio of the ecoded trascript as a tagged protei. This facilitates multiple dowstream applicatios that utilize a ati-tag atibody such as: Protei detectio, protei purificatio, subcellular localizatio, ad others. TrueORF VECTORS SV40 ori f1 ori CMV promoter Amp r f1 ori CMV promoter Neo r /Ka r pcmv6-etry 4.9 kb Kozak Sgf I Asc I Hid III ColE1 pcmv6-ac-gfp 6.6 kb Kozak Sgf I Asc I Hid III PolyA ColE1 PolyA Myc DDK Rsr II Mlu I Not I Xho I Neo r PolyA sigal SV40 ori GFP Pme I Rsr II Mlu I Not I Xho I All TrueORF iserts are housed i either pcmv6-etry or pcmv6-ac-gfp vector. Both vectors allow for easy shuttlig by a simple 'cut-ad-paste' mechaism ito ay of the PrecisioShuttle Destiatio Vectors (see page 6). A TrueORF cloe expresses the ecoded sequece as a C-termially Myc ad DDK-tagged protei or a GFPtagged protei. DDK is the same as FLAG. FLAG is a registered trademark of Sigma-Aldrich. Sgf I ORF MIu I G C G A T C G C * C A T G / / N N N A C G C G T Kozac Cosesus EcoR I BamH I Kp I RBS Sgf I C T A T A G G G C G G C C G G G A A T T C G T C G A C T G G A T C C G G T A C C G A G G A G A T C T G C C G C C G C G A T C G C C A T G ORF Mlu I Not I Xho I Myc.-Tag * N N N ACG CGT ACG CGG CCG CTC GAG CAG A A A CTC ATC TCT GA A GAG T R T R R L E Q K L I S E E EcoR V DKK-Tag Pme I Fse I G A T C T G G C A G C A A A T G A T A T C C T G G A T T A C A A G G A T G A C G A C G A T A A G G T T T A A A C G G C C G G C C G C D L A A N D I L D Y K D D D D K V Stop * The last codo before the Stop codo of the ORF The diagram above is applicable to the majority of huma ORFs that do ot have iteral Sgf I ad Mlu I sites. Other rare restrictio sites i the MCS are utilized for ORFs with iteral sites for the two ezymes.

5 TrueORFS ARE EXCELLENT FOR Mammalia overexpressio of tagged proteis (over 60 combiatio of tags available) Detectio of the exogeously itroduced protei Purificatio of the overexpressed protei Protei iteractio ad localizatio studies (e.g. orgaelle markers) Autophagosome RC10005 Cellular imagig of the exogeously itroduced protei Tagged protei expressio i a cell-free system (eg. TNT) Cytoskeleto RC N-Tag(s) C-Tag(s) TrueORF ADVANTAGES His-HA-BLK His-BLK Myc-Flag-BLK His-Flag-BLK Myc-Flag-BTK GFP HA-BLK Myc-Flag-BTK His-HA-BLK Coveiece: TrueORFs provide a istat solutio for tagged protei expressio Flexibility: TrueORF ca be shuttled ito multiple destiatio vectors. Accuracy: TrueORFs have verified ad guarateed isert sequeces Prove: TrueORFs have bee rigorously tested for expressio of the target proteis ad their tags. Ati-His Tag (TA ) Ati-HA Tag (TA ) Ati-Myc Tag (TA ) Ati-DDK Tag (TA ) Ati-BTK Ati-BLK So far 12,000 TrueORF have passed the protei expressio validatio. The umber is expected to icrease to 20,000 i the ear future. Wester blot aalysis of HEK293 cell lysate over-expressio BLK or BTK tagged with with idicated epitopes.

6 PrecisioShuttle System Tagged protei expressio made simple To accommodate diverse taggig eeds, OriGee devised the ovel PrecisioShuttle system to allow easy subcloig of a ORF from oe tagged vector to aother. The TrueORF Etry Vector cotais C-termial tags of Myc ad DDK. A large pael of destiatio vectors are available so you ca express a ORF with differet tags or with tags at differet eds of the protei. The key i the PrecisioShuttle system is the utilizatio of two rarecuttig restrictio edoucleases, Sgf I ad Mlu I. PRECISIONSHUTTLE VS. RECOMBINATION SHUTTLE SYSTEM (E.G. GATEWAY SYSTEM): SCHEMATIC OF THE PRECISION SHUTTLING SYSTEM PrecisioShuttle Fuctioal etry vector No subcloig eeded Restrictio ezymes-based Low cost No IP restrictio Iserts>18Kb are readily shuttled Gatway is a registered trademark of Life Techology. Gateway Etry vector NOT for expressio Subcloig eeded Recombiatio-based Expesive Coditioal licesig Ustable plasmid whe isert>5kb 2 aa liker appeded >10aa liker appeded Neo r /Ka r Digestio with Sgf I ad Mlu I Neo r /Ka r Sgf I TrueORF Etry-Vector Sgf I TrueORF Etry-Vector Amp r Protei ORF Neo r Mlu I Myc-DDK Tag Protei ORF Mlu I Amp r Amp r Neo r Neo r Ligatio Precisio Shuttle Destiatio- Vector Tag Sgf I Mlu I Digestio with Sgf I, Mlu I ad CIP Tag Sgf I Precisio Mlu I Shuttle Destiatio- Vector Trasformatio ad Selectio with Ampicilli Tag Sgf I Protei ORF Precisio Shuttle Mlu I Destiatio- Vector

7 PrecisioShuttle Vectors Etry ad Destiatio PrecisioShuttle Etry Vector Cell Selectio PrecisioShuttle Destiatio Vector Mammilia Selectio pcmv6-etry pcmv6-ac-rfp (C-termial Myc ad DDK Tagged, -R) pcmv6-an-yfp pcmv6-ac-yfp pcmv6-ac-fp602 PrecisioShuttle Destiatio Vector Mammilia Selectio pcmv6-ac-fp635 pcmv6-ac-his pcmv6-ac-mkate pcmv6-ac-myc pcmv6-ac-mgfp pcmv6-ac-ha pcmv6-ac-mrfp pcmv6-ac-ddk pcmv6-ac-myfp pcmv6-ac-myc-his pcmv6-ac-mbfp pcmv6-ac-myc-ddk pcmv6-ac-mcfp pcmv6-ac-ha-his pcmv6-an-fp602 pcmv6-ac-ddk-his pcmv6-an-fp635 pcmv6-ac-gfp pcmv6-an-mkate pcmv6-an-his pcmv6-an-mgfp pcmv6-an-myc pcmv6-an-mrfp pcmv6-an-ha pcmv6-an-myfp pcmv6-an-ddk pcmv6-an-mbfp pcmv6-an-his-myc pcmv6-an-mcfp pcmv6-an-myc-ddk pcmv6-ac-fc pcmv6-an-his-ha pcmv6-ac-fc-s pcmv6-an-his-ddk pcmv6-an-fc pcmv6-an-gfp pcmv6-an-fc-s pcmv6-ac pcmv6-ac-3ddk ptune Iducible pcmv6-an-3ddk pcmv6-a-bsd Blasticidi pcmv6-ac-ires-gfp-puro Puromyci pcmv6-a-em7-bsd Blasticidi ptune-gfp pcmv6-a-hygro Hygromyci pcmv6-an-gfp-c-his pcmv6-a-puro Puromyci pcmv6-a-gfp pcmv6-ac-ires-gfp pex-n-his-gst pex-n-gst pex-n-his UNDERSTAND A VECTOR BY ITS NAME p CMV6 - A C - RFP pex-c-his pex-1 Expressio Promoter Selectio i E. Coli Termial Tag(s) pcmv6-an-rfp

8 TrueORF Gold Cloes Expressio-validated cdna Cloes TESTED INDIVIDUALLY BY WESTERN BLOT Each TrueORF Gold cloe has bee used to trasfect huma cell lies for protei expressio. OriGee has produced over 12,000 over-expressio lysates from TrueORFtrasfected HEK293 cells ad subsequetly purified over 5,000 huma proteis from those cells. SEQUENCE VERIFIED Each TrueORF Gold cloe is fully sequeced with dowloadable chromatogram files posted olie. No more worryig about mutatios, deletios, or frameshift whe usig TrueORF Gold HEK293 were trasfected with L) empty vector R) TrueORF for Myc/DDK-tagged htert(cat# RC217436). The lysates were aalyzed usig ati-ddk atibody to show over-expressio of htert TRANSFECTION READY No eed for subcloig. No eed for plasmid preparatio. TrueORF Gold cloes are costructed with all elemets (promoter, polyadeylatio sigal, drug-selectio marker, etc) for protei-expressio i mammalia cells. EASILY SHUTTLED INTO 60 VECTORS OriGee has prepared over 60 shuttle vectors with matchig cloig sites for easy trasfer of the isert usig PrecisioShuttle, a simple cut-ad-paste procedure. Fluorescet-protei taggig vectors (27) Iducible vectors (2) Epitope-taggig vectors (23) Bacterial expressio vectors (5) Selectio markers (8) NEXT-DAY SHIPMENT

9 TrueCloe Authetic full-legth cdna cloes for expressio ad fuctioal studies of a ative protei OriGee s TrueCloe covers both huma ad mouse species. The vectors for the two species differ slightly. Amp r pcmv6-xl4/5/ bp ColE1 f1 ori CMV promoter SV40 ori MCS PolyA sigal Sac I T7/SP6 Promoter Not I EcoR I Bgl II Kp II EcoRV Hid III Sal I Xba I Not I Sma I f1 ori SV40 ori pcmv6-ka/neo 4906 bp Ka r /Neo r HSV TK PolyA CMV promoter ColE1 MCS PolyA sigal T7 Promoter Sac I EcoR I Hid III Sma I All TrueCloes are uidirectioally cloed i the EcoR I ad Sal I restrictio sites. The Sal I site is destroyed durig cloig, ad caot be reused. The isert ca be liberated by a simple digestio with Not I. All mouse TrueCloes are cloed uidirectioally betwee two sites i the MCS. Please cotact OriGee s techical support professioals for details. TrueCLONES ARE EXCELLENT FOR Overexpressio of the ative protei i mammalia cells Fuctioal studies of ative protei Templates for quatitative PCR Probes for hybridizatio-based detectio, such as Norther blots or FISH assays Protei expressio i cell-free systems (eg. TNT) TrueCLONE ADVANTAGES Cost-effective ad time-savig alterative to de ovo cloig Expressio-ready ad trasfectio-ready Authetic cdnas represetig ative trascripts Cosistet vector system facilitates high-throughput screeig

10 Pathway-focused cdna Cloe Sets For researchers who wish to obtai a gee family- or a pathway-focused cdna cloe collectio for either high throughput screeig or for archivig. OriGee offers the easy optio of cloe set. The major beefit of purchasig a cloe set is the major discout depedig o the size of the collectio. PRE-MADE CLONE SETS Cat # Descriptio Price (subject to chage) TCAC105 Trasmembrae Set (3,890) $300K TCAC106 Access Set 10,000 (10,000) $250K TCAC107 Access Protei Kiase Set (315) $25K TCAC108 Access Phosphatase Set (137) $15K TCAC109 Access GPCR Set (215) $20K CLONE SETS FOR CUSTOMIZED ASSEMBLY OriGee s website lists over 100 commoly studied pathways, gee families ad research focuses so that the customer ca assemble their ow cloe collectio of iterest with ease. Sample collectios Cloe Sets TrueORF TrueCloe (Myc-DDK Tagged) (Utagged) GPCR Kiase-deficiet mutat N/A 337 Protei Kiase Secreted Trasmembrae Agiogeesis Cloe Sets TrueORF TrueCloe (Myc-DDK Tagged) (Utagged) Apoptosis Breast Cacer Cytokies Huma Stem Cell Huma Tumor Metastasis Notch Wt Pathway More o website

11 MicroRNA Expressio Plasmids Comprehesive coverage for huma ad mouse geome OriGee provides cloes for over-expressio of microrna of your choice. OriGee s MicroRNA precursor cotais pre-micorna (60-70t) with ts up- ad dow-stream of flakig sequece. It was amplified from huma geomic DNA ad cloed ito OriGee s pcmv6-mir Vector. Upo trasfectio, the cellular machiery will process the CMV-drive expressio of mirna precursor ito mature mirna ad cellular fuctio ca be aalyzed. FEATURES & BENEFITS Pri-miRNA Geome wide mirna coverage 652 huma ad 486 mouse Sequece cofirmatio of the precursor microrna mgppp Drosha AAAAAA GFP for trasfectio moitorig Drosha cut selectio for stable cell establishmet GFP trasfectio of microrna expressio plasmids i HEK293 cells Pre-miRNA 5 3 Dicer cut Dicer Mir205 Mir143 Mir34b Mature mirna 5 3 Empty Vector No-trasfected mirna EXPRESSION PLASMIDS Sold idividually as 10ug trasfectio-ready DNA or ca be purchased as followig sets RISC complex Catalog No. SC SC SC SC Descriptio Mouse mirna expressio plasmid set (486 vectors, 10ug each i 2-D bar coded tubes) Huma mirna expressio plasmid set (652 vectors, 10ug each i 2-D bar coded tubes) Mouse mirna expressio plasmid set (486 vectors, 2ug each i 96-well plates) Huma mirna expressio plasmid set (652 vectors, 2ug each i 96-well plates)

12 3'-UTR Reporter Cloes for mirna Target Validatio Luciferase reporter assays for huma geome The 3'-UTR plasmids provide quatitative assessmet of the ihibitory effect betwee mirnas ad their potetial target gees. The 3'-UTR sequece of a gee was cloed dowstream of the firefly luciferase gee. The chimeric trascript level is regulated by its iteractio with mirna(s), which results i varied luciferase activity quatifiable by a colorometric assay. MicroRNAs ad their 3'-UTR targets ca be assayed for edogeous microrna activity or for overexpressed microrna i a co-trasfectio experimet. SV40 P STOP STOP NEO IRES Luciferase 3'-UTR Target SV40 P STOP STOP NEO IRES Luciferase INTERACTION IRES-Luc Traslatio microrna RISC complex NO INTERACTION IRES-Luc Traslatio microrna RISC complex 3'-UTR Target AAAAAA 3'-UTR Target AAAAAA LOW LEVEL LUC TRANSLATION Luciferase LUC TRANSLATION Luciferase Iteractio betwee mirna ad UTR. Reduced luciferase expressio No iteractio betwee mirna ad UTR. No effect o luciferase expressio FEATURES & BENEFITS Geome wide coverage (>20,000 huma gee) Firefly luciferase as the easy-to-assay reporter RFP for trasfectio moitorig High sesitivity from IRES-drive traslatio of the expressio cassette SV40 F1 Ori CMV RFP Neo r / Ka r IRES pmirtarget Vector 7.9 kb PolyA ColE1 Luciferase PolyA EcoR I Sgf I Asc I Rsr II Mlu I Not I Xba I pcmv - Mir + Mir205 target pcmv - Mir205 + Mir205 target OriGee has used a ew desig adapted from C.P.Peterse et al. 2006, to dramatically icrease the sesitivity of detectio by decreasig the 3'UTRluciferase reporter expressio to a very low level.

13 Orgaelle Marker Plasmids Label subcellular structures with a simple trasfectio PRODUCT DESCRIPTION GFP- or RFP-tagged huma cdna cloes for orgaelle-specific markig Idividually validated by cofocal microscopy for correct labelig Eable labelig of live or fixed cells without chemical or atibodies APPLICATIONS Direct orgaelle markig Moitorig of protei traffickig Study orgaelle morphology ad dyamics Protei co-localizatio with orgaelles Autophagosome MAP1LC3A MAP1LC3B DiRas3 ATG12 Cetrosome PLK1 Microtubule TUBA1B Membrae Proteis LCK Mitochodria BID PDHA1 Coated Pit CLTB Lysosome LAMP1 Peroxisome PXMP2 Other Cytoskeleto Proteis MAP-EB3 PFN1 : RFP-tagged marker : GFP-tagged marker Golgi Apparatus B4GALT1 TGOLN Nucleus LMNB1 CCND1 Edosome Rab4 Rab5 RhoB Edoplasmic Reticulum CALR Focal Adheasio Fiber FAK VCL ZYX Neuroal Markers GAP43 SYP Acti Stress Fibers ACTB Fractio trackig durig erichmet or purificatio $480 each, provided as 10ug purified trasfectio-ready plasmids. Cat# Orgaelle Symbol RC100004(G)/RC100036(R) Autophagosome ATG12 RC100011(G)/RC100043(R) Autophagosome Di-Ras3 RC100020(G)/RC100052(R) Autophagosome MAP1LC3A RC100021(G)/RC100053(R) Autophagosome MAP1LC3B RC100023(G)/RC100055(R) Cetrosome PLK1 RC100010(G)/RC100042(R) Coated pit CLTB RC100002(G)/RC100034(R) Cytoskeleto ACTB RC100019(G)/RC100051(R) Cytoskeleto MAP-EB3 RC100022(G)/RC100054(R) Cytoskeleto PFN1 RC100030(G)/RC100062(R) Cytoskeleto TUBA1B RC100008(G)/RC100040(R) Edoplasmic reticulum CALR RC100025(G)/RC100057(R) Edosome Rab4 RC100026(G)/RC100058(R) Edosome Rab5 Cat# Orgaelle Symbol RC100031(G)/RC100063(R) Focal adheri fiber VCL RC100032(G)/RC100064(R) Focal adheri fiber ZYX RC100005(G)/RC100037(R) Golgi apparatus B4GalT1 RC100029(G)/RC100061(R) Golgi apparatus TGOLN RC100016(G)/RC100048(R) Lysosome LAMP1 RC100006(G)/RC100038(R) Mitochodria PDHA1 RC100007(G)/RC100039(R) Mitochodria BID RC100013(G)/RC100045(R) Neuroal axis GAP43 RC100009(G)/RC100041(R) Nucleus CCND1 RC100018(G)/RC100050(R) Nucleus LMNB1 RC100024(G)/RC100056(R) Peroxisome PXMP2 RC100017(G)/RC100049(R) Plasma membrae LCK RC100028(G)/RC100060(R) Syaptic vesicles SYP RC100027(G)/RC100059(R) Edosome RhoB RC100012(G)/RC100044(R) Focal adheri fiber FAK

14 Gee Sythesis from Blue Hero OriGee acquired Blue Hero, the gee sythesis expert, to be able to offer a complete solutio for gee research. Blue Hero has bee the gold stadard i gee sythesis sice Its superior techology ad outstadig quality are demostrated i a series of iovative achievemets. The first compay to sythesize a 52KB gee (2007) The primary supplier for the 1st bacterial geome (2008) The sole DNA source for the 1st sythetic cell by J. Craig Veter Ist. (2010) With Blue Hero's capabilities, OriGee becomes a oe-stop shop for all of your cdna eeds. Our combied stregths provides our customers with broader coverage, quicker delivery ad better service. We are 100% committed to servig you with high quality stadards ad improved busiess efficiecy uder strict cofidetiality. WHY TRUST BLUE HERON WITH YOUR GENE SYNTHESIS PROJECT? Get Ay Gee Our proprietary techology ca sythesize more challegig gees tha ay other vedor. Fast Most gees are delivered withi 2-4 weeks. Guarateed Quality Each gee will match the exact sequece you order. Secure ad Cofidetial Order ad commuicate via a secure, dedicated website. No Claim to Your Ivetios You ow full right ad title to the products delivered ad retai IP rights. Optioal Sequece Desig Free codo optimizatio to covert AA sequece ito DNA sequece for best protei expressio.

15 ORDER AND SYNTHESIS OVERVIEW Optioal-codo optimizatio of amio acid sequece Submit DNA sequece GeeMaker: sythesizes, cloes ad sequece verifies the order Purified plasmid DNA ad bacteria cotaiig the plasmid shipped via overight delivery Sequece data ad trace data posted to the customer's secure web site GENEMAKER APPLICATIONS Proteis Protei Expressio Codo optimized DNA for improved protei expressio Recombiat atibodies Fuctioal peptides Protei mutageesis Mutatios to study protei foldig Protei Purificatio Service Immuology Recombiat atibodies Humaizig atibodies Optioal codo optimizatio Submit sequece via secure website Gee sythesis Cloig Sequece verificatio Fial DNA product T A G A C G A T T WWW T A G A C G A T T Gee variats & variat libraries Poit mutatios, SNPs Comprehesive codo substitutio libraries Mutat libraries Custom Vectors Reshuffled vector sites Multi-resistace vector Specialized cassette drop-i vectors Custom desiged vectors PrecisioShuttle System Geomics Fuctioal geomics Sythetic chromosomes & geomes Cloe Sets & collectios Limitless possibilities Combiatios of aturally-occurrig DNA ad sythetic costructs Completely ovel sequeces ot available i ature Complete flexibility i poit or regioal deletio/additio mutatios Egieered DNA sequeces for sythetic biology studies

16 OriGee, Your Parter i Gee Research ad Beyod KEY TECHNOLOGIES AND PRODUCTS Full-legth cdna cloes, ORF cloes i expressio-ready vectors Gee sythesis: research specific gee desig RNAi research reagets: shrna, sirna, ad mirna fuctio ad detectio SYBR Gree qpcr assays for mrna ad mirna detectio, primer paels Recombiat huma proteis TrueMAB moocloal atibodies Lumiex multiplex immuoassays Cacer tissue biorepository, TMA, RPPA, ad TissueSca qpcr arrays PO Box 520 HA9 7YN, UK Tel: (0) Fax: (0) ifo@isightbio.com Web: