High Quality Primary Antibodies with Extensive Validation

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1 High Quality Primary Atibodies with Extesive Validatio Atibodies ad Immuoassays 10,000 primary atibodies with free positive cotrols Ati-tag atibodies for the detectios of recombiat proteis TrueMAB moocloal atibodies made agaist authetic protei atiges Immuoassay kits ad services for Lumiex xmap Platform

2 High Quality Primary Atibodies from OriGee OriGee offers a broad selectio of moocloal ad polycloal atibodies to huma proteis for various immuo-detectios. l0,000 primary atibodies target critical proteis coverig various pathways. TrueMAB moocloal atibodies are superior at recogizig ative protei epitopes. Made agaist authetic protei atiges with extesive validatio data. More tha 200 ew TrueMAB s added mothly with 30% off for mothly ew-releases. Iclude a free positive cotrol whe the matched over-expressio cell lysate is available. Moey back guaratee for validated applicatios. Ati-tag atibodies for the high sesitivity ad specificity detectio of recombiat proteis, icludig ati-ddk (ati-flag )*, ati-turbogfp, ati-egfp, ati-eyfp ad more. HepG2 HeLa HT29 A549 COS7 Jurkat MDCK PC12 MCF C5 ANTI-DDK MONOCLONAL ANTIBODY OriGee has developed 4C5 ati-ddk atibody for the detectio of DYKDDDDK epitope (same epitope as FLAG )* of recombiat proteis. with M2 moocloal atibody from vedor S. Both atibodies were used at 1:2000 dilutio agaist same sample (HSPA9) dilutios. Validated for Wester blot, immuoprecipitatio, immuofluorescece ad flow cytometry Higher sesitivity ad specificity compared to other vedors ati-flag * atibodies 4C5 ati-ddk moocloal atibody (TA50011) showed higher sesitivity i the side-by-side compariso Sample (HSPA9) dilutio Sample (HSPA9) dilutio 1X 1/2 1/4 1/8 1/16 1/32 1X 1/2 1/4 1/8 1/16 1/32 Agaist 4C5 ati-ddk mab Agaist vedor S M2 mab * FLAG is a trademark of Sigma-Aldrich.

3 2H8 ANTI-tGFP MONOCLONAL ANTIBODY The 2H8 ati-tgfp (turbo gree fluorescet protei) moocloal atibody has bee developed for the detectio of tgfp tag fused to the N- or C-termius of recombiat proteis. Validated for Wester blot ad immuofluorescet staiig with high specificity ad sesitivity. Made agaist a tgfp tagged recombiat protei expressed i HEK293T cell. 5A2 ANTI-eGFP MONOCLONAL ANTIBODY The 5A2 ati-egfp (ehaced gree fluorescet protei) moocloal atibody has bee developed for the detectio of egfp tag fused to the N- or C-termius of recombiat proteis. Validated for Wester blot with high specificity ad sesitivity. Made agaist a egfp tagged recombiat protei expressed i HEK293T cell F11 & 14C4 ANTI-tYFP MONOCLONAL ANTIBODY The 10F11 ad 14C4 ati-tyfp (turbo-yellow fluorescet protei) moocloal atibody has bee developed for the detectio of tyfp tag fused to the N- or C-termius of recombiat proteis. Validated for Wester blot, immuofluorescet staiig ad flow cytometry with high specificity ad sesitivity. Made agaist a full legth tyfp protei expressed i HEK293T cell. Evets TYFP

4 TrueMAB Moocloal Atibodies Made agaist authetic protei atiges TrueMAB atibodies are great tools for immuoassays that are sesitive to proteis coformatios, such as immuofluorescece, immuoprecipitatio, flow cytometry, ELISA, immuohistochemistry, high cotet screeig (HCS), atibody arrays ad more. Superior moocloal atibodies made to recogize ative protei epitopes Made agaist authetic protei atiges Extesive validatio Wester blot o cell lysates (9 cell lies with a positive cotrol) Immuohistochemistry o 24 huma ormal ad tumor FFPE tissues Immuofluorescet staiig Flow cytometry Immuo-precipitatio Icludes a free positive cotrol Multiple TrueMAB moocloal atibodies available for a sigle protei target for cross-referece validatios More tha 200 ew TrueMAB added mothly Nomiate your favorite huma protei atige(s) for TrueMAB moocloal atibody collectio at or by sedig s to atibody@origee.com. Immuohistochemical staiig of paraffi-embedded lug tissue usig ati-ercc1 mouse moocloal atibody. (TA500622, Dilutio 1:50) Ati-SHC1 mouse moocloal atibody (TA501074) immuofluorescet staiig of COS7 cells trasietly trasfected by pcmv6-entry SHC1 (RC204362).

5 TrueMAB Moocloal Atibodies For the recogitio of the ative protei epitopes TrueMAB moocloal atibodies were geerated usig recombiat huma proteis as atiges that were affiity purified uder ative coditio to preserve the proteis' coformatios. Commoly available commercial atibodies were geerated usig short peptides. The peptide atiges are iadequate to mimic the protei coformatios due to the lack of three-dimesioal structures. I particular, peptide-derived atibodies caot recogize the coformatioal epitopes which are primarily preseted o the surface of ative proteis. I compariso to peptide-derived atibodies, TrueMAB moocloal atibodies provide high sesitivity ad specificity for the recogitio of ative protei epitope's coformatioal structures. Native Epitope (IF, ELISA, FC, IP, HCS) NATIVE EPITOPES Two types of epitopes are preseted o the surface of ative protei coformatios. A. Coformatioal epitope (discotiuous epitope); B. Liear epitope. Coformatioal epitopes are abudat o the surfaces of ative proteis. The peptide atiges are iadequate to mimic the protei coformatios due to the lack of threedimesioal structures. Deatatured Proteis (Wester Blot) Applicatos Proteis Coformatios i the Samples Epitope Recogitio Wester Blot Deatured Proteis Ufolded Liear Epitopes Immuofluorescet Staiig Native Folded Proteis Native Epitopes Flow Cytometry Native Folded Proteis Native Epitopes Immuo-Precipitatio Native Folded Proteis Native Epitopes ELISA Native Folded Proteis Native Epitopes High Cotet Screeig Native Folded Proteis Native Epitopes Atibody Array Native Folded Proteis Native Epitopes Lumiex Multiplexig Native Folded Proteis Native Epitopes Immuohistochemistry Native/Partially Ufolded Proteis Coformatioal & (fixed & cross liked) Liear Epitopes

6 Moocloal Atibody Developmet (TrueMAB joit-developmet program for huma proteome) OriGee provides joit-developmet programs to geerate mouse moocloal atibodies usig huma proteis as atiges. Upo the acceptace of the request after OriGee s evaluatio, the participat cotributes a portio of the developmet cost, ad OriGee will be resposible for: Atige preparatio (purified huma proteis expressed i HEK293T cells) Mouse moocloal atibody developmet Atibody validatios (WB o a pael of cell lies, IHC o 24 tissues, & IF) Atibody productio & affiity purificatio Atige Preparatio Atibody Developmet Atibody Validatio Atibody Productio BENEFITS OF THE PROGRAMS Proteis with ative coformatio will be used as atiges. The protei-derived atibodies recogize ative epitopes with high reactivity ad specificity compared to peptide-derived atibodies Atiges ot required, OriGee will prepare the atiges OriGee provides extesive characterizatio data for atibody validatio OriGee delivers affiity purified atibodies ready for your assays Program cost: iquire at atibody@origee.com

7 Multiplexed Immuoassays for the Lumiex xmap Platform OriGee is committed to developig high quality immuoassay reagets for protei quatitatio to support biomarker discovery, validatio ad cofirmatio. TRUEPLEX HUMAN GROWTH FACTOR (11-PLEX) ASSAY KIT (AM100096) The TruePLEX huma growth factor 11-plex kit is desiged for the measuremet of 11 differet growth factors i cell culture superatat, huma sera, or plasma i cojuctio with Lumiex xmap techology. Media Flourescet Itesity 30,000 25,000 20,000 15,000 10,000 5, ,000 Huma Growth Factor Pael (11-plex) Stadard Curves PDGF-AB PDGF-BB FGF4 VEGFD FGF2 EGF HGF FLT3LG ANGPT2 PGF VEGFA ,000 10, ,000 Stadard Cocetratio (pg/ml) IMMUNOASSAY DEVELOPMENT SERVICES OriGee provides custom multiplexed immuoassay developmet for Lumiex xmap techology. OriGee s research ad developmet team possesses extesive developmet expertise utilizig varied platforms. OriGee s Uique Resources for Immuoassay Developmet Iclude: Capacities to geerate ew moocloal atibodies agaist protei atiges for the recogitio of protei s ative epitopes. FEATURES OF ORIGENE S MULTIPLEXED IMMUNOASSAYS Reduced reaget ad labor costs Smaller sample requiremets per data poit Fast reactio kietics provided by the bead array format Program cost: iquire at assays@origee.com A growig ivetory of >6,000 authetic full legth huma proteis as assay stadards

8 OriGee, Your Parter i Gee Research ad Beyod KEY TECHNOLOGIES AND PRODUCTS Full-legth cdna cloes, ORF cloes i expressio-ready vectors Gee sythesis: ay gee, ay variat, ay vector RNAi research reagets: shrna, sirna, ad mirna fuctio ad detectio SYBR Gree qpcr assays for mrna ad mirna detectio, primer paels Recombiat huma proteis ad over-expressio lysates TrueMAB moocloal atibodies Lumiex multiplex immuoassays Cacer tissue biorepository, TMA, RPPA, ad TissueSca qpcr arrays UltraMAB validated IHC atibodies OriGee USA 9620 Medical Ceter Drive, Suite 200 Rockville, MD OriGee Gee Sythesis Blue Hero Biotech th Aveue SE #100 Bothell, WA USA OriGee CHINA HuiLogSe Iteratioal Sciece & Techology Idustry Park Build 11, No.99, Kechuag Fourteeth Street, D4 Beijig, CHINA