Warranty...i. Introduction... 1
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1 USER MANUAL
2 Contents Warranty... Introducton... 1 Gettng Started... 1 Intal Inspecton... 1 Battery Installaton... 1 Setup... 2 Keypad... 9 Man Dsplay Menu Layout Alpha/Numerc Entry System Menu Date/Tme GLP Language Item # Rev D Drawng # A March YSI Incorporated. The YSI logo and Confdence Soluton are regstered trademarks of YSI Incorporated. Teflon s a regstered trademark of E. I. du Pont de Nemours and Company. Mcrosoft and Wndows are regstered trademarks of the Mcrosoft Corporaton n the Unted States and other countres. Pentum s a regstered trademark of Intel Corporaton. Radx Pont Loggng Auto Shutoff Backlght SW Verson (Software Verson) Seral # Unt ID Parameters: Setup, Dsplay, Auto Stable, and Calbraton Temperature... 20
3 Dssolved Oxygen (DO) Barometer Conductvty ph ORP Ammonum, Ntrate, Chlorde Takng Measurements Dssolved Oxygen Conductvty ph/orp Ammonum, Ntrate, and Chlorde Loggng Data Fles and Ste Lsts Fle Memory Vewng Saved Data Ste Lst Folder Delete Data Sensor Mantenance Sensor Storage Troubleshootng Help Error Messages Dssolved Oxygen Restore Default Calbraton Values Accessores / Part Numbers Declaraton of Conformty Recyclng Contact Informaton Orderng and Techncal Support Servce Informaton Data Manager Desktop Software Usng the Communcatons Saddle Manage Logged Data Real Tme Studes Confgure Instruments Care, Mantenance, and Storage Updatng Instrument Frmware General Mantenance... 58
4 Wa r r a n t y Ths page left ntentonally blank The YSI Professonal Plus Instrument (Pro Plus) s warranted for three (3) years from date of purchase by the end user aganst defects n materals and workmanshp, exclusve of batteres and any damage caused by defectve batteres. Pro Plus feld cables are warranted for two (2) years from date of purchase by the end user aganst defects n materal and workmanshp (6 months for non-feld rugged cables*). Pro Plus sensors (ph, ORP, ph/orp combo, Polarographc DO) are warranted for one (1) year from date of purchase by the end user aganst defects n materal and workmanshp (6 months for ammonum**, ntrate**, chlorde**, and Galvanc DO). Pro Plus systems (nstrument, cables & sensors) are warranted for 90 days from date of purchase by the end user aganst defects n materal and workmanshp when purchased by rental agences for rental purposes. Wthn the warranty perod, YSI wll repar or replace, at ts sole dscreton, free of charge, any product that YSI determnes to be covered by ths warranty. To exercse ths warranty, call your local YSI representatve, or contact YSI Customer Servce n Yellow Sprngs, Oho at , or vst www. YSI.com (Support tab) for a Product Return Form. Send the product and proof of purchase, transportaton prepad, to the Authorzed Servce Center selected by YSI. Repar or replacement wll be made and the product returned, transportaton prepad. Repared or replaced products are warranted for the balance of the orgnal warranty perod, or at least 90 days from date of repar or replacement. LIMITATION OF WARRANTY Ths Warranty does not apply to any YSI product damage or falure caused by: 1. falure to nstall, operate or use the product n accordance wth YSI s wrtten nstructons; 2. abuse or msuse of the product; 3. falure to mantan the product n accordance wth YSI s wrtten nstructons or standard ndustry procedure; 4. any mproper repars to the product; 5. use by you of defectve or mproper components or parts n servcng or reparng the product; 6. modfcaton of the product n any way not expressly authorzed by YSI. THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY. IN NO EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY.
5 * The warranty perod for the non-feld rugged cables (605107, , , , , ) s lsted as 6 months. However, the true workng lfe of these sensors may be 3-9 months dependng on storage and usage n solutons other than clean aqueous samples. ** The warranty for the ammonum, ntrate, and chlorde sensors (605104, , ) s lsted as 6 months. However, the true workng lfe of these sensors may be 3-9 months dependng on storage and usage n solutons other than clean aqueous samples. Introducton Thank you for purchasng the YSI Professonal Plus (Pro Plus). The Pro Plus features a waterproof (IP-67) case, backlt dsplay and keypad, user-selectable cable optons, USB connectvty, large memory wth extensve ste lst capabltes, and a rugged, rubber over-molded case. Readng the entre manual before use s recommended for an overall understandng of the nstrument s features. Gettng Started Intal Inspecton Carefully unpack the nstrument and accessores and nspect for damage. Compare receved parts wth tems on the packng lst. If any parts or materals are damaged, contact YSI Customer Servce at ( ) or the authorzed YSI dstrbutor from whom the nstrument was purchased. Battery Installaton The Pro Plus requres (2) alkalne C-cell batteres whch are ncluded wth the purchase of a new nstrument. Battery lfe depends on parameters and usage. Under normal condtons, battery lfe s approxmately 80 hours for contnuous use at room temperature. To nstall or replace the batteres: Turn the nstrument over to vew the battery cover on the back. Unscrew the four captve battery cover screws. Remove the battery cover and nstall the new batteres, ensurng correct polarty algnment on the nstrument or the removed cover. (Fgure 1) Replace the battery cover on the back of the nstrument and tghten the four screws. Do NOT over-tghten. Fgure 1. Pro Plus wth battery cover removed. Notce battery symbols ndcatng polartes. 1
6 Batteres must be nstalled n the nstrument even f powerng the unt va the USB connecton. Ths wll retan the correct date and tme f the PC s turned off. If the USB power s dsconnected and there are no batteres n the nstrument, the date and tme wll need to be reset upon subsequent power on. NOTE - On subsequent battery changes you wll have approxmately 2 mnutes to change the batteres before the clock resets. If the clock resets, the nstrument wll automatcally brng up the Date/Tme menu the next tme t s powered on n order to update ths nformaton. Ths s mportant, especally f you ntend to log data! Setup The Pro Plus nstrument has several compatble feld-rugged cable/sensor optons, each wth temperature: Cable: Cable number x Cable number x Cable number x Cable number x Cable number x Cable number x Cable number x Cable number x Avalable Sensors: DO/temp ( for lab BOD) Conductvty/temp ISE*/temp ISE*/ISE*/temp ISE*/DO/temp ISE*/conductvty/temp DO/conductvty/temp DO/conductvty/ISE*/ISE*/temp (Quatro**) *ISE (Ion Selectve Electrode) notates a port that can accept ph, ORP, Ammonum, Ntrate, Chlorde, and, n some cases, a ph/orp combnaton sensor. **Cable wll be referred to as a Quatro cable throughout ths manual. All cables come n standard lengths of 1, 4, 10, 20, and 30-meters (3.28, 13, 32.8, 65.6, and 98.4-feet) wth optons for specal order lengths up to 100-meters (328-feet) on the x cables. Contact YSI or your local representatve for addtonal nformaton. recommended for lab use or controlled condtons where a more rugged, feld cable s not necessary. These cables nclude: Cable number Cable number Cable number Cable number Cable number Cable number meter cable; sngle-juncton ph sensor 4-meter cable; sngle-juncton ph sensor 1-meter cable; sngle-juncton ORP sensor 4-meter cable; sngle-juncton ORP sensor 1-meter cable; sngle-juncton ph/orp sensors 4-meter cable; sngle-juncton ph/orp sensors Standard Pro Seres SENSOR Installaton Throughout the manual, the term sensor refers to the removable porton or electrode sensng porton of the cable assembly. For example, the DO sensor or ph sensor s the part that can be removed from a feld cable and replaced wth a new sensor. The conductvty sensor s not removable from a non-quatro cable but stll refers to the sensng porton and wll be referred to as a sensor. Ths secton covers most of the sensor nstallatons on a Professonal Seres cable bulkhead ncludng the followng sensors: Polarographc DO (black) Galvanc DO (gray) ph ph/orp Chlorde ORP Ammonum Ntrate See the next secton of ths manual for nstallaton nstructons for the Quatro cable s Conductvty/Temperature sensor. Dual sensor bulkhead ports are numbered 1 and 2, see fgure to the left. Please refer to the followng tables to determne correct sensor nstallaton nto each port of a two port cable. In addton there are several cable optons wth bult n sensors for the measurement of ph and ORP that are not consdered feld-rugged (nonreplaceable sensors, less rugged sngle-juncton sensors). These cables are 2 3
7 1010 dual cable Port 1 Optons ph ORP ph or ph/orp* ammonum chlorde ntrate Port 2 Optons ph ORP ph or ph/orp* ammonum chlorde ntrate none (port plug) * If usng a cable, a sensor must be nstalled n port 1 for correct operaton. If you nstall a ph/orp combo sensor nto a cable, ORP wll not be measured. It s not recommended to use a ph/orp combo sensor on a cable. Port 1 Optons Port 2 Optons ph Polarographc DO ORP Galvanc DO ph or ph/orp none (port plug) 1020 dual cable ammonum chlorde ntrate none (port plug) If usng a 1020 cable, nstall a ph, ORP, ph/orp, Ammonum, Ntrate or Chlorde sensor n port 1 and a DO sensor n port 2. Quatro Cable (pn: ) Port 1 Optons Port 2 Optons DO Port Optons ph ph Polarographc DO ORP ORP Galvanc DO ph or ph/ ORP* ammonum chlorde ntrate ph or ph/ ORP* ammonum chlorde ntrate none (port plug) none (port plug) CT Port Optons 5560 Conductvty/ Temperature sensor only * If usng a Quatro cable, a sensor must be nstalled n port 1 for correct operaton of port 2. If you nstall a ph/orp combo sensor nto a Quatro cable, ORP wll not be measured. It s not recommended to use a ph/orp combo sensor on a Quatro cable. Before nstallng ether dssolved oxygen sensor, the nstrument must be confgured for the sensor beng nstalled. See the Setup - Dssolved Oxygen secton of ths manual for nstrument confguraton nstructons. Falure to do ths may result n damage not covered under warranty. If usng a ph/orp combnaton probe on a or cable you can report both ph and ORP. However, t s recommended to set ISE1 as ph and ISE2 as ORP n the Sensor Setup menu. The Quatro cable bulkheads are labeled 1, 2, DO, and CT, see fgure to the left. All sensors except the Conductvty/Temperature sensor can be nstalled followng the Standard Pro Seres Sensor Installaton nstructons. Conductvty/Temperature sensor nstallaton s descrbed n the next secton. For ease of nstallaton, YSI recommends that you nstall a sensor nto port 1 frst; followed by DO nstallaton, then port 2, and lastly C/T. Frst, ensure both the sensor connector and sensor port on the cable are clean and dry. To connect the sensor, grasp the sensor wth one hand and the sensor connecton end of the cable (bulkhead) n the other. Push the sensor nto the connector on the cable untl t s properly seated and only one o-rng s vsble. Falure to properly seat the probe may result n damage. Twst the sensor clockwse to engage threads and fnger tghten (Fgure 2). Do not use a tool. Ths connecton s waterproof. Please refer to the sensor nstallaton sheet that s ncluded wth each sensor for detaled nstructons. 4 5
8 Sensor Guard Fgure 2. The mage on the left shows a clean, dry sensor beng algned wth the bulkhead. On the rght, the sensor has been pushed nto the bulkhead and s beng screwed nto place. Fgure 3. The sensor(s) wll nstall drectly n the cable bulkhead. Once nstalled, the sensor guard wll protect the sensor durng samplng (DO cap membrane not shown). Conductvty/Temperature sensor Installaton n a Quatro Cable As mentoned, the nstallaton of the Conductvty/Temperature sensor (model 5560) n a Quatro cable s dfferent from all other Pro Seres sensor nstallatons. Follow these nstructons when nstallng a conductvty/temperature sensor n a Quatro cable: Locate the C/T port and, f replacng, remove the old sensor usng the nstallaton tool to loosen the stanless steel retanng nut. Once the stanless steel retanng nut has been completely unscrewed from the bulkhead, remove the old sensor from the bulkhead by pullng the sensor straght out of the bulkhead. Apply a thn coat of o-rng lubrcant (suppled wth the sensor) to the o-rngs on the connector sde of the new sensor. Sensor Cable Bulkhead Vsually nspect the port for mosture. If mosture s found, t must be completely dred pror to sensor nstallaton Algn the connectors of the new sensor and the port. Wth connectors algned, push the sensor n towards the bulkhead untl you feel the sensor seat n ts port. You wll experence some resstance as you push the sensor nward, ths s normal Once you feel the sensor seat nto the port, gently rotate the stanless steel sensor nut clockwse wth your fngers, Do not use the tool. The nut must be screwed n by hand. If the nut s dffcult to turn, STOP, as ths may ndcate cross threadng. If you feel resstance or cross threadng at any pont, unscrew the nut and try agan untl you are able to screw the nut down completely wthout feelng any resstance. Damage to your cable/ sensor may occur f you force the parts together. Once completely nstalled, the nut wll seat flat aganst the bulkhead. At ths pont, use the tool that was ncluded wth the sensor to turn the nut an addtonal ¼ to ½ turn so t cannot come loose (fgure 4). DO NOT over tghten. Do not cross thread the sensor nut. Seat nut on face of bulkhead. Do not over tghten. Please refer to the sensor nstallaton sheet that s ncluded wth the conductvty/ temperature sensor for detaled nstructons. Installng Port Plugs n Unused Ports Fgure 4. Installaton tool used to tghten stanless steel retanng nut of 5560 conductvty/temperature sensor. As necessary, nstall a port plug nto any port that does not have an nstalled sensor. Ths wll protect the bulkhead from water damage. Port plugs and a tube of o-rng lubrcant are ncluded wth all Quatro cables. These tems can be ordered separately f needed. To nstall a port plug, apply a thn coat of o-rng lubrcant to the two o-rngs on the port plug. After applcaton, there should be a thn coat of o-rng lubrcant on the o-rngs. Remove any excess o-rng lubrcant from the o-rng and/or port plug wth a lens cleanng tssue. Next, nsert the plug nto an empty port on the bulkhead and press frmly untl seated. Then, turn the plug clockwse to engage the threads and fnger-tghten untl the plug s nstalled completely. Do not use a tool to tghten the plug. 6 7
9 Connectng the Cable to an Instrument To connect a cable, algn the keys on the cable connector to the slots on the nstrument connector. Push together frmly, then twst the outer rng untl t locks nto place (fgure 5). Ths connecton s water-proof. Keypad Fgure 5. Note the keyed connector. The cable and nstrument connectors can only be mated once the keys are properly algned. Sensor Storage When a sensor s not nstalled, the sensor and cable sensor connectors are NOT water-proof. Do not submerge the cable wthout a sensor or port plug nstalled n all avalable ports. When the cable s dsconnected, the cable s nstrument connector and the connector on the nstrument mantan an IP-67 ratng. The cable assembly s suppled wth a storage contaner, or sleeve, that nstalls on to the cable. The contaner s used for short-term storage (less than 30 days). Be sure to keep a small amount of mosture (tap water) n the contaner durng storage. Ths s done to mantan a 100% saturated ar envronment whch s deal for short-term sensor storage (see Care, Mantenance, and Storage for more detaled nformaton). Do not submerge the sensors n an aqueous soluton. The ntent s to create a humd ar storage envronment. Number Key Descrpton 1 System Opens System Menu from any screen. Use to adjust system settngs. 2 Sensor Opens Sensor Menu from any screen. Use to enable sensors and dsplay unts. 3 Calbrate Opens Calbrate Menu from any screen. Use to calbrate all parameters except temperature. 4 Fle Opens Fle Menu from any screen. Use to vew data and GLP fles, set up ste and folder lsts, and delete data. 5 Backlght Press to turn the nstrument backlght on and off and to adjust the dsplay contrast when pressed wth the left or rght arrow key. 8 9
10 Number Key Descrpton 6 Rght Arrow Use to navgate rght n alpha/numerc entry screens. Can be pressed smultaneously wth Backlght key to ncrease dsplay contrast. 7 Down Arrow Use to navgate through menus and to navgate down n alpha/numerc entry screens. 8 Power Press to turn the nstrument on. Press and hold for 3 seconds to turn off. 9 Help Press to receve hnts & tps durng operaton. 10 Enter Press to confrm selectons, ncludng alpha/numerc key selectons. 11 Left Arrow Use to navgate left n alpha/numerc entry screens. Press to return to prevous menu n all screens except alpha/numerc entry. Can be pressed smultaneously wth Backlght key to decrease dsplay contrast. 12 Ext/Escape Exts back to Run Screen. When n alpha/numerc entry screen, escapes to prevous menu. 13 Up Arrow Use to navgate through menus and to navgate up n alpha/numerc entry screens. Man Dsplay Press the Power key to turn the nstrument on. The nstrument wll brefly dsplay the splash screen wth the YSI logo then go drectly to the man run screen. The frst tme the nstrument s powered up or f the nstrument has had a battery change (wth batteres removed for more than 2 mnutes), you wll need to set the date and tme. Follow the nstructons under System Menu Date/Tme. The dsplay at the left shows the run mode (man dsplay) wth temperature n C, barometer n mmhg, DO n % and mg/l, and ph as the reported parameters. The date, tme and battery level are ndcated at the bottom of the screen. The loggng preference of Log One Sample at a tme s ndcated at the top of the screen. Ths screen also shows the message lne towards the bottom of the dsplay above the date and tme. In ths case, t doesn t show a message but messages wll appear frequently to ndcate calbraton steps, set date and tme, etc. A USB symbol wll show up on the bottom of the dsplay when connected to a PC through USB wth the communcatons saddle. The nstrument wll dsplay full battery power when t s recevng power through the USB connecton. Menu Layout Contrast the contrast adjustment can be accomplshed by pressng the backlght key and the left or rght arrow key at the same tme. Press Esc at anytme n the menus to escape back to the Run screen. The left arrow can be used to go back to the prevous menu n all screens except alpha/numerc entry screens. You must use Esc to get out of the alpha/numerc screens f you want to ext before fnshng or wthout savng changes. Functons that are enabled appear as a crcle wth a dot or a box wth a check mark Q. Dsabled functons appear as a crcle only or an empty P
11 Alpha/Numerc Entry System Menu Press System menu tems. to access any of the followng The System menu wll allow you to access the setup optons of the nstrument ncludng; Date/ Tme, GLP, Language, Radx Pont, Loggng, Auto-Shutoff, Backlght, SW (Software) Verson, Seral #, and Unt ID. Any tem wth [brackets] shows the current settng nsde the brackets. For nstance, n the example at the left, Radx Pont s currently set to [Decmal]. The brackets wll also gve a quck vsual clue as to what tems can be changed. The numerc screens wll dsplay numbers only (shown on the left). Alpha/ numerc screens wll dsplay numbers across the top and letters along the bottom rows (shown on the rght). Letters appear as a common keyboard arrangement. When an alpha or numerc character s requred, the screen wll show the alpha/ numerc entry screen. To select a character, hghlght t by usng the arrow keys to move the hghlght box over the desred selecton. Then, press Enter on the keypad to confrm the selecton. After confrmng the selecton, t wll appear n the lne at the top of the dsplay. For captal letters or lower case entry, hghlght SHIFT and press Enter on the keypad to change the characters from upper to lower case. Date/Tme Hghlght Date/Tme from the System menu. Press enter to select. Date Format Hghlght and press enter to open a sub menu for selectng the preferred date format: YY/MM/DD, MM/DD/YY, DD/MM/YY, or YY/DD/MM. Date Hghlght and press enter to use the numerc entry screen to set the correct date. To delete the entre lne of the current entry, hghlght ) and press Enter on the keypad. The! symbol functons as a backspace key n the alpha/numerc entry screens by deletng one character at a tme. Use the SPACE functon to add a space between characters. When you have fnshed enterng the correct nformaton (16 character max), hghlght <<<ENTER>>> at the bottom of the screen and press Enter on the keypad to confrm. The key cannot be used to escape to the prevous menu from an alpha/numerc entry screen. Instead, use the key to go back to the prevous menu when n alpha/numerc entry screens Tme Format Hghlght and press enter to open a submenu to select the preferred tme format from 12-hour or 24-hour. Tme Hghlght and press enter to use the numerc entry screen to set the correct tme. The date and tme wll need to be reset f a battery change takes longer than 2 mnutes. When ths occurs, the Date/Tme menu wll automatcally appear upon power up and requre you to set the date and tme.
12 GLP The GLP or Good Laboratory Practce fle saves detaled nformaton about calbratons. It also ncludes dagnostc nformaton about the sensors. Calbratons are logged nto a fle, the GLP, for later revew as needed. A sngle GLP fle s utlzed to store all calbraton records and s capable of storng 500 records. Once the GLP fle s full, the nstrument wll begn to overwrte the oldest record wth each new calbraton record. In order to keep all of your GLP records, perodcally download the GLP to Data Manager and export t to another program. Otherwse, the unt wll overwrte the oldest record once the memory s full. Also, snce Data Manager saves GLP fles under the Unt ID, you must perodcally export and rename the GLP fle on your PC or t wll be overwrtten each tme you upload the GLP fle from the nstrument. Several calbraton parameters are saved for each calbraton record ncludng optonal ones that can be enabled by the user. Standard parameters nclude date/tme stamp, calbraton method, and sensor nformaton. Optonal, user selectable parameters nclude User ID, Probe ID, and User Felds 1 and 2. The sensor specfc nformaton that s saved wth each calbraton pont s dfferent for each sensor. The sensor specfc values saved are: Conductvty Method (Spec Cond, Cond, Salnty) Cal Value (value of calbraton soluton) Sensor Value (Cell Constant) Temperature Reference (User selected n Sensor Setup menu) Temperature Compensaton Coeffcent %/ C (User selected n Sensor Setup menu) TDS Constant (User selected n Sensor Setup menu) Temperature Cal Cell Constant Calbrate Status DO Method (%, mg/l) Cal Value Sensor Value (Sensor Current) Sensor Type (Polarographc/Galvanc) Membrane Type (Teflon Black, PE Yellow, PE Blue) Salnty Mode (user entered value f n Manual Salnty Mode) Temperature Barometer Calbrate Status ph (up to 6 calbraton ponts) Buffer Value Sensor Value (mv) Temperature Slope (mv/ph) Slope (% of deal) Calbrate Status ORP Cal Soluton Value Sensor Value Temperature Calbrate Status Ammonum Buffer Value Sensor Value (mv) Temperature Calbrate Status Chlorde Buffer Value Sensor Value (mv) Temperature Calbrate Status Ntrate Buffer Value Sensor Value (mv) Temperature Calbrate Status 14 15
13 An example of a GLP record (Operaton performed s sngle pont % DO Calbraton) *** Calbrate DO% *** Date 02/03/09 MM/DD/YY Tme 12:14:57PM 12-hour User ID: tech 1 Probe ID 08D Method DO Ar Calbrate Cal Value: % Sensor Value: uA Sensor Type Polarographc Membrane Type 1.25 PE Yellow Salnty Mode Auto Temperature 23.9 C Barometer mmhg Calbrate Status Calbrated GLP Settngs In the System menu, hghlght GLP and press enter to vew and modfy the GLP settngs. Hghlght Optons and press enter to access User ID, Probe ID, User Defned Felds, and Re-Cal Prompt. User ID may be used to dentfy the person calbratng the nstrument. Hghlght User ID and press enter to select, edt, or delete a User ID from a lst of prevously entered IDs. Or, hghlght Add New and press enter to create a new User ID usng the alpha/numerc entry screen. The User ID may also be changed n the Calbraton menu durng the calbraton process. The selected User ID wll be stored n the GLP fle wth each calbraton record. A User ID could be a person s ntals or badge number. The character lmt s 16 characters. Probe ID s stored wth the calbraton record and may be used to dstngush one cable/probe assembly from another, typcally by seral number. Hghlght Include Probe ID and press enter to turn ths functon on (Q) and off (P). Hghlght Probe ID and press enter to add, vew, edt, delete, or select a Probe ID. The Probe ID may also be changed n the Calbraton menu durng the calbraton process. The character lmt s 16 characters. User Felds 1 and 2 are stored wth the calbraton record and may be used to enter other parameters pertnent to the user, such as weather condtons, elevaton, etc. Hghlght Include User Feld 1 or Include User Feld 2 and press enter to turn ths functon on and off. Hghlght User Feld 1 or User Feld 2 and press enter to add, delete, vew, edt, or select a User Feld. The character lmt s 16 characters. When enabled, a prompt for selectng a User Defned Feld wll appear durng the calbraton process. Re-Cal Prompt may be used to remnd the user to perform a calbraton. To set a tme nterval, hghlght the parameter you wsh to be remnded about and press enter to access the numerc entry screen. Enter a value n days and press enter to confrm the remnder tme. To turn off the Re-cal prompt, set the remnder to zero (0) days (ths s the default). The Securty secton of the GLP menu s a password protected area. Ths area ncludes optons to set a new password and to lock access to the calbraton menu. When frst vewng the securty menu, you wll be requred to enter a password. Use the shft on the alpha/numerc screen to swtch to lower case f necessary and enter ys123. Ths s the default password. Protect Cal can be enabled (Q) or dsabled (P). When enabled, the user must know and enter the nstrument s password to enter the calbraton menu opton. Hghlght Protect Cal and press enter to enable or dsable ths feature. Set Password allows a user to set the securty password. Hghlght Set Password, press enter, and use the alpha/numerc entry screen to set the new password. The password can have up to 16 characters. Contact YSI Techncal Support at envronmental@ys.com or f you forget or msplace your password. Once a password s set, and the GLP securty screen exted, a password must be entered to make changes under GLP securty. Keep passwords n a safe place
14 Language Radx Pont Loggng The Pro Plus can be confgured to dsplay all text n Englsh, Spansh, French, German, Portuguese, Italan, Norwegan, Smplfed Chnese, Tradtonal Chnese, or Japanese. From the factory, the nstrument ncludes Englsh, Spansh, and French language optons. The other language optons can be downloaded from Once the approprate language fle s n the nstrument, press System, hghlght Language, and press enter. Hghlght the desred language and press enter to confrm. Radx Pont allows the user the opton to choose between a comma or a decmal n numerc dsplays. For example, 1.00 becomes 1,00 when Use Comma s selected. Hghlght Use Decmal or Use Comma and press enter to make your selecton. From the System menu, hghlght Loggng and press enter to vew or change the loggng optons. Loggng optons nclude Use Ste Lst, Use Folder Lst, Contnuous Mode, and Interval. Check the box for Contnuous Mode f you want to log samples contnuously at a specfc tme nterval. To set the length of tme between logged samples, hghlght Interval and press enter. Enter the nterval as HH:MM:SS. Ths nterval wll dsplay at the top of the screen when you select the Start Loggng opton n run mode. To log one sample at a tme, uncheck Contnuous Mode. When Contnuous Mode s unchecked, Log One Sample wll appear at the top of the run screen. Auto Shutoff Auto Shutoff powers the nstrument off after a user specfed tme perod. Hghlght Auto Shutoff and press enter. Usng the alpha/numerc entry screen, enter a value between 0 and 360 mnutes. To dsable auto shutoff, set the value to 0 (zero). Backlght SW Verson (Software Verson) Backlght can be set to Automatc or Manual. Automatc turns the backlght on when you turn the nstrument on and when you press any key. Manual allows you to turn the backlght on or off wth the backlght key. When n Automatc mode, the nstrument wll turn the backlght off 60 seconds after the last key press. The nstrument wll reset the 60 second tme perod every tme a key s pressed. The lghted keypad wll turn off after approxmately 20 seconds. SW Verson shows the nstrument s software verson. The nstrument s software can be updated va There you wll fnd the new software fles and nstructons on how to update the nstrument. There s no need to send the nstrument back to the factory for upgrades. Use Ste Lst and Use Folder Lst are optonal ways of flng or taggng your logged data ponts. If these settngs are enabled, you wll be prompted to select a Ste and/or Folder to tag to the logged data pont. See the Fle and Ste Lsts secton of ths manual for nformaton on creatng Ste and Folder Lsts. Seral # Seral # shows the nstrument s seral number and allows you to match t wth the number engraved on the back of the nstrument s case
15 Unt ID Unt ID s used to dentfy nstruments n the Data Manager software program that was ncluded wth your nstrument. It s also used to dentfy GLP fles, Ste Lsts, Confguraton fles, and Data fles transferred from the nstrument to the PC. The default Unt ID s the nstrument s seral number. To modfy the Unt ID, hghlght Unt ID, press enter and then use the alpha/numerc entry screen. The character lmt s 16 characters. Pa r a m e t e r s : S e t u p, D s p l a y, Auto Stable, and Calbraton The followng secton s separated by parameter and wll dscuss sensor setup, dsplay optons, auto stable features, and calbraton procedures for each parameter. The sectons are separated by parameter due to the versatlty of the Pro Plus. You may focus solely on the parameters of your choce. For the hghest accuracy, calbrate or verfy each sensor regularly. For your convenence, YSI offers 5580 Confdence Soluton whch allows you to check the accuracy of ph, conductvty, and ORP readngs to help determne f a sensor calbraton s necessary. If you receve an error message durng a calbraton that ndcates questonable results, you have the opton to ether accept or declne the calbraton. YSI recommends that you declne a questonable calbraton snce acceptng t may result n erroneous data. After declnng a questonable calbraton, ensure the sensor s clean, the calbraton soluton s good, the calbraton vessel s clean, and that you are enterng the correct calbraton value f enterng manually. Then, try to recalbrate the sensor. If you contnue to have problems, see the Troubleshootng secton of ths manual. Temperature All probe/cable assembles, except the Quatro, have a bult-n temperature sensor. The Quatro cable shps wth a Conductvty/Temperature sensor that must be nstalled on the cable. Temperature calbraton s not requred nor s t avalable. temperature unts of F, C, or K and press enter to confrm the selecton. Only one temperature unt may be dsplayed at a tme. You may also choose not to dsplay temperature. If you choose not to dsplay temperature, other parameters that requre a temperature readng wll stll be temperature compensated. Dssolved Oxygen (DO) DO sensors can be used on X, X, X, and Quatro cables. Preparng the DO Sensor for the Frst Tme The dssolved oxygen sensor s shpped wth a dry, protectve red cap that wll need to be removed before usng. It s very mportant to put a new membrane wth electrolyte soluton on the sensor after removng the red cap. Prepare the membrane soluton accordng to the nstructons on the bottle. After mxng, allow the soluton to st for 1 hour. Ths wll help prevent ar bubbles from later developng under the membrane. Ensure you are usng the correct electrolyte soluton for the correct sensor. Galvanc sensors utlze electrolyte wth a lght blue label and Polarographc sensors utlze electrolyte wth a whte label. The dssolved oxygen sensor s suppled wth cap membranes specfc to the sensor type ordered (Polarographc or Galvanc). 5912, 5913, and 5914 membrane kts are for Galvanc sensors and the 5906, 5908, and 5909 membrane kts are for Polarographc sensors. See the Setup - Dssolved Oxygen secton of ths manual for more nformaton on the dfferent types of membranes avalable from YSI. Remove the red cap by pullng t straght off the sensor tp. Dscard or save for later use durng long term storage. Thoroughly rnse the sensor tp wth dstlled or deonzed water. Fll the cap membrane 3 4 full of electrolyte soluton, then tap the cap wth a fnger to release any trapped ar. Be careful not to touch the membrane porton of the cap. Thread the membrane cap onto the sensor, moderately tght. Do not use a tool. It s typcal for some of the electrolyte soluton to spll over. For detaled nstructons on changng a membrane cap, see the Care, Mantenance, and Storage secton of ths manual. To set the unts, press Sensor, hghlght Dsplay and press enter. Hghlght Temperature and press enter. Hghlght the desred 20 21
16 Setup - Dssolved Oxygen Press Sensor enter., hghlght Setup and press enter. Next, hghlght DO and press Enabled allows you to enable or dsable the Dssolved Oxygen functon. Hghlght Enabled and press enter to actvate(q) or deactvate(p) dssolved oxygen. Dsable dssolved oxygen f you do not have a dssolved oxygen sensor connected to the nstrument. If a sensor s Enabled that sn t connected to the nstrument, the dsplay wll show an unstable, false readng,?????, or next to the unts. Sensor Type sets the type of oxygen sensor beng used: ether Polarographc (black) or Galvanc (grey). Hghlght Sensor Type and press enter. Hghlght the correct sensor type nstalled on the cable and press enter to confrm. If usng a ProBOD sensor/cable assembly, the sensor type should be set to polarographc. The Pro Plus has two compatble sensors for use wth a feld cable: Polarographc Ths sensor has a black sensor body and s engraved wth the model number Galvanc Ths sensor has a grey sensor body and s engraved wth the model number In terms of physcal confguraton, membrane materal, and general performance, YSI Professonal Seres Galvanc dssolved oxygen sensors are exactly lke the Professonal Seres Polarographc sensors. The advantage of usng Galvanc sensors s convenence. Galvanc sensors provde for an nstant-on sensor wthout the need for warm-up tme but ths affects the lfe of the sensor. Polarographc sensors last longer and have a longer warranty but requre a 5-15 mnute warmup tme before use or calbraton. u IMPORTANT The nstrument default settng s Galvanc. Please change the Sensor Type to match the correct sensor. If you observe readngs very close to 0 or extremely hgh readngs (.e. 600%), your Sensor Type settng (Polarographc or Galvanc) may be set ncorrectly and you should mmedately ensure t matches the sensor nstalled on your cable. Membrane sets the type of membrane used on the dssolved oxygen sensor. Hghlght Membrane and press enter. Hghlght the correct membrane type nstalled on the sensor and press enter to confrm. The DO sensor s suppled wth membranes specfc to the sensor type ordered and are color coded as descrbed n the followng tables. Galvanc membrane kts: Item Color Materal Descrpton 5912 Black 1 ml Teflon Tradtonal membrane materal 5913 Yellow 1.25 ml polyethylene Improved response tme and less flow dependence than Teflon Shps standard wth the sensor Blue 2 ml polyethylene Less flow dependence than 1.25 ml but somewhat slower response Polarographc membrane kts: Item Color Materal Descrpton 5906 Black 1 ml Teflon Tradtonal membrane materal 5908 Yellow 1.25 ml polyethylene Improved response tme and less flow dependence than Teflon Shps standard wth the sensor Blue 2 ml polyethylene Less flow dependence than 1.25 ml but somewhat slower response Selectng a Dssolved Oxygen Membrane: Membrane Type Flow Dependence After 4 Mnutes Typcal Response Tme - 95% 5912, Black 60% 18 seconds 5913, Yellow 25% 8 seconds 5914, Blue 18% 17 seconds 22 23
17 Local DO allows for localzed DO% measurements. Ths sets the calbraton value to 100% regardless of the alttude or barometrc pressure. Hghlght Local DO and press enter to enable (Q) or dsable (P) ths functon. Local DO s a method for the Pro Plus to factor n the barometrc pressure on each DO measurement. In essence, f the barometrc pressure changes you wouldn t notce the dfference n the DO% readngs n ar-saturated water or water-saturated ar. Local DO s deal for EU complance. When Local DO s enabled, an L wll appear next to DO% on the run screen. DO mg/l readngs are unaffected by the selecton of DO Local. LDS (Last Dgt Suppresson) rounds the DO value to the nearest tenth;.e mg/l becomes 8.3 mg/l. Hghlght LDS and press enter to enable (Q) or dsable (P) ths functon. Dsplay - Dssolved Oxygen Press Sensor, hghlght Dsplay and press enter. Hghlght DO and press enter. All DO unts can be dsplayed smultaneously. Hghlght the unt(s) and press enter to actvate (Q) or deactvate (P) unts from the run screen. Note - You wll not be able to dsplay dssolved oxygen unless t s Enabled n the Sensor Setup menu frst, see prevous secton. s acheved) and press enter to confrm. The Auto Stable Senstvty can be decreased or ncreased. Hghlght Senstvty and use the left and rght arrow keys to slde the bar. The more senstve you make t (larger black bar) the harder t s to acheve stablty n a changng envronment. The Auto Stable system works by examnng the prevous 5 readngs, computng the percent change n the data and comparng that change aganst a % threshold value. The % threshold value s determned by the Senstvty bar settng. The followng chart can be used as a gude when settng the Senstvty bar. Senstvty selected by User Most Senstve, Senstvty bar s set to the far rght Example: % Data Varance Threshold 0.05% % % % 0 - Least Senstve, Senstvty bar s set to the far left 2.5% DO % wll show DO readngs n a percent scale from 0 to 500%. DO mg/l wll show DO readngs n mllgrams per lter (equvalent to ppm) on a scale from 0 to 50 mg/l. DO ppm wll show DO readngs n parts per mllon (equvalent to mg/l) on a scale from 0 to 50 ppm. Auto Stable - Dssolved Oxygen Auto Stable ndcates when a readng s stable.when Auto Stable s enabled, AS wll blnk next to the parameter untl t s stable. Once the parameter s stable, AS wll stop blnkng. To enable Auto Stable, press Sensor, hghlght Auto Stable and press enter. Hghlght DO and press enter. Hghlght Enabled and/or Audo Enabled (nstrument wll beep when the stablty The nstrument obtaned the followng data: Readng # DO% Readng # DO% Readng # DO% Readng # DO% Readng # DO% The nstrument s programmed to determne the mnmum and maxmum data value over the prevous 5 samples, and to compute the percent dfference between those values. In ths example, that gves a percent change of: % Change = 100 * (( ) / 95.3) % Change = 0.83% In ths example, f the Senstvty bar s set to the far rght, the Auto Stable requrement would not be met and AS would contnue to blnk. However, f the senstvty bar s set to the medan threshold (1.275%), the Auto Stable requrement would be met and AS would dsplay steadly on the dsplay. Wthn the Auto Stable menu, you can also choose to Hold All Readngs for as many parameters as you set for Auto Stable. For nstance, f DO and ph have 24 25
18 Auto Stable and Hold All Readngs enabled, then the dsplay wll hold the readngs once DO and ph have both reached ther Auto Stable settngs. You must press the Esc key to release the held dsplay n order to take subsequent readngs Hold All Readngs must be reactvated after each use! Calbraton - Dssolved Oxygen The Pro Plus offers several optons for calbratng dssolved oxygen: DO% n water saturated ar, DO mg/l and DO ppm n a soluton of known dssolved oxygen determned by a Wnkler Ttraton, and a Zero pont. If performng a zero pont calbraton, you must also perform a %, mg/l, or ppm calbraton followng the zero calbraton. For both ease of use and accuracy, YSI recommends performng the followng 1-pont DO % water saturated ar calbraton: Salnty Correcton The last feature n the Sensor menu s the Salnty correcton value whch s used to calculate the dssolved oxygen mg/l and ammona readngs when a conductvty sensor s not n use. Press Sensor, hghlght Salnty, and press enter. Then, use the numerc entry screen to enter the Salnty value of the water you wll be testng from 0 to 70 ppt. If usng a cable wth a conductvty sensor, the salnty measured by the conductvty sensor wll be used n the DO and ammona mg/l calculatons and As Measured wll be dsplayed next to Salnty n the Sensor menu. As the salnty of water ncreases, ts ablty to dssolve oxygen decreases. For example, fully oxygenated 20 C water at sea level wth zero salnty wll hold mg/l of dssolved oxygen. If that same sample had a salnty value of 9 ppt, then t would hold mg/l of dssolved oxygen. Therefore, to obtan accurate mg/l readngs, t s mportant to know the salnty of the water you wll be testng and to nput that value nto the nstrument. The salnty of fresh water s typcally ppt and seawater s typcally 35 ppt. You wll also have the opportunty to enter or modfy the Salnty correcton value durng DO calbraton. It s not necessary to calbrate n both % and mg/l or ppm. Calbratng n % wll smultaneously calbrate mg/l and ppm and vce versa. Calbratng DO % n Water Saturated Ar: 1-Pont Calbraton The suppled sensor storage contaner (a grey sleeve for a sngle port cable or a screw on plastc cup for the dual-port and Quatro cables) can be used for DO calbraton purposes. Mosten the sponge n the storage sleeve or plastc cup wth a small amount of clean water. The sponge should be clean snce bacteral growth may consume oxygen and nterfere wth the calbraton. If usng the cup and you no longer have the sponge, place a small amount of clean water (1/8 nch) n the plastc storage cup nstead. Make sure there are no water droplets on the DO membrane or temperature sensor. Then nstall the storage sleeve or cup over the sensors. The storage sleeve ensures ventng to the atmosphere. If usng the cup, screw t on the cable and then dsengage one or two threads to ensure atmospherc ventng. Make sure the DO and temperature sensors are not mmersed n water. Turn the nstrument on and wat approxmately 5 to 15 mnutes for the storage contaner to become completely saturated and to allow the sensors to stablze. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. User ID wll appear automatcally. Select None f you do not want a User ID stored wth the calbraton. When enabled, these IDs are stored wth each calbraton record n the GLP fle
19 After selectng your User ID and/or Probe ID f approprate, hghlght DO and press enter. Hghlght DO % and press enter to confrm. The nstrument wll use the nternal barometer durng calbraton and wll dsplay ths value n brackets at the top of the dsplay. Hghlght Barometer and press enter to adjust t f needed. If the barometer readng s ncorrect, t s recommended that you calbrate the barometer. Note - the barometer should be readng true barometrc pressure (see Barometer secton for more nformaton on true barometrc pressure). If the value s acceptable, there s no need to change t or perform a barometer calbraton. The Salnty value dsplayed near the top of the screen s ether the salnty correcton value entered n the Sensor menu or the Salnty value as measured by the conductvty sensor n use and enabled. If you are not usng a conductvty sensor, the Salnty correcton value should be the salnty of the water you wll be testng. Hghlght Salnty and press enter to modfy ths settng f necessary. See the Salnty Correcton secton of ths manual for more nformaton. Wat for the temperature and DO% values under Actual Readngs to stablze, then hghlght Accept Calbraton and press enter to calbrate. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled n the GLP menu, you wll be prompted to select these nputs and then press Cal to complete the calbraton. The message lne at the bottom of the screen wll dsplay Calbratng Channel... and then Savng Confguraton.... Calbratng DO% n Water Saturated Ar: 2-Pont Calbraton wth Zero Soluton Place the sensor n a soluton of zero DO. A zero DO soluton can be made by dssolvng approxmately 8-10 grams of sodum sulfte (Na 2 SO 3 ) nto 500 ml tap water or DI water. Mx the soluton thoroughly. It may take the soluton 60 mnutes to be oxygen-free. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght DO and press enter. Hghlght Zero and press enter. Wat for the temperature and DO% values under Actual Readngs to stablze, then press enter to Accept Calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select the felds and then press Cal to complete the calbraton. The screen wll then prompt for a follow-up second pont calbraton. Hghlght DO% and press enter to contnue wth the next calbraton pont. Rnse the sensor of any zero oxygen soluton usng clean water. Then follow the steps under Calbratng DO % n Water Saturated Ar to complete the second pont. Calbratng n mg/l or ppm as a Ttraton: 1-Pont Calbraton Place the sensor nto an adequately strred sample that has been ttrated to determne the dssolved oxygen concentraton. Allow the sensor to stablze. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght DO and press enter. Hghlght DO mg/l or ppm and press enter
20 Calbratng n mg/l or ppm as a Ttraton: 2-Pont Calbraton wth Zero Soluton Place the sensor n a soluton of zero DO. Hghlght Calbraton value and press enter to manually nput the sample s dssolved oxygen value. Hghlght Accept Calbraton and press enter once the temperature and Dssolved Oxygen readngs stablze. Or, press Esc to cancel the calbraton. If User Feld 1 or 2 are enabled n the GLP menu, you wll be prompted to select the felds after selectng Accept Calbraton. After makng your selecton, press Cal to complete the calbraton. After completng the calbraton, the message lne wll dsplay Calbratng Channel... and then Savng Confguraton.... A zero DO soluton can be made by dssolvng approxmately 8-10 grams of sodum sulfte (Na 2 SO 3 ) nto 500 ml tap water. Mx the soluton thoroughly. It may take the soluton 60 mnutes to be oxygen-free. Press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght DO and press enter. Hghlght Zero and press enter. Wat for the temperature and DO% values under Actual Readngs to stablze, then press enter to Accept Calbraton. If User Feld 1 or 2 are enabled, you wll be prompted to select the felds and then Press Cal to complete the calbraton. The screen wll then prompt for a follow-up second pont calbraton. Barometer All Professonal Plus nstruments contan an nternal barometer. Dsplay - Barometer Press Sensor, hghlght Dsplay and press enter. Hghlght Barometer and press enter. The measurement unt optons are: mmhg, nhg, mbar, PSI, kpa, or Atm. Only one unt can be dsplayed at a tme. Select None f you do not want to dsplay a barometrc pressure readng. Whether or not you choose to dsplay the barometer readng, the barometrc pressure wll stll be used for calbratng DO% and for compensatng for pressure changes f Local DO s enabled. Calbraton - Barometer The barometer n the nstrument s calbrated at the factory. If the barometer requres calbraton, press Cal. Hghlght Probe ID or User ID f you wsh to add, select, edt, or delete an ID. Probe ID must be enabled n the System GLP menu to appear n the Calbrate menu. When enabled, these IDs are stored wth each calbraton record n the GLP fle. After selectng the Probe ID and/or User ID f approprate, hghlght Barometer and press enter. Hghlght the desred unt and press enter. Hghlght the desred calbraton unts (mg/l or ppm) and press enter to contnue wth the next pont. Rnse the sensor of any zero oxygen soluton usng clean water. To complete the second calbraton pont, follow the steps under Calbratng n mg/l or ppm as a Ttraton: 1-Pont Calbraton
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