MelanoBronze Natural Tanning Activator

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1 MelanoBronze Natural Tanning Activator

2 MelanoBronze Acetyl tyrosine Substrate for tyrosinase enzyme in melanin synthesis Vitex agnus-castus extract Induction of melanin synthesis in melanocytes

3 MelanoBronze MelanoBronze is a combination of two actives leading to an accelerated melanin formation in the skin Melanin is the body s own sun protecting system preventing sun burn and photo aging in the skin MelanoBronze stimulates skin tanning even without sun exposure

4 Melanocyte Stratum corneum Stratum granulosum Keratinocyte Epidermis Stratum spinosum Cell nucleus Dendrite of melanocyte Melanocyte Melanosome Basal layer Dermis

5 Mechanism of Skin Pigmentation α MSH MC1R camp tyrosinase tyrosin DOPA DOPA quinone DOPA chrom Cysteinyl DOPA melanocyte DHICA eumelanin pheomelanin

6 Acetyl Tyrosine Stable, water-soluble substrate for tyrosinase tyrosinase acetyl tyrosine DOPA melanin The reaction of tyrosine to DOPA by tyrosinase is the rate-limiting step in melanin formation. Result: tanned skin and increased UV self-protection of the skin

7 The Role of α-msh on Tanning pituitary gland αmsh bloodstream Receptor binding induction of melanin synthesis tyrosinase tyrosine melanin

8 Processing of POMC N 267 amino acid residues proopiomelanocortin (POMC) C ACTH beta-endorphin 13 αmsh

9 Beta-Endorphin and Pigmentation The beta-endorphin/μ-opiate receptor system is expressed in melanocytes and closely associated with melanosomes beta-endorphin μ-opiate receptor Melanocyte dendricity, proliferation and pigmentation are upregulated Kauser et al Journal of Investigative Dermatology

10 Monk's Pepper Berries Contain Phyto-Endorphins Large shrub growing in mediterranean region and Asia Berries from Monk's pepper contain substances that bind to the opioid receptor (Phyto- Endorphins) Chaste tree (Vitex agnus-castus)

11 Monk s Pepper Berries Traditional Medicine Used since ancient Greek to treat injuries and inflammation In the middle ages, monks used dried berries. They probably experienced a sensation of well-beeing

12 Binding to the Opioid Receptor Inhibition of opioid receptor binding by an ethanolic extract of Vitex agnus-castus expressed as IC 50 -values (μg/ml) opioid receptor μ κ δ B. Meier et al. (2000) Phytomedicine 7(5):

13 MelanoBronze: Composition Vitex agnus-castus extract containing: Acetyl Tyrosine 6% Glycerin 20% Alcohol 20%

14 MelanoBronze Natural tanning induced by two mechanisms:

15 MelanoBronze MelanoBronze phyto-endorphin

16 MelanoBronze MelanoBronze phyto-endorphin acetyl tyrosine

17 MelanoBronze MelanoBronze phyto-endorphin acetyl tyrosine melanin

18 MelanoBronze for Pigmentation with Sun UV αmsh tyrosinase pigmentation UV+ MelanoBronze αmsh beta-endorphin activity tyrosinase + acetyl tyrosine pigmentation

19 MelanoBronze for Pigmentation without Sun MelanoBronze beta-endorphin activity tyrosinase + acetyl tyrosine pigmentation Acetyl Tyrosine no tyrosinase activity! + acetyl tyrosine no pigmentation!

20 In Vitro: Specific Stimulation of Melanin Synthesis in Melanocytes % Melanin (ug/ml) Control 0.03% 0.13% 0.06% Vitex agnus-castus in the cultur medium S-091

21 In Vivo Stimulation of Skin Tanning Study design Test products: test cream cremes with 2% and 5% MelanoBronze creme with only acetyl tyrosine (same concentration as in 5% MelanoBronze) Number of test persons: 20 of age 20 to 55 Application: twice daily for 7 days on the inner side of forearm Irradiation: every day with 1.1 MED Test parameter: skin tanning (Chromameter)

22 Result Tanning effect (decrease in chromameter units relative to baseline %) Control cream 2% MelanoBronze 5% MelanoBronze S-106 Double tanning effect of acetyl tyrosine and Vitex agnuscastus extract

23 MelanoBronze DHA MelanoBronze DHA (common tanning ingredient) Stimulation of melanin synthesis in melanocytes Browning of the skin surface by a chemical reaction of DHA with proteins (Maillard reaction) Protection against solar irradiation No protection against solar irradiation Slow but long lasting effect Fast but irregular browning

24 Tanning without Sun (after 28d) Chromameter reading d 28 d 0 [a.u.] Placebo 2% MelanoBronze 5% MelanoBronze S-129

25 MelanoBronze Protective Tanning Enhancer Claims MelanoBronze provides nice regular tanning by stimulating the natural melanin formation - with UV irradiation - without UV irradiation MelanoBronze leads to enhanced pigmentation and protects your skin against UV-induced damage.

26 Applications Daycreams MelanoBronze gives a natural tan and protects against photo-aging Sun protection products Sunscreens normally do prevent the formation of a rapid tanning MelanoBronze in sun protection products speeds up the tanning process Self tanning products MelanoBronze induces the skin's own melanin formation, enforcing your self tanning products

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