HPLC Solvents and mobile phases
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1 HPLC Solvets ad mobile phases
2 CARLO ERBA Reagets A ew reality for laboratory ad idustry CARLO ERBA Reagets, a emiet brad with a wellkow history, operates at a Iteratioal level i Aalytical ad Idustrial Chemistry for laboratory, idustry, o the Italia market, ad eve i Molecular ad Cellular Biology sectors. CARLO ERBA Reagets ows advaced maufacturig plats icludig three busiess premises i Italy, Frace ad Spai.
3 HPLC Leader o the market of solvets for chromatography ad trace aalysis, CARLO ERBA Reagets exteded its rage of solvets for HPLC i order to satisfy the ever icreasig demad requiremets i terms of equipmet ad detectio methods, particularly for impurities which, by iteractio, ca affect the result s reliability. Our solvets for HPLC meet perfectly the requiremets of this aalytical techique by guarateeig the optimal specificatios o the followig elemets: Purity No volatile residue cotet UV trasmissio CARLO ERBA Reagets Grades RS HPLC Preparative RS HPLC RS HPLC Gradiet RS HPLC Ultra Gradiet / Gold RS HPLC/MS Method of aalysis HPLC HPLC HPLC Preparative Isocratic Gradiet LC-MS I this easy-to-cosult documet, we propose to you a choice of products specially adapted to the preparatio ad aalysis of your samples i HPLC: Solvets ad bleds for LC-MS Solvets for HPLC gradiet Solvets for HPLC isocratic Solvets for preparative HPLC Mobile phases Io pair reagets
4 Solvets ad bleds for LC-MS LC-MS requires the use of solvets with a miimum impurities which could ifluece the baselie ad thus reduce the aalysis sesitivity. CARLO ERBA Reagets iovates by filterig its solvets for LC-MS at 0.1 μm to satisfy the best purity, optical trasmissio ad o particle criteria. The presece of iorgaic ios ca make the idetificatio ad quatificatio of impurities impossible by formig clusters, givig false peaks, damagig the device. They also have a impact o the service life of the HPLC colums. Water LC-MS Water is ofte used i LC-MS as a solvet or a premixed additive for mobile phases. Mior impurities preset i the water load the LC colums as soo as the aalysis starts ad ca be salted out i gradiet mode. CARLO ERBA Reagets proposes a gradiet quality LC-MS water which ca be used both for UV ad MS detectio. CARLO ERBA Reagets proposes solvets with a very high purity whose specificatios satisfy the requiremets of LC-MS. Reserpie test => tested specifically for a LC-MS applicatio Abscece of a peak greater tha that of the reserpie (609 amu) betwee 50 ad amu for a cocetratio of 50 ppb Low metal io cotet (<50 ppb) Purity better tha % High UV trasmissio Excellet baselie quality i gradiet, low backgroud oise No volatile residue less tha 2 ppm Filtratio at 0.1 μm Coditioed uder itroge ad a cotrolled atmosphere Quality suitable for use i ULC-MS Zoom of 150 to 2000 m/z of the mass spectrum of acetoitrile obtaied by TIC aalysis ( m/z) ESI(+)
5 Solvets ad bleds for LC-MS LC-MS bleds CARLO ERBA Reagets elarges its offer ad proposes ready-to-use solutios amog the most used mobile phases, which brig you: Produced from LC-MS quality solvets ad specifically tested for LC-MS couplig, these solutios guaratee you : A time savig Test i LC gradiet A precise compositio High UV trasmissio The assurace of a LC-MS quality Solvet purity > % Precise additive cotet Low metal io cotet We propose a wide rage of reagets for LC-MS: from «classic» solvets up to the dissolvig of the most used additives i your mobile phases: Product Quality Pkg Code Acetoitrile LC/MS 1 L ,5 L Ethyl acetate LC/MS 1 L Methaol LC/MS 1 L ,5 L Propaol-2 LC/MS 1 L Water LC/MS 1 L ,5 L Acetic acid LC/MS 10 x 1 ml x 2,5 ml ml Formic acid LC/MS 10 x 1 ml x 2,5 ml ml Trifluoroacetic acid LC/MS 10 x 1 ml x 2,5 ml ml Acetoitrile + 0.1% v/v formic acid LC/MS 1 L ,5 L Acetoitrile + 0.1% v/v trifluoroacetic acid LC/MS 1 L ,5 L Water + 0.1% v/v formic acid LC/MS 1 L ,5 L Methaol + 0.1% v/v formic acid LC/MS 1 L ,5 L Methaol + 0.1% v/v trifluoroacetic acid LC/MS 1 L ,5 L
6 Solvets for HPLC gradiet We propose several rages for the gradiet mode aalysis: Gold Ultragradiet, Plus Gradiet. The gradiet cotrol of elutio ad drift AT with critical wavelegths of our HPLC solvets Gold ad Plus guaratee a peak free baselie. Their optimal sesitivity allows you to evaluate i the best possible way the impurities of your samples. To make sure that o particle i the mobile phase will hider your aalyses, we carry out a microfiltratio of our GOLD solvets at 0.1 μm ad for HPLC Gradiet Plus at 0.2 μm. The GOLD quality is adapted to the aalysis by UPLC-MS. Product Quality Pkg Code Acetoitrile HPLC - Gold 1 L ,5 L L HPLC Plus Gradiet ACS-Reag.Ph.Eur.-Reag.USP 1 L ,5 L Water HPLC Plus 1 L ,5 L Ethaol HPLC Plus 1 L ,5 L Isopropaol HPLC Plus 1 L ,5 L Methaol HPLC - Gold 2,5 L L HPLC Plus Gradiet 1 L ,5 L
7 Solvets for HPLC gradiet Comparative table Specificatios Characteristics Uits Acetoitrile Methaol LC MS GOLD HPLC Plus GOLD HPLC Plus Ultragradiet Gradiet LC MS Ultragradiet Gradiet Purity (GC) % mi mi 99.9 mi 99.9 mi mi 99.9 mi 99.9 Water cotet % max 0,01 max 0,01 max 0,01 max 0.02 max 0.02 max 0.02 No volatil residue ppm max 2 max 2 max 2 max 2 max 5 max 5 Acidity meq/g max 0,0005 max 0,0003 max 0,0008 max 0,0003 max 0,0003 max 0,0005 Titrable base meq/g max 0,0002 max 0,0002 max 0,0002 max 0,00006 max 0,00006 max 0,0002 Absorbace at 190 m A.U. max 0,6 max 1,00 at 200 m A.U. max 0,03 max 0,05 at 220 m A.U. max 0,007 max 0,05 at 254 m A.U. max 0,005 max 0,01 Trasmissio UV (1cm - Réf : eau) at 195 m % mi 80 mi 80 mi 79 at 197 m % at 200 m % mi 95 mi 95 mi 90 at 210 m % mi 95 mi 30 mi 30 mi 30 at 220 m % mi 98 mi 98 mi 55 mi 50 at 225 m % mi 99 mi 65 mi 65 from 230 m % mi 99 mi 99 at 235 m % mi 85 mi 85 mi 80 at 240 m % mi 90 at 250 m % mi 95 mi 95 From 255 % mi 98 from 260 m % mi 98 mi 98 mi 98 Fluorescece (quiie) at 254 m ppb max 1 max 1 max 1 max 1 max 1 max 1 Fluorescece(quiie) a 365 m ppb max 0.5 max 0.5 max 0.5 max 1 max 1 max 1 Fluorescece (quiie) at 450 m ppb max 0.5 max 0.5 HPLC gradiet test (1) - Coform Coform Coform Coform Coform Coform HPLC gradiet (1) at 210 m mau max 1 max 1 max 2 at 235 m mau max 2 max 2 max 2 at 254 m mau max 0.2 max 0.2 max 0,8 max 1 max 1 max 1 Drift at 210 m mau max 12 (1) Gradiet test : biary gradiet H2O/acetoitrile from 5 to 90% i acetoitrile for 16 miutes at 1.8 ml/mi LC/MS test TIC ( m/z) ESI (+) Sesitive impurities (i reserpie) ppb max 50 max 50 Metal cotet (2) : Alumiium ppb max 50 max 50 Iro ppb max 50 max 50 Sodium ppb max 50 max 50 Calcium ppb max 50 max 50 Magesium ppb max 50 max 50 Potassium ppb max 50 max 50 (2) Cocetratios measured at batch release
8 Solvets for HPLC isocratic Our solvets for HPLC combie chemical purity with optimal optical trasmissio. Available i glass bottles (1L ad 2.5L) or stailess steel shuttle drums (5 to 1000L), their characteristics satisfy the requiremets of the most advaced HPLC techiques. Product Pkg Code Acetic Acid 1 L ,5 L Acetoe 1 L ,5 L Acetoitrile 1 L ,5 L Butaol 1 L Chlorobutae 1 L Chloroform stabilized with amylee 1 L ,5 L Chloroform stabilized with ethaol 1 L ,5 L Cyclohexae 1 L ,5 L Dichloromethae stabilized with amylee 1 L ,5 L Dichloromethae stabilized with ethaol 1 L ,5 L Ethaol absolute ahydrous 2,5 L Ethyl ether 1 L ,5 L Ethyle acetate 1 L ,5 L Heptae 1 L ,5 L Hexae 1 L ,5 L Hexae 99 % 1 L ,5 L Isohexae 2,5 L Isooctae 1 L ,5 L Methaol 1 L ,5 L Propaol-1 2,5 L Propaol-2 1 L ,5 L Ter-butylmethylether 1 L ,5 L Tetrahydrofura 1 L ,5 L Toluee 1 L ,5 L Triethyl Ami 1 L ,5 L
9 Solvets for preparative HPLC Our rage of solvets for HPLC preparative have bee desiged to satisfy the requiremets of separatios ad purificatios. Their low o volatile residue cotet (from 5 to 10 ppm maximum) allows optimizig the operatio coditios ad to make impurity-free preparatios. All these solvets are available i 2.5 L bottles ad i stailess steel shuttle drums from 5 to L. Product Code 2,5L Acetoitrile Chloroform (stabilized with ethaol) Dichloromethae (stabilized with amylee) Dichloromethae (stabilized with ethaol) Ethyl acetate Propaol Tetrahydrofura Toluee Ter-butylmethylether All our solvets for HPLC ad eluet phases ca be delivered i adapted packagigs: glass bottles of 1 L or 2.5 L, or i stailess steel shuttle drums from 5, 10, 25, 200 litres with samplig systems adapted to your eeds. (special documetatio available o request)
10 Mobile phases If you regularly use a eluet phase, we ca prepare it for you accordig to your specificatios. Your mobile phase is prepared from HPLC quality solvets accordig to procedures (respect of the GMPs) ad with validated equipmet i compliace with the pharmacopeia. The ready-to-use eluet phase esures you: Importat time savig for preparatio Reductio of risks liked to the hadlig of toxic or hazardous products Guarateed ph Possibility of large sized homogeeous batches Labelig coformig to legislatio ad to BPLs It is supplied with: Certificate of aalysis of the batch Safety data sheet Examples Acetoitrile + methaol + buffered ph 5 Acetoitrile + water Ethyl acetate + toluee Water + TFA Water + THF Bacth umber of the mix Expiry date Compositio Specificatios defied by customer Date
11 Io pair reagets The separatio of ioic ad highly polar substaces o reversed phases HPLC colums is facilitated by io pair reagets. Their purity ad UV absorptio test are very importat as mobile phase additives. CARLO ERBA Reagets proposes a rage of most used io pair reagets. Product Code Sodium 1-Heptaesulfoate 25 g Sodium 1-hexaesulfoate 25 g Sodium 1-octaesulphoate 25 g Sodium 1-petaesulphoate 25 g Sodium dodecylbezeesulfoate 10 g Sodium p-tolueesulfoate 100 g Tetrabutylammoium hydrogesulfate 250 g Trimethylcetylammoium bromide 50 g
12 HPLC CER HPLC 2013/01 ALL PICTURES AND SPECIFICATIONS ARE PURELY INDICATIVE AND MAY VARY WITHOUT NOTICE. ITALY CARLO ERBA Reagets S.r.l. VIA R. MERENDI, CORNAREDO (MI) TEL FAX FRANCE CARLO ERBA Reagets SAS CHAUSSÉE DU VEXIN VAL DE REUIL TEL FAX SPAIN CARLO ERBA Reagets S.A. CALLE FILADORS SABADELL TEL FAX
HPLC Solvents, mobile phases and accessories
HPLC Solvets, mobile phases ad accessories CARLO ERBA Reagets Chemicals, Labware ad Life Scieces for Laboratories ad Idustry CARLO ERBA Reagets, a emiet brad with a wellkow history, operates at a Iteratioal
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