UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs

Size: px
Start display at page:

Download "UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs"

Transcription

1 Application Note: 439 UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela UHPLC System MSQ Plus MS Detector Drugs of Abuse Hypersil GOLD PFP Columns Sensitivity Goal Optimize a UHPLC/MS method with respect to stationary phase, mobile phase, and detector settings to achieve picogram level quantitation of fourteen drugs and metabolites employing a 12 minutes separation. Introduction Gas chromatography-mass spectrometry (GC-MS) is commonly employed for the separation and identification of drugs and metabolites in forensic toxicology, using electron impact (EI) or chemical ionization (CI). 1 This methodology has become a gold standard in terms of admissibility and defensibility in court because of its good sensitivity, excellent selectivity and a high degree of standardization. 2 However, laborious and time consuming derivatization procedures and sample clean ups are mandatory in most cases. LC/MS methods eliminate the need to derivatize and often simplify sample preparation. However, long run times and low separation efficiency limit the utility of conventional HPLC. Ultra high performance liquid chromatography (UHPLC) performs separations 5 to 10 times faster than conventional HPLC by employing sub-2 µm diameter particles. The 1-2 second peak widths and relatively high separation efficiency of UHPLC are more competitive with capillary GC, making UHPLC-MS an attractive alternative method for illicit drug analysis. This application note illustrates the separation and detection of a mixture of 14 illicit drugs/metabolites by ultra high performance liquid chromatography-mass spectrometry (UHPLC-MS). The drugs/metabolites are separated on a Hypersil GOLD PFP, 1.9 µm, 100 x 2.1 mm column and detected by a fast scanning single quadrupole mass spectrometer. Experimental Conditions 1. Drug Standard Preparation Pseudoephedrine, ephedrine, amphetamine, methamphetamine, 3,4-methylenedioxy-N-methamphetamine (3,4-MDMA), oxycodone, hydrocodone, clonazepam, noscapine, cocaine, caffeine, tetrahydrocannabinol (THC), cannabinol and cannabidiol standards (1 mg/ml in methanol) were purchased from Alltech-Applied Science (State College, PA, USA). The above fourteen compounds were mixed with the optimized molar ratio in the range of 1 to 100 and diluted to 0.1 ppm with methanol to make the drug mixture standards. 2. Chromatographic Conditions Chromatographic analyses were performed using the Accela UHPLC system (Thermo Scientific, San Jose, CA). The chromatographic conditions were as follows: LC Column: Hypersil GOLD, 1.9 µm, 20 x 2.1 mm Hypersil GOLD, 1.9 µm, 50 x 2.1 mm Hypersil GOLD, 1.9 µm, 100 x 2.1 mm Hypersil GOLD, aq (polar endcapped C18), 1.9 µm, 100 x 2.1 mm Hypersil GOLD PFP (perfluorinated phenyl), 1.9 µm, 100 x 2.1 mm Hypersil GOLD PFP (perfluorinated phenyl), 1.9 µm, 50 x 2.1 mm Column Temperature: 45 C Injection: 1 µl Partial Loop Injection, 25 µl Loop Size Syringe Speed: 8 µl/sec Flush Speed: 100 µl/sec Flush Volume: 400 µl Wash Volume: 100 µl Flush/Wash Source: Bottle with methanol Gradients: Method I Column: Hypersil GOLD PFP 1.9 µm, 100 x 2.1 mm A: Water (0.06% acetic acid) B: Acetonitrile (0.06% acetic acid) C: Methanol (0.06% acetic acid) Time (min) Eluent A% Eluent B% Eluent C% For the other gradient methods used, see Appendix A for details.

2 3. Mass Spectrometer Conditions MS analysis was carried out on a MSQ Plus single quadrupole LC-MS detector (Thermo Scientific, San Jose, CA). The MS conditions were as follows: Ionization: Electrospray (ESI) Polarity: Positive Probe Temperature: 450 C Cone Voltage: 60 V Scan Mode: Full scans ( m/z ) and/or Selected ion monitoring (SIM) ESI Voltage: 4.5 kv Results and Discussion 1. MS Detection Both positive and negative electrospray analysis were performed using the polarity switch function of the Xcalibur software. All of the analytes exhibited higher ionization efficiency in the positive ion mode compared with the negative mode. The MS spectra of the drug standards show both molecular ion signals of [M+H] + and acetonitrile adducts of the form [M+ACN+H] +. For 13 of the analytes, the signal from the molecular ion was more intense than the signal from the acetonitrile adduct. For amphetamine, the most intense signal was from the acetonitrile adduct [M+ACN+H] + at m/z of (data not shown). 2. Separations with Standard Stationary Phases Three columns were evaluated to separate the illicit drug mixtures: Hypersil GOLD, Hypersil GOLD aq and Hypersil GOLD PFP (Figure 1). The UHPLC method with each column type was optimized individually. Hypersil GOLD aq, a polar endcapped C18 phase which offers more retention of polar compounds, did not resolve the early eluting compounds including methamphetamine, oxycodone, caffeine, MDMA and hydrocodone. 3 The separation on Hypersil GOLD aq may have been impaired by interactions between the polar endcapped stationary phase and the polar analytes. Hypersil GOLD, with LI or C18 selectivity, showed improved selectivity for all analytes except caffeine (peak 1) and oxycodone (peak 7). Hypersil GOLD uses highly pure silica and endcapping procedure to minimize unwanted interactions between analytes and the acidic silanols of the silica support. Hypersil GOLD PFP enabled the optimal separation of all 14 analytes by improving the resolution of the earlier eluting compounds. Hypersil GOLD PFP introduces a fluorine group into the stationary phase to improve selectivity towards halogenated compounds, as well as polar compounds containing hydroxyl, carboxyl, nitro or other polar groups Separations using Acetic Acid and Trifluoroacetic Acid (TFA) as Eluent Modifier Trifluoroacetic acid, formic acid and acetic acid can be added into the mobile phase to generate differences in selectivity. Separation of 14 illicit drugs on a Hypersil GOLD PFP column was evaluated by using either trifluoroacetic acid, formic acid or acetic acid as eluent modifier. The separation method with 0.02% TFA (Figure 2A) provided fast separation performance with good resolution and sharp peaks. However, the use of TFA is generally not recommended with MS detection due to its effect on signal suppression. All of the analytes are well resolved with 0.1% formic acid as modifier (Figure 1C), but only when 100% water is used at the beginning of the gradient method (Method C). Prolonged use of 100% water may degrade the stationary phase and shorten the column lifetime, so gradient method C is not suited for routine use. Most of the analytes are well separated with adequate resolution using 0.06% acetic acid as eluent modifier (Figure 2B). However, under such conditions, a few pairs of compounds, such as oxycodone and methamphetamine (peaks 7 & 6), hydrocodone and 3, 4-MDMA (peaks 5 & 8), cocaine and noscapine (peaks 10 & 11), are not baseline resolved. 4. Separations with Hybrid Column Phases Three hybrid stationary phases were evaluated after connecting different stationary phase columns in series: Figure 3A: 50 x 2.1 mm Hypersil GOLD + 50 x 2.1 mm Hypersil GOLD PFP Figure 3B: 50 x 2.1 mm Hypersil GOLD PFP + 50 x 2.1 mm Hypersil GOLD Figure 3C: 100 x 2.1 mm Hypersil GOLD PFP + 20 x 2.1 mm Hypersil GOLD Separations of 14 illicit drugs with these three hybrid stationary phases demonstrated great variation in selectivity. In general, the hybrid column phases improved selectivity between THC and cannabinol, cocaine and noscapine, but reduced selectivity between earlier eluting compounds, such as oxycodone, MA, hydrocodone and MDMA, compared with the Hypersil GOLD PFP phase. 5. Separation with Ternary Gradient The separation of the drug mixtures was dramatically improved by using three solvents: water, acetonitrile and methanol (Figure 4). Baseline resolution of all 14 drugs was achieved. Methanol, a weaker eluent compared with acetonitrile, provided better resolution for most of the analytes. However, the flow rate had to be reduced to accommodate high column backpressure caused by the high viscosity of methanol. Adding acetonitrile reduced the column backpressure so as to maintain the same separation speed.

3 Figure 1: Comparison of 1.9 µm Hypersil GOLD stationary phases for the UHPLC separation of 14 illicit drugs. A) Hypersil GOLD aq, Method A was applied; B) Hypersil GOLD, Method B was applied; C) Hypersil GOLD PFP, Method C was applied. See Appendix A for methods details. 6. Calibration Curve and Sensitivity Calibration curves for the drug standards were constructed over the concentration range listed in Table 1 with 10 calibration levels (Figure 5). Each calibration level was injected three times and the mean area responses were plotted against the concentrations. Correlation coefficients with R 2 = or better were achieved for all illicit drug compounds. The limit of quantitation (LOQ) and the limit of detection (LOD) of the drug compounds were determined based on the calibration curve of signal-to-noise ratio versus concentration and the definitions of LOQ and LOD using s/n = 10 and 3, respectively. LOQs for all drugs were in the range of ng/ml, while LODs were from 0.29 to 90.0 ng/ml (Table 1). The outstanding sensitivity by this method was highlighted by the achievement of picogram level quantitation for 10 illicit drugs with 1 µl sample injection. LOQ LOD Linear Range Analyte (ng/ml) (ng/ml) (ng/ml) ephedrine pseudoephedrine amphetamine methamphetamine ,4-MDMA hydrocodone oxycodone clonazepam cocaine noscapine cannabidiol cannabinol THC Table 1: LOQ and LOD of the thirteen drug compounds with 1 µl sample injection. Figure 2: UHPLC/MS chromatograms of the 14 illicit drugs with acidic solvent modifiers. A) 0.02%TFA (Method D); B) 0.06% acetic acid (Method E). See Appendix A for methods details.

4 Conclusions Fourteen illicit drugs and metabolites are baseline separated in twelve minutes by employing UHPLC/MS with a ternary solvent gradient. Various selectivities are achieved by different column surface chemistry, acidic solvent modifier and eluent system. These results are useful for method developments of drug identification and quantitation. Detection by single quadrupole MS at the ppb (ng/ml) level is more than sufficient to identify and quantify illicit drugs in real samples. References 1. C. Koeppel and J. Tenczer: Scope and limitations of a general unknown screening by gas chromatography-mass spectrometry in acute poisoning. J. Am Soc. Mass Spectrom. 1995, 6, W. Weinmann, A. Wiedemann, B. Eppinger, M. Renz and M. Svoboda: Screening for drugs in serum by electrospray ionization/collisioninduced dissociation and library searching. J. Am Soc Mass Spectrom. 1999, 10, Catalog, Chromatography Columns and Consumables, 08 09, Thermo Scientific, page Figure 3: Comparison of hybrid stationary phase chemistry for the separation of 14 illicit drugs. A) 50 x 2.1 mm Hypersil GOLD + 50 x 2.1 mm Hypersil GOLD PFP, Method F; B) 50 x 2.1 mm Hypersil GOLD PFP + 50 x 2.1 mm Hypersil GOLD, Method G; C) 100 x 2.1 mm Hypersil GOLD PFP + 20 x 2.1 mm Hypersil GOLD, Method H. See Appendix A for method details. Figure 4: Optimized UHPLC/MS separation of 14 illicit drugs with ternary gradient, listed in Method I.

5 Figure 5: Calibration curves for illicit drugs.

6 Appendix A In addition to these offices, Thermo Fisher Method A Method D Method G Scientific maintains Column: Hypersil GOLD aq, 1.9 µm, 100 x 2.1 mm A: Water, 0.1% FA B: Acetonitrile, 0.1% FA Flow Rate: 750 µl/min Method B Column: Hypersil GOLD, 1.9 µm, 100 x 2.1 mm A: Water, 0.1% FA B: Acetonitrile, 0.1% FA Method C Column: Hypersil GOLD PFP, 1.9 µm, 100 x 2.1 mm A: Water, 0.1% FA B: Acetonitrile, 0.1% FA Column: Hypersil GOLD PFP, 1.9 µm, 100 x 2.1mm A: Water, 0.02% TFA B: Acetonitrile, 0.02% TFA Method E Column: Hypersil GOLD PFP, 1.9 µm, 100 x 2.1 mm Method F Hypersil GOLD, 1.9 µm, 50 x 2.1 mm Hypersil GOLD PFP, 1.9 µm, 50 x 2.1 mm Hypersil GOLD PFP, 1.9 µm, 50 x 2.1mm Hypersil GOLD, 1.9 µm, 50 x 2.1 mm Method H Hypersil GOLD PFP, 1.9 µm, 100 x 2.1 mm Hypersil GOLD, 1.9 µm, 20 x 2.1 mm a network of representative organizations throughout the world. Africa Australia Austria Belgium Canada China Denmark Europe-Other France Germany India Italy Japan Latin America Middle East Netherlands South Africa Spain Sweden/Norway/ Finland Switzerland UK USA Legal Notices 2008 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. This information is presented as an example of the capabilities of Thermo Fisher Scientific Inc. products. It is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. View additional Thermo Scientific LC/MS application notes at: Thermo Fisher Scientific, San Jose, CA USA is ISO Certified. AN62904_E 10/08M Part of Thermo Fisher Scientific

Simultaneous Quantitation of 43 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method

Simultaneous Quantitation of 43 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method Simultaneous Quantitation of 4 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method Xiang He and Marta Kozak, Thermo Fisher Scientific, San Jose, CA Application Note 76 Key Words TSQ Quantum Access

More information

An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol

An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol Sarah Fair, Dayana Argoti, Joseph Herman, Thermo Fisher Scientific,

More information

Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection

Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection Derek Hillbeck, Thermo Fisher Scientific, Runcorn, UK Application Note 2999 Key

More information

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs Thermo Scientific Prelude SPLC System FPO Making LC/MS accessible to clinical research and toxicology labs Specifically designed to make the power of LC/MS accessible Analysis of target compounds in complex

More information

LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research

LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research J. Jones, J. Denbigh, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20581 Key Words SPE, SOLA,

More information

Selective Testosterone Analysis in Human Serum by LC-FAIMS-MS/MS

Selective Testosterone Analysis in Human Serum by LC-FAIMS-MS/MS Application Note: 446 Selective Testosterone Analysis in Human Serum by LC-FAIMS-MS/MS Jonathan McNally and Michael Belford, Thermo Fisher Scientific, San Jose, CA James Kapron, Thermo Fisher Scientific,

More information

SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from Human Plasma

SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from Human Plasma SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from uman Plasma Krishna Rao Dara, Dr. Tushar N. Mehta, Asia Pacific Center of Excellence, Thermo Fisher Scientific, Ahmedabad, India Application

More information

Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz

Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz Toxichem Krimtech 211;78(Special Issue):324 Online extraction LC-MS n method for the detection of drugs in urine, serum and heparinized plasma Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische

More information

The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis

The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis Stephen Lock AB SCIEX Warrington (UK) Overview A rapid, robust, sensitive and specific LC-MS/MS method has been developed for

More information

Fast, Reproducible LC-MS/MS Analysis of Dextromethorphan and Dextrorphan

Fast, Reproducible LC-MS/MS Analysis of Dextromethorphan and Dextrorphan Fast, Reproducible LC-MS/MS Analysis of and Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 685 Key Words Accucore C18, dextromethorphan, dextrorphan, SOLA CX Abstract

More information

Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform

Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform Charles Yang, Leo Wang, Dipankar Ghosh, Jonathan Beck,

More information

Analysis of Testosterone in Serum using LC/Triple Quadrupole MS/MS

Analysis of Testosterone in Serum using LC/Triple Quadrupole MS/MS Analysis of Testosterone in Serum using LC/Triple Quadrupole MS/MS Application Note Authors M.P. George Agilent Technologies, Inc. Santa Clara, CA USA Andre Szczesniewski Agilent Technologies, Inc. Schaumburg,

More information

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction Advantages of Using Triple Quadrupole over Single Quadrupole Mass Spectrometry to Quantify and Identify the Presence of Pesticides in Water and Soil Samples André Schreiber AB SCIEX Concord, Ontario (Canada)

More information

Hydrogen Peroxide Detection by Ion Chromatography and Electrochemical Detection

Hydrogen Peroxide Detection by Ion Chromatography and Electrochemical Detection Hydrogen Peroxide Detection by Ion Chromatography and Electrochemical Detection Sheetal Bhardwaj, Rong Lin, Kannan Srinivasan, and Christopher Pohl Thermo Fisher Scientific, Sunnyvale, CA, USA Overview

More information

Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS

Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Stephen Lock 1 and Matthew Noestheden 2 1 AB SCIEX Warrington, Cheshire (UK), 2 AB SCIEX Concord, Ontario (Canada) Overview A rapid,

More information

Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract

Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Lirui Qiao, 1 Rob Lewis, 2 Alex Hooper, 2 James Morphet, 2 Xiaojie Tan, 1 Kate

More information

Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis

Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis Application Note AN844 Extraction of, and from Human Plasma Using EVOLUTE EXPRESS WCX Page 1 Extraction of, and from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis Introduction Catecholamines

More information

AMD Analysis & Technology AG

AMD Analysis & Technology AG AMD Analysis & Technology AG Application Note 120419 Author: Karl-Heinz Maurer APCI-MS Trace Analysis of volatile organic compounds in ambient air A) Introduction Trace analysis of volatile organic compounds

More information

GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert1, Michel Lhermitte 1, Frederic Grisel 2 1

GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert1, Michel Lhermitte 1, Frederic Grisel 2 1 GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert, Michel Lhermitte, Frederic Grisel Laboratoire de Toxicologie & Génopathologie, CHRU Lille, France Waters Corporation, Guyancourt,

More information

Simultaneous qualitative and quantitative analysis using the Agilent 6540 Accurate-Mass Q-TOF

Simultaneous qualitative and quantitative analysis using the Agilent 6540 Accurate-Mass Q-TOF Simultaneous qualitative and quantitative analysis using the Agilent 654 Accurate-Mass Q-TOF Technical Overview Authors Pat Perkins Anabel Fandino Lester Taylor Agilent Technologies, Inc. Santa Clara,

More information

Guide to Reverse Phase SpinColumns Chromatography for Sample Prep

Guide to Reverse Phase SpinColumns Chromatography for Sample Prep Guide to Reverse Phase SpinColumns Chromatography for Sample Prep www.harvardapparatus.com Contents Introduction...2-3 Modes of Separation...4-6 Spin Column Efficiency...7-8 Fast Protein Analysis...9 Specifications...10

More information

# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water

# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water Application Notes # LCMS-35 esquire series Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water An LC-APCI-MS/MS method using an ion trap system

More information

Thermo Scientific SIEVE Software for Differential Expression Analysis

Thermo Scientific SIEVE Software for Differential Expression Analysis m a s s s p e c t r o m e t r y Thermo Scientific SIEVE Software for Differential Expression Analysis Automated, label-free, semi-quantitative analysis of proteins, peptides, and metabolites based on comparisons

More information

Enhancement of Linearity and Response in Charged Aerosol Detection

Enhancement of Linearity and Response in Charged Aerosol Detection Enhancement of Linearity and Response in Charged Aerosol Detection Christopher Crafts, Marc Plante, Bruce Bailey, Ian Acworth, Thermo Fisher Scientific, Chelmsford, MA, USA Overview Purpose: This work

More information

Determination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection

Determination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection Determination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection Application Note 1097 Liu Qian, Zheng Hongguo and

More information

Determination of Ephedra Alkaloids & Synephrine by Strong Cation Exchange SPE and LC MS/MS Detection Using a Pentafluorophenylpropyl HPLC Column

Determination of Ephedra Alkaloids & Synephrine by Strong Cation Exchange SPE and LC MS/MS Detection Using a Pentafluorophenylpropyl HPLC Column Determination of Ephedra Alkaloids & Synephrine by Strong Cation Exchange SPE and LC MS/MS Detection Using a Pentafluorophenylpropyl HPLC Column UCT Part Numbers: CUBCX1HL56: high load benzenesulfonic

More information

Separation and Determination of Liposomal and Non-Liposomal (Free) Doxorubicin from Human Plasma by SPE and LC-MS/MS

Separation and Determination of Liposomal and Non-Liposomal (Free) Doxorubicin from Human Plasma by SPE and LC-MS/MS Separation and Determination of Liposomal and Non-Liposomal (Free) from Human Plasma by SPE and LC-MS/MS Krishna Rao Dara, Dr. Tushar N. Mehta, Tim Liddicoat, Asia Pacific Center of Excellence, Thermo

More information

Thermo Scientific ClinQuan MD Software For In Vitro Diagnostic Use. Confidence in Results With Data Integrity

Thermo Scientific ClinQuan MD Software For In Vitro Diagnostic Use. Confidence in Results With Data Integrity Thermo Scientific ClinQuan MD Software For In Vitro Diagnostic Use Confidence in Results With Data Integrity 2 Make the World Healthier With the LC-MS Tests You Run Confidence in Test Results With Data

More information

New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses

New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses Technical Overview Authors Abstract Peter Stone, Thomas Glauner, Frank Kuhlmann, Tim Schlabach and Ken Miller

More information

Overview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System

Overview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System Investigating the use of the AB SCIEX TripleTOF 4600 LC/MS/MS System for High Throughput Screening of Synthetic Cannabinoids/Metabolites in Human Urine AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS

More information

Pesticide Analysis by Mass Spectrometry

Pesticide Analysis by Mass Spectrometry Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine

More information

Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System

Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System Olaf Scheibner, Maciej Bromirski, Thermo Fisher Scientific, Bremen, Germany

More information

Thermo Scientific HyperSep Solid Phase Extraction Method Development Guide

Thermo Scientific HyperSep Solid Phase Extraction Method Development Guide chromatography Thermo Scientific HyperSep Solid Phase Extraction Method Development Guide The following guide provides considerations, tips and general guidelines for developing SPE methods using the Thermo

More information

IC-MS and LC-MS Determination of Ionic Liquids, Counterions, and Impurities

IC-MS and LC-MS Determination of Ionic Liquids, Counterions, and Impurities ICMS and LCMS Determination of Ionic Liquids, Counterions, and Impurities Leo Wang, Detlef Jensen, and William Schnute Dionex Corporation, Sunnyvale, CA, USA; Dionex Corporation, Olten, Switzerland INTRODUCTION

More information

TargetQuan 3 Software. Leading the way in regulatory. POPs quantification. Bullet Bullet Bullet

TargetQuan 3 Software. Leading the way in regulatory. POPs quantification. Bullet Bullet Bullet TargetQuan 3 Software Leading the way in regulatory POPs quantification Bullet Bullet Bullet Leading the way in regulatory POPs quantification Analyse Samples Open Sequence Process Sequence Analyse Samples

More information

Selective Quantitative Determination of Water Soluble Vitamins in Various Food Matrices Using the ACQUITY UPLC H-Class System and ACQUITY QDa Detector

Selective Quantitative Determination of Water Soluble Vitamins in Various Food Matrices Using the ACQUITY UPLC H-Class System and ACQUITY QDa Detector Selective Quantitative Determination of Water Soluble Vitamins in Various Food Matrices Using the ACQUITY UPLC H-Class System and ACQUITY QDa Detector Mark E. Benvenuti, Dimple Shah, and Jennifer A. Burgess

More information

SCREENING FOR DRUGS IN SERUM AND URINE BY LC/ESI/CID-MS AND MS/MS WITH LIBRARY SEARCHING *

SCREENING FOR DRUGS IN SERUM AND URINE BY LC/ESI/CID-MS AND MS/MS WITH LIBRARY SEARCHING * SCREENING FOR DRUGS IN SERUM AND URINE BY LC/ESI/CID-MS AND MS/MS WITH LIBRARY SEARCHING * Wolfgang WEINMANN 1, Natalie LEHMANN 1, Michaela RENZ 1, Alexander WIEDEMANN 1, Michal SVOBODA 2 1 Institute of

More information

CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY

CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in

More information

A Complete Solution for Method Linearity in HPLC and UHPLC

A Complete Solution for Method Linearity in HPLC and UHPLC Now sold under the Thermo Scientific brand A Complete Solution for Method Linearity in HPLC and UHPLC Frank Steiner, Fraser McLeod, Tobias Fehrenbach, and Andreas Brunner Dionex Corporation, Germering,

More information

Micromass LCT User s Guide

Micromass LCT User s Guide Micromass LCT User s Guide 1) Log on to MassLynx with your username & password. 2) After you have logged in, the MassLynx software will automatically run. 3) After MassLynx has come up, open your project

More information

Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection. No. GCMS No. SSI-GCMS-1403

Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection. No. GCMS No. SSI-GCMS-1403 Gas Chromatograph Mass Spectrometer No. GCMS-1403 Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection Introduction Determination of Blood Alcohol Content (BAC) has been a standard

More information

One Source Toxicology Laboratory, 1213 Genoa Red Bluff, Pasadena, Texas 77504

One Source Toxicology Laboratory, 1213 Genoa Red Bluff, Pasadena, Texas 77504 Validation of Analysis of Amphetamines, Opiates, Phencyclidine, Cocaine, and Benzoylecgonine in Oral Fluids by Liquid Chromatography Tandem Mass Spectrometry Subbarao V. Kala*, Steve E. Harris, Tom D.

More information

Overview. Purpose. Methods. Results

Overview. Purpose. Methods. Results A ovel Approach to Quantify Unbound Cisplatin, Carboplatin, and xaliplatin in Human Plasma Ultrafiltrate by Measuring Platinum-DDTC Complex Using LC/M/M Min Meng, Ryan Kuntz, Al Fontanet, and Patrick K.

More information

Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column

Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column Sachiki Shimizu, FUJIFILM Fine Chemicals Co., Ltd., Kanagawa, Japan Kenneth J. Fountain, Kevin Jenkins, and Yoko Tsuda,

More information

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Hua-Fen Liu, Witold Woroniecki, Doina Caraiman, and Yves LeBlanc AB SCIEX, Foster City, USA One of the most challenging

More information

Ion Chromatography Assay for Chloride and Sulfate in Adenosine

Ion Chromatography Assay for Chloride and Sulfate in Adenosine Ion Chromatography Assay for and in Adenosine Lipika Basumallick and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Application ote 075 Key Words High-Pressure Ion Chromatography, Suppressed

More information

WATERS QUANTITATIVE ANALYSIS solutions

WATERS QUANTITATIVE ANALYSIS solutions More sensitivity. More speed. What more can you ask for in a complete quantitative analysis solution? WATERS QUANTITATIVE ANALYSIS solutions THE CHALLENGE OF QUANTITATIVE ANALYSIS Tandem mass spectrometry,

More information

Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection

Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection APPLICATION NOTE Liquid Chromatography Authors: Catharine Layton Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection Introduction Cannabis

More information

Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping

Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping For Conclusive Characterization of Biologics Deep Protein Characterization Is Crucial Pharmaceuticals have historically been small

More information

Technical Report. Automatic Identification and Semi-quantitative Analysis of Psychotropic Drugs in Serum Using GC/MS Forensic Toxicological Database

Technical Report. Automatic Identification and Semi-quantitative Analysis of Psychotropic Drugs in Serum Using GC/MS Forensic Toxicological Database C146-E175A Technical Report Automatic Identification and Semi-quantitative Analysis of Psychotropic Drugs in Serum Using GC/MS Forensic Toxicological Database Hitoshi Tsuchihashi 1 Abstract: A sample consisting

More information

Thermo Scientific Compound Discoverer Software. A New Generation. of integrated solutions for small molecule structure ID

Thermo Scientific Compound Discoverer Software. A New Generation. of integrated solutions for small molecule structure ID Thermo Scientific Compound Discoverer Software A New Generation of integrated solutions for small molecule structure ID Transforming Small Molecule Research Solutions for Small Molecule Structural ID Thermo

More information

Analyzing Small Molecules by EI and GC-MS. July 2014

Analyzing Small Molecules by EI and GC-MS. July 2014 Analyzing Small Molecules by EI and GC-MS July 2014 Samples Appropriate for GC-MS Volatile and semi-volatile organic compounds Rule of thumb,

More information

Thermo Scientific ExactFinder Software

Thermo Scientific ExactFinder Software mass spectrometry Thermo Scientific ExactFinder Software Workflow software for routine targeted and general unknown screening Unifies qualitative and quantitative high-resolution accurate mass workflows

More information

New drugs of abuse? A case study on Phenazepam & Methoxetamine

New drugs of abuse? A case study on Phenazepam & Methoxetamine New drugs of abuse? A case study on Phenazepam & Methoxetamine Presenter: Nadia Wong Co authors: Dr Yao Yi Ju & Alex Low Xuan Kai Analytical Toxicology Laboratory Clinical & Forensic Toxicology Unit Applied

More information

Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2

Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2 Technical ote Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2 Alek. Dooley, Carmai Seto, esham Ghobarah, and Elliott B. Jones verview:

More information

Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns

Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns Jonathan P. Danaceau, Kenneth J. Fountain, and Erin E. Chambers Waters

More information

Automated Method Development Utilizing Software-Based Optimization and Direct Instrument Control

Automated Method Development Utilizing Software-Based Optimization and Direct Instrument Control Automated Method Development Utilizing Software-Based Optimization and Direct Instrument Control Dr. Frank Steiner, 1 Andreas Brunner, 1 Fraser McLeod, 1 Dr. Sergey Galushko 2 1 Dionex Corporation, Germering,

More information

Thermo Scientific ConFlo IV Universal Interface

Thermo Scientific ConFlo IV Universal Interface m a s s s p e c t r o m e t r y Thermo Scientific ConFlo IV Universal Interface The Universal Interface Part of Thermo Fisher Scientific ConFlo IV Universal Interface The Universal Interface The development

More information

Results of Using Re-washed Vials and Closures

Results of Using Re-washed Vials and Closures Results of Using Rewashed Vials and Closures Loy Shick, Thermo Fisher Scientific, Rockwood, TN, USA Brian King, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20670 Key Words Autosampler,

More information

Joanne Mather, Paul D. Rainville, Kendon S. Graham, and Robert S. Plumb Waters Corporation, Milford, MA, U.S. INTRODUCTION

Joanne Mather, Paul D. Rainville, Kendon S. Graham, and Robert S. Plumb Waters Corporation, Milford, MA, U.S. INTRODUCTION A High Sensitivity UPLC/MS/MS Method for the Analysis of Fluticasone Propionate in Plasma Joanne Mather, Paul D. Rainville, Kendon S. Graham, and Robert S. Plumb Waters Corporation, Milford, MA, U.S. INTRODUCTION

More information

Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography

Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography INTRODUCTION The analysis of soft drinks for caffeine was able to be performed using UV-Vis. The complex sample

More information

[ Care and Use Manual ]

[ Care and Use Manual ] PREP Calibration Mix DIOS Low i. Introduction Pre-packaged PREP Calibration Mixtures eliminate the need to purchase and store large quantities of the component calibration reagents, simplifying sample

More information

Advantages of Polar, Reversed- Phase HPLC Columns for the Analysis of Drug Metabolites

Advantages of Polar, Reversed- Phase HPLC Columns for the Analysis of Drug Metabolites Advantages of Polar, Reversed- Phase HPLC Columns for the Analysis of Drug Metabolites Carmen T. Santasania and David S. Bell SUPELCO, 595 North Harrison Road, Bellefonte, PA 16823 T404045 GUS Abstract

More information

Clinical Chemistry (CHE221) Professor Hicks Lecture 12. Chromatography. chromatography. normal phase chromatography like a tug of war

Clinical Chemistry (CHE221) Professor Hicks Lecture 12. Chromatography. chromatography. normal phase chromatography like a tug of war Clinical Chemistry (CHE221) Professor Hicks Lecture 12 Chromatography chromatography technique to separate mixtures each peak a different substance stationery phase = material mixtures comes in contact

More information

PosterREPRINT AN LC/MS ORTHOGONAL TOF (TIME OF FLIGHT) MASS SPECTROMETER WITH INCREASED TRANSMISSION, RESOLUTION, AND DYNAMIC RANGE OVERVIEW

PosterREPRINT AN LC/MS ORTHOGONAL TOF (TIME OF FLIGHT) MASS SPECTROMETER WITH INCREASED TRANSMISSION, RESOLUTION, AND DYNAMIC RANGE OVERVIEW OVERVIEW Exact mass LC/MS analysis using an orthogonal acceleration time of flight (oa-tof) mass spectrometer is a well-established technique with a broad range of applications. These include elemental

More information

STANFORD UNIVERSITY MASS SPECTROMETRY 333 CAMPUS DR., MUDD 175 STANFORD, CA 94305-5080

STANFORD UNIVERSITY MASS SPECTROMETRY 333 CAMPUS DR., MUDD 175 STANFORD, CA 94305-5080 Training on the ZQ Open access MS Questions? Contact Dr. Allis Chien allis@stanford.edu 650-723-0710 0710 STANFORD UNIVERSITY MASS SPECTROMETRY STANFORD UNIVERSITY MASS SPECTROMETRY 333 CAMPUS DR., MUDD

More information

Third Party Instrument Control with Chromeleon Chromatography Data System (CDS) Software

Third Party Instrument Control with Chromeleon Chromatography Data System (CDS) Software Third Party Instrument Control with Chromeleon Chromatography Data System (CDS) Software White Paper 70885 Executive Summary Over the last few decades chromatographic instrumentation and separations have

More information

LC-MS/MS for Chromatographers

LC-MS/MS for Chromatographers LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS,

More information

Comparison of the Reversed-Phase Selectivity of Solid Core HPLC Columns

Comparison of the Reversed-Phase Selectivity of Solid Core HPLC Columns omparison of the Reversed-Phase Selectivity of Solid ore HPL olumns M. Dolci, L. Pereira, Thermo Fisher Scientific, Runcorn, heshire, UK Technical Note 20788 Key Words Accucore, solid core, column characterization,

More information

Supplementary Materials and Methods (Metabolomics analysis)

Supplementary Materials and Methods (Metabolomics analysis) Supplementary Materials and Methods (Metabolomics analysis) Metabolite extraction and mass spectrometry analysis. 12 Vldlr-/- knock out and 12 wild type (WT) retinas were separately frozen at -80 ºC in

More information

MEPS - Micro Extraction by Packed Sorbent Online SPE for GC and LC sample preparation - Extraction to injection in a single process

MEPS - Micro Extraction by Packed Sorbent Online SPE for GC and LC sample preparation - Extraction to injection in a single process - Micro Extraction by Packed Sorbent Online SPE for GC and LC sample preparation - Extraction to injection in a single process Save Hours in Sample Preparation Reduce the time to prepare and inject samples

More information

Electrospray Ion Trap Mass Spectrometry. Introduction

Electrospray Ion Trap Mass Spectrometry. Introduction Electrospray Ion Source Electrospray Ion Trap Mass Spectrometry Introduction The key to using MS for solutions is the ability to transfer your analytes into the vacuum of the mass spectrometer as ionic

More information

SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC

SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC Katja SÄRKKÄ, Kari ARINIEMI, Pirjo LILLSUNDE Laboratory of Substance Abuse, National Public Health Institute Manerheimintie,

More information

Cliquid Drug Screen & Quant Software for Routine Forensic Toxicology. great interest in forensic, toxicological and clinical research laboratories.

Cliquid Drug Screen & Quant Software for Routine Forensic Toxicology. great interest in forensic, toxicological and clinical research laboratories. Application Note Cliquid Drug Screen & Quant Software A Fast and Sensitive LC/MS/MS Method for the Quantitation and Confirmation of 30 Benzodiazepines and Nonbenzodiazepine Hypnotics in Forensic Urine

More information

Therapeutic Drug Monitoring of Antiretroviral Drugs with HPLC-MS

Therapeutic Drug Monitoring of Antiretroviral Drugs with HPLC-MS Therapeutic Drug Monitoring of Antiretroviral Drugs with PLC-M Ursula Gutteck-Amsler, Katharina M. Rentsch Abstract Prospective and retrospective studies have provided some evidence of the clinical and

More information

Thermo Scientific Syncronis HPLC Columns. Technical Manual

Thermo Scientific Syncronis HPLC Columns. Technical Manual Thermo Scientific Syncronis HPLC Columns Technical Manual Thermo Scientific Syncronis HPLC Columns When developing a new method, one of the most important goals for the chromatographer is to achieve a

More information

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications Introduction The resolving power of chromatography combined with the sensitivity and selectivity

More information

FT-NIR for Online Analysis in Polyol Production

FT-NIR for Online Analysis in Polyol Production Application Note: 51594 FT-NIR for Online Analysis in Polyol Production Key Words Acid Number Ethylene Oxide FT-NIR Hydroxyl Value Polyester Polyols Abstract Hydroxyl value and other related parameters

More information

for mass spectrometry calibration tools Thermo Scientific Pierce Controls and Standards for Mass Spectrometry

for mass spectrometry calibration tools Thermo Scientific Pierce Controls and Standards for Mass Spectrometry Thermo Scientific Pierce Controls and Standards for Mass Spectrometry calibration tools for mass spectrometry Ensure confidence in instrument performance with Thermo Scientific Pierce Calibration Solutions

More information

GC Column Installation, Conditioning, Storage, and Maintenance

GC Column Installation, Conditioning, Storage, and Maintenance GC Column Installation, Conditioning, Storage, and Maintenance Peter Morgan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20684 Key Words Column, installation, conditioning, storage,

More information

Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection

Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection Jeff Layne, Michael McCoy, and Sky Countryman Phenomenex, Inc., 411 Madrid Ave.,

More information

A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System

A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System Kent Johnson Fortes lab, Wilsonville Oregon List of drug of abuse candidates for

More information

Thermo Scientific SOLA SPE cartridges and plates Technical Guide. Join the revolution. unparalleled performance

Thermo Scientific SOLA SPE cartridges and plates Technical Guide. Join the revolution. unparalleled performance Thermo Scientific SOLA SPE cartridges and plates Technical Guide Join the revolution unparalleled performance Join the revolution next-generation SPE Thermo Scientific SOLA products revolutionize Solid

More information

Background Information

Background Information 1 Gas Chromatography/Mass Spectroscopy (GC/MS/MS) Background Information Instructions for the Operation of the Varian CP-3800 Gas Chromatograph/ Varian Saturn 2200 GC/MS/MS See the Cary Eclipse Software

More information

In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates

In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates Using the Explore Workflow on the AB SCIEX TripleTOF 5600 System A major challenge in proteomics

More information

SCREENING FOR THE PRESENCE OF PARA-METHYLTHIOAMPHETAMINE IN URINE BY SOME COMMERCIAL IMMUNOASSAYS AND CONFIRMATION BY GC/MS

SCREENING FOR THE PRESENCE OF PARA-METHYLTHIOAMPHETAMINE IN URINE BY SOME COMMERCIAL IMMUNOASSAYS AND CONFIRMATION BY GC/MS SCREENING FOR THE PRESENCE OF PARA-METHYLTHIOAMPHETAMINE IN URINE BY SOME COMMERCIAL IMMUNOASSAYS AND CONFIRMATION BY GC/MS Ingrid J. BOSMAN 1, Douwe DE BOER 2, Robert A. A. MAES 1 1 Department of Human

More information

Analytical Testing Methods

Analytical Testing Methods Analytical Testing Methods Updated: February 2005 Background The Mandatory Guidelines for Federal Workplace Drug Testing Programs require a laboratory to conduct two analytical tests before a urine specimen

More information

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications TECHNICAL NOTE Toxicology Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications Introduction The resolving power of chromatography combined with

More information

Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow

Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow Abstract There is high demand in clinical research and forensic toxicology for comprehensive,

More information

Mass-Directed Isolation of a Synthetic Peptide Using the ACQUITY QDa Detector

Mass-Directed Isolation of a Synthetic Peptide Using the ACQUITY QDa Detector Mass-Directed Isolation of a Synthetic Peptide Using the ACQUITY QDa Detector Jo-Ann M. Jablonski and Andrew J. Aubin Waters Corporation, Milford, MA, USA APPLICATION BENEFITS The ACQUITY QDa Detector

More information

Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests

Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Application Note: 386 Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Rosa Viner, Terry Zhang, Scott Peterman, and Vlad Zabrouskov, Thermo Fisher Scientific, San Jose, CA,

More information

Comprehensive LC/MS Analysis of Illicit and Pain Management Drugs, Including Their Metabolites, in Urine

Comprehensive LC/MS Analysis of Illicit and Pain Management Drugs, Including Their Metabolites, in Urine Comprehensive LC/MS Analysis of Illicit and Pain Management Drugs, Including Their Metabolites, in Urine Application Note Authors Peter JW Stone and Kevin McCann Agilent Technologies, Inc. Santa Clara,

More information

Ultra Fast UHPLC-LCMSMS Method Development in Clinical Drug Monitoring

Ultra Fast UHPLC-LCMSMS Method Development in Clinical Drug Monitoring PO-CON1359E Ultra Fast UHPLC-LCMSMS Method Development in HPLC 2013 MASS-09 Anja Grüning 1 ; Brigitte Richrath 1 ; Klaus Bollig 2 ; Sven Vedder 1 ; Robert Ludwig 1 1 Shimadzu Europa GmbH, Duisburg, Germany;

More information

Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples

Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Keywords Application Note ENV0212 This collaboration study was performed

More information

Analysis of Polyphenols in Fruit Juices Using ACQUITY UPLC H-Class with UV and MS Detection

Analysis of Polyphenols in Fruit Juices Using ACQUITY UPLC H-Class with UV and MS Detection Analysis of Polyphenols in Fruit Juices Using ACQUITY UPLC H-Class with UV and MS Detection Evelyn Goh, Antonietta Gledhill Waters Pacific, Singapore, Waters Corporation, Manchester, UK A P P L I C AT

More information

A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS

A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS AB SCIEX QTRAP 4500 LC/MS/MS System and Gerstel, Inc. MultiPurpose Sampler

More information

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay PO-CON1484E Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching ASMS 014 TP85 David R. Baker 1, John Warrander 1, Neil Loftus 1, Simon Hird

More information

UPLC-MS/MS Analysis of Aldosterone in Plasma for Clinical Research

UPLC-MS/MS Analysis of Aldosterone in Plasma for Clinical Research UPLC-MS/MS Analysis of in Plasma for Clinical Research Dominic Foley and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION BENEFITS Analytical selectivity improves reproducibility through removal

More information