Depletion Respiration

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1 Depletion Respiration Depletion The investigation and monitoring of biological cleaning treatment processes are becoming more and more important with respect to the environmental requirements such as wastewater treatment, soil remediation and waste treatment. Biological tests are often in the foreground as well as the usual physicalchemical measuring methods. In order to determine the biodegradability of foodstuffs, pollutants, harmful substances or waste substances and the microbial activity so-called respiration (depletion) measurements are often carried out. In these measurements the respiration of the organisms is determined under defined conditions as the oxygen uptake or release of carbon dioxide. Soil respiration Soil analysis: biodegradation of contaminated sites Biodegradation according to OECD Biogas determination Anaerobic degradation Microbiology Growth and stress investigations: determination of respiration rate 130 Modular system for respiration measurements Selected accessories Favorable package price Literature Technical Data: Further information on this subject can be requested from WTW: OxiTop -C measuring head and OxiTop Control OC 110 see page 120 Stirrer platforms see page 124 and 135 Application reports Offprints

2 Laboratory & Field Determination of soil respiration General Laboratory method for determining the microbial soil respiration according to DIN Soil respiration measurements are used for forecasting, surveying and checking remediation work, for biodegradability measurements of substances (pesticides, fungicides, fertilizers, etc.) as well as for carrying out toxicity tests. This determination is possible with the OxiTop Control System and special, practically-tested measuring vessels in a very accurate, simple and favorably-priced way. The expenditure for personnel and apparatus is considerably reduced when compared with conventional methods. Example of application using PF/45... sample vessels Example of application using MG/... measuring vessels Soil respiration measurements can be carried out in 2 different types of vessel. For actively respiring soils with strong CO 2 development the MG 1.0 measuring vessel is recommended: its large opening (approx. 100 mm dia.) means that it can be easily used with large-volume CO 2 absorber vessels for quantitative CO 2 determination. For the determination of aerobic soil respiration optimized application-oriented complete packages are available. Equipment 6 OxiTop -C measuring heads 6 MG 1,0 measuring vessels, 1000 ml with stopper adapters for OxiTop -C alternatively: 6 PF 45/500 sample bottles, 500 ml, Duran, with 6 OxiTop AD/SK adapters, autoclavable for further data processing and documentation of the measuring data 1 Interface cable AK 540 B ISE ORP ph Oxygen (D.O.) Respiration BOD Multi- Parameter Sample Preparation Colony Counter Conductivity Photometry Ordering Information for soil respiration Model Complete soil respiration package Order No. OxiTop Control B6M Package for soil respiration (aerobic) with MG 1.0 measuring vessels, 1000 ml, with stopper adapters for OxiTop -C OxiTop Control B6 Package for soil respiration (aerobic) with PF 45/500 sample vessels, 500 ml, Duran and 6 OxiTop AD/SK adapters, autoclavable Turbidity Software, Printers Customer Service 131

3 OECD OECD complete package A6 package: A12 package: 1 IS 6-Var stirrer platform IS 12 stirrer platform A6 measuring place: A12 measuring place: PF45/1000 PF45/250 If BOD sample bottles are used: S6 package: S12 package: 1 IS 6 stirrer platform IS 12 stirrer platform S6 measuring place: S12 measuring place: PF600 PF600 1 GK 600 L rubber sleeve Determination of biodegradability according to DIN EN / ISO 9408 / OECD 301 F The determination of the biodegradability should be checked before new chemicals are used for the first time, not only for environmental reasons but also to minimize disposal charges. The sample prepared according to the instructions and a blank are stirred at a constant temperature for 28 days in closed bottles. The CO 2 produced is removed from the gas space by means of an absorber so that the resulting negative pressure is a measure of the biodegradability. The continuous recording of the values in the OxiTop -C means that the required documentation can be guaranteed in an optimal manner. The measuring bottles and adapters can be autoclaved at 121 C. Ordering Information for OECD 132 Model Complete OECD packages Order No. OxiTop Control A6 Package for aerobic applications with 6 x 1000 ml measuring places OxiTop Control A12 Package for aerobic applications with 12 x 250 ml measuring places OxiTop Control S6 Package for aerobic applications with 6 x 510 ml measuring places OxiTop Control S12 Package for aerobic applications with 12 x 510 ml measuring places

4 Laboratory & Field General Biogas determination Determination of anaerobic degradation processes: biogas determination Anaerobic degradation processes take place in the absence of oxygen. In order to be able to fill the gas space above the sample with inert gas the measuring bottle has side-mounted connections which are sealed with septa. When anaerobic degradation has taken place the dissolved CO 2 can be driven off and then removed from the gas space by Ordering Information means of a CO 2 absorber. The resulting pressure difference is proportional to the CO 2 concentration, the remaining overpressure is proportional to the methane concentration. The degradation process can be comfortably followed in the pressure operating mode. Model Complete packages for biogas determination Order No. OxiTop Control AN6 Package for aerobic or anaerobic applications with 6 x 1000 ml measuring places OxiTop Control AN12 Package for aerobic or anaerobic applications with 12 x 250 ml measuring places Complete packages for biogas determination AN6 package: 1 Controller OC 110 with operating instructions 1 PC communication program Achat OC 1 IS 6-Var stirrer platform with operating instructions and 230 V plug-in power supply 1 NHP 600 (1 bottle of NaOH pellets) AN6 measuring place: 1 Measuring bottle MF45/1000 AN12 package: 1 Controller OC 110 with operating instructions 1 PC communication program Achat OC 1 IS 12 stirrer platform with operating instructions and 230 V plug-in power supply 1 NHP 600 (1 bottle of NaOH pellets) AN12 measuring place: 1 Measuring bottle MF45/250 ISE ORP ph Oxygen (D.O.) Respiration BOD Multi- Parameter Sample Preparation Colony Counter Conductivity Photometry Turbidity Software, Printers Customer Service 133

5 Microbiology Complete packages for microbiology AN6 package: AN12 package: 1 IS 6-Var stirrer platform IS 12 stirrer platform AN6 AN12 measuring place: measuring place: MF45/1000 MF45/250 Complete packages for aerobic measurements A6 package: A12 package: 1 IS 6-Var stirrer platform IS 12 stirrer platform Microbiological growth and stress investigations: determination of the respiration rate The use of special measuring bottles with side-mounted connections and septum closures allows the interference-free addition of substrates and solutions. As pressure alterations could indicate a reduction in oxygen concentration, for example, which could make the addition of oxygen or air necessary (possibly other gases as well) it is possible to set a warning pressure or a pressure limit so that the operator can carry out manipulations on the system. The momentary pressure can be stored so that the manipulation is fully documented. A6 measuring place: A12 measuring place: PF45/1000 PF45/250 Ordering Information for microbiology Model Complete packages for microbiology Order No. OxiTop Control AN6 Package for aerobic or anaerobic applications with 6 x 1000 ml measuring places OxiTop Control AN12 Package for aerobic or anaerobic applications with 12 x 250 ml measuring places Complete packages for aerobic measurements OxiTop Control A6 Package for aerobic applications with 6 x 1000 ml measuring places OxiTop Control A12 Package for aerobic applications with 12 x 250 ml measuring places

6 Laboratory & Field IS 6-Var Stirrer platform General IS 6-Var Stirrer platform The IS 6-Var Stirrer platform has 6 stirrer positions on the base area of the IS 12 Stirrer platform. This means that sample bottles with a larger base diameter, e.g. MF45/500, MF45/1000 or PF45/500 and PF45/1000 can be stirred. The technical specifications are otherwise the same as for the IS 12 Stirrer platform (see page 124). ISE ORP ph Ordering Information for accessories and spare parts Model Order No. IS 6-Var Stirrer for 6 stirrer positions for sample bottles with a larger base diameter mains operation 230 V/50/60 Hz. Soil respiration accessories / Spare parts MG 1.0 Measuring vessel MG 1.0, 1000 ml, complete for OxiTop -C, consisting of x DV/MG, 4 x DV/MG/KL, 1 x MB50 and 2 x GK 600 L MG 1.5 Measuring vessel MG 1.5, 1500 ml with stopper adapter for OxiTop -C, and x DV/MG, 4 x DV/MG/KL, 1 x MB50 and 2 x GK 600 L MG/ 1.0/E Measuring vessel MG 1.0, 1000 ml, minimum order 6 pieces MG/ 1.5/E Measuring vessel MG 1.5, 1500 ml, minimum order 6 pieces DV/MG Stopper adapter for measuring vessels MG 1.0 and MG 1.5 with support and seal DV/MG/KL Clip for measuring vessels MG 1.0 and MG 1.5, minimum order 20 pieces DV/MG/DI Seal for measuring vessels MG 1.0 and MG 1.5, minimum order 5 pieces MB50 Beaker, 50 ml, as CO 2 absorber Measuring and sample bottles PF 45/100 Sample bottle, 100 ml, Duran, minimum order 3 pieces PF 45/250 Sample bottle, 250 ml, Duran, minimum order 3 pieces PF 45/500 Sample bottle, 500 ml, Duran, minimum order 3 pieces PF 45/1000 Sample bottle, 1000 ml, Duran, minimum order 3 pieces MF 45/250 Measuring bottle 250 ml (Duran) with 2 connections; complete with accessories x VS25, GK 600 L, 2 x SEP25, 2 x STH25 MF 45/500 Measuring bottle 500 ml (Duran) with 2 connections; complete with accessories x VS25, GK 600 L, 2 x SEP25, 2 x STH25 MF 45/1000 Measuring bottle 1000 ml (Duran) with 2 connections; complete with accessories x VS25, GK 600 L, 2 x SEP25, 2 x STH25 OxiTop AD/SK Adapter OxiTop -AD/SK, autoclavable (121 C), consisting of x OxiTop AD, 1 x OxiTop SK, 1 x DI45, 1 x VS45 and 2 x GK 600 L OxiTop AD Adapter, autoclavable (121 C), with 1 x DI OxiTop SK Screw-on sleeve, autoclavable (121 C) DI 45 Seal for measuring vessels PF 45/... autoclavable (121 C), minimum order 5 pieces SEP 25 Septum for measuring bottle MF45/..., minimum order 3 pieces For BOD measurement with OxiTop... see also BOD measurement section from page 114. Oxygen (D.O.) Respiration BOD Multi- Parameter Sample Preparation Colony Counter Conductivity Photometry Turbidity Software, Printers Customer Service 135

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