Enzyme Pre-Lab. Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab.

Save this PDF as:
Size: px
Start display at page:

Download "Enzyme Pre-Lab. Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab."

Transcription

1 Enzyme Pre-Lab Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab. Background: In this investigation, you will study several factors that effect the activity of enzymes. Enzymes are made up of amino acids and have optimal working conditions. The enzyme you will use is catalase, which is present in most cells and found in high concentrations in liver and blood cells. You will use liver as the source of catalase. Catalase promotes the decomposition of hydrogen peroxide (H 2 O 2 ) in the following reaction: 2H 2 O 2 2H 2 O + O 2 Hydrogen peroxide is formed as a by-product of chemical reactions in cells. It is toxic and would kill cells if not immediately removed or broken down. Read the entire lab before answering the questions. USE COMPLETE SENTENCES!!! 1. In the reaction above, what is the substrate? What are the products? 2. What is the purpose of an enzyme? 3. Define the following: a) Active site: b) Substrate: c) Enzyme-substrate complex: 4. Describe the Lock and Key model. (Use a picture and descriptions) 5. Based on the Lock and Key model, do enzymes get used-up? 6. What are enzymes made of? 7. What are the safety precautions for this lab? 8. Why do we need to use CLEAN test tubes for each part? 9. Briefly describe the purpose of each part of the Enzyme lab. 10. Describe activation energy and how enzymes affect it.

2 Materials: Raw liver Enzyme solution on ice Dropper Graduated cylinder Glass rod Straw Enzyme Lab Boiling water Warm water bath Ice bath 0.1 M sodium hydroxide 0.1 M hydrochloric acid 6 test tubes 13 x 100 mm 3% hydrogen peroxide ph paper forceps test tube rack hot plate test tube holder Safety: Safety goggles Keep lab area CLEAN and ORGANIZED Procedure: RECORD DETAILED LAB RESULTS (temperature change, color change, if bubbles were produced, measure the height of the bubbles, time) Complete each part entirely (including the questions) before continuing PART 1: Mixing an enzyme and substrate 1. Obtain supplies: 2 test tubes and test tube rack. 2. Put 20 drops (1ml) of hydrogen peroxide in one test tube (you will use the other test tube in part 2). (CLEAN ALL SPILLS) 3. Add 5 drops of stock enzyme solution to the peroxide. (BE SURE TO KEEP THE ENZYME SOLUTION ON ICE) Note what happens inside the test tube. 4. Feel the bottom of the test tube and note your observation. 5. Dump solution in the waste container (NOT down the sink) and CLEAN the test tube (use test tube brushes). 6. Answer questions. PART 2: Is an enzyme or substrate changed ( used up ) in reaction? 1. Obtain supplies: Glass stirring rod (make sure it does not roll off the table). 2. Label the test tubes A and B. 3. Put a piece of liver (the size of a rice grain) into the bottom of test tube A, using a glass stirring rod to push it to the bottom of the test tube. 4. Put the tube in a rack and add 40 drops (2 ml) of hydrogen peroxide. Note what happens and record in the table in part Allow the reaction to continue until it stops; stir gently. 6. Pour only the reaction liquid into test tube B, do not transfer over the left over liver pieces use the glass rod to assist you. 7. Put another small piece of liver (the size of a rice grain) into test tube B. Note whether or not a reaction occurs when using the used peroxide (Is it bubbling?). Record your results in the table in part Pour another 2ml (40 drops) of hydrogen peroxide in test tube A to see if the used liver is still reactive. Record your results in table in part 2.

3 9. Dump solution in down the sink, but put the piece of liver in the large trash. CLEAN the test tube (use test tube brushes) 10. Using the results, answer questions. PART 3: Is an enzyme specific for only one substrate? 1. Obtain supplies: straw 2. Put 3 ml of distilled water in a test tube and carefully blow into the water with a straw for 1 minute. Carbon dioxide (CO 2 ) is produced in reactions in living cells. Blowing into the tube will add carbon dioxide to the water which will form carbonic acid. 3. Add a small piece of liver to the test tube to see what effects the enzyme catalase (from the liver) has on carbon dioxide. Record your results in the table in part Dump solution in the waster container (NOT down the sink) and CLEAN the test tube (use test tube brushes) 5. Answer questions. Part 4: Effects of ph on enzyme activity 1. Obtain a total of 6 test tubes, label test tubes A, B, C. Do not label the other 3 2. Add 2 ml of distilled water to test tube A. Gently swirl the test tube. 3. Add 2 ml of hydrochloric acid (HCl) to test tube B. Gently swirl the test tube 4. Add 2 ml of sodium hydroxide (NaOH) to test tube C. Gently swirl the test tube. 5. Add 1 ml of stock enzyme solution to test tubes A, B, C. 6. CLEAN-UP ALL SPILLS. Be sure not to splash any chemicals in your face. 7. Use a small piece of ph paper and tweezers, tilt the test tube slightly to test the ph of test tube A, B and C (use a new piece for each test tube). Record results. 8. To each of the three unmarked tubes add 1 ml of hydrogen peroxide. 9. AT THE SAME TIME, pour the contents of the unmarked tubes into each of the labeled test tubes. 10. Record your results. Note the relative rates of each individual reaction. 11. Dump solution in the waster container (NOT down the sink) and CLEAN the test tube (use test tube brushes) 12. Answer questions. Part 5: Effects of temperature on enzyme activity 1. Using your cleaned test tubes, label three A, B, C. Do not mark the other three. 2. To each of the labeled tubes, add 1 ml of stock enzyme solution (liver catalase). 3. AT THE SAME TIME: put tube A in boiling water bath (100 ºC) USE TEST TUBE HOLDER put tube B in warm water bath (about 37 ºC) USE TEST TUBE HOLDER put tube C in an ice bath (0 ºC) 4. Wait 4 minutes. While you wait, add 1 ml of hydrogen peroxide solution to each of the three unmarked test tubes. 5. After 4 minutes, remove the tubes from their baths and put them in a test tube rack. 6. AT THE SAME TIME, add the contents of the unmarked tubes to each tube. A, B, C. 7. Observe the results and BE SURE TO NOTE THE RATE OF EACH REACTION! Record your results. 8. CLEAN AND RETURN ALL SUPPLIES 9. Answer questions.

4 Name: Period: Enzyme Lab Results Sheet RECORD DETAILED LAB RESULTS (temperature change, color change, if bubbles were produced, measure the height of the bubbles, time) PART 1: Mixing an enzyme and substrate Observation notes: 1. What was the source of the temperature change? 2. What is a possible explanation for the bubbling during the reaction? (What chemical might be being released?) 3. The stock enzyme solution was made by crushing a piece of liver in a small amount of water. This released many molecules of enzyme (catalase) into the water. The reaction mixture in your test tube contained hydrogen peroxide (the substrate for the reaction) and this enzyme catalase. Below are three possible explanations: a) The enzyme, catalase, and the substrate, hydrogen peroxide, react together to form a new product and both changed during the reaction. b) The substrate, hydrogen peroxide, is changed during the reaction, but the enzyme, catalase, is unchanged. c) The enzyme, catalase, is changed during the reaction, but the substrate, hydrogen peroxide, is unchanged. Which of the above explanations do you think is correct? Explain why. PART 2: Is an enzyme or substrate changed ( used up ) in reaction? Step # Tube Enzyme Source Substrate Source Results 4 A Fresh piece of liver 2 ml of peroxide 6 B Fresh piece of liver Used peroxide (A) (reaction liquid from A) 7 A Used liver (A) 2 ml fresh peroxide

5 1. Does the peroxide substrate (used peroxide) change after the reaction? How do you know, use evidence from the lab? 2. Does the enzyme (used liver) change after the reaction? How do you know, use evidence from the lab? 3. If left open in the sunlight, a bottle of hydrogen peroxide would slowly break down into water and oxygen. Under these conditions, it takes a long time for the reaction to occur. On the other hand, when catalase is present, this reaction takes place almost immediately. What does this tell you about the function of an enzyme? PART 3: Is an enzyme specific for only one substrate? Step # Tube Enzyme Source Substrate Source Results 3 - Small piece of liver Carbonic acid Using your data collected in parts 1-3 answer the questions below: 1. Write out the formula for the breakdown of hydrogen peroxide in the cell. 2. What role does catalase play in keeping the cell in a healthy environment? 3. Is this enzyme found only in the liver? (Hint: if you put hydrogen peroxide on a cut finger, it bubbles) 4. Compare your results for the action of the liver catalase on the two substrates (hydrogen peroxide in part 2 and carbonic acid) you have tested so far. Did the enzyme catalase have an effect on the carbonic acid (formed when you blew bubbles into the water)? Based on your results, is an enzyme substrate specific? Explain.

6 Part 4: Effects of ph on enzyme activity Tube Contents ph Results A Distilled water + 1 ml enzyme B HCl + 1 ml enzyme C NaOH + 1 ml enzyme 1. Write out an explanation about the possible effects of ph on enzyme activity. 2. What effect does a high ph (base) have on the enzyme activity? Use evidence to support your answer. 3. What effect does a low ph (acid) have on the enzyme activity? Use evidence to support your answer. 4. What effect does a neutral ph have on the activity of the enzyme? Use evidence to support your answer. 5. Which ph is optimal for this reaction to occur? How does this relate to what we know about the body s normal ph level? Use evidence to support your answer. 6. Graph ph versus enzyme activity rate (slow, medium, fast). Label the acid, base and neutral ph ranges and use an arrow to show the optimum rate and ph level for this reaction. fast Rate of Reaction medium slow ph

7 Part 5: Effects of temperature on enzyme activity Tube Contents Temp Results A 1 ml enzyme + 1 ml 100 ºC hydrogen peroxide B 1 ml enzyme + 1 ml 37 ºC hydrogen peroxide C 1 ml enzyme + 1 ml hydrogen peroxide 0 ºC 1. At which temperature was the optimal for enzyme activity? Use evidence to support your answer. 2. Graph the enzyme activity rate (slow, medium, fast) versus temperature. Use an arrow to point out the area of the graph showing the optimal rate and temperature at which the reaction occurred. fast Rate of Reaction medium slow Temperature ºC 3. What is the effect of the temperature on enzyme activity and how does this relate to the normal body temperature of mammals? Use evidence to support your answer.

Enzyme Lab. DEFINITIONS: 1. Enzyme: 2. Catalase: 3. Catalyze: 4. Hydrolysis: 5. Monomer: 6. Digestion: BACKGROUND INFORMATION

Enzyme Lab. DEFINITIONS: 1. Enzyme: 2. Catalase: 3. Catalyze: 4. Hydrolysis: 5. Monomer: 6. Digestion: BACKGROUND INFORMATION Enzyme Lab DEFINITIONS: 1. Enzyme: 2. Catalase: 3. Catalyze: 4. Hydrolysis: 5. Monomer: 6. Digestion: BACKGROUND INFORMATION Many living tissues contain the enzyme catalase. This enzyme breaks down hydrogen

More information

Catalase Enzyme Lab. Background information

Catalase Enzyme Lab. Background information Catalase Enzyme Lab Background information Liver and other living tissues contain the enzyme catalase. This enzyme breaks down hydrogen peroxide, which is a harmful by-product of the process of cellular

More information

10-ml Graduated cylinder 40 ml 3% Hydrogen peroxide solution (found in stores) Straight-edged razor blade Scissors and Forceps (tweezers)

10-ml Graduated cylinder 40 ml 3% Hydrogen peroxide solution (found in stores) Straight-edged razor blade Scissors and Forceps (tweezers) Name: Class: Date: Objectives * Measure the effects of changes in temperature, ph, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. * Explain how environmental

More information

Enzymes Lab Pre-Lab Exercise

Enzymes Lab Pre-Lab Exercise Pre-Lab Exercise Name 1. For the reaction we are studying in this week s lab: a. What is the name of the enzyme? b. What is the substrate? c. What are the products of the reaction? 2. What is the purpose

More information

Sample Liver Enzyme Lab

Sample Liver Enzyme Lab Sample Liver Enzyme Lab Design Aspect 1: Research Question This lab will be driven by the research question, Do changes in temperature have an effect on the activity of the enzyme catalase? Pearson Baccalaureate:

More information

Honors 227 Fall 2007 Laboratory with Ms. Clark. Enzymes, Reactions, Metabolism and Homeostasis

Honors 227 Fall 2007 Laboratory with Ms. Clark. Enzymes, Reactions, Metabolism and Homeostasis 1 Name: Honors 227 Fall 2007 Laboratory with Ms. Clark Enzymes, Reactions, Metabolism and Homeostasis Background Enzymes, which are comprised of amino acids, are very important macromolecules found in

More information

Name. Lab 3: ENZYMES. In this lab, you ll investigate some of the properties of enzymes.

Name. Lab 3: ENZYMES. In this lab, you ll investigate some of the properties of enzymes. Name Lab 3: ENZYMES In this lab, you ll investigate some of the properties of enzymes. So what are enzymes? Enzymes are large protein molecules (macromolecules) They catalyze or speed up chemical reactions

More information

Catalase. ***You will be working with hot water, acids and bases in this laboratory*** ****Use Extreme Caution!!!****

Catalase. ***You will be working with hot water, acids and bases in this laboratory*** ****Use Extreme Caution!!!**** AP BIOLOGY BIOCHEMISTRY ACTIVITY #9 NAME DATE HOUR CATALASE LAB INTRODUCTION Hydrogen peroxide (H 2 O 2 ) is a poisonous byproduct of metabolism that can damage cells if it is not removed. Catalase is

More information

Enzyme Action: Testing Catalase Activity

Enzyme Action: Testing Catalase Activity Enzyme Action: Testing Catalase Activity Experiment 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities

More information

Enzyme Action: Testing Catalase Activity

Enzyme Action: Testing Catalase Activity Enzyme Action: Testing Catalase Activity Experiment 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities

More information

Catalytic Activity of Enzymes

Catalytic Activity of Enzymes Catalytic Activity of Enzymes Introduction Enzymes are biological molecules that catalyze (speed up) chemical reactions. You could call enzymes the Builders and Do-ers in the cell; without them, life could

More information

Properties of Acids and Bases

Properties of Acids and Bases Lab 22 Properties of Acids and Bases TN Standard 4.2: The student will investigate the characteristics of acids and bases. Have you ever brushed your teeth and then drank a glass of orange juice? What

More information

Enzyme Action: Testing Catalase Activity 50 Points

Enzyme Action: Testing Catalase Activity 50 Points Names: LabQuest Enzyme Action: Testing Catalase Activity 50 Points 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the

More information

THE ACTIVITY OF LACTASE

THE ACTIVITY OF LACTASE THE ACTIVITY OF LACTASE Lab VIS-8 From Juniata College Science in Motion Enzymes are protein molecules which act to catalyze the chemical reactions in living things. These chemical reactions make up the

More information

ENZYME ACTION: TESTING CATALASE ACTIVITY

ENZYME ACTION: TESTING CATALASE ACTIVITY ENZYME ACTION: TESTING CATALASE ACTIVITY LAB ENZ 1.CALC From Biology with Calculators, Vernier Software & Technology, 2000 INTRODUCTION Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically.

More information

Running Head: ACTION OF CATALASE IN DIFFERENT TISSUES 1. Action of Catalase in Different Tissues. San Nguyen. SanNguyen2@my.unt.edu. Biol 1730.

Running Head: ACTION OF CATALASE IN DIFFERENT TISSUES 1. Action of Catalase in Different Tissues. San Nguyen. SanNguyen2@my.unt.edu. Biol 1730. Running Head: ACTION OF CATALASE IN DIFFERENT TISSUES 1 Action of Catalase in Different Tissues San Nguyen SanNguyen2@my.unt.edu Biol 1730.537 October 12, 2012 Aswad Khadilka October 12, 2012; 8:00 am

More information

Evaluation copy. Enzyme Action: Testing Catalase Activity (Method 1 O 2 Gas Sensor) Computer 2

Evaluation copy. Enzyme Action: Testing Catalase Activity (Method 1 O 2 Gas Sensor) Computer 2 Enzyme Action: Testing Catalase Activity (Method 1 O 2 Gas Sensor) Computer 2 Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most

More information

Recovery of Elemental Copper from Copper (II) Nitrate

Recovery of Elemental Copper from Copper (II) Nitrate Recovery of Elemental Copper from Copper (II) Nitrate Objectives: Challenge: Students should be able to - recognize evidence(s) of a chemical change - convert word equations into formula equations - perform

More information

Activity Sheets Enzymes and Their Functions

Activity Sheets Enzymes and Their Functions Name: Date: Activity Sheets Enzymes and Their Functions amylase What are Enzymes? starch glucose Enzymes are compounds that assist chemical reactions by increasing the rate at which they occur. For example,

More information

Enzyme Action: Testing Catalase Activity

Enzyme Action: Testing Catalase Activity Enzyme Action: Testing Catalase Activity DataQuest 12 Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities

More information

PRE-LAB FOR YEAST RESPIRATION AND FERMENTATION

PRE-LAB FOR YEAST RESPIRATION AND FERMENTATION PRE-LAB FOR YEAST RESPIRATION AND FERMENTATION PURPOSE: To identify the products of yeast cultures grown under aerobic and anaerobic conditions STUDENTS' ENTERING COMPETENCIES: Before doing this lab, students

More information

Chem 100 Lab Experiment #9 - ACID/BASE INDICATORS

Chem 100 Lab Experiment #9 - ACID/BASE INDICATORS Lab #9 Chem 100 Lab Experiment #9 - ACID/BASE INDICATORS Name: Purpose: In this laboratory we will investigate how indicators can be used to test for the presence of acids or bases in a number of common

More information

Dissolving of sodium hydroxide generates heat. Take care in handling the dilution container.

Dissolving of sodium hydroxide generates heat. Take care in handling the dilution container. TITRATION: STANDARDIZATION OF A BASE AND ANALYSIS OF STOMACH ANTACID TABLETS 2009, 1996, 1973 by David A. Katz. All rights reserved. Reproduction permitted for education use provided original copyright

More information

Factors Affecting Enzyme Activity

Factors Affecting Enzyme Activity INTRODUCTION Factors Affecting Enzyme Activity The chemical reactions occurring in living things are controlled by enzymes. An enzyme is a protein in the cell which lowers the activation energy of a catalyzed

More information

Amino Acids, Peptides, and Proteins

Amino Acids, Peptides, and Proteins 1 Amino Acids, Peptides, and Proteins Introduction Amino Acids Amino acids are the building blocks of proteins. In class you learned the structures of the 20 common amino acids that make up proteins. All

More information

CHM 130LL: ph, Buffers, and Indicators

CHM 130LL: ph, Buffers, and Indicators CHM 130LL: ph, Buffers, and Indicators Many substances can be classified as acidic or basic. Acidic substances contain hydrogen ions, H +, while basic substances contain hydroxide ions, OH. The relative

More information

Metabolism: Cellular Respiration, Fermentation and Photosynthesis

Metabolism: Cellular Respiration, Fermentation and Photosynthesis Metabolism: Cellular Respiration, Fermentation and Photosynthesis Introduction: All organisms require a supply of energy and matter to build themselves and to continue to function. To get that supply of

More information

catalase 2H 2 O 2 (l) ----> 2H 2 O (l) + O 2 (g)

catalase 2H 2 O 2 (l) ----> 2H 2 O (l) + O 2 (g) ENZYME POST LAB QUIZ STUDY GUIDE Below are the answers to the post-lab (Data Analysis) questions. Make sure you UNDERSTAND all of these questions. The post-lab questions will, of course, be different,

More information

This laboratory explores the affects ph has on a reaction rate. The reaction

This laboratory explores the affects ph has on a reaction rate. The reaction Joy Paul Enzyme Catalyst lab Abstract: This laboratory explores the affects ph has on a reaction rate. The reaction studied was the breakdown of hydrogen peroxide catalyzed by the enzyme peroxidase. Three

More information

Ann.wellhouse@TouchStoneScience.net 1. Enzyme Function

Ann.wellhouse@TouchStoneScience.net 1. Enzyme Function Ann.wellhouse@TouchStoneScience.net 1 Enzyme Function National Science Standards Science as Inquiry: Content Standard A: As a result of activities in grades 9-12, all students should develop: Abilities

More information

Properties of Acids and Bases

Properties of Acids and Bases Properties of Acids and Bases (Adapted from Flinn Scientific Acid Base Test Kit I #AP4567) Introduction Battery acid, stomach acid, acid rain just a few acids in our everyday life! What does it mean when

More information

Physical and Chemical Properties and Changes

Physical and Chemical Properties and Changes Physical and Chemical Properties and Changes An understanding of material things requires an understanding of the physical and chemical characteristics of matter. A few planned experiments can help you

More information

PART I: PREPARATION OF SOLUTIONS AND STANDARDIZATION OF A BASE

PART I: PREPARATION OF SOLUTIONS AND STANDARDIZATION OF A BASE TITRATION: STANDARDIZATION OF A BASE AND ANALYSIS OF STOMACH ANTACID TABLETS 2009, 1996, 1973 by David A. Katz. All rights reserved. Reproduction permitted for education use provided original copyright

More information

Chemical versus Physical Changes

Chemical versus Physical Changes Chemical versus Physical Changes Permission to Copy - This document may be reproduced for non-commercial educational purposes Copyright 2009 General Electric Company What are physical and chemical changes?

More information

2(H 2 O 2 ) catalase 2H 2 O + O 2

2(H 2 O 2 ) catalase 2H 2 O + O 2 Enzyme Model Catalase Student Guide Catalysts accelerate chemical reactions that otherwise proceed slowly. The enzyme called catalase is a catalyst. It exists in plant and animal cells and breaks down

More information

Biopharmaceuticals and Biotechnology Unit 2 Student Handout. DNA Biotechnology and Enzymes

Biopharmaceuticals and Biotechnology Unit 2 Student Handout. DNA Biotechnology and Enzymes DNA Biotechnology and Enzymes 35 Background Unit 2~ Lesson 1 The Biotechnology Industry Biotechnology is a process (or a technology) that is used to create products like medicines by using micro-organisms,

More information

Experiment 8 - Double Displacement Reactions

Experiment 8 - Double Displacement Reactions Experiment 8 - Double Displacement Reactions A double displacement reaction involves two ionic compounds that are dissolved in water. In a double displacement reaction, it appears as though the ions are

More information

Micro Mole Rockets Hydrogen and Oxygen Mole Ratio As adapted from Flinn ChemTopic- Labs - Molar Relationships & Stoichiometry

Micro Mole Rockets Hydrogen and Oxygen Mole Ratio As adapted from Flinn ChemTopic- Labs - Molar Relationships & Stoichiometry Micro Mole Rockets Hydrogen and Oxygen Mole Ratio As adapted from Flinn ChemTopic- Labs - Molar Relationships & Stoichiometry Introduction The combustion reaction of hydrogen and oxygen is used to produce

More information

LAB TOPIC 4: ENZYMES. Enzyme catalyzed reactions can be expressed in the following way:

LAB TOPIC 4: ENZYMES. Enzyme catalyzed reactions can be expressed in the following way: LAB TOPIC 4: ENZYMES Objectives Define enzyme and describe the activity of enzymes in cells. Discuss the effects of varying enzyme concentrations on the rate of enzyme activity. Discuss the effects of

More information

ACIDS AND BASES SAFETY PRECAUTIONS

ACIDS AND BASES SAFETY PRECAUTIONS ACIDS AND BASES Mild acids and bases are used in cooking (their reaction makes biscuits and bread rise). Acids such as those in our stomachs eat away at food or digest it. Strong acids and bases are used

More information

Taking Apart the Pieces

Taking Apart the Pieces Lab 4 Taking Apart the Pieces How does starting your morning out right relate to relief from a headache? I t is a lazy Saturday morning and you ve just awakened to your favorite cereal Morning Trails and

More information

Key Questions. What happens to chemical bonds during chemical reactions?

Key Questions. What happens to chemical bonds during chemical reactions? LESSON 2.4 Getting Started Objectives 2.4.1 Explain how chemical reactions affect chemical bonds. 2.4.2 Describe how energy changes affect how easily a chemical reaction will occur. 2.4.3 Explain why enzymes

More information

Experiment 7: Titration of an Antacid

Experiment 7: Titration of an Antacid 1 Experiment 7: Titration of an Antacid Objective: In this experiment, you will standardize a solution of base using the analytical technique known as titration. Using this standardized solution, you will

More information

STANDARDIZATION OF A SODIUM HYDROXIDE SOLUTION EXPERIMENT 14

STANDARDIZATION OF A SODIUM HYDROXIDE SOLUTION EXPERIMENT 14 STANDARDIZATION OF A SODIUM HYDROXIDE SOLUTION EXPERIMENT 14 OBJECTIVE The objective of this experiment will be the standardization of sodium hydroxide using potassium hydrogen phthalate by the titration

More information

Experiment 10 Enzymes

Experiment 10 Enzymes Experiment 10 Enzymes Enzymes are proteins that act as catalysts for biological reactions. Enzymes, like all catalysts, speed up reactions without being used up themselves. They do this by lowering the

More information

Lab #13: Qualitative Analysis of Cations and Anions

Lab #13: Qualitative Analysis of Cations and Anions Lab #13: Qualitative Analysis of Cations and Anions Objectives: 1. To understand the rationale and the procedure behind the separation for various cations and anions. 2. To perform qualitative analysis

More information

Mixtures and Pure Substances

Mixtures and Pure Substances Unit 2 Mixtures and Pure Substances Matter can be classified into two groups: mixtures and pure substances. Mixtures are the most common form of matter and consist of mixtures of pure substances. They

More information

Santa Monica College Chemistry 11

Santa Monica College Chemistry 11 Types of Reactions Objectives The objectives of this laboratory are as follows: To perform and observe the results of a variety of chemical reactions. To become familiar with the observable signs of chemical

More information

Acid Base Titrations

Acid Base Titrations Acid Base Titrations Introduction A common question chemists have to answer is how much of something is present in a sample or a product. If the product contains an acid or base, this question is usually

More information

Investigation 2- ENZYME ACTIVITY BACKGROUND catalase Learning Objectives

Investigation 2- ENZYME ACTIVITY BACKGROUND catalase Learning Objectives Investigation 2-13 ENZYME ACTIVITY How do abiotic or biotic factors influence the rates of enzymatic reactions? BACKGROUND Enzymes are the catalysts of biological systems. They speed up chemical reactions

More information

Extraction: Separation of Acidic Substances

Extraction: Separation of Acidic Substances Extraction: Separation of Acidic Substances Chemists frequently find it necessary to separate a mixture of compounds by moving a component from one solution or mixture to another. The process most often

More information

1. 4. 1: Biochemistry of macromolecules and metabolic pathways

1. 4. 1: Biochemistry of macromolecules and metabolic pathways 1. 4 Investigating enzymes Many factors affect the activity of enzymes and it is very easy to investigate these factors using common enzymes. Enzymes work at their optimum temperature and ph. Any changes

More information

In this experiment, we will use three properties to identify a liquid substance: solubility, density and boiling point..

In this experiment, we will use three properties to identify a liquid substance: solubility, density and boiling point.. Identification of a Substance by Physical Properties 2009 by David A. Katz. All rights reserved. Permission for academic use provided the original copyright is included Every substance has a unique set

More information

Household Acids and Bases

Household Acids and Bases Household Acids and Bases GRADE LEVEL INDICATORS Experiment Demonstrate that the ph scale (0-14) is used to measure acidity and classify substances or solutions as acidic, basic, or neutral. 21 Develop

More information

Neutralizing an Acid and a Base

Neutralizing an Acid and a Base Balancing Act Teacher Information Objectives In this activity, students neutralize a base with an acid. Students determine the point of neutralization of an acid mixed with a base while they: Recognize

More information

15. Acid-Base Titration. Discover the concentration of an unknown acid solution using acid-base titration.

15. Acid-Base Titration. Discover the concentration of an unknown acid solution using acid-base titration. S HIFT INTO NEUTRAL 15. Acid-Base Titration Shift into Neutral Student Instruction Sheet Challenge Discover the concentration of an unknown acid solution using acid-base titration. Equipment and Materials

More information

Experiment 16-Acids, Bases and ph

Experiment 16-Acids, Bases and ph Definitions acid-an ionic compound that releases or reacts with water to form hydrogen ion (H + ) in aqueous solution. They taste sour and turn litmus red. Acids react with certain metals such as zinc,

More information

Chemical reaction (slow): Enzyme-catalyzed reaction (much faster):

Chemical reaction (slow): Enzyme-catalyzed reaction (much faster): 1 Enzymes Introduction Enzymes are Biological Catalysts Recall that a catalyst is an agent which speeds up a chemical reaction without actually being consumed or changed by the reaction. Enzymes are proteins

More information

Chemistry 112 Laboratory Experiment 6: The Reaction of Aluminum and Zinc with Hydrochloric Acid

Chemistry 112 Laboratory Experiment 6: The Reaction of Aluminum and Zinc with Hydrochloric Acid Chemistry 112 Laboratory Experiment 6: The Reaction of Aluminum and Zinc with Hydrochloric Acid Introduction Many metals react with acids to form hydrogen gas. In this experiment, you will use the reactions

More information

To determine the equivalence points of two titrations from plots of ph versus ml of titrant added.

To determine the equivalence points of two titrations from plots of ph versus ml of titrant added. Titration Curves PURPOSE To determine the equivalence points of two titrations from plots of ph versus ml of titrant added. GOALS 1 To gain experience performing acid-base titrations with a ph meter. 2

More information

Cornell Institute for Biology Teachers

Cornell Institute for Biology Teachers Cornell Institute for Biology Teachers Copyright CIBT This work may be copied by the original recipient from CIBT to provide copies for users working under the direction of the original recipient. All

More information

ACID-BASE TITRATIONS: DETERMINATION OF CARBONATE BY TITRATION WITH HYDROCHLORIC ACID BACKGROUND

ACID-BASE TITRATIONS: DETERMINATION OF CARBONATE BY TITRATION WITH HYDROCHLORIC ACID BACKGROUND #3. Acid - Base Titrations 27 EXPERIMENT 3. ACID-BASE TITRATIONS: DETERMINATION OF CARBONATE BY TITRATION WITH HYDROCHLORIC ACID BACKGROUND Carbonate Equilibria In this experiment a solution of hydrochloric

More information

Name Date Period. Keystone Review Enzymes

Name Date Period. Keystone Review Enzymes Name Date Period Keystone Review Enzymes 1. In order for cells to function properly, the enzymes that they contain must also function properly. What can be inferred using the above information? A. Cells

More information

ph units constitute a scale which allows scientists to determine the acid or base content of a substance or solution. The ph 0

ph units constitute a scale which allows scientists to determine the acid or base content of a substance or solution. The ph 0 ACID-BASE TITRATION LAB PH 2.PALM INTRODUCTION Acids and bases represent a major class of chemical substances. We encounter them every day as we eat, clean our homes and ourselves, and perform many other

More information

How to write a formal lab report correctly. This is based off a lab done in AP biology and all examples are taken from student lab write-ups.

How to write a formal lab report correctly. This is based off a lab done in AP biology and all examples are taken from student lab write-ups. How to write a formal lab report correctly. This is based off a lab done in AP biology and all examples are taken from student lab write-ups. Title: Potato Catalase Enzyme Lab (1 point). * Objective, variables,

More information

Chemistry 212 VAPOR PRESSURE OF WATER LEARNING OBJECTIVES

Chemistry 212 VAPOR PRESSURE OF WATER LEARNING OBJECTIVES Chemistry 212 VAPOR PRESSURE OF WATER LEARNING OBJECTIVES The learning objectives of this experiment are to explore the relationship between the temperature and vapor pressure of water. determine the molar

More information

Enzyme Activity Measuring the Effect of Enzyme Concentration

Enzyme Activity Measuring the Effect of Enzyme Concentration 6 Measuring the Effect of Enzyme Concentration Enzymes are proteins that serve as biological catalysts in a wide variety of life sustaining chemical reactions that take place in cells. As catalysts, enzymes

More information

Laboratory 22: Properties of Alcohols

Laboratory 22: Properties of Alcohols Introduction Alcohols represent and important class of organic molecules. In this experiment you will study the physical and chemical properties of alcohols. Solubility in water, and organic solvents,

More information

Determination of the Amount of Acid Neutralized by an Antacid Tablet Using Back Titration

Determination of the Amount of Acid Neutralized by an Antacid Tablet Using Back Titration Determination of the Amount of Acid Neutralized by an Antacid Tablet Using Back Titration GOAL AND OVERVIEW Antacids are bases that react stoichiometrically with acid. The number of moles of acid that

More information

The Properties of Water (Instruction Sheet)

The Properties of Water (Instruction Sheet) The Properties of Water (Instruction Sheet) Property : High Polarity Activity #1 Surface Tension: PILE IT ON. Materials: 1 DRY penny, 1 eye dropper, water. 1. Make sure the penny is dry. 2. Begin by estimating

More information

Where the exp subscripts refer to the experimental temperature and pressure acquired in the laboratory.

Where the exp subscripts refer to the experimental temperature and pressure acquired in the laboratory. Molar Volume of Carbon Dioxide Reading assignment: Julia Burdge, Chemistry 3rd edition, Chapter 10. Goals To determine the molar volume of carbon dioxide gas and the amount of sodium carbonate in a sample.

More information

PREPARATION FOR CHEMISTRY LAB: COMBUSTION

PREPARATION FOR CHEMISTRY LAB: COMBUSTION 1 Name: Lab Instructor: PREPARATION FOR CHEMISTRY LAB: COMBUSTION 1. What is a hydrocarbon? 2. What products form in the complete combustion of a hydrocarbon? 3. Combustion is an exothermic reaction. What

More information

Enzymes. A. a lipid B. a protein C. a carbohydrate D. a mineral

Enzymes. A. a lipid B. a protein C. a carbohydrate D. a mineral Enzymes 1. All cells in multicellular organisms contain thousands of different kinds of enzymes that are specialized to catalyze different chemical reactions. Given this information, which of the following

More information

The Chemistry of Carbohydrates

The Chemistry of Carbohydrates The Chemistry of Carbohydrates Experiment #5 Objective: To determine the carbohydrate class of an unknown by carrying out a series of chemical reactions with the unknown and known compounds in each class

More information

OBJECTIVES: Visitors learn what an antioxidant is and how it behaves. They also learn how to test for the presence of vitamin C..

OBJECTIVES: Visitors learn what an antioxidant is and how it behaves. They also learn how to test for the presence of vitamin C.. Vitamin C Visitors use iodine to compare the reactivity of two starch solutions one with vitamin C added, one without vitamin C. OBJECTIVES: Visitors learn what an antioxidant is and how it behaves. They

More information

The Huntington Library, Art Collections, and Botanical Gardens. How Sweet It Is: Enzyme Action in Seed Germination

The Huntington Library, Art Collections, and Botanical Gardens. How Sweet It Is: Enzyme Action in Seed Germination The Huntington Library, Art Collections, and Botanical Gardens How Sweet It Is: Enzyme Action in Seed Germination Overview This experiment is intended to familiarize students with the macromolecule starch,

More information

OXIDATION-REDUCTION TITRATIONS-Permanganometry

OXIDATION-REDUCTION TITRATIONS-Permanganometry Experiment No. Date OXIDATION-REDUCTION TITRATIONS-Permanganometry INTRODUCTION Potassium permanganate, KMnO 4, is probably the most widely used of all volumetric oxidizing agents. It is a powerful oxidant

More information

Human Physiology Lab (Biol 236L) Digestive Physiology: Amylase hydrolysis of starch

Human Physiology Lab (Biol 236L) Digestive Physiology: Amylase hydrolysis of starch Human Physiology Lab (Biol 236L) Digestive Physiology: Amylase hydrolysis of starch Introduction Enzymes are proteins composed of amino acid building blocks. Enzymes catalyze or increase the rate of metabolic

More information

Laboratory 5: Properties of Enzymes

Laboratory 5: Properties of Enzymes Laboratory 5: Properties of Enzymes Technical Objectives 1. Accurately measure and transfer solutions with pipettes 2. Use a Spectrophotometer to study enzyme action. 3. Properly graph a set of data. Knowledge

More information

Chapter 6, Lesson 4: Temperature and the Rate of a Chemical Reaction

Chapter 6, Lesson 4: Temperature and the Rate of a Chemical Reaction Chapter 6, Lesson 4: Temperature and the Rate of a Chemical Reaction Key Concepts Reactants must be moving fast enough and hit each other hard enough for a chemical reaction to take place. Increasing the

More information

EXPERIMENT 10: TITRATION AND STANDARDIZATION

EXPERIMENT 10: TITRATION AND STANDARDIZATION EXPERIMENT 10: TITRATION AND STANDARDIZATION PURPOSE To determine the molarity of a NaOH solution by titrating it with a standard HCl solution. To determine the molarity of acetic acid in vinegar using

More information

Physical and Chemical Changes

Physical and Chemical Changes Physical and Chemical Changes Jana Barrow West Point Jr. High 2775 W 550 N 801-402-8100 West Point, UT 84015 jbarrow@dsdmail.net Eighth Grade Integrated Science Standard I: Students will understand the

More information

Determination of a Chemical Formula

Determination of a Chemical Formula 1 Determination of a Chemical Formula Introduction Molar Ratios Elements combine in fixed ratios to form compounds. For example, consider the compound TiCl 4 (titanium chloride). Each molecule of TiCl

More information

Reaction of Magnesium with Hydrochloric Acid (Gas Laws) Chemicals Needed:

Reaction of Magnesium with Hydrochloric Acid (Gas Laws) Chemicals Needed: Reaction of Magnesium with Hydrochloric Acid (Gas Laws) Your Name: Date: Partner(s) Names: Objectives: React magnesium metal with hydrochloric acid, collecting the hydrogen over water. Calculate the grams

More information

Absorption and Transport of Nutrients

Absorption and Transport of Nutrients Page1 Digestion Food travels from mouth esophagus stomach small intestine colon rectum anus. Food mixes with digestive juices, moving it through the digestive tract Large molecules of food are broken into

More information

Properties of Alcohols and Phenols Experiment #3

Properties of Alcohols and Phenols Experiment #3 Properties of Alcohols and Phenols Experiment #3 Objectives: To observe the solubility of alcohols relative to their chemical structure, to perform chemical tests to distinguish primary, secondary and

More information

Chemical Changes. Measuring a Chemical Reaction. Name(s)

Chemical Changes. Measuring a Chemical Reaction. Name(s) Chemical Changes Name(s) In the particle model of matter, individual atoms can be bound tightly to other atoms to form molecules. For example, water molecules are made up of two hydrogen atoms bound to

More information

Hands-On Labs SM-1 Lab Manual

Hands-On Labs SM-1 Lab Manual EXPERIMENT 4: Separation of a Mixture of Solids Read the entire experiment and organize time, materials, and work space before beginning. Remember to review the safety sections and wear goggles when appropriate.

More information

CELLS An Introduction to Cell Structure & Function

CELLS An Introduction to Cell Structure & Function CELLS An Introduction to Cell Structure & Function Introduction: In this lab exercise we will be studying three general aspects of cellular structure and function. First, we will observe the anatomical

More information

Experiment 12- Classification of Matter Experiment

Experiment 12- Classification of Matter Experiment Experiment 12- Classification of Matter Experiment Matter can be classified into two groups: mixtures and pure substances. Mixtures are the most common form of matter and consist of mixtures of pure substances.

More information

Experiment 18: ph Measurements of Common Substances. Experiment 17: Reactions of Acids with Common Substances

Experiment 18: ph Measurements of Common Substances. Experiment 17: Reactions of Acids with Common Substances Experiment 18: ph Measurements of Common Substances and Experiment 17: Reactions of Acids with Common Substances What is this lab about? You mean what ARE THESE labs about? Ok, so what are THESE labs about?

More information

Table of Content. Enzymes and Their Functions Teacher Version 1

Table of Content. Enzymes and Their Functions Teacher Version 1 Enzymes and Their Functions Jeisa Pelet, Cornell University Carolyn Wilczynski, Binghamton High School Cornell Learning Initiative in Medicine and Bioengineering (CLIMB) Table of Content Title Page Abstract..

More information

Class Copy! Class Copy! The process of photosynthesis can be expressed by the following word equation and chemical equation.

Class Copy! Class Copy! The process of photosynthesis can be expressed by the following word equation and chemical equation. Floating Leaf Disk Photosynthesis Lab Introduction: Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. When light is absorbed by pigments in

More information

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy Determining the Quantity of Iron in a Vitamin Tablet Computer 34 As biochemical research becomes more sophisticated, we are learning more about the role of metallic elements in the human body. For example,

More information

Target Mole Lab. Mole Relationships and the Balanced Equation. For each student group Hydrochloric acid solution, HCl, 3 M, 30 ml

Target Mole Lab. Mole Relationships and the Balanced Equation. For each student group Hydrochloric acid solution, HCl, 3 M, 30 ml elearning 2009 Introduction Target Mole Lab Mole Relationships and the Balanced Equation Publication No. A common chemical reaction used in chemistry class is zinc and hydrochloric In this lab, students

More information

Shampoo Properties Evaluation General Science

Shampoo Properties Evaluation General Science / 10 Shampoo Properties Evaluation General Science Name It is difficult to obtain exact information on the formulation of commercial shampoos. These facts are held by the manufacturer to protect their

More information

Figure 5. Energy of activation with and without an enzyme.

Figure 5. Energy of activation with and without an enzyme. Biology 20 Laboratory ENZYMES & CELLULAR RESPIRATION OBJECTIVE To be able to list the general characteristics of enzymes. To study the effects of enzymes on the rate of chemical reactions. To demonstrate

More information

EXPERIMENT 4 Acid Strength

EXPERIMENT 4 Acid Strength EXPERIMENT 4 Acid Strength Introduction Many common substances are either acids or bases. Some acids, like stomach acid are necessary for our health, while others, like sulfuric acid are dangerous and

More information

To see how this data can be used, follow the titration of hydrofluoric acid against sodium hydroxide below. HF (aq) + NaOH (aq) H2O (l) + NaF (aq)

To see how this data can be used, follow the titration of hydrofluoric acid against sodium hydroxide below. HF (aq) + NaOH (aq) H2O (l) + NaF (aq) Weak Acid Titration v120413 You are encouraged to carefully read the following sections in Tro (2 nd ed.) to prepare for this experiment: Sec 4.8, pp 158-159 (Acid/Base Titrations), Sec 16.4, pp 729-43

More information

Sugar or Salt? Ionic and Covalent Bonds

Sugar or Salt? Ionic and Covalent Bonds Lab 11 Sugar or Salt? Ionic and Covalent Bonds TN Standard 2.1: The student will investigate chemical bonding. Have you ever accidentally used salt instead of sugar? D rinking tea that has been sweetened

More information