GENIE. GENIE Genetics Education Networking for Innovation and Excellence. Wide Impact. Subject-specific and generic skills and approaches

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1 Genetics Education Networking for Innovation and Excellence The CETL Experience Challenges & Opportunities Annette Cashmore Aims Enhance learning experience - building on excellence and synergy between research, and teaching Promote change - reward and recognition of achievements in learning and teaching Embed change and share od practice University, Regionally, Nationally, Internationally Wide Impact Subject-specific and generic skills and approaches State of the Art Teaching Facilities National Network Project Work Pedagic Research Input into Strategic Policy Making 1

2 State of the Art Teaching Facilities National Network Project Work Pedagic Research Input into Strategic Policy Making National Network Regular meetings, workshops and conferences - sharing of best practice State of the Art Teaching Facilities National Network Project Work Pedagic Research Input into Strategic Policy Making Learning Futures - in the laboratory, classroom and bedroom! 2

3 Examples of Projects Outreach Examples of Projects Games for teaching meiosis DNA model building tutorial Resources for teaching DNA sequencing Collaboration with University of Kent Chromosomes & Education Production of videos Animations 3

4 Examples of Projects Modules Genetics Research in Medicine Social Ethical & Legal Implications of Genetics (Blogs, Wikis and Podcasts) Role play exercises for genetics & ethics Genetics & Ethics multifaith implications Bioethics Bytes - (Blog and Podcasts) Examples of Projects NHS Genetic link worker GPs and local communities Challenge of one year masters programmes Problem based learning group-work vs. on-line exercises 4

5 State of the Art Teaching Facilities National Network Project Work Pedagic Research Input into Strategic Policy Making Welcome to the Virtual Genetics Education Centre Genetics information & education resources for teachers and learners in HE, schools, health professionals, and the general public. Enter the VGEC Introduction to Genetics Higher Education Health & Disease Schools & Colleges Games & Quizzes Genetics & Society Genetics in the News Introduction to Genetics Higher Education Health & Disease Schools & Colleges Games & Quizzes Genetics & Society Genetics in the News Chromosomes, Mitosis and Meiosis Patterns of Inheritance DNA Structure and Chromosome Organisation Recombinant DNA and Genetic Tools Gene Action Learning Resources Development of new resources Selection and assessment of existing resources from other sources Blending elearning with more traditional approaches Introduction to Genetics Higher Education Health & Disease Schools & Colleges Games & Quizzes Genetics & Society Genetics in the News Learning Resources Groupwork Problem-based learning Topic material Laboratory practicals Simulated practicals Genes and Cancer Animations Developmental Genetics Video content Learning Resources Population Genetics Miscellaneous 5

6 Introduction to Genetics Higher Education Health & Disease Schools & Colleges Games & Quizzes Genetics & Society Genetics in the News Learning Resources Laboratory Classroom Bedroom!! Groupwork Problem-based learning Topic material Laboratory practicals Simulated practicals Animations Video content Miscellaneous Production of Medically Important Proteins Using Recombinant DNA Techniques Start FLOW DIAGRAM OF CLONING A GENE You must now decide how to clone your gene : Vectors and DNA fragments 8 Student-centred problem-based group exercises: small group teaching on a large scale! Production of Medically Important Proteins Using Recombinant DNA Techniques Construct recombinant DNA molecules Each carries a different fragment Introduce into E. coli Plate out - each colony will contain only one type of recombinant molecule (1) cdna library Go to 9 Start Back (2) (2) genomic library (3) PCR amplification Go to 10 Go to 11 6

7 Polymerase Chain Reaction (PCR) A procedure to amplify a fragment of DNA. YEAST VECTORS YEp213 Multicopy, DNA plasmid Shuttle vector - bacterial and yeast origins of replication (ORI) 2µm sequences (ORI andstb) Selective markers : Yeast : auxotrophic marker - LEU2 gene (LEU2) Bacteria : Ampicillin resistance (Amp R) Tetracycline resistance (Tet R) EcoRI Cloning vector LEU2 ORI PstI 36 Plasmid Extrachromosomal DNA molecules found naturally in bacterial cells. Modified plasmids are used as cloning vectors or vehicles. PstI EcoRI YEp213 STB EcoRI Back More information Animation of PCR reaction Back Go to 37 PstI Amp R Tet R BamHI Back More information FAILURE!!!!! You have not been able to express your gene in this host - vector system. The reasons for this are : You have cloned into pbr322 or YEp213. These are cloning vectors which have no expression signals, also the insert DNA does not have any expression signals therefore there is no expression of the protein. Choose an expression vector Go to N.B. Another possible reason for the absence of expression is that the gene may have been inserted into the vector in the incorrect orientation, therefore is unable to use the vectors promoter sequences. If the sequence or restriction map of the gene is known then restriction mapping can be used to determine whether this is the case. SUCCESS 83 Well done!!!! You have successfully produced your protein and it is biologically active. Producing a cell that synthesises large amounts of a desired protein is only the first stage in achieving a useful process. It is important to be able to recover the protein by a simple, economical method that results in high yields of a biologically active protein. Cell cultures can be scaled up and grown in large fermentors, and then the protein can be purified using a number of methods. Evaluation electronic Cohort split group session 7

8 Pilot Formative assessment Challenges 14 Electronic 13 Group sessions MCQ 48, 68, 65% Design 42, 57.5% MCQ 49, 59, 58% Design 38, 62% Multiple choice - test knowledge Outline of experimental design - test analytical skills - ability to integrate and coordinate knowledge Are people using the VGEC? Do they find it useful? Is it easily accessible? Evaluation of linked sites Evaluation of existing on-line resources Evaluation of new resources Opportunities Questionnaire feedback Engaging members of our network in research projects Engaging other users in research projects Evaluation made available Agreement to share resources Development of novel resources Optimising combination of face-to-face and elearning Chris Talbot, Jon Scott, Chris Wilmott Core Team Mark Goodwin, Cas Kramer Teaching Fellows Nicola Suter-Georgini Ruth Barber, Jo Badge Research Associates Zoe Rosser, David Hawkridge Ila Patel, Angela Thomas Administrative Support Stephen Maw (HEA Biosciences Subject Centre) Annette Cashmore Director 8