CTY Genetics Syllabus

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1 CTY Genetics Syllabus

2 Week 1: Review and Mendelian Genetics What (DUE DATE) 1 Introduction and Review Morning Classroom Policies/ Ice Breaker Game/Introductions Syllabus Distribute Syllabus, Discuss Course Outline Lab Safety Lab Safety Introduction and equipment practice Practice of Science Introduction to Pipettes Cell Biology Scientific Method and Lab Reports Mitosis/Meiosis Safety Rules Posters- Model Organisms Afternoon Lecture/Notes to review Cell Biology Scientific Drawing Practice Draw/Label Animal Cells Lecture/Notes- Review Mitosis and Meiosis Build and Compare Models of Mitosis/Meiosis Evening (Assignments) Review Cell Biology/Mitosis/Meiosis Reading on Patterns of Inheritance Lab Notebook Biographies- on page 1 of the lab notebook, write a short biography of yourself and your study of science. Write a short paper in your lab notebook about the perfect model organism for studying genetics (~1 page) 2 Mendelian Genetics Macromolecule Properties and Identification Mendelian Genetics and Punnett Squares Morning Model Organism Characteristics Lecture/Notes on Macromolecules Carbs/Protein/Nucleic Acid/Lipid Lab Safety and Procedures Review ID of Macromolecules Lab Afternoon Lecture/Class notes -Mendel, dominant/recessive/, genotype/phenotype, mono/dihybrid crosses, Laws of Independent Assortment and Random Segregation Practice with Punnett Squares Lecture/Class notes-pedigrees Using Pedigrees to Track Inbreeding and Disease Alleles Activity Evening Introduction to Geniverse (online genetics web activity) Course Outline CTY Genetics Page 2

3 What 3 Mendelian Morning Genetics pt 2 Warm-Up challenge- Murder Mystery Codominance Lecture/Notes on Mendelian Genetics part 2 Multiple alleles Codominance, multiple allele traits, Incomplete incomplete dominance Dominance Genetics in Harry Potter s World Activity Pleiotropy Lecture/Notes on Pleiotropy and Epistasis Epistasis Discussion and Practice using Pedigrees with Complex Traits Afternoon Discussion- Genetic Counseling Case Study/Debate Not Exactly parts 1&2 Evening Independent Student Research on a Genetic Disease and poster preparation to share in class tomorrow Reading on Complex Traits 4 Genetic Diseases Penetrance Expressivity 5 Chromosomal Basis of Genetics Sex Linkage Chromosome Structure Morning Student Presentations on Genetic Diseases The Making of the Fittest - Afternoon HHMI Mendelian Genetics, Probability, Pedigree Activity/Worksheets on Sickle Cell Trait Penetrance and Expressivity of Traits Survey of Student Phenotypes/Genotypes Ratios Evening Independent Reading on Chromosome Structure pgs 4-9; ; Guided Review sheet for Classical Genetics Quiz on Day 5 Morning Quiz on Mendelian Genetics Chromosomal Basis of Genetics Discovery of Sex-Linkage Advantages/Disadvantages of Sex-Linkage Afternoon Introduction to Chromosome Structure Building Model Chromosomes Chromosomal Abnormalities Paper Karyotypes for Genetic Disorders Modern Techniques for Analyzing Chromosome Structure and Number Sunday Evening Finish Geniverse Simulation Course Outline CTY Genetics Page 3

4 Week 2: Molecular Genetics and The Central Dogma 6 Gene Linkage Discovery of Linkage and Recombination χ 2 Tests for linkage Morning (140 Warm-Up Discussion- Hardest and Easiest Challenges in Geniverse and WHY (15 Lecture/Class Notes- Thomas Hunt Morgan, the fruit fly, discovery of linkage (45 Guided introduction- χ 2 Tests (45 Paired Practice using χ 2 tests (30 Afternoon (120 Mapping Genes with Linkage Markers Activity (100 Lecture/Review of Gene Linkage and Mapping (20 7 DNA Structure Discovery of DNA Structure DNA Building Blocks Evening (120 Gene Mapping Now and Then (120 Minutes) Web-reading and online quizlet Morning (140 Warm-Up Discussion and Cases: Research Ethics (20 Lecture/Class Notes- Discovery of DNA (20 Class Debate: The role of Rosalind Franklin (15 Lecture/Class Notes Structure of DNA (40 Model Building- Structure of DNA -paired construction and presentation to the class (45 Review of Lab Notebook and Lab Report Format (10 Afternoon (120 Lab Activity- Isolation of DNA and comparison of diploid and polyploid DNA yields (120 Evening (120 Individual Lab Report Preparation Pop-Culture Genetics Readings Course Outline CTY Genetics Page 4

5 8 Mutation DNA Replication Causes and Results of Mutation Epigenetics Imprinting and Disease 9 DNA-RNA-Protein Transcription Translation Bacterial and Viral MolBio Tools Morning (140 Warm-Up Discussion: Molecular Biology in Pop Culture (15 Lecture/Class Notes: DNA Replication (45 Activity: Human PCR Machines (30 Lecture/Class Notes: Mutations in DNA (45 Afternoon (120 Lecture/Class Notes- Epigenetics, Imprinting and Disease (60 Class Discussion- Nuclear Reprogramming (20 Research/Reading Activity Who/What is Mitochondrial Eve? (40 Evening (120 Case Study Discussion/Debate Not Exactly parts 3&4 (120 Continuation of genetic counseling case study with molecular data for analysis Morning (140 Warm-Up Activity: Draw and Label a Gene (10 Lecture/Class Notes: Transcription and Translation (25 Scientific Drawing- Production of a Protein from DNA to export (20 Class Discussion- Review of Mutation Effects on DNA (10 Modeling Activity- DNA-RNA-Protein using paper/beads/string to transcribe/translate/fold gene products(40 Begin Plasmid Mapping Lab Activity (35 Restriction Digest Afternoon (120 Plasmid Mapping Gel Electrophoresis (45 Lecture/Class Notes- Bacterial and Viral DNA Tools (30 pglo Transformation Lab Part 1 (45 Evening (120 Plasmid Mapping Data Analysis/plasmid assembly (50 Student guided study for Quiz #2- central dogma Course Outline CTY Genetics Page 5

6 10 Regulating Gene Expression The lac operon sirna/rnai microarrays qpcr Morning (140 Quick Questions: Review Plasmid Mapping (15 Quiz #2: Central Dogma (20 Lecture/Class Notes: Methods of gene regulation- promoters, silencers, and operons (35 HexBot Battles of the lac operon (30 Robot simulation of effect of inducers/repressors Scientific Drawing: Tracing genes from DNA-RNA-Protein Structure with ID of regulator locations in each step (20 Lecture/Class Notes- Assays for gene expression- microarray and qpcr (20 Afternoon (120 sirna Video- Nature Video (10 Nature Video Link to Youtube Discussion of Knockdown vs knockout regulation of gene expression- why bother? (15 HexBot Battles part 2: design a model of sirna (30 pglo Transformation Lab- part 2 (60 Evening (120 (Sunday?) Independent student work on pglo lab reports (60 Visualizing Gene Expression Patterns- Click and Learn activity from HHMI Biointeractives (45 ( Course Outline CTY Genetics Page 6

7 Week 3: Topics in Genetics: Population Biology, Ecology, Biotechnology 11 Population Genetics Variation Selection Speciation Darwin Hardy-Weinberg Evolution Morning (140 Warm-Up Activity- Guided Primary Literature Reading- Science 11 February 1994: Vol. 263 no pp (M. Chalfie et al) (30 GFP as a living cell label Lecture/Class Notes- Variation, Selection, Speciation, Darwin and Hardy/Weinberg (45 Student Worksheet on Hardy-Weinberg (15 Student DNA Sample Isolation for Hardy-Weinberg Lab (50 Afternoon (120 Hardy-Weinberg Lab- gel electrophoresis (45 Got Lactase? HHMI short video and discussion (30 HHMI web activity on Lactase regulation (15 Hardy-Weinberg Lab Data Analysis/Summation (30 Evening (120 HHMI- Using DNA to Trace Human Migration (35 DNA Homology as Evidence for Evolution Hardy-Weinberg Lab Report (60 Textbook reading about recombinant DNA strategies (25 12 Recombinant DNA, Transgenics, Cloning/Stem Cells Totipotent/ pluripotent/es stem cells ips Cells Nuclear Reprogramming Cell Fate and Patterning Morning (140 Warm-Up Discussion- Is it a clone? (10 Lecture/Class Notes- Transgenic Techniques (30 HHMI Transgenic FlyLab computer simulation (60 Class Debate- Ethics of Cloning (40 Afternoon (120 Lecture/Class Notes: Introduction to Stem Cells (30 Primary Literature Reading/Presentation in teams: ips and nuclear reprogramming (Yamanaka and Gurdon papers) (60 Class Discussion- Future of Stem Cell Research (30 Course Outline CTY Genetics Page 7

8 Evening (120 Hardy-Weinberg Lab Notebook Reports Student selected independent reading and written summary of a current published work in ips/cloning/stem cell research (75 13 Cancer as a Genetic Disease Cell Cycle Oncogenes DNA Fingerprinting Genetic-Based Treatment of Disease Morning (140 Student ips research presentations (60 Lecture/Class Notes- Cell Cycle, p53/ Rb/oncogenes (45 I always wondered and This is weird, but what would happen if Genetics Q&A/Discussion (20 Lecture/Class Notes- Telomeres and Chromosomal Aging (15 Afternoon (120 Review Lab- DNA Crime Solving (120 Evening (120 DNA Fingerprinting Stories Review for Post-Assessment (90 14 Genetic Technology and You GMOs Morning (140 Quick Review and Q&A for Post-Assessment (15 Post Assessment (45 Class Discussion- Who Owns your Genes? (20 Lecture/Class Notes- GMO and GMO Food (40 Class Debate. Yes or No to GMOs? (20 Afternoon (120 GMO Food Testing Lab and discussion (120 Evening (120 Gattaca- film and discussion Course Outline CTY Genetics Page 8

9 15 Ethical Use of Genetic Technology Ethics Genetic/Genomic Based Treatment and Diagnosis Morning (140 Lecture/Class Notes- Genetic and Genomic Disease Treatment Case Study parts 5+6 (60 Student Discussion/Debate on Genetic Technology and Ethics (60 Final Q&A about Genetics Topics (20 Course Outline CTY Genetics Page 9

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