Bruker Daltonics. MALDI Biotyper. Microorganism Identification and Classification MALDI-TOF. think forward

Transcription

1 Bruker Daltonics MALDI Biotyper Microorganism Identification and Classification think forward MALDI-TOF

2 Identify Microorganisms in Minutes Searching for the culprit? Fast and reliable strain-specific identification of microorganisms, like bacteria, yeasts, or fungi, is mandatory in clinical routine monitoring, food processing quality control or environmental and taxonomical research. Benefit from ultra-fast analysis time and a simple consistent analysis workflow with high differentiation power. The molecular fingerprint Discover bacteria s identity by its own unique molecular composition. In a MALDI-TOF mass spectrometer, proteins and peptides are arranged in a spectrum with increasing mass revealing a characteristic peak pattern, even for reliable differentiation of strains: The individual fingerprint. Sample amount is not critical The sample material can be a single colony from overnight culture or a centrifuged portion of a liquid culture. Biological material is prepared as smear or extract respectively, representing millions of cells roughly, although a minimum of 10 5 cells can be measured accurately on a standard MALDI sample target plate.

3 The Workflow: Only Little Interaction Required 1. Open MALDI Biotyper control wizard Ps. mendocina DSM Define your project Ps. oleovorans B396 Ps. fluoreszens B340 Ps. veronii DSM11331 Ps. putida DSM291 Ps. putida B Allocate your samples It takes only minutes to receive results on the screen. You are only five mouse clicks away from your results. 4. Initiate measurement 5. Receive results

4 Full Bioinformatics Capabilities Identification by peak-matching Sub-Typing In depth analysis of acquired data sets is possible by applying different bioinformatic tools like weighted pattern matching algorithms, correlation and principal component analysis. Principal component analysis Principal component analysis, for example, is an excellent tool for fine analysis of closely related isolates, species or subspecies. Dendrogram generation Dendrograms are created literally within seconds from all main spectra selected in one step. Creation of own reference files Add your own references or create a complete new reference database by yourself straightforwardly with a few additional mouse clicks. Typical MALDI Biotyper 2.0 result window after off-line processing of an unknown sample. Left: Peak matching window shows the measured spectrum compared to the reference spectrum. Right window shows the hits in the database. Multivariate analysis of pseudomonas species 3D scatter plot of the first three principle component scores for six individual species of the genus Pseudomonas, Pseudomonas veronii, P. fluorescens, P. mendocina, P. oleovorans, P. putida strain B401, P. putida strain DSM291 Dendrogram generation Clostridia dendrogram created with MALDI Biotyper; finest differences between strains can readily be evaluated. Data were generated in cooperation with Prof. Krüger and Dr. Grosse-Herrenthey, Institute of Bacteriology and Mycology, University of Leipzig, Germany.

5 In One Simple Step Ease of operation: Real-Time analysis Ps. mendocina DSM50017 Ps. oleovorans B396 Ps. fluoreszens B340 Ps. veronii DSM11331 Ps. putida DSM291 Ps. putida B401 Single bacterial colonies or other biological material can be directly applied to a MALDI sample target and introduced to a FLEX series MALDI-TOF mass spectrometer. Spectra acquisition is completed after a few minutes and data evaluation is directly linked to the measurement. This simple and exclusive workflow is adequate for most microorganisms, avoiding the need to perform gramstaining, oxidase- or any other tests. Sophisticated instrumentation With robust and reliable Bruker Daltonics FLEX series instrumentation, fast measurements with high reproducibility are possible. The high sensitivity of the instruments allows identification of minimal sample amounts. When employing the renowned Bruker AnchorChip TM technology, scientists need even less biological material. Performance on the bench: the microflex TM The molecular fingerprint is used for pattern matching. Sophisticated recalibration and statistical algorithms allow robust and accurate identification. Matching scores supported by color codes are used for ranking of results. Ready-to-Go database MALDI Biotyper integrates a ready-touse reference library comprising thousands of individual strains and is growing continuously. The system is open and can be easily expanded by specific requests of the user including data for ID of bacteria, yeasts and fungi.

6 Bruker Daltonics is continually improving its products and reserves the right to change specifications without notice. BDAL , LS-30/3 Technical Specifications Fields of application Clinical routine monitoring, environmental and taxonomical research, food processing quality control Features identification, taxonomical classification, or dereplication of microorganisms Sample preparation / handling Very simple and rapid preparation. Very sensitive, amounts of roughly 10 5 cells are required No initial assessment, each sample handled in the same way: No gram staining, oxidase- or any other tests and conditions choice Samples Bacteria (gram+ / gram-), yeasts, fungi, spores Instrumentation Fast measurements with high reproducibility on a microflex TM, autoflex TM or ultraflex TM MALDI-TOF mass spectrometer Software MALDI Biotyper, COMPASS TM for FLEX series incl. flexanalysis TM Speed of analysis Minutes from colony to result for a single sample, approx. 1.5 hours from colony to result for 96 samples For research use only. Not for use in diagnostic purposes. Prof. Dr. Erko Stackebrandt, Managing Director of DSMZ There was an urgent need for a next generation technique for the fast, convenient and cost-effective identification of microbial strains. Bruker Daltonics MALDI Biotyper is well suited for this purpose as it fulfills these requirements and as it has proven to be very robust and reproducible. Bruker Daltonik GmbH Bremen Germany Phone +49 (421) Fax +49 (421) sales@bdal.de Bruker Daltonics Inc. Billerica, MA USA Phone +1 (978) Fax +1 (978) ms-sales@bdal.com