Application Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method

Size: px
Start display at page:

Download "Application Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method"

Transcription

1 Application Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method Introduction During the last decade, the complexity of samples addressed in bottom-up proteomics studies has increased along with the instrument s capabilities to generate more and more detailed information from these complex samples. Instrument speed, sensitivity and mass accuracy have reached new levels, enabling the generation of more data from increasingly complex samples and to better cope with the advances in U-HPLC separation. Whilst slower than other architectures - like ion traps - at the beginning of the century, Q-TOF and UHRQ-TOFs have now reached new levels in both speed and sensitivity. These improvements allow Q-TOF systems to generate identification and quantification results which are fully comparable with the ones obtained from the latest bench-top FT-based instruments, while keeping the advantage of preserved resolution at high acquisition speeds. Whatever technology is used, the number of unproductive spectra is relatively high, typically in the range of 60-70% of all spectra for a human cell line digest. The impossibility to link these spectra to a known protein most often comes from the occurrence of unexpected modifications, inaccurate database information or from spectra of poor quality. The two first reasons can be addressed by adjusting Authors Dr. Stephanie Kaspar, Dr. Markus Lubeck, Dr. Alexander Harder Bruker Daltonik GmbH, Bremen, Germany Pierre-Olivier Schmit Bruker Daltonique S.A, France Keywords compact impact Proteomics Biomarker Discovery Instrumentation and Software compact impact DataAnalysis ProteinScape ProfileAnalysis

2 the search method or performing de-novo sequencing, however trying to minimize the number of garbage spectra was always a matter of compromise between the acquisition speed and the number of MS points in the chromatogram (defining the Qual/Quant capabilities of a system). Moreover, if one compromise was valid for a sample with a given dynamic range and average concentration it wasn t the best option if these conditions were changed. With the compact Benchtop ESI-QTOF, we introduce a new release of the Compass acquisition software. It enables a series of new acquisition strategies that make it possible to use a single acquisition method to deliver optimal results, whatever the sample s initial concentration or dynamic range. The instrument s improved robust hardware enables this new benchtop system to deliver state-of the art results in bottom-up proteomics. This improved hardwaretogether with the new software features will also be available on the UHR-Q-TOF impact. Experimental The human HeLa cell line was digested using trypsin ng lysate were injected into a nano LC system equipped with a Dionex Pepmap C18 25cm x 75µm column (2µm particles) coupled to a compact Benchtop Q-TOF or an impact benchtop UHR-QTOF both running with the new Compass 1.6 Acquisition and processing software. Both systems were operated with an automated internal re-calibration, and once acquired the data was processed with Bruker s ProteinScape bioinformatics software suite. Database searches were performed with Mascot 2.4 triggered by ProteinScape. The accepted peptide False Discovery Rate was set to 1%. Results and Discussion Compass 1.6: improved auto MS/MS acquisition strategies In the past, tuning the ideal auto MS/MS method has always been a matter of compromise: Compromise between identification results and quantification capabilities: only 3 to 4 MS per chromatographic peak are required to sample efficiently the precursor peaks population, but at least 6-7 are required to properly define the peak and make it possible to use it for accurate quantitation. The number of points were not predictable as directly defined by the number of precursors chosen between each MS/MS (20 for instance for a top 20 method) and by the time requested to perform each MS/MS. Compromise between the spectral quality and the acquisition speed: whatever active exclusion strategy used, the choice was either to favor the spectra quality or the total number of precursors from different species. The same was true for the acquisition s speed choice. A fast MS/MS acquisition speed favors the fragmentation of a higher number of species but degrades the spectral quality obtained from lower intensity precursors. The IDAS (Intensity Dependent Acquisition Speed) and RT 2 (RealTime Re-Think) functionalities of Compass 1.6 now make these compromises unnecessary. They consist of the following: with IDAS, the cycle time is fixed instead of the number of precursors selected for MS/MS, and the acquisition speed is adapted depending on the precursor intensity (Fig 1). This way, the number of precursors is directly adapted to the available precursor intensity, avoiding wasting time with unnecessary MS survey scans between many intense precursors and preventing missing low-intensity compounds if the acquisition is slowed down for very low abundant samples. Moreover, as the number of MS points defining a chromatographic peak is stable, the Qual/Quant capabilities of the instrument are maintained over the entire chromatographic run. Finally, the only parameter requiring adaptation is the time spacing of MS survey scans, which depends directly of the chromatographic performances delivered by the LC system used with the instrument. However, the IDAS does not handle the issue of inappropriate exclusion strategies, which can result in the same peptide being selected for fragmentation a number of times where its intensity has not improved between the different points, and consequently losing the opportunity to fragment a peptide belonging to another species. This situation is addressed by the RT 2 (RealTime Re-Think) functionality. RT 2 is a dynamic adaptation of the exclusion strategy (Fig 2): once a precursor is fragmented it is excluded for the typical time of a chromatographic peak unless its intensity increases by more than a defined factor (typically set to 5). This ensures, that a precursor is only re-fragmented if a substantial gain in spectral quality is to be expected from a second analysis. Other algorithms, which try to anticipate the peak s maximum and trigger an MS/MS selectively at that time, require well described peaks of reasonable intensity and tend to completely miss low abundant precursor ions appearing only in a low number of MS scans. RT 2 is the most robust approach here.

3 Intensity Dependant Acquisition Strategy Auto cycle time [s] MS MS MS MS Auto cycles 20Hz Hz spectra rate Figure 1: IDAS: Illustration showing an example of time cycle handling with the Independent Data Acquisition Strategy: the number of precursors is chosen dynamically to maintain the cycle time constant and the MS/MS spectra rate is adapted to each precursor intensity. RT 2 : RealTime Re-Think A Figure 2: RT 2 : RealTime Re-Thinks management of the Dynamic exclusion. The basic exclusion time is fixed to slightly longer than the base width of the chromatographic peak. Case A: the MS/MS is triggered near the peak maximum the precursor is not fragmented again as intensity is not increasing by the factor set for RT2. Case B: the MS/MS is triggered a first time near the peak base theprecursor is reconsidered for fragmentation only if its intensity has increased (typically by 3-5 fold). B Global proteomics ID results obtained with the compact from a complex protein digest The new compact system powered by Compass 1.6 has been evaluated on a HeLa human cell line digest. A 20 ng injection of the sample separated and analyzed with IDAS and RT 2 activated for a 2-20Hz dynamic method resulted in the identification of 631 proteins at a 1% peptide FDR. The same method used with 3µg of the same sample and a 4h gradient resulted in 2575 ID s (Fig 3). This result is comparable to what could be achieved with a 2µg injection and a 3 hours gradient on an impact system operating with a comparable acquisition method but without IDAS and RT 2. The compact s typical resolution is 1222 m/z whereas the impact s is at the same mass. That means that the improvement in the parent selection combined with the improved spectral quality have partially compensated for the loss of resolution and almost driven the compact s ID potential to the formers impact level. Moreover, the gain in spectral quality obtained thanks to the IDAS and RT 2 combination makes it possible to drive the MS/MS spectrum ID rate from a 35% level to a 50% level. This translates into an on average higher quality of spectra obtainable, as illustrated in (Fig 4). Thus these new features further increase the already high spectral quality delivered by the instrument (Fig 5). The same level of gains could be obtained from the impact when used with the new acquisition strategy.

4 compact: ID s from complex mixtures m/z 1400 Instrument compact with new SW 1200 Sample (Hela) 3µg 20ng Gradient 4h 90min % FDR Time [min] Figure 3: compact ID s from complex mixtures. The survey view is issued from the separation and acquisition of 3µg of a HeLa cell lysate tryptic digest on a 50cm column with a 4h gradient. The resulting number of ID s was 1% FDR. compact: spectral 20 Hz Figure 4: Focus on the Compact spectral 20Hz MS/MS acquisition rate. Mascot view of two deconvoluted ions 20Hz from a 3 µg injection of a HeLa cell lysate separated on a 240min gradient. compact spectral quality: broad mass transfer Intens MS2( ), eV, min # Pro m/z m/z Figure 5: Display of the intrinsic spectral quality of the compact (further enhanced by IDAS and RT 2 ): this spectrum particularly illustrates the instrument s broad mass range transmission, which is ideal for qual/quant with isobaric tags (itraq/tmt).

5 Universal method : illustration with the impact The results obtained for various amounts of the HeLa human cell digest on an impact using a 90min gradient are displayed in (Fig 6). A common method was used for these samples, meaning that the dynamic range created by the distribution of the injected amounts adds to the sample s own dynamic range. The benefit of IDAS and RT 2 combination is that the system automatically adapts the acquisition rate to the amount of sample available, preserving both the spectral quality for optimal ID rates and the chromatographic resolution for qual/quant purposes. The preserved spectral quality is well illustrated by the relatively small drop in the average Mascot ions score between different sample amounts injected. The overall increase in performance is evident at all levels where the 2087 and 2097 ID s obtained for the two distinct 1µg injections represent roughly a 10% improvement in comparison with the same amount injected on the system without IDAS and RT 2. For this amount injected the percentage of significant hits increases from a 35% to a 54 % level. The 35% significant hits level is obtained with an injected amount of 100ng, that is for a 10-fold decrease injected amount. Conclusion The compact Q-TOF and impact UHR Q-TOF s state of the art capabilities for protein ID s, issued from their combination of robustness, speed, sensitivity, resolution and mass accuracy, are further improved by the use of the new IDAS and RT 2 protocols available in Compass 1.6. In addition to improving the method s usability for samples showing different complexities, this approach ensures a better global spectral quality, translated in higher Mascot ion scores and larger proportion of identified spectra. This versatility is enhanced as the method makes it possible to preserve optimal chromatographic profiles for every analysis, therefore enabling accurate quantitation. The new Compass 1.6 features make it possible to increase the global proteomics usability and capabilities of both compact and impact systems. The full scope of these capabilities will be exploited through the ProteinScape Bioinformatic suite that enable the extraction of useful biological information from the high quality data generated. These first results underline a very positive influence of the new precursor selection management. However, there is still room for improvement in managing the different charge states of a same compound present under a chromatographic peak. Use of universal method with impact Figure 6: Use of Universal method with the impact. Results issued from the analysis of 10 to 1000 ng of a HeLa cell lysate tryptic digest with a 90min gradient. The number of ID 1% FDR are obtained with percolator, the average mascot ion scores are displayed without percolator.

6 Bruker Daltonics is continually improving its products and reserves the right to change specifications without notice. Bruker Daltonics , LCMS-81, Further information [1] Bruker App-Note LCMS-67 3,500 Proteins Identified from a Human Cell Lysate Using Complementary MALDI and ESI Data [2] Bruker Tech-Note TN-27 ProteomeQuant A Highly efficient Solution for Biomarker Discovery and label-free Identification of Regulated Proteins in Biological Systems [3] Bruker Tech-Note TN-43 Targeted Proteomics Using HR-XIC Filtering with Skyline For research use only. Not for use in diagnostic procedures. Bruker Daltonik GmbH Bremen Germany Phone +49 (0) Fax +49 (0) Bruker Daltonics Inc. Billerica, MA USA Phone +1 (978) Fax +1 (978) Fremont, CA USA Phone +1 (510) Fax +1 (510)

ProteinScape. Innovation with Integrity. Proteomics Data Analysis & Management. Mass Spectrometry

ProteinScape. Innovation with Integrity. Proteomics Data Analysis & Management. Mass Spectrometry ProteinScape Proteomics Data Analysis & Management Innovation with Integrity Mass Spectrometry ProteinScape a Virtual Environment for Successful Proteomics To overcome the growing complexity of proteomics

More information

In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates

In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates Using the Explore Workflow on the AB SCIEX TripleTOF 5600 System A major challenge in proteomics

More information

Effects of Intelligent Data Acquisition and Fast Laser Speed on Analysis of Complex Protein Digests

Effects of Intelligent Data Acquisition and Fast Laser Speed on Analysis of Complex Protein Digests Effects of Intelligent Data Acquisition and Fast Laser Speed on Analysis of Complex Protein Digests AB SCIEX TOF/TOF 5800 System with DynamicExit Algorithm and ProteinPilot Software for Robust Protein

More information

Bruker ToxScreener TM. Innovation with Integrity. A Comprehensive Screening Solution for Forensic Toxicology UHR-TOF MS

Bruker ToxScreener TM. Innovation with Integrity. A Comprehensive Screening Solution for Forensic Toxicology UHR-TOF MS Bruker ToxScreener TM A Comprehensive Screening Solution for Forensic Toxicology Innovation with Integrity UHR-TOF MS ToxScreener - Get the Complete Picture Forensic laboratories are frequently required

More information

Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software

Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software Abstract Presented here is a case study of a walk-up liquid chromatography

More information

AB SCIEX TOF/TOF 4800 PLUS SYSTEM. Cost effective flexibility for your core needs

AB SCIEX TOF/TOF 4800 PLUS SYSTEM. Cost effective flexibility for your core needs AB SCIEX TOF/TOF 4800 PLUS SYSTEM Cost effective flexibility for your core needs AB SCIEX TOF/TOF 4800 PLUS SYSTEM It s just what you expect from the industry leader. The AB SCIEX 4800 Plus MALDI TOF/TOF

More information

The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring

The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring Delivering up to 2500 MRM Transitions per LC Run Christie Hunter 1, Brigitte Simons 2 1 AB SCIEX,

More information

amazon SL Innovation with Integrity Setting New Standards in Performance, Simplicity and Value Ion Trap MS

amazon SL Innovation with Integrity Setting New Standards in Performance, Simplicity and Value Ion Trap MS amazon SL Setting New Standards in Performance, Simplicity and Value Innovation with Integrity Ion Trap Best-In-Class Ion Trap Mass Spectrometer for Routine Analysis The amazon SL entry-level system is

More information

Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays

Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays Scheduled MRM HR Workflow on the TripleTOF Systems Jenny Albanese, Christie Hunter AB SCIEX, USA Targeted quantitative

More information

Application Note # LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities

Application Note # LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities Application Note # LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities Introduction Glycosylation is one of the most common and

More information

# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water

# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water Application Notes # LCMS-35 esquire series Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water An LC-APCI-MS/MS method using an ion trap system

More information

Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests

Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Application Note: 386 Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Rosa Viner, Terry Zhang, Scott Peterman, and Vlad Zabrouskov, Thermo Fisher Scientific, San Jose, CA,

More information

Simultaneous qualitative and quantitative analysis using the Agilent 6540 Accurate-Mass Q-TOF

Simultaneous qualitative and quantitative analysis using the Agilent 6540 Accurate-Mass Q-TOF Simultaneous qualitative and quantitative analysis using the Agilent 654 Accurate-Mass Q-TOF Technical Overview Authors Pat Perkins Anabel Fandino Lester Taylor Agilent Technologies, Inc. Santa Clara,

More information

Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow

Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow Abstract There is high demand in clinical research and forensic toxicology for comprehensive,

More information

MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification

MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification Gold Standard for Quantitative Data Processing Because of the sensitivity, selectivity, speed and throughput at which MRM assays can

More information

泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics

泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics 泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics 2014 Training Course Wei-Hung Chang ( 張 瑋 宏 ) ABRC, Academia

More information

Aiping Lu. Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn

Aiping Lu. Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn Aiping Lu Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn Proteome and Proteomics PROTEin complement expressed by genome Marc Wilkins Electrophoresis. 1995. 16(7):1090-4. proteomics

More information

impact II Innovation with Integrity Get the full picture the first time UHR-TOF MS

impact II Innovation with Integrity Get the full picture the first time UHR-TOF MS impact II Get the full picture the first time Innovation with Integrity UHR-TOF MS Sensational Capabilities of impact II Optimize your LC-MS methods without compromising performance: impact II delivers

More information

Experimental workflow

Experimental workflow Experimental workflow Pg. 3 Lysis Protein quant Protein precipitation Pg. 4 Digest Pgs. 5&6 Label Peptides Peptide quant Ratio check Search, filter & compile data Pgs. 9-12 Combine Samples LC-MS3 Pg. 8

More information

ProteinPilot Report for ProteinPilot Software

ProteinPilot Report for ProteinPilot Software ProteinPilot Report for ProteinPilot Software Detailed Analysis of Protein Identification / Quantitation Results Automatically Sean L Seymour, Christie Hunter SCIEX, USA Pow erful mass spectrometers like

More information

Choices, choices, choices... Which sequence database? Which modifications? What mass tolerance?

Choices, choices, choices... Which sequence database? Which modifications? What mass tolerance? Optimization 1 Choices, choices, choices... Which sequence database? Which modifications? What mass tolerance? Where to begin? 2 Sequence Databases Swiss-prot MSDB, NCBI nr dbest Species specific ORFS

More information

Session 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists

Session 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists Program Overview Session 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists Session 2. Principles of Mass Spectrometry Session 3. Mass spectrometry based proteomics

More information

Error Tolerant Searching of Uninterpreted MS/MS Data

Error Tolerant Searching of Uninterpreted MS/MS Data Error Tolerant Searching of Uninterpreted MS/MS Data 1 In any search of a large LC-MS/MS dataset 2 There are always a number of spectra which get poor scores, or even no match at all. 3 Sometimes, this

More information

Global and Discovery Proteomics Lecture Agenda

Global and Discovery Proteomics Lecture Agenda Global and Discovery Proteomics Christine A. Jelinek, Ph.D. Johns Hopkins University School of Medicine Department of Pharmacology and Molecular Sciences Middle Atlantic Mass Spectrometry Laboratory Global

More information

Tutorial for Proteomics Data Submission. Katalin F. Medzihradszky Robert J. Chalkley UCSF

Tutorial for Proteomics Data Submission. Katalin F. Medzihradszky Robert J. Chalkley UCSF Tutorial for Proteomics Data Submission Katalin F. Medzihradszky Robert J. Chalkley UCSF Why Have Guidelines? Large-scale proteomics studies create huge amounts of data. It is impossible/impractical to

More information

Tutorial 9: SWATH data analysis in Skyline

Tutorial 9: SWATH data analysis in Skyline Tutorial 9: SWATH data analysis in Skyline In this tutorial we will learn how to perform targeted post-acquisition analysis for protein identification and quantitation using a data-independent dataset

More information

for mass spectrometry calibration tools Thermo Scientific Pierce Controls and Standards for Mass Spectrometry

for mass spectrometry calibration tools Thermo Scientific Pierce Controls and Standards for Mass Spectrometry Thermo Scientific Pierce Controls and Standards for Mass Spectrometry calibration tools for mass spectrometry Ensure confidence in instrument performance with Thermo Scientific Pierce Calibration Solutions

More information

prime Innovation with Integrity The multidimensional path to the Proteome Mass Spectrometry

prime Innovation with Integrity The multidimensional path to the Proteome Mass Spectrometry prime The multidimensional path to the Proteome Innovation with Integrity Mass Spectrometry Reach for the Full Potential of Proteomics. Open Your Eyes to PRIME The Proteome is far more complex than was

More information

Proteomic Analysis using Accurate Mass Tags. Gordon Anderson PNNL January 4-5, 2005

Proteomic Analysis using Accurate Mass Tags. Gordon Anderson PNNL January 4-5, 2005 Proteomic Analysis using Accurate Mass Tags Gordon Anderson PNNL January 4-5, 2005 Outline Accurate Mass and Time Tag (AMT) based proteomics Instrumentation Data analysis Data management Challenges 2 Approach

More information

Pinpointing phosphorylation sites using Selected Reaction Monitoring and Skyline

Pinpointing phosphorylation sites using Selected Reaction Monitoring and Skyline Pinpointing phosphorylation sites using Selected Reaction Monitoring and Skyline Christina Ludwig group of Ruedi Aebersold, ETH Zürich The challenge of phosphosite assignment Peptides Phosphopeptides MS/MS

More information

Research-grade Targeted Proteomics Assay Development: PRMs for PTM Studies with Skyline or, How I learned to ditch the triple quad and love the QE

Research-grade Targeted Proteomics Assay Development: PRMs for PTM Studies with Skyline or, How I learned to ditch the triple quad and love the QE Research-grade Targeted Proteomics Assay Development: PRMs for PTM Studies with Skyline or, How I learned to ditch the triple quad and love the QE Jacob D. Jaffe Skyline Webinar July 2015 Proteomics and

More information

MASCOT Search Results Interpretation

MASCOT Search Results Interpretation The Mascot protein identification program (Matrix Science, Ltd.) uses statistical methods to assess the validity of a match. MS/MS data is not ideal. That is, there are unassignable peaks (noise) and usually

More information

MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics

MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics With Unique QTRAP and TripleTOF 5600 System Technology Targeted peptide quantification is a rapidly growing application

More information

HRMS in Clinical Research: from Targeted Quantification to Metabolomics

HRMS in Clinical Research: from Targeted Quantification to Metabolomics A sponsored whitepaper. HRMS in Clinical Research: from Targeted Quantification to Metabolomics By: Bertrand Rochat Ph. D., Research Project Leader, Faculté de Biologie et de Médecine of the Centre Hospitalier

More information

Overview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System

Overview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System Investigating the use of the AB SCIEX TripleTOF 4600 LC/MS/MS System for High Throughput Screening of Synthetic Cannabinoids/Metabolites in Human Urine AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS

More information

Ultra Fast UHPLC-LCMSMS Method Development in Clinical Drug Monitoring

Ultra Fast UHPLC-LCMSMS Method Development in Clinical Drug Monitoring PO-CON1359E Ultra Fast UHPLC-LCMSMS Method Development in HPLC 2013 MASS-09 Anja Grüning 1 ; Brigitte Richrath 1 ; Klaus Bollig 2 ; Sven Vedder 1 ; Robert Ludwig 1 1 Shimadzu Europa GmbH, Duisburg, Germany;

More information

Improving the Metabolite Identification Process with Efficiency and Speed: LightSight Software for Metabolite Identification

Improving the Metabolite Identification Process with Efficiency and Speed: LightSight Software for Metabolite Identification Improving the Metabolite Identification Process with Efficiency and Speed: LightSight Software for Metabolite Identification Overview LightSight Software for Metabolite Identification is a complete environment

More information

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Hua-Fen Liu, Witold Woroniecki, Doina Caraiman, and Yves LeBlanc AB SCIEX, Foster City, USA One of the most challenging

More information

A Streamlined Workflow for Untargeted Metabolomics

A Streamlined Workflow for Untargeted Metabolomics A Streamlined Workflow for Untargeted Metabolomics Employing XCMS plus, a Simultaneous Data Processing and Metabolite Identification Software Package for Rapid Untargeted Metabolite Screening Baljit K.

More information

Application Note # MT-90 MALDI-TDS: A Coherent MALDI Top-Down-Sequencing Approach Applied to the ABRF-Protein Research Group Study 2008

Application Note # MT-90 MALDI-TDS: A Coherent MALDI Top-Down-Sequencing Approach Applied to the ABRF-Protein Research Group Study 2008 Bruker Daltonics Application Note # MT-90 MALDI-TDS: A Coherent MALDI Top-Down-Sequencing Approach Applied to the ABRF-Protein Research Group Study 2008 In the ABRF-PRG study 2008 [*] the ability to characterize

More information

Investigating Biological Variation of Liver Enzymes in Human Hepatocytes

Investigating Biological Variation of Liver Enzymes in Human Hepatocytes Investigating Biological Variation of Liver Enzymes in Human Hepatocytes MS/MS ALL with SWATH Acquisition on the TripleTOF Systems Xu Wang 1, Hui Zhang 2, Christie Hunter 1 1 AB SCIEX, USA, 2 Pfizer, USA

More information

DRUG METABOLISM. Drug discovery & development solutions FOR DRUG METABOLISM

DRUG METABOLISM. Drug discovery & development solutions FOR DRUG METABOLISM DRUG METABLISM Drug discovery & development solutions FR DRUG METABLISM Fast and efficient metabolite identification is critical in today s drug discovery pipeline. The goal is to achieve rapid structural

More information

Pep-Miner: A Novel Technology for Mass Spectrometry-Based Proteomics

Pep-Miner: A Novel Technology for Mass Spectrometry-Based Proteomics Pep-Miner: A Novel Technology for Mass Spectrometry-Based Proteomics Ilan Beer Haifa Research Lab Dec 10, 2002 Pep-Miner s Location in the Life Sciences World The post-genome era - the age of proteome

More information

Mascot Search Results FAQ

Mascot Search Results FAQ Mascot Search Results FAQ 1 We had a presentation with this same title at our 2005 user meeting. So much has changed in the last 6 years that it seemed like a good idea to re-visit the topic. Just about

More information

Introduction to Proteomics 1.0

Introduction to Proteomics 1.0 Introduction to Proteomics 1.0 CMSP Workshop Tim Griffin Associate Professor, BMBB Faculty Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s

More information

GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert1, Michel Lhermitte 1, Frederic Grisel 2 1

GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert1, Michel Lhermitte 1, Frederic Grisel 2 1 GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert, Michel Lhermitte, Frederic Grisel Laboratoire de Toxicologie & Génopathologie, CHRU Lille, France Waters Corporation, Guyancourt,

More information

A Quadrupole-Orbitrap Hybrid Mass Spectrometer Offers Highest Benchtop Performance for In-Depth Analysis of Complex Proteomes

A Quadrupole-Orbitrap Hybrid Mass Spectrometer Offers Highest Benchtop Performance for In-Depth Analysis of Complex Proteomes A Quadrupole-Orbitrap Hybrid Mass Spectrometer Offers Highest Benchtop Performance for In-Depth Analysis of Complex Proteomes Zhiqi Hao 1, Yi Zhang 1, Shannon Eliuk 1, Justin Blethrow 1, Dave Horn 1, Vlad

More information

Comparative LC-MS: A landscape of peaks and valleys

Comparative LC-MS: A landscape of peaks and valleys Proteomics 2008, 8, 731 749 DOI 10.1002/pmic.200700694 731 REVIEW Comparative LC-MS: A landscape of peaks and valleys Antoine H. P. America and Jan H. G. Cordewener Plant Research International, Wageningen

More information

Agilent 6000 Series LC/MS Solutions CONFIDENCE IN QUANTITATIVE AND QUALITATIVE ANALYSIS

Agilent 6000 Series LC/MS Solutions CONFIDENCE IN QUANTITATIVE AND QUALITATIVE ANALYSIS Agilent 6000 Series LC/MS Solutions CONFIDENCE IN QUANTITATIVE AND QUALITATIVE ANALYSIS AGILENT 6000 SERIES LC/MS SOLUTIONS UNRIVALED ACHIEVEMENT AT EVERY LEVEL The Agilent 6000 Series of LC/MS instruments

More information

itraq Tips and Tricks

itraq Tips and Tricks itraq Tips and Tricks Darryl Pappin Cold Spring Harbor Laboratory Patrick Emery Matrix Science Ltd. 1 Good morning. Unfortunately Darryl Pappin is unable to attend the Matrix Science workshop and the ASMS

More information

Mass Frontier 7.0 Quick Start Guide

Mass Frontier 7.0 Quick Start Guide Mass Frontier 7.0 Quick Start Guide The topics in this guide briefly step you through key features of the Mass Frontier application. Editing a Structure Working with Spectral Trees Building a Library Predicting

More information

La Protéomique : Etat de l art et perspectives

La Protéomique : Etat de l art et perspectives La Protéomique : Etat de l art et perspectives Odile Schiltz Institut de Pharmacologie et de Biologie Structurale CNRS, Université de Toulouse, Odile.Schiltz@ipbs.fr Protéomique et Spectrométrie de Masse

More information

Statistical Analysis Strategies for Shotgun Proteomics Data

Statistical Analysis Strategies for Shotgun Proteomics Data Statistical Analysis Strategies for Shotgun Proteomics Data Ming Li, Ph.D. Cancer Biostatistics Center Vanderbilt University Medical Center Ayers Institute Biomarker Pipeline normal shotgun proteome analysis

More information

Application Note # MS-14 Fast On-site Identification of Drugs with the mobile GC/MS system E²M

Application Note # MS-14 Fast On-site Identification of Drugs with the mobile GC/MS system E²M Bruker Daltonics Application Note # MS- Fast n-site Identification of Drugs with the mobile GC/MS system E²M For the detection of drugs a couple of quick tests are available which were used by the police

More information

Application Note FTMS-55 Laser/Desorption Ionization FT-ICR Mass Spectrometry as a Tool for Statistical Analysis of Crude Oils

Application Note FTMS-55 Laser/Desorption Ionization FT-ICR Mass Spectrometry as a Tool for Statistical Analysis of Crude Oils Application Note FTMS-55 Laser/Desorption Ionization FT-ICR Mass Spectrometry as a Tool for Statistical Analysis of Crude Oils Introduction Crude oil is an extremely complex mixture of hydrocarbons and

More information

Mass Spectrometry Based Proteomics

Mass Spectrometry Based Proteomics Mass Spectrometry Based Proteomics Proteomics Shared Research Oregon Health & Science University Portland, Oregon This document is designed to give a brief overview of Mass Spectrometry Based Proteomics

More information

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay PO-CON1484E Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching ASMS 014 TP85 David R. Baker 1, John Warrander 1, Neil Loftus 1, Simon Hird

More information

Electron Transfer Dissociation (ETD) Innovation with Integrity. Look Beneath the Surface. Ion Trap MS

Electron Transfer Dissociation (ETD) Innovation with Integrity. Look Beneath the Surface. Ion Trap MS Electron Transfer Dissociation (ETD) Look Beneath the Surface Innovation with Integrity Ion Trap MS Bruker Daltonics Leader in ETD Technology amazon Featuring ETD Since its commercial introduction in 2005,

More information

Challenges in Computational Analysis of Mass Spectrometry Data for Proteomics

Challenges in Computational Analysis of Mass Spectrometry Data for Proteomics Ma B. Challenges in computational analysis of mass spectrometry data for proteomics. SCIENCE AND TECHNOLOGY 25(1): 1 Jan. 2010 JOURNAL OF COMPUTER Challenges in Computational Analysis of Mass Spectrometry

More information

Industry Perspective: Advantages of Open Access and Walkup LC/ MS Supporting Protein Drug Discovery and Development

Industry Perspective: Advantages of Open Access and Walkup LC/ MS Supporting Protein Drug Discovery and Development Industry Perspective: Advantages of Open Access and Walkup LC/ MS Supporting Protein Drug Discovery and Development Dawn Stickle, Agilent Technologies Originally presented by Eric Fang, Novartis Overview

More information

aurora ICP-MS Innovation with Integrity The Science of Sensitivity ICP-MS

aurora ICP-MS Innovation with Integrity The Science of Sensitivity ICP-MS aurora ICP-MS The Science of Sensitivity Innovation with Integrity ICP-MS Powers the Business of Quantification aurora ICP-MS enhanced value package Bruker s ICP-MS is famous for its innovative and unique

More information

LC-MS/MS for Chromatographers

LC-MS/MS for Chromatographers LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS,

More information

EKSIGENT EKSPERT NANOLC 400. Unmatched flexibility for low flow LC/MS

EKSIGENT EKSPERT NANOLC 400. Unmatched flexibility for low flow LC/MS EKSIGENT EKSPERT NANOLC 4 Unmatched flexibility for low flow LC/MS EKSIGENT EKSPERT NANOLC 4 Focused ekspertise In 23, Eksigent changed the way scientists looked at nanoscale chromatography with the introduction

More information

Introduction to mass spectrometry (MS) based proteomics and metabolomics

Introduction to mass spectrometry (MS) based proteomics and metabolomics Introduction to mass spectrometry (MS) based proteomics and metabolomics Tianwei Yu Department of Biostatistics and Bioinformatics Rollins School of Public Health Emory University September 10, 2015 Background

More information

Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances

Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances TripleTOF 5600 + LC/MS/MS System with MasterView Software Adrian M. Taylor AB Sciex Concord, Ontario (Canada) Overview

More information

Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract

Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Lirui Qiao, 1 Rob Lewis, 2 Alex Hooper, 2 James Morphet, 2 Xiaojie Tan, 1 Kate

More information

Already said. Already said. Outlook. Look at LC-MS data. A look at data for quantitative analysis using MSight and Phenyx. What data for quantitation?

Already said. Already said. Outlook. Look at LC-MS data. A look at data for quantitative analysis using MSight and Phenyx. What data for quantitation? A look at data for quantitative analysis using MSight and Phenyx Pierre-Alain Binz Institut Suisse de Bioinformatique GeneBio SA Atelier Protéomique Quantitative 25-27 Juin 2007 La Grande Motte Already

More information

Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping

Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping For Conclusive Characterization of Biologics Deep Protein Characterization Is Crucial Pharmaceuticals have historically been small

More information

Shotgun Proteomic Analysis. Department of Cell Biology The Scripps Research Institute

Shotgun Proteomic Analysis. Department of Cell Biology The Scripps Research Institute Shotgun Proteomic Analysis Department of Cell Biology The Scripps Research Institute Biological/Functional Resolution of Experiments Organelle Multiprotein Complex Cells/Tissues Function Expression Analysis

More information

The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis

The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis Stephen Lock AB SCIEX Warrington (UK) Overview A rapid, robust, sensitive and specific LC-MS/MS method has been developed for

More information

Absolute quantification of low abundance proteins by shotgun proteomics

Absolute quantification of low abundance proteins by shotgun proteomics Absolute quantification of low abundance proteins by shotgun proteomics Dr. Stefanie Wienkoop www.proteomefactory.com In cooperation with: Max-Planck-Institut für Molekulare Pflanzenphysiologie Stable

More information

WATERS QUANTITATIVE ANALYSIS solutions

WATERS QUANTITATIVE ANALYSIS solutions More sensitivity. More speed. What more can you ask for in a complete quantitative analysis solution? WATERS QUANTITATIVE ANALYSIS solutions THE CHALLENGE OF QUANTITATIVE ANALYSIS Tandem mass spectrometry,

More information

Quantitative proteomics background

Quantitative proteomics background Proteomics data analysis seminar Quantitative proteomics and transcriptomics of anaerobic and aerobic yeast cultures reveals post transcriptional regulation of key cellular processes de Groot, M., Daran

More information

Learning Objectives:

Learning Objectives: Proteomics Methodology for LC-MS/MS Data Analysis Methodology for LC-MS/MS Data Analysis Peptide mass spectrum data of individual protein obtained from LC-MS/MS has to be analyzed for identification of

More information

Mass Spectrometry for Chemists and Biochemists

Mass Spectrometry for Chemists and Biochemists Erasmus Intensive Program SYNAPS Univ. of Crete - Summer 2007 Mass Spectrometry for Chemists and Biochemists Spiros A. Pergantis Assistant Professor of Analytical Chemistry Department of Chemistry University

More information

Mass Frontier Version 7.0

Mass Frontier Version 7.0 Mass Frontier Version 7.0 User Guide XCALI-97349 Revision A February 2011 2011 Thermo Fisher Scientific Inc. All rights reserved. Mass Frontier, Mass Frontier Server Manager, Fragmentation Library, Spectral

More information

MarkerView Software 1.2.1 for Metabolomic and Biomarker Profiling Analysis

MarkerView Software 1.2.1 for Metabolomic and Biomarker Profiling Analysis MarkerView Software 1.2.1 for Metabolomic and Biomarker Profiling Analysis Overview MarkerView software is a novel program designed for metabolomics applications and biomarker profiling workflows 1. Using

More information

Electrospray Ion Trap Mass Spectrometry. Introduction

Electrospray Ion Trap Mass Spectrometry. Introduction Electrospray Ion Source Electrospray Ion Trap Mass Spectrometry Introduction The key to using MS for solutions is the ability to transfer your analytes into the vacuum of the mass spectrometer as ionic

More information

Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2

Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2 Technical ote Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2 Alek. Dooley, Carmai Seto, esham Ghobarah, and Elliott B. Jones verview:

More information

Thermo Scientific SIEVE Software for Differential Expression Analysis

Thermo Scientific SIEVE Software for Differential Expression Analysis m a s s s p e c t r o m e t r y Thermo Scientific SIEVE Software for Differential Expression Analysis Automated, label-free, semi-quantitative analysis of proteins, peptides, and metabolites based on comparisons

More information

A Navigation through the Tracefinder Software Structure and Workflow Options. Frans Schoutsen Pesticide Symposium Prague 27 April 2015

A Navigation through the Tracefinder Software Structure and Workflow Options. Frans Schoutsen Pesticide Symposium Prague 27 April 2015 A Navigation through the Tracefinder Software Structure and Workflow Options Frans Schoutsen Pesticide Symposium Prague 27 April 2015 Kings day in The Netherlands 1 Index Introduction Acquisition, Method

More information

MassHunter for Agilent GC/MS & GC/MS/MS

MassHunter for Agilent GC/MS & GC/MS/MS MassHunter for Agilent GC/MS & GC/MS/MS Next Generation Data Analysis Software Presented by : Terry Harper GC/MS Product Specialist 1 Outline of Topics Topic 1: Introduction to MassHunter Topic 2: Data

More information

Agilent G2721AA/G2733AA Spectrum Mill MS Proteomics Workbench

Agilent G2721AA/G2733AA Spectrum Mill MS Proteomics Workbench Agilent G2721AA/G2733AA Spectrum Mill MS Proteomics Workbench Application Guide Agilent Technologies Notices Agilent Technologies, Inc. 2012 No part of this manual may be reproduced in any form or by any

More information

NUVISAN Pharma Services

NUVISAN Pharma Services NUVISAN Pharma Services CESI MS Now available! 1st CRO in Europe! At the highest levels of quality. LABORATORY SERVICES Equipment update STATE OF THE ART AT NUVISAN CESI MS Now available! 1st CRO in Europe!

More information

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction Advantages of Using Triple Quadrupole over Single Quadrupole Mass Spectrometry to Quantify and Identify the Presence of Pesticides in Water and Soil Samples André Schreiber AB SCIEX Concord, Ontario (Canada)

More information

API 3200 LC/MS/MS SYSTEM. Performance, productivity and value combined

API 3200 LC/MS/MS SYSTEM. Performance, productivity and value combined API 3200 LC/MS/MS SYSTEM Performance, productivity and value combined API 3200 LC/MS/MS SYSTEM Reliability, reproducibility, and confidence in your data. With an unmatched heritage of technological innovation

More information

Application of Structure-Based LC/MS Database Management for Forensic Analysis

Application of Structure-Based LC/MS Database Management for Forensic Analysis Cozette M. Cuppett and Michael P. Balogh Waters Corporation, Milford, MA USA Antony Williams, Vitaly Lashin and Ilya Troisky Advanced Chemistry Development, Toronto, ntario, Canada verview Application

More information

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs Thermo Scientific Prelude SPLC System FPO Making LC/MS accessible to clinical research and toxicology labs Specifically designed to make the power of LC/MS accessible Analysis of target compounds in complex

More information

Proteome Discoverer 1.3 Software: Enhanced Tools For Protein Identification

Proteome Discoverer 1.3 Software: Enhanced Tools For Protein Identification Proteome Discoverer 1.3 Software: Enhanced Tools For Protein Identification David Horn Proteomics Software Strategic Marketing Manager September 22, 2011 Hybrid Orbitrap Mass Spectrometers CID HCD ETD

More information

Proteomic data analysis for Orbitrap datasets using Resources available at MSI. September 28 th 2011 Pratik Jagtap

Proteomic data analysis for Orbitrap datasets using Resources available at MSI. September 28 th 2011 Pratik Jagtap Proteomic data analysis for Orbitrap datasets using Resources available at MSI. September 28 th 2011 Pratik Jagtap The Minnesota http://www.mass.msi.umn.edu/ Proteomics workflow Trypsin Protein Peptides

More information

Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem

Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem Kevin Van Cott, Associate Professor Dept. of Chemical and Biomolecular Engineering Nebraska

More information

Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System

Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System Olaf Scheibner, Maciej Bromirski, Thermo Fisher Scientific, Bremen, Germany

More information

Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM. Technical Overview.

Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM. Technical Overview. Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM Technical Overview Introduction The 5975A and B series mass selective detectors (MSDs) provide

More information

A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS

A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS AB SCIEX QTRAP 4500 LC/MS/MS System and Gerstel, Inc. MultiPurpose Sampler

More information

Using NIST Search with Agilent MassHunter Qualitative Analysis Software James Little, Eastman Chemical Company Sept 20, 2012.

Using NIST Search with Agilent MassHunter Qualitative Analysis Software James Little, Eastman Chemical Company Sept 20, 2012. Using NIST Search with Agilent MassHunter Qualitative Analysis Software James Little, Eastman Chemical Company Sept 20, 2012 Introduction Screen captures in this document were taken from MassHunter B.05.00

More information

Agilent 6300 Ion Trap LC/MS Systems Quick Start Guide

Agilent 6300 Ion Trap LC/MS Systems Quick Start Guide Agilent 6300 Ion Trap LC/MS Systems Quick Start Guide A guide to help you get started with the Agilent 6300 Series Ion Trap LC/MS system What is the Agilent 6300 Series Ion Trap LC/MS System? 2 Where to

More information

Evaluation of LC-MS data for the absolute quantitative analysis of marker proteins

Evaluation of LC-MS data for the absolute quantitative analysis of marker proteins Evaluation of LC-MS data for the absolute quantitative analysis of marker proteins Nathanaël Delmotte 1, Bettina Mayr 1, Andreas Leinenbach 1, Knut Reinert 2, Oliver Kohlbacher 3, Christoph Klein 4, and

More information

Introduction to Proteomics

Introduction to Proteomics Introduction to Proteomics Åsa Wheelock, Ph.D. Division of Respiratory Medicine & Karolinska Biomics Center asa.wheelock@ki.se In: Systems Biology and the Omics Cascade, Karolinska Institutet, June 9-13,

More information

Chemical Analysis. Simulated Distillation Analyzers. A full range of simulated Distillation Analysis Solution. Gas Chromatography.

Chemical Analysis. Simulated Distillation Analyzers. A full range of simulated Distillation Analysis Solution. Gas Chromatography. Chemical Analysis Simulated Distillation Analyzers A full range of simulated Distillation Analysis Solution think forward Gas Chromatography Bruker Simulated Distillation Analyzers Bruker s range of Simulated

More information