QUALITY AND SAFETY TESTING

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1 QUALITY AND SAFETY TESTING Large scale of solutions for the identification and rapid detection of micro-organisms. Easier investment in molecular techniques Frédéric BAR, Key Account Manager

2 b2 b3b4 Quality & Safety Testing b5 Une histoire: l origine de la vie Un saut: des technologies accessibles pour le contrôle de routine 1981 Applied Biosystems is founded AB introduce s 380A DNA synthe sizer AB introduce s ABI PRISM 3700 DNA Analyzer: accelerating completion of human genome draft AB DNA analyzers and reagents used to identify 30,000 human genes and discover 10 million+ unique SNPs AB introduce s first First commercial PCR AB DNA analyzers AB DNA analyzers, gas-pha se protein enzyme and thermal and reagents real-time PCR, and sequencer: the 470A. cycler systems enable draft protein ID systems introduced. version of the used to sequence human genome the SARS virus, and sequence. to develop diagnostic te sts Sep-06 Confidential

3 Diapositive 2 b2 Linda - I shortened the words...i really like this slide! Cathy burzikcm; 09/01/2005 b3 burzikcm; 09/01/2005 b4 burzikcm; 09/01/2005 b5 Linda- I really like this slide...i just shortened it. Cathy burzikcm; 09/01/2005

4 Applied Markets Market Segments Forensic Human Identification Quality & Safety Testing (QST) Pharma & Food & Environmental Biosecurity On-line pathogen detection Toxicology Microbial Identification Metabolite analysis 3 15-Sep-06 Confidential

5 Marché de la détection et de l identification Market Value of Food Testing Pathogen Tests by Method USD Millions 1,500 1,309 Market size and growth In 2003 the market size for food pathogen testing s $490M 100% 80 $420M $490M $968M Traditional 1, Pathogen 968 Routine $100M (20%) is comprised of molecular methods. Molecular methods are the fastest growing of all methods used for pathogen testing and are expected to grow to over $360M in 2008 ( CAGR = 29%) Immunoassay Molecular % of Total 4 15-Sep-06 Confidential

6 Notre Stratégie Bioinformatics Content Celera Discovery Systems TM Platform Assays Reagents and Assays Instruments Results! Platforms 5 15-Sep-06 Confidential Services Services & Informatics

7 Why DNA testing? Antibody reactions DNA RNA Proteins Metabolic reactions Genotypic vs. Phenotypic Fatty acid profiles 6 15-Sep-06 Confidential

8 Microbiologie Alimentaire De Pasteur à la PCR en temps réel 4 différents TaqMan Pathogen Detection Kits: Listeria monocytogenes (hlya gene) Salmonella enterica (hila gene) Escherichia coli 0157:H7(eaeA gene) Campylobacter jejuni (ccon gene) Environe ment Legionella spp et pneumophila Pharma et Cosmétique QC Staphylococcus aureus Pseudomonas aeruginosa ADN résiduel Mycoplasme Vins et Bières Virus Brettanomyces (Prédel) Grippe Aviaire H5N1 Rotavirus, Norovirus, 7 15-Sep-06 Confidential

9 De Pasteur au séquençage MicroSEQ: Solution pour l identification Bactérie/moisissure Identification à 100% 8 15-Sep-06 Confidential

10 MicroSeq System Workflow Starting material: sample from colony or pure culture DNA isolation with PrepMan Ultra kit 30 min PCR with fast MicroSeq PCR kit Purification of PCR product Sequencing reaction with MicroSeq sequencing kit (2 reactions / sample: forward and reverse) Purification of sequencing product Capillary electrophoresis on Genetic Analyzer 20 min 50 min 2 hours Data analysis in MicroSeq ID software 10 min 9 15-Sep-06 Confidential Library Search Report (Hit List)

11 Gamme d instrument la plus large Haut débit Developpement 5 couleurs 384 puits Prix New QC 3 couleurs 48 puits pilotable par Internet Ecran tactile Sep-06 Confidential 4-5 couleurs 96 puits Caractéristiques Laser Robotisé Product Price List ( ) StepOne Fast HT HT LDA HT R

12 Nouvelle stratégie d investissement pour les laboratoires d analyses Organisation Multi travail Screening Haut débit TTR Spécificité ADN Investissement initial lourd Méthode traditionnelle Coût analyse élevé Certification Méthode moléculaire Acquisition connaissance Sep-06 Confidential

13 Investissement initial Haut débit Diagnostic m2000rt Developpement 7500 Fast 384- Well Feature Loaded 7900HT 7900HTA 7300 QC 4-Color Color 7500 Prix 3-Color StepOne Single Reporter Sep-06 Confidential Caractéristiques Product Price List ( ) StepOne Fast HT HT LDA HT R

14 Investissement initial Haut débit 1 StepOne m.a.d Analyses/an Diagnostic m2000rt Developpement 7500 Fast 384- Well Feature Loaded 7900HT 7900HTA 7300 QC 4-Color Color 7500 Volume Prix Analyses 3-Color StepOne Single Reporter Sep-06 Confidential Caractéristiques

15 Coût d analyse Segmentation des clients Industries Pharma Cosméto IAA RHF Techni que moléculaire = protection de l a marque Concentration des pathogènes sur 1 plaque Moins de lecture = réduction du te mps de travail Automatisation Sep-06 Confidential

16 Applied Biosystems Efficient Route to Success Quality & Safety Testing Un support complet-formations-séminaires Screen Validated Targets Validate with TaqMan High Quality DNA Determine faster& accurate microbial burden Genetic analysers Sep-06 Confidential

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