Vol. 17 No Journal of Jimei University Natural Science Jul % AFLP % Female Special Band FSB 178

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1 Vol 17 No Journal of Jimei University Natural Science Jul AFLP F1 AFLP 93 54% 86 23% 8 AFLP 456 Female Special Band FSB 178 Male Special Band MSB 187 Mutual Band MuB % FSB % MSB % MuB % FSB % MSB % MuB FSB MSB 33 0% 28 6% AFLP AFLP S A Amplified Fragment Length Polymorphism Analysis on Hybrid Family Between Nibea albiflora and Larimichthys crocea SUI Ban-liang 1 CAI Ming-yi 1 2 LIU Ying 1 2 WANG Zhi-yong Fisheries College Jimei University Xiamen China 2 Key Laboratory of Helthy Mariculture for the East China Sea Ministry of Agriculture Xiamen China Abstract Hybridization experiments between Nibea albiflora and Larimichthys crocea were carried out The average fertilization rate and the hatching rate of the hybrid cross were 93 54% and 86 23% respectively However the hybrids were all dead within one week after hatching To elucidate the genetic composition of the hybrids 32 larvae from a hybrid family NL was investigated by using AFLP technology Totally 456 AFLP bands were detected in parents and progenies which included 178 female parent - specific bands FSB 187 male parent specific bands MSB and 91 mutual bands MuB All of the fragments were detected in the hybrid progenies including % FSB % MSB and % MuB MuB was found in all the progenies while other bands were segregated in the progenies with 33 0% of FSB and 28 6% of MSB distorted segregation The AFLP results suggested that the larvae of N albiflora L crocea were the true hybrids containing whole nuclear genome of both parents so they J A zywang@ jmu edu cn

2 are applicable to construct the genetic maps of female N albiflora and that of male L crocea Key words Nibea albiflora Larimichthys crocea AFLP hybridization larva Nibea albiflora Sciaenidae Nibea 12 Larimichthys crocea Larimichthys F1 F1 15 AFLP F1 F LRH - A3 0 5μg /kg 1μg /kg % 95% AFLP NL NF LM DNA DNA K - / 14 DNA DNA DNA OD 260 OD 280 DNA 50 ng /μl 4 DNA AFLP AFLP Wang 16 Invitrogen E - AAG /M - CAG E - AGG /M - CTG E - AAG /M - CTC E - AGG /M - CTC E - AAC /M - CAG E - ACC /M - CAA E - AAG /M - CAA E - AGG /M - CAA E - GACTGCGTACCAATTC M - GAT- GAGTCCTGAGTAA AFLP 6% AFLP AnalyzerVer UPGMA S ij = 2 N ij / N i + N j N ij i j N i N j i j

3 4 AFLP 243 D = - Ln S S NL NN 1 LL Tab 1 Fertilization rate hatching rate 1 aberration rate and survival rate of the hybrid and control family /% /% /% 1 Fertilization Hatching Aberration Family rate rate rate 0 NL ± ± ± AFLP NN ± ± ± LL ± ± ± 0 00 AFLP NL NN 8 LL NL n = 32 Notes NL means N albiflora and L crocea NN means N albiflora and N albiflora LL means L crocea and L crocea 456 FSB 178 MSB NL Tab 2 Numbers of amplified bands with 8 sets of selective AFLP primers in the NL hybrid family 2 1 No 2 3 AFLP F1 AFLP 3 Primer Total MSB FSB MuB MSB E - AGG /M - CTG FSB MuB % FSB 89 E - AAG /M - CAG % MSB E - AAG /M - CTC % MuB % FSB 98 E - AGG /M - CTC % MSB E - AAC /M - CAG % MuB E - ACC /M - CAA FSB MSB 33 0% E - AAG /M - CAA 28 6% NL FSB MSB E - AGG /M - CAA 0 62 ± ± 0 19 Total Tab 3 3 AFLP NL Inheritance and segregation pattern of AFLP markers in the hybrid F1 of N albiflora L crocea / Number of loci Type of loci /No /% Non-segregated loci /% Segregated loci /% Distorted segregated loci FSB MSB MuB

4 AFLP UPGMA 2 4 Tab 4 Family F1 The average genetic distance above diagonal and genetic similarity below diagonal between F1 and their parents in the hybrid family of N albiflora L crocea Hybrid N albiflora female L crocea male Hybrid N albiflora female L crocea male % F1 8 AFLP 456 MSB 187 F 1

5 4 AFLP 245 FSB 178 MuB 91 AFLP % FSB % MSB % MuB FSB MSB AFLP % FSB % MSB % MuB FSB MSB 33 0% 28 6% 14 AFLP Oncorhynchus mykiss 20 Haliotis hannai 21 Ictalurus punctatus 22 Crassostrea virginica 23 Pinctada martensii 24 Oreochromis niloticus SSR 27 F1 1 F1 1 J D J J J

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