GENEWIZ, Inc. DNA Sequencing Service Details for USC Norris Comprehensive Cancer Center DNA Core
|
|
|
- Arnold Barton
- 7 years ago
- Views:
Transcription
1 DNA Sequencing Services Pre-Mixed o Provide template and primer, mixed into the same tube* Pre-Defined o Provide template and primer in separate tubes* Custom o Full-service for samples with unknown concentration o Sample types include: Plasmids* PCR products (purified and unpurified)* Bacterial clones (colonies and glycerol stock)* Phage with circular genome (supernatant and plaque)* BAC DNA* o -dependent services include: Measure template concentration and optimize for sequencing Amplify template from clones: Bacterial colonies Glycerol stock Phage plaque Phage supernatant Clean-up crude PCR products with one band using ExoSAP-IT Select unpurified PCR product as the DNA type and PCR clean-up as the Special Request when placing DNA sequencing order for this service option *Please prepare all DNA sequencing samples according to Sample Submission Guidelines Note: GENEWIZ Universal s available for all DNA sequencing services, free of charge DNA Sequencing Results & Support Fast & Convenient: Data reported the morning following sample pick-up; results are provided via in an easily accessible web link o Monday Friday: Results reported by 10:00 a.m. o Saturday: Results reported by 12:00 p.m. High Quality: Read length bp, >700bp Phred20; Quality Score and Contiguous Read Length (CRL) provided with each read Best-in-Class Proprietary Protocols: Difficult template sequencing, including GC-rich DNA and RNAi Online Ordering & Data Retrieval: Accessible 24/7 Technical Support: Award-winning, easily accessible technical support available to answer questions and help with any troubleshooting needs, Monday Friday, 8:00 a.m. 8:00 p.m. (ET)
2 GENEWIZ Free Universal s T7 20 mer 5'-d(TAA TAC GAC TCA CTA TAG GG)-3' T3 20 mer 5'-d(ATT AAC CCT CAC TAA AGG GA)-3' SP6 19 mer 5'-d(GAT TTA GGT GAC ACT ATA G)-3' BGHR 18 mer 5'-d(TAG AAG GCA CAG TCG AGG)-3' M13F(-21) 18 mer 5'-d(TGT AAA ACG ACG GCC AGT)-3' M13F(-47) 24 mer 5'-d(CGC CAG GGT TTT CCC AGT CAC GAC)-3' M13R 17 mer 5'-d(CAG GAA ACA GCT ATG AC)-3' T7 Term 19 mer 5'-d(GCT AGT TAT TGC TCA GCG G)-3' CMV-Forward 21 mer 5'-d(CGC AAA TGG GCG GTA GGC GTG)-3' 3AOX1 21 mer 5'-d(GCA AAT GGC ATT CTG ACA TCC)-3' 5AOX1 21 mer 5'-d(GAC TGG TTC CAA TTG ACA AGC)-3' 5GEX 23 mer 5'-d(GGG CTG GCA AGC CAC GTT TGG TG)-3' 3GEX 23 mer 5'-d(CCG GGA GCT GCA TGT GTC AGA GG)-3' pfastbacf 20 mer 5'-d(GGA TTA TTC ATA CCG TCC CA)-3' pfastbacr 20 mer 5'-d(CAA ATG TGG TAT GGC TGA TT)-3' pbad Forward 20 mer 5'-d(ATG CCA TAG CAT TTT TAT CC)-3' pbad Reverse 18 mer 5'-d(GAT TTA ATC TGT ATC AGG)-3' M13F 18 mer 5'-d(GGT TTT CCC AGT CAC GAC)-3' T7 EEV 22 mer 5'-d(ATG TCG TAA TAA CCC CGC CCC G)-3' V5 21 mer 5'-d(ACC GAG GAG AGG GTT AGG GAT)-3' Myc 24 mer 5'-d(GCA TCA ATG CAG AAG CTG ATC TCA)-3' Neo-F 20 mer 5'-d(CGT TGG C TA CCC GTG ATA TT)-3 Neo-R 18 mer 5'-d(TGG ATA CTT TCT CGG CAG)-3' EGFP-C 22 mer 5'-d(CAT GGT CCT GCT GGA GTT CGT G)-3' EGFP-N 21 mer 5'-d(CGT CGC CGT CCA GCT CGA CCA)-3' SV40pA-R 20 mer 5'-d(GAA ATT TGT GAT GCT ATT GC)-3' SV40-Promoter 20 mer 5'-d(TAT TTA TGC AGA GGC CGA GG)-3'
3 DNA Sequencing Sample Submission Guidelines Three Easy Steps for Sample Submission: 1. Orders with <48 samples: Use 8-strip PCR tubes to streamline preparation and processing; please label tubes with your initials and the sample number. Orders with >48 samples: Receive a discount by using a 96-well PCR plate and arranging samples vertically (A1 H1). 2. Dilute sequencing primer to 5M (pmol/l) using water; each reaction requires 5l. GENEWIZ Universal s are also available, at no additional charge. 3. Use specifications in the following tables to prepare samples based on your order type. Sample Preparation (Pre-Mixed; Pre-Defined; Custom) Pre-Mixed: In the same tube, mix template (10l) and primer (5l) according to the following specifications: DNA Length in 10l Total Picomoles Pre-Mixed Volume* ( + ) <6 kb ~50ng/l ~500ng Plasmid 6-10 kb ~80ng/l ~800ng 25pmol 15l >10 kb ~100ng/l ~1000ng PCR Product <500 bp ~1ng/l ~10ng bp ~2ng/l ~20ng bp ~4ng/l ~40ng bp ~6ng/l ~60ng >4000 bp 25pmol *If using a GENEWIZ Universal, please submit the required amount of template in 10l 15l
4 Any Size GENEWIZ, Inc. DNA Sequencing Service Details Pre-Defined: In separate tubes, provide template (10l) and primer (5l) according to the following specifications: Plasmid DNA Length in 10l <6 kb ~50ng/l ~500ng 6-10 kb ~80ng/l ~800ng >10 kb ~100ng/l ~1000ng M (pmol/10l) 10l 5l 5l PCR Product <500 bp ~1ng/l ~10ng bp ~2ng/l ~20ng bp ~4ng/l ~40ng bp ~6ng/l ~60ng >4000 bp *See Technical Notes for tips on how to purify PCR products 10l 5l 5l Custom: GENEWIZ will determine template concentration, optimize for sequencing, and mix with primer; if available, please provide template concentration according to the following specifications: DNA Length (recommended) M (pmol/10l) Plasmid Any Size Unknown At least 500ng 10l 5l 5l PCR Product Any Size Unknown At least 40ng 10l 5l 5l *Please submit the same amount of DNA required for purified PCR products when submitting unpurified PCR products For more Sample Submission Guidelines, including details for Bacteria & Phage, Tubes & Plates, and Technical Notes, please click here.
5 GENEWIZ Gene Synthesis Gene synthesis is an efficient and cost-effective alternative to molecular cloning for custom gene production. GENEWIZ can synthesize codon-optimized cdna, gene variants, artificially designed DNA, or any other sequence for you research. Provide a nucleotide or amino acid, and GENEWIZ will ship your gene cloned into the of your choice. GENEWIZ gene synthesis services include: Full Service Fast & Reliable Turnaround Expert Project Management Personalized Consultation & Support Intellectual Property Security Standard deliverables include: 2-5g of lyophilized containing your desired synthetic construct Certificate of Analysis (COA), including restriction digest Sequence trace data with alignment Sequence of synthetic gene alone and in vector Fast turnaround time: Synthetic Gene Length Standard Delivery Time* <1.5 kb 8-10 Business Days kb Business Days 3-5 kb Business Days 5-6 kb Business Days >6 kb Custom Quote For more information about GENEWIZ Gene Synthesis, please click here.
DNA Sample preparation and Submission Guidelines
DNA Sample preparation and Submission Guidelines Requirements: Please submit samples in 1.5ml microcentrifuge tubes. Fill all the required information in the Eurofins DNA sequencing order form and send
(http://genomes.urv.es/caical) TUTORIAL. (July 2006)
(http://genomes.urv.es/caical) TUTORIAL (July 2006) CAIcal manual 2 Table of contents Introduction... 3 Required inputs... 5 SECTION A Calculation of parameters... 8 SECTION B CAI calculation for FASTA
(A) Microarray analysis was performed on ATM and MDM isolated from 4 obese donors.
Legends of supplemental figures and tables Figure 1: Overview of study design and results. (A) Microarray analysis was performed on ATM and MDM isolated from 4 obese donors. After raw data gene expression
Gene Synthesis 191. Mutagenesis 194. Gene Cloning 196. AccuGeneBlock Service 198. Gene Synthesis FAQs 201. User Protocol 204
Gene Synthesis 191 Mutagenesis 194 Gene Cloning 196 AccuGeneBlock Service 198 Gene Synthesis FAQs 201 User Protocol 204 Gene Synthesis Overview Gene synthesis is the most cost-effective way to enhance
10 µg lyophilized plasmid DNA (store lyophilized plasmid at 20 C)
TECHNICAL DATA SHEET BIOLUMINESCENCE RESONANCE ENERGY TRANSFER RENILLA LUCIFERASE FUSION PROTEIN EXPRESSION VECTOR Product: prluc-c Vectors Catalog number: Description: Amount: The prluc-c vectors contain
SERVICES CATALOGUE WITH SUBMISSION GUIDELINES
SERVICES CATALOGUE WITH SUBMISSION GUIDELINES 3921 Montgomery Road Cincinnati, Ohio 45212 513-841-2428 www.agctsequencing.com CONTENTS Welcome Dye Terminator Sequencing DNA Sequencing Services - Full Service
Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation
BDGP Resources Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation For recovery of sequences flanking PZ, PlacW and PEP elements E. Jay Rehm Berkeley Drosophila Genome Project I.
UNIVERSITETET I OSLO Det matematisk-naturvitenskapelige fakultet
1 UNIVERSITETET I OSLO Det matematisk-naturvitenskapelige fakultet Exam in: MBV4010 Arbeidsmetoder i molekylærbiologi og biokjemi I MBV4010 Methods in molecular biology and biochemistry I Day of exam:.
pcas-guide System Validation in Genome Editing
pcas-guide System Validation in Genome Editing Tagging HSP60 with HA tag genome editing The latest tool in genome editing CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible
Supplementary Online Material for Morris et al. sirna-induced transcriptional gene
Supplementary Online Material for Morris et al. sirna-induced transcriptional gene silencing in human cells. Materials and Methods Lentiviral vector and sirnas. FIV vector pve-gfpwp was prepared as described
Table S1. Related to Figure 4
Table S1. Related to Figure 4 Final Diagnosis Age PMD Control Control 61 15 Control 67 6 Control 68 10 Control 49 15 AR-PD PD 62 15 PD 65 4 PD 52 18 PD 68 10 AR-PD cingulate cortex used for immunoblot
Introduction to Perl Programming Input/Output, Regular Expressions, String Manipulation. Beginning Perl, Chap 4 6. Example 1
Introduction to Perl Programming Input/Output, Regular Expressions, String Manipulation Beginning Perl, Chap 4 6 Example 1 #!/usr/bin/perl -w use strict; # version 1: my @nt = ('A', 'C', 'G', 'T'); for
Mutations and Genetic Variability. 1. What is occurring in the diagram below?
Mutations and Genetic Variability 1. What is occurring in the diagram below? A. Sister chromatids are separating. B. Alleles are independently assorting. C. Genes are replicating. D. Segments of DNA are
pcmv6-neo Vector Application Guide Contents
pcmv6-neo Vector Application Guide Contents Package Contents and Storage Conditions... 2 Product Description... 2 Introduction... 2 Production and Quality Assurance... 2 Methods... 3 Other required reagents...
The p53 MUTATION HANDBOOK
The p MUTATION HANDBOOK Version 1. /7 Thierry Soussi Christophe Béroud, Dalil Hamroun Jean Michel Rubio Nevado http://p/free.fr The p Mutation HandBook By T Soussi, J.M. Rubio-Nevado, D. Hamroun and C.
Supplementary Information. Binding region and interaction properties of sulfoquinovosylacylglycerol (SQAG) with human
Supplementary Information Binding region and interaction properties of sulfoquinovosylacylglycerol (SQAG) with human vascular endothelial growth factor 165 revealed by biosensor based assays Yoichi Takakusagi
Molecular analyses of EGFR: mutation and amplification detection
Molecular analyses of EGFR: mutation and amplification detection Petra Nederlof, Moleculaire Pathologie NKI Amsterdam Henrique Ruijter, Ivon Tielen, Lucie Boerrigter, Aafke Ariaens Outline presentation
Next Generation Sequencing
Next Generation Sequencing 38. Informationsgespräch der Blutspendezentralefür Wien, Niederösterreich und Burgenland Österreichisches Rotes Kreuz 22. November 2014, Parkhotel Schönbrunn Die Zukunft hat
Hands on Simulation of Mutation
Hands on Simulation of Mutation Charlotte K. Omoto P.O. Box 644236 Washington State University Pullman, WA 99164-4236 omoto@wsu.edu ABSTRACT This exercise is a hands-on simulation of mutations and their
Inverse PCR and Sequencing of P-element, piggybac and Minos Insertion Sites in the Drosophila Gene Disruption Project
Inverse PCR and Sequencing of P-element, piggybac and Minos Insertion Sites in the Drosophila Gene Disruption Project Protocol for recovery of sequences flanking insertions in the Drosophila Gene Disruption
Part ONE. a. Assuming each of the four bases occurs with equal probability, how many bits of information does a nucleotide contain?
Networked Systems, COMPGZ01, 2012 Answer TWO questions from Part ONE on the answer booklet containing lined writing paper, and answer ALL questions in Part TWO on the multiple-choice question answer sheet.
Title : Parallel DNA Synthesis : Two PCR product from one DNA template
Title : Parallel DNA Synthesis : Two PCR product from one DNA template Bhardwaj Vikash 1 and Sharma Kulbhushan 2 1 Email: vikashbhardwaj@ gmail.com 1 Current address: Government College Sector 14 Gurgaon,
ANALYSIS OF A CIRCULAR CODE MODEL
ANALYSIS OF A CIRCULAR CODE MODEL Jérôme Lacan and Chrstan J. Mchel * Laboratore d Informatque de Franche-Comté UNIVERSITE DE FRANCHE-COMTE IUT de Belfort-Montbélard 4 Place Tharradn - BP 747 5 Montbélard
TITRATION OF raav (VG) USING QUANTITATIVE REAL TIME PCR
Page 1 of 5 Materials DNase digestion buffer [13 mm Tris-Cl, ph7,5 / 5 mm MgCl2 / 0,12 mm CaCl2] RSS plasmid ptr-uf11 SV40pA Forward primer (10µM) AGC AAT AGC ATC ACA AAT TTC ACA A SV40pA Reverse Primer
Gene Finding CMSC 423
Gene Finding CMSC 423 Finding Signals in DNA We just have a long string of A, C, G, Ts. How can we find the signals encoded in it? Suppose you encountered a language you didn t know. How would you decipher
ANALYSIS OF GROWTH HORMONE IN TENCH (TINCA TINCA) ANALÝZA RŮSTOVÉHO HORMONU LÍNA OBECNÉHO (TINCA TINCA)
ANALYSIS OF GROWTH HORMONE IN TENCH (TINCA TINCA) ANALÝZA RŮSTOVÉHO HORMONU LÍNA OBECNÉHO (TINCA TINCA) Zrůstová J., Bílek K., Baránek V., Knoll A. Ústav morfologie, fyziologie a genetiky zvířat, Agronomická
Cloning, sequencing, and expression of H.a. YNRI and H.a. YNII, encoding nitrate and nitrite reductases in the yeast Hansenula anomala
Cloning, sequencing, and expression of H.a. YNRI and H.a. YNII, encoding nitrate and nitrite reductases in the yeast Hansenula anomala -'Pablo García-Lugo 1t, Celedonio González l, Germán Perdomo l, Nélida
NimbleGen SeqCap EZ Library SR User s Guide Version 3.0
NimbleGen SeqCap EZ Library SR User s Guide Version 3.0 For life science research only. Not for use in diagnostic procedures. Copyright 2011 Roche NimbleGen, Inc. All Rights Reserved. Editions Version
Supplemental Data. Short Article. PPARγ Activation Primes Human Monocytes. into Alternative M2 Macrophages. with Anti-inflammatory Properties
Cell Metabolism, Volume 6 Supplemental Data Short Article PPARγ Activation Primes Human Monocytes into Alternative M2 Macrophages with Anti-inflammatory Properties M. Amine Bouhlel, Bruno Derudas, Elena
Marine Biology DEC 2004; 146(1) : 53-64 http://dx.doi.org/10.1007/s00227-004-1423-6 Copyright 2004 Springer
Marine Biology DEC 2004; 146(1) : 53-64 http://dx.doi.org/10.1007/s00227-004-1423-6 Copyright 2004 Springer Archimer http://www.ifremer.fr/docelec/ Archive Institutionnelle de l Ifremer The original publication
Y-chromosome haplotype distribution in Han Chinese populations and modern human origin in East Asians
Vol. 44 No. 3 SCIENCE IN CHINA (Series C) June 2001 Y-chromosome haplotype distribution in Han Chinese populations and modern human origin in East Asians KE Yuehai ( `º) 1, SU Bing (3 Á) 1 3, XIAO Junhua
On Covert Data Communication Channels Employing DNA Recombinant and Mutagenesis-based Steganographic Techniques
On Covert Data Communication Channels Employing DNA Recombinant and Mutagenesis-based Steganographic Techniques MAGDY SAEB 1, EMAN EL-ABD 2, MOHAMED E. EL-ZANATY 1 1. School of Engineering, Computer Department,
Module 6: Digital DNA
Module 6: Digital DNA Representation and processing of digital information in the form of DNA is essential to life in all organisms, no matter how large or tiny. Computing tools and computational thinking
Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR. Protocol
Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR Protocol 31 January 2011 Joint Research Centre Institute for Health and Consumer Protection Molecular Biology and Genomics
All commonly-used expression vectors used in the Jia Lab contain the following multiple cloning site: BamHI EcoRI SmaI SalI XhoI_ NotI
2. Primer Design 2.1 Multiple Cloning Sites All commonly-used expression vectors used in the Jia Lab contain the following multiple cloning site: BamHI EcoRI SmaI SalI XhoI NotI XXX XXX GGA TCC CCG AAT
The making of The Genoma Music
242 Summary Key words Resumen Palabras clave The making of The Genoma Music Aurora Sánchez Sousa 1, Fernando Baquero 1 and Cesar Nombela 2 1 Department of Microbiology, Ramón y Cajal Hospital, and 2 Department
Chapter 9. Applications of probability. 9.1 The genetic code
Chapter 9 Applications of probability In this chapter we use the tools of elementary probability to investigate problems of several kinds. First, we study the language of life by focusing on the universal
Drosophila NK-homeobox genes
Proc. Natl. Acad. Sci. USA Vol. 86, pp. 7716-7720, October 1989 Biochemistry Drosophila NK-homeobox genes (NK-1, NK-2,, and DNA clones/chromosome locations of genes) YONGSOK KIM AND MARSHALL NIRENBERG
Transmembrane Signaling in Chimeras of the E. coli Chemotaxis Receptors and Bacterial Class III Adenylyl Cyclases
Transmembrane Signaling in Chimeras of the E. coli Chemotaxis Receptors and Bacterial Class III Adenylyl Cyclases Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Eberhard Karls Universität
Five-minute cloning of Taq polymerase-amplified PCR products
TOPO TA Cloning Version R 8 April 2004 25-0184 TOPO TA Cloning Five-minute cloning of Taq polymerase-amplified PCR products Catalog nos. K4500-01, K4500-40, K4510-20, K4520-01, K4520-40, K4550-01, K4550-40,
Institutional Partnership Program
GENEWIZ Outsourcing Services Institutional Partnership Program Solid Science. Superior Service. DNA Sequencing Partners to Fuel Your Success Institutions whose success depends on significant life science
DISSERTATIONES MEDICINAE UNIVERSITATIS TARTUENSIS 108
DISSERTATIONES MEDICINAE UNIVERSITATIS TARTUENSIS 108 DISSERTATIONES MEDICINAE UNIVERSITATIS TARTUENSIS 108 THE INTERLEUKIN-10 FAMILY CYTOKINES GENE POLYMORPHISMS IN PLAQUE PSORIASIS KÜLLI KINGO TARTU
Molecular chaperones involved in preprotein. targeting to plant organelles
Molecular chaperones involved in preprotein targeting to plant organelles Dissertation der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Christine Fellerer München 29.
were demonstrated to be, respectively, the catalytic and regulatory subunits of protein phosphatase 2A (PP2A) (29).
JOURNAL OF VIROLOGY, Feb. 1992, p. 886-893 0022-538X/92/020886-08$02.00/0 Copyright C) 1992, American Society for Microbiology Vol. 66, No. 2 The Third Subunit of Protein Phosphatase 2A (PP2A), a 55- Kilodalton
2006 7.012 Problem Set 3 KEY
2006 7.012 Problem Set 3 KEY Due before 5 PM on FRIDAY, October 13, 2006. Turn answers in to the box outside of 68-120. PLEASE WRITE YOUR ANSWERS ON THIS PRINTOUT. 1. Which reaction is catalyzed by each
Coding sequence the sequence of nucleotide bases on the DNA that are transcribed into RNA which are in turn translated into protein
Assignment 3 Michele Owens Vocabulary Gene: A sequence of DNA that instructs a cell to produce a particular protein Promoter a control sequence near the start of a gene Coding sequence the sequence of
The DNA-"Wave Biocomputer"
The DNA-"Wave Biocomputer" Peter P. Gariaev (Pjotr Garjajev)*, Boris I. Birshtein*, Alexander M. Iarochenko*, Peter J. Marcer**, George G. Tertishny*, Katherine A. Leonova*, Uwe Kaempf ***. * Institute
http://www.life.umd.edu/grad/mlfsc/ DNA Bracelets
http://www.life.umd.edu/grad/mlfsc/ DNA Bracelets by Louise Brown Jasko John Anthony Campbell Jack Dennis Cassidy Michael Nickelsburg Stephen Prentis Rohm Objectives: 1) Using plastic beads, construct
EU Reference Laboratory for E. coli Department of Veterinary Public Health and Food Safety Unit of Foodborne Zoonoses Istituto Superiore di Sanità
Identification and characterization of Verocytotoxin-producing Escherichia coli (VTEC) by Real Time PCR amplification of the main virulence genes and the genes associated with the serogroups mainly associated
RT-PCR: Two-Step Protocol
RT-PCR: Two-Step Protocol We will provide both one-step and two-step protocols for RT-PCR. We recommend the twostep protocol for this class. In the one-step protocol, the components of RT and PCR are mixed
DNA Sequencing of the eta Gene Coding for Staphylococcal Exfoliative Toxin Serotype A
Journal of General Microbiology (1988), 134, 71 1-71 7. Printed in Great Britain 71 1 DNA Sequencing of the eta Gene Coding for Staphylococcal Exfoliative Toxin Serotype A By SUSUMU SAKURA, HTOSH SUZUK
Heraeus Sepatech, Kendro Laboratory Products GmbH, Berlin. Becton Dickinson,Heidelberg. Biozym, Hessisch Oldendorf. Eppendorf, Hamburg
13 4. MATERIALS 4.1 Laboratory apparatus Biofuge A Centrifuge 5804R FACScan Gel electrophoresis chamber GPR Centrifuge Heraeus CO-AUTO-ZERO Light Cycler Microscope Motopipet Neubauer Cell Chamber PCR cycler
Thermo Scientific Phusion Site-Directed Mutagenesis Kit #F-541
PRODUCT INFORMATION Thermo Scientific Phusion Site-Directed Mutagenesis Kit #F-541 Lot _ Store at -20 C Expiry Date _ www.thermoscientific.com/onebio CERTIFICATE OF ANALYSIS The Phusion Site-Directed Mutagenesis
inhibition of mitosis
The EMBO Journal vol.13 no.2 pp.425-434, 1994 cdt 1 is an essential target of the Cdc 1 O/Sct 1 transcription factor: requirement for DNA replication and inhibition of mitosis Johannes F.X.Hofmann and
N-terminal Regulatory Domains of Phosphodiesterases 1, 4, 5 and 10 examined with an Adenylyl Cyclase as a Reporter
N-terminal Regulatory Domains of Phosphodiesterases 1, 4, 5 and 10 examined with an Adenylyl Cyclase as a Reporter Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Eberhard Karls Universität
For information regarding shipping specifications, please refer to the following link: http://dna.macrogen.com/eng/help/orderg.jsp
Macrogen Sample Submission Guide Macrogen has served over 10 years in sequencing field using the cutting edge technology and delivering fast and reliable results. We use high throughput Applied Biosystems
Supporting Information
Supporting Information Wiley-VCH 2007 69451 Weinheim, Germany Evolving a Thermostable DNA polymerase that Accurately Amplifies Highly-Damaged Templates Christian Gloeckner, Katharina B. M. Sauter, and
Characterization of cdna clones of the family of trypsin/a-amylase inhibitors (CM-proteins) in barley {Hordeum vulgare L.)
Characterization of cdna clones of the family of trypsin/a-amylase inhibitors (CM-proteins) in barley {Hordeum vulgare L.) J. Paz-Ares, F. Ponz, P. Rodríguez-Palenzuela, A. Lázaro, C. Hernández-Lucas,
TA Cloning Kit. Version V 7 April 2004 25-0024. Catalog nos. K2000-01, K2000-40, K2020-20, K2020-40, K2030-01 K2030-40, K2040-01, K2040-40
TA Cloning Kit Version V 7 April 2004 25-0024 TA Cloning Kit Catalog nos. K2000-01, K2000-40, K2020-20, K2020-40, K2030-01 K2030-40, K2040-01, K2040-40 ii Table of Contents Table of Contents...iii Important
Introduction to Bioinformatics 3. DNA editing and contig assembly
Introduction to Bioinformatics 3. DNA editing and contig assembly Benjamin F. Matthews United States Department of Agriculture Soybean Genomics and Improvement Laboratory Beltsville, MD 20708 matthewb@ba.ars.usda.gov
Association of IGF1 and IGFBP3 polymorphisms with colorectal polyps and colorectal cancer risk
DOI 10.1007/s10552-009-9438-4 ORIGINAL PAPER Association of IGF1 and IGFBP3 polymorphisms with colorectal polyps and colorectal cancer risk Elisabeth Feik Æ Andreas Baierl Æ Barbara Hieger Æ Gerhard Führlinger
Taqman TCID50 for AAV Vector Infectious Titer Determination
Page 1 of 8 Purpose: To determine the concentration of infectious particles in an AAV vector sample. This process involves serial dilution of the vector in a TCID50 format and endpoint determination through
Nucleic Acid Chemistry Laboratory Automated DNA Sequencing Manual
Nucleic Acid Chemistry Laboratory Automated DNA Sequencing Manual To DNA Sequencers: This packet contains lots of information that will help you optimize your automated DNA sequencing samples. We have
Insulin Receptor Gene Mutations in Iranian Patients with Type II Diabetes Mellitus
Iranian Biomedical Journal 13 (3): 161-168 (July 2009) Insulin Receptor Gene Mutations in Iranian Patients with Type II Diabetes Mellitus Bahram Kazemi 1*, Negar Seyed 1, Elham Moslemi 2, Mojgan Bandehpour
Six Homeoproteins and a Iinc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype
Six Homeoproteins and a Iinc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype Iori Sakakibara 1,2,3, Marc Santolini 4, Arnaud Ferry 2,5, Vincent Hakim 4, Pascal Maire 1,2,3 * 1 INSERM U1016,
Distribution of the DNA transposon family, Pokey in the Daphnia pulex species complex
Eagle and Crease Mobile DNA (2016) 7:11 DOI 10.1186/s13100-016-0067-7 RESEARCH Open Access Distribution of the DNA transposon family, Pokey in the Daphnia pulex species complex Shannon H. C. Eagle and
Archimer http://archimer.ifremer.fr
Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site Fish
Mutation. Mutation provides raw material to evolution. Different kinds of mutations have different effects
Mutation Mutation provides raw material to evolution Different kinds of mutations have different effects Mutational Processes Point mutation single nucleotide changes coding changes (missense mutations)
Biopython Tutorial and Cookbook
Biopython Tutorial and Cookbook Jeff Chang, Brad Chapman, Iddo Friedberg, Thomas Hamelryck, Michiel de Hoon, Peter Cock Last Update September 2008 Contents 1 Introduction 5 1.1 What is Biopython?.........................................
Metabolic Engineering of Escherichia coli for Enhanced Production of Succinic Acid, Based on Genome Comparison and In Silico Gene Knockout Simulation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Dec. 2005, p. 7880 7887 Vol. 71, No. 12 0099-2240/05/$08.00 0 doi:10.1128/aem.71.12.7880 7887.2005 Copyright 2005, American Society for Microbiology. All Rights
Chlamydomonas adapted Green Fluorescent Protein (CrGFP)
Chlamydomonas adapted Green Fluorescent Protein (CrGFP) Plasmid pfcrgfp for fusion proteins Sequence of the CrGFP In the sequence below, all amino acids which have been altered from the wildtype GFP from
Interleukin-4 Receptor Signal Transduction: Involvement of P62
Interleukin-4 Receptor Signal Transduction: Involvement of P62 Den Naturwissenschaftlichen Fakultäten der Friedrich Alexander Universität Erlangen Nürnberg zur Erlangung des Doktorgrades vorgelegt von
Introduction to Bioinformatics (Master ChemoInformatique)
Introduction to Bioinformatics (Master ChemoInformatique) Roland Stote Institut de Génétique et de Biologie Moléculaire et Cellulaire Biocomputing Group 03.90.244.730 rstote@igbmc.fr Biological Function
Molecular detection of Babesia rossi and Hepatozoon sp. in African wild dogs (Lycaon pictus) in South Africa
Available online at www.sciencedirect.com Veterinary Parasitology 157 (2008) 123 127 Short communication Molecular detection of Babesia rossi and Hepatozoon sp. in African wild dogs (Lycaon pictus) in
http://hdl.handle.net/10197/2727
Provided by the author(s) and University College Dublin Library in accordance with publisher policies. Please cite the published version when available. Title Performance of DNA data embedding algorithms
Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer s disease is this type 3 diabetes?
Journal of Alzheimer s Disease 7 (2005) 63 80 63 IOS Press Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer s disease is this type 3 diabetes? Eric Steen,
Chapter 5. Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development
Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development This chapter has been adapted from: W.K. Smits*, C.C. Eschevins*, K.A. Susanna, S. Bron, O.P.
Bio 102 Practice Problems Recombinant DNA and Biotechnology
Bio 102 Practice Problems Recombinant DNA and Biotechnology Multiple choice: Unless otherwise directed, circle the one best answer: 1. Which of the following DNA sequences could be the recognition site
9. Materials. 9.1 Chemicals Acetic Acid (glacial) Materials. 8-Aminoguanosine Ammonium Hydroxide Ammonium Persulfate.
9. Materials 9.1 Chemicals Acetic Acid (glacial) Acetone Acetonitrile Agarose 8-Aminoguanosine Ammonium Hydroxide Ammonium Persulfate Ampicillin ATP Bromophenol Blue Butanol Chloroform CTP 7-Deaza-GTP
BD BaculoGold Baculovirus Expression System Innovative Solutions for Proteomics
BD BaculoGold Baculovirus Expression System Innovative Solutions for Proteomics Table of Contents Innovative Solutions for Proteomics...........................................................................
BacReady TM Multiplex PCR System
BacReady TM Multiplex PCR System Technical Manual No. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI Detailed Experimental
APOT - Assay. Protocol for HPV16 and 18. Amplification of Papilloma Virus Oncogene Transcripts HPV. E6 E7 E1 Zelluläre DNA poly(a)
E5 E2 E1 APOT - Assay Amplification of Papilloma Virus Oncogene Transcripts URR E6 E7 L1 HPV L2 E4 E6 E7 E1 Zelluläre DNA poly(a) Protocol for HPV16 and 18 Brief summary of the APOT assay Fig.1A shows
MultiSite Gateway Pro
MultiSite Gateway Pro Using Gateway Technology to simultaneously clone multiple DNA fragments Catalog nos. 12537-102, 12537-103, 12537-104, 12537-100 Version B 3 October 2006 25-0942 Design your MultiSite
Description: Molecular Biology Services and DNA Sequencing
Description: Molecular Biology s and DNA Sequencing DNA Sequencing s Single Pass Sequencing Sequence data only, for plasmids or PCR products Plasmid DNA or PCR products Plasmid DNA: 20 100 ng/μl PCR Product:
Universidade Estadual de Maringá
Universidade Estadual de Maringá Disciplina: Biologia Molecular Sequenciamento de ácidos nucléicos Profa. Dra. Maria Aparecida Fernandez Maxan e Gilbert - quebra química Berg, Gilbert and Sanger dideoxinucleotideos
ISTEP+: Biology I End-of-Course Assessment Released Items and Scoring Notes
ISTEP+: Biology I End-of-Course Assessment Released Items and Scoring Notes Page 1 of 22 Introduction Indiana students enrolled in Biology I participated in the ISTEP+: Biology I Graduation Examination
The Arabinosyltransferase EmbC Is Inhibited by Ethambutol in Mycobacterium tuberculosis
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Oct. 2009, p. 4138 4146 Vol. 53, No. 10 0066-4804/09/$08.00 0 doi:10.1128/aac.00162-09 Copyright 2009, American Society for Microbiology. All Rights Reserved. The
pentr Directional TOPO Cloning Kits
user guide pentr Directional TOPO Cloning Kits Five-minute, directional TOPO Cloning of blunt-end PCR products into an entry vector for the Gateway System Catalog numbers K2400-20, K2420-20, K2525-20,
Molecular Facts and Figures
Nucleic Acids Molecular Facts and Figures DNA/RNA bases: DNA and RNA are composed of four bases each. In DNA the four are Adenine (A), Thymidine (T), Cytosine (C), and Guanine (G). In RNA the four are
restriction enzymes 350 Home R. Ward: Spring 2001
restriction enzymes 350 Home Restriction Enzymes (endonucleases): molecular scissors that cut DNA Properties of widely used Type II restriction enzymes: recognize a single sequence of bases in dsdna, usually
Differentiation of Klebsiella pneumoniae and K. oxytoca by Multiplex Polymerase Chain Reaction
Differentiation of Klebsiella pneumoniae and K. oxytoca by Multiplex Polymerase Chain Reaction Yogesh Chander 1 M. A. Ramakrishnan 1 Naresh Jindal 1 Kevan Hanson 2 Sagar M. Goyal 1 1 Department of Veterinary
How To Clone Into Pcdna 3.1/V5-His
pcdna 3.1/V5-His A, B, and C Catalog no. V810-20 Rev. date: 09 November 2010 Manual part no. 28-0141 MAN0000645 User Manual ii Contents Contents and Storage... iv Methods... 1 Cloning into pcdna 3.1/V5-His
SEQUENCING SERVICES. McGill University and Génome Québec Innovation Centre JANUARY 21, 2016. Version 3.4
JANUARY 21, 2016 SEQUENCING SERVICES McGill University and Génome Québec Innovation Centre Sanger Sequencing User Guide Version 3.4 Copyright 2010 McGill University and Génome Québec Innovation Centre
PrimeSTAR HS DNA Polymerase
Cat. # R010A For Research Use PrimeSTAR HS DNA Polymerase Product Manual Table of Contents I. Description...3 II. III. IV. Components...3 Storage...3 Features...3 V. General Composition of PCR Reaction
Mutation of the SPSl-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation
Mutation of the SPSl-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation Helena Friesen/ Rayna Lunz/* Steven Doyle,^ and Jacqueline
Directed-Mutagenesis and Deletion Generated through an Improved Overlapping-Extension PCR Based Procedure
Research Article Directed-Mutagenesis and Deletion Generated through an Improved Overlapping-Extension PCR Based Procedure Wirojne Kanoksilapatham 1*, Juan M. González 2 and Frank T. Robb 3 1 Department
Complete Amino Acid Sequence and in vitro Expression of Rat NF-M, The Middle Molecular Weight Neurofilament Protein
The Journal of Neuroscience, August 1987, 7(8): 2590-2599 Complete Amino Acid Sequence and in vitro Expression of Rat NF-M, The Middle Molecular Weight Neurofilament Protein Eugene W. Napolitano, Steven
Protein Synthesis Simulation
Protein Synthesis Simulation Name(s) Date Period Benchmark: SC.912.L.16.5 as AA: Explain the basic processes of transcription and translation, and how they result in the expression of genes. (Assessed
2 Materials and Methods
2 Materials and Methods 2.1 Materials 2.1.1 Chemicals, reagents and consumable materials Chemicals were used from the companies Sigma (München), Roth (Karlsruhe), Merck (Darmstadt), Fluka (Neu-Ulm), Serva
Recombinant DNA Unit Exam
Recombinant DNA Unit Exam Question 1 Restriction enzymes are extensively used in molecular biology. Below are the recognition sites of two of these enzymes, BamHI and BclI. a) BamHI, cleaves after the