How To Use Chemistry In Bioinorganic Chemistry
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- Ethelbert Marsh
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1 Some techniques used in bioinorganic chemistry 1. Mass Spectrometry (not an exhaustive list) 2. UV-Vis Absorption Spectroscopy & Fluorescence Emission Spectroscopy 3. CD Spectroscopy 4. NMR Spectroscopy (including paramagnetic NMR with contact shifts) 5. NMR relaxation ation times to map the distance between een the paramagnetic ion and nmr nucleus 6. Extended X-ray absorption fine structure spectroscopy (EXAFS) 7. Single crystal X-ray diffraction 8. Electrochemistry with cyclic voltammetry 9. Fluorescence excited state life time measurements 10.EPR Spectroscopy 11.Mossbauer Spectroscopy 12.Protein isolation & purification 13.Gel electrophoresis (PAGE)
2 Mass spectrometry Electrospray Ionization Mass Spectrometry (ESI MS) Matrix Assisted Laser Desorption Ionization (MALDI)
3 Few examples based on absorption spectroscopy
4 Absorption Titrations
5 Emission spectroscopy By Intrinsic Methods: Presence of Trp, to some extent by Phe By Extrinsic Methods: Any other fluorescent species either directly or attached to a biomolecular probe Protein can also be modified by a fluorophore Ion concentrations & ph etc., can be measured even in side the cells Excited state life time measurements Based on Intrinsic OR extrinsic groups Either based on protein or based on the small molecule that interacts and/or binds or both
6 Few examples based on NMR spectroscopy Ad-OP(O) 2 OP(O) 2 OPO 3 Ad-OP(O) 2 OP(O) 2 CH 2 PO 3 1 H NMR of Co(II) - metallothionein
7 NMR: Dynamics & Paramagnetic relaxation Determination of internuclear distances in an enzyme bound Mn-organophosphate by relaxation time measurements
8 Single crystal X-ray diffraction nλ = 2dSinθ d = nλ/2sinθ
9 Extended X-ray Absorption Fine Structure Spectroscopy
10 Electrochemistry: Cyclic voltammetry
11 Electrochemical Titrations
12
13
14 EPR Spectroscopy: Few examples
15 Electron Paramagnetic Resonance Spectroscopy
16 Mossbauer Spectroscopy
17 Circular Dichroism Spectroscopy CD & Absorption: Full line: Deoxy Hb; Dashed line: Oxy Hb; Dotted line: met Hb
18 Species equilibria in solution
19 Microscopy Microscopy methods such as: Atomic Force Microscopy (AFM) Scanning Electron Microscopy (SEM) Transmission Electron Microscopy (TEM) Protein small molecular complexes --- their nano-structural features Protein aggregations --- induced by organic and inorganic species etc. Variety of properties such as conductivity & electrochemistry Protein & nucleic acid samples can be studied in solution it self Studies are also extended to nanoparticles based biomolecular systems
20 Protein Isolation
21 Gel Electrophoresis: Protein purity, Mwt & Protein small molecular binding
22 Books where you may find SOME relevant material S. No Name of the book 1 Bioinorganic Chemistry: a short course 2 Bioinorganic Chemistry- an inorganic perspective of life 3 Principles of Bioinorganic Chemistry Author(s) Rosette M. Roat-Malone Dimitris P. Kessissoglou Stephen J. Lippard, Jeremy Mark Berg Year of Publication Publisher 2007 John Wiley & sons, Inc Kluwer Academic Publishers 1994 University Science Books 4. Inorganic Biochemistry: An Introduction James A Cowan 1993 VCH 5 Bioinorganic Chemistry Eckhard Bill 1991 Springer-Verlag 6 Bioinorganic Chemistry Ivano Bertini 1994 University Science Books 7 8 Bioinorganic Chemistry: The Biological Chemistry of Transition Metals Physical methods in bioinorganic chemistry: spectroscopy and magnetism Michael Watkinson Ivano Bertini, 9 Handbook on metalloproteins Astrid Sigel, Helmut Sigel 10 Bioinorganic chemistry: transition metals in biology and their coordination chemistry 2009 John Wiley & Sons, Limited it Lawrence Que 2000 University Science Books Alfred Trautwein, Deutsche 1997 Forschungsgeme inschaft 2001 CRC Press Deutsche Forschungsgemeinschaft
23 Books where you may find SOME relevant material S. No Name of the book Author(s) Year of Publication Publisher From cells to atoms An 11 illustrated introduction to molecular biology A.R. Rees, M.J.E. Sternberg, & Sir David Philips Blackwell Scientific Publications
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