Curriculum Vitae Dr. Daniel Tietze

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1 Curriculum Vitae Dr. Daniel Tietze BUSINESS ADDRESS Technische Universität Darmstadt Eduard Zintl Institute for Inorganic and Physical Chemistry Alarich Weiss Str Darmstadt Germany Phone: Fax.: PERSONAL DATA Date of birth: Place of birth: Nationality: Family status: Profession: Specialized in: 1980 Pößneck, Germany German married, one child Chemist (Diploma) Physical Chemistry (PhD) EDUCATION AND ACADEMIC CAREER 10/ / / / /2005 PhD defense, summa cum laude PhD in physical chemistry, Friedrich Schiller University, Institute of Physical Chemistry, Jena, Germany, Prof. Dr. G. Buntkowsky (now Technische Universität Darmstadt) Title: Investigations on the Mechanism of Nickel Superoxide Dismutase Using Peptide Based Model Systems Diploma in chemistry, grade: excellent, Technical Chemistry and Environmental Chemistry, Friedrich Schiller University Jena, Germany, Prof. Dr. G. Kreisel, Dr. S. Meyer Title: Photooxidation von Citronellol in verschiedenen Reaktortypen ( Photo oxidation of citronellol in various reactor types ) 10/ /2005 Study of chemistry, Friedrich Schiller University Jena, Germany

2 PROFESSIONAL EXPERIENCE 08/2013 current 07/ / / /2012 Habilitand, Junior scientist & research group leader Eduard Zintl Institute for Inorganic and Physical Chemistry, Technische Universität Darmstadt, Prof. Dr. Gerd Buntkowsky Project: NMR as a tool to study structure and function of peptides and ion channels PostDoc, Department of Chemistry, Iowa State University, Ames, USA, Prof. Dr. M. Hong (now Massachusetts Institute of Technology MIT, Cambridge, MA, USA) Project: Conformation and dynamics of the influenza A and influenza B proton channel investigated by solid state NMR PostDoc, Eduard Zintl Institute for Inorganic and Physical Chemistry, Technische Universität Darmstadt, Prof. Dr. Gerd Buntkowsky SCHOLARSHIPS AND AWARDS Fonds der Chemischen Industrie research grant, project: NMR structure analysis, in silico ligand design, theoretical binding studies of novel Gq/11 inhibitors. DAAD travel grant, 56th Rocky Mountain Conference on Magnetic Resonance July 13 th 17 th 2014, Copper Mountain, Colorado, USA Travel grant, 3rd winter school on Biomolecular Solid State NMR, Jan. 6 th 11 th 2013, Stowe, Vermont, USA Travel grant, SMARTER 2 workshop, May 23 rd 27 th 2011, Aveiro, Portugal INVITED LECTURES 1. invited talk: 12 th German Peptide Symposium, March , Darmstadt, Germany 2. invited talk: Pharmazeutisches Montagskolloqium der Pharmazie, November 2013, Institute of Pharmaceutical Biology, Rheinische Friedrich Wilhelms Universität Bonn, Bonn, Germany 3. invited talk: June 2013, Chemistry Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA, USA 2

3 PARTICIPATION AT INTERNATIONAL CONFERENCES 1. talk: D. Tietze Exploring enzyme function through model peptides: Conformation and Dynamics studied by solid state NMR, 12 th German Peptide Symposium, March , Darmstadt, Germany 2. poster: J. K. Williams, D. Tietze, J. Wang, Y. Wu, W. F. DeGrado, M. Hong SSNMR Studies of the Conformation, Dynamics and Small Molecule Interactions of Wild Type and S31N Mutant Influenza M2 Proton Channels, 56th Rocky Mountain Conference on Magnetic Resonance July , Copper Mountain, CO, USA 3. poster: D. Tietze, S. Voigt, M. Tischler, G. Buntkowsky Revealing the position of the substrate in NiSOD: a model study, SMARTER 2 workshop, May , Aveiro, Portugal 4. poster: D. Tietze, S. Voigt, M. Tischler, D. Imhof, H. Breitzke, G. Buntkowsky Investigations on the Catalytic Mechanism of Nickel Superoxide Dismutase using solid state NMR EUROMAR, July , Florence, Italy 5. talk: D. Tietze Investigations on the Catalytic Mechanism of Nickel Superoxide Dismutase using liquid and solid state NMR techniques Ampere NMR School, June , Wierzba, Poland 6. talk: D. Tietze Investigations on the Catalytic Mechanism of Nickel Superoxide Dismutase Ampere NMR School, June , Zakopane, Poland 7. talk: D. Tietze Investigations on the Catalytic Mechanism of Nickel Superoxide Dismutase AK Treffen Festkörper NMR, March , Heidelberg, Germany 8. poster: D. Tietze, D. Imhof, H. Breitzke, G. Buntkowsky Mechanistic Investigations on NiSOD Biomimetics Using Solid State NMR, Ampere NMR School, June , Poznań/ Wierzba, Poland COLLABORATIONS (MAJOR) Prof. Mei Hong, PhD, Department of Chemistry, MIT Massachusetts Institute of Technology, Cambridge, MA, USA Prof. Dr. Evi Kostenis, Institute of Biological Pharmacy, Rheinische Friedrich Wilhelms Universität Bonn Prof. Dr. Gerd Buntkowsky, Eduard Zintl Institute for Physical and Inorganic Chemistry, Technische Universität Darmstadt Prof. Dr. Doreen Mollenhauer, Theoretical Chemistry, Justus Liebig University, Gießen Prof. Dr. Diana Imhof, Institute of Pharmacy, Rheinische Friedrich Wilhelms Universität Bonn Prof. Dr. Gabriele M. König, Institute of Biological Pharmacy, Rheinische Friedrich Wilhelms Universität Bonn 3

4 Prof. Dr. Thomas Prisner, Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Frankfurt, bio molecular EPR group Prof. Dr. Stefan H. Heinemann, Institute of Biochemistry and Biophysics, University of Jena, electrophysiology group Prof. M. Ashley Spies, PhD, Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa, Iowa City, IA, USA Prof. Dr. Harald Kolmar, Institute of Biochemistry, Technische Universität Darmstadt Prof. Dr. Gerhard Thiel, Department of Biology, Technische Universität Darmstadt Prof. Dr. Bodo Laube, Department of Biology, Technische Universität Darmstadt Dr. Indra Schröder, Department of Biology, Technische Universität Darmstadt Dr. A. A. Tietze, Institute of Biochemistry, Technische Universität Darmstadt METHODS NMR SPECTROSCOPY Solution NMR: structure determination of peptides and proteins, protein ligand interactions, analysis of dynamics and conformation of peptides and proteins (standard techniques such as NOESY, ROESY plus various spectral editing techniques) Solid state NMR: structure determination of membrane proteins in lipid environment, proteinligand interactions, dynamics and conformation analysis of proteins and peptides, NMR crystallography (standard solid state NMR techniques such as CP MAS, REDOR, HETCOR, DARR, 19 F CODEX, DIPSHIFT) Software: assignment/ data analysis: CCPN analysis, TopSpin 3.2, structure calculation: YASARA structure, XPLORE NIH, Talos CHEMISTRY AND PEPTIDE CHEMISTRY: Chemistry: Schlenk techniques, organo metallic synthesis, protecting group chemistry for amino acid derivates Peptide chemistry: Solution phase synthesis, solid phase synthesis, ligation strategies, manual and automated syntheses Targets compounds: amino acid derivatives, short, medium sized and long peptides, miniproteins, membrane proteins, peptidomimetics Peptide analytics: analytical and semipreparative HPLC (RP, IEC), TLC 4

5 BIOCHEMISTRY/PROTEIN BIOCHEMISTRY: Protein protein and protein ligand interactions: spectroscopic methods (e.g. NMR, UV/Vis); Enzymology: activity measurements, catalytic mechanism of enzymes, enzyme inhibitors, UV Vis and fluorescent enzymatic assays (Stopped Flow, HPLC) Mass spectrometry of peptides and proteins: MALDI TOF/TOF MS THEORETICAL CHEMISTRY: Protein dynamics/ motion analysis: Molecular dynamics simulation (NAMD, YASARA structure), targeted molecular dynamics simulation, normal mode analysis Protein ligand interactions: molecular docking (Autodock, Autodock VINA, Rosetta docking) library screening (YASARA) 5

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