1 n Introduction to Next-eneration Sequencing echnology Deciphering DN sequences is essential for virtually all branches of biological research. With the advent of capillary electrophoresis (E)-based Sanger sequencing, scientists gained the ability to elucidate genetic information from any given biological system. his technology has become widely adopted in laboratories around the world, yet has always been hampered by inherent limitations in throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential information they need for their course of study. o overcome these barriers, an entirely new technology was required Next-eneration Sequencing (NS), a fundamentally different approach to sequencing that triggered numerous ground-breaking discoveries and ignited a revolution in genomic science.
2 Welcome to Next-eneration Sequencing he five years since the introduction of NS technology have seen a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome, and epigenome of any species. his ability has catalyzed a number of important breakthroughs, advancing scientific fields from human disease research to agriculture and evolutionary science. In principle, the concept behind NS technology is similar to E the bases of a small fragment of DN are sequentially identified from signals emitted as each fragment is re-synthesized from a DN template strand. NS extends this process across millions of reactions in a massively parallel fashion, rather than being limited to a single or a few DN fragments. his advance enables rapid sequencing of large stretches of DN base pairs spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. o illustrate how this process works, consider a single genomic DN (gdn) sample. he gdn is first fragmented into a library of small segments that can be uniformly and accurately sequenced in millions of parallel reactions. he newly identified strings of bases, called reads, are then reassembled using a known reference genome as a scaffold (resequencing), or in the absence of a reference genome (de novo sequencing). he full set of aligned reads reveals the entire sequence of each chromosome in the gdn sample (Figure 1). Figure 1: onceptual Overview of Whole-enome Resequencing DN Fragments Sequencing Reads Reference enome B D gdn Parallel Sequencing lignment Sequence. Extracted gdn B. gdn is fragmented into a library of small segments that are each sequenced in parallel.. Individual sequence reads are reassembled by aligning to a reference genome D. he whole-genome sequence is derived from the consensus of aligned reads.
3 High-hroughput Science NS data output has increased at a rate that outpaces Moore s law, more than doubling each year since it was invented. In 2007, a single sequencing run could produce a maximum of around one gigabase (b) of data. By 2011, that rate has nearly reached a terabase (b) of data in a single sequencing run nearly a 1000 increase in four years. With the ability to rapidly generate large volumes of sequencing data, NS enables researchers to move quickly from an idea to full data sets in a matter of hours or days. Researchers can now sequence more than five human genomes in a single run, producing data in roughly one week, for a reagent cost of less than $5,000 per genome. By comparison, the first human genome required roughly 10 years to sequence using E technology and an additional three years to finish the analysis. he completed project was published in 2003, just a few years before NS was invented, and came with a price tag nearing 3 billion USD. While the latest high-throughput sequencing instruments are capable of massive data output, NS technology is highly scalable. he same underlying chemistry can be used for lower output volumes for targeted studies or smaller genomes. his scalability gives researchers the flexibility to design studies that best suit the needs of their particular research. For sequencing small bacterial/viral genomes or targeted regions like exomes, a researcher can choose to use a lower output instrument and process a smaller number of samples per run, or can opt to process a large number of samples by multiplexing on a high-throughput instrument. Multiplexing enables large sample numbers to be simultaneously sequenced during a single experiment (Figure 2). o accomplish this, individual barcode sequences are added to each sample so they can be differentiated during the data analysis. Figure 2: onceptual Overview of Sample Multiplexing DN Fragments Sequencing Reads Reference enome Sample 1 Barcode Sample 2 Barcode B D Sample 1 Sample 2. wo representative DN fragments from two unique samples, each attached to a specific barcode sequence that identifies the sample from which it originated. B. Libraries for each sample are pooled and sequenced in parallel. Each new read contains both the fragment sequence and its sampleidentifying barcode.. Barcode sequences are used to de-multiplex, or differentiate reads from each sample. D. Each set of reads is aligned to the reference sequence.
4 With multiplexing, NS dramatically reduces the time to data for multi-sample studies. Processing hundreds of amplicons using E technology generally requires several weeks or months. he same number of samples can now be sequenced in a matter of hours and fully analyzed within two days using NS. With highly automated, easy-to-use protocols, researchers can go from experiment to data to publication faster and easier than ever before (able 1). able 1: comparison of Illumina NS and E-Based Sanger Sequencing echnology Starting Material Samples per Run Run ime* Read Length Number of Reads Output per Run pplications E-based Sanger Method Illumina MiSeq System Illumina HiSeq System 1 3 µg ng Nextera kit µg ruseq kit 50 ng Nextera kit µg ruseq kit 1 lane flow cell Single or dual 16- lane flow cell 0.5 hrs 550 bp kb DN sequencing, resequencing, microsatellite analysis, SNP genotyping 3 hrs 900 bp kb 3.4 million (single reads) 27 hrs bp** days bp 3 billion (single reads) 1 b Up to 600 b DN sequencing, gene regulation analysis, quantitative and qualitative sequencing-based transcriptome analysis, SNP discovery and structural variation analysis, cytogenetic analysis, DN-protein interaction analysis (hip-seq), sequencing-based methylation analysis, small RN discovery and analysis, de novo, metagenomics, metatranscriptomics * E-based run time does not include 4-hour sequencing reaction on the thermocycler prior to loading. NS sequencing and base detection occurs concurrently during the run. Base pairs with quality scores of 20 (Q20). > 90% of base pairs have quality scores of 30 (Q30). ** > 75% of base paris have quality scores of 30 (Q30). > 80% of base pairs have quality scores of 30 (Q30). unable Resolution NS provides a high degree of flexibility for the level of resolution required for a given experiment. sequencing run can be tailored to produce more or less data, zoom in with high resolution on particular regions of the genome, or provide a more expansive view with lower resolution. o adjust the level of resolution, a researcher can tune the coverage generated for a particular type of experiment. he term coverage generally refers to the average number of sequencing reads that align to each base within the sample DN. For example, a whole genome sequenced at 30 coverage means that, on average, each base in the genome was covered by 30 sequencing reads. he ability to easily tune the level of coverage and resolution offers a number of experimental design advantages. For instance, in cancer research, somatic mutations may only exist within a small proportion of cells in a given tissue sample. Using mixed-cell samples, the region of DN harboring the mutation must be sequenced at very high levels of coverage, upwards of 1000, to detect these low frequency mutations within the cell population. While this type of analysis is possible with E technology, there is an additive cost incurred with each additional read, so experiments requiring high read depths can become prohibitively expensive, especially when scaling the process across a number of samples. On the other side of the coverage spectrum, a researcher would likely choose a much lower coverage level for an application like genome-wide variant discovery. In this case, it makes more sense to sequence at lower resolution, but process larger sample numbers to achieve greater statistical power within a given population of interest.
5 Unlimited Dynamic Range he digital nature of NS supports an unlimited dynamic range, providing very high sensitivity for quantifying applications, such as gene expression analysis. With NS, researchers can quantify RN activity at much higher resolution than traditional microarray-based methods, important for capturing subtle gene expression changes associated with biological processes. Where microarrays measure continuous signal intensities, with a detection range limited by noise at the low end and signal saturation at the high end, NS quantifies discrete, digital sequencing read counts. By increasing or decreasing the number of sequencing reads, researchers can tune the sensitivity of the experiment to accommodate different study objectives. Universal Biology ool he powerful and flexible nature of NS has permeated many areas of study, becoming firmly entrenched as an indispensable and universal tool for biological research. With the ability to analyze the genetic architecture of any biological entity, the scientific community has used this technology platform to develop a broad range of applications that have transformed study designs, surpassing boundaries, and unlocking information never before imaginable. Imagine what NS can do for your research. See the new sequencer that can deliver it to your lab. Visit FOR RESERH USE ONLY 2011 Illumina, Inc. ll rights reserved. Illumina, illuminadx, Beadrray, BeadXpress, cbot, SPro, DSL, DesignStudio, Eco, IIx, enetic Energy, enome nalyzer, enomestudio, oldenate, HiScan, HiSeq, Infinium, iselect, MiSeq, Nextera, Sentrix, Solexa, ruseq, Veraode, the pumpkin orange color, and the enetic Energy streaming bases design are trademarks or registered trademarks of Illumina, Inc. ll other brands and names contained herein are the property of their respective owners. Pub No urrent as of 29 ugust 2011
6 n Introduction to Next-eneration Sequencing echnology Part II: n overview of DN Sequencing pplications Diverse pplications Next-generation sequencing (NS) platforms enable a wide variety of applications, allowing researchers to ask virtually any question of the genome, transcriptome, and epigenome of any organism. Sequencing applications are largely dictated by the way sequencing libraries are prepared and the way the data is analyzed, with the actual sequencing stage remaining fundamentally unchanged. here are a number of standard library preparation kits that offer protocols for sequencing whole genomes, mrn, targeted regions such as whole exomes, custom-selected regions, protein-binding regions, and more. o address specific research objectives, many researchers have developed novel protocols to isolate specific regions of the genome associated with a given biological function. Sample preparation protocols for NS are generally more rapid and straightforward than those for capillary electrophoresis (E)-based Sanger sequencing (able 1). With NS, researchers can start directly from a gdn or cdn library. he DN fragments are then ligated to platform-specific oligonucleotide adapters needed to perform the sequencing biochemistry, requiring as little as 90 minutes to complete (Figure 1). In contrast, E-based Sanger sequencing requires genomic DN to be fragmented first and cloned into either bacterial artificial chromosomes (Bs) or yeast artificial chromosomes (Ys). hen, each B/Y must be further subcloned into a sequencing vector and transformed into the appropriate microbial host. emplate DN is then purified from individual colonies or plaques prior to sequencing. his process can take days or even weeks to complete, depending on the size of the genome. H 3
7 able 1: Sample Preparation for Whole-enome Sequencing at a lance E-based Sanger Sequencing Library preparation more involved each sample must contain a single template, requiring purification from single bacterial, yeast colonies, or phage plaques Next-eneration Sequencing Library preparation more streamlined sample can consist of a population of DN molecules that do not require clonal purification omplete within days to weeks, depending upon the size of the genome being sequenced omplete within hours, regardless of genome size Figure 1: NS Library Preparation dapters DN Sequencing-Ready Library NS library preparation starts directly from fragmented genomic DN. Platform-specific oligonucleotide adapters, needed to perform the sequencing biochemistry and index samples, are ligated to each end of the fragments to yield the sequencing-ready library. Data nalysis lgorithms long with sample preparation, data analysis is an important factor to consider for sequencing applications. range of data analysis algorithms are available that perform specific tasks related to a given application. Some applications, such as de novo sequencing, require specialized assembly of sequencing reads. Other applications, such as RN-Seq, require algorithms that quantify read counts to provide information about gene expression levels. number of algorithms exist to address the needs of each application. While some of these are commercially available from software vendors, many are freely available open-source algorithms from academic institutions. Illumina collaborates closely with commercial and academic software developers to create an ecosystem of data analysis tools that address the needs of various research objectives 1.
8 Whole-enome Sequencing Until recently, sequencing an entire genome was a major endeavor. Even for fairly compact viral genomes with overlapping genes and few to no repetitive regions, whole-genome sequencing using E-based Sanger technology requires a significant commitment of time and resources. For example, de novo whole-genome sequencing of vaccinia virus a large and complex DN virus (~200 kb) using E-based methods would involve roughly 4,000 sequencing reactions (assuming 10 coverage and 500 bp read lengths), each conducted in separate tubes or wells. Using NS technology, the same sequencing project, including library preparation, can be completed in just a few days with one sequencing run at 30 coverage or greater. Sequencing Small enomes he ability of NS platforms to produce a large volume of data in a short period of time makes it a powerful tool for whole-genome sequencing in a laboratory environment. While NS is commonly associated with sequencing large genomes, especially human genomes, the scalability of the technology makes it just as useful for small viral or bacterial genomes. his was demonstrated during the recent E. coli (EHE) outbreak in Europe, which prompted a rapid scientific response. Using the latest NS systems, researchers were able to quickly generate a high-quality whole-genome sequence of the bacterial strain 2, enabling them to better understand the genetic mutations conferring the increased virulence. One challenge associated with sequencing small genomes is the lack of reference genomes available for most species. his means that whole-genome sequencing must often be done de novo, where the reads are assembled without aligning to a reference sequence. he coverage quality of a de novo sequencing data set depends upon the quality of the contigs, or continuous sequences generated by aligning overlapping sequencing reads. he size and continuity of the contigs will affect the number of gaps present in the data. problem for de novo sequencing is that the short read lengths generated by NS can lead to a higher number of gaps, regions where no reads align, resulting in greater fragmentation and smaller contigs poorer data quality. his is especially true for regions of the genome containing repetitive sequence elements. o overcome this challenge, some NS platforms offer paired-end (PE) sequencing protocols (Figure 2), where both ends of a DN fragment are sequenced, as opposed to single-read sequencing where only one end is sequenced. Paired-end reads result in superior alignment across regions containing repetitive sequences and produce longer contigs for de novo sequencing by filling gaps in the consensus sequence, resulting in complete overall coverage. Figure 2. Paired-End Sequencing and lignment Paired-End Reads lignment to the Reference Sequence Read 1 Reference Read 2 Repeats Paired-end sequencing enables both ends of the DN fragment to be sequenced. Because the distance between each paired read is known, alignment algorithms can use this information to map the reads over repetitive regions more precisely. his results in much better alignment of the reads, especially across difficult-to-sequence, repetitive regions of the genome.
9 Figure 3. De Novo ssembly with Mate Pairs Short-Insert Paired End Reads De Novo ssembly Read 1 Read 2 Long-Insert Paired End Reads (Mate Pair) Read 1 Read 2 Using a combination of short and long insert sizes with paired-end sequencing results in maximal coverage of the genome for de novo assembly. Because larger inserts can pair reads across greater distances, they provide a better ability to read through highly repetitive sequences and regions where large structural rearrangements have occurred. Shorter inserts sequenced at higher depths can fill in gaps missed by larger inserts sequenced at lower depths. hus a diverse library of short and long inserts results in better de novo assembly, leading to fewer gaps, larger contigs, and greater accuracy of the final consensus sequence. nother important factor in generating high quality de novo sequences is the diversity of insert (DN fragment) sizes in the library. Using longer inserts provides the highest fragment diversity relative to starting input material, yielding more uniform sequencing coverage. When long inserts are prepared for pair-end sequencing, a mate pair library is generated. hese can include insert sizes ranging from 2 to 5 kb, optimal for de novo assembly applications, including both genome scaffold generation and genome finishing. In general, libraries with larger insert sizes will result in less fragmented assemblies and larger contigs. ombining short-insert paired-end and long-insert mate pair sequencing is the most powerful approach for maximal coverage across the genome (Figure 3). he combination of insert sizes enables detection of the widest range of structural variant types and is essential for accurately identifying more complex rearrangements, which results in a higher quality assembly. he short-insert reads sequenced at higher depths can fill in gaps not covered by the long inserts, which are often sequenced at lower read depths. In parallel with NS technological improvements, many algorithmic advances have been made in de novo sequence assemblers for short-read data. Researchers can perform high-quality de novo assembly using NS reads and publicly available short-read assemblers. In many instances, existing computer resources in the laboratory are enough to perform de novo assemblies. he E. coli genome can be assembled in as little as 15 minutes using a 32-bit Windows desktop computer with 32 B of RM. argeted Sequencing With targeted sequencing, only a subset of genes or defined regions in a genome are sequenced, allowing researchers to focus time, expenses, and data storage on the regions of the genome in which they are most interested. his approach is typically used to sequence many individuals to discover, screen, or validate genetic variation within a population. he ability to pool samples and obtain high sequence coverage during a single run allows NS to identify rarer variants that are missed, or too expensive to identify, using E-based sequencing approaches. here are two different methods for making libraries for targeted sequencing and resequencing projects target enrichment and amplicon generation.
10 mplicon Sequencing mplicon sequencing allows researchers to sequence small, selected regions of the genome spanning hundreds of base pairs. he latest NS amplicon library preparation kits allow researchers to perform rapid in-solution amplification of custom-targeted regions from genomic DN. Using this approach, thousands of amplicons spanning multiple samples can be simultaneously prepared and indexed in a matter of hours. With the ability to process numerous amplicons and samples on a single run, NS is much more cost-effective than E-based Sanger sequencing technology, which does not scale with the number of regions and samples required in complex study designs. NS enables researchers to simultaneously analyze all genomic content of interest in a single experiment, at fraction of the time and cost. able 2: argeted Sequencing Sample Prep at a lance E-based Sanger Sequencing Library preparation more involved each sample must contain a single template, either from a single PR purified from single bacterial colonies Suitable for sequencing amplicons and clone checking omplete within days to weeks, depending upon the size of the genome being sequenced Next-eneration Sequencing Library preparation more streamlined each sample can be a population and does not require clonal purification Suitable for sequencing amplicons and clone checking omplete within hours his highly targeted NS approach enables a wide range of applications for discovering, validating, and screening genetic variants for various study objectives. For example, sequencing amplicons at a high depth of coverage can identify common and rare sequence variations. With sufficient coverage depth, deep sequencing can characterize rare sequence variants in a population, with minor allele frequencies below 1%. his application is particularly useful for the discovery of rare somatic mutations in complex samples (e.g., cancerous tumors mixed with germline DN). Hence, amplicon sequencing is well-suited for clinical environments, where researchers are examining a limited number of disease-related variants. nother common amplicon application is sequencing the bacterial 16S rrn gene across a number of species, a widely used method for studying phylogeny and taxonomy, particularly in diverse metagenomic samples 3. his method has been used to evaluate bacterial diversity in a number of environments, allowing researchers to characterize microbiomes from samples that are otherwise difficult or impossible to study. n overwhelming majority of the world s micorganisms have evaded cultivation, but sequencing-based metagenomic analyses are finally making it possible to investigate their ecological, medical, and industrial relevance 4. arget Enrichment arget enrichment is similar to amplicon capture in that selected regions or genes are enriched in the library from genomic DN. he difference is that target enrichment allows for larger DN insert sizes and enables a greater amount of total DN to be sequenced per sample. his ability lets researchers expand the information they can garner from each sample. For example, rather than sequencing a few hundred exons, they can sequence the entire exome to call functional single nucleotide polymorphisms (SNPs) for an individual. Sequencing human exomes from many individuals enables rare disease-associated alleles to be identified within a population.
11 Figure 4: arget Enrichment In target enrichment, selected regions are captured from the library by probes bound to magnetic beads. Once the unbound DN in the library is washed away, the capture DN is eluted to provide an enriched library. Numerous kits exist for target enrichment. Researchers can choose to either design custom probes or use standard kits for widely used applications, such as exome enrichment. With custom design, researchers can target regions of the genome relevant to their research interests. his is ideal for examining specific genes in pathways, or as a follow-up analysis to genome-wide association studies (WS). Segments of the genome identified in a WS as harboring causative variants can be enriched and sequenced to identify additional, possibly rarer, variants in the associated region. he steps involved in making a NS library for target enrichment are virtually identical to the steps involved in library generation for whole-genome sequencing, with the addition of a target enrichment step (Figure 4). enomic DN is isolated, fragmented, purified to ensure uniform and appropriate fragment size, and end-ligated to oligonucleotide adapters specific to the NS platform. he complexity of a sequencing library can then be reduced by enriching for specific regions of interest, such as exons or specific genes, by immobilizing DN on a substrate such as magnetic beads. fter binding the library and washing away unbound DN, a simple elution step allows recovery of the library, which can then be amplified by PR in preparation for sequencing.
12 From Innovation to Publication he advent of NS has enabled researchers to study biological systems at a level never before possible. s the technology has evolved, an increasing number of innovative sample preparation methods and data analysis algorithms have enabled a broad range of scientific applications. Researchers are making fascinating discoveries in a number of biological fields, unlocking answers never before possible. s a result, there has been an explosion in the number of scientific publications. Illumina sequencing alone has resulted in over 1,900 peer-reviewed publications in just five years, a feat unsurpassed by any previous life science technology. Selected recent examples are listed below. Whole-enome Sequencing Srivatsan, et al. (2008) High-precision, whole-genome sequencing of laboratory strains facilitates genetic studies. PLoS enet 4: e Rasmussen M, et al. (2010) ncient human genome sequence of an extinct Palaeo-Eskimo. Nature 463: Li R, et al. (2010) he sequence and de novo assembly of the giant panda genome. Nature 463: Pelak K, et al. (2010) he characterization of twenty sequenced human genomes. PLoS enet 6:e argeted Resequencing Ram JL, Karim S, Sendler ED, Kato (2011) Strategy for microbiome analysis using 16S rrn gene sequence analysis on the Illumina sequencing platform. Syst Biol Reprod Med. 57(3): McEllistrem M.. (2009) enetic diversity of the pneumococcal capsule: implications for molecular-based serotyping. Future Microbiol 4: Lo YMD, hiu RWK. (2009) Next-generation sequencing of plasma/serum DN: an emerging research and molecular diagnostic tool. lin. hem 55: Robinson PN (2010) Whole-exome sequencing for finding de novo mutations in sporadic mental retardation. enome Biol 11:144. raya L, Fowler DM (2011) Deep mutational scanning: assessing protein function on a massive scale. rends Biotechnol. doi: /j.tibtech References Bomar L, Maltz M, olston S, and raf J. (2011) Directed culturing of microorganisms using metatranscriptomics. mbio. 2:e FOR RESERH USE ONLY 2011 Illumina, Inc. ll rights reserved. Illumina, illuminadx, Beadrray, BeadXpress, cbot, SPro, DSL, DesignStudio, Eco, IIx, enetic Energy, enome nalyzer, enomestudio, oldenate, HiScan, HiSeq, Infinium, iselect, MiSeq, Nextera, Sentrix, Solexa, ruseq, Veraode, the pumpkin orange color, and the enetic Energy streaming bases design are trademarks or registered trademarks of Illumina, Inc. ll other brands and names contained herein are the property of their respective owners. Pub No urrent as of 23 September 2011
n Introduction to Next-eneration Sequencing echnology Part II: n overview of DN Sequencing pplications Diverse pplications Next-generation sequencing (NS) platforms enable a wide variety of applications,
An Introduction to Next-eneration Sequencing Technology Deciphering DNA sequences is essential for virtually all branches of biological research. With the advent of capillary electrophoresis (CE)-based
Go where the biology takes you. Genome Analyzer IIx Genome Analyzer IIe Go where the biology takes you. To published results faster With proven scalability To the forefront of discovery To limitless applications
n Introduction to Illumina Next-eneration Sequencing Technology for Microbiologists Deciphering DN sequences is essential for virtually all branches of biological research. apillary electrophoresis (E)-based
Data Sheet: Targeted Resequencing TruSeq Custom Amplicon v1.5 A new and improved amplicon sequencing solution for interrogating custom regions of interest. Highlights Figure 1: TruSeq Custom Amplicon Workflow
Sequencing power for every scale. Systems for every application, for every lab. Proven sequencing technology. Accelerate your research. Achieve your next breakthrough. What started as novel Illumina chemistry,
i Technical Note: Reproductive Health Rapid Aneuploidy and CNV Detection in Single Cells using the MiSeq System Comparison between data generated from single cells using 24sure array-based screening and
SEQUENCING From Sample to Sequence-Ready ACCESS ARRAY SYSTEM HIGH-QUALITY LIBRARIES, NOT ONCE, BUT EVERY TIME The highest-quality amplicons more sensitive, accurate, and specific Full support for all major
The Power of Next-Generation Sequencing in Your Hands On the Path towards Diagnostics The GS Junior System The Power of Next-Generation Sequencing on Your Benchtop Proven technology: Uses the same long
Next Generation Sequencing Technology and applications 10/1/2015 Jeroen Van Houdt - Genomics Core - KU Leuven - UZ Leuven 1 Landmarks in DNA sequencing 1953 Discovery of DNA double helix structure 1977
Data Processing of Nextera Mate Pair Reads on Illumina Sequencing Platforms Introduction Mate pair sequencing enables the generation of libraries with insert sizes in the range of several kilobases (Kb).
IFU022 v140202 Research Use Only Instructions For Use Part III Data Analysis for Ion Torrent Sequencing MANUFACTURER: Multiplicom N.V. Galileilaan 18 2845 Niel Belgium Revision date: August 21, 2014 Page
PN 100-9879 A1 TECHNICAL NOTE Single-Cell Whole Genome Sequencing on the C1 System: a Performance Evaluation Introduction Cancer is a dynamic evolutionary process of which intratumor genetic and phenotypic
History of DNA Sequencing & Current Applications Christopher McLeod President & CEO, 454 Life Sciences, A Roche Company IMPORTANT NOTICE Intended Use Unless explicitly stated otherwise, all Roche Applied
Buyer s Guide: Next-Generation Sequencing Systems Covering All the Bases: What to Consider Before Making Your NGS System Purchase. This is an exciting time for biological research. Meaningful discovery
STGAAC STGAACT GTGCACT GTGAACT STGAAC STGAACT GTGCACT GTGAACT STGAAC STGAAC GTGCAC GTGAAC Wouter Coppieters Head of the genomics core facility GIGA center, University of Liège Bioruptor NGS: Unbiased DNA
Buyer s Guide: Automated Library Preparation for Next-Generation Sequencing What to consider as you evaluate options for automating library preparation. Yes, success can be automated. Next-generation sequencing
De Novo Assembly Using Illumina Reads High quality de novo sequence assembly using Illumina Genome Analyzer reads is possible today using publicly available short-read assemblers. Here we summarize the
APPLICATION NOTE Amplicon Sequencing Ion Torrent Amplicon Sequencing Introduction The ability to sequence a genome or a portion of a genome has enabled researchers to begin to understand how the genetic
Precise TM Whitepaper Introduction LIMITATIONS OF EXISTING RNA-SEQ METHODS Correctly designed gene expression studies require large numbers of samples, accurate results and low analysis costs. Analysis
Genetic Analysis Phenotype analysis: biological-biochemical analysis Behaviour under specific environmental conditions Behaviour of specific genetic configurations Behaviour of progeny in crosses - Genotype
Genetic engineering: humans Gene replacement therapy or gene therapy Many technical and ethical issues implications for gene pool for germ-line gene therapy what traits constitute disease rather than just
Overview of Next Generation Sequencing platform technologies Dr. Bernd Timmermann Next Generation Sequencing Core Facility Max Planck Institute for Molecular Genetics Berlin, Germany Outline 1. Technologies
Targeted TARGETED Sequencing sequencing solutions Accurate, scalable, fast Sequencing for every lab, every budget, every application Ion Torrent semiconductor sequencing Ion Torrent technology has pioneered
Introduction to next-generation sequencing data David Simpson Centre for Experimental Medicine Queens University Belfast http://www.qub.ac.uk/research-centres/cem/ Outline History of DNA sequencing NGS
Technical Note: Illumina Systems and Software Consistent Assay Performance Across Universal Arrays and Scanners There are multiple Universal Array and scanner options for running Illumina DASL and GoldenGate
GenomeStudio Data Analysis Software Illumina has created a comprehensive suite of data analysis tools to support a wide range of genetic analysis assays. This single software package provides data visualization
GenomeStudio Analysis Software Illumina has created a comprehensive suite of data analysis tools to support a wide range of genetic analysis assays. This single software package provides data visualization
Core Facility Genomics versatile genome or transcriptome analyses based on quantifiable highthroughput data ascertainment 1 Topics Collaboration with Harald Binder and Clemens Kreutz Project: Microarray
The Digital Biology TM Company Targeted DNA Sequencing Solutions It Pays To Be On Target Proven Platform RainDance TM Technologies is pioneering digital biology and powering ground-breaking human genetics
Fishing for variants in the deep end of the gene pool: OGT s custom bait designs Jolyon Holdstock, Simon Hughes and Daniel Swan Abstract Oxford Gene Technology (OGT) has extensive expertise in probe design
An Introduction to Next-Generation Sequencing for in vitro Fertilization www.illumina.com/ivfprimer Table of Contents Part I. Welcome to Next-Generation Sequencing 3 NGS for in vitro Fertilization 3 Part
Human Tissue RNA-Seq Data from the Illumina HiSeq 2000 System Gary Schroth & the Gene Expression Applications Group Research & Development 2009 Illumina, Inc. All rights reserved. Illumina, illuminadx,
Next generation DNA sequencing technologies theory & prac-ce Outline Next- Genera-on sequencing (NGS) technologies overview NGS applica-ons NGS workflow: data collec-on and processing the exome sequencing
DNA Sequence Analysis Two general kinds of analysis Screen for one of a set of known sequences Determine the sequence even if it is novel Screening for a known sequence usually involves an oligonucleotide
Genotyping by sequencing and data analysis Ross Whetten North Carolina State University Stein (2010) Genome Biology 11:207 More New Technology on the Horizon Genotyping By Sequencing Timeline 2007 Complexity
Molecular typing of VTEC: from PFGE to NGS-based phylogeny Valeria Michelacci 10th Annual Workshop of the National Reference Laboratories for E. coli in the EU Rome, November 5 th 2015 Molecular typing
PLNT2530 Unit 6e DNA Sequencing Unless otherwise cited or referenced, all content of this presenataion is licensed under the Creative Commons License Attribution Share-Alike 2.5 Canada 1 High-throughput
Advances in RainDance Sequence Enrichment Technology and Applications in Cancer Research March 17, 2011 Rendez-Vous Séquençage Presentation Overview Core Technology Review Sequence Enrichment Application
Contents Decode File Client User Guide Contents... 1 Notice... 2 Getting Started... 3 Workflow Overview... 3 Preliminary Steps... 3 Using the Decode File Client in Access by Account Mode... 3 Using the
MiSeq: Imaging and Page Welcome Navigation Presenter Introduction MiSeq Sequencing Workflow Narration Welcome to MiSeq: Imaging and. This course takes 35 minutes to complete. Click Next to continue. Please
Contig N50 (kb) APPLICATION NOTE Ion PGM small genome sequencing The Ion PGM System, with 400-base read length chemistry, enables routine high-quality de novo assembly of small genomes Key findings: MiSeq
Introduction to transcriptome analysis using High Throughput Sequencing technologies (HTS) A typical RNA Seq experiment Library construction Protocol variations Fragmentation methods RNA: nebulization,
July 7th 2009 DNA sequencing Overview Sequencing technologies Sequencing strategies Sample preparation Sequencing instruments at MPI EVA 2 x 5 x ABI 3730/3730xl 454 FLX Titanium Illumina Genome Analyzer
TECHNOLOGY OVERVIEW Complete Genomics Sequencing Introduction With advances in nanotechnology, high-throughput instruments, and large-scale computing, it has become possible to sequence a complete human
1 of 8 11/7/2004 11:00 AM National Center for Biotechnology Information About NCBI NCBI at a Glance A Science Primer Human Genome Resources Model Organisms Guide Outreach and Education Databases and Tools
ONE WORKFLOW. ONE PARTNER. ACTIONABLE INSIGHTS. Sample to Insight (NGS) Nucleic Acid More actionable data and insights in one go. More confidence at one glance. A small step for you, a giant leap for your
How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss
Leading Genomics Diagnostic harma Discove Collab Shanghai Cambridge, MA Reykjavik Global leadership for using the genome to create better medicine WuXi NextCODE provides a uniquely proven and integrated
Microarray Solutions for Genome-Wide Genetic Analysis Custom & Catalog Microarrays High Quality Data CALL OR LEARN MORE AT: 1-800-227-9770 www.agilent.com/genomics For Research Use Only. Not for use in
2013 Laboratory Accreditation Program Audioconferences and Webinars Implementing Next Generation Sequencing (NGS) as a Clinical Tool in the Laboratory Nazneen Aziz, PhD Director, Molecular Medicine Transformation
Product Bulletin Sequencing Software SeqScape Software Version 2.5 Comprehensive Analysis Solution for Resequencing Applications Comprehensive reference sequence handling Helps interpret the role of each
An Overview of DNA Sequencing Prokaryotic DNA Plasmid http://en.wikipedia.org/wiki/image:prokaryote_cell_diagram.svg Eukaryotic DNA http://en.wikipedia.org/wiki/image:plant_cell_structure_svg.svg DNA Structure
Focusing on results not data comprehensive data analysis for targeted next generation sequencing Daniel Swan, Jolyon Holdstock, Angela Matchan, Richard Stark, John Shovelton, Duarte Mohla and Simon Hughes
Single Nucleotide Polymorphism (SNP) Calling from Next-Gen Sequencing (NGS) data for Bacterial Phylogenetics Taj Azarian, MPH Doctoral Student Department of Epidemiology College of Medicine and College
A Primer of Genome Science THIRD EDITION GREG GIBSON-SPENCER V. MUSE North Carolina State University Sinauer Associates, Inc. Publishers Sunderland, Massachusetts USA Contents Preface xi 1 Genome Projects:
DNA sequencing the beginnings Ghent University (Fiers et al) pioneers sequencing first complete gene (1972) first complete genome (1976) Next Generation Sequencing Fred Sanger develops dideoxy sequencing
DN Sequencing Ben Langmead Department of omputer Science You are free to use these slides. If you do, please sign the guestbook (www.langmead-lab.org/teaching-materials), or email me (email@example.com)
Introduction to NGS data analysis Jeroen F. J. Laros Leiden Genome Technology Center Department of Human Genetics Center for Human and Clinical Genetics Sequencing Illumina platforms Characteristics: High
Introduction To Real Time Quantitative PCR (qpcr) SABiosciences, A QIAGEN Company www.sabiosciences.com The Seminar Topics The advantages of qpcr versus conventional PCR Work flow & applications Factors
Presented by Yvette Conley, PhD What we will cover during this webcast: Briefly discuss the approaches introduced in the paper: Genome Sequencing Genome Wide Association Studies Epigenomics Gene Expression
Scientific Working Group on DNA Analysis Methods Validation Guidelines for DNA Analysis Methods Table of Contents Introduction.2 1. Definitions. 2 2. General Considerations 3 3. Developmental Validation..5
How is genome sequencing done? Using 454 Sequencing on the Genome Sequencer FLX System, DNA from a genome is converted into sequence data through four primary steps: Step One DNA sample preparation; Step
Poster Note As presented at AGBT 215, Marco Island, FL KAPA HYPER PLUS: A single-tube NGS library prep workflow integrating enzymatic fragmentation results in high yields and low sequencing bias. Authors
Product Bulletin SNP Genotyping SNPbrowser Software v3.5 A Free Software Tool for the Knowledge-Driven Selection of SNP Genotyping Assays Easily visualize SNPs integrated with a physical map, linkage disequilibrium
Assuring the Quality of Next-Generation Sequencing in Clinical Laboratory Practice Next-generation Sequencing: Standardization of Clinical Testing (Nex-StoCT) Workgroup Principles and Guidelines Supplementary
Name Period The AP Biology exam has reached into this chapter for essay questions on a regular basis over the past 15 years. Student responses show that biotechnology is a difficult topic. This chapter
An Introduction to Next-eneration Sequencing for Cell Biologists www.illumina.com/nstech Table of Contents Welcome to Next-eneration Sequencing 3 Why Next-eneration Sequencing? 3 High-Throughput Science
Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue Application Guide... 2 I. Description... 2 II. Product Components... 2 III. Materials to be Supplied
Name Date lass Master 19 Basic oncepts Recombinant DN Use with hapter, Section.2 Formation of Recombinant DN ut leavage Splicing opyright lencoe/mcraw-hill, a division of he Mcraw-Hill ompanies, Inc. Bacterial
restriction enzymes 350 Home Restriction Enzymes (endonucleases): molecular scissors that cut DNA Properties of widely used Type II restriction enzymes: recognize a single sequence of bases in dsdna, usually
UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 10-K ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December
BRCA1 / 2 testing by massive sequencing highlights, shadows or pitfalls? Giovanni Luca Scaglione, PhD ------------------------ Laboratory of Clinical Molecular Diagnostics and Personalized Medicine, Institute
Multiplex your most important genetic assays on one platform GenomeLab GeXP Genetic Analysis System Blood Banking Capillary Electrophoresis Centrifugation Flow Cytometry Genomics Lab Automation Lab Tools
Introduction to NGS Technologies Ignacio Medina firstname.lastname@example.org Head of Computational Biology Lab HPC Service, University of Cambridge, UK EMBL-EBI Scientific collaborator Genome Campus, Hinxton, Cambridge,
Description: Molecular Biology s and DNA Sequencing DNA Sequencing s Single Pass Sequencing Sequence data only, for plasmids or PCR products Plasmid DNA or PCR products Plasmid DNA: 20 100 ng/μl PCR Product:
NGS data analysis Bernardo J. Clavijo 1 A brief history of DNA sequencing 1953 double helix structure, Watson & Crick! 1977 rapid DNA sequencing, Sanger! 1977 first full (5k) genome bacteriophage Phi X!
RETRIEVING SEQUENCE INFORMATION Nucleotide sequence databases Database search Sequence alignment and comparison Biological sequence databases Originally just a storage place for sequences. Currently the
Ankyrin 3 Genetic Association Studies of Bipolar Disorders Wade Berrettini, MD, PhD The Karl E. Rickels Professor of Psychiatry and Director of the Center for Neurobiology and Behavior, Department of Psychiatry
Next Generation Sequencing Catalog 2014-2015 Introduction Bioo Scientific Bioo Scientific is a global life science company headquartered in Austin, TX, committed to providing innovative products and superior
White Paper Oncology Insights Enabled by Knowledge Base-Guided Panel Design and the Seamless Workflow of the GeneReader NGS System Abstract: This paper describes QIAGEN s philosophy and process for developing
Next Generation Sequencing DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection Over the past three years, massively
Using Illumina BaseSpace Apps to Analyze RNA Sequencing Data The Illumina TopHat Alignment and Cufflinks Assembly and Differential Expression apps make RNA data analysis accessible to any user, regardless
MODULE 2 Objective 2.1 Lesson E GFP Transformation Genetic Manipulations Course Advanced Biotechnology Unit DNA Technology Essential Question How is foreign DNA genes taken up by organisms and expressed?
New Technologies for Sensitive, Low-Input RNA-Seq Clontech Laboratories, Inc. Outline Introduction Single-Cell-Capable mrna-seq Using SMART Technology SMARTer Ultra Low RNA Kit for the Fluidigm C 1 System
Systematic discovery of regulatory motifs in human promoters and 30 UTRs by comparison of several mammals Xiaohui Xie 1, Jun Lu 1, E. J. Kulbokas 1, Todd R. Golub 1, Vamsi Mootha 1, Kerstin Lindblad-Toh