Shimadzu s Total Support for Food Safety

Analytical and Testing Instruments for Food Safety Shimadzu s Total Support for Food Safety C10G-E020B

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Shimadzu's Total Support for Food Safety Shimadzu provides total support for food safety, whether it is farm to fork or bait to plate. As a leading manufacturer of a wide range of analytical instruments, Shimadzu undertakes development of new instruments and technologies, and provides comprehensive service support in order to keep up with changing market demands. In addition to offering analytical instruments and testing machines, Shimadzu provides total support that includes the provision of information for the food safety community, training at workshops and seminars, and instrument maintenance management. Contents P.4 P.7 P.8 P.9 P.10 P.11 P.12 P.13 P.14 P.15 P.16 Analysis of Residual Pesticides Analysis of Mycotoxins Identification and Observation of Microorganisms Analysis of Veterinary Pharmaceuticals Analysis of Food Additives Analysis of Toxic Metals Inspection and Analysis of Foreign Matter Food Processing Analysis of Flavor & Texture Prevention of Defects in Packaging List of Food Testing Application by Instrument

Analysis of Residual Pesticides Food knows no borders. A pesticide used in one country may be banned in another country, or even banned around the world because of health risks. This has resulted in increased testing of pesticides in agricultural products as there are over 1,000 known pesticides. Shimadzu offers a wide range of instruments for pesticide testing, from GC and LC with specific detectors to single and triple quadrupole mass spectrometers. MRM Analysis of Residual Pesticides in Food (GC-MS/MS) Analysis of 360 pesticides in apple extract in 10 minutes using a GCMS-TQ8040 with Smart MRM. Triple Quadrupole Mass Spectrometer GCMS-TQ8040 Analysis of 360 pesticides by GC-MS/MS in less than 10 minutes with Smart MRM Finally, a triple quadrupole GC-MS/MS Smart enough for everyday use in your laboratory. The Shimadzu GCMS-TQ8040 is the first triple quadrupole with Smart Productivity for high-efficiency sample throughput, Smart Operation for quick and easy method development, and Smart Performance for low detection limits and Scan/MRM. These three smart technologies contribute to Smart MRM and provide the most accurate, cost-effective, and easy-to-use triple quadrupole GCMS you have ever imagined. Smart productivity 400+ compounds in one run Smart MRM software Twin Line MS Kit Smart operation MRM optimization tool Smart Database series Automatic Method Creation Smart performance Scan/MRM acquisition mode High-speed scanning Automatic Adjustment of Retention Time (AART). So Smart it almost runs itself! Options Smart Pesticide Database The Smart Pesticide Database includes optimized transitions and collision energies, CAS registry numbers, and Retention Indices (RI) for hundreds of pesticides. Select from pre-registered compounds in one database, or add you own optimized transitions. Select the compounds to be analyzed, and Smart MRM builds the MRM or Scan/MRM acquisition method from the Smart Database with a push of a button. Pesticide Quick-DB The software package contains Scan/MRM and Scan/SIM acquisition methods, and pre-stored calibration curves for semi-quantitative results for 480 pesticides within one hour of installation. Single Quadrupole Mass Spectrometer GCMS-QP2010 Ultra High sensitivity The high-performance ion source improves and lowers the minimum limits of detection and quantitation for measurements of trace components. A reduced absolute sample injection volume prevents contamination of the insert. Simultaneous Scan/SIM measurements During high-sensitivity SIM measurements of trace components, Scan measurements are performed on unknown components missed by SIM. Retention time correction Significantly reduces the workload for multicomponent simultaneous analysis. 4

Liquid Chromatograph Mass Spectrometer (LC-MS/MS) LC-MS/MS MRM (Multiple Reaction Monitoring) analysis allows for rapid and sensitive detection of pesticides in complex matrices. An example of simultaneous multi-residue multi-class pesticide analysis is shown below. Triple Quadrupole Mass Spectrometer LCMS-8050 x10 5 1.5 Intensity 1.0 0.5 0 0.25 0.5 0.75 1.00 1.25 1.5 1.75 min Simultaneous analysis of 226 pesticides (10ng/mL) within two minutes using LC-MS/MS Ultra-high speed Ultra-fast polarity switching at 5 msec. Ultra-fast scanning at 30,000 u/sec. Ultra-fast MRM transition speeds at 555 MRMs/sec. High sensitivity Newly improved ion optics and collision cell provide higher MRM and Scan sensitivity, which expands the potential range of LC-MS/MS applications. User -friendly Minimize instrument downtime with easy maintenance. A single-vendor solution of LC and MS provides seamless operation. In addition, it provides for simplistic MRM optimization. Liquid Chromatograph Mass Spectrometer (LC-MS) Single quadrupole LC-MS enables rapid and sensitive quantitative analysis of a wide variety of analytes. An example of vitamin analysis is shown below. Single Quadrupole Mass Spectrometer LCMS-2020 ( 1,000,000) 4.5 2 Pyridoxine 4.0 3.5 3.0 2.5 2.0 1 Thiamin 3 Nicotinic acid 4 Nicotinamide 5 Pantothenic acid (+):TIC (+):265.10 (+):170.20 (+):124.20(2.90) (+):123.20(1.12) (+):220.20(1.92) (+):678.60(5.56) (+):377.20(2.95) (-):440.10(50.00) Rapid polarity switching (15 msec) allows detection of both positively and negatively charged species. Excellent linearity (R2 > 0.99) and 1-50 part-per-billion quantitation limits were obtained. 1.5 1.0 6 Cyanocobalamin 7 Riboflavin 8 Folic acid 0.5 0.0 0.0 2.5 5.0 7.5 10.0 12.5 min Chromatograms of 8 Water-Soluble Vitamins Shimadzu s Total Support for Food Safety Analytical and Testing Instruments for Food Safety 5

Analysis of Residual Pesticides Analysis of Residual Pesticides (GC) Capillary GC can be utilized for the analysis of residual pesticides in foods. Using a selective detector such as FPD, FTD or ECD offers highly sensitive analysis of trace pesticides. dichlorvos methamidophos acephate ethoprophos thiometon salithion terbufos diazinon cadusafos phorate etrimfos iprobenfos dichlofenthion cyanophos chlorpyrifos-methyl + dimethoate tolclofos-methyl pirimiphos-methyl chlorpyrifos (E)-dimethylvinphos formothion fenthion malathion fenitrothion (MEP) (E)-chlorfenvinphos (Z)-dimethylvinphos isophenphos + quinalphos fosthiazate phenthoate (Z)-chlorfenvinphos propaphos fosthiazate 2 + prothiofos profenofos + methidathion butamifos Chromatograms of normal meat sample and pesticides-spiked meat sample ethion + sulprofos edifenphos fensulfothion cyanofenphos EPN pyridaphenthion phosmet Pesticide-spiked meat dumpling sample (0.2 µ g/g equivalent) Normal meat dumpling sample phosalone pyraclofos Liquid Chromatograph (LC) GC or GC-MS techniques may not be suitable for analysis of residual pesticides that thermally decompose or have a highly polar chemical structure. In contrast, an HPLC equipped with a photodiode array detector can acquire reliable chromatograms with spectral information for building libraries to compare compounds. The Shimadzu carbamate analysis system incorporates derivatization technologies to achieve high-sensitivity, selective analysis of N-methyl carbamate pesticides. 60 50 40 30 20 mv Oxamyl Methomyl Methiocarb sulfoxide Methiocarb sulfone Aldicarb Bendiocarb Carbaryl Ethiofencarb Fenobucarb Methiocarb Gas Chromatograph GC-2010 Plus 10 0 0.0 5.0 10.0 15.0 20.0 25.0 min Chromatogram of a Standard Mixture of 10 N-Methylcarbamate Pesticides High-Performance Liquid Chromatograph Prominence Series Carbamates Pesticides Analysis System High-sensitivity detection The FPD (Flame Photometric Detector) provides powerful detection of phosphorous and sulfur-based pesticides. For example, the minimum detectable quantities of phosphorus and sulfur are 55 fgp/s and 3 fgs/s, respectively. Higher analytical efficiency and productivity With the addition of a new cooling fan and superior air flow optimization, the oven cooling speed has dramatically improved (450 C -> 50 C cooling time: 3.4 minutes). A self-diagnostic function prevents failure by confirming proper operation and periodic diagnostics prevent unexpected downtime. Multiple detectors in a single GC Three detectors can be simultaneously mounted in the GC-2010 Plus: ECD, effective for chlorinated and pyrethroid-based pesticides; FPD, effective for sulfur- and phosphorous-based pesticides; and FTD, effective for nitrogen- and phosphorous-based pesticides. Rapid injection An injection time of 10 seconds for a 10 µl sample enhances analysis efficiency. High sensitivity and linearity over a broad range Upgraded optical and electrical systems reduce noise for lower detection limits and ensure simultaneous analysis of major components and trace components. Low sample carryover Platinum coating minimizes the adsorption of sample components on to the needle surface. 6

Analysis of Mycotoxins LCMS-8050 Simultaneous Analysis of Mycotoxins (LC-MS/MS) LC-MS/MS is effective for analysis of mycotoxins in complex matrices. Shown is an example of simultaneous analysis of 45 mycotoxins by LC-MS/MS. All compounds, including positive and negative ions, were separated and detected with high sensitivity within 9 minutes. For analysis of mycotoxins with high sensitivity, eliminating carryover is indispensable. Modified rinse methods using the SIL-30AC autosampler can eliminate carryover of fumonisin. 12000000 11500000 11000000 10500000 10000000 9500000 9000000 8500000 8000000 7500000 7000000 6500000 6000000 5500000 5000000 4500000 4000000 3500000 3000000 2500000 2000000 1500000 1000000 500000 0-500000 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 min Chromatogram of 45 mycotoxins at 50 ppb (2 ppb for aflatoxins and ochratoxines) SIL-30ACMP Multi-Plate Autosampler Carryover evaluation of fumonisins In a batch analysis of multiple samples, compounds with greatly differing polarities are frequently analyzed together. In cases such as this, sufficient rinsing between samples to reduce carryover may not be achieved using one type of rinse solution. To combat this, the SIL-30ACMP design incorporates sample-contacting materials to which components are less likely to adsorb, and a multi-solvent internal and external needle and loop rinsing protocol designed to reduce carryover to less than 0.0015%. Analysis of Aflatoxins (UHPLC) The analysis of aflatoxins (B1, B2, G1 and G2) is very important from a food safety perspective due to concerns about adverse effects on human health. The Nexera Ultra High Performance Liquid Chromatograph achieves high-resolution analysis of aflatoxins in less than four minutes. The RF-20Axs high-sensitivity fluorescence detector allows direct analysis of aflatoxins at trace levels (ppt) without any derivatization. Ultra High Performance Liquid Chromatograph Nexera X2 with RF-20Axs B1, G1 : 20ng/L B2, G2 : 5ng/L Analysis of aflatoxins in wheat flour Shimadzu s Total Support for Food Safety Analytical and Testing Instruments for Food Safety 7

Identification and Observation of Microorganisms In the event of food poisoning, identification of the causative factor is extremely important in determining the path of infection, prescribing treatment and preventing recurrence. Shimadzu s idplus microorganism identification platform provides rapid identification of microorganisms in food. Identification of Microorganisms (MALDI-TOF-MS) In order to prevent pathogenic germs from contaminating food sources, it is essential to identify them quickly and reliably in order to allow for proper control measures. A microorganism identification system using the AXIMA MALDI-TOF MS enables direct measurement of cultured microorganisms and identification within two minutes. AXIMA idplus Microorganism Identification System % Int. Salmonella enterica subsp. Enterica 3640.52 3829.04 4054.41 4434.57 4308.68 4365.39 5209.27 5033.17 5524.87 5369.13 6043.95 6352.79 6091.59 6671.91 6885.93 7230.02 7158.16 7590.49 7658.66 7886.39 8348.89 9520.27 3890.60 4307.86 5255.03 6253.14 7156.44 7867.55 8364.74 8871.39 9059.12 9235.80 9733.78 10220.70 11027.94 11424.07 4000 6000 8000 10000 12000 14000 m/z Comparing mass spectra of different bacteria What is Microorganism Identification by MALDI-TOF MS? Species-specific mass spectral patterns from microorganisms can be obtained by direct measurement of microorganisms using MALDI-TOF MS. Identification of microorganisms can be performed by matching these collected spectra to the database. This is a fast, easy and reliable method for identification of microorganisms. 10950.18 Staphylococcus aureus subsp. Aureus 12397.15 11922.76 12295.04 12706.04 13225.69 13698.41 13762.64 14067.89 Pseudomonas aeruginosa 13645.06 14991.82 Escherichia coli Bacillus subtilis Quick and easy analysis Three steps from sample preparation to identification. The microorganism identification process is finished within two minutes after starting the analysis. Highly accurate identification Microorganism identification by MALDI-TOF MS is a well-established technique with a low number of false positives. Applicable for a wide range of microorganisms Capable of classifying/identifying gram-positive and gram-negative bacteria, yeasts, fungi and spores. idplus identifies mircoorganisms by family, genus, species, and subspecies. Database customization Easily register new microorganisms in the database, which allows rapid development of a high-quality database. Low operational cost Analysis can be performed for pennies per sample compared to expensive traditional techniques. Expanded research capabilities idplus is not limited to microorganism identification. Reflectron mode and MS/MS functionalities enable a true research platform. 8

Analysis of Veterinary Pharmaceuticals Veterinary pharmaceuticals are used in the diagnosis, cure, mitigation, treatment, and prevention of diseases in animals as well as in the promotion of growth. However, there is increasing concern about these drugs entering the food chain and their effects on the human body. These drugs are categorized in many classes, such as anabolic hormones, anthelmintics, antibiotics, beta-agonists, coccidiostats, corticosteroids, nonsteroidal anti-inflammatory drugs (NSAIDs), and sedatives. Below are a few examples of veterinary pharmaceutical analyses. Analysis of Beta-agonist (LC-MS/MS) Beta-agonists can be added to animal feed to work as growth promoters due to their ability to increase muscle mass and decrease fat deposition. However, due to their adverse effects on human health, these compounds have been banned for growth-promoting purposes in farm animals by regulatory agencies worldwide. Analysis of Anabolic Steroids (On-line SPE-LC-MS/MS) The use of natural and synthetic hormones to increase the weight of meat-producing animals is prohibited by regulatory agencies worldwide in order to protect consumers from the harmful effects of digesting hormone residues and their metabolites. Compliance with this legislation is monitored by the screening of a large number of animals every year. Cimaterol Terbutaline Salbutamol Zilpaterol Cimbuterol Hydroxymethylclenbuterol Clenproperol Ractopamine Clenbuterol Tulobuterol Bromobuterol Mabuterol Isoxsuprine Clenpenterol Mapenterol A: Water 0.2mM ammonium fluoride B: Methanol 0.2mM ammonium fluoride 7 1 4 2 8 5 3 6 SRM chromatograms of 15 beta-agonists extracted from bovine liver (0.5 x screening target concentration). All compounds were eluted within 3.7 minutes. Analysis of eight target steroids in less than 4.5 minutes: Peak #1 β-boldenone, #2 α-trenbolone, #3 Dienestrol, #4 Testosterone, #5 α-boldenone, #6 Hydroxystanozolol, #7 methyltestosterone, #8 Progesterone Analysis of Malachite Green and Leucomalachite Green in Fish (LC-MS) Malachite green is an organic dye used as an antimicrobial medication to treat white-spot disease and tail rot in aquarium fish. Its use is not authorized for farmed fish in some countries. As malachite green is metabolized to leucomalachite green in the human body, the simultaneous analysis of these two components is required. Below is an example of the LC-MS analysis of malachite green and leucomalachite green. Malachite Green Analysis of Chloramphenicol in Honey (LC-MS) Chloramphenicol is effective against many microorganisms. However, due to its serious side effects in humans, it is only used against potentially fatal infectious diseases. Chloramphenicol has not only been detected in fish and meat, but cases are occasionally reported of the detection of chloramphenicol in honey and royal jelly. ( 1,000) Inten.(X10,000) 321 8.50 8.0 7.0 m/z 321 [M-H]- 8.25 6.0 Leucomalachite Green 8.00 7.75 5.0 4.0 3.0 359 m/z 357 [M+Cl]- 7.50 2.0 7.25 6.0 7.0 8.0 9.0 min 1.0 367 384 0.0 275.0 300.0 325.0 350.0 375.0 m/z SIM Chromatograms of Malachite Green and Leucomalachite Green Chromatogram of honey samples unspiked and spiked with chloramphenicol MS spectrum from LC-MS analysis of chloramphenicol Shimadzu s Total Support for Food Safety Analytical and Testing Instruments for Food Safety 9

Analysis of Food Additives A wide variety of additives are used in foods such as preservatives, flavor enhancers, coloring agents and antioxidants. Strict monitoring of food stuffs is essential to detect the use of prohibited chemicals. Each country assigns a unique number or code of approved chemicals for that country. Shimadzu offers a wide range of products for detecting the prohibited chemicals, whether they were added intentionally or through contamination. Analysis of Dicyandiamide and Melamine in Milk Powders (LC-MS/MS) Melamine was found to be used as a protein-rich adulterant in pet food in 2007, and then in infant formula in 2008 in China. The outbreak of the melamine scandal that killed many dogs and cats as well as led to the deaths of six infants and illness of many others caused panic and great concerns worldwide. Recently, low levels of dicyandiamide (DCD) 7.5 5.0 2.5 Area (x10,000) DCD (85.1>68.1) R2 = 0.997 0.0 0.0 2.5 5.0 7.5 Conc. Area (x100,000) 3.5 Melamine (127.1>85.1) 3.0 R2 = 0.999 2.5 2.0 1.5 1.0 0.5 Calibration curves of DCD and melamine 0.0 0.0 2.5 5.0 7.5 Conc. residues were found in milk products from New Zealand. Instead of being added directly as an adulterant, the trace DCD found in milk products was explained to be relating to the grass contaminated by DCD. Dicyandiamide has been used to promote the growth of pastures for cows grazing. (x100) 4.5 (x1,000) 6.0 DCD 4.0 Melamine 5.0 (85.1>68.1) (127.1>85.1) 3.5 4.0 3.0 2.5 3.0 2.0 2.0 1.5 1.0 1.0 0.5 0.0 0.0 2.00 2.25 2.50 2.75 min 5.5 6.0 6.5 min Overlapping of six MRM peaks of 0.5 ng/ml DCD and melamine in milk powder matrix Analysis of Preservatives in Food (UV) Below are examples of the analysis of food samples spiked with preservatives. 0.7 8µg Analysis of Sudan Dyes in Curry Powder (LC) Sudan dyes are oil-soluble synthetic dyes used in industrial products. Their use is not authorized in food products in some countries. This is an example of LC analysis of Sudan dyes in curry powder. Abs. 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.5µg 0.4µg 0.3µg Sample 450 500 0.2µg 0.1µg 550 600 650 nm Abs. 0.6 0.5 0.4 0.3 0.2 0.1 6µg 4µg 2µg 250 300 350 nm mau 20 15 10 5 1 3 4 2 5 6 7 8 Curry powder +Sudan dyes Peaks 1 Sudan Orange G-a 2 Sudan Orange G-b 3 Sudan Red G 4 Sudan I 5 Sudan II 6 Sudan III 7 Sudan Red 7B 8 Sudan IV Sodium Nitrite in Meat Sorbic Acid in a Processed White Fish Product Curry powder 0 0.0 2.5 5.0 7.5 10.0 12.5 15.0 min UV-VIS Spectrophotometer UV-1800 Sudan Dyes in Curry Powder High resolution 1 nm resolution provides for high data reliability. Enhanced security Functions can be restricted according to user level. Instrument validation Automatic/semi-automatic validation of wavelength accuracy and wavelength reproducibility. 10

Analysis of Toxic Metals Toxic metals (arsenic, lead, cadmium, etc.) in foods must be controlled down to low concentration levels. Shimadzu atomic absorption spectrophotometers and ICP emission spectrometers offer highly sensitive analysis of trace elements in foods. Shimadzu s product lineup also includes X-ray fluorescence spectrometers that can perform elemental analysis without sample pretreatment. These instruments are ideal for screening analysis and emergency analysis. Analysis of Cd and Pb in Food Additives (AA) Analysis of cadmium (Cd) and lead (Pb) in α-cyclodextrin (cyclic oligosaccharide), a substance used in functional foods, pharmaceuticals, cosmetics, etc. The analysis was conducted by the AA-7000 atomic absorption spectrophotometer. Atomic Absorption Spectrophotometer AA-7000 Abs Cd STD 1 µg/l Abs Pb STD 10 µg/l Sample Sample w/spike w/spike Sample Sample w/o Spike w/o Spike 0 3sec 0 3sec Peak profiles of Cd and Pb Measurement results of Cd and Pb in α-cyclodextrin Element Cd Measured value <0.003 µg/g Spike and recovery rate 105 % Pb <0.07 µg/g 99 % Measures from trace levels to high concentrations Automatic switching between flame and furnace measurements. A single instrument handles analysis from ppb to ppm levels. Sophisticated automation Automation functions enhance analysis efficiency. For instance, automatic searching sets the optimal gas flow rate and burner height for flame measurements. Also enables automatic dilution and re-measurement for over ranged samples compared to the calibration curve. The autosampler allows automatic addition of spikes, matrix matching, and dilutions (300x) with true mixing capability. Advanced safety technology World's first AA with vibration sensor to automatically extinguish the flame in case of an earthquake. Automatic gas-leak checks and safety functions prevent misuse of the wrong burner head. Analysis of Trace Element Contamination in Fish Sample (ICP) Fish Protein Certified Reference Material for Trace Metals (DORM-4) was used as fish sample. The Shimadzu ICPE-9800 Series can provide a rapid method to simultaneously analyze trace elements in fish. The results show excellent correlation with the certified reference material. Simultaneous multi-element quantitative analysis Multiple elements can be analyzed simultaneously. High sensitivity and wide dynamic range Simultaneous analysis of trace components and major components Low running costs Shimadzu's unique mini torch halves argon gas consumption. Cd 214.438nm Standards Cr 205.552nm Standards Samples Peak profiles of Cd and Cr Samples ICP Emission Spectrometer ICPE-9800 Series Element As Cd Cr Cu Fe Ni Pb Se Zn Quantitative results of fish CRM DORM-4 Measured Value (1) (mg/kg) 7.07 0.312 1.74 15.5 3.17 1.17 0.392 3.36 50.3 Fish CRM DORM-4 Measured Value (2) (mg/kg) 6.83 0.312 1.74 15.6 3.18 1.18 0.439 4.46 51.9 Certified Value (mg/kg) 6.80 ± 0.64 0.306 ± 0.015 1.87 ± 0.16 15.9 ± 0.9 341 ± 27 1.36 ± 0.22 0.416 ± 0.053 3.56 ± 0.34 52.2 ± 3.2 Fish Protein Certified Reference Material for Trace Metals (DORM-4) was used as fish sample. Shimadzu s Total Support for Food Safety Analytical and Testing Instruments for Food Safety 11

Inspection and Analysis of Foreign Matter Foreign matter can become mixed with food products during the production process for a variety of reasons. The discovery and identification of foreign matter and ascertaining its source are essential for maintaining food safety. Shimadzu s non-destructive testing and analytical technologies can be used to detect foreign matter that is not externally visible and analyze for the elemental or molecular components. Analysis of Inorganic Foreign Matter (EDX) An energy-dispersive X-ray fluorescence spectrometer (EDXRF) permits the easy and rapid identification of inorganic foreign matter adhering to, or mixed into, food products. Below is an example of the qualitative analysis of a metal fragment on chocolate. Energy Dispersive X-ray fluorescence Spectrometer EDX-7000/8000 Quantitative analysis results Fe Cr Ni Mo Cu 70.06% 19.18% 9.77% 0.51% 0.48% Large sample chamber Accepts samples of up W300 x D275 x H100 mm. Easy operation Automates complex set-up operations. Even novices can easily conduct accurate measurements. Metal foreign matter on chocolate Analysis of Organic Foreign Matter (FTIR) IRTracer-100 FTIR Spectrophotometer The Fourier transform infrared (FTIR) spectrophotometer measures the organic foreign matter spectrum and compares it with library data to identify the foreign matter. The example below shows the analysis of contamination on a frozen pizza. Some of the foreign substance was scraped off the frozen pizza, and the infrared spectrum was measured using a transmission infrared microscope. The spectrum of this foreign object was similar to that of a fluororesin. 1 Abs 0.5 contaminant High sensitivity High S/N ratio above 60,000:1. Microscope permits analysis of foreign matter in the micron size range. Foreign matter analysis program as standard Powerful support for foreign matter analysis. Includes more than 300 spectra of recognized foreign matter. fluororesin -0 4000 3000 2000 1500 1000 1/cm Easy maintenance Automatic dryer protects the optical system. Eliminates superfluous maintenance. Infrared spectra of contaminant and fluororesin 12

Food Processing Food processing takes a farmed product and turns it in to an edible, marketable product that may have a longer shelf life. Examples of these processes are baking, canning, fermenting, grilling, heating, hydrolysis, and roasting. During these processes, undesirable and even toxic compounds can form. Or cross-contamination can occur from the big eight allergens of egg, fish, milk, peanuts, shellfish, soy, tree nuts, and wheat to non-allergen foods. Analysis of Acrylamide in Potato Chips (LC-MS/MS) Acrylamide was found to form in fried foods like potato chips via the so-called Maillard reaction of amino acid asparagine and reducing sugar glucose at a higher temperature (120 C). The health risk of acrylamide present in many processing foods became a concern immediately, because it is known that the compound is a neurotoxin and a potential carcinogen to humans. Y= 1.1239X + 0.594168 R2 = 0.9999 Calibration curve (left) and MRM peaks (right) of acrylamide spiked into potato chips matrix, 1-500 ppb with 50 ppb IS (internal standard) added. Analysis of Styrene Leached from Polystyrene Cups (Headspace-GCMS) Worldwide studies have revealed the negative impacts of household disposable polystyrene cups on human health and the environment. Styrene is considered as a possible human carcinogen by the WHO and International Agency for Research on Cancer (IARC). Migration of styrene from polystyrene cups containing beverages has been observed. Styrene enters into our body through the food we eat, mimics estrogens in the body, and can therefore disrupt normal hormonal functions. This could also lead to breast and prostate cancer. 2.00 (x1,000,000) m/z : 104.00 1.75 1.50 1.25 1.00 0.75 0.50 0.25 0.00 50 ppb 20 ppb 10 ppb 5 ppb 2.5 ppb 1 ppb Overlay of SIM chromatograms for styrene m/z 104 at linearity levels 1 ppb to 50 ppb in less than 2.5 minutes 2.2 2.3 2.4 2.5 2.6 (x100,000) m/z : 104.00 min 7.5 5.0 Spiked 2.5 Unspiked 0.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Overlay SIM chromatograms of spiked and unspiked styrene samples min Shimadzu s Total Support for Food Safety Analytical and Testing Instruments for Food Safety 13

Analysis of Flavor & Texture Analysis of Off-Flavor (Headspace GC-MS) 2,4,6-Trichloroanisole (TCA) is a natural compound known as the chief cause of musty smell in wine. Below is an analysis of wine for 1 ng/l of TCA by headspace-trap-gc-ms. The headspace-trap method is 10 times more sensitive then headspace alone when coupled to a GC-MS. Application System of Headspace-Trap GC-MS GCMS-QP2010 Ultra with HS-20 Trap of HS-20 Trap SIM Chromatogram of (TCA) in wine measured with HS-Trap GC/MS at 1 ng/l Multiple modes Loop Mode captures fixed volume for transfer to GC. Multiple Headspace Extraction (MHE) captures the sample from the same vial multiple times, resulting in up to ten GC-MS analyses. Trap Mode increases sensitivity by a factor of 10 by trapping the headspace. Automation 90-position sample tray holds 10 or 20 ml vials. A 12-position equilibration oven prepares upcoming samples. Optional barcode reader available. Flavor Release Evaluation Kit (EZ Test-GC-MS) Evaluation of flavor and texture with each chew is possible. The EZ Test measures the texture with each chew as the flavors are released into the sampling bag. The flavors are extracted from the bag with a SPME sample needle and injected into the GC-MS for flavor analysis. Flavor Release Evaluation Kit EZ Test-GC/MS SPME needle and sampling bag EZ Test GCMS-QP2010 Ultra Evaluation of texture and flavor with each chew Eliminate off-flavor and off-texture of foods. 6.0 5.0 Released by texture Brittle Research & develop new textures and flavors which vary with each bite. 4.0 3.0 2.0 1.0 0.0 8.0 10.0 12.0 14.0 16.0 18.0 GCMS flavor analysis EZ Test texture analysis 14

Prevention of Defects in Packaging Testing the strength and functionality of packaging materials is essential to ensure food products reach consumers fresh and undamaged. To aid the development and quality control testing of packaging materials, Shimadzu analytical and testing technologies allow the analysis of residual solvents in packaging materials, strength testing of adhesive seals, and checks for pin holes in packaged products. Adhesive Strength Testing (Strength Tester) EZ Test Universal Testing Machines A wide range of materials is used for packaging, including aluminum and other metals and non-metallic materials such as paper and plastic. According to their application, these materials must meet certain tensile, compression, and bending strength requirements, and if an adhesive is used, adhesive strength requirements. In the examples below, adhesive strength results for liquid-filled pouch and dry snack package specimens are shown. In both instances, the adhesives meet the requirements of being strong enough to protect the contents, but weak enough so that the contents can easily be accessed by consumers. N40 30 20 1-1 1-2 1-3 10 0 0 5 10 15 20 min Liquid-filled pouch N15 1-1 12 1-2 1-3 9 6 3 Comprehensive range of jigs Diverse strength testing: peeling, tensile, shear, bending, compression, etc. Measures brittle and elastic samples 5 kn max. capacity, 920 mm stroke (long-type). Food texture evaluation Objective testing which analyzes the consistency and mouth feel of foods. 0 0 7 14 21 30 Dry snack packaging min Residual Solvent Analysis (Headspace GC) Gas Chromatograph Headspace Analysis System Quality control of residual organic solvents is required, as the manufacture of food packaging materials and containers involves the use of organic solvents in printing inks and adhesives. These components can be analyzed by headspace GC or by diluting in a solvent and directly injecting this sample solution into a GC instrument. isopropanol methanol MEK ethyl acetate toluene Sample spiked + standard Sample only Headspace GC analysis of residual organic solvent dry snack packaging High sensitivity High-sensitivity analysis of volatile components (low-boiling-point components). Easy maintenance No non-volatile components enter the GC. Reduced analysis time Faster analysis than direct-injection GC for liquid samples containing high-boiling-point components. Shimadzu s Total Support for Food Safety Analytical and Testing Instruments for Food Safety 15

List of Analytical and Testing Instruments for Food Safety Gas Chromatograph (GC) Gas Chromatograph Mass spectrometer (GC-MS) Liquid Chromatograph (LC) Residual Pesticides Mycotoxins Veterinary Pharmaceutical Food Additive Toxic Metals Inspection and Analysis of Foreign Matter Off-Flavor Prevention of Defects in Packaging Region and Identification Type and Observation Identification of Bacteria Shimadzu s Total Support for Food Safety Liquid Chromatograph Mass spectrometer (LC-MS) UV-VIS Spectrophotometer (UV) FTIR Spectrophotometer (FTIR) Atomic Absorption Spectrophotometer (AA) ICP Emission / Spectrometer (ICP, ICP-MS) X-Ray Fluorescence Spectrometer (XRF, EDX) Universal Testing Machine (AG, EZ Test) MALDI-TOF Mass Spectrometer (MALDI-TOF-MS) Shimadzu Balances UniBloc family of balances Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol TM or. Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own. www.shimadzu.com/an/ For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you as is without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. Shimadzu Corporation, 2014 Printed in Japan 3655-10401-50AIT