MiSeq. system applications

Transcription

1 MiSeq system applications

2 Choose your application. Load, and go. Only MiSeq offers a fully integrated sequencing ecosystem. Optimized sample preparation kits, push-button sequencing, and automated data analysis, create the first end-to-end sequencing solution. Delivering the fastest, most accurate results for the widest breadth of applications. And, no need for extra hardware store, analyze, and share data with BaseSpace, Illumina s secure, cloud-based resource. From sample to data in the same day. All at the touch of a button. -Seq 16S Metagenomics

3 mplicon hip-seq g equencing e novo quencing Clone Checking -Seq -Seq -Seq Clone Checking -Seq Metagenomics Library QC sequencing Library QC argeted equencing 16S Metagenomics Regulation 16 Metagen 16S 16S Metagen Metageno Clone Checki Clon Chec Clon Check

4 DNA to data in record time. Nextera DNA Sample Prep Kits Nextera technology combines with the MiSeq system to provide the fastest and easiest solution for amplicon and small genome sequencing. In one quick step, DNA is simultaneously fragmented and tagged with sequencing adapters using standard lab equipment to deliver libraries that can be rapidly sequenced using single 36 bp reads. Fastest time to results Go from DNA to data in less than 8 hours Easiest to use Prepare sequencing-ready samples in 1.5 hours with 15 minutes hands-on time Lowest DNA input Use just 50 ng DNA per sample, enabling use with samples in limited supply Highest sample throughput Index up to 96 samples and use master-mixed reagents to manually process >600 samples per week Prep Sequence Analyze 15 minutes hands-on 20 minutes hands-on fully automated <2 HOURS HOURS HOURS Share secure and store A fully integrated sequencing ecosystem.

5 sequencing transformed. TruSeq Kit Optimized for the MiSeq system, TruSeq offers a highly multiplexed amplicon and sample assay for powerful, fast, and cost-effective variant identification. Samples are processed and ready for sequencing in less than 8 hours with only 2.5 hours of hands-on time. Data analysis is performed directly on the MiSeq system, providing quick, easy-to-interpret results. Simplest assay customization Create and manage projects with the online, user-friendly DesignStudio software Fastest sample processing Prepare and enrich up to 384 amplicons per sample and 96 samples per plate simultaneously using standard lab equipment Shortest time to project completion Go from genomic DNA to fully analyzed data in less than 2 days Easiest analysis Access pre-configured, on-instrument software for automated variant calling and data analysis Using the MiSeq system and the TruSeq assay, an ~10 kb region comprised of 53 overlapping tiled amplicons was sequenced. Three variants were observed in the samples sequenced. Top panel: read depth. Middle panel: quality score, oscillates with single and overlapping reads to provide Q30 and Q40 scores. Bottom panel: variants discovered or validated in the experiment.

6 The widest breadth of sequencing applications. The only personal sequencing system capable of automated paired-end reads. Sample prep kits optimized for a variety of applications. Empower your research with the MiSeq system. Application Recommended Read Length Illumina Solution (tens of targets) 1 36 (hundreds of targets) Hybrid Capture (thousands of targets) S Metagenomics Clone Checking 1 36 De novo 1 36, , s 1 36, Seq (microbial) Quality Control Library QC 2 25 Regulation * 1 36 Use with Ribo-Zero. *Illumina demonstrated protocol coming soon. TruSeq Kit Nextera DNA Sample Prep Kit TruSeq DNA Sample Prep Kit TruSeq Sample Prep Kit TruSeq Sample Prep Kit TruSeq Kit

7 MiSeq system applications More than just a sequencer MiSeq is a complete sequencing ecosystem. With the largest applications portfolio at your fingertips. And, access to an entire community of users and tools with BaseSpace. All designed to move your research forward. The pocket inserts showcase just a few of the applications we ve explored with the MiSeq system. And this is just the beginning. Learn more at

8 FOR RESEARCH USE ONLY Illumina, Inc. All rights reserved. Illumina, illuminadx, BaseSpace, BeadArray, BeadXpress, cbot, CSPro, DASL, DesignStudio, Eco, GAIIx, Genetic Energy, Analyzer, Studio, GoldenGate, HiScan, HiSeq, Infinium, iselect, MiSeq, Nextera, Sentrix, SeqMonitor, Solexa, TruSeq, VeraCode, the pumpkin orange color, and the Genetic Energy streaming bases design are trademarks or registered trademarks of Illumina, Inc. All other brands and names contained herein are the property of their respective owners. Pub. No Current as of 02 February 2012