Agencourt AMPure XP. Xtra Performance Post-PCR clean UP
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1 Agencourt AMPure XP Xtra Performance Post-PCR clean UP
2 Applications o PCR o Genotyping, SNP detection o Fragment analysis o Sequencing (Sanger and Next generation) o Cloning o Primer walking
3 Agencourt AMPure XP: Xtra Performance Chemistry: SPRI based with speed beads Recovery: Double stranded and single stranded DNA with 70 90% of fragment sizes 150bp 10 kb Processing time: Faster than Traditional post PCR clean up methods Purification: Efficient removal of primers, primer dimers, dntps and salts. Stable PCR products post clean up: No PCR degradation at 4 C for 7 days Compatible with down stream applications PCR Genotyping ( SNP detection) Fragment analysis Sequencing (Sanger and Next generation (post shearing clean up) Cloning Fragment analysis Primer walking Automated and scalable: 96, 384 well formats (Biomek 3000, NX P, and FX P ) User friendly automated method o Quick start up menu o Deck set up walk through menu o Method recovery o LIMS compatible Shelf-life: 1 year
4 Agencourt AMPure XP
5 Agencourt AMPure vs. AMPure XP: Beads COOH COOH COOH COOH COOH COOH COOH Magnetite Encapsulation COOH COOH Non-styrene polymer surface
6 Fast and Pure PCR Products Bind Inc Bind settle EtOH settle Air Dry Elute Inc Elute Settle Total Agencourt AMPure 5 min 5 min 30 sec 10 min 2 min 5 min 27.5 min Agencourt AMPure XP 5min 2 min 30 sec 0 min 2 min 1 min 10.5 min
7 Agencourt AMPure XP vs. AMPure: Faster & Higher Throughput Final Transfer Plates Agencourt AMPure XP (min) Agencourt AMPure (min) XP % Faster Yes No % % % % % % % % % %
8 High Recovery Agencourt AMPure XP QIAquick QuickStep Concentration (ng/ul) bp 800bp 10kb Template size (bp) Trade marks are property of their respective owners
9 Purification Versatility : ds & ss DNA Concentration (ng/ul) bp 800 bp 10 kb 0.00 ds DNA ss DNA Agencourt AMPure XP
10 Pure PCR Products: No Dimers --- No Salts M Agencourt AMPure XP QIAquick QuickStep ExoSAP-IT M Agencourt AMPure XP QIAquick QuickStep ExoSAP-IT 2.00 OD260/ Primer Dimers 0.00 No Primer Dimers 200bp 800bp 10kb Template size (bp) No Salts Carry Over Trade marks are property of their respective owners
11 Stable PCR Products M Agencourt AMPure XP QIAquick QuickStep ExoSAP-IT M M Agencourt AMPure XP QIAquick QuickStep ExoSAP-IT M > 50% degradation Day 0 Day 7 at 4 C PCR Products Post PCR Clean up on 1.2% Agarose
12 Faster PCR Clean up Time to Process 96 Samples (min) Agencourt AMPure XP QIAquick QuickStep ExoSAP-IT
13 Agencourt AMPure XP Offering Product Size* Product # Agencourt AMPure XP---5.0mL 139/278 A63880 Agencourt AMPure XP mL 1666/3333 A63881 Agencourt AMPure XP mL 12500/25000 A63882 Method Product # Software - Agencourt AMPure XP 96 MC - v3.x A79794 Software - Agencourt AMPure XP 384 MC - v3.x A79795 * Note: Ampure price per prep dependent on PCR reaction volume. Pricing assumed for 25(96) & and 10(384) ul PCRs.
14 User Friendly Interface
15 Agencourt AMPure XP GUI Quick Start tab Purpose: To provide a simple interface for users to quickly specify common runtime variables and confirm deck setup. Key Features: System Inputs - Number of plates - PCR volume System Outputs - Transfer option - Elution volume - Transfer volume Sample Tracking - User ID - Reagent Lots Display deck layout - Barcode Entry Automatic restoration of previous run values
16 Agencourt AMPure XP GUI Run Setup tab Purpose: To guide novice users through the run setup process. Key Features: Detailed instructions to guide users step by step through the setup process at their own pace. Positions specified in the instructions are highlighted in the deck display. Minimum fill volumes for each reagent reservoir are calculated and displayed (i.e. it takes more reagent to process 4 plates than 1). Completely optional; expert users do not have to go through setup guide to start a run Just Quick Start > Run.
17 Agencourt AMPure XP Method Recovery tab Purpose: To enable users to recover an aborted run. Key Features: The state of each plate (which steps have been performed, which have not) and the location of each plate is maintained throughout the run. If a method recovery file is found when the method starts up, the GUI will present the Method Recovery tab. The list view shows the current state of the highlighted plate. Users can click on any plate in the deck display to highlight it and see its state. Users can manipulate the state of each plate by checking/unchecking the box to the left of each step. Users can rearrange the location of plates in the deck display. Users can reset a plate, all plates, the deck or start over completely with the click of a button. Detailed instructions for this step are provided with the click of a button.
18 Agencourt AMPure XP Customize Procedure tab Purpose To make it easy for users to adjust run settings. It enables users that are not comfortable editing or creating Biomek methods to easily customize an Agencourt method to suit their specific needs. Key Features Every sensible protocol variable is exposed in a descriptive and readable format. Non-validated run settings are highlighted to caution against changes. Validated run settings can be restored with the click of a button. Custom run settings are saved and restored. Allows multiple users to run their own customized version of the method. Settings can be locked. Detailed instructions for this step are provided with the click of a button.
19 Automation Configuration NX P 96 Beckman Part # Qty Decription A Biomek NXp Single Arm Multichannel Laboratory System A Biomek Automation Controller XP and Monitor with Biomek System Software Biomek FX/NX 96-Channel 200 µl disposable tip pipetting head Biomek Disposable Tip Loader ALP The Biomek Disposable Tip Loader ALP for 96/P200 and 384/P30 disposable tips. Requires: 40 PSI clean dry air, 2.5 cfm High-Density Twelve-Position 4x3 ALP For use on a FX/NX Standard Single-Position ALP for positioning of one standard 96 and/or 384 well microtiter plate Biomek Disposable 96 ALP Tip Wash Station. NOTE: Requires Biomek Device Controller (P/N ) Biomek Device Controller Includes Biomek device controller module, CAN communications cable. A Agencourt AMPure 96 Batch method A Installation & validation, North America, 1-day A Agencourt SPRIPlate 96R Super Magnet Plate
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