Syllabus Chemistry 431B Biochemistry Winter Course Prerequisite: Grade of C- or better in Biochemistry I (Chem 431A)

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1 Syllabus Chemistry 431B Biochemistry Winter 2013 Instructor: Jamil Momand, Ph.D. Class location and time: Salazar Hall, C-265 MWF 11:40-12:30 Office Hours: La Kretz Hall, Room 270 M 10-11, T Department phone: (323) Website: Course Prerequisite: Grade of C- or better in Biochemistry I (Chem 431A) Required Texts: Mathews, Van Holde, Appling and Anthony-Cahill, Biochemistry, 4 th edition, Pearson Canada Inc, 2013, (ISBN: ). Library Electronic Res.: Previous exams in Chem 431B. Midterm Exams: Final Exam: 1: Fri, 25 Jan 2: Wed, 20 Feb Mon, 18 Mar, 10:45 am-1:15 pm Grading: Quizzes (2) 40 points Online Homework 60 points Exam points Exam points Final Exam 200 points Total 500 points Make-up Exams: All requests for make-up exams must be accompanied by a signed letter (with appropriate letterhead) from a physician no later than one day after the scheduled exam and must include a phone number for verification. There are no make-up quizzes Dropping the Course: The no record drop period spans from Jan. 7 through Jan. 14. After Jan. 14 you must submit a letter to the instructor from a physician stating that you must drop the course for medical reasons. You will receive a W on your transcript for dropping the course after Jan. 14. Grading Scale: A: 90.0%-100% Page 1 of 6

2 A-: 86.0%-89.9% B+: 82.0%-85.9% B: 78.0%-81.9% B-: 74.0%-77.9% C+: 70.0%-73.9% C: 60.0%-69.9% C-: 54.0%-59.9% D+: 50.0%-53.9% D: 40.0%-49.9% Learning Outcomes Upon completion of this course students will 1) understand some mechanisms of enzyme-mediated reaction catalysis. 2) understand and interpret enzyme kinetic data. 3) understand how inhibitors and activators regulate enzyme catalysis behavior. 4) know how food is converted into energy at the molecular level in most organisms. 5) have an overview of how plants convert light into energy. 6) understand how hormones control storage, synthesis, and breakdown of fat. Book Homework problems. Book Homework problems are optional but are suggested as a way to increase understanding of lecture material. You will not be graded on these problems. Online Homework. Online homework will be assigned through. 1. Go to 2a. If you already have a account, log in, click ""View Available Courses"", then skip to step 3. 2b. If you have Facebook account, you can use it to quickly create a SaplingLearning account. Click "create account" located under the username box, then click "Login with Facebook". The form will auto-fill with information from your Facebook account (you may need to log into Facebook in the popup window first). Choose a password and timezone, accept the site policy agreement, and click "Create my new account". You can then skip to step 3. 2c. Otherwise, click "register here" located under the username box. Supply the requested information and click "Create my new account". Check your (and spam filter) for a message from and click on the link provided in that Find your course in the list (listed by school, course, and instructor) and click the link. 4. Select your payment options and follow the remaining instructions. Once you have registered and enrolled, you can log in at any time to complete or review your homework assignments. During sign up - and throughout the term - if you have any technical problems or grading issues, send an to explaining the issue. The Sapling support team is almost always more able (and faster) to resolve issues than your instructor. A web site with tutorial aids from the textbook publisher is available at Page 2 of 6

3 Academic Dishonesty: The California Code of Regulations, section 41301, states that the following are grounds upon which student discipline can be based: cheating, plagiarism, or other forms of academic dishonesty that are intended to gain unfair academic advantage. If caught cheating, students will get an F grade and will be disciplined by the College of Natural and Social Sciences. Americans with Disabilities Act: Reasonable accommodations are any actions taken to permit a student to: (a) have equally effective communication; or (b) to have equal access to instruction, programs, services and activities sponsored by the University. Providing reasonable accommodation is mandatory when: (a) The student has a protected disability. (Medical documentation may be requested to verify the existence of a protected disability and the need for reasonable accommodation.) (b) The student requests a reasonable accommodation. (c) A reasonable accommodation is required due to a protected disability. (d) It is possible to provide that accommodation without undue hardship. Undue hardship is a fundamental alteration in a program or service or an undue financial or administrative burden. Under the law, in most instances, it will be difficult to justify an exception based on undue hardship. Any situation thought to be an undue hardship must be discussed with the Equity and Diversity Specialist at: (323) (Tel), (323) (TDD), or (323) (FAX). Page 3 of 6

4 Jan. 21, MLK Holiday LECTURE SCHEDULE Biochemistry (Chem 431B) Winter 2013 Lecture Date Topic Reading Homework problems 1 7 Jan Introduction, grading policy, enzymes, rates, first order reactions, second order reactions pp Book , Jan Reaction coordinate diagram, transition state, induced fit model, conformational selection model, Michaelis-Menten kinetics, serine protease 3 11 Jan kcat (turnover number), kcat/k M (enzyme efficiency), Lineweaver-Burk (double reciprocal plot), reversible and irreversible inhibition 4 14 Jan Enzyme classification, cofactors, control of enzyme function, substrate level (product inhibition), feedback control, allostery 5 16 Jan Quiz 1, Covalent modification, metabolism, catabolism, anabolism, biochemical reaction types 6 18 Jan ATP, Energy Charge, energy yield, respiratory quotient, reducing equivalent, coupling reactions Jan MLK Holiday 7 23 Jan Glycolysis, first 5 reactions, the second 5 reactions Jan Midterm 1 Ch s 11, Jan Metabolic fates of pyruvate, lactate metabolism, ethanol metabolism, gluconeogenesis (first step), 9 30 Jan Metabolic fates of pyruvate (continued), oxidation to acetyl CoA pp pp ; pp pp Chapter 12 pp Chapter 12 pp Chapter 13 pp Chapter 13 pp Chapter 14 pp Book-11.8, 11.9, 11.11, Book-11.13a, , Book Book Book , 13.5, 13.7 Book-13.8, 13.12, 13.14, Book-14.8, Page 4 of 6

5 Lecture Date Topic Reading Homework problems 10 1 Feb Gluconeogenesis (cont.), glycogen, regulation of glycolysis and gluconeogenesis, Pasteur effect, Chapter 13 pp Book-13.17, 13.18, 13.20, 13.23, 13.24, 13.26, Feb Citric acid cycle overview, reaction mechanisms 12 6 Feb Citric acid cycle regulation, anapleurotic reactions 13 8 Feb Quiz 2, Mitochondria, oxidative phosphorylation, electron transport chain Feb Standard potentials, P/O ratio, inhibitors of electron transport, electrochemical gradient (proton motive force), Feb Shuttling reducing equivalents, uncouplers, ATP synthase, energy yields, cytochrome c and apoptosis Feb Regulation of oxidative phosphorylation, heat generation, mito. genome Feb Overview of photosynthesis, chloroplast, light reactions, PSI, PSII, cyclic electron flow Chapter 14 pp Chapter 14 pp Chapter 15 pp Chapter 15 pp Chapter 15 pp Chapter 16 pp Book-14.4, 14.6, Book Book-15.1, 15.2, 15.3, 15.4, 15.6, 15.7, 15.13, 15.14, 15.15, Book-16.1, 16.2, Feb Midterm 2-Chapters 13,14, Feb Dark reactions (Calvin cycle), regulation of photosynthesis Chapter 16 pp Feb Photorespiration, C4 cycle Chapter Feb Lipid metabolism overview, the obesity problem, fat transport, bile salts, lipoproteins Chapter 17 pp Mar Cholesterol transport, mobilization of stored fat, fatty acid oxidation overview, fatty acid transport, β-oxidation, compare energy output from glucose and fatty acid Chapter 17 pp Book-16.5, 16.7, 16.9, 16.11, 16.12, Book-17.1, 17.2, 17.3, 17.5, 17.6 Page 5 of 6

6 Lecture Date Topic Reading Homework problems 22 4 Mar Ketogenesis, fatty acid synthesis Chapter 17 Book-17.10, 17.11, pp Mar Control of fatty acid synthesis, biosynthesis of TAGs, hormonal control of obesity 24 8 Mar Role of major organs on control of fuel metabolism overview, brain, muscle, heart, adipose tissue, blood Mar Hormonal regulation of fuel: effects of insulin, glucagon, epinephrine; regulating proteins Mar Metabolic stress, starvation, diabetes v=4qa1m_2hzfm Podcast: Lecture by Ron Evans (HHMI) Mar Review, instructor evaluation Mar Final exam (cumulative) 10:45-1:15 Chapter 17 pp Chapter 18 pp Chapter 18 pp Chapter 18 pp Book-17.14, , 18.2, 18.3, 18.4, , 18.7, 18.8, 18.9 Page 6 of 6

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