ALFREDO DE LA ESCOSURA MUÑIZ Born in Oviedo, Asturias, Spain, 16 th February 1975 Passport number 45434310H Marital status: Single ADDRESS Nanobioelectronics & Biosensors Group. Catalan Institute of Nanotechnology. ETSE - Edifici Q - 2ª planta. Campus UAB. 08193 Bellaterra - Cerdanyola del Vallès. Barcelona, Spain. Phone number: +34935814918. Fax number: +34935813797. E-mail. alfredo.escosura.icn@uab.es. EDUCATION June 2006: PhD. degree entitled Sodium aurothiomalate as electrochemical label in biosensors construction. University of Oviedo (Spain). November 2000: M. Sc. degree entitled Electrocatalytic studies of gold deposited on the surface of carbon electrodes. University of Oviedo (Spain). March 2000: B. Sc. degree in Chemistry, focus in Analytical Chemistry. University of Oviedo (Spain). RESEARCH EXPERIENCE January 2008 Today: Juan de la Cierva post-doctoral researcher in the Nanoscience Institute of Aragon (Zaragoza, Spain) in collaboration with the Nanobioelectronics & Biosensors Group of the Catalan Institute of Nanotechnology (Barcelona, Spain). October 2006 December 2007: Post-doctoral researcher in the Nanobioelectronics & Biosensors Group of the Catalan Institute of Nanotechnology (Barcelona, Spain). October 2002 - June 2006: Research Chemist for the University of Oviedo for the Project BIO2003-06008-C03-01, in order to obtain the Ph. D. degree. October 1999 - November 2000: Research Chemist for the University of Oviedo for the Project FC-02-PC-REC01-05 in order to obtain the M. Sc. degree. - 1 -
PUBLICATIONS - Electrochemical quantification of gold nanoparticles based on their catalytic properties toward hydrogen formation: Application in magnetoimmunoassays. M. Maltez-da Costa, A. de la Escosura-Muñiz, A. Merkoçi, Electrochemistry Communications (2010) in press. - Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms, P. Kara, A. de la Escosura-Muñiz, M. Maltez-da Costa, M. Guix, M. Ozsoz, A. Merkoçi, Biosensors and Bioelectronics (2010) in press. - Gold nanoparticle-based electrochemical magnetoimmunosensor for rapid detection of antihepatitis B virus antibodies in human serum, A. de la Escosura-Muñiz, M. Maltez-da Costa, C. Sánchez Espinel, B. Díaz-Freitas, J. Fernández-Suárez, A. González-Fernández, A. Merkoçi, Biosensors and Bioelectronics (2010) in press. - Immunosensing using nanoparticles, A. de la Escosura-Muñiz, C. Parolo, A. Merkoçi, Materials Today 13 (2010) 24-34. - Label-free voltammetric immunosensor using a nanoporous membrane based platform, A. de la Escosura-Muñiz, A. Merkoçi, Electrochemistry Communications 12 (2010) 859-863. - Electrochemical detection of proteins using nanoparticles: applications to diagnostics, A. de la Escosura-Muñiz, A. Merkoçi, Expert Opinion on Medical Diagnostics 4(1) (2010) 21-37. - Rapid identification and quantification of tumor cells using an electrocatalytic method based on gold nanoparticles, A. de la Escosura-Muñiz, C. Sánchez-Espinel, B. Díaz-Feitas, A. González- Fernández, M. Maltez-da Costa, A. Merkoçi, Analytical Chemistry 81 (2009) 10268-10274. - Controlling the electrochemical deposition of silver onto gold nanoparticles: Reducing interferences and increasing the sensitivity of magnetoimmuno assays, A. de la Escosura-Muñiz, M. Maltez-da Costa, A. Merkoçi, Biosensors and Bioelectronics 24 (2009) 2475-2482. - ICP-MS: a powerful technique for quantitative determination of gold nanoparticles without previous dissolving, R. Allabashi, W. Stach, A. de la Escosura-Muñiz, L. Liste-Calleja, A. Merkoçi, Journal of Nanoparticle Research 11 (2009) 2003 2011. - Electrochemical analysis with nanoparticle-based biosystems, A. de la Escosura-Muñiz, A. Ambrosi, A. Merkoçi, Trends in Analytical Chemistry 27 (2008) 568-584. - Silver, gold and the corresponding core shell nanoparticles for sensing applications, F. Douglas, R. Yañez, J. Ros, S. Marín, A. de la Escosura-Muñiz, A. Merkoçi, Journal of Nanoparticle Research 10 (2008) 97 106. - 2 -
- DNA hybridization biosensors using polylysine modified SPCEs, M. Díaz González, A. de la Escosura-Muñiz, M.B. González-García, A. Costa-García, Biosensors and Bioelectronics 23 (2008) 1340-1346. - DNA hybridization biosensor based on aurothiomalate electroactive label on glassy carbon electrodes, A. De la Escosura-Muñiz, M.B. González-García, A. Costa-García, Biosensors and Bioelectronics 22 (2007) 1048-1054. - Aurothiomalate as an electroactive label for the determination of Immunoglobulin M, using glassy carbon electrodes as immunoassay transductors, A. De la Escosura-Muñiz, M.B. González-García, A. Costa-García, Sensors and Actuators B 114 (2006) 473-481. - Determination of human serum albumin using aurothiomalate as electroactive label, A. De la Escosura-Muñiz, M.B. González-García, A. Costa-García, Analytical and Bioanalytical Chemistry 384 (2006) 742-750. - Electrocatalytic detection of aurothiomalate on carbon electrodes. Application as a nonenzymatic label to the quantification of proteins, A. De la Escosura-Muñiz, M.B. González- García, A. Costa-García, Analytica Chimica Acta 524 (2004) 355-363. - Catalytic effect on silver deposition of gold deposited on carbon electrodes, A. De la Escosura-Muñiz, M.B. González-García, A. Costa-García, Electroanalysis 16 (2004) 1561-1568. BOOK CHAPTERS - Nanomaterials for Electroanalysis, A.Merkoçi, A. Ambrosi, A. de la Escosura-Muñiz, B. Pérez, M. Guix, M. Maltez, S. Marin. Encyclopedia of Analytical Chemistry. Editorial: John Wiley & Sons Ltd (R.A. Meyer and J. Wiley eds), Chichester, UK. DOI: 10.1002/9780470027318.a9077. Published 15 June 2010. - Nanoparticles and inductively coupled plasma mass spectroscopy based biosensing, A. Merkoçi, R. Allabashi, A. de la Escosura Muñiz, Biosensing using nanomaterials. Editorial: John Wiley & Sons Ltd (Arben Merkoçi ed), Hoboken, New Jersey, USA. Chapter 12 (2009) 355-376. - Gold nanoparticles: A versatile label for affinity electrochemical biosensors, A. Ambrosi, A. Merkoçi, A. de la Escosura Muñiz, M.T. Castañeda, Biosensing using Nanomaterials. Editorial: John Wiley & Sons Ltd (Arben Merkoçi ed.), Hoboken, New Jersey, USA Chapter 6 (2009) 177-197. - 3 -
- Electrochemical immunosensing using micro and nanoparticles, A. de la Escosura-Muñiz, A. Ambrosi, S. Alegret, A. Merkoçi, Methods in Molecular Biology: Biosensors and Biodetection. Editorial: Humana Press (Avraham Rasooly and Keith E. Herold eds). Chapter 10 Vol. 504 (2009) 145-155. PATENTS - Method for cell identification and quantification with gold nanoparticles through hydrogen ion reduction, A.Merkoçi, A. de la Escosura-Muñiz, A. González-Fernández, B. Díaz-Freitas, C. Sánchez-Espinel. Spanish Application P200803275 (12/11/08). International Application PCT/ES2009/070489 (10/11/09). International Publication WO 2010/055183 A1 (20/05/10). - Uso de complejos de oro como marcas de moléculas, A. De la Escosura Muñiz, M.B. González García, A. Costa García. Concession number ES2303405 (19/05/09). INVITED LECTURES - Nano-sensing: Biosensors and Nanodiagnosis, Exploratory Workshop: BioNanotechnology: development and application of principles of nano- and bio-sciences to sensing, diagnostics and therapy (European Science Foundation), Sintra (Portugal) 31 August 3 September 2009. - Biosensores electroquímicos nanoestructurados, Summer School Nanociencia y Nanotecnología Analíticas (Universidad de Castilla-La Mancha), Ciudad Real (Spain), 7-9 July 2009. Directors: Prof Ángel Ríos (Universidad de Castilla-La Mancha) and Prof. Miguel Valcárcel (Universidad de Córdoba). - Biosensores electroquímicos basados en nanopartículas y nanomateriales: aplicaciones en biomedicina, Summer School Nanotecnologías en Biomedicina (Universidad Internacional Menéndez-Pelayo), A Coruña (Spain), 20-24 July 2009. Director: José Rivas Rey (Universidad de Santiago de Compostela). - 4 -
CONTRIBUTION TO CONGRESSES - Poster presentation: Protein detection using gold nanoparticles as labels and electrochemical sensing routes 13 th International Conference on Electroanalysis (ESEAC 2010), Gijón (Spain), 20-24 June 2010. - Poster presentation: Scanning electrochemical microscopy studies of nano and microstructurated platforms with interest for biosensing applications 13 th International Conference on Electroanalysis (ESEAC 2010), Gijón (Spain), 20-24 June 2010. - Oral presentation: Nanoparticle based enhancement of electrochemical DNA hybridization signal using nanoporous electrodes 20 th Biosensors World Congress, Glasgow (United Kingdom), 26-28 May 2010. - Poster presentation: Gold nanoparticle-based electrochemical magnetoimmunosensors for rapid detection of clinical biomarkers 20 th Biosensors World Congress, Glasgow (United Kingdom), 26-28 May 2010. - Poster presentation: Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms 20 th Biosensors World Congress, Glasgow (United Kingdom), 26-28 May 2010. - Poster presentation: Nanomaterials based biosensors for cost effective cancer diagnostics Towards Personalized Cancer Medicine (The New York Academy of Sciences), Barcelona (Spain), 19-21 May 2010. - Oral presentation: Investigation of electrochemical responses of different sized gold nanoparticles using screen-printed carbon electrodes XIV Trobada Transfronterera sobre Sensos i Biosensors, Banyuls-Sur-Mer (France), 24-25 September 2009. - Oral presentation: An electrochemical biosensor design for protein detection based on aptamers XIV Trobada Transfronterera sobre Sensos i Biosensors, Banyuls-Sur-Mer (France), 24-25 September 2009. - Poster presentation: Direct electrical detection of point mutations at multi walled carbon nanotube modified electrodes XIV Trobada Transfronterera sobre Sensos i Biosensors, Banyuls-Sur-Mer (France), 24-25 September 2009. - Poster presentation: Immunosensor to identify tumor cells: a novel low cost and efficient device based on electrocatalytic effect induced by gold nanoparticles conjugated to antibodies II European Congress of Immunology, Berlin (Germany), 13-16 September 2009. - 5 -
- Oral presentation: Nanomaterial based electrochemical transducing platforms for biomedical applications Medical Physics and Biomedical Engineering World Congress 2009, Munich (Germany) 7-12 September 2009. - Oral presentation: Clinical applications of gold nanoparticle based magnetobiosensors XXIV Trobades Cientifiques de la Mediterània, Mahón (Spain), 6-8 October 2008. - Oral presentation: Electrochemical bioassays based on gold nanoparticle tags XIII Trobada Transfronterera sobre Sensos i Biosensors, Andorra la Vella (Andorra), 18-19 September 2008. - Poster presentation: Electrochemical routes for fast and efficient detection of biomolecules based on gold nanoparticles II Workshop sobre Nanociencia y Nanotecnología Analíticas, Tarragona (Spain), 25-26 September 2008. - Poster presentation: Direct electrochemical detection of gold nanoparticles: application in magnetobiosensors Trends in Nanotechnology 2008, Oviedo (Spain), 1-5 September 2008. - Poster presentation: Inmunodetección electroquímica de interferon gamma humano mediante el uso de nanopartículas de oro y micropartículas magnéticas, XXXIV Congreso Nacional de la Sociedad Española de Inmunología, Palma de Mallorca (Spain), 21-24 May 2008. - Poster presentation: Biosensor electroquímico para la inmunodetección de anticuerpos específicos frente al antígeno de superficie del virus de hepatitis B (HBsAg), XXXIV Congreso Nacional de la Sociedad Española de Inmunología, Palma de Mallorca (Spain), 21-24 May 2008. - Oral presentation: Immunosensing based on gold nanoparticles Workshop Nanobiosensors for Biomedical applications, Barcelona (Spain), 19-20 December 2007. - Oral presentation: Electrochemical magnnetobiosensing based on gold nanoparticles European Nanosytems 07, Paris (France), 3-4 December 2007. - Oral presentation: Electrocatalytical biosensing based on gold nanoparticles XII Trobada Transfronterera sobre Sensors i Biosensors, Céret (France), 27-28 September 2007. - Poster presentation: Gold nanoparticles for immunosensing, I Workshop sobre nanotecnología Analítica, Córdoba (Spain), 16-17 July 2007. - Poster and oral presentation: Electrocatalytical immunosensing methods based on gold nanoparticles Asiasense 2007, Manila (Filipinas), 5-7 June 2007. - Poster presentation: Electrocatalytical immunosensing methods based on gold nanoparticles, 4º Workshop Nanospain, Sevilla (Spain), 12-15 March 2007. - 6 -
- Oral presentation: Genosensor based on aurothiomalate electroactive label on carbon electrodes, XI Jornadas de Análisis Instrumental, Barcelona (Spain), 15-17 November 2005. - Poster presentation: Aurothiomalate as an electroactive label for monitoring immunological reactions on glassy carbon electrodes, Euroanalysis XIII, Salamanca (Spain), 5-10 September 2004. - Poster presentation: Detección electrocatalítica de aurotiomalato sobre electrodos de carbono. Aplicación como marca no enzimática para la cuantificación de proteínas, XIII Reunión de la Sociedad Española de Química Analítica, A Coruña (Spain), 21-24 October 2003. COURSES - Assistance to the 2nd Spanish Workshop on Nanolithography (Universidad Autónoma de Barcelona), Barcelona (Spain), 10-12 November 2008. - Assistance to the Seminar: La Governança del Risc en les Nanotecnologies (Institut Català de Nanotecnologia), Barcelona (Spain), 5 November 2008. - Assistance to the Nano2Life Industry Workshop (Nano2 life), Barcelona (Spain), 6 May 2008. - Assistance to the Summer School Nanotecnologías en Biomedicina (Universidad de Barcelona), Barcelona (Spain), 14-18 July 2008. - Assistance to the International Symposium: Nanotechnology in medicine: diagnosis and therapy (Fundación Ramón Areces), Vigo (Spain), 12-13 November 2007. - Assistance to the International Symposium: Nanotechnology implications on health and environment (Fundación Ramón Areces), Barcelona (Spain), 29 October 2007. - Assistance to Jornadas de Nanotecnologías en Biomedicina (Nanoaracat), Jaca (Spain), 9-13 July 2007. - Assistance to Jornadas de Aplicaciones Industriales de la Nanotecnología (Nanoaracat), Barcelona (Spain), 14 May 2007. -Assistance to Jornada de Microscòpies de proximitat (ICMAB-CSIC), Barcelona (Spain), 20 April 2007. - Assistance to Jornadas Científicas Nanoaracat, (Nanoaracat), Barcelona (Spain), 28-29 March 2007. - 7 -
- Assistance to Jornada de Microscopías de Sonda Local (Nanoaracat), Zaragoza (Spain), 12 December 2006. - Post-graduate courses. University of Oviedo. Period 2002-2003: - Environmental and sanitary microbiology. - Electrochemical sensors. - Electroanalytical techniques in clinical and environmental analysis. - Trends in the development of materials for optical recognition of chemical species. - Analysis and profiles of surfaces by spectroscopic techniques. - HPLC detectors. Trends and applications. - Separation and determination of enantiomers by HPLC and gas chromatography. Other courses: November 2004 - July 2005: Technical Master in Milky Industries, 659 hours. University of Oviedo. June 2003: University teaching formation: programming, methodology and evaluation. University of Oviedo. March 2003: Certificate of Pedagogic Qualification. University of Oviedo. April 2000: Norma ISO 9000. Economy of quality and statistic control of processes. University of Oviedo. WORKING EXPERIENCE January 2008 Today: Post-doctoral researcher in the Aragon Institute of Nanoscience. Zaragoza (Spain). October 2006 December 2007: Post-doctoral researcher in the Nanobioelectronics & Biosensors Group of the Catalan Institute of Nanotechnology. Barcelona (Spain). - 8 -
February 2005-September 2005: Work in practices for the Environmental, Quality Control and I+D+i departments of the industry Central Lechera Asturiana S.A.. Siero and Villaviciosa, Asturias (Spain). March 2001- February 2002: Environmental Consultant in Servicios Medioambientales Asturiano, S.L (EcoIndustria Group). Oviedo (Spain). July 1999- October 1999: Work in practices for the Quality Control Laboratory of the industry Chocolates LACASA S.A.. Siero, Asturias (Spain). LANGUAGE SKILLS Spanish: mother tongue. Good level of both written and spoken english. Good level of both written and spoken catalan. COMPUTER SKILLS MS DOS, Windows (Office, ChemDraw, Harvard Graphics, Corel, Lotus 123), Internet (Netscape, Internet explorer), electronic mail (QVT-Term, Lotus Notes, Microsoft Outlook express, Netscape communicator). September 2010-9 -