Thomas Liehr Editor Fluorescence In Situ Hybridization (FISH) - Application Guide & Springer
Contents Part I Molecular Cytogenetics, Probes and Equipment 1 Molecular Cytogenetic Applications in Diagnostics and Research: An Overview 3 Nedime Serakinci and Steen K0lvraa 2 Molecular Cytogenetics: The Standard FISH and PRINS Procedure 23 Thomas Liehr and Franck Pellestor 3 Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes 35 Fengtang Yang, Vladimir Trifonov, Bee Ling Ng, Nadezda Kosyakova, and Nigel P. Carter 4 1SH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results 53 Anja Weise, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Madeleine Gross, Vivien Klaschka, and Thomas Liehr 5 PNA-FISH Technique for In Situ Assessment of Aneuploidy 61 Franck Pellestor, Petra Paulasova, and Samir Hamamah 6 Oligonucleotide FISH Probes 67 Sean Bradley, Leah Zamechek, and Joan Aurich-Costa 7 Microscopy and Imaging Systems 75 Ivan lourov
8 Optical Filters for FISH 85 Michael Sommerauer and Ingrid Feuerbacher 9 FISH With and Without COT1 DNA 99 Vladimir A. Trifonov, Nadezhda N. Vorobieva, and Willem Rens Part II FISH Applications in Different Tissue Preparations 10 Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblasts 113 Anja Weise and Thomas Liehr 11 Prenatal Diagnostics on Uncultured Amniocytes 123 Bernd Eiben, Ralf Glaubitz, and Hans-Jiirgen Arens 12 Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears 131 Anita Heller, Madeleine Gross, Vera Witthuhn, Katja Behr, Sophie Hinreiner, Hasmik Mkrtchyan, and Thomas Liehr 13 Application of FISH to Archival Cytogenetic Slides 137 Thomas Liehr and Erich Gebhart 14 Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Root Cells or Urine 141 Kristin Mrasek, Anja Weise, Madeleine Gross, Hasmik Mkrtchyan, and Thomas Liehr 15 Characterization of Archived Formalin-Fixed/ Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction 147 Thomas Liehr and Marina Manvelyan 16 FISH on Sperms, Spermatocytes and Oocytes 157 Maria Oliver-Bonet Part III Characterization of Marker Chromosomes 17 Multiplex FISH and Spectral Karyotyping 175 Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Joana Melo, Anna Polityko, and Lukrecija Brecevic
18 Tips and Tricks for mfish 183 Karin M. Greulich-Bode, Maren Kaufmann, Klaus Gilbertz, and Roderick A.F. MacLeod 19 Simultaneous Fluorescence Immunostaining and FISH 193 Christine J. Ye, Lesley Lawrenson, Guo Liu, Joshua Stevens, Steve Bremer, Karen J. Ye, and Henry H.Q. Heng 20 Color-Changing Karyotyping (CCK), an M-FISH/SKY Alternative 217 Octavian Henegariu 21 Three-Color FISH for the Detection of Individual Radiosensitivity 231 Luitpold Disiel, Ulrike Keller, and Susann Neubauer 22 FISH Banding Techniques 243 Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Heike Nelle, Vladimir Trifonov, Marina Manvelyan, and Anja Weise 23 cenm-fish Approaches 251 Nadezda Kosyakova, Anja Weise, Kristin Mrasek, Hasmik Mkrtchyan, Vivien Klaschka, Elisabeth Ewers, Daniela Reich, Nadine Riidiger, and Thomas Liehr Part IV Characterization of Cryptic and Epigenetic Aberrations 24 Subtelomeric and/or Subcentromeric Probe Sets 261 Anja Weise, Kristin Mrasek, Madeleine Gross, Vivien Klaschka, and Thomas Liehr 25 Quantitative DNA Fiber Mapping 269 Chun-Mei Lu, Mei Wang, Karin M. Greulich-Bode, Jingly F. Weier, and Heinz-Ulli G. Weier 26 Parental Origin Determination FISH: Pod-FISH 293 Anja Weise, Kristin Mrasek, Hasmik Mkrtchyan, Madeleine Gross, Sophie Hinreiner, and Thomas Liehr
Part V Interphase FISH and Characterization of the Nuclear Architecture 27 Interphase FISH: Detection of Intercellular Genomic Variations and Somatic Chromosomal Mosaicism 301 Ivan Y. Iourov, Svetlana G. Vorsanova, Ilia V. Soloviev, and Yuri B. Yurov 28 Three-Dimensional Interphase Analysis Enabled by Suspension FISH.. 313 Friederike Hunslig, Marina Manvelyan, Samarth Bhatt, Ulf Steinhaeuser, and Thomas Liehr Part VI Applications of FISH in Zoology, Botany and Microbiology 29 Animal Probes and ZOO-FISH 323 Fengtang Yang and Alexander S. Graphodalsky 30 FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast 347 Harry Scherthan 31 FISH on Insect Cells Transfected with Heterologous DNA: A Single-Cell-Directed Approach 361 Thomas Liehr 32 FISH on Plant Chromosomes 365 Peng Zhang and Bernd Friebe 33 FISH in Food Microbiology 395 Benedetta Bottari, Danilo Ercolini, Monica Gatti, and Erasmo Neviani Part VII Comparative Genomic Hybridization (CGH) and Array CGH 34 Micro-CGH: Microdissection-Based Comparative Genomic Hybridization 411 Anita Heller and Thomas Liehr 35 Array CGH: Opening New Horizons 421 Joris R. Vermeesch Part VIII Internet Links for FISH 36 FISH and Chips on the Internet 441 Thomas Liehr and Anja Weise Index 447