Touch DNA and DNA Recovery. H. Miller Coyle

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Touch DNA and DNA Recovery. H. Miller Coyle"

Transcription

1 Touch DNA and DNA Recovery 1

2 2 What is the link between cell biology & forensic science? Cells are the trace substances left behind that can identify an individual. Cells contain DNA. There are two forms of DNA nuclear and mitochondrial. Most forensic science laboratories are accredited or certified to perform nuclear DNA tests fragment length analysis. A fewer number of forensic laboratories are accredited for the more specialized and time consuming mitochondrial DNA tests sequencing.

3 3 Question #1: How many cells are there in a crime scene sample? This is a comparative exercise that can be performed with basic microscopy skills and just a few chemicals to visualize what is being collected at a crime scene or from reference samples for DNA.

4 4 A basic human cell A membrane-bound compartment of proteins, lipids, carbohydrates, DNA, RNA, and water. Carefully balanced to create an individually functioning unit that can also integrate and function as tissues and organs. Tissues and organs make up the organism.

5 5 Forensic sampling DNA is the same in most all cells and tissues collected from the same individual. Rare tissue specific mutations can occur but these are rare events. Trace biological evidence can be cells from touching objects, shed or forcibly removed hairs, body fluids and all will have the same DNA profile. The difficulty with conclusively identifying an individual by DNA occurs when partial results or complex DNA mixtures are obtained.

6 6 What factors affect DNA results? At a crime scene, there are environmental factors beyond the control of the laboratory. These factors include: bacteria, sunlight, humidity, temperature, drying time, pressure applied during deposit, DNase activity etc. all of which will ultimately determine the quantity and quality of DNA recovered. These factors result in either no profiles, full DNA profiles or partial DNA profiles being obtained.

7 7 Example buccal reference samples (>50 cells); these are the types of samples collected for crime scene comparison and DNA databases. Human Cell Cells stained on a glass slide Membrane Cytoplasm Nucleus Mitochondria (not visible)

8 8 Karyotype digitized image of the chromosomes from a single cell; each fragment of DNA is on a separate chromosome. Human DNA Packaged into 23 pairs of chromosomes inside the nucleus. In DNA extraction methods, both the cell and nuclear membrane need to be ruptured to release the DNA for purification.

9 9 Chromosomes are not visible in the cell s nucleus not even under a microscope when the cell is not dividing. However, the DNA that makes up chromosomes becomes more tightly packed during cell division and is then visible under a microscope. Human DNA Wrapped around histone proteins and folded into these structures.

10 10 Nuclear DNA Targeting different points of DNA (deoxyribonucleic acid)on those 23 pairs of chromosomes. Sugar-phosphate backbone (blue ribbons) linked by nucleotide bases (A-T; G-C)

11 Overview of Steps Involved in DNA Typing 11 AME L D3 TH01 D5 VWA D1 3 D2 1 D7 D8 TPOX D1 6 D1 8 CSF FGA Penta Penta ED Blood Stain PCR Amplification with Fluorescent STR Kits and Separation with Capillary Electrophoresis DNA Quantitation using Real-Time PCR Genotyping

12 12 Sampling has changed in the past 10 years for DNA A bloodstain the size of a quarter used to be required for older methods such as RFLP (restriction fragment length polymorphisms). Current methods use fluorescence, a more sensitive detection method, and can process DNA from 1-20 cells. This process is STR (short tandem repeat analysis).

13 13 Sufficient quantity & quality DNA example: 15 locus profile suitable for CODIS database entry which only requires 13 core loci Figure 1: GeneScan software electropherogram showing AmpFLSTR Identifiler PCR Amplification Kit results for 15 STR loci and the Amelogenin locus analyzed on the ABI PRISM 310 Genetic Analyzer. DNA fragments are labeled in 6-FAM dye (blue), VIC dye (green), NED dye (yellow, depicted in black), and PET dye (red). The GeneScan -500 size standard is labeled with LIZ dye (orange). Partial profiles are similar but missing information at some of these sites.

14 14 Standard cotton swab Sterile. Cost-effective. Available at any pharmacy. Insert in mouth and rub inside of cheek area times. Air dry 5-10 minutes Ḥ. Miller Coyle

15 15 Create a slide smear Rehydrate the swab with 2-5 drops of sterile water Place swab in a clean plastic tube Add more water to cover the tip of the swab (cotton area) Gently agitate the swab so cells are released into the water solution An optional centrifugation step can concentrate the cells into a pellet at bottom of tube Plastic pipette can be used to transfer sample to slide Air dry and view under microscopy

16 Epithelial cells are avascular therefore they rely on cells underneath to nourish them; they are shed from skin surfaces on a continuous basis while the nucleus is also breaking down. Number of nucleated cells recovered will depend on where you sample from (cheek vs. fingerprint). 16

17 17 Cheek cells (non-keratinized stratified squamous epithelium) at 500x magnification; nuclear fast red stain.

18 18 Epithelial cells or epithelium Epithelial tissues line the surfaces of structures throughout the body, and also form many glands. Functions of epithelial cells include secretion, selective absorption, protection, transcellular transport and detection of sensation.

19 19 Traditional cell biology Purpose: To allow the student to consider the biology behind what we do in evidence collection. Swabs are used for: Blood Urine Semen Saliva Touch DNA (cells in fingerprints or from handled items)

20 20 Factors affecting cell recovery Before collection Total number of cells available initially. Environment can affect preservation. Genetic differences. After collection Drying time can affect quality of cell recovery. Rehydration step can affect cell recovery.

21 21 Buccal epithelial cell Nucleus is present Outer membrane is clear, slightly folded on one side Granular cytoplasm is clear C. albicans (yeast) is also present (thats not typical) as thin tube-like structures Bacteria are visible

22 22 This is typically straight forward for cell recovery from buccal samples Sometimes beautiful cell slides are the outcome; other times, cells are present but crumpled due to dehydration, mechanical pressure during slide preparation or over-rinsing.

23 23 Cells from fingerprints Touch DNA Cells or free DNA Perspiration Trace amount of biological material You can place a print on a slide and survey for oils and transferred cells

24 24 Example of fingerprint on glass slide Cells will be visible after staining but may not contain a nucleus. Cell counts may vary from 1-2 cells - >100 cells (non shedder vs. shedder status). Factors affecting shedding: lotion, genetics, exercise, time of day, etc. (varied).

25 25 Single thumbprint on a slide

26 26 Some Applications to Forensic Science Homicides clothing, bodies Property Crimes Stolen items (e.g. wallets; jewelry) Disposed of items (e.g. frisking policies; pawned items) Weapons (knives, ammunition, firearms) Swabs of handles, blades Swabs of cartridges Swabs of trigger, grip, end of barrel, backstrap

27 27 Firearms Who touched or handled? Who loaded weapon? Who fired weapon? Is victim detected on weapon?

28 28 Knives Who handled knife? (touch DNA) Any sign of victim on knife? (handle, hilt, blade)

29 29 Some questions that need to be addressed for the courts: Whose DNA is detected? At what locations on item? Are multiple individuals (DNA mixtures) detected? How long would one have to handle the object for transfer? Is DNA likely from direct or indirect transfer? What can be determined about circumstances based on DNA?

Forensic DNA Testing Terminology

Forensic DNA Testing Terminology Forensic DNA Testing Terminology ABI 310 Genetic Analyzer a capillary electrophoresis instrument used by forensic DNA laboratories to separate short tandem repeat (STR) loci on the basis of their size.

More information

6 Body Fluid Stains and Standards

6 Body Fluid Stains and Standards 6 Body Fluid Stains and Standards Laboratory examination of body fluids (i.e., blood, semen, saliva, etc.) may produce significant information in certain investigations. This chapter considers the recognition,

More information

One of the most important developments in the field of forensic

One of the most important developments in the field of forensic Chapter 5 DNA Evidence and Standardsrds One of the most important developments in the field of forensic science in the past few decades has been the implementation of DNA testing. With this testing, crime

More information

Evidence Preservation in Sexual Assault: Between the Crime Scene and the Medical Examination

Evidence Preservation in Sexual Assault: Between the Crime Scene and the Medical Examination Evidence Preservation in Sexual Assault: Between the Crime Scene and the Medical Examination Pacific Police Development Program Global Justice Solutions LOCARD S PRINCIPLE VICTIM CRIME SCENE OFFENDER Evidence

More information

Mitochondrial DNA Analysis

Mitochondrial DNA Analysis Mitochondrial DNA Analysis Lineage Markers Lineage markers are passed down from generation to generation without changing Except for rare mutation events They can help determine the lineage (family tree)

More information

CRIME SCENE REMINDER CARD. www.icsia.org. "Interview, Examine, Photograph, Sketch, Process"

CRIME SCENE REMINDER CARD. www.icsia.org. Interview, Examine, Photograph, Sketch, Process CRIME SCENE REMINDER CARD "Interview, Examine, Photograph, Sketch, Process" PRIMARY SCENE SECONDARY SCENE BURGLARY CHECK LIST 1. Photograph 7. Fingerprints (Latents) 2. Tool Marks 8. Glass Standards 3.

More information

Willmar Public Schools Curriculum Map

Willmar Public Schools Curriculum Map Subject Area Science Senior High Course Name Forensics Date June 2010 Timeline Content Standards Addressed Skills/Benchmarks Essential Questions Assessments 1-2 Introduction History and Development of

More information

DNA as a Biometric. Biometric Consortium Conference 2011 Tampa, FL

DNA as a Biometric. Biometric Consortium Conference 2011 Tampa, FL DNA as a Biometric Biometric Consortium Conference 2011 Tampa, FL September 27, 2011 Dr. Peter M. Vallone Biochemical Science Division National Institute of Standards and Technology Gaithersburg, MD 20899

More information

Crime Scene and Case Processing. Biological Screening

Crime Scene and Case Processing. Biological Screening Crime Scene and Case Processing Crime Scene and Case Processing Includes: Types of evidence Crime scenes Location and collection of evidence Collection techniques Preservation of evidence Packaging and

More information

The Chinese University of Hong Kong School of Life Sciences Biochemistry Program CUGEN Ltd.

The Chinese University of Hong Kong School of Life Sciences Biochemistry Program CUGEN Ltd. The Chinese University of Hong Kong School of Life Sciences Biochemistry Program CUGEN Ltd. DNA Forensic and Agarose Gel Electrophoresis 1 OBJECTIVES Prof. Stephen K.W. Tsui, Dr. Patrick Law and Miss Fion

More information

Annex to the Accreditation Certificate D-PL-13372-01-00 according to DIN EN ISO/IEC 17025:2005

Annex to the Accreditation Certificate D-PL-13372-01-00 according to DIN EN ISO/IEC 17025:2005 Deutsche Akkreditierungsstelle GmbH German Accreditation Body Annex to the Accreditation Certificate D-PL-13372-01-00 according to DIN EN ISO/IEC 17025:2005 Period of validity: 26.03.2012 to 25.03.2017

More information

Forensic. Sciences. Forensic Sciences. Specialties. Programs. Career Pathways

Forensic. Sciences. Forensic Sciences. Specialties. Programs. Career Pathways Forensic Sciences Specialties Programs Prof. R. E. Gaensslen Director of Graduate Studies Forensic Science University of Illinois - Chicago Career Pathways Forensic Sciences 1 The Hype... the TV version

More information

Crime Scenes and Genes

Crime Scenes and Genes Glossary Agarose Biotechnology Cell Chromosome DNA (deoxyribonucleic acid) Electrophoresis Gene Micro-pipette Mutation Nucleotide Nucleus PCR (Polymerase chain reaction) Primer STR (short tandem repeats)

More information

The Techniques of Molecular Biology: Forensic DNA Fingerprinting

The Techniques of Molecular Biology: Forensic DNA Fingerprinting Revised Fall 2011 The Techniques of Molecular Biology: Forensic DNA Fingerprinting The techniques of molecular biology are used to manipulate the structure and function of molecules such as DNA and proteins

More information

DNA and Forensic Science

DNA and Forensic Science DNA and Forensic Science Micah A. Luftig * Stephen Richey ** I. INTRODUCTION This paper represents a discussion of the fundamental principles of DNA technology as it applies to forensic testing. A brief

More information

Centre of Forensic Sciences Investigators & Submitters Technical Information Sheets

Centre of Forensic Sciences Investigators & Submitters Technical Information Sheets Centre of Forensic Sciences Investigators & Submitters Technical Information Sheets Body Fluid Information Introduction Examinations are conducted based on their scientific merit in the context of the

More information

Collecting a Buccal Swab An Art or a Cinch? By Chantel Marie Giamanco, Forensic Scientist Human Identification Technologies, Inc.

Collecting a Buccal Swab An Art or a Cinch? By Chantel Marie Giamanco, Forensic Scientist Human Identification Technologies, Inc. Collecting a Buccal Swab An Art or a Cinch? By Chantel Marie Giamanco, Forensic Scientist Human Identification Technologies, Inc. An increasing number of cases tried in the courtroom involve DNA evidence.

More information

Lab 5: DNA Fingerprinting

Lab 5: DNA Fingerprinting Lab 5: DNA Fingerprinting You are about to perform a procedure known as DNA fingerprinting. The data obtained may allow you to determine if the samples of DNA that you will be provided with are from the

More information

DNA PROFILING IN FORENSIC SCIENCE

DNA PROFILING IN FORENSIC SCIENCE DA PROFILIG I FORESIC SCIECE DA is the chemical code that is found in every cell of an individual's body, and is unique to each individual. Because it is unique, the ability to examine DA found at a crime

More information

CITY OF CHESTERFIELD POLICE DEPARTMENT GENERAL ORDER 122-11 EFFECTIVE: MAY 5, 2011 CANCELS: GENERAL ORDER 122-07

CITY OF CHESTERFIELD POLICE DEPARTMENT GENERAL ORDER 122-11 EFFECTIVE: MAY 5, 2011 CANCELS: GENERAL ORDER 122-07 CITY OF CHESTERFIELD POLICE DEPARTMENT GENERAL ORDER 122-11 EFFECTIVE: MAY 5, 2011 CANCELS: GENERAL ORDER 122-07 TO: ALL PERSONNEL INDEX AS: DNA EVIDENCE COLLECTION OF EVIDENCE EVIDENCE - DNA CRIME SCENE

More information

A Simplified Guide To DNA Evidence

A Simplified Guide To DNA Evidence A Simplified Guide To DNA Evidence Introduction The establishment of DNA analysis within the criminal justice system in the mid- 1980s revolutionized the field of forensic science. With subsequent refinement

More information

Biology Behind the Crime Scene Week 4: Lab #4 Genetics Exercise (Meiosis) and RFLP Analysis of DNA

Biology Behind the Crime Scene Week 4: Lab #4 Genetics Exercise (Meiosis) and RFLP Analysis of DNA Page 1 of 5 Biology Behind the Crime Scene Week 4: Lab #4 Genetics Exercise (Meiosis) and RFLP Analysis of DNA Genetics Exercise: Understanding how meiosis affects genetic inheritance and DNA patterns

More information

Cell Biology Prokaryotic and eukaryotic cells

Cell Biology Prokaryotic and eukaryotic cells Cell Biology Prokaryotic and eukaryotic cells Observation of cells and organelles In this lab you will be looking at an example of a Prokaryotic cell (Bacillus cereus) and a some examples of Eukaryotic

More information

Forensic Science. The student will demonstrate the ability to explain the history and philosophy of forensic science.

Forensic Science. The student will demonstrate the ability to explain the history and philosophy of forensic science. Forensic Science UNIT I: Introduction to Forensic Science and Human Body The student will demonstrate the ability to explain the history and philosophy of forensic science. a. Define forensic science or

More information

GENETICS CRIME SCENE DNA COLLECTION AND PRESERVATION FOR HUMAN IDENTIFICATION. Innovating Together. copanflock.com

GENETICS CRIME SCENE DNA COLLECTION AND PRESERVATION FOR HUMAN IDENTIFICATION. Innovating Together. copanflock.com GENETICS CRIME SCENE DNA COLLECTION AND PRESERVATION FOR HUMAN IDENTIFICATION Innovating Together copanflock.com A FULLY INTEGRATED COMPANY COPAN S MISSION IS TO IMPROVE THE QUALITY OF THE PRE-ANALYTICAL

More information

DNA & CRIME VICTIMS: WHAT VICTIMS NEED TO KNOW

DNA & CRIME VICTIMS: WHAT VICTIMS NEED TO KNOW DNA & CRIME VICTIMS: WHAT VICTIMS NEED TO KNOW DNA & CRIME VICTIMS: What Victims Need to Know The increasing use of DNA evidence in criminal cases gives victims of crime new hope that offenders will be

More information

Computer with GeneMapper ID (version 3.2.1 or most current) software Microsoft Excel, Word Print2PDF software

Computer with GeneMapper ID (version 3.2.1 or most current) software Microsoft Excel, Word Print2PDF software Procedure for GeneMapper ID for Casework 1.0 Purpose-This procedure specifies the steps for performing analysis on DNA samples amplified with AmpFlSTR Identifiler Plus using the GeneMapper ID (GMID) software.

More information

DNA Detection. Chapter 13

DNA Detection. Chapter 13 DNA Detection Chapter 13 Detecting DNA molecules Once you have your DNA separated by size Now you need to be able to visualize the DNA on the gel somehow Original techniques: Radioactive label, silver

More information

Preserving Forensic Evidence. Preserving Forensic Evidence. at Crime Scenes. Preserving Forensic Evidence. Preserving Forensic Evidence

Preserving Forensic Evidence. Preserving Forensic Evidence. at Crime Scenes. Preserving Forensic Evidence. Preserving Forensic Evidence Preserving Forensic Evidence Preserving Forensic Evidence at Crime Scenes Sgt. Steven J. Wohl Special Victims Unit Spokane Police Department Sgt. Mark Griffiths Major Crimes Unit It is critical to preserve

More information

Once your samples are received the only correspondence you will have with The Genetics Company is via the email address you provide.

Once your samples are received the only correspondence you will have with The Genetics Company is via the email address you provide. Anonymous Infidelity DNA Test Instructions Thank you for your interest in an anonymous Infidelity DNA test. The Genetics Company guarantees to maintain your anonymity. This five page document will outline

More information

Course Contents. MS Forensic Chemistry

Course Contents. MS Forensic Chemistry Course Contents MS Forensic Chemistry Year 1 Semester - I Course Code Course Title Credit Hours FCHEM-7105 Introduction to Forensic Science 3 FCHEM-7106 Crime Scene Investigation 3 FCHEM-7107 Chemistry

More information

Forensic Science Lesson 1: CSI Basics

Forensic Science Lesson 1: CSI Basics Forensic Science Lesson 1: CSI Basics What is Forensic Science? Forensic Science is the application of science to law. Locard s Exchange Principle states that with contact between two items there will

More information

Aurora Forensic Sample Clean-up Protocol

Aurora Forensic Sample Clean-up Protocol Aurora Forensic Sample Clean-up Protocol 106-0008-BA-D 2015 Boreal Genomics, Inc. All rights reserved. All trademarks are property of their owners. http://www.borealgenomics.com support@borealgenomics.com

More information

B2 1 Cells, Tissues and Organs

B2 1 Cells, Tissues and Organs B2 Cells, Tissues and Organs 5 minutes 5 marks Page of 7 Q. The diagram shows a bacterium. On the drawing, name the structures labelled A, B, C and D. (Total 4 marks) Q2. (a) The diagrams show cells containing

More information

Forensics of Blood. The criminalist must be prepared to answer the following questions when examining dried blood:

Forensics of Blood. The criminalist must be prepared to answer the following questions when examining dried blood: FORENSIC SEROLOGY Forensics of Blood The criminalist must be prepared to answer the following questions when examining dried blood: (1) Is it blood? (2) From what species did the blood originate? (3) If

More information

VIRGINIA DEPARTMENT OF FORENSIC SCIENCE EVIDENCE HANDLING & LABORATORY CAPABILITIES GUIDE FORENSIC BIOLOGY

VIRGINIA DEPARTMENT OF FORENSIC SCIENCE EVIDENCE HANDLING & LABORATORY CAPABILITIES GUIDE FORENSIC BIOLOGY VIRGINIA DEPARTMENT OF FORENSIC SCIENCE EVIDENCE HANDLING & LABORATORY CAPABILITIES GUIDE FORENSIC BIOLOGY Contact Information If you have any questions concerning the Forensic Biology laboratory examination

More information

A Comparison Study On The New Formula of Bluestar Latent Bloodstain Reagent and its Effects on DNA Typing/Amplification.

A Comparison Study On The New Formula of Bluestar Latent Bloodstain Reagent and its Effects on DNA Typing/Amplification. A Comparison Study On The New Formula of Bluestar Latent Bloodstain Reagent and its Effects on DNA Typing/Amplification. Justin Lautz*, 1 B.S.; Samantha Webb, 2 B.S. 1 Saint Louis University Clinical Laboratory

More information

DNA Analyst Training Laboratory Training Manual Protocol 2.01 Quality Assurance

DNA Analyst Training Laboratory Training Manual Protocol 2.01 Quality Assurance DNA Analyst Training Laboratory Training Manual Protocol 2.01 Quality Assurance This laboratory protocol (or part thereof) has been provided as an example of a laboratory SOP, courtesy of the Illinois

More information

Commonly Used STR Markers

Commonly Used STR Markers Commonly Used STR Markers Repeats Satellites 100 to 1000 bases repeated Minisatellites VNTR variable number tandem repeat 10 to 100 bases repeated Microsatellites STR short tandem repeat 2 to 6 bases repeated

More information

3.1 Cells and cell function

3.1 Cells and cell function BTEC s own resources 3.1 Cells and cell function In this section: P1 How you are made Key terms Tissue a group of similar cells acting together to perform a particular function. Epithelial cells one of

More information

Analysis of Old Biological Samples: A Study on the Feasibility of Obtaining Body Fluid Identification and DNA Typing Results

Analysis of Old Biological Samples: A Study on the Feasibility of Obtaining Body Fluid Identification and DNA Typing Results Analysis of Old Biological Samples: A Study on the Feasibility of Obtaining Body Fluid Identification and DNA Typing Results T. Spear and N. Khoshkebari, California Criminalistics Institute (CCI), Sacramento,

More information

Melissa May. NetBio - Vice President Strategic Planning. Date: 22/10/2013

Melissa May. NetBio - Vice President Strategic Planning. Date: 22/10/2013 Melissa May NetBio - Vice President Strategic Planning Date: 22/10/2013 Heading Fully automated, Field forward Rapid DNA Typing for Military, Intelligence, and Law Enforcement Applications 090413 Requirements:

More information

7.2 Cells: A Look Inside

7.2 Cells: A Look Inside CHAPTER 7 CELL STRUCTURE AND FUNCTION 7.2 Cells: A Look Inside Imagine a factory that makes thousands of cookies a day. Ingredients come into the factory, get mixed and baked, then the cookies are packaged.

More information

Easy Collection and Extraction of BioSamples Ahlstrom GenCollect Ahlstrom GenCollect Color

Easy Collection and Extraction of BioSamples Ahlstrom GenCollect Ahlstrom GenCollect Color Easy Collection and Extraction of BioSamples Ahlstrom GenCollect Ahlstrom GenCollect Color Ahlstrom GenCollect and Ahlstrom GenCollect Color Collection of biosamples COST Storage at ambient temperature

More information

Introduction to Post PCR Cleanup

Introduction to Post PCR Cleanup Matt Kramer Introduction to Post PCR Cleanup Overview Why post PCR amplification cleanup? Enhancing human identity testing Introduction to QIAGEN MinElute post PCR cleanup technologies MinElute as a tool

More information

DNA & Typing (Unit 11)

DNA & Typing (Unit 11) 117 DNA & Typing (Unit 11) DNA, a long, narrow string-like material, determines an individual s eye color, hair color, stature, bone density and many other traits. Recent advancements in DNA technology

More information

MLX BCG Buccal Cell Genomic DNA Extraction Kit. Performance Characteristics

MLX BCG Buccal Cell Genomic DNA Extraction Kit. Performance Characteristics MLX BCG Buccal Cell Genomic DNA Extraction Kit Performance Characteristics Monolythix, Inc. 4720 Calle Carga Camarillo, CA 93012 Tel: (805) 484-8478 monolythix.com Page 2 of 9 MLX BCG Buccal Cell Genomic

More information

Application of Amido Black Mixture for the Development of Blood-based Fingerprints on Human Skin

Application of Amido Black Mixture for the Development of Blood-based Fingerprints on Human Skin Case Report Application of Amido Black Mixture for the Development of Blood-based Fingerprints on Human Skin Rodney Lawley Birmingham Police Department Birmingham, AL Abstract: Amido black is a chemical

More information

Crime Scene Unit Presumptive Testing Procedures. Evidence Collection Division

Crime Scene Unit Presumptive Testing Procedures. Evidence Collection Division Crime Scene Unit Presumptive Testing Procedures Evidence Collection Division PRESUMPTIVE TESTING Various chemical processes and presumptive tests are utilized in crime scene investigations to assist in

More information

Lecture 13: DNA Technology. DNA Sequencing. DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology

Lecture 13: DNA Technology. DNA Sequencing. DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology Lecture 13: DNA Technology DNA Sequencing Genetic Markers - RFLPs polymerase chain reaction (PCR) products of biotechnology DNA Sequencing determine order of nucleotides in a strand of DNA > bases = A,

More information

The College of Forensic Sciences at NAUSS: The pioneer of Forensics in the Arab world

The College of Forensic Sciences at NAUSS: The pioneer of Forensics in the Arab world 12 Arab Journal of Forensic Sciences and Forensic Medicine 2014; Volume 1 Issue (0), 12-16 Naif Arab University for Security Sciences Arab Journal of Forensic Sciences and Forensic Medicine www.nauss.edu.sa

More information

Faculty Disclosure. Ms. Miller has listed no financial interest/arrangement that would be considered a conflict of interest.

Faculty Disclosure. Ms. Miller has listed no financial interest/arrangement that would be considered a conflict of interest. Faculty Disclosure Amanda S. Miller Ms. Miller has listed no financial interest/arrangement that would be considered a conflict of interest. Developed by Michael L. Stone, SCSA Retired OPD Crime Laboratory

More information

CRIME SCENE PROCEDURES III.

CRIME SCENE PROCEDURES III. CRIME SCENE PROCEDURES III. Evidence Collection... 2 Collection Methods... 3 Evidence Marking and Packaging... 4 Establishing the Chain of Custody... 4 Reporting... 6 Evidence Collection Investigators

More information

BEST PRACTICES FOR COLLECTION, PACKAGING, STORAGE, PRESERVATION, AND RETRIEVAL OF BIOLOGICAL EVIDENCE

BEST PRACTICES FOR COLLECTION, PACKAGING, STORAGE, PRESERVATION, AND RETRIEVAL OF BIOLOGICAL EVIDENCE Collection, Packaging, Storage, Preservation, and Retrieval of Biological Evidence Page 1 of 8 BEST PRACTICES FOR COLLECTION, PACKAGING, STORAGE, PRESERVATION, AND RETRIEVAL OF BIOLOGICAL EVIDENCE Definitions

More information

ABO-Rh Blood Typing With Synthetic Blood

ABO-Rh Blood Typing With Synthetic Blood 70-0101 ABO-Rh Blood Typing With Synthetic Blood Teacher s Manual World-Class Support for Science & Math The ability to type blood is an invaluable tool in the fields of medicine and criminology. Using

More information

Dudesville: A crime scene under the microscope. QUT Extreme Science

Dudesville: A crime scene under the microscope. QUT Extreme Science Dudesville: A crime scene under the microscope QUT Extreme Science Glossary QUT Extreme Science DNA Chromatography Forensic Science Locard s Principle (Deoxyribonucleic acid) an extremely long macromolecule

More information

Forensic Science TEKS/LINKS Student Objectives One Credit

Forensic Science TEKS/LINKS Student Objectives One Credit First Six Weeks Intro/Observation FS 4(A) The student will distinguish between forensic science and criminalistics in law, public safety, corrections, and security. FS 5(D) The student will apply knowledge

More information

Forensic Science : Course Syllabus Forensic Science : Secrets of the Dead

Forensic Science : Course Syllabus Forensic Science : Secrets of the Dead Forensic Science : Course Syllabus Forensic Science : Secrets of the Dead COURSE DESCRIPTION: Fingerprints. Blood spatter. DNA analysis. The world of law enforcement is increasingly making use of the techniques

More information

The Science Detectives- Murder in a Science Lab

The Science Detectives- Murder in a Science Lab CASE STuDY- Evidence Docket Crime Scene Investigation- Scenario: You and your Crime Scene Investigation Unit arrive on the scene of a crime. A man named Dr. Darren Hobbs was found lying on the floor of

More information

FORENSIC BIOLOGY PROTOCOLS FOR FORENSIC STR ANALYSIS Approving Authority: Eugene Y. Lien, Technical Leader Nuclear DNA Operations

FORENSIC BIOLOGY PROTOCOLS FOR FORENSIC STR ANALYSIS Approving Authority: Eugene Y. Lien, Technical Leader Nuclear DNA Operations FORENSIC BIOLOGY PROTOCOLS FOR FORENSIC STR ANALYSIS Approving Authority: Eugene Y. Lien, Technical Leader Nuclear DNA Operations Working version as of 08/14/2015 Highlighted sections indicate a new revision

More information

Chain of evidence refers to the continuity of custody of material and items collected as evidence.

Chain of evidence refers to the continuity of custody of material and items collected as evidence. University of Wisconsin Madison Police Policy: 83.2 SUBJECT: EVIDENCE PROCESSING EFFECTIVE DATE: 06/01/10 REVISED DATE: 12/31/11, 11/01/13 REVIEWED DATE: 01/07/15 INDEX: 83.2.1 COLLECTING, PROCESSING,

More information

06-6542 n/a MYSTERY OF THE BLOOD STAIN (RE)

06-6542 n/a MYSTERY OF THE BLOOD STAIN (RE) DATA SHEET Order code Manufacturer code Description 06-6542 n/a MYSTERY OF THE BLOOD STAIN (RE) The enclosed information is believed to be correct, Information may change without notice due to product

More information

Biotechnology CTY Course Syllabus

Biotechnology CTY Course Syllabus Biotechnology CTY Course Syllabus Day 1, Monday Introduction Pre-Assessment Introductory Topics Ω What is Biotechnology? Ω History of Biotechnology Ω The Scientific Method Ω Prokaryotes vs. Eukaryotes

More information

Proper use of DNA (deoxyribonucleic

Proper use of DNA (deoxyribonucleic JOURNAL ISSUE NO. 249 / JULY 2003 DNA Evidence: What Law Enforcement Officers Should Know Proper use of DNA (deoxyribonucleic acid) evidence at trial can help to seal a conviction or obtain an acquittal.

More information

Pre-Lab Questions. 1. What is cell theory? 2. What do all cells contain? 3. What is a prokaryote? 4. What is a eukaryote? 5. What is an organelle?

Pre-Lab Questions. 1. What is cell theory? 2. What do all cells contain? 3. What is a prokaryote? 4. What is a eukaryote? 5. What is an organelle? Name: TOC# Background Ever since the first microscope was used, biologists have been interested in studying the cellular organization of all living things. After hundred s of years of observations by many

More information

Chapter 2 Forensic Biology: Serology and DNA

Chapter 2 Forensic Biology: Serology and DNA Chapter 2 Forensic Biology: Serology and DNA Lisa Gefrides, MS, FABC and Katie Welch, MS, FABC Contents 2.1 Introduction........................................................... 2.2 Types of Evidence

More information

(A) demonstrate safe practices during laboratory and field investigations

(A) demonstrate safe practices during laboratory and field investigations (1) The student, for at least 40% of instructional time, conducts laboratory and field investigations using safe, environmentally appropriate, and ethical practices. (A) demonstrate safe practices during

More information

Touch DNA: Forensic Collection and Application to Investigations

Touch DNA: Forensic Collection and Application to Investigations Article Original Article Touch DNA: Forensic Collection and Application to Investigations Angela L. Williamson, PhD Bode Technology, Lorton, Virginia, USA Abstract Touch DNA refers to the DNA that is left

More information

Lecture 38: DNA Fingerprinting

Lecture 38: DNA Fingerprinting Lecture 38: DNA Fingerprinting (DNA technology) The most awesome and powerful tool acquired by man since the splitting of atoms - The Time Magazine (USA) Conventional fingerprint of an individual comes

More information

Explain the role of blood and bloodstain patterns in forensics science. Analyze and identify bloodstain patterns by performing bloodstain analysis

Explain the role of blood and bloodstain patterns in forensics science. Analyze and identify bloodstain patterns by performing bloodstain analysis Lab 4 Blood Learning Objectives Explain the role of blood and bloodstain patterns in forensics science Analyze and identify bloodstain patterns by performing bloodstain analysis Introduction Blood, a

More information

Validation Guide for the DNA IQ Reference Sample Kit for Maxwell 16 Printed in USA. 9/06 Part# GE181

Validation Guide for the DNA IQ Reference Sample Kit for Maxwell 16 Printed in USA. 9/06 Part# GE181 REFERENCE MANUAL Validation Guide for the DNA IQ Reference Sample Kit for Maxwell 16 9/06 Validation Guide for the DNA IQ Reference Sample Kit for Maxwell 16 All technical literature is available on the

More information

DNA. Crime Scene and DNA Basics for Forensic Analysts

DNA. Crime Scene and DNA Basics for Forensic Analysts Crime Scene and DNA Basics for Forensic Analysts This course is provided free of charge and is part of a series designed to teach about DNA and forensic DNA use and analysis. Find this course live, online

More information

Today you will extract DNA from some of your cells and learn more about DNA. Extracting DNA from Your Cells

Today you will extract DNA from some of your cells and learn more about DNA. Extracting DNA from Your Cells DNA Based on and adapted from the Genetic Science Learning Center s How to Extract DNA from Any Living Thing (http://learn.genetics.utah.edu/units/activities/extraction/) and BioRad s Genes in a bottle

More information

MODERN METHODS OF COLLECTION AND PRESERVATION OF BIOLOGICAL EVIDENCE FOR HUMAN IDENTIFICATION BY DNA ANALYSIS

MODERN METHODS OF COLLECTION AND PRESERVATION OF BIOLOGICAL EVIDENCE FOR HUMAN IDENTIFICATION BY DNA ANALYSIS MODERN METHODS OF COLLECTION AND PRESERVATION OF BIOLOGICAL EVIDENCE FOR HUMAN IDENTIFICATION BY DNA ANALYSIS Authors: Marian Cătălin (*), Anghel Andrei (*), Oana Mitraşca (*) Abstract: The initial stages

More information

CURRICULUM VITAE Blaine M. Kern

CURRICULUM VITAE Blaine M. Kern CURRICULUM VITAE Blaine M. Kern President/Laboratory Director Human Identification Technologies P.O. Box 608 Redlands, CA 92373-0201 Office: 909-557-1828 Fax: 909-557-1831 E-mail: b.kern@hitdna.com Updated

More information

DNA 1 2. I. Extracting DNA from Your Cells

DNA 1 2. I. Extracting DNA from Your Cells DNA 1 2 I. Extracting DNA from Your Cells Each cell in your body has a nucleus with multiple chromosomes. Each chromosome contains a DNA molecule. Each cell is surrounded by a cell membrane that regulates

More information

UltraClean Forensic DNA Isolation Kit (Single Prep Format)

UltraClean Forensic DNA Isolation Kit (Single Prep Format) UltraClean Forensic DNA Isolation Kit (Single Prep Format) Catalog No. Quantity 14000-10 10 preps 14000-S 1 prep Instruction Manual Please recycle Version: 10302012 1 Table of Contents Introduction...

More information

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to

More information

Forensic sampling and DNA databases: background/issues paper

Forensic sampling and DNA databases: background/issues paper Forensic sampling and DNA databases: background/issues paper Commissioned by: Victorian Parliament Law Reform Committee Written by: Dr Peta Stringer (Manager, DNA Science Branch) Victoria Forensic Science

More information

DNA & CRIME VICTIMS: WHAT VICTIM ASSISTANCE PROFESSIONALS NEED TO KNOW

DNA & CRIME VICTIMS: WHAT VICTIM ASSISTANCE PROFESSIONALS NEED TO KNOW DNA & CRIME VICTIMS: WHAT VICTIM ASSISTANCE PROFESSIONALS NEED TO KNOW What Victim Assistance Professionals Need to Know 1 DNA & CRIME VICTIMS: What Victim Assistance Professionals Need to Know As the

More information

Cells. Introduction WSBCTC 1

Cells. Introduction WSBCTC 1 Cells Cells are the fundamental unit of life. All living things are composed of cells. While there are several characteristics that are common to all cells, such as the presence of a cell membrane, cytoplasm,

More information

Laboratory Examinations, Methods and Procedures. All MN BCA Forensic Science Service (MN BCA FSS) Personnel and Customers

Laboratory Examinations, Methods and Procedures. All MN BCA Forensic Science Service (MN BCA FSS) Personnel and Customers Page 1 of 6 Pages Version: B Issue Date: 02/04/2014 Subject: To: Purpose: Laboratory Examinations, Methods and Procedures All (MN BCA FSS) Personnel and Customers To provide a list of examinations, methods

More information

Supported by. A seven part series exploring the fantastic world of science.

Supported by. A seven part series exploring the fantastic world of science. Supported by A seven part series exploring the fantastic world of science. Find out what techniques are used by forensic scientists and why they are so useful. Forensic science is the term given to the

More information

Blood Stain Analysis Part One

Blood Stain Analysis Part One Hughes Undergraduate Biological Science Education Initiative HHMI Blood Stain Analysis Part One Investigators often find blood stains during their examination of a crime scene. They also find stains that

More information

AmpFlSTR Identifiler Direct PCR Amplification Kit

AmpFlSTR Identifiler Direct PCR Amplification Kit USER GUIDE AmpFlSTR Identifiler Direct PCR Amplification Kit for use with: 200 reaction kit (Part no. 4467831) 1000 reaction kit (Part no. 4408580) Publication Part Number 4415125 Rev. J For Forensic or

More information

The AutoMate Express Forensic DNA Extraction System. Brad Dixon Forensic Science Applications Group

The AutoMate Express Forensic DNA Extraction System. Brad Dixon Forensic Science Applications Group The AutoMate Express Forensic DNA Extraction System Brad Dixon Forensic Science Applications Group Presentation Overview Chemistry and Instrument Overview PrepFiler Express & PrepFiler Express BTA Forensic

More information

CURRICULUM GUIDE. When this Forensics course has been completed successfully, students should be able to:

CURRICULUM GUIDE. When this Forensics course has been completed successfully, students should be able to: CURRICULUM GUIDE NAME OF COURSE: FORENSICS COURSE NUMBER: SCI 40 WRITTEN / REVISED: SEPTEMBER, 2011 LEVEL OF COURSE: REPLACMENT NUMBER OF CREDITS: SIX (6) PREREQUISITES: BIOLOGY GRADE LEVELS OFFERED TO:

More information

DNA Fingerprinting. Biotechnology - Electrophoresis & DNA Fingerprinting Biology 100 - Concepts of Biology 8.1. Name Instructor Lab Section.

DNA Fingerprinting. Biotechnology - Electrophoresis & DNA Fingerprinting Biology 100 - Concepts of Biology 8.1. Name Instructor Lab Section. Biotechnology - Electrophoresis & DNA Fingerprinting Biology 100 - Concepts of Biology 8.1 Name Instructor Lab Section Objectives: To gain a better understanding of: Fundamental Biotechnology Techniques

More information

Plant and Animal Cells

Plant and Animal Cells Plant and Animal Cells a. Explain that cells take in nutrients in order to grow, divide and to make needed materials. S7L2a b. Relate cell structures (cell membrane, nucleus, cytoplasm, chloroplasts, and

More information

Total Test Questions: 71 Levels: Grades 10-12 Units of Credit: 1.0 STANDARD 1 STUDENTS WILL INVESTIGATE THE PAST, PRESENT, AND FUTURE APPLICATIONS OF

Total Test Questions: 71 Levels: Grades 10-12 Units of Credit: 1.0 STANDARD 1 STUDENTS WILL INVESTIGATE THE PAST, PRESENT, AND FUTURE APPLICATIONS OF DESCRIPTION Biotechnology is designed to create an awareness of career possibilities in the field of biotechnology. Students are introduced to diagnostic and therapeutic laboratory procedures that support

More information

1. Blood-screen & confirm 2. Semen screen and confirmation 3. Saliva-amylase 4. Urine-creatinine. 1. Debris examination (macroscopic) 2. GC 3.

1. Blood-screen & confirm 2. Semen screen and confirmation 3. Saliva-amylase 4. Urine-creatinine. 1. Debris examination (macroscopic) 2. GC 3. TYPE AND EXTENT OF EXAMINATIONS OFFERED BY THE DSS LABORATORIES The Division of Scientific Services Laboratories offer forensic analysis of materials in the general areas of Criminalistics, Identification,,

More information

Forensic science ( 科 學 鑑 證 ):

Forensic science ( 科 學 鑑 證 ): Reference for slides 3 20: Forensic Science ( 科 學 鑑 證 ) Criminalistics An Introduction to Forensic Sciences, 7th ed. By Richard Saferstein Prentice Hall, NJ, c2001. IJS Training (Phase 3) Dr. Kendrew K.

More information

Chicken or the Egg: Process for Latent Prints or DNA First? Karley Hujet Diana Tabor

Chicken or the Egg: Process for Latent Prints or DNA First? Karley Hujet Diana Tabor Chicken or the Egg: Process for Latent Prints or DNA First? Karley Hujet Diana Tabor Certified Latent Print Examiners Wisconsin State Crime Laboratory - Madison Background At the time of the project, the

More information

DNA Fingerprinting. Unless they are identical twins, individuals have unique DNA

DNA Fingerprinting. Unless they are identical twins, individuals have unique DNA DNA Fingerprinting Unless they are identical twins, individuals have unique DNA DNA fingerprinting The name used for the unambiguous identifying technique that takes advantage of differences in DNA sequence

More information

ABSTRACT. Promega Corporation, Updated September 2008. http://www.promega.com/pubhub. 1 Campbell-Staton, S.

ABSTRACT. Promega Corporation, Updated September 2008. http://www.promega.com/pubhub. 1 Campbell-Staton, S. A Modified Wizard SV Genomic DNA Purification System Protocol to Purify Genomic DNA... A Modified Wizard SV Genomic DNA Purification System Protocol to Purify Genomic DNA from Shed Reptile Skin ABSTRACT

More information

Introduction to the Cell: Plant and Animal Cells

Introduction to the Cell: Plant and Animal Cells Introduction to the Cell: Plant and Animal Cells Tissues, Organs, and Systems of Living Things Cells, Cell Division, and Animal Systems and Plant Systems Cell Specialization Human Systems All organisms

More information

DNA Stability Studies: FTA vs 903

DNA Stability Studies: FTA vs 903 Forensics @ NIST Gaithersburg, MD DNA Stability Studies: FTA vs 93 Margaret C. Kline Overview History of DNA storage studies at NIST Stability at different temperatures and different papers Review Anal

More information

Discovering cells. The fi rst drawing of cells

Discovering cells. The fi rst drawing of cells Discovering cells Living things are made of cells. Just as bricks and planks of wood are the building blocks for houses, cells are the building blocks of living things. Cells, although they are very small,

More information

Application Guide... 2

Application Guide... 2 Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue Application Guide... 2 I. Description... 2 II. Product Components... 2 III. Materials to be Supplied

More information

The Cell. Grade 8 Activity Plan

The Cell. Grade 8 Activity Plan The Cell Grade 8 Activity Plan Plant Cell Project Objectives: 1. To identify cell organelles and their functions. 2. To demonstrate the difference between plant and animal cells. Keywords/concepts: cells,

More information