Appendix 2 Molecular Biology Core Curriculum. Websites and Other Resources
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1 Appendix 2 Molecular Biology Core Curriculum Websites and Other Resources Chapter 1 - The Molecular Basis of Cancer 1. Inside Cancer From the Dolan DNA Learning Center Cold Spring Harbor Laboratory Chapter 2 Molecular Science Animation of PCR cycles Gel Electrophoresis 4. outhern%20blot Southern Blot DNA to Protein (transcription) Molecules of Genetics: Concepts, animation, and problems for each unit, depending on students' previous background 7. Cell Biology and Molecular Biology Hologic website Download text and view videos and descriptions of each of the following: replication, transcription, translation, DNA, RNA, codon, mrna, trna, enzymes, chromosomal abnormalities. Some animations such as "Animated tour Through the Cell", for example are also available in these 2 sections Dolan DNA Learning Center DNA from the Beginning: Classical Genetics, Molecules of Genetics, Genetic Organization and Control In addition to the topics listed below, this site has a number of other useful sections, as well. Click on the "websites" tab for several online tutorials. See Also Biology Animations: These may be inserted into the PowerPoints or used for other purposes as you move through the curriculum. Cloning 101 Cycle Sequencing DNA Arrays DNA Restriction DNA Transformation 1 DNA Transformation 2 Gel Electrophoresis GeneChip How Alu Jumps Model Organisms Polymerase Chain Reaction Sanger Sequencing Stem Cell Lines 3-D Animations: DNA Molecule: DNA Has Four Units
2 Chargaff's Ratios Triple Helix Basepairing How DNA is Packaged (Basic) How DNA is Packaged (Advanced) DNA Unzip Chromosome Map How Much DNA Codes for Protein? Chromosome 11 Flyover Transcription & Translation: Triplet Code Transcription (Basic) Transcription (Advanced) mrna splicing Translation (Basic) Translation (Advanced) Replication: Replicating the Helix Mechanism of Replication (Basic) Mechanism of Replication (Advanced) Experiments & Techniques: Mechanism of Recombination Microarray Polymerase Chain Reaction Sanger Sequencing Public Project Sequencing Private Project Sequencing Disease & Mutation: DNA Damage Sickle Cell Tumor Growth How Gleevec Works Cell Signals 9. Another great source for some good basic information. See Tour the Basics DNA, Gene, Chromosome, Protein, Heredity, Traits DNA to Protein Protein synthesis
3 Heredity and Traits Genetics Amazing Cells Basic on cells Stem Cells Cloning Gene Therapy Transgenic Mice Virtual Labs on: DNA Extraction, Gel Electrophoresis, PCR, and DNA Microarray The Archive has some basic information on mutations and how they occur. See also the teachers' site at which has some great information on epigenetics. The page on "Lick your Rats" is fun and good to explain epigenetics (Scroll down to the bottom of the page; page updated as of 5/21/2010). Roche Genetics CD, Introduction and Journey through the Cell. This website gives information on obtaining this CD from Roche. There are also several others: Molecular Technology and Regulatory. There is a Teacher Manual for the Roche Genetics CD available online as well In Dolan DNA Learning Center, see Flyover a close look at chromosome 11, and Chromosome Close Up which contains a 3-D animation of chromosome coiling from DNA to nucleosome to mitosis. It also includes information on X chromosome banding areas, a 2-D animation including explanations of exons and introns. Genome mining includes information about transcription, 3-D animation of promotors, TATA boxes, etc. Genome FISHing has a video regarding variability in the genome such as SNPs and RLFPs. Genome Spots compares various areas of chromosomes as to their variability, genes encoded, etc cell cycle DNA replication Chapter 3 Molecular Techniques Molecular Methods of Separation and Detection 16. Actual Lab: Website: The extraction of DNA from anything living. A kitchen level actual lab which shows the principles of DNA extraction. 17. Virtual Lab: DNA Extraction. 18. Hologic website Instrumentation Electrophoresis Lab, ISH Lab, Amplification Lab. 19. Resource: Roche CD, Molecular Technology: Microarrays, in situ Hybridization, Agarose Gel Electrophoreses, SDS-PAGE, Southern, Northern, and Western Blots, Troubleshooting 20. Actual Labs: Colorful Electrophoresis, Build a Gel Electrophoresis Chamber.
4 21. Virtual Lab: Gel Electrophoresis Resources: Roche CD, Polymerase Chain Reaction: Order CD and use PCSEC1.ppt (DNA to PCR), PCSEC2.ppt (In the test tube), PCSEC3.ppt (Minimal requirements), PCSEC4.ppt (Machines used in the process), Glossary important terms. {Note: this CD may no longer be available from Roche contact company for latest update} Molecular Methods Amplification: PCR through LCR. 24. Virtual Lab: PCR Molecular Methods - DNA sequence analysis: pyrosequencing through automated sequence analysis Dolan DNA Learning Center DNA Interactive Code. Manipulation, Techniques: Amplifying Making many copies 2-D and Amplifying PCR 3-D Cutting and Pasting Restriction enzymes 2-D and Recombining DNA 3-D animation Transferring and Storing DNA Transformation 2-D animation, Storing DNA 2-D animation Large Scale Analysis - GeneChips 2-D animation, Making GeneChips Slideshow, DNA arrays 2-D Sorting and Sequencing Gel 2-D, Early DNA Sequencing 2-D, Cycle Sequencing 2-D Northern blot Pyrosequencing RFLP VNTR Chapter 4 Laboratory Operations Regulations and Licensure Links to state regulations and definitions of terms. Chapter 5 Molecular Technology Applications Pathogens Dolan DNA Learning Center Molecular Applications: Human Identification, Family Identification, Murder (forensics), Innocence (forensics) How Gleevec works. Animation in Dolan Learning Center Genetics and Health, Personalized Medicine: a. Your Doctor's New Genetic Tools, b. Making SNPs Make Sense, c. Drug Development Today and Tomorrow. 3 modules which talk about genetics and drug development. The interactive frog module is kind of fun also but may be too "young" for our students. Chapter 6 - FISH Chapter 7 Circulating Tumor Cells
5 36. Video: -CellSearch General References: Hologic Website Summary: Molecular Although this site is referenced frequently in the appropriate places in each chapter, a summary of the various areas follows for ease of use by students and educators: Cell Biology Molecular Biology Biology of Infections Molecular Methods Instrumentation Regulations and Licensure
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