VALIDATED MOLECULAR SOLUTIONS FOR CYTOGENETICISTS. PRODUCTS AND METHODOLOGIES Not for distribution in the USA

Transcription

1 PRODUCTS AND METHODOLOGIES Not for distribution in the USA VALIDATED SOLUTIONS FOR MOLECULAR CYTOGENETICISTS

2 2

3 A GLOBAL LEADER IN HUMAN AND ENVIRONMENTAL HEALTH PerkinElmer is a global scientific leader that is taking action to improve the health and safety of people and their environment. We are committed to protecting the health of expectant mothers, babies and families. PerkinElmer is the world s leading supplier of newborn screening systems, a pioneer in methods for risk assessment during pregnancy and a leader in cord blood banking. Following its acquisition by PerkinElmer in 2010, Signature Genomics has enhanced PerkinElmer s molecular cytogenetics expertise. SIGNATURE GENOMICS - CYTOGENETICS PIONEER Signature Genomics has established cutting-edge methodologies that are now standard in molecular cytogenetics. Founded in 2003 Signature Genomics has analyzed over 50,000 cytogenetic samples and established a powerful database of abnormalities. Based on this information a unique acgh array design has been defined that targets relevant regions for molecular cytogenetic analysis. As one of the pioneers in the field of molecular cytogenetic analysis, we continuously work towards innovation and development of cutting-edge technologies to improve human health. We aim to share our experience and expertise with the global cytogenetics community and significantly advance the field of cytogenetics. 3

4 PerkinElmer is working with its sizeable customer base (in the regions marked blue on the map) to advance health care. PERKINELMER - INNOVATORS IN MOLECULAR CYTOGENETICS Faster results higher sensitivity PerkinElmer is bringing the newest molecular techniques to cytogenetic laboratories. Our innovative methodologies support: Higher detection rates More results - faster & cheaper Fewer unclear results Streamlined processes PerkinElmer support from sample to result Oligonucleotide-based microarrays and bead-based multiplex BACs-on-Beads (BoBs) products have been specifically designed, developed and validated by cytogeneticists for cytogeneticists performing molecular karyotyping. With the recent acquisition of chemagen, PerkinElmer is now able to support the entire workflow incorporating automated DNA/RNA isolation solutions. 4

5 PRODUCTS AND METHODOLOGIES CONTENTS Products for Research chemagen Technology - DNA/RNA extraction and purification...6 EasyAmp - Single cell whole genome amplification...8 BACs-on-Beads - Power to expand on existing FISH capabilities...9 KaryoLite BoBs...11 Array CGH - A comprehensive product portfolio... CGX Oligo Arrays...13 CGX Hybridization Oven...14 ScanRI Scanner...15 Genoglyphix visualization software and database...16 Products for IVD Prenatal BoBs

6 DNA/RNA ISOLATION UNIQUE TECHNOLOGY FOR DNA/RNA ISOLATION chemagen Technology Complete Solutions for DNA/RNA Isolation in Human Genetics A crucial step in human genetic testing is the isolation of high quality DNA or RNA. PerkinElmer s chemagen Technology offers flexible solutions for applications in: Biobanking Human Genetics Epigenetics CHEMAGIC MSM I OUTSTANDING FLEXIBILITY 10 μl - 10 ml samples with one instrument Wide variety of sample material (Blood, Blood Spots, Plasma, Cells, Tissue) Easy to connect to standard liquid handling systems As the number of samples increases, the need for automation becomes more acute. Automation also offers better reproducibility and reliability in the isolation process. The key competence behind chemagen Technology is the isolation of genomic DNA and total RNA. This competence is expressed in chemagic Kit products, which contain proprietary magnetic particles with a high affinity to nucleic acids and low protein binding. CHEMAGIC PREPITO INNOVATIVE BENCHTOP SOLUTION Up to 1 ml samples, 1 - samples in parallel Pre-installed protocols Cost-effective sample preparation CHEMAGIC KITS - MAGNETIC BEADS FOR ENDLESS POSSIBILITIES All products listed are for Research Use Only. Not for use in diagnostic procedures. Several kits for the isolation of genomic DNA and total RNA Suitable for use with liquid handling instruments and automated magnetic bead-based platforms Available as manual kits 6

7 DNA/RNA ISOLATION CHEMAGIC KITS OVERVIEW The chemagic Kit portfolio comprises a large number of kits for the isolation of DNA and RNA. Human Genetics projects and Biobanking Instrument Sample Volumes Samples/Run chemagic DNA Blood Kit special 50 µl µl blood 1 ml - 4 ml blood 3 ml - 10 ml blood chemagic DNA Blood Spot Kit special Paper filter punch outs 96 chemagic DNA Saliva Kit special 4 ml saliva 24 chemagic DNA Buccal Swab Kit special 1 swab 96 chemagic DNA CellM Kit special 1.2 x 10 7 cells Prepito DNA Blood250 Kit chemagic Prepito 100 µl µl Prepito DNA Blood600 Kit chemagic Prepito 600 µl 6 Prepito FFPE Kit chemagic Prepito 10 µm section Prepito DNA Tissue Kit chemagic Prepito animal tissues or cells chemagic SEQ Pure Kit Manual or on LH instruments 5 µl - 20 µl (without ethanol) Prenatal Genetics and Epigenetics Instrument Sample Volumes Samples/Run chemagic Circulating NA Kit Special 1 ml serum/plasma 4 ml serum/plasma 24 chemagic Amniotic Fluid Kit special 1 ml amniotic fluid chemagic Epigenetic NA Extraction Kit special 5 ml plasma 4 ml plasma chemagic Epigenetic Bisulfite Purification Kit special up to 500 µl bisulfite reaction mix 96 Prepito DNA Cyto Pure Kit chemagic Prepito 250 µl blood, 10 mg tissue or 1-5 ml pelleted amniotic fluid Prepito Circulating NA1k Kit chemagic Prepito 1 ml serum/plasma Isolation of total RNA Instrument Sample Volumes Samples/Run chemagic RNA Blood Kit special 2.5 ml stabilized blood 2.5 ml stabilized blood 24 chemagic RNA Saliva Kit special 1 ml serum/plasma chemagic mrna/gdna Kit special 2 ml blood or bone marrow; max. cell number 20 x 10 6 Further chemagic Kits are available on request. Products listed are for Research Use Only. Not for use in diagnostic procedures. 7

8 WHOLE GENOME AMPLIFICATION EASYAMP Single Cell Whole Genome Amplification with EasyAmp EasyAmp is a single cell whole genome amplification (WGA) kit that can be used when the amount of DNA is not sufficient to perform the direct analysis, e.g. research in vitro fertilization (IVF) applications on single cells. EasyAmp WGA is based on technology from Rubicon Genomics. Reliable and Reproducible Amplification Technology EasyAmp enables the researcher to obtain the amount of DNA from only one cell that previously required over 10,000 cells for subsequent analyses. EasyAmp, in contrast to other single cell amplification methods, delivers amplified genomic DNA that reproducibly represents all sequences with low allele drop out. EasyAmp can be used on polar bodies, blastomere or blastocyst cells for preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS) research applications. RAPID AND ROBUST ONE-TUBE PROTOCOL Simple 1-tube, 3-step and 3 hour protocol Eight components sufficient to perform 50 (or ) reactions About 1 million fold amplification of a single cell to produce 3-5 micrograms of DNA Low background Reproducible locus amplification No allele bias EasyAmp reagents are for Research Use Only. Not for use in diagnostic procedures. Lysis/extraction buffer Pre-amp cocktail Amplification cocktail Add cell in ~2 microliters or less PBS 15 min 90 min 60 min Analysis 3 µg adna The EasyAmp whole genome amplification process amplifies a single cell s DNA approximately one million-fold to give 3-5 micrograms of amplified DNA. 8

9 BACS-ON-BEADS TECHNOLOGY THE POWER OF BACS-ON-BEADS BACs-on-Beads (BoBs ) is an exciting new technology from PerkinElmer. By immobilizing BAC (bacterial artificial chromosome) derived DNA probes onto Luminex xmap fluorescently coded beads, BoBs enables rapid detection of copy number changes in targeted genomic regions from a minute amount of DNA. BACs-on-Beads technology supports high throughput molecular karyotyping in a microplate well which, in turn, can lead to greater laboratory efficiency and better use of resources. Expand on your FISH capability using BoBs technology BACs are large cloned sequences of human DNA, typically 150, ,000 basepairs long. When coupled to Luminex xmap beads, the length of the BACs is a useful property. BAC derived DNA probes have a higher signal-to-noise ratio compared to smaller DNA probes regardless of the DNA quality. This represents an important advantage when working with samples such as amniotic fluid, CVS, POC (product of conception) and DNA amplified from single or few cells. The advantage of BACs over oligonucleotides, which are often less than 100 basepairs long, is that fewer probes are needed for the same coverage of a target region. Results of unclear clinical significance that are common with other fast molecular karyotyping techniques are rarely encountered with the new BACs-on-Beads assays. BAC DNA has long been used as probes for FISH (fluorescence in situ hybridization) in cytogenetic laboratories. Now, with the availability of the BACson-Beads technology multiple FISH probes can be handled in a single well and you can perform the equivalent of tens to hundreds of FISH experiments simultaneously. 9

10 BACS-ON-BEADS TECHNOLOGY LUMINEX XMAP TECHNOLOGY Assay Based on Luminex xmap Technology is an established multiplexing technology utilizing approximately 5 μm diameter polystyrene beads that have been impregnated with a specific ratio of two different fluorescent dyes. By using ten different concentrations of each of the two dyes it is possible to generate up to 100 bead types with distinct fluorescent signatures, or spectral addresses that can be identified through excitation of the impregnated dyes when read by the Luminex 100/200 instrument. The advantage of the Luminex instrument platform lies in the fact that up to 100 different bead types can be mixed together and the experiment can be performed in a multiplexed fashion using microplates. BOBS PRODUCTS BRING THESE BENEFITS TO YOUR WORK Robust assay with low quality DNA from various sample types Enables high throughput analysis as tens of samples can be run simultaneously reducing the hands-on time Resources are optimized as sample volume can be increased without increasing staffing Complete procedure from extracted DNA to result availability takes less than 24 hours Results are clear and easy to interpret More information due to the multiplexing power of xmap The instrument can automatically aspirate the beads from each well of a 96-well plate, and by use of a flow system that allows one bead to be interrogated by two lasers simultaneously, all beads can be classified and quantified within the assay in a short amount of time. Easy interpretation of the Copy Number Changes with BoBsoft Analysis Software BoBsoft analysis software takes the output file generated by the Luminex 100/200 instrument, and analyzes the data. The signal intensities from the sample and reference DNA are compared to provide a clear display of any copy number changes in the targeted regions. 10

11 BACS-ON-BEADS KARYOLITE TECHNOLOGY BOBS KARYOLITE BOBS 24 Chromosomes in 24 Hours KaryoLite BoBs has been developed to detect arm specific aneuploidies in all 24 chromosomes in a single assay for research use only. The product covers p and q arms of all chromosomes 1-22, X and Y. Based on the BACs-on-Beads technology, it consists of BAC DNA immobilized onto polystyrene microspheres distinguishable by the Luminex instrument system. KaryoLite BoBs utilizes a new concept of composite beads having DNA from three different BAC clones on each bead type. The composite clone format expands the region of chromosomal DNA interrogated by each bead. KaryoLite BoBs utilizes a new concept of composite beads having three different BAC clones on each bead type. Research may be performed with KaryoLite BoBs using only 50 ng of genomic DNA extracted directly from amniotic fluid, chorionic villae or fetal tissue. As no cell culturing is required with any BoBs assay, KaryoLite BoBs offers a clear advantage over conventional cytogenetic techniques in cases in which cell culture is problematic, for example in POC (product of conception) analysis. Results in Under 24 hours with Hands-On Time ~ 3.5 hours The probes used have been carefully selected to give information about the whole chromosome including the distal and proximal region of the chromosome arms. For acrocentric chromosomes (13, 14, 15, 21 and 22) three beads have been selected to cover exclusively the q-arms. See these references Grati FR et al. Application of a new molecular technique for the genetic evaluation of products of conception. Prenat Diagn. 20, 32, DOI: /pd Paxton CN et al. Rapid aneusomy detection in products of conception using the KaryoLite BACS-on-Beads assay. Prenat Diagn. 20, 32, 1-7. DOI: /pd KaryoLite BoBs reagents are for Research Use Only. Not for use in diagnostic procedures. 11

12 ARRAY COMPARATIVE GENOMIC HYBRIDIZATION TECHNOLOGY ARRAY CGH Oligonucleotide Array CGH Solution developed by Signature Genomics Traditional karyotyping methods, such as FISH or G-banding, are well established for detecting chromosomal aberrations however, these methods are limited by speed, throughput and resolution. While these traditional methods are able to detect microscopic visible chromosomal alterations such as an extra chromosome band, smaller gains or losses in the genome cannot be found reliably. Array CGH in contrast enables higher resolution genome analysis and allows the detection of submicroscopic chromosomal imbalances across the genome in one single experiment. As one of the pioneers in the field of Molecular Cytogenetics, Signature Genomics, a PerkinElmer company, has early on established cutting-edge technologies that are now the standard for detection of chromosomal abnormalities. Signature Genomics from the beginning has used a genotype-first approach and has developed an oligoarray design for the detection of submicroscopic aberrations associated with learning disability and dysmorphic features. This array design has led to a partnership between Signature Genomics, PerkinElmer and Agilent Technologies, Inc. This partnership allows clinical researchers access to relevant cytogenetic content through the use of our streamlined and robust CGX workflow.

13 ARRAY COMPARATIVE GENOMIC HYBRIDIZATION TECHNOLOGY CGX OLIGO ARRAYS CGX Oligo Arrays are oligonucleotide-based microarrays specifically designed, developed and verified by Signature Genomics for the detection of small genetic aberrations associated with learning disability and dysmorphic features in research applications. The oligonucleotide probes on the CGX, CGX -HD and CGX -SNP arrays cover the entire human genome with specific focus on over 245 cytogenetically relevant regions, 980 functionally significant genes, pericentromeric regions, and subtelomeres. Almost every abnormality detected by CGX Oligo Arrays can be visualized by FISH FISH confirmation might be required to identify structural changes and the possible source of any change. With the possibility for ordering unlabeled BAC clone DNA for confirmation purposes, PerkinElmer offers a total solution package consisting of discovery and confirmation options. CGX Oligo Arrays are Research Use Only products. Not for use in diagnostic procedures. CGX Oligo Arrays are designed by Signature Genomics and manufactured by Agilent Technologies, Inc. CGX OLIGO ARRAYS CHARACTERISTICS THAT WILL BENEFIT YOUR WORK Proven design, representing over 245 known syndromic regions and over 980 functionally significant genes Three different array types based on the same design to adjust for throughput and detection requirements including Absence of Heterozygosity (AOH) and Uniparental Disomy (UPD) detection CGX : 8x60K with a resolution of 190 kb in the backbone, and 28 kb in the targeted regions CGX -HD: 4x180K with a resolution of 40 kb in the backbone, and 20 kb in the targeted regions CGX -SNP: 4x180K with a resolution of 80 kb in the backbone, and 20 kb in the targeted regions. In addition, detection of contiguous stretches of Absence of Heterozygosity (AOH) of approximately 5-10 Mb All designs updated to HG19 13

14 ARRAY COMPARATIVE GENOMIC HYBRIDIZATION TECHNOLOGY CGX HYBRIDIZATION OVEN CGX Hybridization Oven The CGX Hybridization Oven is designed for optimal hybridization performance to achieve consistent and reliable results. The oven is a compact and flexible instrument designed for an optimal microarray processing workflow. High Throughput The oven is designed to hold up to 24 CGX Hybridization Chambers. Consistent Data Reproducible results; increased sensitivity and specificity. Flexibility Variable temperature control range; from + 5 to 70 C (+/- 0.1 C) Variable rotation speed control from 5 to 20 RPM. SPECIFICATIONS Rotator Motor Speed Approximate Oven Dimensions Approximate Chamber Dimensions 2 to 20 RPM Height: 22.0" (55.8 cm) Width: 17.4" (44.5 cm) Depth: 17.75" (39.5 cm) Height: 14.5" (36.8 cm) Width:.5" (31.8 cm) Depth:.0" (30.5 cm) Operating Temperature Range Weight Power Input Hybridization Chamber Rotator Rack + 5 to 70 C (+/- 0.1 C) 75 lbs (34.0 kg) volts, volts Holds up to 24 hybridization chambers during hybridization 14

15 ARRAY COMPARATIVE GENOMIC HYBRIDIZATION TECHNOLOGY SCANNING AND VISUALIZATION Fast and Sensitive Scanning with ScanRI ScanRI is one of the fastest microarray scanners on the market enabling the capture of images in approximately 20 min when scanned at 3 μm resolution. It provides simultaneous 2-color image acquisition with scanning resolution from 3 μm to 40 μm. Combined with its small size, low background noise and high sensitivity, ScanRI provides an attractive solution for array users. SCANRI SCANNER - MAIN SPECIFICATIONS Weight 15.5 kg Dimensions 278 x 457 x 369 mm 3 Laser excitation wavelengths of 532 nm and 635 nm Simultaneous 2-color scanning Scanning resolution from 3 μm to 40 μm Low background noise and high sensitivity Uniform scanning across the microarray slide 15

16 ARRAY COMPARATIVE GENOMIC HYBRIDIZATION TECHNOLOGY PERKINELMER ARRAY SOFTWARE SOLUTIONS Genoglyphix : turning 50,000 samples into meaning Genoglyphix is a powerful data visualization software and database based on the analysis of over 50,000 verified cytogenetic samples. Genoglyphix offers a proven analysis tool with a complete workflow including sample tracking, aberration categorization, data interpretation, report creation functionality and data sharing options with other Genoglyphix users worldwide. DESIGNED BY CYTOGENETICISTS FOR CYTOGENETICISTS Genoglyphix sets a new standard for array based cytogenetic analysis. Developed by Signature Genomics and available exclusively with CGX oligo arrays, Genoglyphix genome browser software provides intuitive data visualization and annotation features for streamlined and rapid analysis of CGX data. Genoglyphix software is for Research Use Only. Not for use in diagnostic procedures. GENOGLYPHIX BRINGING MULTIPLE GENETIC DATABASES INTO ONE TOOL Access to Signature s database containing over,000 verified genetic alterations identified in over 50,000 samples Direct links to other relevant databases such as the DGV, OMIM, PubMed, UCSC and Ensembl for easy data interpretation Secure web based (8-bit encryption) access to Genoglyphix database and software GENOGLYPHIX EASILY GUIDES CYTOGENETICISTS THROUGH ANALYSIS WITH CONVENIENT TOOLS Generation user defined databases and custom tracks displaying abnormal results, copy number variants and analysis notes Data display in HG18 or HG19 Report creation functionality Optional sharing of data with other Genoglyphix users Availability of FISH probes for result confirmation 16

17 ARRAY COMPARATIVE GENOMIC HYBRIDIZATION TECHNOLOGY GENOGLYPHIX GENOME BROWSER GRAPHICAL REPRESENTATION OF ABERRATIONS IN THE GENOGLYPHIX GENOME BROWSER Display of normal copy number variation in DGV within the selected genomic region Display of similar aberrations within the Signature Genomics data base Display of GC content in the selected genomic region Display of genes within the selected genomic region Display of known syndromes within the selected genomic region 17

18 IVD PRODUCT PRENATAL BOBS A more informative option than FISH and QF-PCR for IVD Labs Prenatal BoBs is a CE-marked IVD product based on BACson-Beads technology. In addition to detecting copy number changes of chromosomes 13, 18, 21, X and Y, the product enables detection of 9 additional chromosomal regions in which a clear correlation between a loss and an adverse outcome has been demonstrated. 1 Copy number changes in these targeted microdeletion regions are not easily found with other commonly used methods. Also the microdeletion syndromes detected by Prenatal BoBs often are not inherited and do not display ultrasound abnormalities, so they may otherwise be missed in a prenatal setting. Prenatal BoBs is a CE-marked IVD product based on PerkinElmer BACs-on- Beads technology (described on pages 9-10). Low Input Material Fast Results Analysis using Prenatal BoBs can be performed with ng genomic DNA, an amount which can be obtained from 3-5 ml of amniotic fluid or from 1 villus. Results are ready in hours from the time of sampling. Clear Result Interpretation In Prenatal BoBs a gain or loss of a region is called if three or more probes within a given target region exceed the cut-off for gain or loss. Frequencies of aneuploidies and single microdeletion syndromes vary from common to rare. It is expected that there is a finding of an aneuploidy or microdeletion syndrome region with the Prenatal BoBs kit in every 1/250 births. 2 Prenatal BoBs reagents are not available in the USA and Canada. In other countries please check availability with your PerkinElmer sales representative. Aneuploidies Cytoband Trisomy 13: Patau Syndrome Chr 13 Trisomy 18: Edwards Syndrome Chr 18 Trisomy 21: Down Syndrome Chr 21 Sex Chromosome Abnormalities Chr X, Chr Y Microdeletion Syndrome Cytoband DiGeorge Syndrome 22q11.2 DiGeorge 2 Syndrome 10p14 Williams-Beuren Syndrome 7q11.2 Prader-Willi Syndrome 15q11-q Angelman Syndrome 15q11-q Smith-Magenis Syndrome 17p11.2 Wolf-Hirschhorn Syndrome 4p16.3 Cri du Chat Syndrome 5p15.3-p15.2 Langer-Giedion Syndrome 8q23-q24 Miller-Dieker Syndrome 17p13.3 LITERATURE: 1. OMIM: #105830, #3450, #150230, #176270, #182290, #188400, #194050, #194190, and # Vialard et al. Prenatal BACs-on-Beads: the prospective experience of five prenatal diagnosis laboratories, Prenat Diagn. 20, 32, See also Cheng YKY et al. The detection of mosaicism by Prenatal BoBs Prenat Diagn. 20, 32, 1 8 DOI: /pd Gross SJ et al. Rapid and novel prenatal molecular assay for detecting aneuploidies and microdeletion syndromes, Prenat Diagn Mar;31(3): Vialard F et al. Prenatal BACs-on-Beads: a new technology for rapid detection of aneuploidies and microdeletions in prenatal diagnosis, Prenat Diagn May;31(5): Shaffer LG et al. The development of a rapid assay for prenatal testing of common aneuploidies and microdeletion syndromes, Prenat Diagn Aug;31(8): Popowski T et al. Williams-Beuren Syndrome: the prenatal phenotype, Am. J. Obs & Gyn Izzo A et al. 40 Mb duplication in chromosome band 5p13.1p15.33 with 800 kb terminal deletion in a foetus with mild phenotypic features, Eur. J. Med. Genet. 20 Feb 55 (2)

19 ORDERING INFORMATION Product Number Product Name Product Number Product Name chemagen products Easy Amp CMG-1072 chemagic DNA Blood Kit special, 50 µl, 96 samples EasyAmp 50 reaction WGA kit CMG-721 chemagic DNA Blood Kit special, 100 µl, 96 samples EasyAmp reaction WGA kit CMG-746 chemagic DNA Blood Kit special, 250 µl, 96 samples BACs-on-Beads CMG-1091 chemagic DNA Blood Kit special, 400 µl, 96 samples Luminex 200 w/ xponent CMG-1086 chemagic DNA Blood Kit special, 1 ml, 24 samples Constitutional BoBs CMG-1097 chemagic DNA Blood Kit special, 2 ml, 24 samples KaryoLite BoBs CMG-1074 chemagic DNA Blood Kit special, 4 ml, 24 samples BoBsoft 1.1 Research Analysis Software CMG chemagic DNA Blood Kit special (4 ml elution tubes), 3 ml, samples BoBsoft 2.0 Research Analysis Software CMG chemagic DNA Blood Kit special (13 ml elution tubes), 3 ml, samples CGX CMG chemagic DNA Blood Kit special (13 ml elution tubes), 5 ml, samples ScanRI CMG chemagic DNA Blood Kit special (4 ml elution tubes), 5 ml, samples ScanRI PC CMG-715 chemagic DNA Blood Kit special, 7 ml, samples Genoglyphix SW CMG-704 chemagic DNA Blood Kit special, 10 ml, samples CGX HD/SNP Gasket Slides-100 CMG-1030 CMG-1081 CMG-1035 CMG-748 CMG-756 CMG-2002 CMG-2004 CMG-2027 CMG-2010 CMG-108 CMG-459 CMG-458 CMG-1096 CMG-1090 CMG-797 CMG-1068 CMG-1088 CMG-1069 CMG-2034 chemagic DNA Blood Spot Kit special, 3-6 mm paperfilter punch outs, 96 samples chemagic DNA Saliva Kit special, 4 ml, 24 samples chemagic DNA Saliva Kit special, samples chemagic DNA Buccal Swab Kit special, 96 samples chemagic DNA CellM Kit special, samples Prepito DNA Blood250 Kit, samples Prepito DNA Blood600 Kit, 6 samples Prepito FFPE Kit, 10 µm section, samples Prepito DNA Tissue10 Kit, 10 mg tissue, samples chemagic SEQ Pure Kit, µl chemagic SEQ Pure Kit LH, 10 µl chemagic SEQ Pure Kit LH, 20 µl chemagic Circulating NA Kit special, 1 ml, samples chemagic Circulating NA Kit special, 4 ml, 24 samples chemagic DNA Amniotic Fluid Kit special, 1 ml, samples chemagic Epigenetic NA Extraction Kit special, 5 ml, samples chemagic Epigenetic NA Extraction Kit special, 4 ml, 24 samples chemagic Epigenetic Bisulfite Purification Kit special, 500 µl, 96 samples Prepito DNA Cyto Pure Kit, samples P CGX Gasket Slides-100 CGX HD/SNP Gasket Slides-20 CGX Gasket Slides-20 CGX Ozone-barrier slide covers CGX (2 slides per pack) CGX (4 slides per pack) CGX HD (2 slides per pack) CGX HD (4 slides per pack) CGX SNP (2 slides per pack) CGX SNP (4 slides per pack) CGX Hybridization chamber, stainless CGX Hybridization oven CGX Hybridization oven rotator CGX Cot-1 Human DNA CGX Oligo acgh Hybridization Kit CGX Oligo acgh Wash Kit CGX DNA Labeling Kit Purification Columns CGX Stabilization and Drying Solution CGX DNA Labeling Kit CMG-2025 Prepito Circulating NA1k Kit, 6 samples CMG-1083 chemagic RNA Blood Kit special, samples CMG-1084 chemagic RNA Blood Kit special, 24 samples CMG-1093 chemagic RNA Saliva Kit special, 1 ml, samples CMG-1031 chemagic mrna/gdna Kit special, 2 ml blood or bone marrow, samples Product Number IVD Product Name Prenatal BoBs Luminex 200 w/ xponent BoBsoft 1.1 Analysis Software BoBsoft 2.0 Analysis Software 19

20 PerkinElmer, Inc. PerkinElmer, Inc. 940 Winter Street Wallac Oy Waltham, MA USA PO Box 10 Phone: (800) or Turku, Finland (+1) Phone: (+ 358) Fax: (+ 358) Signature Genomics/ PerkinElmer 2820 N. Astor Street Spokane, WA USA Phone: (+1) PerkinElmer chemagen Technologie GmbH Arnold-Sommerfel-Ring Baesweiler, Germany Phone: (+49) ISO ISO 9001 CMDCAS ISO OHSAS For a complete listing of our global offices, visit 20 PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All other trademarks depicted are the property of their respective holders or owners. PerkinElmer reserves the right to change this document at any time and disclaims liability for editorial, pictorial or typographical errors , December 20