Epigenetic Research: MethylHunter

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1 Epigenetic Research: MethylHunter MBD1-Based Methylated DNA Enrichment Kit High enrichment efficiency Consistent enrichment throughout genome loci Quick and easy protocol MBD1 belongs to the family of MBD (methyl-cpg binding domain) containing proteins and binds to methylated CpG DNA through its N-terminal MBD. The MethylHunter DNA Enrichment Kit can enrich DNA fragments containing methylated CpG DNA by using recombinant His-tagged MBD1. The concentrated DNA fragments can be used for following microarray, Real-Time PCR, as well as for sequencing analysis. Workflow Enriched methylated DNA fragments can be readily used for following microarray, Real-Time PCR, and Next Generation Sequencing analysis. MethylHunter MBD1-Based Methylated DNA Enrichment Kit Enrichment Performance The MethylHunter kit enriched the target methylated DNA loci by times. Order No. Description Amount 5270 MethylHunter MBD1-based Methylated DNA Enrichment Kit 25 Tests Phone: +49 (0) Fax: +49 (0)

2 Performa DTR: Dye Terminator Removal Systems Sequencing Purification & Cleanup Performa DTR utilizes a fully hydrated gel filtration matrix that provides for a convenient and simple method of dye terminator removal for sequencing cleanup. Performa DTR ensures removal of BigDye Terminator v3.1 or other dye terminators, along with dntps, salts, and other low molecular weight materials from sequencing reactions. DNA primers and fragments up to 20 bases, buffers, nucleotides labeled with biotin, isotopes, and other assorted markers are also eliminated. To minimize the potential for interference with sequencing applications no preservatives, salts, or buffers are used in the preparation of Performa DTR. Long read lengths More readable sequences Quick 2-spin protocol Ready-to-use Effective dye terminator removal of various chemistries (Selection) Performa DTR Gel Filtration Cartridges 108 Cartridges Performa DTR V3 96-Well Short Plate Kit 10 Plates MagSi-DNA Saliva Kit Fast and Cost-Effective Extraction of Genomic DNA from Saliva Sample collection is noninvasive, risk free, and painless Fast and easy processing using magnetic beads Suitable for automated liquid handlers Compatible with fresh saliva samples as well as sample collection and preservation kits Performa DTR V3 96-Well Short Plates are gel filtration plates that consist of 440 μl volume columns in a standardized array. Performa DTR Gel Filtration Cartridges are 0.8 ml spin columns packed with a gel matrix. Benefits More affordable than blood testing Protocol easily adjustable for different sample volumes Yields high quality genomic DNA Excellent purity, A 260/280 = Saliva is rapidly becoming an attractive alternative source of DNA for genetic testing. Unlike blood or tissues, saliva can be collected noninvasively by individuals with little training, and without the need for expensive equipment. Saliva preserved in collection kits is stable for years at room temperature, facilitating easy transportation and storage. The MagSi-DNA saliva kit is a magnetic bead-based kit for extraction of DNA from human saliva or buccal swabs. The kit includes magnetic beads, buffers, and Proteinase K, and is easy to automate for high throughput processing. DNA is suitable for use in downstream applications such as PCR, genetic typing, SNP, and mutation analysis as well as forensic analysis. MD60002 MagSi-gDNA Saliva Kit Preps MD61002 MagSi-gDNA Saliva Kit 10x96 10 x 96 Preps Phone: +49 (0) Fax: +49 (0) info@mobitec.com

3 Minute Total Protein Extraction Kit for Adipose Tissues The Role of Dysfunctional Adipose Tissue Adipose tissue, especially white adipose tissue, has been recognized as an important endocrine and inflammation organ. Isolation and analysis of proteins from adipose tissues are crucial for understanding many physiological/ pathological conditions, including the increasing risk of various types of cancer. We present a novel technology to successfully isolate proteins from adipose tissues. Very high concentration (2-3 mg/ml) of extracted proteins Porous filter with unique surface property and predefined pore size and thickness Detergent-free extraction buffer Rapid and effective separation of water-oil emulsion derived from adipose tissue homogenate Total proteins isolated are unbiased representation of cellular proteins in the tissue Images of Water-Oil Emulsion of Porcine WAT: Before (left) and after (right) passing through the filter. Hydrazide Resins Amino Acid-Preloaded Hydrazide Resins for Generation of Peptide Thioester Preloaded with 19 L- & 19 D-isomer amino acids For synthesis of peptide hydrazide using SPPS For synthesis of long or difficult peptides using peptide fragment coupling AT-022 Minute Total Protein Extraction Kit for Adipose Tissues / Cultured Adipocytes 20 Tests AF-023 Minute Adipose Tissue Fractionation Kit 20 Tests Native Chemical Ligation (NCL) has been one of the most used bioconjugation methodologies for synthesis of macromolecules. NCL requires a peptide thioester and another peptide moiety containing a free cysteine at the N-terminus. However, peptide thioester synthesis is technically challenging due to its instability. The use of a peptide hydrazide is more recommended. As a worldwide unique provider of amino acid-preloaded hydrazide resins, AnaSpec offers a series of 38 ready-touse hydrazide resins carrying one of the 19 natural L- or D-isomer amino acids. These resins are perfectly suited for the production of activated peptide thioester via Solid Phase Peptide Synthesis (SPPS). Generation of Peptide Thioester on AA Hydrazide Resins A peptide hydrazide is generated after SPPS and cleavage and converted into a peptide thioester. Bioconjugation via NCL Generated peptide thioester can be conjugated by NCL between an unprotected C-terminal thioester peptide and a Cys/Ala-containing peptide/protein. Peptide thioesters are commonly used for bioconjugation to protein, dye, drug like DOTA, or printing to glass surface. Phone: +49 (0) Fax: +49 (0) info@mobitec.com

4 Fluorescent Dyes Brochure Available For Labeling and Detection Technical notes on fluorescence Fluorescence instruments Selection of fluorescence reagents Reactive fluorescent dyes and their applications Reactive dyes HiLyte Fluor dyes Classic reactive dyes QXL quenchers Anatag protein labeling kits Calcium and physiological indicators Nucleic acid dyes Biotins and streptavidins Contact us to get your printed version or download the PDF at Cryogenic Vials Tubes for Freezing Preservation Suitable for mouse sperm and embryo vitrification Outer cap type and inner cap type available Caps are color coded Excellent airtightness Easy pipet operation Cryogenic Vials MS-7550 Series are provided in Grey, Green, and Pink colors, making sample classification easy. Sumitomo s Cryogenic Vials are suitable for the safe storage of valuable samples such as cells, serum, and antibodies. Compact Rack The Compact Rack for Cryogenic Vials can withstand storage in a deep freezer (-80 C). Vitrification In cryopreservation vitrification is a common method used to preserve egg cells and embryos. Of utmost importance is that the oocyte or embryo is stored in a vessel that allows appropriate temperature modulation. The quality of cryotubes may affect the survival rates of embryos after freezing-thawing. Therefore, it is essential to examine the inside surface of the cryotubes for its smoothness before vitrifying embryos. Sumitomo Bakelite Co., Ltd., Japan, is a pioneer in plastics. Their Cryogenic Vials are proven suitable for the purpose of cryopreservation of mouse embryos (Mochida et al. (2011); J. Vis. Exp. (57)). Lock Stand Using the Lock Stand, the vials enable one-handed screwing of the cryogenic vial cap. (Selection) Order No. Description Amount MS-4603WZ 2 ml Cryogenic Vial, Cap Color: White 500 Vials MS-7550LZ Rack for Cryogenic Vial with Lid (Grey) 10 Racks MS-78000Z Lock Stand for Cryogenic Vial 5 Pieces Phone: +49 (0) Fax: +49 (0) info@mobitec.com

5 ELISA Kits for Educational Purposes Kits for Laboratory Method Lessons at High Schools and Universities Demonstration of ELISA principle Testing at laboratory temperature No need for any special equipment No hazardous substances or infectious material Total test time 35 minutes Detailed manual (for teacher) and leaflet with the test procedure (for students) ELISA-VIDITEST EDUCO Diagnostic Aim of the test: Antibody detection in unknown samples. ELISA-VIDITEST EDUCO Vitamin Aim of the test: To determine the approximate content of vitamin H in food samples. ODZ-191 EDUCO Diagnostic (Made-to-Order Production*) 96 Tests ODZ-351 EDUCO Vitamin (Made-to-Order Production*) 96 Tests *Please take into consideration corresponding delivery times. Carbohydrates from Dextra Carbohydrate Analysis, Synthesis, and GMP Manufacturing Monosaccharides Nucleosides Phosphorylated sugars Umbelliferyls Imino sugars Oligosaccharides Heparin & Chondroitin derived oligosaccharides Maltooligosaccharides Blood group products Glycans Neoglycoproteins Neoglycolipids Polysaccharides Pharmaceutical intermediates and diagnostics Dextra has gained a world-wide reputation for the supply of rare sugars, oligosaccharides, and chiral molecules for medical research, diagnostics, and pharmaceutical applications. With over 1000 complex sugars available, they are the most comprehensive source of products for exploiting the complexity and diversity of glycobiology. Phone: +49 (0) Fax: +49 (0) info@mobitec.com

6 Companies distributed by MoBiTec GmbH Clare Chemical Research MagnaMedics ProFoldin For the most up-to-date listing of our supplier companies please visit our website MoBiTec GmbH, Lotzestrasse 22a, Goettingen Phone: +49 (0) Fax: +49 (0)