The Thermo Scientific KingFisher Family

Transcription

1 The Thermo Scientific KingFisher Family Accelerate your genomics and proteomics throughput with the Thermo Scientific KingFisher purification systems Part of Thermo Fisher Scientific

2 The Thermo Scientific KingFisher family high speed purification of proteins, nucleic acids and cells Our KingFisher range of exciting high speed DNA, RNA and protein purification systems are designed to resolve all your purification problems, now and in the future. Based on revolutionary patented magnetic separation technology, KingFisher systems enable you to process any sample - proteins, nucleic acids and cells from virtually any source including blood, cell cultures, tissue lysates, soil and faeces with guaranteed sample integrity. Truly amazing operational speeds allow up to 96 samples to be processed in under 15 minutes. The Thermo Scientific KingFisher purification system offers you: Fully automated high speed purification High throughput 96 sample processing Purification of any sample from any source Pre-defined and custom protocols Easy operation via instrument or external PC The KingFisher family comprises of three systems, KingFisher, KingFisher ml and - covering working volumes from µl. Each system consists of an instrument, specially designed plastics and easy-touse software. With optimized protocols you can be up and running in a matter of minutes. KingFisher µl in microstrips, up to 24 samples per run KingFisher ml µl in tube strips, up to 15 samples per run µl in 96 and 24 deep well, 96 KF, and 96 PCR plates, up to 96 samples per run Solve those purification problems Speed and sample purity continue to be a major concern to genomics and proteomics researchers. The sheer volume of work involved in genome and protein mapping means processing speed is essential. Likewise, unsatisfactory results on even the most sophisticated analysers are increasingly linked to poor initial sample quality. However the variety of samples being used, from rare cell types and mrna transcripts to increasingly large and delicate protein complexes, presents a real challenge. On all counts, KingFisher automated purification systems ensure impurities are removed. The KingFisher range of applications includes: Genomics & proteomics Target identification Forensics Biomarker discovery Quality control Veterinary assays

3 Work at unparalleled speed The processing speed of KingFisher systems is unparalleled and stems from their advanced magnetic particle processing technology which uses magnetic rods to move sample particles through the various purification phases binding, mixing, washing and incubation - eliminating liquid handling and increasing automation results in a reliable and robust technique. The overall efficiency brings speed for true market-leading throughput. The top of the range can purify 96 samples in just minutes. Equally important, sample cross contamination and reagent cross-over is effectively eliminated by the automated purification process. Adapt to your own routine KingFisher enables you to increase your workflow flexibility. Pre-defined application protocols are available for many existing magnetic particle-based methods. Additionally, it is easy to customise existing protocols or create entirely new ones using the intuitive Thermo Scientific BindIt TM software. Protocols can then be run either directly via the KingFisher instrument or remotely via a PC. See instant benefits in productivity Installing a KingFisher system will make an instant impact on your work. You will be able to purify samples at speeds you never believed possible, with minimal hands-on requirement as the automation takes over. KingFisher will leave you free to concentrate on more involved downstream analysis. The Thermo Scientific KingFisher family Thermo Scientific Using either a 96 or 24 plate format, provides the fastest and easiest way to purify genomic DNA or RNA and proteins from a variety of sample material. Supplied with predefined protocols, also allows you to create your custom made protocols for special applications. Samples/run 96 or 24 Working volume in µl 24-format 96-format Thermo Scientific KingFisher KingFisher gives you the ability to purify small-scale samples. All purification and processing steps are carried out in microplates with simple push button operation. Samples/run 24 Working volume in µl Thermo Scientific KingFisher ml KingFisher ml is your choice for high volumes. It allows all purification and processing steps to be carried out in a single strip of five tubes. Being able to go as low as 50 µl to release target molecules, enables cells and proteins from larger starting volumes to be isolated and concentrated simultaneously. Samples/run 15 Working volume in µl

4 Thermo Scientific KingFisher Plastics KingFisher instruments utilise disposable plastics made of polypropylene and they are specially designed for the processing and isolation of target molecules. The plastics are ideal for magnetic particle processing due to their low binding affinity for biomolecules. KingFisher ml processes samples in tube strips whilst KingFisher and utilise specially designed microplates. The magnets are protected by disposable tip combs. The design of these specialist plastics enables excellent recovery of magnetic particles. 24 deep well magnet head and tip comb 24 deep well plate Thermo Scientific Microtiter deep well plates, King- Fisher 96 KF plates and 24 deep well plates are all specially designed for and enable efficient processing and mixing of magnetic particles. Both the yield and quality of the isolated DNA/ RNA is significantly improved with special KingFisher plates. In addition fully skirted rigid PCR plates can be used with. High resistance to organic solvents High temperature resistance (-196 to +121 ºC) 96 deep well magnet head and tip comb Microtiter deep well 96 plate Sealable with adhesive films 96 deep well plate sealable with cap mat Thermo Scientific BindIt Software - the perfect complement to a peerless system BindIt Software is designed to allow you to develop custom made protocols or modify existing protocols for your own applications, allowing increased flexibility. Additionally, BindIt Software enables you to set user control access levels, advanced protocol management, sample information and clear reporting. Pre-defined and validated purification protocols are available for different applications, offering the possibility to choose the best possible method for a sample process. The BindIt Software also enables the to interface with liquid handling, robotics and plate stacking instruments, providing a fully automated solution and the highest throughput. 96 KF plate PCR magnet head and tip comb 96 KF magnet head and tip comb 96 KF plate 96 PCR plate 96 KF plate magnetic heads with tip combs and suitable microplates.

5 Thermo Scientific KingFisher applications KingFisher provides a quick and easy solution to achieve high quality and reproducible results in the purification of nucleic acids, proteins and cells with minimal hands-on time. It is an open and flexible system with a wide working volume range, allowing the user to choose any available magnetic particle based purification kit suitable for the application. With many applications available on the KingFisher website ( it is easy to create a new protocol for a specific application with the BindIt Software. Flexible solutions for nucleic acid purification Magnetic particle based kits offer excellent performance for the purification of DNA and RNA with KingFisher, KingFisher ml or. The range of starting material for nucleic acid research is broad from 1ml of blood for routine testing to very small samples e.g. from forensic surface and contact swabs, hair and cigarette butts. KingFisher enables you to isolate DNA or RNA in sufficient quantities from all kinds of material sources such as serum, blood, bacteria, cell culture, tissue, soil or faeces. Plant kits can be used to purify DNA from several plant species and sources e.g. leafs and seeds. Using magnetic bead based separation technology ensures the resulting DNA and RNA is free of protein, nucleases and other contaminants ensuring your purified nucleic acid samples are ready to use in downstream applications. With KingFisher instruments it is possible to save time and minimise manual handling steps including pipetting, centrifugation and mixing. Automation of the purification process and elimination of any liquid handling steps result in reliable and robust operation. High speed protein purification KingFisher can be used to purify all kinds of proteins from recombinant to therapeutic antibodies. The magnetic separation technology solves the problem of purifying recombinant proteins with highly complex structures and varying physiochemical properties. The KingFisher technology and range of chromatography beads enable routine purification of antibodies/ antigens, pharmaceuticals and vaccines using any kind of host cell mammalian, insect, yeast, bacteria or transgenic animals/plants. KingFisher can also significantly improve the phage display process by fully automating the cycles of the biopanning phase. The BindIt Software allows you to develop your own robust protocols for other protein applications like glycoprotein purification, protein arrays, protein interaction or protein phosphorylation studies. KingFisher Flex 24 Spin Column Hands on Hands free time (min) 0:00 0:30 1:00 1:30 2:00 2:30 Figure 1. The time used for purification of DNA from 1ml of blood. The comparison of isolating 24 samples between and a column based isolation method. Lambda / Hind III KIT 1 KIT 2 KIT 3 3B 3C 3D 4B 4C 4D 3B 3C 3D 4B 4C 4D 3B 3C 3D 4B 4C 4D Lambda / Hind III Figure 2. The quality and quantity of genomic DNA isolated from blood with is excellent. KingFisher purification process BIND sample with beads WASH to get rid of impurities ELUTE purified product Figure 3. Rapid and robust sample preparation.

6 Technical specifications Thermo Scientific Thermo Scientific KingFisher ml Thermo Scientific KingFisher Processing volume 96: µl µl µl 24: µl Capacity samples/run 96 or Collection efficiency 95% 95% 99% of the particles Magnetic rods 96 or Plate types 24 deep well plate ( µl), 96 deep well plate ( ), KingFisher 96 plate Special tube strip, 1 x 5 tubes, µl tube Special microstrips, 1 x 8 wells, 8 x 12 grid format, and µl well volumes ( µl), PCR plate ( µl) Tip combs Special design, 96 or 24 format Special design, 1 x 5 format Special design, 1 x 12 format Heating temperature Heating block temperature from room Ambient, no heating Ambient, no heating temperature to +115 C Magnetic particle ca. > 1 µm ca. > 1 µm ca. > 1 µm size Keyboard / Display START/STOP/PAUSE/OK, four arrow keys, START/STOP/two cursor keys/lcd START/STOP/two cursor keys/lcd two keys to rotate the turntable, color LCD Internal Software Space for ca. 100 internal protocols Space for ca. 8 internal protocols Space for ca. 8 internal protocols BindIt Software yes yes yes compatibility Computer interface USB or serial RS-232C port Serial RS-232C port Serial RS-232C port Robot compatibility Yes No No Size WXDXH mm ca. 680 x 600 x X 290 X X 290 X 310 Weight kg BindIt Software specifications: Supported operating Microsoft Windows XP Professional with SP2 or later and Vista. Internet Explorer 6.0 or later must be installed. system Used to create, modify and run the protocols with KingFisher instruments Ordering Information KingFisher, Magnetic Particle Processor, V, 50/60 Hz (for microplates) KingFisher ml, Magnetic Particle Processor, V, 50/60 Hz , Magnetic Particle Processor, with 96 PCR head, V, 50/60 Hz , Magnetic Particle Processor, with KF head, V, 50/60 Hz , Magnetic Particle Processor, with 96 deep well head, V, 50/60 Hz , Magnetic Particle Processor, with 24 deep well head, V, 50/60 Hz Software: BindIt Software, CD and user manual Accessories and spare parts: PCR head and heating block KF head and heating block deep well head and heating block deep well head and heating block KingFisher ml tube tray KingFisher ml microtube tray PCR head KF head deep well head deep well head KF Flex 96 PCR heating block KF Flex 96 KF heating block KF Flex 96 deep well heating block KF Flex 24 deep well heating block Consumables for KingFisher: KingFisher tip comb, 50 pcs KingFisher plate 100 µl, 50 pcs KingFisher plate 200 µl, 50 pcs KingFisher plastics 100 µl 8-pack, 8 plates (100 µl) + 8 tip combs / box KingFisher plastics 200 µl 8-pack, 8 plates (200 µl) + 8 tip combs / box Consumables for KingFisher ml: KingFisher ml tip comb, 800 pcs KingFisher ml tube, 900 pcs (20x45 pcs) KingFisher ml Combi 60 (tubes and tip combs for 60 samples) KingFisher ml Combi 240 (tubes and tip combs for 240 samples) Consumables for : tip comb for PCR magnets, 80 pcs tip comb for KF magnets, 100 pcs tip comb for deep well magnets 100 pcs deep well tip comb and plate, 50 pcs of each KF plate (200µl) 48 pcs Microtiter deep well 96 plate, V-bottom, polypropylene 50 pcs Microtiter deep well 96 plate, V-bottom, sterile polypropylene 50 pcs deep well plate, 50 pcs deep well plate sterile, 50 pcs North America: USA / Canada Europe: Austria Belgium France Germany national toll free Germany international Italy Netherlands Nordic countries Russia/CIS +7 (812) Spain/Portugal Switzerland UK/Ireland Asia: China or India Japan Other Asian countries Countries not listed: or kingfisher Thermo Fisher Scientific/Suomen Painotuote/BRO-MIB-KINGFISHER Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.