1.Gene Synthesis. 2.Peptide & Phospho-P. Assembly PCR. Design & Synthesis. Advantages. Specifications. Advantages

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1 1.Gene Synthesis Assembly PCR Looking for a cdna for your research but could not fish out the gene through traditional cloning methods or a supplier? Abnova provides a gene synthesis service via assembly PCR to construct the desired cdna from a specified and optimized codon sequence.. Abnova performs all over-lapping primer design, oligo synthesis, assembly PCR, gene sequencing and cloning into a stable vector for gene target amplification. The derived cdna is made available to the customer or can then be utilized for custom protein expression, polyclonal and monoclonal antibody production. Advantages Create cdnas of Interest Automated Nucleotide Synthesis Mutation-Free Sequence Optimized Codon Usage Fast Turn Around & Stable Quality 2.Peptide & Phospho-P Design & Synthesis Abnova offers a complete advisory service on peptide/phospho-peptide design and high quality custom synthesis for all tasks in research and antibody production. Let us know your desired peptide sequence, length, purity, and quantity, and we will take care of the rest. Abnova also provides labeling, modification and conjugation services to match your specific needs! Specifications Length : 5-50 amino acids Purity : Crude, >70%, >85%, & >90% Quantity : 1mg 500mg Labeling & Modification: Biotinylation, Phosphorylation Conjugation : KLH, BSA, Thyroglobulin Advantages Dependable Quality Competitive Pricing Fast Turn-Around Time Purity to Match Your Applications

2 Applications Polyclonal and Monoclonal Antibody Production Antibody Epitope Mapping Screening Assays for Biological Activity Enzyme Profiling Epitope Mapping 3.Protien Expression Custom In itro Wheat Germ Protein Expression Do you have a protein you would like us to express for you? We will use our robust in vitro protein expression technology to service your needs. Customers have the option to perform a test-scale expression to assess the yield and solubility of their protein of interest, and then scale up to the larger quantity required by the experiment. See Technology General Working Progress of Custom Protein Expression Evaluation of Standard Protein Expression Service Pre-Expression Discussion: Custom Specification, Flowchart, Cost Purchase Order Expression Specification Approval Service Agreement Protein Expression Report Protein Delivery Related FAQ Service 1 : Test-Scale Protein Expression Protein Expression Specification Template ( PDF, Excel File) In itro Wheat Germ Expression Flowchart Duration : 1.5 month Service Agreement Total Price : Quote for price Service 2 : Scaled-Up Protein Production Duration: 4~6 weeks (depending on the desired quantity) Total Price (depending on test-scale result)

3 4.Ab Production Mouse Custom Monoclonal Antibody Production Abnova understands the importance of monoclonal antibody for your research. We offer monoclonal antibody (Mab) development in mice, with guaranteed success based on standard endpoints or customer's specific applications. Our high rate of success, short turn-around time and affordable cost is due to our hybridoma technology, state-of- the-art mouse facility, extensive experience in antibody production for over 4,000 gene targets, and flexibility to suit our customer's needs. General Working Progress of Monoclonal Antibody Development Evaluation of Standard Antibody Production Service Pre-Production Discussion: Custom Specification, Flowchart, Cost Purchase Order Production Specification Approval Service Agreement and Production Specification Template (PDF, Excel File) Monoclonal Antibody Development and Progress Report Monoclonal Hybridoma and Supernatant Delivery Related FAQ Download Custom Mab Service (PDF file) Custom Mab Services Standard Package Premium Package Payment 1 (USD/EUR) Non-refundable startup fee 1,800 Quote Phase I Antigen Preparation Customer-supplied antigen (protein) * Please quote if the customer requires antigen prepared by Abnova** Customer-supplied antigen (peptide) * Please quote if the customer require antigens prepared by Abnova** Immunization Number of mice immunized will be specified by the customer Immunization of mice and hybridoma fusion Phase II Fusion & Screening ELISA screen up to 1000 hybridoma clones Stabilization of up 10 positive clones Any mouse that shows a customer's required titer will be used to create ELISA positive monoclonal antibodies Final Deliverables ELISA data and up to 10 clones of ELISA-positive hybridoma clones*** and culture supernatants (3ml/clone)

4 The ELISA-positive monoclones will be delivered to customer in the format of 200 μg purified Mabs. Customer will test the supernatants on specific applications to determine the number of positive monoclones Payment 2 (USD/EUR) Non-refundable 2,800 Quote (per mouse hybridoma project) Lead time (Month) 3~3.5 Phase III Hybridoma Delivery Payment 3 Customer will request hybridomas deemed suitable for customer's needs and pay Abnova for each hybridoma*** (USD/EUR) Payment 3 is only paid upon hybridoma delivery Quote (per hybridoma) Total Price (USD/EUR) 4,600 Starting at 10,000 *Antigen Requirement (provided by customer): Protein Immunization: 0.3~0.6mg Peptide Immunization: Conjugated-peptide (180ug) for immunization and Free-Peptide (160ug) for screening step **Immunogen Provided by Abnova: Purified recombinant protein: additional 2,300 USD/EUR service fee will be charged by Abnova. Setup fee 800 USD/EUR, or Peptide: additional 1,000 USD/EUR service fee will be charged by Abnova. Setup fee 1,000 USD/EUR, or Phosphopeptide: additional 1,500 USD/EUR service fee will be charged by Abnova. Setup fee 1,500 USD/EUR, or Abnova may obtain from a source subject to customer's approval, and invoice the cost to the customer. Lead time: 1~1.5 month ***Cell Line Storage (3 vials/cell line/year) - 50 USD/EUR 5.IgG Amplification Custom Ascites Production Abnova will match your choice of cell lines to our tailored protocols, a preferred strain of mice, and production scale. Customers will provide cell line(s) of interest to Abnova for the production of mouse ascites. The custom ascites production service is based on number of mice used to generate the quantity of ascites needed by our customer. Average ascites yield for Balb/c is 3ml, and for SCID is 3ml. Actual yield will vary for each cell line.

5 Balb/c 20 mice / cell line 40 mice / cell line Related FAQ 100 mice / cell line 1-25 cell lines $1,120 / cell line $2,240 / cell line $5,320 / cell line cell lines $1,064 / cell line $2,128 / cell line $5,054 / cell line > 99 cell lines $1,008 / cell line $2,016 / cell line $4,788 / cell line Additional $50 will be applied for mycoplasma test. We offer discount for large order, please contact our sales and marketing department for further detail. SCID/Nude 20 mice / cell line 40 mice / cell line 100 mice / cell line 1-25 cell lines $1,800 / cell line $3,600 / cell line $8,550 / cell line cell lines $1,710 / cell line $3,420 / cell line $8,122 / cell line > 99 cell lines $1,620 / cell line $3,240 / cell line $7,694 / cell line Additional $50 will be applied for mycoplasma test. We offer discount for large order, please contact our sales and marketing department for further detail. Extra Service Service Item Price Antibody Purification (100 ml of ascites for monoclonal antibody purification at 70% recovery rate) $1,200 Isotype Test $100 Additional $50 will be applied for mycoplasma test. We offer discount for large order, please contact our sales and marketing department for further detail.

6 6. Conjugation Antibody / Protein Conjugation Abnova offers high quality, efficient and cost-saving conjugation service between antibody/protein and fluorescent dyes, phycobiliproteins (RPE, APC, cl-apc), FITC, streptavidin, biotin and horseradish peroxidase (HRP). Since every antibody/protein has its own unique characteristics, conjugation needs to be optimized. 0.2mg of total antibody/protein of interest will be tested and HPLC analyzed prior to mass labeling. The tested sample will be mixed with mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the antibody/protein sample. General Working Progress of Antibody Conjugation Evaluation and Discussion of Conjugation Requirement and Service Completion of the Custom Conjugation Specification (PDF, Excel File) Confirmation of Receipt of Antibody/Protein Sample Conjugation Buffer Exchange Antibody/Protein Concentration and olume Analysis Test-Scale Conjugation Optimization Mass Antibody/Protein Labeling Chromatographic Gel Purification Spectrophotometer Measurement COA, Packing, and Shipment Table of Labeling Molecules and Excitation/Emission Wavelengths Labeling Molecule Excitation Max (nm) Emission Max (nm) RPE 495, APC cl-apc (PEAK) 495, FITC Special Notes: 1. The antibody/protein of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of antibody/protein from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer. 2. Minimum quanity of antibody/protein conjugation is 0.1mg. Minimum concentration is 0.2mg/ml.

7 7.Immunohistochemistry Immunohistochemistry Service Abnova provides an expanding library of more than 8,500+ immunohistochemistry (IHC) antibodies on its online catalogue. Our capability is supported by a vast collection of normal and diseased tissue samples for antibody characterization. In addition, our inhouse IHC team has created panels of tissue microarray for high throughput of antibody validation, biomarker discovery, and companion diagnostic development. We are eager to learn of our customers specific requirement and to facilitate their research and product development! Abnova's Resource Normal and Diseased Clinical Samples Pre-made Tissue and Microarray Slides Expanding Monoclonal Antibody Catalogue Antibody Production Infrastructure Rich Experience and Proven Expertise Customer's Resource Provide Own Clinical Samples Provide Pre-made Tissue Slides Antibodies (with or without Characterization) Specific Tissue and Antibody Protocols Previous Experimental Results General Workflow of IHC Service Customer completes a IHC custom service form (PDF, Excel File) and send to Abnova. Abnova prepares slides from formalin-fixed and paraffin-embedded tissues. Abnova performs IHC staining either on slides without pretreatment or with pretreatment, or both. Abnova performs dilutions of antibody according customer's instruction or performs a serial dilution of 40, 20, 10, 5, 2.5, 1.25, ug/ml. Abnova offers the following slide pretreatments. Customer may choose one or more protocols for the pretreatment : 1. Pretreatment in 1X citrate buffer (ph 6.0), microwave at 750W for 20 minutes, and subsequently cool the samples. 2. Pretreatment in 1X Tris/EDTA buffer (ph 9.0), microwave at 750W for 20 minutes, and subsequently cool the samples. 3. Pretreatment in HCl (2N) (ph 0.6~0.9) at room temperature for 10~20 minutes. 4. Pretreatment in 0.1% trypsin and shake for 25 minutes at 37C.

8 Strict Confidentiality Abnova will sign a confidential agreement with the customer and will not to release any experimental results to the third party without customer's permission. Table of IHC service contents and service charge Service Charge (USD) Service Options set up Additional charge Antibody Slide Note Customer's antibodies & slides Customer's slides & Abnova's antibodies Customer's antibodies & Abnova's Slides 150 USD 1 ~ 10 slide, 10 USD per slide > 10th slide, 5 USD per slide USD per Ab USD per slide If customer provide tissue block instead of tissue slide, here will be an extra charge of 50 USD / blockfor processing fee Abnova's antibodies & slides 100 USD per Ab 10 USD per slide

9 8. Assay Development Antibody Pair for Sandwich ELISA Abnova provides a total solution for antibody pair development for quantitative measurement of your protein of interest. Leveraging our mouse monoclonal antibody manufacturing capability, and our new MaxPab technology for production of rabbit polyclonal antibody from full-length protein, Abnova allows you to focus on more important parts of your research instead of the arduous task of searching and testing for the matching antibody components. You may use our antibody pair in conventional sandwich ELISA or system applications. General Working Progress cdna Sourcing and Cloning Recombinant Protein Expression and Purification Mouse and Rabbit Immunization Screening Matched Mouse Mab and Rabbit Pab Standard Curve Construction using Recombinant protein alidation on Mammalian Transfected Lysates for Native Protein Recognition Delivery of Mouse Hybridoma and Rabbit Pab Serum Advantages High Specificity High Signal to Background Ratio Multiple Epitopes Target Detection Amenable to S-ELISA and System Applications Download Antibody Pair Service (PDF file)

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