Report on the BioImagine Nencki Microscopy Workshop. organizers: Grzegorz Wilczyński, Jakub Wlodarczyk, Tytus Bernaś

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1 Report on the BioImagine Nencki Microscopy Workshop organizers: Grzegorz Wilczyński, Jakub Wlodarczyk, Tytus Bernaś The BioImagine event Nencki Microscopy Workshop took place at the Nencki Institute on May 28 th -31 st. The workshop comprised both, lectures and training in the advanced microscopy systems and techniques available at the Nencki Institute. The main aim of the event was to present a frontier microscopy research and methodologies to the broad audience. The lectures were given by international microscopy experts who covered a broad array of techniques: Yannick Schwab from EMBL, Heidelberg (correlative microscopy), Jonas Ries from EMBL, Heidelberg (superresolution microscopy), Edward Lemke from EMBL, Heidelberg (novel biosensors for cell metabolism), Evgeni Ponimaskin from Medical University of Hannover (imaging of plasma membrane receptors), Andre Zeug from Medical University of Hannover (FRET in cell biology), Yuri Prokazov from Leibniz Institute, Magdeburg (FLIM detectors), Andrew Woehler from Max Planck Institute, Goettingen (multiplex FRET biosensors), and Ireneusz Grulkowski from Nicolaus Copernicus University, Toruń (mesoscopic imaging by optical coherence tomography) (see the program below). In addition to full length lectures, short talks were given by speakers

2 selected from participants, based on the quality of research presented in abstracts: Matthia Winter-Karreman, Adriana Magalska, Alexander Wirth, Michał Stawarski, Antonina Khoruzhenko, Kamil Parobczak, Agnieszka Walczak. The practicals were organized in the microscopy core facility of the Nencki Institute, and given by experienced microscopists. The following systems and techniques were presented: 1) Live-cell confocal imaging of primary neuronal cultures, by Joanna Dzwonek, Anna Skupień, and Marta Pyskaty, on Zeiss 780 LSM, 2) Fluorescence correlation spectroscopy, by Jędrzej Szymański, on Leica TCS SP8, 3) Automatic 3D reconstructions in electron microscopy, by Wojciech Brutkowski and Paweł Trzaskoma, on Zeiss Sigma FESEM equipped in 3View system of Gatan, 4) Clarity technique of imaging of transparent brain, by Michał Stawarski, using Zeiss-Yokogawa spinning disk microscope, 5) Highpressure freezing and freeze-substitution processing of tissue for electron microscopic immunogold studies, by Andrzej Szczepankiewicz and Kamil Parobczak, using Leica EMPACT 2 and AFS machines, 6) Fluorescence life-time imaging, by Jarosław Korczyński, on Leica TCS SP8.

3 The number of partcipants attending the workshop was 103 (see the list of names at the end of this document). The participants came from various academic centers of Poland, from Ukraine and from Germany.

4 The poster:

5 Pictures: Ireneusz Grulkowski Multidimensional interferometric imaging and metrology in biomedicine from microscopy to mesoscopy Yannick Schwab Correlative Light and Electron Microscopy: taking snapshots of the living at the ultrastructural level

6 Matthia Winter Karreman Lights Will Guide You: Tracking Invasive Tumor Cells with Correlative Intravital Fluorescence Microscopy and Electron Microscopy Evgeni Ponimaskin Regulation of receptor functions by heterodimerization of serotonin receptors

7 Jonas Ries Superresolution Microscopy of Protein Structures in Situ Adriana Magalska Loss of neuronal 3D chromatin organization causes transcriptional and behavioral deficits related to serotonergic dysfunction

8 Edward Lemke Decoding molecular plasticity from single molecules to large assemblies Andre Zeug How to visualize protein s function: Quantitative molecular microscopy approaches

9 Alexander Wirth Cellular functions of Cdc42 are dependent on lipid modifications Yury Prokazov From large field microscopy to single photon counting

10 Andrew Woehler Mulitsensor FRET microscopy: towards spatiotemporal correlation of intracellular signaling processes Antonina Khoruzhenko mtor association with nucleoli and intermediate filaments in human normal and breast cancer cells

11 Katarzyna Rojek Expression and function of Angiomotin family of proteins in the brain Kamil Parobczak Arc functional neighborhood in the nucleus

12 Agnieszka Walczak Spatial repositioning of the Bdnf gene upon seizures Błażej Ruszczycki Before practicals: computational methods in (neuro)biological image analysis

13 The audience Practicals: live cell imaging

14 Practicals: 3View Practicals: Clarity