Programme PhD / Master course Advanced Microscopy and Vital Imaging Time Item Location Welcome and brief overview of the course

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1 Programme PhD / Master course Advanced Microscopy and Vital Imaging 2014 Monday, June 2, Welcome and brief overview of the course Basics of Microscopy Practical course: Bright field microscopy, a hands-on course GROUP I UNS 40 C /Helma Kuijpers [] Maastricht University Medical Center Fluorescence microscopy GROUP II Fluorescence microscopy GROUP I Practical course: Bright field microscopy, a hands-on course GROUP II UNS 40 C /Helma Kuijpers [] Maastricht University Medical Center

2 Tuesday, June 3, Principles of confocal scanning laser microscopy Edwin Lamers Carl Zeiss Benelux Live cell imaging using GFP-technology in combination with CSLM Sample preparation GROUPS Sample preparation Demonstrations (according to schedule, 45 min per group) Confocal scanning laser microscopy / Helma Kuijpers Dept. Molecular Cell Biology, Maastricht University Medical Center Demonstrations (according to schedule, 45 min per group) GROUPS 5-8 G.5.223A Confocal scanning laser microscopy / Helma Kuijpers Dept. Molecular Cell Biology, Maastricht University Medical Center G.5.223A

3 Wednesday, June 4, Analysis of the neuromuscular junction quantitative microscopy Mario Losen Dept. of Basic Neurosciences, Maastricht University Medical Center Backgrounds and applications of STED Microscopy Patrick van Wieringen Leica Microsystems BV Coffee/tea break Two-photon laser microscopy for the three-dimensional study of tissues ImageJ GROUPS ImageJ Demonstrations (according to schedule, 45 min per group) GROUPS 1-7 Two-photon scanning laser microscopy 0f Pc ruimte Of pc ruimte H Timo Rademakers [Zhuojun Wu] Sanquin, Amsterdam

4 Thursday, June 5, Advances in multiphoton microscopy in vivo imaging in cardiovascular research Timo Rademakers Sanquin, Amsterdam High throughput and high content cellular analysis for systems biology Winnok De Vos Ghent University Coffee/tea break Advanced Electron Microscopy Peter Peters EM-Unit and Dept. Molecular Cell Biology, Maastricht University Medical Center Departure to FEI Nanoport Company, Eindhoven Peter Peters /Helma Kuijpers EM-Unit and Dept. Molecular Cell Biology, Maastricht University Medical Center Welcome and introduction biological application of electron microscopy (by bus) Wim Busing Sr Applications Specialist NanoBiology, FEI Company, Eindhoven Demo's on Quanta 3D FEG (SEM), Quanta 250 (SEM), and Tecnai 20 (TEM) Wim Busing Sr Applications Specialist Nano Biology,FEI Company, Eindhoven Departure back to Maastricht University Medical Center FEI, Eindhoven (by bus)

5 Maastricht Microscopy Meeting (M 3 ) Friday, June 6, 2014 Symposium Advanced Microscopy and Vital Imaging Greepzaal, level 4, azm Maastricht University Medical Center, P. Debyelaan 25, Maastricht Arrival & Coffee Welcome by Dept. of Molecular Cell Biology, Maastricht University Medical Center Speaker Speaker 2 xx Speaker 3 xx Lunch Speaker 4 Dept. ofxxxxxx Speaker 5 x Speaker Speaker Closure & Drinks Participation is free, but please note that registration is obligatory. You can register by sending an , including your name and department, to the secretary of Molecular Cell Biology, Maastricht University Medical Center. secr-mcb@maastrichtuniversity.nl Telephone

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