Discover Cell-specific Genomics Arcturus PixCell IIe Laser Capture Microdissection System and RNA Isolation / Amplification Kits

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1 Bucher Biotec AG Schützengraben Basel Phone May 02 Discover Cell-specific Genomics Arcturus PixCell IIe Laser Capture Microdissection System and RNA Isolation / Amplification Kits Microdissection has grown to become an important tool in life sciences research. With the advent of latest laser microdissection technology and the popularization of genomic and proteomic tools more groups are using microdissection techniques to study cells than ever before. The technique allows the researcher to Arcturus PixCell IIe New Laser Capture Microdissection System and RNA Isolation/Amplification Kits Cepheid SmartCycler with Real Time Detection Essen Instruments PIPELINE A better way to dispense sterile liquids New Fluorescence Reader Molecular Devices Model Gemini EM New IMAP Generic Kinase and Phosphatase Assays choose exactly what cells he wants to analyze with genomic and proteomic tools. This ability to be selective allows studying different populations of cells (normal and mataplastic) within close proximity to one another. Laser capture microdissection (LCM) has been invented at the National Institutes of Health, Bethesda, Md, in 1996 and li- censed by Arcturus who developed a microgenomic platform that integrates complete sets of tools to extract and analyze pure cell populations to reveal cell-specific molecular signatures, going from the biopsy sample to the microarray. The PixCell IIe LCM system enables the acquisition of homogeneous cell populations from all cell types for gene expression studies with high specificity and integrity. It allows for fast, nondestructive cell selection from tissue sections or cell smears. cont. page 2 A new Twist in DNA Purification and Solid Phase Extraction: The Hamilton AVS Pyrosequencing extends genotyping system capabilities Invitation to MipTec 2002 Rapid DNA / RNA Amplification with Real Time Detection The Cepheid Smart Cycler The Smart Cycler System invented at the Lawrence Livermore National Laboratories and licensed to Cepheid is a highly versatile and efficient thermal cycler with real time optical detection, specifically tailored to the rapidly evolving needs of today's molecular biology laboratory. The core of the Smart Cycler System is the I-Core (Intelligent Cooling/Heating Optical Reaction) module, incorporating state-ofthe-art microfluidic and microelectronic design. Each Smart Cycler processing block contains sixteen independently programmable I-CORE modules, each of which performs four-color, real time fluorometric detection. Samples are amplified and measured in the Smart Cycler's proprietary sealable reaction tubes, which are designed to optimize rapid thermal transfer and optical sensitivity. cont. page 2

2 May 2002 Cell-specific Genomics (cont. from page 1) Cell-specific molecular analysis of small cell populations captured by LCM typically requires isolation of RNA and amplification of the isolated amounts of RNA from nanograms to micrograms. Thus, the combination of LCM, RNA isolation & amplification, and microarray analysis or quantitative PCR reveals differential gene expression that might otherwise be obscured in whole tissue samples. Arcturus provides a complete range of dedicated products for preparing and staining of sections, for isolating DNA and RNA from captured cells and for linear amplification of the isolated RNA. RNA from only a few Cells The RiboAmp RNA Amplification Kit enables the production of large amounts of RNA from minimal starting quantities for use in gene expression studies. Amplification using the RiboAmp Kit expands the number of experiments that can be conducted from precious samples. Based on a proprietary, patent pending, process, mrna is linear amplified up to 1000 fold in one synthesis round and up to one million fold in two rounds. The RiboAmp system produces antisense RNA (arna), singlestranded RNA of complementary nucleotide sequence to the original mrna species. Successful preparation of micrograms of arna from low-nanogram quantities of total RNA enables routine studies of small cell populations prepared by Laser Capture Microdissection. In addition, amplification of mrna in all abundance classes ensures that differential gene expression patterns will be identified at all levels of original copy numbers. The PicoPure DNA and RNA Isolation Kits are optimized reagent kits for isolation of DNA/RNA from Laser Capture microdissected cells. Superior recoveries of nucleic acids from as few as 10 cells are consistently seen. Total RNA isolated using the PicoPure RNA Kit is provided in as little as 10 µl of buffer ready for use in the RiboAmp RNA Amplification Kit to produce microgram quantities of RNA suitable for labeling and hybridization in downstream experiments. We would be glad to demonstrate the PixCell IIe at our facility in Basel, at your lab or at the new Arcturus training center near Frankfurt a.m., in Germany. For further information please use the reply card. Flexibility Speed Real time detection Four-color capability Expandability The Smart Cycler System Up to sixteen different cycling protocols can be performed simultaneously in one processing block. Experiments can be started at different times, allowing several operators to use the instrument concurrently. Rapid, precise heating and cooling significantly reduces cycling protocol time. Each of the sixteen sites are monitored in real time, growth curves are displayed and confirmation occurs at defined thresholds. Each of the sixteen reaction sites has its own four channel fluorescent detection. Up to four targets can be detected simultaneously. Up to six processing blocks of sixteen reaction/detection chambers can be controlled and monitored by one system Rapid DNA/RNA Amplification (cont. from page 1) The Smart Cycler software enables single or multiple operators to define and simultaneously carry out any number of separate experiments, each with a unique set of cycling protocols, threshold criteria, and data analysis. In addition, thermal and optical data from each and all sites can be monitored in real time, and graphs of temperature, growth curves, and melt curves can be charted as the data are collected. Six processing blocks of sixteen I- CORE modules can be controlled and monitored by one single PC. For a detailed description of the system please use the reply card or simply call us at

3 May 2002 PIPELINE A better way to dispense sterile fluids Essen Instrument's Pipeline was designed to replace tedious manual pipetting operations associated with high-volume cell culture protocols, such as in high throughput research applications. Pipeline provides a fast and accurate means for preparing multiple aliquots of a common reagent. Pipeline is also compatible with dispensing cells and suitable for use in cell culture. In multiplestep procedures, one can use an aspirator along with the Pipeline to exchange cell culture media, solutions and reagents without the need to change multiple pipettes. Two dispensing modes can be selected. The first mode dispenses a user-defined volume of fluid per switch activation, and the second mode dispenses continuously during switch activation. In addition, three different dispense speeds are possible allowing for the device to be tailored to the most demanding cell culture applications. The user has the ability to save and recall up to 8 pre-programmed settings, and the unit can be activated via front panel switch or foot switch. The accuracy and precision of the dispensed volumes are similar to serological pipettes. The Pipeline also provides an RS-232 interface if automation or remote operation is required. Additional information is available via the reply card, via or by calling Interested in Cell Migration? Molecular Devices' new SPECTRAmax Gemini EM Microplate Fluorometer New SPECTRAmax Gemini EM Essen Instrument's Pipeline The highly successful family of Molecular Devices SPECTRAmax microplate readers is growing, again. The Gemini EM, combining the unique dual scanning monochromator technology with top and bottom reading capability further broadens the range of applications such as cell migration studies and other filter plate assays (e.g. Transwell experiments, chemotaxis etc.) In addition to cell-based assays, Gemini fluorescence readers optimally support homogenous fluorescence intensity measurements, time-resolved fluorescence and glow-type luminescence detection, at high sensitivity and high speed. We shall be glad to demonstrate the Gemini EM or any of the Molecular Devices absorbance readers at your lab. Simply call New Kinase Assay Kits using IMAP Technology Molecular Devices has further extend its range of high performance reagent kits. In addition to the -no wash- calcium release, drug resistance and membrane potential reagents for cell based screening, five kinase assay kits are now available. Proprietary IMAP technology developed at Molecular Devices is designed to evaluate enzyme activity of various protein kinases. Based on non-antibody fluorescence polarization (FP) detection method, phosphorylation of fluorescein-labeled peptide is monitored. The assays are a simple, homogeneous mix and read procedure that allows a fast and accurate determination of enzyme activity. The reagent kits incorporate kinase enzymes from Upstate Biotechnology, the leader in signal transduction. The principle of the IMAP technology is based on the high affinity binding of phosphate by immobilized trivalent metal ions on nanoparticles provided with the assay kits. This IMAP binding reagent complexes with phosphate groups on phosphopeptides generated in a kinase reaction resulting in change of molecular motion and increase of fluorescence polarization. Unlike antibodybased kinase assays, IMAP is applicable to a wide variety of kinases regardless of the substrate peptide sequences. Available IMAP Assay Kits: Phosphodiesterase (PDE), Agammaglobulin Tyrosine Kinase (akt), Serum- and Glucocorticoidinducible Kinase (SGK), p38 Kinase and MAPK-activated protein kinase 2 (MAPKAP2). Please request detailed information for the IMAP technology and the growing list of available assay kits, as well as Molecular Devices' Analyst multidetection systems includ. fluorescence polarization detection

4 May 2002 A new Twist in DNA Purification The MICROLAB Automated Vacuum System (AVS) The new Hamilton AVS is a total automation solution for high throughput vacuum separation procedures performed on MICROLAB pipetting workstations. The AVS is a complete, automated system requiring no manual intervention or manifold assembly, increasing throughput by eliminating the nonproductive steps of vacuum separation procedures. In addition, AVS offers real-time monitoring and maintenance of the vacuum level to ensure consistent flow and performance. The patented MICROLAB AVS is a product of advanced engineering, providing total system control, integration, and efficient automation. The new Gripper Tool for the Hamilton Star pipetting workstation allows the transport of any microplate onto - or off-the deck. Combined, the AVS and Gripper tool enable complete walk away solutions for Genomics applications. Solid Phase Extraction Automation: the AVS MICROLAB SPE Workstation Utilizing the new Automated Vacuum System the Microlab SPE is the first 96 well format vacuum manifold that allows hands-free solid phase extraction automation. No manual intervention or grippers slow down the process resulting in increased throughput with reduced complexity and lowered cost. The MICROLAB AVS moves to position the SPE plate for conditioning and elution steps. The time for static manifold assembly and disassembly is eliminated. And solvent waste is automatically evacuated for complete automation. Plate after plate is processed without supervision, in less time. The SPE workstation allows for preand post-extraction dilutions, internal standard or derivatizing agent addition, as well as for SPE. Please call us for detailed information ( ). Pyrosequencing Extends Genotyping System Capabilities New PSQ HS 96A and PSQ 96MA The new PSQ HS 96A (High Sensitivity) is a dedicated system for SNP and mutation analysis. It uses proven Pyrosequencing technology to enable real time detection of DNA sequencing events. Reliable chemistry and a simple, robust detection mechanism eliminate the need for gels, dyes or specific labels. A highly sensitive camera enables small amounts of sample DNA to be analyzed, typically 5-10µl of PCR product. Only small volumes of reagents and nucleotides are required, reducing the cost per accurate result. The system employs a 96-well plate format and is automatically fed, by a robot arm, with up to ten plates from the integrated stacker. The instrument analyzes up to 960 samples unattended per 2 hour run with a typical throughput of 4,500 samples in a working day, corresponding to a throughput of 30,000 polymorphisms in 24 hours using triplex multiplexing. New functionality include: simultaneous comparison of several pyrograms, automatic analysis of tri-and tetra allelic SNPs, automatic analysis of insertions/deletions (InDels), automatic analysis of multiple SNPs and multiplexing of polymorphisms such as point mutations, InDels and SNPs. Each polymorphism is analyzed using a separate sequencing primer. Also new: PSQ 96MA The PSQ 96MA System is a multi-application sequence analysis solution based on the original PSQ 96 hardware with new software and reagent kits that expand the range of applications. Internal control, provided by the sequence data, eliminates ambiguous results and the New PSQ HS 96A (High Sensitivity) need to reverify. Genetic variations can be analyzed with accuracy and confidence in a gel free operating environment. The results provide base sequence information, polymorphic position context and statistical analysis options. New capabilities include automatic multiplex genotyping of SNPs and other mutations, allele frequency quantification and sequencing of short-strand DNA templates, including cloned material. Please call us for detailed information ( ). Bucher Biotec AG Schützengraben 7 CH-4051 Basel Switzerland Phone +41 (0) Fax +41 (0) [email protected]

5 Arcturus INFO REQUEST CARD Please send me the following information: PixCell IIe LCM System RiboAmp RNA Amplification Kit PicoPure RNA Isolation Kit Cepheid SmartCycler with Real Time Detection Essen Instruments Pipeline Sterile Fluid Dispenser Molecular Devices Gemini EM Microplate Fluorometer SPECTRAmax Absorbance Readers IMAP Kinase Assay Kits Analyst Multidetection Systems Hamilton Microlab AVS for DNA Purification Microlab AVS for SPE Automation Complete info on Hamilton Liquid Handling Robots Pyrosequencing PSQ HS 96A SNP Analyzer PSQ 96MA Multiple Analyzer My interest is: short term ( 3 month) medium term (6 to 12 months) longer term for information only INFO REQUEST CARD Please send me the following information: Arcturus PixCell IIe LCM System RiboAmp RNA Amplification Kit PicoPure RNA Isolation Kit Cepheid SmartCycler with Real Time Detection Essen Instruments Pipeline Sterile Fluid Dispenser Molecular Devices Gemini EM Microplate Fluorometer SPECTRAmax Absorbance Readers IMAP Kinase Assay Kits Analyst Multidetection Systems Hamilton Microlab AVS for DNA Purification Microlab AVS for SPE Automation Complete info on Hamilton Liquid Handling Robots Pyrosequencing PSQ HS 96A SNP Analyzer PSQ 96MA Multiple Analyzer My interest is: short term ( 3 month) medium term (6 to 12 months) longer term for information only

6 Absender: A Nicht frankieren Ne pas affranchir Non affrancare Geschäftsantwortsendung Invio commerciale-risposta Correspondance commerciale-réponse Bucher Biotec AG Schützengraben Basel Absender: Name/Titel: Name/Title: Firma/Universität: Company/University: Abteilung/Institut: Dept./Institute: Strasse/Nr.: Street/No: PLZ/Ort: Zip Code/City: Tel. Nr.: Phone: A Nicht frankieren Ne pas affranchir Non affrancare Geschäftsantwortsendung Invio commerciale-risposta Correspondance commerciale-réponse Bucher Biotec AG Schützengraben Basel Name/Titel: Name/Title: Firma/Universität: Company/University: Abteilung/Institut: Dept./Institute: Strasse/Nr.: Street/No: PLZ/Ort: Zip Code/City: Tel. Nr.: Phone: