Real-time qpcr Assay Design Software
|
|
|
- Dale McGee
- 8 years ago
- Views:
Transcription
1 Real-time qpcr Assay Design Software Your Blueprint For Success Informational Guide 2199 South McDowell Blvd Petaluma, CA USA GENOME.1( ) fax
2 Your Blueprint for Success Real-time qpcr Assay Design Performed at the easy click of a button RealTimeDesign software from Biosearch Technologies, Inc. is a free, easy to use, yet powerful assay design application for real-time quantitative PCR (real-time qpcr). Good probe and primer design is at the heart of any successful real-time qpcr assay, and being equipped with RealTimeDesign software, you re only a few clicks away from: Designing assays for SNP genotyping (2 probes and 2 primers) and for DNA/RNA quantification (1 probe and 2 primers). Designing Dual Labeled BHQ and BHQ probes and matching optimal primer pairs. Designing anywhere from single assays to high-throughput batches (design up to 10 different assays simultaneously) Saving a list of your custom designs in your account Design Run History (up to 100 designs) Choosing from Biosearch s wide selection of dyes including our very own BHQ (Black Hole Quencher ) dye Ordering your designed probes and primers directly through the Biosearch website: Good probe and primer design is at the heart of any successful real-time qpcr assay...
3 Real-time qpcr Assay Design Software, for both the Novice and Seasoned Expert Upon designing your probes and primers, RealTimeDesign software performs sophisticated algorithms to select an optimal sequence from your target of interest. What was once considered as an arduous, time-consuming task usually performed through a series of trial and error, qpcr researchers can now efficiently design and order assays during one swift online session. For more advanced users, RealTimeDesign software offers a Custom Mode allowing a fine control over a number of standard and customizable design parameters. Many qpcr experts put RealTimeDesign software to the test and they agree that this is one of the most useful and easy-to-use online design program available at no cost to the user. Determined to remain at the forefront of qpcr assay design, Biosearch constantly updates RealTimeDesign software with innovative features and increased functionality to provide our customers a step up in assay development.
4 Your Blueprint for Success DNA Quantification & Gene Expression Analysis RealTimeDesign Software Ensures Robust Amplification and Detection With the Quantitative PCR module of RealTimeDesign software, probes can be designed for sensitive measurements of copy number. When designing probes to quantify DNA for copy number studies (gdna) or gene expression analysis (cdna), traditional dual-labeled probes quenched with the BHQ dye are more than sufficient. However, many difficult target sites are AT-rich, and so fortified BHQ probes are required to maintain an elevated melting temperature. BHQ probes provide more flexibility in design of assays for DNA quantification: these shorter probes can be accommodated into a greater variety of targets and with a wider range in base composition. Replicate PCR Reactions, all amplified using the same primer set, but detected with either a traditional FAM-BHQ1 probe (black) or a BHQ probe (blue). The amplification product is a region within the AT-rich hemolysin III gene of Bacillus anthracis. With the Quantitative PCR module of RealTimeDesign software, probes can be designed for sensitive measurements of copy number. Choose BHQ or BHQ probes partnered with the ideal fluorophore for the instrument optics in use Use NCBI gene symbols, RefSeq IDs, or GenBank accession numbers, or use your own optimized sequence Pick the optimal assays designed by RealTimeDesign software or choose alternate primers and probes using Custom Mode Order directly through the Biosearch website
5 Probe-Based SNP Genotyping RealTimeDesign for Allelic Discrimination Because the BHQ technology stabilizes probe hybridization to the template, it allows for the design of shorter probes that offer increased resistance against hybridizing to a mismatch. This makes BHQ probes ideal for genotyping applications such as screening, linkage associations, or fine-mapping studies. Designing optimal probes is simple with RealTimeDesign s SNP Genotyping module (found under qpcr - BHQ Probe > Application > SNP Genotyping). Scatterplot of a SNP assay targeting alleles of UGT2B15 using a FAM BHQ probe and a TET BHQ probe. Use NCBI SNP Reference ID (rs#), gene symbols or RefSeq IDs derived from a SNP database such as dbsnp as a starting point for design Choose the fluorophore that matches your instrument optics. Review the optimal assay proposed by RealTimeDesign software Order directly through the Biosearch website BHQ Probes - Fortified Probes Achieve Powerful Discrimination A duplex stabilizing technology is used to elevate the oligo Tm over traditional fluorescence-quenched probes Permits a shorter probe sequence allowing flexible and discriminating assay design. Relies on the proven performance of the Black Hole Quencher (BHQ) dyes, the industry standard in dark quenchers. Screenshot from RealTimeDesign software, displaying the oligo sequences of the genotyping assay targeting UGT2B15, a gene involved in the metabolism of certain drugs such as tamoxifon. Save Time and Money, Let RealTimeDesign Software do the Prep Work!
6 Your Blueprint for Success Advanced Features of RealTimeDesign Software RTD software offers sophisticated features for the qpcr expert. By providing powerful control over oligo sequences, RTD software is your genome-grade software engine. High-Throughput 10 different assays can be designed simultaneously. Simply enter all of your targets in FASTA format, or as comma-separated accession numbers. Design Archives Rely upon the Design Run History to store your newly proposed sequences. Up to 100 different assays can be recorded for later review, in a unique database for every user. Parameters for BHQ Probe-Quantitative PCR-Custom Customizing Parameters Adjustable parameters provide complete control over design. Values can be fine-tuned and then made default for your future designs. Method Click Manage Parameter Sets from the Main Menu; Clone one of the current sets and rename it; Click on its name to adjust the values within; Once done, click Save and return to Manage Parameters; Click the Use checkbox to make your custom set the default.
7 Custom DNA Synthesis Products & Pricing This is a sample list of our real-time qpcr oligonucleotides. Other synthesis scales and dye selections are also available. For Biosearch s complete panel of dye-quencher combinations, please visit: or call GENOME.1 Dual Labeled BHQ Probes BHQ Probes 5 Label 5 Ex/Em (nm) 3 Label Catalog # Synthesis Scale 5 Label 5 Ex/Em (nm) 3 Label Catalog # Synthesis Scale FAM ValuProbe (single RP-HPLC) 495 / 520 BHQ-1 DLO-RFB-5 FAM 495 / 520 BHQ-1 DLO-FBP-5 FAM (dual HPLC) 495 / 520 BHQ-1 DLO-FB nmol DLO-FBP-1 DLO-FB1-1 FAM 495 / 520 TAMRA DLO-FT nmol TET 521 / 536 BHQ-1 DLO-TBP-5 DLO-FT-1 DLO-TBP-1 TET 521 / 536 BHQ-1 DLO-TEB1-5 DLO-TEB1-1 TET 521 / 536 TAMRA DLO-TET-5 DLO-TET-1 CAL Fluor Gold 540 CAL Fluor Orange / 544 BHQ / 559 BHQ-1 DLO-GBP-5 DLO-GBP-1 DLO-CBP-5 CAL Fluor Gold 540 (replaces TET) 522 / 544 BHQ-1 DLO-CGB1-5 DLO-CGB1-1 CAL Fluor Red / 610 BHQ-2 DLO-CBP-1 DLO-RBP-5 HEX 535 / 556 BHQ-1 DLO-HB1-5 DLO-RBP-1 CAL Fluor Orange 560 (replaces VIC/HEX/JOE) DLO-HB / 559 BHQ-1 DLO-COB1-5 Quasar / 667 BHQ-2 DLO-QBP-5 DLO-QBP-1 DLO-COB1-1 TAMRA 557 / 583 BHQ-2 DLO-TB2-5 DLO-TB2-1 CAL Fluor Red 610 (replaces Texas Red dye) 590 / 610 BHQ-2 DLO-CAB2-5 DLO-CAB2-1 Quasar 670 (replaces Cy5 dye) 647 / 667 BHQ-2 DLO-Q6B2-5 DLO-Q6B2-1 Quasar / 705 BHQ-2 DLO-Q7B2-5 DLO-Q7B Biosearch Technologies, Inc. All rights reserved. Biosearch, the Biosearch Technologies logo, Black Hole Quencher, BHQ, BHQ, CAL Fluor, and Quasar are registered trademarks of Biosearch Technologies, Inc. RealTimeDesign and RTD are trademarks of Biosearch Technologies, Inc. Advancing Nucleic Acid Technology is a service mark of Biosearch Technologies, Inc. Biosearch Technologies is the holder of record of patent applications or patents for Black Hole Quencher (BHQ ), CAL Fluor, Quasar and BHQ-Plus probes. Commercial use of these technologies require a license from Biosearch. For further information, please contact Biosearch Technologies, Inc. at licensing@biosearchtech.com. BR
8 2199 South McDowell Blvd Petaluma, CA USA GENOME.1( ) fax
Introduction to Quantitative PCR
Introduction to Quantitative PCR Methods and Applications Guide Introduction to Quantitative PCR Methods and Applications Guide IN 70200 D US and Canada Orders: 800-227-9770 x3 Technical Service: 800-227-9770
Technical Note. Roche Applied Science. No. LC 18/2004. Assay Formats for Use in Real-Time PCR
Roche Applied Science Technical Note No. LC 18/2004 Purpose of this Note Assay Formats for Use in Real-Time PCR The LightCycler Instrument uses several detection channels to monitor the amplification of
Co Extra (GM and non GM supply chains: Their CO EXistence and TRAceability) Outcomes of Co Extra
GM and non GM supply chains: Their CO EXistence and TRAceability Outcomes of Co Extra Comparison of different real time PCR chemistries and their suitability for detection and quantification of genetically
Introduction To Real Time Quantitative PCR (qpcr)
Introduction To Real Time Quantitative PCR (qpcr) SABiosciences, A QIAGEN Company www.sabiosciences.com The Seminar Topics The advantages of qpcr versus conventional PCR Work flow & applications Factors
Essentials of Real Time PCR. About Sequence Detection Chemistries
Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected
ONLINE SUPPLEMENTAL MATERIAL. Allele-Specific Expression of Angiotensinogen in Human Subcutaneous Adipose Tissue
ONLINE SUPPLEMENTAL MATERIAL Allele-Specific Expression of Angiotensinogen in Human Subcutaneous Adipose Tissue Sungmi Park 1, Ko-Ting Lu 1, Xuebo Liu 1, Tapan K. Chatterjee 2, Steven M. Rudich 3, Neal
Pitfalls in qpcr. Primer and probe design and synthesis. Mary Span Eurogentec S.A.
Pitfalls in qpcr Primer and probe design and synthesis Mary Span Eurogentec S.A. Steps in qpcr assay Set up experiment statistical relevant # samples/experimental group controls Design and synthesis primers
Methods and Application Guide. Introduction to Quantitative PCR
Methods and Application Guide Introduction to Quantitative PCR Introduction to Quantitative PCR Methods and Application Guide Stratagene USA and Canada Order: 800-424-5444 x3 Technical Services: 800-894-1304
QPCR Applications using Stratagene s Mx Real-Time PCR Platform
QPCR Applications using Stratagene s Mx Real-Time PCR Platform Dan Schoeffner, Ph.D Field Applications Scientist Dan.Schoeffner@Stratagene.com Tech. Services 800-894-1304 Polymerase Chain Reaction Melt
RealStar HBV PCR Kit 1.0 11/2012
RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 For research use only! (RUO) Product No.: 201003 96 rxns INS-201000-GB-02 Store at -25 C... -15 C November 2012 altona Diagnostics GmbH Mörkenstraße
Real-time PCR: Understanding C t
APPLICATION NOTE Real-Time PCR Real-time PCR: Understanding C t Real-time PCR, also called quantitative PCR or qpcr, can provide a simple and elegant method for determining the amount of a target sequence
qpcr Technical Guide Detection Methods Primer and Probe Design Instrumentation Applications Guide
qpcr Technical Guide Detection Methods Primer and Probe Design Instrumentation Applications Guide Table of Contents qpcr Technical Guide Introduction... 1 Quantitative PCR: How does it work?... 2 qpcr
quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476
BioScience quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476 This method describes a Real-time semi-quantitative TaqMan PCR procedure for the determination of
Technical Note. Roche Applied Science. No. LC 19/2004. Color Compensation
Roche Applied Science Technical Note No. LC 19/2004 Purpose of this Note Color The LightCycler System is able to simultaneously detect and analyze more than one color in each capillary. Due to overlap
Getting Started Guide
Primer Express Software Version 3.0 Getting Started Guide Before You Begin Designing Primers and Probes for Quantification Assays Designing Primers and Probes for Allelic Discrimination Assays Ordering
SICKLE CELL ANEMIA & THE HEMOGLOBIN GENE TEACHER S GUIDE
AP Biology Date SICKLE CELL ANEMIA & THE HEMOGLOBIN GENE TEACHER S GUIDE LEARNING OBJECTIVES Students will gain an appreciation of the physical effects of sickle cell anemia, its prevalence in the population,
A guide to the analysis of KASP genotyping data using cluster plots
extraction sequencing genotyping extraction sequencing genotyping extraction sequencing genotyping extraction sequencing A guide to the analysis of KASP genotyping data using cluster plots Contents of
QuantStudio 3D AnalysisSuite Software
USER GUIDE QuantStudio 3D AnalysisSuite Software for use with QuantStudio 3D Digital PCR System Publication Number MAN0008161 Revision 1.0 For Research Use Only. Not for use in diagnostic procedures. For
Factors Influencing Multiplex Real-Time PCR
APPLICATION NOTE Multiplex Real-Time PCR Factors Influencing Multiplex Real-Time PCR Introduction Multiplex PCR is the simultaneous amplification of more than one target sequence in a single reaction [1].
Reagent Guide. Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems
Reagent Guide Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Reagent Guide Copyright 2008, 2010 Applied Biosystems. All
Exercises for the UCSC Genome Browser Introduction
Exercises for the UCSC Genome Browser Introduction 1) Find out if the mouse Brca1 gene has non-synonymous SNPs, color them blue, and get external data about a codon-changing SNP. Skills: basic text search;
LightCycler 480 Real-Time PCR System
Roche Applied Science LightCycler 480 Real-Time PCR System Planned introduction of the LightCycler 480 System: September 2005 Rapid by nature, accurate by design The LightCycler 480 Real-Time PCR System
Absolute Quantification Getting Started Guide
5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide Introduction
PrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID papillary renal cell carcinoma (translocation-associated) PRCC Human This gene
Real-Time PCR Vs. Traditional PCR
Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives
Custom TaqMan Assays For New SNP Genotyping and Gene Expression Assays. Design and Ordering Guide
Custom TaqMan Assays For New SNP Genotyping and Gene Expression Assays Design and Ordering Guide For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in
Single Nucleotide Polymorphisms (SNPs)
Single Nucleotide Polymorphisms (SNPs) Additional Markers 13 core STR loci Obtain further information from additional markers: Y STRs Separating male samples Mitochondrial DNA Working with extremely degraded
TaqMan Mutation Detection Assay
PROTOCOL TaqMan Mutation Detection Assay Competitive Allele-Specific TaqMan PCR Publication Part Number 4467011 Rev. B Revision Date April 2012 For Research Use Only. Not intended for any animal or human
Quantification of Mycobacterium Tuberculosis. 150 tests
Quantification of Mycobacterium Tuberculosis rep 13E12 For general laboratory and research use only 150 tests Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis is the bacterium that
Applications Guide. Real-Time PCR Applications Guide
Applications Guide Real-Time PCR Applications Guide table of contents Table of Contents 1. Overview of Real-Time PCR 2 1.1 Key Concepts of Real-Time PCR 2 1.1.1 What Is Real-Time PCR? 2 1.1.2 How Real-Time
Oligonucleotide Stability Study
Oligonucleotide Stability Study IDT is performing a long term study of oligo stability. The study has been ongoing for 2 years and has tested oligo stability under various conditions. An overview of the
Plexor Systems Instrument Setup and Data Analysis for the Roche LightCycler 2.0 and LightCycler 1.5 Systems using LightCycler Software Version 4.
TECHNICAL MANUAL Plexor Systems Instrument Setup and Data Analysis for the Roche LightCycler 2.0 and LightCycler 1.5 Systems using LightCycler Software Version 4.0 Instruc ons for use of Products A4011,
Quando si parla di PCR quantitativa si intende:
Quando si parla di PCR quantitativa si intende: A. Una PCR che produce grandi quantità di DNA B. Una PCR che emette quanti di luce C. Una PCR che quantifica il numero di molecole stampo presenti all inizio
TruSeq Custom Amplicon v1.5
Data Sheet: Targeted Resequencing TruSeq Custom Amplicon v1.5 A new and improved amplicon sequencing solution for interrogating custom regions of interest. Highlights Figure 1: TruSeq Custom Amplicon Workflow
User Bulletin All PCR Instruments
User Bulletin All PCR Instruments May 26, 2000 SUBJECT: Primer Express Version 1.5 and TaqMan MGB Probes for Allelic Discrimination Release of Primer Express Version 1.5 Introducing TaqMan MGB Probes In
Gene Expression Assay Performance Guaranteed With the TaqMan Assays QPCR Guarantee Program
WHITE PAPER TaqMan Assays QPCR Guarantee Program Gene Expression Assay Performance Guaranteed With the TaqMan Assays QPCR Guarantee Program Real-Time PCR for the Quantification of Gene Expression Real-time
CopyCaller Software. User Guide
CopyCaller Software User Guide CopyCaller Software User Guide Information in this document is subject to change without notice. For Research Use Only. Not for use in diagnostic procedures. APPLIED BIOSYSTEMS
Fluorescent dyes for use with the
Detection of Multiple Reporter Dyes in Real-time, On-line PCR Analysis with the LightCycler System Gregor Sagner, Cornelia Goldstein, and Rob van Miltenburg Roche Molecular Biochemicals, Penzberg, Germany
2.500 Threshold. 2.000 1000e - 001. Threshold. Exponential phase. Cycle Number
application note Real-Time PCR: Understanding C T Real-Time PCR: Understanding C T 4.500 3.500 1000e + 001 4.000 3.000 1000e + 000 3.500 2.500 Threshold 3.000 2.000 1000e - 001 Rn 2500 Rn 1500 Rn 2000
TaqMan Gene Expression Assays
Product Guide TaqMan Gene Expression Assay Products Product Guide TaqMan Gene Expression Assays Applied Biosystems TaqMan Gene Expression Assays Solutions Applied Biosystems offers the largest family of
Highly specific and sensitive quantitation
PRODUCT ULLETIN SYR Select Master Mix SYR Select Master Mix Highly specific and sensitive quantitation SYR Select Master Mix offers advanced performance at an affordable price. SYR Select Master Mix is
PrimeTime. qpcr Application Guide. Experimental Overview, Protocol, Troubleshooting Fourth Edition
PrimeTime qpcr Application Guide Experimental Overview, Protocol, Troubleshooting Fourth Edition qpcr Application Guide Experimental Overview, Protocol, Troubleshooting Fourth Edition Managing Editors
Quantitative PCR Systems
QUANTITATIVE PCR Quantitative PCR Systems Our QPCR systems meet your current research needs, offer flexibility for future research goals, and match your budget. HIGH-PERFORMANCE FULL-FEATURED FLEXIBLE
DNA Integrity Number (DIN) For the Assessment of Genomic DNA Samples in Real-Time Quantitative PCR (qpcr) Experiments
DNA Integrity Number () For the Assessment of Genomic DNA Samples in Real-Time Quantitative PCR (qpcr) Experiments Application Note Nucleic Acid Analysis Author Arunkumar Padmanaban Agilent Technologies,
Eco TM 48 Real Time PCR System. Fastest block-based qpcr. Four colour multiplexing. Supports all chemistries. Fast cycling protocols
A Bibby Scientific Company Eco TM 48 Real Time PCR System Fast cycling Fastest block-base HRM functionality Four colour multiplexing MIQE compliant Easy to use software Supports all chemistries Small footprint
PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR. Results Interpretation Guide
PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR Results Interpretation Guide Pathogen Detection Systems by Real Time PCR Microbial offers real time PCR based systems for the detection of pathogenic bacteria
Replacing TaqMan SNP Genotyping Assays that Fail Applied Biosystems Manufacturing Quality Control. Begin
User Bulletin TaqMan SNP Genotyping Assays May 2008 SUBJECT: Replacing TaqMan SNP Genotyping Assays that Fail Applied Biosystems Manufacturing Quality Control In This Bulletin Overview This user bulletin
SeqScape Software Version 2.5 Comprehensive Analysis Solution for Resequencing Applications
Product Bulletin Sequencing Software SeqScape Software Version 2.5 Comprehensive Analysis Solution for Resequencing Applications Comprehensive reference sequence handling Helps interpret the role of each
PreciseTM Whitepaper
Precise TM Whitepaper Introduction LIMITATIONS OF EXISTING RNA-SEQ METHODS Correctly designed gene expression studies require large numbers of samples, accurate results and low analysis costs. Analysis
Quantification of Hepatitis B Virus. Core Protein Region. 150 tests
Quantification of Hepatitis B Virus Core Protein Region For general laboratory and research use only 150 tests Introduction to Hepatitis B Virus Originally known as serum hepatitis, hepatitis B has only
Gene Expression Macro Version 1.1
Gene Expression Macro Version 1.1 Instructions Rev B 1 Bio-Rad Gene Expression Macro Users Guide 2004 Bio-Rad Laboratories Table of Contents: Introduction..................................... 3 Opening
Human Herpes Virus 1 (Herpes simplex type 1) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...
TM Primerdesign Ltd TM Primerdesign Ltd Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...
Plexor Systems Instrument Setup and Data Analysis for the Applied Biosystems 7300 and 7500 Real-Time PCR Systems
Technical Manual Plexor Systems Instrument Setup and Data Analysis for the Applied Biosystems 7300 and 7500 Real-Time PCR Systems INSTRUCTIONS FOR USE OF PRODUCTS A4011, A4021, A4031, A4041, A4051 AND
SEQUENCING. From Sample to Sequence-Ready
SEQUENCING From Sample to Sequence-Ready ACCESS ARRAY SYSTEM HIGH-QUALITY LIBRARIES, NOT ONCE, BUT EVERY TIME The highest-quality amplicons more sensitive, accurate, and specific Full support for all major
How many of you have checked out the web site on protein-dna interactions?
How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss
Optimizing and Analyzing Real-Time Assays on the SmartCycler II System
Optimizing and Analyzing Real-Time Assays on the SmartCycler II System Cepheid Technical Support Overview This technical note provides guidelines on transferring, optimizing, and evaluating real-time PCR
Mir-X mirna First-Strand Synthesis Kit User Manual
User Manual Mir-X mirna First-Strand Synthesis Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc.
TaqMan OpenArray Genotyping. Getting Started Guide
TaqMan OpenArray Genotyping Getting Started Guide For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice.
TaqMan Genotyper Software v1.0.1 TaqMan Genotyping Data Analysis Software
TaqMan Genotyper Software v1.0.1 TaqMan Genotyping Data Analysis Software March 2011 Product Overview TaqMan Genotyper Software TaqMan Genotyper Software Standalone data analysis software for Applied Biosystems
PN 100-6812 REV. A2 USER GUIDE. D3 Assay Design. Fluidigm Corporation. All rights reserved.
PN 100-6812 REV. A2 USER GUIDE D3 Assay Design Copyright 2013 Fluidigm Corporation. All rights reserved. Fluidigm, the Fluidigm logo, Access Array, BioMark, C 1, D3 Assay Design, DELTAgene, Dynamic Array,
Real-time PCR handbook
Real-time PCR handbook Single-tube assays 96- and 384-well plates 384-well TaqMan Array cards OpenArray plates The image on this cover is of an OpenArray plate which is primarily used for mid-density real-time
CFX96 Real-Time PCR Detection System. Real-Time PCR: CFX96 System
Real-Time R Detection System Real-Time R: System Life Science Group Bio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55
amplification tech Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye
amplification tech note 6047 Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye Liz Jordan and Richard Kurtz, Gene Expression Division, io-rad
KASP genotyping chemistry User guide and manual
KASP genotyping chemistry User guide and manual KASP manual content 1. Introduction 3 2. Principal of the KASP genotyping assay 3 2.1 Mechanism of KASP chemistry 4 3. The KASP reaction component 5 3.1
Epstein Barr Virus (Human Herpes virus 4) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...
TM Primerdesign Ltd TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...
Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit
Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to
Critical Factors for Successful Real-Time PCR
Critical Factors for Successful Real-Time PCR Sample & ssay Technologies Contents 1. Introduction 4 2. Detection of PCR products in real-time 5 2.1 SYR Green 5 2.2 Fluorescently labeled sequence-specific
TaqMan Fast Advanced Master Mix. Protocol
TaqMan Fast Advanced Master Mix Protocol For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED
User Manual. Reference Gene Panel Mouse Probe protocol. Version 1.1 August 2014 For use in quantitative real-time PCR
User Manual Reference Gene Panel Mouse Probe protocol Version 1.1 August 2014 For use in quantitative real-time PCR Reference Gene Panel Mouse Table of contents Background 4 Assays included in the panel
DNA Detection. Chapter 13
DNA Detection Chapter 13 Detecting DNA molecules Once you have your DNA separated by size Now you need to be able to visualize the DNA on the gel somehow Original techniques: Radioactive label, silver
Brilliant QPCR Master Mix
Brilliant QPCR Master Mix Instruction Manual Catalog #600549 (single kit) #929549 (10-pack kit) Revision C Research Use Only. Not for Use in Diagnostic Procedures. 600549-12 LIMITED PRODUCT WARRANTY This
Scott Reierstad. Field Applications Scientist
Real-time PCR Training Scott Reierstad Field Applications Scientist Topics we ll cover Quick introduction to real-time PCR ABI-supported real-time chemistries Instrumentation Quantitation via Standard
Real Time PCR Kit for Human Herpes Virus Type 6 Cat No. TM-60027: Primer Set
780 Dubuque Avenue So. San Francisco, CA 94080, U.S.A. Tel: (800) 989-6296 / Fax:(650)871-2857 http://www.maximbio.com E-mail: mbi@maximbio.com Real Time PCR Kit for Human Herpes Virus Type 6 Cat No. TM-60027:
Hepatitis B Virus Genemer Mix
Product Manual Hepatitis B Virus Genemer Mix Primer Pair for amplification of HBV Specific DNA Fragment Includes Internal Negative Control Primers and Template Catalog No.: 60-2007-12 Store at 20 o C For
Bioflexible. Unleash superior performance with custom oligos and peptides from Sigma.
DNA Oligonucleotides qpcr Probes MISSION sirna Peptide Libraries ecommerce Solutions Bioflexible. Unleash superior performance with custom oligos and peptides from Sigma. bionucleics GYB_75596_Custom Capabilities
PrimePCR Assay Validation Report
Gene Information Gene Name sorbin and SH3 domain containing 2 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID SORBS2 Human Arg and c-abl represent the mammalian
RealLine HCV PCR Qualitative - Uni-Format
Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013
Nucleic Acid Amplification Life Science Dashboard Series 3
Brochure More information from http://www.researchandmarkets.com/reports/1937244/ Nucleic Acid Amplification Life Science Dashboard Series 3 Description: Nucleic acid amplification is one of the most commonly
Genomic DNA detection assay
Genomic DNA detection assay Detection of genomic DNA by real-time PCR Contents CTRL Internal controls and gdna detection Contents Kit Contents 3 Reagents and Equipment to Be Supplied by User 3 Kit Storage
A Primer of Genome Science THIRD
A Primer of Genome Science THIRD EDITION GREG GIBSON-SPENCER V. MUSE North Carolina State University Sinauer Associates, Inc. Publishers Sunderland, Massachusetts USA Contents Preface xi 1 Genome Projects:
BacReady TM Multiplex PCR System
BacReady TM Multiplex PCR System Technical Manual No. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI Detailed Experimental
QuantiFast Multiplex PCR Handbook
May 2011 QuantiFast Multiplex PCR Handbook QuantiFast Multiplex PCR Kit with master mix containing ROX passive reference dye QuantiFast Multiplex PCR +R Kit with separate tube of ROX passive reference
REAL-TIME PCR: Put the odds in your favor with SuperScript RT. FROM THEORY TO PRACTICE
i REAL-TIME PCR: FROM THEORY TO PRACTICE ii Put the odds in your favor with SuperScript RT. Engineered to be RNase H and incredibly thermostable, SuperScript III RT delivers robust first-strand synthesis
Creating a Color Compensation file for Roche LightCycler 480 vers.i & vers. II
Creating a Color Compensation file for Roche LightCycler 480 vers.i & vers. II Due to ROX and Cy5 dye emmision spectra overlap Roche LightCycler 480 can pick up signals from a dye measured by another channel.
Basic Analysis of Microarray Data
Basic Analysis of Microarray Data A User Guide and Tutorial Scott A. Ness, Ph.D. Co-Director, Keck-UNM Genomics Resource and Dept. of Molecular Genetics and Microbiology University of New Mexico HSC Tel.
Vector NTI Advance 11 Quick Start Guide
Vector NTI Advance 11 Quick Start Guide Catalog no. 12605050, 12605099, 12605103 Version 11.0 December 15, 2008 12605022 Published by: Invitrogen Corporation 5791 Van Allen Way Carlsbad, CA 92008 U.S.A.
StepOne and StepOnePlus Real-Time PCR Systems. Remarkably Simple Systems. Simply Remarkable Results.
StepOne and StepOnePlus Real-Time PCR Systems Remarkably Simple Systems. Simply Remarkable Results. Step Up to High-Performance Real-Time PCR Remarkably Simple Systems Applied Biosystems, a leader in technology
RT 2 Profiler PCR Array: Web-Based Data Analysis Tutorial
RT 2 Profiler PCR Array: Web-Based Data Analysis Tutorial Samuel J. Rulli, Jr., Ph.D. qpcr-applications Scientist Samuel.Rulli@QIAGEN.com Pathway Focused Research from Sample Prep to Data Analysis! -2-
StepOne and StepOnePlus Real-Time PCR Systems
PRODUCT BROCHURE Real-Time PCR Systems StepOne and StepOnePlus Real-Time PCR Systems Remarkably Simple Systems. Simply Remarkable Results. Step Up To High Performance Real-Time PCR Remarkably Simple Systems
Application Guide... 2
Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue Application Guide... 2 I. Description... 2 II. Product Components... 2 III. Materials to be Supplied
QuantStudio 3D AnalysisSuite Software: Relative Quantification
HELP QuantStudio 3D AnalysisSuite Software: Relative Quantification for use with QuantStudio 3D Digital PCR System and QuantStudio 3D AnalysisSuite Server System Publication Number MAN0009636 Revision
CUSTOM DNA SEQUENCING SERVICES
CUSTOM DNA SEQUENCING SERVICES Satisfied Customers are our Driving Force We never stop exceeding your Expectations Value Read Service Single read sequencing of plasmid inserts or PCR products in tube and
AS4.1 190509 Replaces 260806 Page 1 of 50 ATF. Software for. DNA Sequencing. Operators Manual. Assign-ATF is intended for Research Use Only (RUO):
Replaces 260806 Page 1 of 50 ATF Software for DNA Sequencing Operators Manual Replaces 260806 Page 2 of 50 1 About ATF...5 1.1 Compatibility...5 1.1.1 Computer Operator Systems...5 1.1.2 DNA Sequencing
The Power of Next-Generation Sequencing in Your Hands On the Path towards Diagnostics
The Power of Next-Generation Sequencing in Your Hands On the Path towards Diagnostics The GS Junior System The Power of Next-Generation Sequencing on Your Benchtop Proven technology: Uses the same long
mircute mirna qpcr Detection Kit (SYBR Green)
mircute mirna qpcr Detection Kit (SYBR Green) For detection of mirna using real-time RT-PCR (SYBR Green I) www.tiangen.com QP110302 mircute mirna qpcr Detection Kit (SYBR Green) Kit Contents Cat. no. FP401
The AB7900Fast and Principles of Real Time PCR
The AB7900Fast and Principles of Real Time PCR Michael Tavaria, PhD Scientific Applications Specialist, Applied Biosystems, Australia and New Zealand Outline Chemistries 7900Fast Real Time PCR System Applications
Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests.
TM Primerdesign Ltd TM Primerdesign Ltd Hepatitis B Virus Core Protein Region genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use
DNA Sequence Analysis
DNA Sequence Analysis Two general kinds of analysis Screen for one of a set of known sequences Determine the sequence even if it is novel Screening for a known sequence usually involves an oligonucleotide
Real-time quantitative RT -PCR (Taqman)
Real-time quantitative RT -PCR (Taqman) Author: SC, Patti Lab, 3/03 This is performed as a 2-step reaction: 1. cdna synthesis from DNase 1-treated total RNA 2. PCR 1. cdna synthesis (Advantage RT-for-PCR
Supplemental Material. Methods
Supplemental Material Methods Measurement of lncrnas expression Total RNA was extracted from PAXgene TM tubes using the PAXgene blood RNA kit (Qiagen, Venlo, Netherlands) as described by the manufacturer.