NEXT GENERATION SEQUENCING
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1 NEXT GENERATION SEQUENCING Dr. R. Piazza
2 SANGER SEQUENCING + DNA
3 NEXT GENERATION SEQUENCING Flowcell
4 NEXT GENERATION SEQUENCING Library di DNA Genomic DNA
5
6 NEXT GENERATION SEQUENCING
7 NEXT GENERATION SEQUENCING I 4 nucleotidi marcati con fluorocromi e bloccati in 3 sono aggiunti contemporaneamente Primer di sequenziamento Nucleotidi marcati e bloccati
8 NEXT GENERATION SEQUENCING ACQUISIZIONE DELL IMMAGINE RIMOZIONE DEL BLOCCO AL 3 RIMOZIONE DEL FLUOROFORO
9 HIGH-THROUGHPUT SEQUENCING
10 NEXT GENERATION SEQUENCING
11
12 HiSeq Lane/Flowcell 2 FLOWCELL 8 Lane/Flowcell 250 * 10^6 Cluster/Lane 250 * 10^6 Cluster/Lane 125bp 125bp 2 x 125bp/Cluster (Pair-end) Throughput = 2 * 8 * 250 * 10^6 * 2 * 125bp = bp!! = 1 Tb (Terabase)
13 SANGER SEQ vs. NGS THROUGHPUT COSTO PER-BASE Allele #1 Allele #2 C A G C G A C A G C A G C A T T G G G A C C A G C G A C A G C G G C A T T G G G A C Coverage = 5 NGS Read #5 NGS Read #4 NGS Read #3 NGS Read #2 NGS Read #1 Allele #1 Allele #2 C A G C G A C A G C G G C A T T G G G A C C A G C G A C A G C A G C A T T G G G A C C A G C G A C A G C A G C A T T G G G A C C A G C G A C A G C A G C A T T G G G A C C A G C G A C A G C G G C A T T G G G A C C A G C G A C A G C A G C A T T G G G A C C A G C G A C A G C G G C A T T G G G A C
14 HIGH-THROUGHPUT SEQUENCING: APPLICAZIONI DNA RNA GENOMIC DNA SEQUENCING RESEQUENCING DE NOVO SEQUENCING WHOLE-EXOME SEQUENCING ChIP-Seq ULTRADEEP SEQUENCING METHYL-SEQ mrna SEQUENCING TRANSCRIPTOME SEQUENCING (RNA-SEQ) TAG SEQUENCING (DITAG) MICRO-RNA STUDIES
15 WHOLE-GENOME, WHOLE-EXOME AND ULTRADEEP-SEQUENCING COVERAGE COVERAGE WHOLE-GENOME ULTRADEEP-SEQ
16 ULTRADEEP SEQUENCING QUANDO? M M ABL kinase domain
17 COVERAGE ULTRADEEP-SEQ
18 WHOLE-EXOME SEQUENCING
19
20
21 VARIANT CALLING SINGLE NUCLEOTIDE POLYMORPHISM VARIANT C T A A G G C T A A G T G C T A A G... G A G A G A T C T G A A T T G C T T T G C T A T T G C T G A T..ACTGAATTGCTGATTGTCAAGTCTGCTAGCG... MUTATION, SEQ ERROR OR SNP? CASE SAMPLE A A G G G G T T T T T T.....ACTGAATTGCTGATTGTCAAGTCTGCTAGCG.. CONTROL SAMPLE VarScan 2 ( Koboldt DC et al., Genome Res Mar;22(3):568-76
22 CASE CONTROL WHOLE-EXOME SEQUENCING GOES DIGITAL
23 CASE CONTROL LOSS OF HETEROZYGOSITY ALLELIC IMBALANCE A A T T A A T
24 WHOLE-EXOME SEQUENCING GOES DIGITAL: CEQer COMPARATIVE EXONIC QUANTIFICATION ANALYZER Piazza R. et al., PLoS One Oct 4;8(10):e74825
25 Statistical module Wilcoxon Signed-Rank test Test statistic W W N r ( case) ( control) sgn x x Ri i i i 1 As sample size increases (Nr> 10) the Z-Score converges to a Gaussian distribution! Estimating the error function of the normal distribution of W.. Wilcoxon Signed-Rank test erf ( x) 1..using the Abramowitz and Stegun approximation equation a t a 1 2 t 2 a 3 t 3 a 4 t 4 a 5 t 5 e x 2
26 Log2 Ratio CML-BC PATIENT: CML001BC Chr9 CDKN2A (p16)
27 CML-BC PATIENT: CML004BC Chr17 p53
28 ANALISI DI PRODOTTI DI FUSIONE ONCOGENICI
29 ANALISI DI PRODOTTI DI FUSIONE ONCOGENICI FRAMMENTAZIONE?
30 RNA-seq DRIVER FUSION TRANSCRIPTS IDENTIFICATION Junction reads Bridge reads 76bp 76bp Piazza R. et al., Nucleic Acids Res Sep;40(16):e123
31 ALIGNMENT TO HUMAN GENOME SAM BAM EXOME DATASET CCDS / REFFLAT EXOME BUILDER ABNORMAL PAIRS ABNORMAL PAIRS SCANNER BCR ex14 ABL ex2 HALF- MAPPED PAIRS??? Genome PUTATIVE TRANSLOCATIONS SET (PTS) PREFILTERING ALGORITHM Read Quality Mapping Quality Homology Filter Threshold Filter N Filter FILTERED HALF-MAPPED PAIRS FILTERED PTS
32 FILTERED PTS JUNCTION FINDER Ex12 Ex13 Ex14 Ex2 Ex3 Ex4 BCR ABL 1 JUNCTIONS LIST FILTERED HALF-MAPPED PAIRS ALIGNMENT ALGORITHM Ex14 Ex2 BCR??? JUNCTION JUNCTION READ JUNCTION
33 JUNCTION READ FRAME ALGORITHM DIRECTION ALGORITHM RECIPROCAL TRANSLOCATION ALGORITHM 5 BCR ABL 3 5 ABL BCR 3
34 AML1-ETO t(8;21) BCR-ABL1 p190 t(9;22) BCR-ABL1 p210 e13a2 t(9;22) BCR-ABL1 p210 e14a2 t(9;22) CBFB-MYH11 inv(16) CEP110-FGFR1 t(8;9) EWSR1-ERG t(21;22) MLL-MLLT1 t(11;19) MLL-MLLT3 t(9;11) MLLT10-PICALM t(10;11) NCOA4-RET inv(10) NPM-ALK t(2;5)
35 RNA-Seq HIGH EXPRESSION LOW EXPRESSION RNA-SEQ GOES DIGITAL READ EXON RPKM = READS PER KBASE PER MILLION OF MAPPED READS TPM = TRANSCRIPTS PER MILLION TOPHAT ( CUFFLINKS ( Trapnell C, et al. Nat. Biotechnol. 2010;28:
36 HIGH-THROUGHPUT SEQUENCING: APPLICAZIONI DNA RNA GENOMIC DNA SEQUENCING RESEQUENCING DE NOVO SEQUENCING WHOLE-EXOME SEQUENCING ChIP-Seq DEEP SEQUENCING METHYL-SEQ mrna SEQUENCING TRANSCRIPTOME SEQUENCING (RNA-SEQ) TAG SEQUENCING (DITAG) MICRO-RNA STUDIES
37 METHYL-SEQ
38
39 NEXT GENERATION SEQUENCING CON STRUMENTI NGS POSSIAMO SEQUENZIARE INTERI GENOMI I PROTOCOLLI RELATIVI A BUONA PARTE DELLE APPLICAZIONI E STANDARDIZZATA MOLTI TOOL DI ANALISI SONO FREE E OPEN-SOURCE AD OGGI C E BUONA STANDARDIZZAZIONE ANCHE DEI FORMATI FILE SIAMO IN UNA SITUAZIONE IDEALE??
40 NEXT GENERATION SEQUENCING
41 HIGH-THROUGHPUT SEQUENCING: DOMANI PAZIENTE EMOCROMO ESAMI EMATOCHIMICI ESAMI COLTURALI ESAMI STRUMENTALI SEQUENZIAMENTO GENOMA
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