Application of MLVA-15 genotyping for typing of Brucella abortus isolates from India. Dr. Gita Kumari
|
|
|
- Lenard Jesse Bryant
- 7 years ago
- Views:
Transcription
1 Application of MLVA-15 genotyping for typing of Brucella abortus isolates from India Dr. Gita Kumari
2 Introduction Brucellosis is considered as the most wide spread zoonosis in the world and a True zoonosis Genus -Brucella -Gram negative bacteria Bovine brucellosis -B. abortus most widespread form in Cattle economic impact on livestock industry due to abortions, stillbirths, weight loss, decreased milk production, hazard to human health barriers to international trade of animals and their products (Corbel, 2006) Control and Eradication of infection - continuous surveillance and epidemiological trace-back
3 Trace-back Analysis Strain-specific identification is essential to know the source of infection Requires a suitable typing technique for identification at sub-species level
4 Molecular typing of Brucella Outer membrane protein typing Insertion sequence based typing Pulsed field gel electrophoresis Amplified fragment length polymorphism Random Amplified Polymorphic DNA analysis Repetitive sequences based typing approaches (ERIC-PCR, REP-PCR, BOX A1R-PCR, (GTG)5-PCR, etc.) Multilocus sequence-typing (MLST) Multilocus Variable-Number Tandem- Repeat Analysis (MLVA) Species-specific polymorphisms Limited subspecies level diversity -do- -do- Sensitive to environmental conditions Sensitive to environmental conditions Global epidemiology of bacteria Local epidemiology and epidemiological trace-back in outbreak cases
5 Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Tandemly repeated sequences observed throughout the prokaryotic and eukaryotic genomes in thousands of copies (Bennett, 2000; van Belkum et al., 1999) Minisatellites:repeatunitsizesof9bporgreater Microsatellites:repeatunitsizesofupto8bp (Vergnaud and Pourcel. 2006) Combinations of minisatellite and microsatellite repeats in MLVA proven highly discriminatory in subtyping of monomorphic bacterial species, like Brucella (Bricker et al., 2003; Whatmoreet al., 2006; Le Fleche et al., 2006) High-speed molecular clocks (van Belkum, 1999).
6 MLVA contd. PCR-based Multipleallelescanbepresentatasinglelocus Size differences could be easily resolved by electrophoresis (Lindstedt, 2005; Vergnaud and Pourcel, 2006) When multiple loci are analyzed, the resulting fingerprint can be highly discriminatory or even unique. Use of multiple loci avoids dangers of incorrect conclusions being drawn from single loci Data can be easily stored and compared between laboratories leading to the development of International databases accessible via the Internet.
7 MATERIALS & METHODS Field isolates of B. abortus- 13 Reference strains- 4 Sl. Reference No. Strains Species-biovar Acc. No B. abortus bv 1 ATCC 23448/NCTC S19 B. Abortus NCTC S99 B. abortus R B. abortus B. melitensis 16M- Standard strain
8 S. N. Field Isolates Of Brucellaabortus Sample No./ Strain Used For The Present Study Species-biovar Host Occupation Source of isolation Year of isolation 1 12/02_VPH Brucella abortus M06_VPH Brucella abortus 2006 Place 3 07/VPH Brucella abortus Bovine Aborted fetus 2006 Kolkata 4 19/VPH Brucella abortus Bovine 2007 West Bengal 5 21/VPH Brucella abortus Bovine 2007 West Bengal 6 22/VPH Brucella abortus Bovine 2007 West Bengal 7 47a(8)/VPH Brucella abortus Cattle 2008 Bangalore 8 11/08_VPH Brucella abortus /VPH Brucella abortus Cattle 10 61/VPH Brucella abortus Cattle Aborted materials Aborted materials 2009 CADRAD 2009 CADRAD 11 75/VPH Brucella abortus Bovine Fetus 2010 Mizoram 12 76/VPH Brucella abortus Man Butcher Blood 2010 Maharashtra 13 BAB_VPH Brucella abortus
9 All the isolates were checked for purity & biochemical characteristics along with genus specific PCR (16S - 23S r-rna spacer gene) before use in the study Genus specific PCR Reaction mixture Cycle condition 10X PCR buffer 2.5 µl Initial denaturation 94 o C for 5 min dntp Name Forward Primer (5'-3') Reverse Primer (5'-3') References 16S-23S r-rna spacer genes 2.5 µl(2mm each) AAC ATA GAT CGC AGG CCA GTC AGC Denaturation 94 o C for 45 s MgCl2 2.5 µl(2.5 mm) Annealing 55 o C for 45 s TGC CAA TAT CCG TCT CAA GAC CAA M Kumar (2007) Forward primer 1 µl (10 pmol/µl) Extension 72 o C for 1 min Reverse primer 1 µl(10 pmol/µl) Final extension 72 o C for5 min 410bp Taq DNA polymerase 0.2 µl (1 unit) Cycles 30 Genomic DNA 3 µl NFW 12.8 µl Total Volume 25 µl Amplicon size: 410 bp
10 Oligonucleotide Primers used for MLVA Typing Name Forward Primer (5'-3') Reverse Primer (5'-3') References Panel 1 Minisatellite Loci Bruce 6 ATG GGA TGT GGT AGG GTA ATC G GCG TGA CAA TCG ACT TTT TGT C Bruce 8 ATT ATT CGC AGG CTC GTG ATT C ACA GAA GGT TTT CCA GCT CGT C Bruce 11 CTG TTG ATC TGA CCT TGC AAC C CCA GAC AAC AAC CTA CGT CCT G Bruce 12 CGG TAA ATC AAT TGT CCC ATG A GCC CAA GTT CAA CAG GAG TTT C Bruce 42 CAT CGC CTC AAC TAT ACC GTC A ACC GCA AAA TTT ACG CAT CG Bruce 43 TCT CAA GCC CGA TAT GGA GAA T TAT TTT CCG CCT GCC CAT AAA C MLVA-15 Bruce 45 ATC CTT GCC TCT CCC TAC CAG CGG GTA AAT ATC AAT GGC TTG G Le Fleche Bruce 55 TCA GGC TGT TTC GTC ATG TCT T AAT CTG GCG TTC GAG TTG TTC T et al. Panel 2 Microsatellite Loci (2006) Bruce 4 CTG ACG AAG GGA AGG CAA TAA G CGA TCT GGA GAT TAT CGG GAA G Bruce 7 GCT GAC GGG GAA GAACAT CTA T ACC CTT TTT CAG TCA AGG CAA A Bruce 9 GCG GAT TCG TTC TTCAGT TAT C GGG AGT ATG TTT TGG TTG TAC ATA G Bruce 16 ACG GGA GTT TTT GTT GCT CAA T GGC CAT GTT TCC GTT GAT TTA T Bruce 18 TAT GTT AGG GCA ATA GGG CAG T GAT GGT TGA GAG CAT TGT GAA G Bruce 21 CTC ATG CGC AAC CAA AAC A GAT TCG TGG TCG ATA ATC TCA TT Bruce 30 TGA CCG CAA AAC CAT ATC CTT C TATGTGCAGAGCTTCATGTTCG
11 MLVA-15Genotyping PCR reaction mix for 15 VNTR loci Sr. No. Bruce NFDW Glycerol (10%) 10x PCR Buffer 2 mm dntp Mix MgCl2 (25mM) Taq DNA Polymerase (5U/µl ) Forward Primer (10pm) Reverse Primer (10pm) Genomic DNA Total Reaction Volume µl 2.5µl µl µl µl µl 2.5µl µl µl µl µl µl 2.5 µl (Final conc. of 1X) 1.5 µl (Final conc. of 120µM of each) 1.5 µl (1.5mM) 0.2 µl (Final conc. 1U) 1 µl (Final conc. of 10pM) 1 µl (Final conc. of 10pM) 4 µl 25 µl µl µl µl µl µl
12 Amplification conditions for the 15 VNTR loci Sr. No. Bruce STEP1 Initial Denaturation 96 C for 5 min STEP2 Denaturation 96 C for 30 sec STEP3 Annealing 62⁰C for 30 sec 60⁰C for 30 sec ⁰C for 30 sec 8 55 STEP4 Extension 70⁰C for 1 min STEP5 Repeat (Steps 2 to 4) 30 cycles ⁰C for 30 sec 34 cycles ⁰C for 30 sec 30 cycles ⁰C for 30 sec ⁰C for 30 sec 30 cycles ⁰C for 30 sec 34 cycles 34 cycles Final Extension 70 C for 5 min Hold 4 C for Infinite
13 Analysis of PCR Products Done by Agarose Gel Electrophoresis Minisatellites(Panel 1 Loci) 2% (w/v) Agarose gel 100bp and 50bp DNA ladder constant 80V for 3 to 4 h depending on the particular VNTR loci. Microsatellites (Panel 2 Loci) 4% (w/v) High Resolution Agarose gel 20bp DNA ladder constant 80V for 6 h
14 Amplicon size determination and calculation of number of repeat at particular loci Comparing with standard strain s amplicon size (B. melitensis16m, ATCC 23456) 100bp DNA ladder 50bp DNA ladder 20bp DNA ladder Respective number of repeat at particular loci with the help of VNTR allelic Table
15
16 Repeat number present at each locus in standard strain (Brucella melitensis 16M, ATCC 23456) Sl. No. Locus VNTR Name PCR product size(bp) Tandem repeats (bp) Panel 1 1 Bruce06 BRU1322_134bp_408bp_3u Bruce08 BRU1134_18bp_348bp_4u Bruce11 BRU211_63bp_257bp_2u Bruce12 BRU73_15bp_392bp_13u Bruce42 BRU424_125bp_539bp_4u Bruce43 BRU379_12bp_182bp_2u Bruce45 BRU233_18bp_151bp_3u Bruce55 BRU2066_40bp_273bp_3u Panel 2 9 Bruce04 BRU1543_8bp_152bp_2u Bruce07 BRU1250_8bp_158bp_5u Bruce09 BRU588_8bp_156bp_7u Bruce16 BRU548_8bp_152bp_3u Bruce18 BRU339_8bp_146bp_5u Bruce21 BRU329_8bp_148bp_6u Bruce30 BRU1505_8bp_151bp_6u Unit size
17 Analysis of MLVA-15 genotyping START (Sequence Type Analysis and Recombinational Test) software, version (Jolleyet al.,2001) Allelic profile frequencies Allele frequencies Phylogenetic tree by UPGMA method Hunter-Gaston diversity index (HGDI) Measure of the variability of the TRs copy number at each locus. V-DICE (VNTR diversity and confidence extractor) (
18 Number of tandem repeats determined at each locus for B. abortus strains S.N. B. abortus strains /02_VPH M06_VPH /VPH /VPH /VPH /VPH a(8)/VPH /08_VPH /VPH /VPH /VPH /VPH BAB_VPH Ref Ref S Ref S Ref 1119-R
19 Allelic profile frequencies of B. abortus 13 different profile(s) in dataset (displayed in descending order of frequency) Genotype Profile ( Bruce 06, 08, 11, 12, 42, 43, 45, 55, 04, 07, 09, 16, 18, 21, 30) Frequency % of dataset A1 4, 5, 4, 12, 2, 2, 3, 3, 3, 4, 3, 3, 6, 8, A2 4, 5, 4, 12, 2, 2, 3, 3, 3, 4, 3, 3, 6, 8, A3 4, 5, 4, 12, 2, 2, 3, 3, 3, 6, 3, 3, 6, 8, A4 4, 5, 4, 12, 2, 2, 3, 3, 4, 4, 3, 3, 6, 8, A5 4, 5, 4, 12, 2, 2, 3, 3, 3, 5, 3, 3, 7, 8, A6 4, 5, 4, 12, 2, 2, 3, 3, 3, 4, 3, 3, 6, 8, A7 4, 5, 4, 12, 2, 2, 3, 3, 3, 4, 3, 3, 7, 8, A8 4, 5, 4, 13, 2, 2, 3, 3, 3, 4, 3, 3, 7, 8, A9 4, 5, 4, 12, 2, 2, 3, 3, 3, 4, 3, 3, 6, 8, A10 4, 5, 4, 12, 2, 2, 3, 3, 3, 5, 3, 3, 6, 8, A11 4, 5, 4, 12, 2, 2, 3, 3, 3, 5, 3, 4, 6, 8, A12 4, 5, 4, 12, 2, 2, 3, 3, 3, 5, 3, 4, 6, 8, A13 4, 5, 4, 12, 2, 2, 3, 3, 3, 6, 4, 3, 6, 8,
20 Allelic frequencies and Allelic Diversity of B. abortus Allele Total Allelic Diversity
21 Phylogenetic tree constructed from MLVA-15 fingerprinting of B.abortus strains using UPGMA 13 types observed out of 17 strains Isolates that clustered together had same place and year of isolation B. abortus, Ref 1119-R (4,5,4,12,2,2,3,3,3,6,4,3,6,8,5) B. abortus, Ref S 19 (4,5,4,12,2,2,3,3,3,6,3,3,6,8,5) B. abortus, Bab_VPH (4,5,4,12,2,2,3,3,3,6,3,3,6,8,5) B. abortus, Ref S99 (4,5,4,12,2,2,3,3,3,5,3,4,6,8,6) B. abortus, Ref 544 (4,5,4,12,2,2,3,3,3,5,3,4,6,8,5) B. abortus, 76/VPH,Maharashtra (4,5,4,12,2,2,3,3,3,5,3,3,6 B. abortus, 75/VPH,Mizoram (4,5,4,12,2,2,3,3,3,4,3,3,6,8,5 B. abortus, 21/VPH,West Bengal (4,5,4,12,2,2,3,3,3,4,3,3,6 B. abortus, 19/VPH,West Bengal (4,5,4,12,2,2,3,3,3,4,3,3,6 B. abortus, 07/VPH, West Bengal (4,5,4,12,2,2,3,3,3,4,3,3,6 B. abortus, 61/VPH,UP (4,5,4,12,2,2,3,3,3,4,3,3,6,8,6) B. abortus, 60/VPH,UP (4,5,4,12,2,2,3,3,3,4,3,3,6,8,6) B. abortus, 11/08_VPH (4,5,4,12,2,2,3,3,3,4,3,3,6,8,6) B. abortus, 12/02_VPH (4,5,4,12,2,2,3,3,4,4,3,3,6,8,6) B. abortus, 22/VPH,West Bengal (4,5,4,12,2,2,3,3,3,4,3,3,7 B. abortus, M06_VPH, Mahsrashtra (4,5,4,12,2,2,3,3,3,5,3, B. abortus, 47a(8)/VPH,Karnataka (4,5,4,13,2,2,3,3,3,4,3, Linkage Distance
22 Dendrogram showing comparison of Indian Brucella abortus strains with the foreign Brucella abortus strains( Same allelic profile of B. abortus,76/vph (Maharashtra ) and BCCN V1 B. abortusbiovar 1 United states) B. abortus, 75/VPH (Mizoram) and BCCN B.abortusbiovar 1 (Costa Rica) B.ab BAB_VPH, India (4,5,4,12,2,2,3,3,3,6,3,3,6,8,5) B.ab 76/VPH, India (4,5,4,12,2,2,3,3,3,5,3,3,6,8,5) BCCNV1,B.abo bv 1,United States (4,5,4,12,2,2,3,3,3,5,3,3,6,8,5) B.ab 75/VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,6,8,5) BCCN95-55,B.abo bv 1,Costa Rica (4,5,4,12,2,2,3,3,3,4,3,3,6,8,5) REF 292,B.abo bv 4,England (4,5,4,12,2,2,3,2,3,4,3,3,6,8,5) BCCN95-51,B.abo bv 1,Argentina (4,5,4,12,2,2,3,3,3,4,3,6,6,8,5) B.ab 61/VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,6,8,6) B.ab 60/VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,6,8,6) B.ab 11/08_VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,6,8,6) BfR97,B.abo bv 1,zimbabwe (4,5,4,12,2,2,3,1,3,4,3,3,6,8,6) B.ab 22/VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,7,8,7) B.ab 21/VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,6,8,7) B.ab 19/VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,6,8,7) B.ab 07/VPH, India (4,5,4,12,2,2,3,3,3,4,3,3,6,8,8) BfR96,B.abo bv 1,Zimbabwe (4,5,4,12,1,3,3,3,3,4,3,3,6,8,5) BfR91,B.abo bv 1,switzerland (4,5,4,12,2,3,3,3,3,4,3,3,6,8,5) REF86/8/59,B.abo bv 2,England (4,5,4,12,2,1,3,3,3,4,3,3,6,8,5) BCCNV5,B.abo United States (4,5,4,12,2,3,3,3,3,7,3,3,6,8,5) B.ab 47a(8)/VPH, India (4,5,4,13,2,2,3,3,3,4,3,3,7,8,5) B.ab M06_VPH, India (4,5,4,12,2,2,3,3,3,5,3,3,7,8,6) BfR99,B.abo bv 1,Switzerland (4,5,4,12,2,2,3,3,3,5,3,4,6,8,6) REF544,B.abo bv 1,England (4,5,4,12,2,2,3,3,3,5,3,4,5,8,5) B.ab 12/02_VPH, India (4,5,4,12,2,2,3,3,4,4,3,3,6,8,6) BCCN 96-62,B.abo bv 1,Italia (4,5,4,12,2,2,3,3,4,4,3,3,4,8,4) BCCN95-19,B.abo bv 1,France (4,5,4,12,2,2,3,3,4,4,3,3,6,8,4) BfR98,B.abo bv 1,Germany (4,5,4,12,2,2,3,3,5,4,3,6,6,8,4) BCCN 95-11,B.abo bv 1,France (3,5,4,12,2,2,3,3,5,7,3,4,6,8,4) BCCN 92-73,B.abo bv 1,France (3,5,4,12,2,2,3,3,5,6,3,4,6,8,4) BfR100,B.abo bv 1,Germany (4,5,4,12,1,2,3,3,5,4,3,4,6,6,5) BCCN93-26,B.abo bv 3,Sudan (3,5,4,11,2,2,3,3,6,8,3,7,6,8,7) REFTulya,B.abo bv 3,Uganda (3,5,4,11,2,2,3,3,6,5,3,11,8,8,5) BCCN93-15,B.abo bv 3,Spain (4,5,3,12,2,2,3,1,4,5,6,4,6,8,3) BCCN92-25,B.abo bv 3,France (4,5,3,12,2,2,3,1,4,5,5,4,6,8,3) BCCN91-90,B.abo bv 3,Greece (4,5,3,12,2,2,3,1,4,5,4,3,6,8,3) REF 870,B.abo bv 6,Africa (3,5,3,12,2,2,3,3,3,6,3,3,7,8,3) REF C68,B.abo bv 9,England (3,5,3,12,2,2,2,3,6,6,3,3,7,8,3) REF B3196,B.abo bv 5,England (3,5,3,12,2,2,2,3,6,7,3,3,7,8,3) Linkage Distance
23 17 Brucella abortusstrains (13 field isolates & 4 reference strains) were used in MLVA-15 genotyping MLVA-15 genotyping clearly discriminated Indian field isolates of Brucella abortus producing 13 genotypes The isolates that grouped together were of same source So, MLVA-15 genotyping sufficiently discriminated Indian field isolates of Brucella abortus. Specific grouping was also observed according to epidemiological data/source of infection
24
(http://genomes.urv.es/caical) TUTORIAL. (July 2006)
(http://genomes.urv.es/caical) TUTORIAL (July 2006) CAIcal manual 2 Table of contents Introduction... 3 Required inputs... 5 SECTION A Calculation of parameters... 8 SECTION B CAI calculation for FASTA
GENEWIZ, Inc. DNA Sequencing Service Details for USC Norris Comprehensive Cancer Center DNA Core
DNA Sequencing Services Pre-Mixed o Provide template and primer, mixed into the same tube* Pre-Defined o Provide template and primer in separate tubes* Custom o Full-service for samples with unknown concentration
DNA Sample preparation and Submission Guidelines
DNA Sample preparation and Submission Guidelines Requirements: Please submit samples in 1.5ml microcentrifuge tubes. Fill all the required information in the Eurofins DNA sequencing order form and send
10 µg lyophilized plasmid DNA (store lyophilized plasmid at 20 C)
TECHNICAL DATA SHEET BIOLUMINESCENCE RESONANCE ENERGY TRANSFER RENILLA LUCIFERASE FUSION PROTEIN EXPRESSION VECTOR Product: prluc-c Vectors Catalog number: Description: Amount: The prluc-c vectors contain
Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation
BDGP Resources Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation For recovery of sequences flanking PZ, PlacW and PEP elements E. Jay Rehm Berkeley Drosophila Genome Project I.
(A) Microarray analysis was performed on ATM and MDM isolated from 4 obese donors.
Legends of supplemental figures and tables Figure 1: Overview of study design and results. (A) Microarray analysis was performed on ATM and MDM isolated from 4 obese donors. After raw data gene expression
UNIVERSITETET I OSLO Det matematisk-naturvitenskapelige fakultet
1 UNIVERSITETET I OSLO Det matematisk-naturvitenskapelige fakultet Exam in: MBV4010 Arbeidsmetoder i molekylærbiologi og biokjemi I MBV4010 Methods in molecular biology and biochemistry I Day of exam:.
Table S1. Related to Figure 4
Table S1. Related to Figure 4 Final Diagnosis Age PMD Control Control 61 15 Control 67 6 Control 68 10 Control 49 15 AR-PD PD 62 15 PD 65 4 PD 52 18 PD 68 10 AR-PD cingulate cortex used for immunoblot
Supplementary Online Material for Morris et al. sirna-induced transcriptional gene
Supplementary Online Material for Morris et al. sirna-induced transcriptional gene silencing in human cells. Materials and Methods Lentiviral vector and sirnas. FIV vector pve-gfpwp was prepared as described
Mutations and Genetic Variability. 1. What is occurring in the diagram below?
Mutations and Genetic Variability 1. What is occurring in the diagram below? A. Sister chromatids are separating. B. Alleles are independently assorting. C. Genes are replicating. D. Segments of DNA are
pcas-guide System Validation in Genome Editing
pcas-guide System Validation in Genome Editing Tagging HSP60 with HA tag genome editing The latest tool in genome editing CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible
SERVICES CATALOGUE WITH SUBMISSION GUIDELINES
SERVICES CATALOGUE WITH SUBMISSION GUIDELINES 3921 Montgomery Road Cincinnati, Ohio 45212 513-841-2428 www.agctsequencing.com CONTENTS Welcome Dye Terminator Sequencing DNA Sequencing Services - Full Service
Next Generation Sequencing
Next Generation Sequencing 38. Informationsgespräch der Blutspendezentralefür Wien, Niederösterreich und Burgenland Österreichisches Rotes Kreuz 22. November 2014, Parkhotel Schönbrunn Die Zukunft hat
Introduction to Perl Programming Input/Output, Regular Expressions, String Manipulation. Beginning Perl, Chap 4 6. Example 1
Introduction to Perl Programming Input/Output, Regular Expressions, String Manipulation Beginning Perl, Chap 4 6 Example 1 #!/usr/bin/perl -w use strict; # version 1: my @nt = ('A', 'C', 'G', 'T'); for
Gene Synthesis 191. Mutagenesis 194. Gene Cloning 196. AccuGeneBlock Service 198. Gene Synthesis FAQs 201. User Protocol 204
Gene Synthesis 191 Mutagenesis 194 Gene Cloning 196 AccuGeneBlock Service 198 Gene Synthesis FAQs 201 User Protocol 204 Gene Synthesis Overview Gene synthesis is the most cost-effective way to enhance
The p53 MUTATION HANDBOOK
The p MUTATION HANDBOOK Version 1. /7 Thierry Soussi Christophe Béroud, Dalil Hamroun Jean Michel Rubio Nevado http://p/free.fr The p Mutation HandBook By T Soussi, J.M. Rubio-Nevado, D. Hamroun and C.
Molecular analyses of EGFR: mutation and amplification detection
Molecular analyses of EGFR: mutation and amplification detection Petra Nederlof, Moleculaire Pathologie NKI Amsterdam Henrique Ruijter, Ivon Tielen, Lucie Boerrigter, Aafke Ariaens Outline presentation
Hands on Simulation of Mutation
Hands on Simulation of Mutation Charlotte K. Omoto P.O. Box 644236 Washington State University Pullman, WA 99164-4236 omoto@wsu.edu ABSTRACT This exercise is a hands-on simulation of mutations and their
Supplementary Information. Binding region and interaction properties of sulfoquinovosylacylglycerol (SQAG) with human
Supplementary Information Binding region and interaction properties of sulfoquinovosylacylglycerol (SQAG) with human vascular endothelial growth factor 165 revealed by biosensor based assays Yoichi Takakusagi
Inverse PCR and Sequencing of P-element, piggybac and Minos Insertion Sites in the Drosophila Gene Disruption Project
Inverse PCR and Sequencing of P-element, piggybac and Minos Insertion Sites in the Drosophila Gene Disruption Project Protocol for recovery of sequences flanking insertions in the Drosophila Gene Disruption
pcmv6-neo Vector Application Guide Contents
pcmv6-neo Vector Application Guide Contents Package Contents and Storage Conditions... 2 Product Description... 2 Introduction... 2 Production and Quality Assurance... 2 Methods... 3 Other required reagents...
RT-PCR: Two-Step Protocol
RT-PCR: Two-Step Protocol We will provide both one-step and two-step protocols for RT-PCR. We recommend the twostep protocol for this class. In the one-step protocol, the components of RT and PCR are mixed
Y-chromosome haplotype distribution in Han Chinese populations and modern human origin in East Asians
Vol. 44 No. 3 SCIENCE IN CHINA (Series C) June 2001 Y-chromosome haplotype distribution in Han Chinese populations and modern human origin in East Asians KE Yuehai ( `º) 1, SU Bing (3 Á) 1 3, XIAO Junhua
NimbleGen SeqCap EZ Library SR User s Guide Version 3.0
NimbleGen SeqCap EZ Library SR User s Guide Version 3.0 For life science research only. Not for use in diagnostic procedures. Copyright 2011 Roche NimbleGen, Inc. All Rights Reserved. Editions Version
Title : Parallel DNA Synthesis : Two PCR product from one DNA template
Title : Parallel DNA Synthesis : Two PCR product from one DNA template Bhardwaj Vikash 1 and Sharma Kulbhushan 2 1 Email: vikashbhardwaj@ gmail.com 1 Current address: Government College Sector 14 Gurgaon,
ANALYSIS OF A CIRCULAR CODE MODEL
ANALYSIS OF A CIRCULAR CODE MODEL Jérôme Lacan and Chrstan J. Mchel * Laboratore d Informatque de Franche-Comté UNIVERSITE DE FRANCHE-COMTE IUT de Belfort-Montbélard 4 Place Tharradn - BP 747 5 Montbélard
Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR. Protocol
Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR Protocol 31 January 2011 Joint Research Centre Institute for Health and Consumer Protection Molecular Biology and Genomics
Gene Finding CMSC 423
Gene Finding CMSC 423 Finding Signals in DNA We just have a long string of A, C, G, Ts. How can we find the signals encoded in it? Suppose you encountered a language you didn t know. How would you decipher
ANALYSIS OF GROWTH HORMONE IN TENCH (TINCA TINCA) ANALÝZA RŮSTOVÉHO HORMONU LÍNA OBECNÉHO (TINCA TINCA)
ANALYSIS OF GROWTH HORMONE IN TENCH (TINCA TINCA) ANALÝZA RŮSTOVÉHO HORMONU LÍNA OBECNÉHO (TINCA TINCA) Zrůstová J., Bílek K., Baránek V., Knoll A. Ústav morfologie, fyziologie a genetiky zvířat, Agronomická
APOT - Assay. Protocol for HPV16 and 18. Amplification of Papilloma Virus Oncogene Transcripts HPV. E6 E7 E1 Zelluläre DNA poly(a)
E5 E2 E1 APOT - Assay Amplification of Papilloma Virus Oncogene Transcripts URR E6 E7 L1 HPV L2 E4 E6 E7 E1 Zelluläre DNA poly(a) Protocol for HPV16 and 18 Brief summary of the APOT assay Fig.1A shows
Supplemental Data. Short Article. PPARγ Activation Primes Human Monocytes. into Alternative M2 Macrophages. with Anti-inflammatory Properties
Cell Metabolism, Volume 6 Supplemental Data Short Article PPARγ Activation Primes Human Monocytes into Alternative M2 Macrophages with Anti-inflammatory Properties M. Amine Bouhlel, Bruno Derudas, Elena
Marine Biology DEC 2004; 146(1) : 53-64 http://dx.doi.org/10.1007/s00227-004-1423-6 Copyright 2004 Springer
Marine Biology DEC 2004; 146(1) : 53-64 http://dx.doi.org/10.1007/s00227-004-1423-6 Copyright 2004 Springer Archimer http://www.ifremer.fr/docelec/ Archive Institutionnelle de l Ifremer The original publication
Transmembrane Signaling in Chimeras of the E. coli Chemotaxis Receptors and Bacterial Class III Adenylyl Cyclases
Transmembrane Signaling in Chimeras of the E. coli Chemotaxis Receptors and Bacterial Class III Adenylyl Cyclases Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Eberhard Karls Universität
Heraeus Sepatech, Kendro Laboratory Products GmbH, Berlin. Becton Dickinson,Heidelberg. Biozym, Hessisch Oldendorf. Eppendorf, Hamburg
13 4. MATERIALS 4.1 Laboratory apparatus Biofuge A Centrifuge 5804R FACScan Gel electrophoresis chamber GPR Centrifuge Heraeus CO-AUTO-ZERO Light Cycler Microscope Motopipet Neubauer Cell Chamber PCR cycler
Chapter 9. Applications of probability. 9.1 The genetic code
Chapter 9 Applications of probability In this chapter we use the tools of elementary probability to investigate problems of several kinds. First, we study the language of life by focusing on the universal
DISSERTATIONES MEDICINAE UNIVERSITATIS TARTUENSIS 108
DISSERTATIONES MEDICINAE UNIVERSITATIS TARTUENSIS 108 DISSERTATIONES MEDICINAE UNIVERSITATIS TARTUENSIS 108 THE INTERLEUKIN-10 FAMILY CYTOKINES GENE POLYMORPHISMS IN PLAQUE PSORIASIS KÜLLI KINGO TARTU
Module 6: Digital DNA
Module 6: Digital DNA Representation and processing of digital information in the form of DNA is essential to life in all organisms, no matter how large or tiny. Computing tools and computational thinking
Five-minute cloning of Taq polymerase-amplified PCR products
TOPO TA Cloning Version R 8 April 2004 25-0184 TOPO TA Cloning Five-minute cloning of Taq polymerase-amplified PCR products Catalog nos. K4500-01, K4500-40, K4510-20, K4520-01, K4520-40, K4550-01, K4550-40,
Cloning, sequencing, and expression of H.a. YNRI and H.a. YNII, encoding nitrate and nitrite reductases in the yeast Hansenula anomala
Cloning, sequencing, and expression of H.a. YNRI and H.a. YNII, encoding nitrate and nitrite reductases in the yeast Hansenula anomala -'Pablo García-Lugo 1t, Celedonio González l, Germán Perdomo l, Nélida
Molecular typing of VTEC: from PFGE to NGS-based phylogeny
Molecular typing of VTEC: from PFGE to NGS-based phylogeny Valeria Michelacci 10th Annual Workshop of the National Reference Laboratories for E. coli in the EU Rome, November 5 th 2015 Molecular typing
Differentiation of Klebsiella pneumoniae and K. oxytoca by Multiplex Polymerase Chain Reaction
Differentiation of Klebsiella pneumoniae and K. oxytoca by Multiplex Polymerase Chain Reaction Yogesh Chander 1 M. A. Ramakrishnan 1 Naresh Jindal 1 Kevan Hanson 2 Sagar M. Goyal 1 1 Department of Veterinary
TITRATION OF raav (VG) USING QUANTITATIVE REAL TIME PCR
Page 1 of 5 Materials DNase digestion buffer [13 mm Tris-Cl, ph7,5 / 5 mm MgCl2 / 0,12 mm CaCl2] RSS plasmid ptr-uf11 SV40pA Forward primer (10µM) AGC AAT AGC ATC ACA AAT TTC ACA A SV40pA Reverse Primer
The making of The Genoma Music
242 Summary Key words Resumen Palabras clave The making of The Genoma Music Aurora Sánchez Sousa 1, Fernando Baquero 1 and Cesar Nombela 2 1 Department of Microbiology, Ramón y Cajal Hospital, and 2 Department
N-terminal Regulatory Domains of Phosphodiesterases 1, 4, 5 and 10 examined with an Adenylyl Cyclase as a Reporter
N-terminal Regulatory Domains of Phosphodiesterases 1, 4, 5 and 10 examined with an Adenylyl Cyclase as a Reporter Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Eberhard Karls Universität
Molecular chaperones involved in preprotein. targeting to plant organelles
Molecular chaperones involved in preprotein targeting to plant organelles Dissertation der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Christine Fellerer München 29.
Distribution of the DNA transposon family, Pokey in the Daphnia pulex species complex
Eagle and Crease Mobile DNA (2016) 7:11 DOI 10.1186/s13100-016-0067-7 RESEARCH Open Access Distribution of the DNA transposon family, Pokey in the Daphnia pulex species complex Shannon H. C. Eagle and
Thermo Scientific Phusion Site-Directed Mutagenesis Kit #F-541
PRODUCT INFORMATION Thermo Scientific Phusion Site-Directed Mutagenesis Kit #F-541 Lot _ Store at -20 C Expiry Date _ www.thermoscientific.com/onebio CERTIFICATE OF ANALYSIS The Phusion Site-Directed Mutagenesis
9. Materials. 9.1 Chemicals Acetic Acid (glacial) Materials. 8-Aminoguanosine Ammonium Hydroxide Ammonium Persulfate.
9. Materials 9.1 Chemicals Acetic Acid (glacial) Acetone Acetonitrile Agarose 8-Aminoguanosine Ammonium Hydroxide Ammonium Persulfate Ampicillin ATP Bromophenol Blue Butanol Chloroform CTP 7-Deaza-GTP
The DNA-"Wave Biocomputer"
The DNA-"Wave Biocomputer" Peter P. Gariaev (Pjotr Garjajev)*, Boris I. Birshtein*, Alexander M. Iarochenko*, Peter J. Marcer**, George G. Tertishny*, Katherine A. Leonova*, Uwe Kaempf ***. * Institute
All commonly-used expression vectors used in the Jia Lab contain the following multiple cloning site: BamHI EcoRI SmaI SalI XhoI_ NotI
2. Primer Design 2.1 Multiple Cloning Sites All commonly-used expression vectors used in the Jia Lab contain the following multiple cloning site: BamHI EcoRI SmaI SalI XhoI NotI XXX XXX GGA TCC CCG AAT
Supporting Information
Supporting Information Wiley-VCH 2007 69451 Weinheim, Germany Evolving a Thermostable DNA polymerase that Accurately Amplifies Highly-Damaged Templates Christian Gloeckner, Katharina B. M. Sauter, and
Association of IGF1 and IGFBP3 polymorphisms with colorectal polyps and colorectal cancer risk
DOI 10.1007/s10552-009-9438-4 ORIGINAL PAPER Association of IGF1 and IGFBP3 polymorphisms with colorectal polyps and colorectal cancer risk Elisabeth Feik Æ Andreas Baierl Æ Barbara Hieger Æ Gerhard Führlinger
Characterization of cdna clones of the family of trypsin/a-amylase inhibitors (CM-proteins) in barley {Hordeum vulgare L.)
Characterization of cdna clones of the family of trypsin/a-amylase inhibitors (CM-proteins) in barley {Hordeum vulgare L.) J. Paz-Ares, F. Ponz, P. Rodríguez-Palenzuela, A. Lázaro, C. Hernández-Lucas,
Drosophila NK-homeobox genes
Proc. Natl. Acad. Sci. USA Vol. 86, pp. 7716-7720, October 1989 Biochemistry Drosophila NK-homeobox genes (NK-1, NK-2,, and DNA clones/chromosome locations of genes) YONGSOK KIM AND MARSHALL NIRENBERG
Part ONE. a. Assuming each of the four bases occurs with equal probability, how many bits of information does a nucleotide contain?
Networked Systems, COMPGZ01, 2012 Answer TWO questions from Part ONE on the answer booklet containing lined writing paper, and answer ALL questions in Part TWO on the multiple-choice question answer sheet.
inhibition of mitosis
The EMBO Journal vol.13 no.2 pp.425-434, 1994 cdt 1 is an essential target of the Cdc 1 O/Sct 1 transcription factor: requirement for DNA replication and inhibition of mitosis Johannes F.X.Hofmann and
EU Reference Laboratory for E. coli Department of Veterinary Public Health and Food Safety Unit of Foodborne Zoonoses Istituto Superiore di Sanità
Identification and characterization of Verocytotoxin-producing Escherichia coli (VTEC) by Real Time PCR amplification of the main virulence genes and the genes associated with the serogroups mainly associated
Mutation. Mutation provides raw material to evolution. Different kinds of mutations have different effects
Mutation Mutation provides raw material to evolution Different kinds of mutations have different effects Mutational Processes Point mutation single nucleotide changes coding changes (missense mutations)
TA Cloning Kit. Version V 7 April 2004 25-0024. Catalog nos. K2000-01, K2000-40, K2020-20, K2020-40, K2030-01 K2030-40, K2040-01, K2040-40
TA Cloning Kit Version V 7 April 2004 25-0024 TA Cloning Kit Catalog nos. K2000-01, K2000-40, K2020-20, K2020-40, K2030-01 K2030-40, K2040-01, K2040-40 ii Table of Contents Table of Contents...iii Important
Insulin Receptor Gene Mutations in Iranian Patients with Type II Diabetes Mellitus
Iranian Biomedical Journal 13 (3): 161-168 (July 2009) Insulin Receptor Gene Mutations in Iranian Patients with Type II Diabetes Mellitus Bahram Kazemi 1*, Negar Seyed 1, Elham Moslemi 2, Mojgan Bandehpour
Six Homeoproteins and a Iinc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype
Six Homeoproteins and a Iinc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype Iori Sakakibara 1,2,3, Marc Santolini 4, Arnaud Ferry 2,5, Vincent Hakim 4, Pascal Maire 1,2,3 * 1 INSERM U1016,
Archimer http://archimer.ifremer.fr
Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site Fish
2006 7.012 Problem Set 3 KEY
2006 7.012 Problem Set 3 KEY Due before 5 PM on FRIDAY, October 13, 2006. Turn answers in to the box outside of 68-120. PLEASE WRITE YOUR ANSWERS ON THIS PRINTOUT. 1. Which reaction is catalyzed by each
Identification and characterisation of Verocytotoxinproducing Escherichia coli (VTEC) by PCR amplification of virulence genes
CRL_Method 01 28_04_2008 Pag 1 of 10 Identification and characterisation of Verocytotoxinproducing Escherichia coli (VTEC) by PCR amplification of virulence genes CRL_Method 01 28_04_2008 Pag 2 of 10 INDEX
Interleukin-4 Receptor Signal Transduction: Involvement of P62
Interleukin-4 Receptor Signal Transduction: Involvement of P62 Den Naturwissenschaftlichen Fakultäten der Friedrich Alexander Universität Erlangen Nürnberg zur Erlangung des Doktorgrades vorgelegt von
were demonstrated to be, respectively, the catalytic and regulatory subunits of protein phosphatase 2A (PP2A) (29).
JOURNAL OF VIROLOGY, Feb. 1992, p. 886-893 0022-538X/92/020886-08$02.00/0 Copyright C) 1992, American Society for Microbiology Vol. 66, No. 2 The Third Subunit of Protein Phosphatase 2A (PP2A), a 55- Kilodalton
Directed-Mutagenesis and Deletion Generated through an Improved Overlapping-Extension PCR Based Procedure
Research Article Directed-Mutagenesis and Deletion Generated through an Improved Overlapping-Extension PCR Based Procedure Wirojne Kanoksilapatham 1*, Juan M. González 2 and Frank T. Robb 3 1 Department
On Covert Data Communication Channels Employing DNA Recombinant and Mutagenesis-based Steganographic Techniques
On Covert Data Communication Channels Employing DNA Recombinant and Mutagenesis-based Steganographic Techniques MAGDY SAEB 1, EMAN EL-ABD 2, MOHAMED E. EL-ZANATY 1 1. School of Engineering, Computer Department,
Coding sequence the sequence of nucleotide bases on the DNA that are transcribed into RNA which are in turn translated into protein
Assignment 3 Michele Owens Vocabulary Gene: A sequence of DNA that instructs a cell to produce a particular protein Promoter a control sequence near the start of a gene Coding sequence the sequence of
Forensic DNA Testing Terminology
Forensic DNA Testing Terminology ABI 310 Genetic Analyzer a capillary electrophoresis instrument used by forensic DNA laboratories to separate short tandem repeat (STR) loci on the basis of their size.
Biopython Tutorial and Cookbook
Biopython Tutorial and Cookbook Jeff Chang, Brad Chapman, Iddo Friedberg, Thomas Hamelryck, Michiel de Hoon, Peter Cock Last Update September 2008 Contents 1 Introduction 5 1.1 What is Biopython?.........................................
The Arabinosyltransferase EmbC Is Inhibited by Ethambutol in Mycobacterium tuberculosis
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Oct. 2009, p. 4138 4146 Vol. 53, No. 10 0066-4804/09/$08.00 0 doi:10.1128/aac.00162-09 Copyright 2009, American Society for Microbiology. All Rights Reserved. The
DNA: A Person s Ultimate Fingerprint
A partnership between the UAB Center for Community Outreach Development and McWane Center DNA: A Person s Ultimate Fingerprint This project is supported by a Science Education Partnership Award (SEPA)
Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer s disease is this type 3 diabetes?
Journal of Alzheimer s Disease 7 (2005) 63 80 63 IOS Press Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer s disease is this type 3 diabetes? Eric Steen,
Molecular detection of Babesia rossi and Hepatozoon sp. in African wild dogs (Lycaon pictus) in South Africa
Available online at www.sciencedirect.com Veterinary Parasitology 157 (2008) 123 127 Short communication Molecular detection of Babesia rossi and Hepatozoon sp. in African wild dogs (Lycaon pictus) in
Protocols. Internal transcribed spacer region (ITS) region. Niklaus J. Grünwald, Frank N. Martin, and Meg M. Larsen (2013)
Protocols Internal transcribed spacer region (ITS) region Niklaus J. Grünwald, Frank N. Martin, and Meg M. Larsen (2013) The nuclear ribosomal RNA (rrna) genes (small subunit, large subunit and 5.8S) are
ISTEP+: Biology I End-of-Course Assessment Released Items and Scoring Notes
ISTEP+: Biology I End-of-Course Assessment Released Items and Scoring Notes Page 1 of 22 Introduction Indiana students enrolled in Biology I participated in the ISTEP+: Biology I Graduation Examination
2 Materials and Methods
2 Materials and Methods 2.1 Materials 2.1.1 Chemicals, reagents and consumable materials Chemicals were used from the companies Sigma (München), Roth (Karlsruhe), Merck (Darmstadt), Fluka (Neu-Ulm), Serva
Irina V Nesterova, Cecily A. Bennett, S. Sibel Erdem, Robert P. Hammer, Prescott L. Deininger, and Steven A. Soper
ear-ir Single Fluorophore Quenching System Based on Phthalocyanine (Pc) Aggregation and its Application for Monitoring Inhibitor/Activator Action on a Therapeutic Target: L1-E Irina V esterova, Cecily
http://www.life.umd.edu/grad/mlfsc/ DNA Bracelets
http://www.life.umd.edu/grad/mlfsc/ DNA Bracelets by Louise Brown Jasko John Anthony Campbell Jack Dennis Cassidy Michael Nickelsburg Stephen Prentis Rohm Objectives: 1) Using plastic beads, construct
Introduction to Bioinformatics (Master ChemoInformatique)
Introduction to Bioinformatics (Master ChemoInformatique) Roland Stote Institut de Génétique et de Biologie Moléculaire et Cellulaire Biocomputing Group 03.90.244.730 rstote@igbmc.fr Biological Function
Metabolic Engineering of Escherichia coli for Enhanced Production of Succinic Acid, Based on Genome Comparison and In Silico Gene Knockout Simulation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Dec. 2005, p. 7880 7887 Vol. 71, No. 12 0099-2240/05/$08.00 0 doi:10.1128/aem.71.12.7880 7887.2005 Copyright 2005, American Society for Microbiology. All Rights
HiPer RT-PCR Teaching Kit
HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The
Molecular Typing of Bacillus anthracis using Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA-8)
Defence Research and Development Canada Recherche et développement pour la défense Canada Molecular Typing of Bacillus anthracis using Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA-8) C.W.
pentr Directional TOPO Cloning Kits
user guide pentr Directional TOPO Cloning Kits Five-minute, directional TOPO Cloning of blunt-end PCR products into an entry vector for the Gateway System Catalog numbers K2400-20, K2420-20, K2525-20,
PrimeSTAR HS DNA Polymerase
Cat. # R010A For Research Use PrimeSTAR HS DNA Polymerase Product Manual Table of Contents I. Description...3 II. III. IV. Components...3 Storage...3 Features...3 V. General Composition of PCR Reaction
Chapter 5. Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development
Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development This chapter has been adapted from: W.K. Smits*, C.C. Eschevins*, K.A. Susanna, S. Bron, O.P.
DNA FINGERPRINTING AND PHYLOGENETIC ANALYSIS OF BACTERIA. DNA fingerprinting and the bacterial 16S-23S rrna intergene region.
MCB4403L SUPPLEMENTAL EXERCISE #3: DNA FINGERPRINTING AND PHYLOGENETIC ANALYSIS OF BACTERIA INTRODUCTION DNA fingerprinting and the bacterial 16S-23S rrna intergene region. Relationships among bacteria
PCR & DNA Sequencing. PCR= Polymerase Chain Reaction. PCR applications
PCR= Polymerase Chain Reaction PCR & DNA Sequencing Biology 224 Instructor: Tom Peavy March 20, 2006 DNA photocopier integral tool for molecular biologists work horse versatile (many applications) not
http://hdl.handle.net/10197/2727
Provided by the author(s) and University College Dublin Library in accordance with publisher policies. Please cite the published version when available. Title Performance of DNA data embedding algorithms
Nucleic Acid Chemistry Laboratory Automated DNA Sequencing Manual
Nucleic Acid Chemistry Laboratory Automated DNA Sequencing Manual To DNA Sequencers: This packet contains lots of information that will help you optimize your automated DNA sequencing samples. We have
Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company Chapter 8: Recombinant DNA 2002 by W. H. Freeman and Company
Genetic engineering: humans Gene replacement therapy or gene therapy Many technical and ethical issues implications for gene pool for germ-line gene therapy what traits constitute disease rather than just
Rapid and Simple Amplification of Genomic DNA Sequences Flanking Transposon
MICROBIOLOGY INDONESIA, April 2007, p 1-4 Volume. 1, Number 1 ISSN 1978-3477 Rapid and Simple Amplification of Genomic DNA Sequences Flanking Transposon ARIS TRI WAHYUDI Department of Biology, Institut
costs because of the use of universal fluorogenic reporters. 2012 American Association for Clinical Chemistry
Clinical Chemistry 58:11 1546 1556 (2012) Molecular Diagnostics and Genetics Mediator Probe PCR: A Novel Approach for Detection of Real-Time PCR Based on Label-Free Primary Probes and Standardized Secondary
PicoMaxx High Fidelity PCR System
PicoMaxx High Fidelity PCR System Instruction Manual Catalog #600420 (100 U), #600422 (500 U), and #600424 (1000 U) Revision C Research Use Only. Not for Use in Diagnostic Procedures. 600420-12 LIMITED
Commonly Used STR Markers
Commonly Used STR Markers Repeats Satellites 100 to 1000 bases repeated Minisatellites VNTR variable number tandem repeat 10 to 100 bases repeated Microsatellites STR short tandem repeat 2 to 6 bases repeated
DNA Fingerprinting. Unless they are identical twins, individuals have unique DNA
DNA Fingerprinting Unless they are identical twins, individuals have unique DNA DNA fingerprinting The name used for the unambiguous identifying technique that takes advantage of differences in DNA sequence
PROTOCOL: Illumina Paired-end Whole Exome Capture Library Preparation Using Full-length Index Adaptors and KAPA DNA Polymerase
PROTOCOL: Illumina Paired-end Whole Exome Capture Library Preparation Using Full-length Index Adaptors and KAPA DNA Polymerase This protocol provides instructions for preparing DNA paired-end capture libraries
Bio 102 Practice Problems Recombinant DNA and Biotechnology
Bio 102 Practice Problems Recombinant DNA and Biotechnology Multiple choice: Unless otherwise directed, circle the one best answer: 1. Which of the following DNA sequences could be the recognition site
BD BaculoGold Baculovirus Expression System Innovative Solutions for Proteomics
BD BaculoGold Baculovirus Expression System Innovative Solutions for Proteomics Table of Contents Innovative Solutions for Proteomics...........................................................................