AP BIOLOGY 2006 SCORING GUIDELINES (Form B) Question 2
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1 AP BIOLOGY 2006 SCORING GUIDELINES (Form B) Question 2 The relationship of structure to function is one of the major themes in biology. For three of the following structure/function pairs, describe the structure and then explain how the function is related to the structure. (a) Enzyme structure/catalysis (4 points maximum) Description (2 points) 3-D shape that results from folding of polypeptide chains Folding produces a pocket in which substrate may bind Levels of protein structure (primary, secondary, tertiary) Explanation (2 points) Complementary 3-D shape of enzyme and substrate are required for proper interaction and catalysis in active site reduction of activation energy; induced fit Allosteric modulation, effect of ph, temperature (or other environmental factors) on enzyme shape Elaboration points: competitive/non-competitive inhibition effect on enzyme action; amino acid side groups in active site interact with substrate to stress bonds in substrate and reduce activation energy of reaction (b) mrna structure/protein synthesis (4 points maximum) Description (2 points) Linear sequence of RNA nucleotides Details: 5' cap; poly-a tail; introns Description of origin and/or fate of mrna (transcription, processing and translation) Fine details of RNA nucleotide structure Explanation (2 points) The linear sequence of RNA nucleotides, read as codons (three at a time; contiguous; nonoverlapping) specify the sequence of amino acids incorporated in a new protein being constructed at a ribosome start codon and/or stop codon roles (c) Cell membrane structure/signal transduction (4 points maximum) Description (2 points) A phospholipid bilayer that incorporates malleable (and, often, mobile) integral or membrane associated proteins Membrane-embedded receptor molecules with transmembrane domains Explanation (2 points) Receptor proteins undergo shape changes when proper stimulus is present signal is communicated through membrane by allosteric shape change The altered proteins may then influence other cellular events or states: activation of G-proteins and/or tyrosine-kinase receptor protein auto- and heterophosphorylations leading to cellular response 2006 The College Board. All rights reserved. 3
2 AP BIOLOGY 2006 SCORING GUIDELINES (Form B) Question 2 (continued) (d) Membrane protein structure/active transport or facilitated diffusion (4 points maximum) Description (2 points) Phospholipid bilayer (credited unless already described in c) Integral protein in membrane Protein s 3-D shape allows it to act as a channel, bind solutes, and/or bind ATP, as necessary Explanation (2 points) Some solutes, like ions and larger hydrophobic molecules cannot cross phospholipid membranes unassisted. Integral proteins allow such substances to pass: hydrophilic channel; binding of solute leads to shape change in protein. Hydrolysis of ATP causes shape change in protein leading to shuttle of material from one side of membrane to the other The College Board. All rights reserved. 4
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11 AP BIOLOGY 2006 SCORING COMMENTARY (Form B) Question 2 Sample: 2A Score: 9 In part (a) the maximum of 4 points was earned. A description point was earned for the enzyme holding the substrate in a position that facilitates the reaction, and an explanation point was earned for the 3-D shape of the enzyme being critical to the active site s function. An explanation point was earned for the effect of allosteric inhibitors on the shape of the active site, and a description point for the levels of protein structure. In part (b) the maximum of 4 points was earned. One description point was earned for the mrna being a transcript of a segment of DNA, and another description point for the mrna lacking introns. One explanation point was earned for the codons being nucleotides read three at a time, and another explanation point for the start and stop codons. In part (d) the response earned a description point for transmembrane proteins allowing facilitated diffusion. Sample: 2B Score: 8 In part (a) the response earned 3 points. A description point was earned for the enzyme folding to form a specific bonding site for the substrate, and an explanation point for induced fit. Another explanation point was earned for the effect of ph and temperature. In part (b) the maximum of 4 points was earned. One description point was earned for the mrna being a transcript of a segment of DNA, and another description point for the cap and poly-a tail. One explanation point was earned for the codons being nucleotides read three at a time, and another explanation point for the start and stop codons. In part (c) the response earned a point for the activation of the g-protein. Sample: 2C Score: 6 In part (a) the response earned a description point for a substrate binding to an active site with a certain shape, and an explanation point for the effect of allosteric inhibitors. In part (b) the response earned 1 point for the description of mrna as a sequence of nucleotides, another point for the description of the cap and poly-a tail, and a third point for the explanation of the role of the start codon. In part (c) the response earned a point for the description of the composition of the cell membrane. No points were earned for the mixture of facilitated diffusion and cell signaling The College Board. All rights reserved.
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