Agilent GC Solutions. Advantages of Using Low Thermal Mass Oven (LTM) in Gas Chromatography CAG. Kelly Beard GC Applications October 17, 2012

Transcription

1 Agilent GC Solutions Advantages of Using Low Thermal Mass Oven (LTM) in Gas Chromatography CAG Kelly Beard GC Applications October 17, 2012

2 LTM Series II Key Points Control by 7890 GC and GC, GC/MS SW Platforms Constant Flow Mode - Faster runs - Easier method set-up particularly 2D Easier, Less Risky Column Installation

3 LTM Series II LTM Integrated w/ 7890 GC - Hardware - Firmware - Software

4 Ultimate Union Bracket for Robust Connections

5 Example LTM II GC Driver UI GC oven screen remains the same except for the addition of the LTM tabs The number of tabs will equal the number of LTM s installed, and will automatically appear from the GC driver.

6 Temp (oc) LTM - Low Thermal Mass Technology Fast Temperature Ramps < 400 o C/min Typical <400 o C/min Temperature Range 120 V Oven Fast Ramps Insert ** ( C) ( C/min) > 200 V, 15A > 200 V, 15A 50 to to to to to * * Requires G Fast Cool-Down LTM Cooldown Times (Standard Size) Time (sec) 2m LTM 5m LTM 10m LTM 7890 GC

7 Constant Pressure vs Constant Flow C20 C10 C30 C40 C20 C10 C30 C40

8 Why Use a Low Thermal Mass system?

9 LTM II 1. high throughput (not too complex sample) TPH/HOI analysis contract labs SIMDIST residual solvents 2. Researchers interested in multidimensional GC Deans switch needs LTM (or two ovens) GCxGC with second dimension temperature offset 3. Special configurations In a world of emerging MS technologies, the importance of the (GC) separation should not be underestimated: better separation, backflushing, heart-cutting, etc improves MS data quality, even with QQQ or QTOF instruments

10 Environmental TPH - Total Petroleum Hydrocarbon Need for high through-put, especially for low margin samples like TPH, where analytical cycle times are a critical element in making a profit Key Sales Tools EN Ultra-Fast Total Petroleum Hydrocarbons (TPH) Analysis with Agilent Low Thermal Mass (LTM) GC and Simultaneous Dual-Tower Injection (Wei & Szelewski) - 9x faster TPH throughput using LTM - 18x faster TPH using LTM and dual simultaneous injection

11 Developing Faster GC Run Times for TPH Analysis (C 10 C 44 Standard Shown) n-c10 n-c10 n-c10 n-c12 n-c12 n-c12 n-c14 n-c14 n-c14 n-c16 n-c16 n-c18 n-c16 n-c20 n-c22 n-c23 n-c24 n-c26 n-c28 n-c30 n-c32 n-c18 n-c18 n-c20 n-c20 n-c22 n-c22 n-c23 n-c24 n-c23 n-c24 n-c26 n-c36 n-c26 n-c28 n-c28 n-c30 n-c30 n-c32 n-c32 n-c40 n-c36 n-c36 n-c40 n-c40 n-c44 n-c44 n-c44 pa pa pa o C/min 30m Column o C/min 15m Column o C/min 5m Column LTM/7890 min min Standard GC Run Time 2x Faster, x Faster, LTM/ min

12 LTM Also Greatly Reduces Cool-Down Times, 9x Faster Heating/Cooling Cycle Times GC Run Time Cool Down 40 min min 20 min min 3 min + 2 min 9x Faster Cycle Time LTM/7890

13 Hydrocarbon Processing Industry (HPI) Shorter analytical cycle times get data back to operations faster, or allow more samples/shift (eg. Simulated Distillation) Key App Notes EN EN EN Fast Hydrocarbon & Sulfur Simulated Distillation Using the Agilent Low Thermal Mass (LTM) System on the 7890A GC and 355 Sulfur Chemiluminescence Detector (Wang, Firor, Tripp) Automated Preparation of Simulated Distillation Samples for ASTM Methods D2887, D7213, D7398 and D6352 using a Dual Tower 7693A and Tray System (sample preparation) Boiling Point Range of Fatty Acid Methyl Esters Using the 7890A Gas Chromatograph, Low Thermal Mass (LTM) System, and 7693A Tower and Tray (Biodiesels) - Fast Simdist (< 3 mins), 6x faster than conventional Simdist - Dual channel, simultaneous SimDist for hydrocarb s & sulfur A/Tray system for sample preparation

14 C20 C24 C28 C32 C36 C40 C18 C6 C7 C8 C 9 C11 C15 C17 C5 C14 C16 C10 C12 Fast Simulated Distillation Under 3 Minutes Using LTM 6x faster compared to conventional ASTM D2887 procedure pa ASTM D2887 Reference Oil LTM Module : 45ºC to 350ºC at 150ºC/min Faster than conventional GC ( o C/min max) min pa Calibration sample C5-C44 LTM Module : 45ºC to 350ºC at 150ºC/min min

15 PAH by LTM/GC/MS Fast Analysis - LTM 20 m x 0.18 mm ID x 0.18 µm DB- 5MS Time--> Reference method Standard Oven 30 m x 0.25 mm ID x 0.25 µm DB- 5MS Time-->

16 Customers are using LTM for Mineral Oil Analysis

17 LTM for 2D GC, GC/MS R&D Independent column temperature control Independent temperature control (2, 3 columns in 1 GC) Dual simultaneous injections with independent temp. programs Multidimensional analysis Deans switch heartcutting GCXGC Flow modulator in offset LTM + CFT!!!

18 EN

19 7683 ALS 5975 MSD FID S/Sl R2 R1 HP-5MS Deans Switch DB-17MS LTM 7890 GC

20 Perfume sample Dimension 1 (FID) Abundance Time-->

21 Perfume sample Dimension 1 (FID) Heart-cut Abundance Cut 1 Cut 2 Cut Time-->

22 Abundance Perfume sample Dimension 2 (MSD) 4.2e e e e+07 A 24 +? 1e+07 2e e e+07 2e e+07 1e+07 5e+06 B Time-->

23 EN

24 Abundance Chiral column Fast temperature program Linalool (No Chiral Separation) Stationary Phase Bleed Time-->

25 Abundance Chiral column slow temperature program Separation of Enantiomers Time-->

26 Set-up to FID from column 1 to column 2

27 Test mixture on Dim 1 scouting run Response_ Time A min

28 Test mixture on Dim 1 - heart-cut run Response_ Time Heart-Cut (1+2) min B min

29 Abundance Time--> Abundance 1+2 Dim 2 GC: 50 (1) (20) LTM: =GC C EIC (SCAN) Time--> EIC (SCAN) GC: 50 (1) (20) LTM: 50 (11) (4) (3.1) D

30 Agilent Apps, Key Publications, Gerstel/RIC Apps Environmental EN Mineral Oil/HOI GC #1 Enviro LTM app in EMEA EN TPH GC #1 Enviro LTM app in USA EN PAH GC/MS Show LTM with GC/MS w/ CFT/Backflush Good MSD resolution, low column maintenance an PCB s GC HPI EN SimDis GC Simdis HPI LTM app EN SimDis/MMI/7693 GC Simdis of FAMES/biodiesel, MMI inlet, sample prep EN SimDis/MMI/7693 GC Simdis ASTM 2887 (& more), MMI, sample prep 2D with Dual Independent Column Temperature Control (Unique to LTM/Agilent) EN Pharma, Fragrance GC/MS 2D-CFT/Deans chiral analysis EN Food/Fragrance GC/MS 2D-CFT/Deans allergens J of Sep Science HPI (Dow/Luong) GC 2D-CFT/Deans, HPI examples Toxicology/Forensics an THC in blood/urine GC/MS THC using LTM/GC/MS with CFT/Deans & Backflush (low column maintenance) (Agilent is co-author) Pharma EN Residual solvents GC #1 GC application in Pharma

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