Annex to the Accreditation Certificate D-PL according to ISO/IEC 17025:2005
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1 Deutsche Akkreditierungsstelle GmbH Annex to the Accreditation Certificate D-PL according to ISO/IEC 17025:2005 Period of validity: to Holder of certificate: Eurofins GeneScan GmbH Engesserstraße 4, Freiburg Tests in the fields: biomolecular species identification and analysis for genetically modified organisms and derived products in food, feed, additives, dietary supplements, flavor, vegetable raw materials and seeds Abbreviations used: see last page The laboratory is permitted within the specified testing fields marked with */**, without being required to inform and obtain prior approval from DAkkS: *) the free choice of standard or equivalent test methods. **) the modification, development and refinement of test methods. The listed test methods are exemplary. The laboratory maintains a current list of all test methods in a flexible scope of accreditation. This document is a translation. The definitive version is the original German annex to the accreditation certificate. 1/5
2 1 Biomolecular species identification and analysis for genetically modified organisms and derived products in food, feed, additives, dietary supplements, flavor, vegetable raw materials and seeds using qualitative nucleic acid based testing methods ** DIN EN ISO DIN EN ISO GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P modified organisms and derived products - General requirements and definitions modified organisms and derived products - Qualitative nucleic acid based methods Identification of corn species Modification specific detection of Liberty Link Corn Modification specific detection of BT 176 Corn Modification specific detection of Roundup Ready -Soybean Modification specific detection of YieldGard Corn Modification specific detection of StarLink Corn 2 Biomolecular species identification and analysis for genetically modified organisms and derived products in food, feed, additives, dietary supplements, flavor, vegetable raw materials and seeds using qualitative nucleic acid and fluorescence based testing methods ** GSE-P GSE-P GSE-P GSE-P Detection of bovine DNA Detection of pig DNA Detection of chicken DNA Detection of turkey DNA Period of validity: to Translation - 2/5
3 GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P GSE-P EFGA /02.49 Event specific detection of NK603 Corn Event specific detection of MIR604 Corn Element specific detection of Cauliflower Mosaic Virus 35S promoter Element specific detection of Agrobacterium tumefaciens Nopalin Synthase-terminator Event specific detection of MON863 corn Event specific detection of Herculex Rootworm line DAS Event specific detection of A Soy Event specific detection of Bt63-Rice Identification of rape species Identification of soy species Element specific detection of 35S promoter of Cauliflower Mosaic Virus Identification of tobacco species Evaluation of qualitative PCR Period of validity: to Translation - 3/5
4 3 Biomolecular species identification and analysis for genetically modified organisms and derived products in food, feed, additives, dietary supplements, flavor, vegetable raw materials and seeds using quantitative nucleic acid and fluorescence based testing methods ** DIN EN ISO GSE-P GSE-P GSE-P GSE-P EFGA /02.39 EFGE 407 L02e modified organisms and derived products - Quantitative nucleic acid based methods Soy event A Soy (event specific) RR- Corn type GA21 (event specific) Roundup Ready-Rape RT73 (event spezific) Quantification of 35S-Promoter in Cotton Evaluation of quantitative PCR Detectionsystems 4 Biomolecular species identification and analysis for genetically modified organisms and derived products in food, feed, additives and seeds using polymerase chain reaction (PCR) * ASU L ASU L Schweizerisches Lebensmittelbuch Kap. 52B Detection of a genetic modification of soybeans Screening for the detection of genetically modified DNA sequences in plant Swiss screening for the detection of genetically modified DNA sequences in plants Period of validity: to Translation - 4/5
5 5 Further applicable documents: DNA extraction methods from food, feed, additives, dietary supplements, flavor, vegetable raw materials and seeds ** DIN EN ISO EFGA / EFGA /02.04 EFGA / EFGA / EFGA / EFGA /02.10 EFGA /02.13 EFGA /02.46 EFGA /02.52 EFGE 407 L01e modified organisms and derived products - Nucleic acid extraction DNA-extraktion CTAB/Qiagen DNA-extraktion CTAB/Chloroform/Isopropanol DNA-extraktion from tobacco - Modified Swiss Screening DNA-extraktion from hexane soluble samples DNA-extraktion from samples with high salt content DNA-extraktion from chloroform soluble samples DNA-extraktion from cotton DNA-extraktion from honey Standard Addition: DNA extraktion CTAB-MN-KF_K4 Common Types of Samples - Sample Preparation and DNA Extraction Abbreviations used: ASU DIN EFGA EFGE EN GSE IEC ISO Official collection of 64 LFGB German Institute for Standardization Eurofins GeneScan (internal testing method) Eurofins GeneScan (additional QMH) European Standard GeneScan Europe AG (internal testing method) International Electrotechnical Commission International Organization for Standardization Period of validity: to Translation - 5/5
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