Kits Product List V4

Size: px
Start display at page:

Download "Kits Product List 2016 - V4"

Transcription

1 Kits Product List V4

2 Content Watch out for our products! DNA Extraction 3 GMO and Plant and Plant Pathogen Species RT-PCR GMOScreen Kits (RT-PCR) 4 GMOIdent Kits (RT-PCR) 6 SpeciesScreen and SpeciesIdent Kits (RT-PCR) 9 GMO and Plant Species Conventional PCR SpeciesIdent and GMOScreen Kits (gel-based) 10 GMOIdent Kits (gel-based) 11 GMOQuant RT-PCR Verification Kits for GMOQuant 12 GMOQuant Kits (low ROX ) 13 GMOQuant Kits (high ROX ) 15 Control DNA GMO and Plant Control DNA GMOs 17 Control DNA Plant Species 19 DNAnimal RT-PCR and Control DNA Animal DNAnimal Kits 20 Meat Standards and Control DNA Animal Species 22 Food Pathogen Kits BACGene real-time PCR Kits 24 BIOLINE ELISA Kits 24 Ordering and Shipping Information 24 General Terms and Conditions 25 Eurofins GeneScan real-time PCR kits: FAQ 29 Terms and Conditions Products are sold by Eurofins GeneScan GmbH, a member of the Eurofins Group. Kits are subject to changes with regard to content and price and are intended for research use only. All sales are subject to the attached General Terms and Conditions of Eurofins GeneScan GmbH. Valid from March 29, Terms and Conditions and prices of previous price lists expire. Delivery on buyer s expense and risk, EXW Freiburg Incoterms 2000, prepaid and added, unless agreed differently between the parties. Refunds and Credits are not provided on unused or expired kits. Real-time PCR kits: The Taqman process is covered by patents and patent applications issued to Hoffmann-La Roche AG and applicable in certain countries. Eurofins GeneScan does not encourage or support the unauthorised or unlicensed use of these processes. Use of these products is recommended for persons that either have a license to perform PCR or are not required to obtain a license. SeedLink, LibertyLink, StarLink, Maximizer, RoundupReady, YieldGard, Herculex : These are trademarks of Bayer CropScience, Syngenta, Monsanto and Dow AgroScience. All trademarks mentioned in this document, even if not marked as such, are not to be considered unprotected. 2/30 Kits Product List, 2016 V4

3 DNA Extraction Cat # Item Description DNA Extraction and Electrophoresis Different DNA extraction kits serve for different sample types: The DNAExtractor is buffer-based and especially recommended for raw materials, soy products and grains/seed in general. Furthermore, there is the DNAExtractor Fat kit for DNA extraction from emulsifiers like lecithin, oils and fats. The GENESpin kit is very convenient to use for all kinds of food, feed samples. For real-time PCR applications, especially for grain and processed food samples, we recommend an additional DNA purification with our DNA Cleaning Columns following the DNA extraction GENESpin DNA extraction kit for 50 food or feed samples x GENESpin 10 DNA extraction kits for 50 food or feed samples each x GENESpin 30 DNA extraction kits for 50 food or feed samples each x GENESpin 50 DNA extraction kits for 50 food or feed samples each Additional lysis buffer for GENESpin 1000 ml buffer CF Additional buffer C4 for GENESpin DNA Cleaning Columns 250 ml C4 buffer 100 DNA cleaning columns x DNA Cleaning Columns 10 x DNA Cleaning Columns 5 x 100 DNA cleaning columns 10 x 100 DNA cleaning columns DNAExtractor DNA extraction kit for 50 seed, grain, food or feed samples (solvents not included) Additional lysis buffer for DNAExtractor DNAExtractor Fat for lecithin, oil and fat Special lysis buffer for lecithin, oil and fat for use with DNAExtractor Fat DNAExtractor Honey (Qiagen/Retsch Tissue Lyser needed) DNA Length Standard puc18 HAIII size marker bp 500 ml DNA extraction kit for 50 oil, fat or lecithin samples, solvents not incl. 2 x 50 ml DNA extraction kit for 50 honey/pollen samples 500 µl, 0.05 µg/µl RNase 5 mg/ml, 0.5 ml Proteinase K 20 mg/ml, 750 µl 3/30

4 GMOs and Plant Species: Qualitative Real-time PCR Cat # Item Description GMOScreen kits for Real-time PCR Our most convenient and economic approach to GMO screening: Duplex or Multiplex PCR for comprehensive GMO screenings. The kits contain all reagents and controls needed. Please check the required filter sets for your RT-PCR machine. Excel evaluation sheets are available on request GMOScreen RT IPC (LR) 35S/NOS/FMV, for ABI 7500 Fast x GMOScreen RT IPC (LR) 35S/NOS/FMV GMOScreen RT IPC (NR) 35S/NOS/FMV for MX3005P, AriaMX, CFX96 and LC x GMOScreen RT IPC (NR) 35S/NOS/FMV GMOScreen RT IPC (LR) 35S/NOS GMOScreen RT 35S/IPC and NOS/IPC (LR/HR+) GMOScreen RT (LR/HR+) 35S/IPC and NOS/IPC and FMV/IPC GMOScreen RT FMV/IPC (LR/HR+) GMOScreen RT IPC (LR) SAMS-ALS GMOScreen RT IPC (LR) CV127/SAMS-ALS GMOScreen RT IPC (LR) AgroBorder I/II GMOScreen RT IPC (LR) GM Soy I GMOScreen RT IPC (NR) GM Soy I GMOScreen RT IPC (LR) bar/pat 96 RT-PCR reactions, up to 44 samples per plate. Multiplex screening of pfmv, p35s and tnos in a single tube with internal positive control (IPC). Validated for use on ABI7500/Fast. 10 x for ABI7500/Fast 96 RT-PCR reactions, up to 44 samples per plate. Multiplex screening of pfmv, p35s and tnos with internal positive control. Validated for use on MX3005P, AriaMX and CFX96 and LC x for MX3005P, AriaMX, CFX96 and LC RT-PCR reactions, up to 44 samples per plateduplex PCR with internal positive control. Validated for ABI7500/Fast, MX3005P and LC x 96 RT-PCR reactions, for up to 46 samples. For use on low-rox and high-rox cyclers with FAM and VIC filters. 3 x 96 RT-PCR reactions, for up to 46 samples. For use on low-rox and high-rox cyclers with FAM and VIC filters. 96 RT-PCR reactions, for up to 46 samples. For low- and high- ROX cyclers with FAM/VIC filters. 96 RT-PCR reactions, for up to 44 samples. Detection of SAMS-als modification (e.g. DP356043/ DP soy) with IPC for inhibition control. 96 RT-PCR reactions, for up to 44 samples. Duplex-detection of CV127 soy and SAMS-als modification (e.g. DP356043/ DP soy) with IPC for inhibition control. Validated for ABI7500/Fast, MX3005P and LC RT-PCR reactions, for up to 44 samples. Duplex-detection of AgroBorder I and II with IPC for inhibition control. Validated for ABI7500/Fast, MX3005P, AriaMX and LC480. GMOScreen for comprehensive GMO detection in Soy (LR) for ABI 7500 Fast GMOScreen for comprehensive GMO detection in Soy (NR) for MX3005P and LC RT-PCR reactions, for up to 44 samples. Duplex-screening with internal positive control (IPC) for inhibition control. 4/30 Kits Product List, 2016 V4

5 Cat # Item Description GMOScreen Kits for Real-time PCR GMOScreen RT IPC (LR) Event GA21/PMI-NOS GMOScreen RT IPC (LR) CsVMV GMOScreen RT (UMM) Cry1Ab/Ac 96 RT-PCR reactions, for up to 44 samples. Duplex-screening for GA21 and pmi/nos mod. (MIR604/MIR162/3272/5307) with IPC for inhibition control. Only for use on MX3005P/MX3000P! 96 multiplex-rt-pcr rxns, for up to 44 samples. Screening for i.e. DAS , DAS , DAS soy.validated for ABI7500/Fast, MX3005P/ MX3000P, AriaMX, CFX96 and LC RT-PCR reactions, up to 44 samples per plate. Accessory Kits for Real-time PCR Verification Kit for GMOScreen RT (35S/NOS/FMV) Eurofins Color Compensation Kit FAM, HEX Eurofins Color Compensation Kit FAM, R6G Eurofins Color Compensation Kit FAM, HEX, Cy5 Eurofins Color Compensation Kit FAM, JOE, Cy5 Eurofins Color Compensation Kit FAM, Cy5, R6G Eurofins Color Compensation Kit FAM, HEX, Cal610, Cy5 Verification kit for GMOScreen RT kits (35S/NOS and 35S/NOS/FMV (IPC)); LOD verification, DNAs, instruction and evaluation table included. One GMOScreen RT kit needed additionally. Eurofins Color Compensation Kit for kits with FAM, HEX labelling (i.e. for GMOScreen RT IPC FMV/IPC) Eurofins Color Compensation Kit for kits with FAM, R6G labelling (i.e. for GMOScreen RT IPC CsVMV) Eurofins Color Compensation Kit for kits with FAM, HEX, Cy5 labelling (i.e. for DNAnimal Screen Halal IPC) Eurofins Color Compensation Kit for kits with FAM, JOE, Cy5 labelling (i.e. for GMOScreen RT IPC bar/pat) Eurofins Color Compensation Kit for kits with FAM, Cy5, R6G labelling (i.e. for GMOScreen RT IPC AgroBorder I/II) Eurofins Color Compensation Kit for kits with FAM, HEX, Cal610, Cy5 labelling (i.e. for GMOScreen RT IPC 35S/NOS/FMV) 5/30

6 Cat # Item Description GMOIdent RT Kits (Qualitative Real-time PCR, LR + HR) The GMOIdent RT product line enables you to identify and/or exclude single GMO varieties in your sample with real-time PCR on many RT cyclers. Many kits contain an internal positive control (IPC). Most kits employ the GMO PCR systems from the official European EURL methods. The kits are validated for use on ABI 7900, ABI7500/Fast and Agilent MX3005P and can beyond this be used on most RT-PCR instruments (LR and HR), if not mentioned otherwise. Corn GMOIdent RT Bt176 Corn GMOIdent RT Event Bt11 Corn GMOIdent RT Event MIR162 Corn GMOIdent RT (IPC) Event T25 Corn GMOIdent RT (IPC) Event NK603 Corn GMOIdent RT (IPC) Event MIR604 Corn GMOIdent RT (IPC) Event DAS Corn GMOIdent RT (IPC) Event TC1507 Corn GMOIdent RT (IPC) Event MON810 Corn GMOIdent RT (IPC) Event MON89034 Corn GMOIdent RT (IPC) Event MON863 Corn GMOIdent RT (IPC) Event MON88017 Corn GMOIdent RT (IPC) Event GA21 Corn GMOIdent RT (IPC) Event 3272 Corn GMOIdent RT (IPC) Ly038 Corn GMOIdent RT (IPC) Event MON87460 Corn GMOIdent RT (IPC) Event DAS Corn Kit with 96 real-time PCR rxns for modification-specific detection of Bt176 corn Kit with 96 real-time PCR rxns for event-specific detection of Bt11 corn Kit with 96 real-time PCR rxns for event-specific detection of MIR162 corn Kit with 96 real-time PCR rxns for event-specific detection of T25 corn with internal pos. control Kit with 96 real-time PCR rxns for event-specific detection of NK603 corn with internal pos.control Kit with 96 real-time PCR rxns for event-specific detection of MIR604 corn with intern. pos. control Kit with 96 real-time PCR rxns for event-specific detection of Herculex RW DAS corn with internal positive control Kit with 96 real-time PCR rxns for event-specific detection of Herculex I TC1507 corn with internal positive control Kit with 96 real-time PCR rxns for event-specific detection of MON810 corn with intern. pos. contr. Kit with 96 real-time PCR rxns for event-specific detection of MON89034 corn with internal positive control Kit with 96 real-time PCR rxns for event-specific detection of MON863 corn with internal positive control Kit with 96 real-time PCR rxns for event-specific detection of MON88017 corn with internal positive control Kit with 96 real-time PCR rxns for event-specific detection of GA21 corn with internal pos. control Kit with 96 real-time PCR rxns for event-specific detection of 3272 corn with internal pos. control Kit with 96 real-time PCR rxns for modification-specific detection of Ly038 corn with internal positive control Kit with 96 real-time PCR rxns for event-specific detection of MON corn with internal positive control Kit with 96 real-time PCR rxns for event-specific detection of Event DAS corn with internal positive control 6/30 Kits Product List, 2016 V4

7 Cat # Item Description GMOIdent RT Kits (Qualitative Real-time PCR, LR + HR) Cotton GMOIdent RT Event MON1445 Cotton GMOIdent RT Event MON88913 Cotton GMOIdent RT Event MON531 Cotton GMOIdent RT Event MON15985 Cotton GMOIdent RT Event LLCotton25 Cotton GMOIdent RT Event Cotton GMOIdent RT Event Cotton GMOIdent RT Event GHB614 Cotton Flax GMOIdent RT IPC (LR/HR+) VIP3Aa Cotton GMOIdent RT (IPC) FP967 Flax Potato GMOIdent RT Event EH Potato Rapeseed GMOIdent RT (IPC) Event T45 Canola GMOIdent RT Event MS8 Rapeseed GMOIdent RT Event Rf3 Rapeseed GMOIdent RT Event RT73 Rapeseed Kit with 96 real-time PCR rxns for event-specific det. of RoundupReady (MON1445) cotton Kit with 96 real-time PCR rxns for event-specific detection of RR Flex (MON88913) cotton Kit with 96 real-time PCR rxns for event-specific det. of Bollgard (MON531/MON15985) cotton Kit with 96 real-time PCR rxns for event-specific det. of Bollgard2 (MON15985) cotton Kit with 96 real-time PCR rxns for event-specific det. of LLCotton25 (ACS-GHØØ1-3) cotton Kit with 96 real-time PCR rxns for event-specific detection of cotton Kit with 96 real-time PCR rxns for event-specific detection of cotton Kit with 96 real-time PCR rxns for event-specific detection of GHB614 cotton Kit with 96 real-time PCR rxns, for detection of Vip3a cotton with internal pos. control Kit with 96 real-time PCR rxns for modification-specific detection of CDC Triffid flax/linseed with internal pos. control Kit with 96 real-time PCR rxns for event-specific detection of Amflora EH potato Kit with 96 real-time PCR rxns for event-specific detection of LibertyLink T45 rapeseed with IPC Kit with 96 real-time PCR rxns for event-specific detection of MS8 rapeseed Kit for event-specific detection of Rf3 rapeseed Kit with 96 real-time PCR rxns for event-specific detection of RoundupReady RT73 rapeseed 7/30

8 Cat # Item Description GMOIdent RT Kits (Qualitative Real-time PCR, LR + HR) Rice GMOIdent RT Event LL62 Rice GMOIdent RT Event LL601 Rice GMOIdent RT LibertyLink Rice GMOIdent RT LibertyLink Rice (with species control) GMOIdent RT Bt Rice GMOIdent RT Bt Rice (with species control) Soy GMOIdent RT (LR) IPC Event A Soy GMOIdent RT (IPC) Event MON89788 Soy GMOIdent RT (IPC) RoundupReady Soy GMOIdent RT (IPC) Event DP Soy GMOIdent RT (IPC) CV127 Soy GMOIdent RT Event A Soy GMOIdent RT Event MON87701Soy GMOIdent RT Event MON87705Soy GMOIdent RT Event FG72Soy Sugarbeet GMOIdent RT (IPC) Event H7-1 Sugarbeet Kit with 96 real-time PCR rxns for event-specific detection of LL62 rice Kit with 96 real-time PCR rxns for event-specific detection of LL601 rice Kit with 96 real-time PCR rxns for detection (96 rxns) of LibertyLink varieties (p35s/bar) in rice Kit plus rice species control (96 rxns each for GMO and rice species) Kit with 96 real-time PCR rxns for detection of Bt63 rice in rice samples (method by Mäde et al.) plus rice species control (96 rxns each for GMO and rice species) Kit with 96 RT-PCR rxns for event-specific det. of LibertyLink A soy with IPC Kit with 96 RT-PCR rxns for event-specific det. of MON89788 soy with intern. pos. contr. Kit with 96 RT-PCR rxns for modification-specific detection of MON soy with IPC Kit with 96 RT-PCR rxns for event-specific detection of DP soy with int. pos. contr. Kit with 96 RT-PCR rxns for event-specific detection of CV127 soy with internal pos. control Kit with 96 RT-PCR rxns for event-specific detection of LibertyLink A soy Kit with 96 RT-PCR rxns for event-specific detection of MON87701 soy Kit with 96 RT-PCR rxns for event-specific detection of MON87705 soy Kit with 96 RT-PCR rxns for event-specific detection of FG72 soy Kit with 96 RT-PCR rxns for event-specific detection of H7-1 sugarbeet with int. pos. control 8/30 Kits Product List, 2016 V4

9 Cat # Item Description SpeciesScreen RT Kits (Qual. Real-time PCR, LR + HR) SpeciesScreen Plant RT IPC Kit with 96 RT-PCR rxns for general plant DNA detection with internal positive control. All reagents and controls included x SpeciesScreen Plant RT IPC 10 Kits with 96 RT-PCR rxns eacn for general plant DNA detection with internal positive control. All reagents and controls included. SpeciesIdent RT Kits (Qualitative Real-time PCR) SpeciesIdent RT Corn/Maize Kit with 96 RT-PCR rxns for corn/maize species detection. All reagents and controls included SpeciesIdent RT Soy IPC (LR/HR) Kit with 96 RT-PCR rxns for soy species detection with internal positive control (IPC). All reagents and controls included SpeciesIdent RT Cotton IPC Kit with 96 RT-PCR rxns for cotton species detection with internal positive control (IPC). All reagents and controls included SpeciesIdent RT Rice IPC Kit with 96 RT-PCR rxns for rice species detection with internal positive control (IPC). All reagents and controls included SpeciesIdent RT CaMV IPC Kit with 96 RT-PCR rxns for Cauliflower Mosaic Virus species detection with internal positive control (IPC). All reagents and controls included SpeciesIdent RT Agrobacterium tumefaciens IPC (LR) Kit with 96 RT-PCR rxns for Agrobacterium tumefaciens species detection with internal positive control (IPC). All reagents and controls included. 9/30

10 GMOs and Plant Species: Conventional PCR Cat # Item Description SpeciesIdent Kits for Gel-based PCR With the SpeciesIdent product line, plant species as potential GMO sources and natural occurance of the virus CaMV (source of the 35s promoter) as well as A. tumefaciens (source of nos promoter and terminator) can be detected/excluded. The kits contain all reagents and controls for conventional PCR SpeciesIdent Cotton Kit for cotton species DNA detection. Premaster and control DNA for 100 rxns SpeciesIdent Corn/Maize Kit for corn/maize species DNA detection. Premaster and control DNA for 100 rxns SpeciesIdent Soy Kit for soy species DNA detection. Premaster and control DNA for 100 rxns SpeciesIdent CaMV Kit for Cauliflower Mosaic Virus reverse transcriptase gene detection. Premaster and control DNA for 100 rxns SpeciesIdent Agrobacterium tumefaciens Kit for Agrobacterium tumefaciens nos gene detection. Premaster and control DNA for 100 rxns GMOScreen Kits for Gel-based PCR The GMOScreen product line is designed for a basic approach to GMO testing: it offers several combinations of screening elements. All kits contain all reagents and controls needed GMOScreen 35S/NOS/FMV Kit for a general screening for GMO in food/feed: detection of the CaMV 35s promoter, the A. tumefaciens nos terminator, the FMV 34s promoter and plant chloroplast DNA as control. Premasters for 100 rxns each, control DNA and gel length marker incl GMOScreen 35S/NOS Kit for a general screening for GMO in DNA from food/feed: detection of CaMV 35s promoter and nos terminator and plant chloroplast DNA. Premaster and control DNA for 100 rxns each, gel length marker incl GMOScreen S 35S/NOS like , but with 50 rxns each GMOScreen Rapeseed Kit for a comprehensive GMO screening in rapeseed samples: detection of nos terminator, pat(syn) gene, Laurical and Roundup Ready GT200 rapeseed with Brassica control reaction. Premasters for 50 rxns each, control DNAs and gel length marker included GMOScreen Rapeseed II Kit for a comprehensive GMO screening in rapeseed samples: detection of nos terminator, pat(syn) gene, PVGT and Laurical rapeseed with Brassica control reaction. Premasters for 50 rxns each, control DNAs and gel length marker included GMOScreen Cry1Ab/Ac Mini-Kit Kit for a general screening for GMO in DNA from food/feed: detection of cry1ab/1ac gene. Premaster and control DNA for 100 rxns. 10/30 Kits Product List, 2016 V4

11 Cat # Item Description GMOIdent Kits (Gel-based PCR Kits) The GMOIdent product line enables you to identify and/or exclude single GMOs in your sample. The GMOIdent kits are designed for conventional PCR and contain control DNA and premasters for GMO and species testing, and a length standard for gel electrophoresis GMOIdent RoundupReady Soy GMOIdent Combi Corn GMOIdent Corn Comprehensive GMOIdent GM Corn GMOIdent Maximizer Bt176 Corn GMOIdent Bt11/Bt10 Corn GMOIdent Event Bt11 Corn GMOIdent LibertyLink TM and T25 Corn GMOIdent Event MON810 Corn GMOIdent MON88017/MON89034 Corn GMOIdent Event NK603 Corn GMOIdent Event MON GMOIdent RoundupReady TM GA21 Corn GMOIdent Event TC1507 Corn GMOIdent Event DAS Corn GMOIdent StarLink Corn GMOIdent Sunup Papaya GMOIdent Mini-Kit Bt63 Rice GMOIdent Mini-Kit FP967 Flax GMOIdent Mini-Kit SeedLink Canola Kit for modification-specific detection of Roundup Ready soy and soy DNA (le1) control, 100 rxns each Kit for detection of Bt11/Bt10, Bt176, MON810 and LibertyLink / T25 corn, with corn (hmga) control PCR, 100 rxns each Kit for detection of Bt11/Bt10, event Bt11, Bt176, MON810, LL, T25, MON863, GA21, NK603, TC1507 and DAS corn, 50 reactions each and 100 rxns corn (hmga) control PCR Kit for detection of Bt11, Bt176, MON810, LL, T25, MON863, GA21, NK603, TC1507, DAS , MON88017/MON89034, MIR162/MIR604/3272, 50 rxns each and 100 rxns corn (hmga) control PCR Kit for modification-specific detection of Bt176 corn with corn DNA (hmga) control PCR, 100 rxns each Kit for modification-specific detection of Bt11 and Bt10 corn with corn (hmga) control PCR, 100 rxns each Kit for event-specific detection of Bt11 corn with corn DNA (hmga) control PCR, 100 rxns each Kit for detection of Liberty-Link TM corn and specific det. of T25 corn with corn control PCR, 100 rxns each Kit for event-specific detection of MON810 corn with corn DNA (hmga) control PCR, 100 rxns each Kit for modi.-specific detection of MON88017 and MON89034 corn with corn contr. PCR, 100 rxns each Kit for event-specific detection of NK603 corn with corn control PCR, 100 rxns each Kit for event-specific detection of MON863 corn with corn control, 100 rxns each Kit for modification-specific detection of GA21 corn with corn DNA (hmga) control PCR, 100 rxns each Kit for event-specific detection of Herculex I TC 1507 corn with corn (hmga) control PCR, 100 rxns each Kit for event-specific detection of Herculex Rootworm DAS corn with control (hmga) control PCR, 100 rxns each Kit for specific detection of StarLink CBH351 corn with corn DNA (hmga) control PCR, 100 rxns each Kit for mod.-specific detection of Sunup /Rain-bow papaya with papaya control PCR, 100 rxns each Kit for mod.-specific detection of Bt63 rice (100 rxns cry1ac/nos) in rice samples (w/o species control!) Kit for mod.-specific detection of CDC Triffid flax (100 rxns) (w/o species control!) Kit for mod.-specific detection of SeedLink rapeseed (100 rxns) (w/o species control!) 11/30

12 GMOs: Quantitative Real-time PCR Cat # Item Description GMOQuant Kits The GMOQuant product line is designed for quantitation of GMOs in your sample. There are specific kits and kits for screening quantitation (35S promoter) available. The kits are validated on ABI, Agilent and Roche instruments, but can be used on any realtime PCR cycler equipped with detection devices for the dyes contained in the kits. All kits contain FAM (reporter dye), ROX (normaliser) and a quencher. The kits contain ready-to-use mastermixes for species and GMO for 48 reactions each, calibration DNA standards and a quantification control DNA. With the setup recommended, the kits can be used for one 96-well plate with 9 samples. GMOQuant Verification Kits Verification kits are available for all GMOQuant kits. To order the corresponding verification kit for a specific GMOQuant kit, please kindly quote the cat. no. of the GMOQuant kit and ask for the corresponding verification kit. The kits come with detailed instructions and an evaluation table for the verification results Verification kit for GMOQuant Screen 35S/Soy and RoundupReady Soy Verification kit for GMOQuant NK603 Corn and GMOQuant Screen 35S/Corn kits Verification kit for GMOQuant MON863 Corn kits Verification kit for GMOQuant RoundupReady Soy and GMOQuant Screen 35S/Soy kits (two corresponding GMOQuant kits needed additionally) Verification kit for GMOQuant NK603 Corn and GMOQuant Screen 35S/Corn kits (two corresponding GMOQuant kits needed additionally) Verification kit for GMOQuant MON863 Corn kits (two corresponding GMOQuant kits needed additionally) Verification kit for GMOQuant MON810 Corn kits Verification kit for GMOQuant GA21 Corn kits Verification kit for GMOQuant kit of choice Verification kit for GMOQuant MON810 Corn kits (two corresponding GMOQuant kits needed additionally) Verification kit for GMOQuant GA21 Corn kits (two corresponding GMOQuant kits needed additionally) Verification kit for GMOQuant kit of choice (two corresponding GMOQuant kits needed additionally) 12/30 Kits Product List, 2016 V4

13 Cat # Item Description (Low ROX, e.g. for ABI7500/Fast, Bio-Rad IQ5, Roche LC 480, Agilent MX3005P, MX3000P) Corn S Screen Corn Event NK603 Corn Event GA21 Corn Event MIR604 Corn Event Bt11 Corn Event MON88017 Corn Event MON89034 Corn Event 3272 Corn Event MON810 Corn Event MON863 Corn Event TC1507 Corn Event DAS Corn Event MIR162 Corn Kit for a GMO screening quantitation with CaMV 35S promoter and corn DNA (hmga) reference PCR, 48 rxns each Kit for event-specific quantitation of NK603 corn with corn DNA (hmga) reference PCR, 48 rxns each Kit for event-specific quantitation of GA21 corn with corn DNA (hmga) reference PCR, 150 rxns each Kit for event-specific quantitation of MIR604 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantification of Bt11 corn with corn (hmga) reference PCR, 48 rxns each Kit for event-specific quantitation of MON88017 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON89034 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of 3272 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON810 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON863 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of Herculex I TC1507 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of DAS corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MIR162 corn with corn DNA (hmga) ref. PCR, 48 rxns each Event Bt176 Corn Kit for event-specific quantitation of Bt176 corn DNA (hmga) ref. PCR, 48 rxns each with corn CBH351 Corn LibertyLink Corn Kit for modification-specific quantitation of StarLink /CBH351 corn with corn DNA reference PCR, 48 rxns each LibertyLink Corn Kit. 48 rxns GMO and species reference each, calibration DNA and reference standard 13/30

14 Cat # Item Description Cotton Cotton Kit for event-specific quantitation of cotton with cotton DNA ref. PCR, 48 rxns each Rapeseed Cotton Event T45 Rapeseed Kit for event-specific quantitation of cotton with cotton DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of T45 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Event RT73 Rapeseed Kit for event-specific quantitation of RT73 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Rice Soy Event MS8 Rapeseed Event Rf3 Rapeseed Event LL62 Rice GMOQuant Duplex (LR) 35S/MON89788 Soy 35S Screen Soy AgroBorder II Screen Soy RoundupReady Soy Event A Soy Event MON89788 Soy Event DP Soy Event DP Soy Event MON87701 Soy Event A Soy Event CV127 Soy Event MON87705 Soy Kit for event-specific quantitation of MS8 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of Rf3 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of LL62 rice with rice DNA ref. PCR, 48 rxns each Kit for a duplex GMO quantitation of CaMV 35S promoter and event MON89788 soy (48 rxns) with soy DNA (le1) reference PCR (48 rxns) Kit for a GMO screening quantitation with soy DNA (le1) ref. PCR, 48 rxns each Kit for screening quantification of different GMOs with soy DNA (le1) ref. PCR, 48 rxns each Kit for modification-specific quantitation of GTS soy with soy DNA (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of A soy with soy DNA (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON89788 soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of DP soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of DP soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON87701 soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of A soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of CV127 soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON87705 soy with soy (le1) ref. PCR, 48 rxns each * Kits marked UMM can be used for both, LR and HR cyclers! 14/30 Kits Product List, 2016 V4

15 Cat # Item Description Corn (High ROX, e.g. for ABI 7300, 7900) 35S Screen Corn Kit for a GMO screening quantitation with corn DNA (hmga) ref. PCR, 48 rxns each Cotton Potato Event Bt176 Corn Event Bt11 Corn Event MON863 Corn Event MON810 Corn LibertyLink TM Corn Event NK603 Corn Event GA21 Corn StarLink Corn Event TC1507 Corn Event DAS Corn Event MIR604 Corn Event MON88017 Corn Event MON89034 Corn Cotton Cotton Event EH Potato Kit for event-specific quantitation of Bt176 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of Bt11 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON863 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON810 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for quantitation of LibertyLink TM (T14/T25) corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of NK603 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of GA21 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for specific quantitation of StarLink TM corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of Herculex I TC1507 corn with corn DNA (hmga) reference PCR, 48 rxns each Kit for event-specific quantitation of Herculex RW DAS corn with corn (hmga) reference PCR, 48 rxns each Kit for event-specific quantitation of MIR604 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON88017 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON89034 corn with corn DNA (hmga) ref. PCR, 48 rxns each Kit for event-specific quantitation of cotton with cotton DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of cotton with cotton DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of EH (Amflora ) potato with UDP-glycose pyrophosphorylase species ref. PCR, 48 rxns each Method verification made easy: Find our new GMOQuant Verification kits on p. 12! 15/30

16 Cat # Item Description (High ROX, e.g. for ABI 7300, 7900) Rapeseed Rice Soy Event T45 Rapeseed Event RT73 Rapeseed Event MS8 Rapeseed Event Rf3 Rapeseed Event LL62 Rice 35S Screen Soy Agroborder II Screen Soy RoundupReady Soy Event A Soy Event MON89788 Soy Event DP Soy Event MON87701 Soy Event A Soy Event CV127 Soy Event MON87705 Soy Kit for event-specific quantitation of T45 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of RT73 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of MS8 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of Rf3 rapeseed with rapeseed DNA ref. PCR, 48 rxns each Kit for event-specific quantitation of LL62 rice with rice DNA (sps) ref. PCR, 48 rxns each Kit for a GMO CaMV p35s screening quantitation with soy DNA (le1) ref. PCR, 48 rxns each Kit for screening quantification of different GMO with soy DNA (le1) ref. PCR, 48 rxns each Kit for modification-specific quantitation of GTS soy with soy DNA (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of A soy with soy DNA (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON89788 soy with soy DNA (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of DP soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON87701 soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of A soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of CV127 soy with soy (le1) ref. PCR, 48 rxns each Kit for event-specific quantitation of MON87705 soy with soy (le1) ref. PCR, 48 rxns each Sugarbeet Event H7-1 Sugarbeet Kit for event-specific quantitation of H7-1 sugarbeet with glutamine synthetase sugarbeet ref. PCR, 48 rxns each * Kits marked UMM can be used for both, LR and HR cyclers! 16/30 Kits Product List, 2016 V4

17 GMOs and Plant Species: Control DNA Cat # Item Packaging Control DNA GMOs Control DNA i.e. for use with GMOIdent kit, if not mentioned otherwise made from 100% (w/w) GMO material. Sorted by species and concentration Genomic DNA 3272 corn, 10% GMO, made from CRM BF420c (SYN-E3272-5) (100 copies GMO/µL) 0.15 ml Genomic DNA Bt11 corn (SYN-BTØ11-1) (100 copies/µl) 0.15 ml Genomic DNA Bt176 corn (SYN-EV176-9) (100 copies/µl) 0.15 ml Genomic DNA CBH351 corn (ACS-ZMØØ4-3) (100 copies/µl) 0.15 ml Genomic DNA GA21 corn (MON-ØØØ21-9) (100 copies/µl) 0.15 ml Genomic DNA Herculex I TC 1507 corn, 10% GMO, from ERM BF418d (DAS-Ø15Ø7-1) Genomic DNA Herculex RW DAS corn, 10% GMO, made from ERM BF424b (DAS ) (100 copies GMO/µL) 0.15 ml (100 copies GMO/µL) 0.15 ml Genomic DNA MIR162 corn (SYN-IR162-4) (100 copies/µl) 0.15 ml Genomic DNA MIR604 corn DNA, 10% GMO, made from ERM BF-423d (SYN-IR6Ø4-5) (100 copies GMO/µL) 0.15 ml Genomic DNA MON810 corn (MON-ØØ81Ø-6) (100 copies/µl) 0.15 ml Genomic DNA MON863 corn (MON-ØØ863-5) (100 copies/µl) 0.15 ml Genomic DNA MON87460 corn (MON-8746Ø-4) (100 copies/µl) 0.15 ml Genomic DNA MON88017 corn (MON-88Ø17-3) (100 copies/µl) 0.15 ml Genomic DNA MON89034 corn (MON-89Ø34-3) (100 copies/µl) 0.15 ml Genomic DNA NK603 corn (MON-ØØ6Ø3-6) (100 copies/µl) 0.15 ml Genomic DNA T25 corn (ACS-ZMØØ3-2) (100 copies/µl) 0.15 ml Genomic DNA DAS (DAS-4Ø278-9) corn 10% GMO, made from ERM BF433d (100 copies/µl) 0.15 ml Genomic DNA LLCotton25 (ACS-GHØØ1-3) (100 copies/µl) 0.15 ml Genomic DNA MON1445 cotton (MON-Ø1445-2) (100 copies/µl) 0.15 ml Genomic DNA MON15985 cotton (MON ) (100 copies/µl) 0.15 ml Genomic DNA MON531 cotton (MON-ØØ531-6) (100 copies/µl) 0.15 ml Genomic DNA MON cotton (MON ) (100 copies/µl) 0.15 ml Genomic DNA x (Widestrike) cotton Plasmid DNA pgse625 (FP967 flax; EURL method) (CDC-FLØØ1-2) Plasmid DNA pgse644 (Sunup papaya) (e.g. for LMBG 64 PCR and RT-PCR methods) (100 copies/µl) 0.15 ml (100 copies/µl) 0.15 ml (100 copies/µl) 0.15 ml Genomic DNA Sunup papaya (event 55-1) (100 copies/µl) 0.15 ml Plasmid DNA pgse28 (Bt63 rice, EURL recommended method) Plasmid DNA pgse219 (LL62 rice, ACS-OSØØ2-5), for use with EURL detection method (100 copies/µl) 0.15 ml (100 copies/µl) 0.15 ml Plasmid DNA pgse220 (LL601 rice, EURL method) (100 copies/µl) 0.15 ml 17/30

18 Cat # Item Packaging Control DNA GMOs (continued) Genomic DNA MS8 rapeseed (ACS-BNØØ5-8) (100 copies/µl) 0.15 ml Genomic DNA Rf3 rapeseed (ACS-BNØØ3-6) (100 copies/µl) 0.15 ml Genomic DNA RT73 rapeseed (MON-ØØØ73-7) (100 copies/µl) 0.15 ml Genomic DNA T45 rapeseed (ACS-BNØØ8-2) (100 copies/µl) 0.15 ml Genomic DNA DAS soy 10% GMO, made from ERM BF432d Genomic DNA DAS soy 10% GMO, made from ERM BF436e (100 copies/µl) 0.15 ml (100 copies/µl) 0.15 ml Genomic DNA A soy (ACS-GMØØ5-3) (100 copies/µl) 0.15 ml Genomic DNA A soy (ACS-GMØØ6-4) (100 copies/µl) 0.15 ml Genomic DNA CV127 soy (BPS-CV127-9) (100 copies/µl) 0.15 ml Genomic DNA DP soy (DP-3Ø5423-1) 10% GMO, made from CRM BF426d Genomic DNA DP soy (DP-356Ø43-5) 10% GMO, made from CRM BF425d (100 copies GMO/µL) 0.15 ml (100 copies GMO/µL) 0.15 ml Genomic DNA MON87701 soy (MON-877Ø1-2) (100 copies/µl) 0.15 ml Genomic DNA MON89788 soy (MON ) (100 copies/µl) 0.15 ml Genomic DNA Roundup Ready soy (GTS , MON-Ø4Ø32-6) Genomic DNA H7-1 sugarbeet (KM-ØØØ71-4) 100% GMO, from ERM BF419b (100 copies/µl) 0.15 ml (100 copies/µl) 0.15 ml Genomic DNA A soy (ACS-GMØØ5-3) Genomic DNA A soy (ACS-GMØØ6-4) Genomic DNA CV127 soy (BPS-CV127-9) Genomic DNA MON87701 soy (MON-877Ø1-2) Genomic DNA MON89788 soy (MON ) Genomic DNA Roundup Ready Soy (GTS , MON-Ø4Ø32-6) Genomic DNA DP soy (DP-3Ø5423-1), (10% GMO, made from CRM BF426d ) Genomic DNA DP soy (DP-356Ø43-5), (10% GMO, made from CRM BF425d ) (10,000 copies/µl) 0.15 ml (10,000 copies/µl) 0.15 ml (10,000 copies/µl) 0.15 ml (10,000 copies/µl) 0.15 ml (10,000 copies/µl) 0.15 ml (10,000 copies/µl) 0.15 ml (10,000 GMO copies/µl) 0.15 ml (10,000 GMO copies/µl) 0.15 ml 18/30 Kits Product List, 2016 V4

19 Cat # Item Packaging Control DNAs GMOs (continued) Control DNA for real-time PCR detection of GMOs in concentrations close to the LOD level Genomic DNA MON810 maize (MON-ØØ81Ø-6) (20 copies GMO/µL), 20 µl Genomic DNA MON810 maize (MON-ØØ81Ø-6) (20 c. GMO/µL), 10 x 20 µl Genomic DNA T25 maize (ACS-ZMØØ3-2) (20 copies GMO/µL), 20 µl Genomic DNA T25 maize (ACS-ZMØØ3-2) (20 c. GMO/µL), 10 x 20 µl Genomic DNA RT73 rapeseed (MON-ØØØ73-7) (20 copies GMO/µL), 20 µl Genomic DNA RT73 rapeseed (MON-ØØØ73-7) (20 c. GMO/µL), 10 x 20 µl Genomic DNA MS8 rapeseed (ACS-BNØØ5-8) (20 copies GMO/µL), 20 µl Genomic DNA MS8 rapeseed (ACS-BNØØ5-8) (20 c. GMO/µL), 10 x 20 µl Genomic DNA Rf3 rapeseed (ACS-BNØØ3-6) (20 copies GMO/µL), 20 µl Genomic DNA Rf3 rapeseed (ACS-BNØØ3-6 (20 c. GMO/µL), 10 x 20 µl Genomic DNA LL62 rice (ACS-OSØØ2-5) (20 copies GMO/µL), 20 µl Genomic DNA LL62 rice (ACS-OSØØ2-5) (20 c. GMO/µL), 10 x 20 µl Genomic DNA MON soy (MON-Ø4Ø32-6) (20 copies GMO/µL), 20 µl Genomic DNA MON soy (MON-Ø4Ø32-6) (20 c. GMO/µL), 10 x 20 µl Control DNAs Plant Species Genomic DNA Almond (Prunus dulcis) (100 copies/µl) 0.15 ml] Genomic DNA Brazil Nut (Bertholletia excelsa) (100 copies/µl) 0.15 ml Genomic DNA Corn/Maize (Zea mays) (100 copies/µl) 0.15 ml Genomic DNA Cotton (Gossypium) (100 copies/µl) 0.15 ml Genomic DNA Lupine (Lupinus albus) (100 copies/µl) 0.15 ml Genomic DNA Rapeseed/Canola (Brassica napus) (100 copies/µl) 0.15 ml Genomic DNA Rice (Oryza sativa) (100 copies/µl) 0.15 ml Genomic DNA Sesame (Sesamum indicum) (100 copies/µl) 0.15 ml Genomic DNA Soy (Glycine max) (100 copies/µl) 0.15 ml Genomic DNA Alfalfa (Medicago sativa) (10 ng/µl) 0.15 ml Genomic DNA Squash (Cucurbita) (10 ng/µl) 0.15 ml Genomic DNA Papaya (Carica papaya) (10 ng/µl) 0.15 ml Genomic DNA Soy (Glycine max) (10,000 copies/µl) 0.15 ml 19/30

20 Animal Species: Qualitative Real-time PCR Cat # Item Description DNAnimal Kits The DNAnimal product line enables you to identify animal species in your sample. If not specified otherwise, the kits can be used on both, high ROX AND low ROX real-time cyclers. See also our animal species control DNA on the following pages DNAnimal Screen Halal IPC (LR) Kit for simultaneous RT-PCR detection of pork and horse/donkey DNA with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/MX3000P, AriaMX, CFX96 and LC DNAnimal Screen Tetrapod IPC Kit for detection of mammalian and avian DNA with IPC; reptiles, amphibians and fish are not detected. Validated on ABI7900, ABI7500/Fast, MX3005P/MX3000P and LC DNAnimal Screen Ruminant IPC Kit for detection of ruminant DNA with IPC, validated on ABI7900, ABI7500/Fast, MX3005P/ MX3000P, AriaMX, CFX96 and LC DNAnimal Screen Fish IPC Kit for detection of fish DNA, with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/MX3000P and AriaMX DNAnimal Screen Bird IPC Kit for detection of avian DNA, with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/MX3000P and LC DNAnimal Ident Beef IPC Kit for identification of bovine DNA, with IPC (detects besides Bos taurus also bison and different Bos species). Validated on ABI7900, ABI7500/Fast, MX3005P/MX3000P and LC DNAnimal Ident Pork IPC Kit for identification of porcine DNA (both, Sus scrofa domestica and scrofa), with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/MX3000P and LC DNAnimal Ident Meat IPC Kit for identification of bovine and porcine DNA, with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/MX3000P and LC DNAnimal Ident Chicken IPC (LR) Kit for identification of chicken (Gallus gallus) DNA, with IPC. Validated on ABI7500/Fast, MX3005P/ MX3000P, AriaMX, CFX96 and LC DNAnimal Ident Duck IPC Kit for identification of duck (Anas platyrhychos and Cairina moschata) DNA, with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/ MX3000P and LC mulitplex real-time PCR reactions, all reagents and controls included, for up to 44 samples 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR reactions, all reagents and controls included, for up to 46 samples 96 real-time PCR reactions, all reagents and controls included, for up to 46 samples 2x48 RT-PCR rxns, all reagents and positive control DNA included 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 20/30 Kits Product List, 2016 V4

21 Cat # Item Description DNAnimal Kits (continued) DNAnimal Ident Goose IPC Kit for identification of goose DNA, with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/ MX3000P, AriaMX, CFX96 and LC DNAnimal Ident Turkey IPC Kit for identification of turkey DNA, with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/ MX3000P and LC480. DNAnimal Ident Goat IPC Kit for identification of goat DNA, with IPC. Validated on ABI7900, ABI7500/Fast, MX3005P/ MX3000P and LC DNAnimal Ident Horse IPC (LR) Kit for identification of horse DNA, with IPC. Validated on ABI7500/Fast, MX3005P/MX3000P, AriaMX and CFX DNAnimal Ident Water Buffalo IPC (LR) Kit for identification of water buffalo DNA, with IPC. Validated on ABI7500/Fast, MX3005P/MX3000P, AriaMX, CFX96 and LC DNAnimal Ident (LR) Donkey Kit for identification of donkey DNA. Validated on ABI7500/Fast and MX3005P/MX3000P. 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR rxns, all reagents and controls included, for up to 46 samples 96 real-time PCR reactions, all reagents and controls included, for up to 46 samples 96 real-time PCR reactions, all reagents and controls included, for up to 46 samples 96 real-time PCR reactions, all reagents and controls included, for up to 46 samples 21/30

22 Animal Species: Meat Standards and Control DNA Cat # Item Packaging Meat Standards The following meat standards can be used i.e. for threshold testing or method validation: % w/w horse in beef meat standard i.e. for threshold testing according to EURL-AP % w/w pork in beef meat standard i.e. for threshold testing ,1% w/w pork in beef meat standard i.e. for threshold testing % w/w beef in pork meat standard i.e. for beef threshold testing 2,0 +/- 0,1 g minced meat, frozen 2,0 +/- 0,1 g minced meat, frozen 2,0 +/- 0,1 g minced meat, frozen 2,0 +/- 0,1 g minced meat, frozen Control DNAs Animal Species (ng/µl) Genomic DNA Atlantic Cod (Gadus morhua) 0.2 ml [1 ng/µl] Genomic DNA Atlantic Halibut (Hippoglossus hippoglossus) 0.2 ml [1 ng/µl] Genomic DNA Cat (Felis silvestris) 0.2 ml [1 ng/µl] Genomic DNA Cattle (Bos taurus) 0.2 ml [1 ng/µl] Genomic DNA Cattle (Bos taurus) 150 µl [10 ng/µl] Genomic DNA Chicken (Gallus gallus) 0.2 ml [1 ng/µl] Genomic DNA Dog (Canis lupus familiaris) 0.2 ml [1 ng/µl] Genomic DNA Duck 0.2 ml [1 ng/µl] Genomic DNA Fallow Deer (Dama dama) 0.2 ml [1 ng/µl] Genomic DNA Goat (Capra hircus) 0.2 ml [1 ng/µl] Genomic DNA Goose (Anser) 0.2 ml [1 ng/µl] Genomic DNA Hare (Lepus europaeus) 0.2 ml [1 ng/µl] Genomic DNA Horse (Equus caballus) 0.2 ml [1 ng/µl] Genomic DNA Human (Homo sapiens) 0.2 ml [1 ng/µl] Genomic DNA Kangaroo (Macropus giganteus) 0.2 ml [1 ng/µl] Genomic DNA Ostrich (Struthio camelus) 0.2 ml [1 ng/µl] Genomic DNA Pig (Sus scrofa domestica) 0.2 ml [1 ng/µl] Genomic DNA Rabbit (Oryctolagus cuniculus) 0.2 ml [1 ng/µl] Genomic DNA Red Deer (Cervus elaphus) 0.2 ml [1 ng/µl] Genomic DNA Roe Deer (Capreolus capreolus capreolus) 0.2 ml [1 ng/µl] Genomic DNA Saithe/Pollock (Pollachius virens) 0.2 ml [1 ng/µl] Genomic DNA Sika Deer (Cervus nippon) 0.2 ml [1 ng/µl] Genomic DNA Sheep (Ovis aries) 0.2 ml [1 ng/µl] Genomic DNA Springbok (Antidorcas marsupialis) 0.2 ml [1 ng/µl] Genomic DNA Turkey (Meleagris gallopavo) 0.2 ml [1 ng/µl] Genomic DNA Water Buffalo (Bubalus bubalis) 0.2 ml [1 ng/µl] Genomic DNA Water Buffalo (Bubalus bubalis) 150 µl [10 ng/µl] Genomic DNA Wild Boar (Sus scrofa scrofa) 0.2 ml [1 ng/µl] 22/30 Kits Product List, 2016 V4

23 Cat # Item Packaging Control DNAs Animal Species (copies/µl) Genomic DNA Atlantic Halibut (Hippoglossus hippoglossus) 150 µl [100 copies/µl] Genomic DNA Bison (Bison bison) 150 µl [100 copies/µl] Genomic DNA Chamoise (Rupicapra rupicapra) 150 µl [100 copies/µl] Genomic DNA Chicken (Gallus gallus) 150 µl [100 copies/µl] Genomic DNA Donkey (Equus asinus) 150 µl [100 copies/µl] Genomic DNA Duck 150 µl [100 copies/µl] Genomic DNA Gemsbok (Oryx gazella) 150 µl [100 copies/µl] Genomic DNA Gnu (Connochaetes) 150 µl [100 copies/µl] Genomic DNA Goat (Capra hircus) 150 µl [100 copies/µl] Genomic DNA Horse (Equus caballus) 150 µl [100 copies/µl] Genomic DNA Kudu (Tragelaphus) 150 µl [100 copies/µl] Genomic DNA Pig (Sus scrofa domestica) 150 µl [100 copies/µl] Genomic DNA Pigeon (Columba livia) 150 µl [100 copies/µl] Genomic DNA Quail (Coturnix coturnix) 150 µl [100 copies/µl] Genomic DNA Rat (Rattus rattus) 150 µl [100 copies/µl] Genomic DNA Sheep (Ovis aries) 150 µl [100 copies/µl] Genomic DNA Turkey (Meleagris gallopavo) 150 µl [100 copies/µl] Genomic DNA Water Buffalo (Bubalus bubalis) 150 µl [100 copies/µl] Genomic DNA Yak (Bos mutus) 150 µl [100 copies/µl] 23/30

24 Food pathogens: Real-time PCR and ELISA Cat # Item Description BACGene Real-time PCR Kits The BACGene product line is designed for a fast DNA extraction and real-time PCR identification of food pathogens BACGene Salmonella spp. Salmonella spp. kit for 96 DNA extractions and RT-PCR detections. AOAC Performance tested certified and AFNOR FN-certified alternative method for Salmonella spp. detection according to EN ISO from 25 g samples of all foods including meat/meat products, milk and dairy products, produces (vegetables, fruits and related products), Eggs and egg products, fish and seafood products, 25 g of feed (including pet food), 375 g of pet food, 375 g of milk powders & infant formula (with and without probiotics) and environmental samples. For use with Agilent AriaMX and Bio-Rad CFX96 Touch. Bioline ELISA Kits The Bioline pathogen ELISA test kits are designed for fast and reliable identification of food pathogens Bioline Salmonella ELISA OPTIMA test Bioline Salmonella ELISA OPTIMA test (5x) Bioline Salmonella Optima ELISA test kit, Cat. no. S96-1, 96 well ELISA plate AOAC Performance tested certified Bioline Salmonella Optima, Cat. no. S96-5, 5 x 96 well ELISA plate AOAC Performance tested certified Bioline Listeria ELISA test Bioline Listeria ELISA test kit, Cat. no. L96-1, 96 well ELISA plate Bioline Listeria ELISA test (5x) Bioline Listeria ELISA test kit, Cat. no. L96-5, 5 x 96 well ELISA plate Ordering and Shipping Information Purchase orders can be placed i.e. by phone or by to kits@eurofins.de. In order to avoid any delay in order processing, please clearly indicate delivery and invoice adress. For European countries, please include your VAT with your first order. Delivery time depends on stock availability and shipping destination and is usually 1 to 10 working days. An order confirmation with estimated shipping date will be provided. At the time of delivery, we guarantee a shelf life of six months or more for our products. In case of doubt please enquire for available lots and expiration dates. Freight charges depend on destination and number/volume of kits ordered. Please enquire for your destination (kits@eurofins.de). 24/30 Kits Product List, 2016 V4

Developments with respect to GMO analysis: Experience from Brazil

Developments with respect to GMO analysis: Experience from Brazil 2nd International Workshop on Harmonisation of GMO Detection and Analysis for Central and South America Developments with respect to GMO analysis: Experience from Brazil Maria Regina Branquinho, INCQS/MS

More information

CENÍK. genesig detection kits (see website for full current listing)

CENÍK. genesig detection kits (see website for full current listing) CENÍK obchodní divize Forenzní DNA servis, s.r.o. Budínova 2, 180 81 Praha 8, CZE tel/fax: 233 931 123, GSM: 731 503 250 e-mail: amplicon@dna.com.cz www.amplicon.cz Cena chlazených a mražených produktů

More information

quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476

quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476 BioScience quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476 This method describes a Real-time semi-quantitative TaqMan PCR procedure for the determination of

More information

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to

More information

Example, Status and experience from Brazil PART II

Example, Status and experience from Brazil PART II International Workshop on Harmonisation of GMO Detection and Analysis for Central and South America Example, Status and experience from Brazil PART II Paola Cardarelli-Leite, Ph.D. Maria Regina Branquinho,

More information

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube User Manual CelluLyser Lysis and cdna Synthesis Kit Version 1.4 Oct 2012 From cells to cdna in one tube CelluLyser Lysis and cdna Synthesis Kit Table of contents Introduction 4 Contents 5 Storage 5 Additionally

More information

FastLine cell cdna Kit

FastLine cell cdna Kit 1. FastLine cell cdna Kit For high-speed preparation of first-strand cdna directly from cultured cells without RNA purification www.tiangen.com RT100701 FastLine cell cdna Kit Cat. no. KR105 Kit Contents

More information

Factors Influencing Multiplex Real-Time PCR

Factors Influencing Multiplex Real-Time PCR APPLICATION NOTE Multiplex Real-Time PCR Factors Influencing Multiplex Real-Time PCR Introduction Multiplex PCR is the simultaneous amplification of more than one target sequence in a single reaction [1].

More information

Annex to the Accreditation Certificate D-PL-13372-01-00 according to DIN EN ISO/IEC 17025:2005

Annex to the Accreditation Certificate D-PL-13372-01-00 according to DIN EN ISO/IEC 17025:2005 Deutsche Akkreditierungsstelle GmbH German Accreditation Body Annex to the Accreditation Certificate D-PL-13372-01-00 according to DIN EN ISO/IEC 17025:2005 Period of validity: 26.03.2012 to 25.03.2017

More information

MystiCq microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C

MystiCq microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C Product Description The microrna cdna Synthesis Mix has been designed to easily convert micrornas into cdna templates for qpcr

More information

Plant Biotechnology Pipeline

Plant Biotechnology Pipeline Corn 4 th -Generation Herbicide Tolerance 3 rd -Generation Herbicide Tolerance Multiple Mode Enlist : 2,4-D & FOP 4 th -Generation Below- Ground Insect Protection 3 rd -Generation Above- Ground Insect

More information

RealStar HBV PCR Kit 1.0 11/2012

RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 For research use only! (RUO) Product No.: 201003 96 rxns INS-201000-GB-02 Store at -25 C... -15 C November 2012 altona Diagnostics GmbH Mörkenstraße

More information

Seed companies experiences facing LLP issues

Seed companies experiences facing LLP issues Associação Brasileira de Sementes e Mudas Seed companies experiences facing LLP issues Paulo Campante III Seed Congress of the Americas - Santiago, Chile September 28-29, 2011 Associação Brasileira de

More information

Authentication of Basmati rice using SSR-PCR and QIAxcel Advanced

Authentication of Basmati rice using SSR-PCR and QIAxcel Advanced Application Note Authentication of Basmati rice using SSR-PCR and QIAxcel Advanced R. Cassier ADGENE Laboratoire, Thury Harcourt, France Introduction Basmati is one of the most popular types of rice in

More information

ABSTRACT. Promega Corporation, Updated September 2008. http://www.promega.com/pubhub. 1 Campbell-Staton, S.

ABSTRACT. Promega Corporation, Updated September 2008. http://www.promega.com/pubhub. 1 Campbell-Staton, S. A Modified Wizard SV Genomic DNA Purification System Protocol to Purify Genomic DNA... A Modified Wizard SV Genomic DNA Purification System Protocol to Purify Genomic DNA from Shed Reptile Skin ABSTRACT

More information

User Bulletin #2 ABI PRISM 7700 Sequence Detection System

User Bulletin #2 ABI PRISM 7700 Sequence Detection System User Bulletin #2 ABI PRISM 7700 Sequence Detection System December 11, 1997 (updated 10/2001) SUBJECT: Relative Quantitation of Gene Expression Introduction Amplification of an endogenous control may be

More information

Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene. genesig Advanced Kit. DNA testing

Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene. genesig Advanced Kit. DNA testing TM Primerdesign Ltd TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

Real-Time Quantitative PCR Instruments and Consumables

Real-Time Quantitative PCR Instruments and Consumables Index Page Sequence Detection Systems 2 Sequence Detection Systems Accessories and Softwares 3 Sequence Detection PCR Reagent Kits 4 Sequence Detection PCR Reagents 9 Sequence Detection Systems Disposables

More information

Epstein Barr Virus (Human Herpes virus 4) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...

Epstein Barr Virus (Human Herpes virus 4) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... TM Primerdesign Ltd TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

Real-time quantitative RT -PCR (Taqman)

Real-time quantitative RT -PCR (Taqman) Real-time quantitative RT -PCR (Taqman) Author: SC, Patti Lab, 3/03 This is performed as a 2-step reaction: 1. cdna synthesis from DNase 1-treated total RNA 2. PCR 1. cdna synthesis (Advantage RT-for-PCR

More information

Quantitative PCR Systems

Quantitative PCR Systems QUANTITATIVE PCR Quantitative PCR Systems Our QPCR systems meet your current research needs, offer flexibility for future research goals, and match your budget. HIGH-PERFORMANCE FULL-FEATURED FLEXIBLE

More information

Human Herpes Virus 4 (Epstein Barr)

Human Herpes Virus 4 (Epstein Barr) Techne qpcr test Human Herpes Virus 4 (Epstein Barr) nonglycosylated membrane protein (BNRF1) gene 150 tests For general laboratory and research use only 1 Introduction to Human Herpes Virus 4 (Epstein

More information

Essentials of Real Time PCR. About Sequence Detection Chemistries

Essentials of Real Time PCR. About Sequence Detection Chemistries Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected

More information

RealLine HCV PCR Qualitative - Uni-Format

RealLine HCV PCR Qualitative - Uni-Format Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013

More information

Absolute Quantitation Using Standard Curve Getting Started Guide

Absolute Quantitation Using Standard Curve Getting Started Guide Applied Biosystems 7900HT Fast Real-Time PCR System Absolute Quantitation Using Standard Curve Getting Started Guide Introduction Designing an AQ Experiment Preparing the Samples and Reaction Plate Creating

More information

NSF International Non-GMO Project Verification Guidance Document

NSF International Non-GMO Project Verification Guidance Document NSF International Non-GMO Project Verification Guidance Document Table of Contents > NSF Document Overview > Inputs > Input Compliance Documentation > Segregation > Testing Requirement > Acceptable IP

More information

Food Sources of Vitamin B12

Food Sources of Vitamin B12 Food Sources of Vitamin B12 Information About Vitamin B12 You need vitamin B12 to form DNA, make healthy blood cells and keep nerves working properly. Low levels of vitamin B12 can cause pernicious anemia.

More information

Soybean Seeds Sampling and DNA Extraction. Report on the Validation of a DNA Extraction Method from Soybean Seeds

Soybean Seeds Sampling and DNA Extraction. Report on the Validation of a DNA Extraction Method from Soybean Seeds Soybean Seeds Sampling and DNA Extraction Report on the Validation of a DNA Extraction Method from Soybean Seeds 14 May 2007 Directorate General-Joint Research Centre Institute for Health and Consumer

More information

Highly specific and sensitive quantitation

Highly specific and sensitive quantitation PRODUCT ULLETIN SYR Select Master Mix SYR Select Master Mix Highly specific and sensitive quantitation SYR Select Master Mix offers advanced performance at an affordable price. SYR Select Master Mix is

More information

Quantification of Mycobacterium Tuberculosis. 150 tests

Quantification of Mycobacterium Tuberculosis. 150 tests Quantification of Mycobacterium Tuberculosis rep 13E12 For general laboratory and research use only 150 tests Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis is the bacterium that

More information

Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR. Protocol

Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR. Protocol Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR Protocol 31 January 2011 Joint Research Centre Institute for Health and Consumer Protection Molecular Biology and Genomics

More information

TaqMan Fast Advanced Master Mix. Protocol

TaqMan Fast Advanced Master Mix. Protocol TaqMan Fast Advanced Master Mix Protocol For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED

More information

GMO Risk Assessment - EU experiences and recent developments

GMO Risk Assessment - EU experiences and recent developments A future task in good hands GMO Risk Assessment - EU experiences and recent developments Beatrix Tappeser Head, Biosafety Division, BfN, Germany ENSSER - EEA Symposium on Science & Society 'Integrity of

More information

Mondelēz International Supply Chain Management for Materials of Potential GMO Origin in EU and Eastern Europe, Middle East and Africa (EEMEA) Regions

Mondelēz International Supply Chain Management for Materials of Potential GMO Origin in EU and Eastern Europe, Middle East and Africa (EEMEA) Regions Mondelēz International Supply Chain Management for Materials of Potential GMO Origin in EU and Eastern Europe, Middle East and Africa (EEMEA) Regions Updated July 6th, 2015 Mondelēz International Corporate

More information

PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR. Results Interpretation Guide

PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR. Results Interpretation Guide PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR Results Interpretation Guide Pathogen Detection Systems by Real Time PCR Microbial offers real time PCR based systems for the detection of pathogenic bacteria

More information

Lyme Disease RecA gene. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... 150 tests. Primerdesign Ltd.

Lyme Disease RecA gene. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... 150 tests. Primerdesign Ltd. TM Primerdesign Ltd TM Primerdesign Ltd Lyme Disease RecA gene genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use only 1 Introduction

More information

Application Guide... 2

Application Guide... 2 Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue Application Guide... 2 I. Description... 2 II. Product Components... 2 III. Materials to be Supplied

More information

Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for Use in Quantitative PCR

Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for Use in Quantitative PCR Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for Use in Quantitative PCR Overview Genomic DNA (gdna) and plasmids containing cloned target sequences are commonly used as standards

More information

QuantiFast Multiplex PCR Handbook

QuantiFast Multiplex PCR Handbook May 2011 QuantiFast Multiplex PCR Handbook QuantiFast Multiplex PCR Kit with master mix containing ROX passive reference dye QuantiFast Multiplex PCR +R Kit with separate tube of ROX passive reference

More information

Eppendorf PCR Consumables Compatibility Guide for PCR and qpcr Cyclers

Eppendorf PCR Consumables Compatibility Guide for PCR and qpcr Cyclers APPLICATION NOTE No. 266 I November 2012 Eppendorf PCR Consumables Compatibility Guide for PCR and qpcr Cyclers Rafal Grzeskowiak 1, Sandrine Hamels 2, Beate Riekens 1 1 Eppendorf AG, Hamburg, Germany;

More information

Human Herpes Virus 1 (Herpes simplex type 1) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...

Human Herpes Virus 1 (Herpes simplex type 1) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... TM Primerdesign Ltd TM Primerdesign Ltd Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

Dengue Virus subtypes 1,2 3 and 4. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... 3 Untranslated Region (3 UTR) 150 tests

Dengue Virus subtypes 1,2 3 and 4. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... 3 Untranslated Region (3 UTR) 150 tests TM Primerdesign Ltd TM Primerdesign Ltd Dengue Virus subtypes 1,2 3 and 4 3 Untranslated Region (3 UTR) genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory

More information

Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing European Network of GMO Laboratories (ENGL)

Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing European Network of GMO Laboratories (ENGL) Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing European Network of GMO Laboratories (ENGL) 13 October 2008 Date of application: 13 April 2009 INTRODUCTION The scope

More information

Genomic DNA Extraction Kit INSTRUCTION MANUAL

Genomic DNA Extraction Kit INSTRUCTION MANUAL Genomic DNA Extraction Kit INSTRUCTION MANUAL Table of Contents Introduction 3 Kit Components 3 Storage Conditions 4 Recommended Equipment and Reagents 4 Introduction to the Protocol 4 General Overview

More information

Validating Microarray Data Using RT 2 Real-Time PCR Products

Validating Microarray Data Using RT 2 Real-Time PCR Products Validating Microarray Data Using RT 2 Real-Time PCR Products Introduction: Real-time PCR monitors the amount of amplicon as the reaction occurs. Usually, the amount of product is directly related to the

More information

Lyme Disease. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... RecA gene. 150 tests. Primerdesign Ltd.

Lyme Disease. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... RecA gene. 150 tests. Primerdesign Ltd. TM Primerdesign Ltd TM Primerdesign Ltd Lyme Disease RecA gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use only 1 Introduction

More information

Lyme Disease. RecA gene. 150 tests. Quantification of Lyme Disease genomes. Advanced kit handbook HB10.03.07

Lyme Disease. RecA gene. 150 tests. Quantification of Lyme Disease genomes. Advanced kit handbook HB10.03.07 Techne qpcr test Lyme Disease RecA gene 150 tests For general laboratory and research use only 1 Introduction to Lyme Disease Lyme disease is an infectious disease caused mainly by three species of bacteria

More information

ID kit. imegen Anchovies II. and E. japonicus) DNA detection by. User manual. Anchovies species (E. encrasicolus. sequencing.

ID kit. imegen Anchovies II. and E. japonicus) DNA detection by. User manual. Anchovies species (E. encrasicolus. sequencing. User manual imegen Anchovies II ID kit Anchovies species (E. encrasicolus and E. japonicus) DNA detection by sequencing Reference: Made in Spain The information in this guide is subject to change without

More information

BacReady TM Multiplex PCR System

BacReady TM Multiplex PCR System BacReady TM Multiplex PCR System Technical Manual No. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI Detailed Experimental

More information

SYBR Green Realtime PCR Master Mix -Plus-

SYBR Green Realtime PCR Master Mix -Plus- Instruction manual SYBR Green Realtime PCR Master Mix -Plus- 0810 F0925K SYBR Green Realtime PCR Master Mix -Plus- Contents QPK-212T 1mLx1 QPK-212 1mLx5 Store at -20 C, protected from light [1] Introduction

More information

Effective Financing Statement (EFS)

Effective Financing Statement (EFS) Fax 307.777.5339 For Office Use Only Effective Financing Statement (EFS) 1. Contact and Send Acknowledgment To: a. Name and Phone of Contact at Filer (optional) b. PAD Account # to be Charged c. Send Acknowledgement

More information

TITRATION OF raav (VG) USING QUANTITATIVE REAL TIME PCR

TITRATION OF raav (VG) USING QUANTITATIVE REAL TIME PCR Page 1 of 5 Materials DNase digestion buffer [13 mm Tris-Cl, ph7,5 / 5 mm MgCl2 / 0,12 mm CaCl2] RSS plasmid ptr-uf11 SV40pA Forward primer (10µM) AGC AAT AGC ATC ACA AAT TTC ACA A SV40pA Reverse Primer

More information

Genomic DNA detection assay

Genomic DNA detection assay Genomic DNA detection assay Detection of genomic DNA by real-time PCR Contents CTRL Internal controls and gdna detection Contents Kit Contents 3 Reagents and Equipment to Be Supplied by User 3 Kit Storage

More information

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011 Page 0 INSTRUCTION MANUAL Catalog Nos. D4010 & D4011 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)dna, etc.)

More information

Report on the Validation of a "Dellaporta- Derived" Method for DNA Extraction from Ground Maize Grains and Seeds

Report on the Validation of a Dellaporta- Derived Method for DNA Extraction from Ground Maize Grains and Seeds Report on the Validation of a "Dellaporta- Derived" Method for DNA Extraction from Ground Maize Grains and Seeds 15 October 2008 Joint Research Centre Institute for Health and Consumer Protection Biotechnology

More information

Path-ID Multiplex One-Step RT-PCR Kit

Path-ID Multiplex One-Step RT-PCR Kit USER GUIDE Path-ID Multiplex One-Step RT-PCR Kit TaqMan probe-based multiplex one-step real-time RT-PCR detection of RNA targets Catalog Numbers 4428206, 4428207, 4440022 Publication Part Number 4440907

More information

DNA Sequencing Setup and Troubleshooting

DNA Sequencing Setup and Troubleshooting DNA Sequencing Setup and Troubleshooting Lara Cullen, PhD Scientific Applications Specialist Australia and New Zealand Reviewing Sequencing Data Review the Electropherogram Review the Raw Data (Signal

More information

Food Sources of Omega-3 Fats

Food Sources of Omega-3 Fats Food Sources of Omega-3 Fats Information about Omega-3 Fats Omega-3 fats have many functions in our body and are important for good health. There are three kinds of omega-3 fats: o ALA (alpha-linolenic

More information

Introduction To Real Time Quantitative PCR (qpcr)

Introduction To Real Time Quantitative PCR (qpcr) Introduction To Real Time Quantitative PCR (qpcr) SABiosciences, A QIAGEN Company www.sabiosciences.com The Seminar Topics The advantages of qpcr versus conventional PCR Work flow & applications Factors

More information

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-)

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608)

More information

Taqman TCID50 for AAV Vector Infectious Titer Determination

Taqman TCID50 for AAV Vector Infectious Titer Determination Page 1 of 8 Purpose: To determine the concentration of infectious particles in an AAV vector sample. This process involves serial dilution of the vector in a TCID50 format and endpoint determination through

More information

The GeneAmp PCR System 9700. Results you can trust. A PCR platform you can grow with. GeneAmp. PCR System 9700

The GeneAmp PCR System 9700. Results you can trust. A PCR platform you can grow with. GeneAmp. PCR System 9700 The GeneAmp PCR System 9700 Results you can trust. A PCR platform you can grow with. GeneAmp PCR System 9700 The GeneAmp PCR System 9700 fits your lab bench, your applications and your budget. The GeneAmp

More information

How-to Eat Kosher and Maintain a Healthy Diet. By David Braun

How-to Eat Kosher and Maintain a Healthy Diet. By David Braun How-to Eat Kosher and Maintain a Healthy Diet By David Braun What is Kosher? Foods that are permitted to be eaten under Jewish dietary law (Kashrut) Includes restrictions pertaining to: types of animals

More information

mircute mirna qpcr Detection Kit (SYBR Green)

mircute mirna qpcr Detection Kit (SYBR Green) mircute mirna qpcr Detection Kit (SYBR Green) For detection of mirna using real-time RT-PCR (SYBR Green I) www.tiangen.com QP110302 mircute mirna qpcr Detection Kit (SYBR Green) Kit Contents Cat. no. FP401

More information

Procedures For DNA Sequencing

Procedures For DNA Sequencing Procedures For DNA Sequencing Plant-Microbe Genomics Facility (PMGF) Ohio State University 420 Biological Sciences Building 484 W. 12th Ave., Columbus OH 43210 Telephone: 614/247-6204 FAX: 614/292-6337

More information

Product List 2012. Cat nr Product Reactions Listprice ( ) ex VAT)

Product List 2012. Cat nr Product Reactions Listprice ( ) ex VAT) Cat nr Product Reactions Listprice ( ) ex VAT) Q-PCR kits Pathogens ID12100-50 Quickblue DNA extraction kit 50 165 ID12100-250 Quickblue DNA extraction kit 250 775 ID12100-1000 Quickblue DNA extraction

More information

CompleteⅡ 1st strand cdna Synthesis Kit

CompleteⅡ 1st strand cdna Synthesis Kit Instruction Manual CompleteⅡ 1st strand cdna Synthesis Kit Catalog # GM30401, GM30402 Green Mountain Biosystems. LLC Web: www.greenmountainbio.com Tel: 800-942-1160 Sales: Sales@ greenmountainbio.com Support:

More information

Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr

Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr User Manual Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories,

More information

Attivita del Laboratorio Europeo di Riferimento sugli OGM (EURL-GMFF) in supporto al sistema di controllo ufficiale degli Stati Membri

Attivita del Laboratorio Europeo di Riferimento sugli OGM (EURL-GMFF) in supporto al sistema di controllo ufficiale degli Stati Membri Roma IZSLT 30 Novembre 2010 1 Attivita del Laboratorio Europeo di Riferimento sugli OGM (EURL-GMFF) in supporto al sistema di controllo ufficiale degli Stati Membri Marco Mazzara Joint Research Centre,

More information

Gene Expression Assays

Gene Expression Assays APPLICATION NOTE TaqMan Gene Expression Assays A mpl i fic ationef ficienc yof TaqMan Gene Expression Assays Assays tested extensively for qpcr efficiency Key factors that affect efficiency Efficiency

More information

Fluorescent dyes for use with the

Fluorescent dyes for use with the Detection of Multiple Reporter Dyes in Real-time, On-line PCR Analysis with the LightCycler System Gregor Sagner, Cornelia Goldstein, and Rob van Miltenburg Roche Molecular Biochemicals, Penzberg, Germany

More information

altona altona RealStar Clostridium difficile PCR Kit 1.0 always a drop ahead. 11/2012 altona Diagnostics GmbH Moerkenstr. 12 22767 Hamburg Germany

altona altona RealStar Clostridium difficile PCR Kit 1.0 always a drop ahead. 11/2012 altona Diagnostics GmbH Moerkenstr. 12 22767 Hamburg Germany altona DIAGNOSTICS altona DIAGNOSTICS RealStar Clostridium difficile PCR Kit 1.0 11/2012 altona Diagnostics GmbH Moerkenstr. 12 22767 Hamburg Germany phone +49 40 548 06 76-0 fax +49 40 548 06 76-10 e-mail

More information

DyNAmo cdna Synthesis Kit for qrt-pcr

DyNAmo cdna Synthesis Kit for qrt-pcr DyNAmo cdna Synthesis Kit for qrt-pcr Instruction manual F- 470S Sufficient for 20 cdna synthesis reactions (20 µl each) F- 470L Sufficient for 100 cdna synthesis reactions (20 µl each) Description...

More information

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual F- 470S 20 cdna synthesis reactions (20 µl each) F- 470L 100 cdna synthesis reactions (20 µl each) Table of contents 1. Description...

More information

RNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr)

RNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr) RNA Extraction and Quantification, Reverse Transcription, and Real-time Preparation of Samples Cells: o Remove media and wash cells 2X with cold PBS. (2 ml for 6 well plate or 3 ml for 6cm plate) Keep

More information

Protein Values in Foods

Protein Values in Foods Protein Values in Foods This document is used as reference material to support the Alternative Health Improvement Center s Newsletter article: Eat your Protein! Stay Healthy! located at http://ahicatlanta.com/articles/eat-your-protein-to-stay-healthy.html.

More information

Speed Matters - Fast ways from template to result

Speed Matters - Fast ways from template to result qpcr Symposium 2007 - Weihenstephan Speed Matters - Fast ways from template to result March 28, 2007 Dr. Thorsten Traeger Senior Scientist, Research and Development - 1 - Overview Ạgenda Fast PCR The Challenges

More information

Life-Science Economics and Policy

Life-Science Economics and Policy Life-Science Economics and Policy Winter Term 2011/12 Dr. Maarten J. Punt Technische Universität München - Weihenstephan maarten.punt@tum.de http://www.wzw.tum.de/aew/ GMO adoption by countries What is

More information

RT-PCR: Two-Step Protocol

RT-PCR: Two-Step Protocol RT-PCR: Two-Step Protocol We will provide both one-step and two-step protocols for RT-PCR. We recommend the twostep protocol for this class. In the one-step protocol, the components of RT and PCR are mixed

More information

Introduction. Preparation of Template DNA

Introduction. Preparation of Template DNA Procedures and Recommendations for DNA Sequencing at the Plant-Microbe Genomics Facility Ohio State University Biological Sciences Building Room 420, 484 W. 12th Ave., Columbus OH 43210 Telephone: 614/247-6204;

More information

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One TM mirna First-Strand cdna Synthesis Kit AMRT-0020 (20 RT reactions), AMRT-0060 (60 RT reactions) Used in combination

More information

Řekněte si o vzorky zdarma!

Řekněte si o vzorky zdarma! strana 1 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od 15.10.2015 Core Reagents for Molecular Biology www.bioline.com Řekněte si o vzorky zdarma! PCR ENZYME & MIXES DNA Polymerases - For

More information

Mir-X mirna First-Strand Synthesis Kit User Manual

Mir-X mirna First-Strand Synthesis Kit User Manual User Manual Mir-X mirna First-Strand Synthesis Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc.

More information

All-in-One First-Strand cdna Synthesis Kit

All-in-One First-Strand cdna Synthesis Kit All-in-One First-Strand cdna Synthesis Kit For reliable first-strand cdna synthesis from all RNA sources Cat. No. AORT-0020 (20 synthesis reactions) Cat. No. AORT-0050 (50 synthesis reactions) User Manual

More information

User Manual. Reference Gene Panel Mouse Probe protocol. Version 1.1 August 2014 For use in quantitative real-time PCR

User Manual. Reference Gene Panel Mouse Probe protocol. Version 1.1 August 2014 For use in quantitative real-time PCR User Manual Reference Gene Panel Mouse Probe protocol Version 1.1 August 2014 For use in quantitative real-time PCR Reference Gene Panel Mouse Table of contents Background 4 Assays included in the panel

More information

All-in-One mirna qrt-pcr Detection System Handbook

All-in-One mirna qrt-pcr Detection System Handbook All-in-One mirna qrt-pcr Detection System Handbook For quantitative detection of mature mirna All-in-One mirna First-Strand cdna Synthesis Kit Cat. No. AMRT-0020 (20 mirna reverse transcription reactions)

More information

QPCR Applications using Stratagene s Mx Real-Time PCR Platform

QPCR Applications using Stratagene s Mx Real-Time PCR Platform QPCR Applications using Stratagene s Mx Real-Time PCR Platform Dan Schoeffner, Ph.D Field Applications Scientist Dan.Schoeffner@Stratagene.com Tech. Services 800-894-1304 Polymerase Chain Reaction Melt

More information

DNA Integrity Number (DIN) For the Assessment of Genomic DNA Samples in Real-Time Quantitative PCR (qpcr) Experiments

DNA Integrity Number (DIN) For the Assessment of Genomic DNA Samples in Real-Time Quantitative PCR (qpcr) Experiments DNA Integrity Number () For the Assessment of Genomic DNA Samples in Real-Time Quantitative PCR (qpcr) Experiments Application Note Nucleic Acid Analysis Author Arunkumar Padmanaban Agilent Technologies,

More information

Absolute Quantification Getting Started Guide

Absolute Quantification Getting Started Guide 5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide Introduction

More information

Relative Quantification Applied Biosystems 7300/7500 Real Time PCR System

Relative Quantification Applied Biosystems 7300/7500 Real Time PCR System 5 3 5 cdna Reverse Primer Oligo d(t) or random hexamer 3 5 cdna Getting Started Guide Relative Quantification Applied Biosystems 7300/7500 Real Time PCR System Introduction and Example RQ Experiment Designing

More information

Methods verification. Transfer of validated methods into laboratories working routine. Dr. Manuela Schulze 1

Methods verification. Transfer of validated methods into laboratories working routine. Dr. Manuela Schulze 1 Methods verification Transfer of validated methods into laboratories working routine Dr. Manuela Schulze 1 1. Introduction 2. Definitions and differences validation verification 3. How to perform verification

More information

Quantification of Hepatitis B Virus. Core Protein Region. 150 tests

Quantification of Hepatitis B Virus. Core Protein Region. 150 tests Quantification of Hepatitis B Virus Core Protein Region For general laboratory and research use only 150 tests Introduction to Hepatitis B Virus Originally known as serum hepatitis, hepatitis B has only

More information

Simple home made diets for dogs and cats

Simple home made diets for dogs and cats Simple home made diets for dogs and cats mince and beef mince if possible. 2. Rolled oats soak an equal weight of dry rolled oats in water overnight. Ie 100g dry rolled oats, soaked in 100ml water =. 3.

More information

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests.

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests. TM Primerdesign Ltd TM Primerdesign Ltd Hepatitis B Virus Core Protein Region genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use

More information

Quantitative Real Time PCR Protocol. Stack Lab

Quantitative Real Time PCR Protocol. Stack Lab Quantitative Real Time PCR Protocol Stack Lab Overview Real-time quantitative polymerase chain reaction (qpcr) differs from regular PCR by including in the reaction fluorescent reporter molecules that

More information

Keeping It Legal: Regulations and Licenses for Growing and Selling Food in Oregon

Keeping It Legal: Regulations and Licenses for Growing and Selling Food in Oregon Keeping It Legal: Regulations and Licenses for Growing and Selling Food in Oregon Adapted from Growing Farms Online, a comprehensive training program for beginning farmers, from the OSU Small Farms Program.

More information

The Danish veterinary preparedness for avian influenza and Newcastle disease

The Danish veterinary preparedness for avian influenza and Newcastle disease The Danish veterinary preparedness for avian influenza and Newcastle disease Sten Mortensen, Veterinary R&D manager, Animal Health Division, Deputy head 19-04-2016 Livestock statistics, Denmark 2015 Species

More information

Patents on Seeds and Animals, Eggs & Bacon, Tomatoes and Melons

Patents on Seeds and Animals, Eggs & Bacon, Tomatoes and Melons Patents on Seeds and Animals, Eggs & Bacon, Tomatoes and Melons November 2011 Dr. Christoph Then www.no-patents-on-seeds.org Info@no-patents-on-seeds.org History of patent law English patent law of 17th

More information

The global pipeline of new GM crops

The global pipeline of new GM crops The global pipeline of new GM crops Implications of asynchronous approval for international trade Authors: Alexander J. Stein and Emilio Rodríguez-Cerezo 2009 EUR 23486 EN The mission of the JRC-IPTS is

More information

HBV PCR detection Kit USER MANUAL

HBV PCR detection Kit USER MANUAL For professional use only HBV PCR detection Kit (PREP-NA DNA/RNA Extraction Kit included) USER MANUAL "DNA-Technology, Research & Production" LLC Russia, 142281, Moscow Region, Protvino, 2 Zheleznodorozhnaya

More information