GMO Risk Assessment - EU experiences and recent developments
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1 A future task in good hands GMO Risk Assessment - EU experiences and recent developments Beatrix Tappeser Head, Biosafety Division, BfN, Germany ENSSER - EEA Symposium on Science & Society 'Integrity of Science under Attack Kopenhagen, March 15-16, 2010
2 Prologue The German Federal Agency for Nature Conservation (BfN) Central scientific authority of the German federal government for national and international nature conservation and landscape management. Regulatory tasks Evaluation and performance of environmental risk assessment Evaluation and development of monitoring plans Evaluation of monitoring data one of the Competent authorities (CAs) under the German Genetic Engineering Act concerned with the regulation of genetically modified organisms on national and EU level lead authority Federal Office for consumer protection an food safety
3 Prologue The German Federal Agency for Nature Conservation (BfN) GMO-related scientific and collaborative tasks: Advancement of scientific tools for the environmental risk assessment Development of scientific concepts and advancement of Quelle: / methods for the monitoring of GMOs Deliver scientific expertise and advise to the Ministry and the Federal States Cooperation with national and international research institutions
4 Topics of this presentation Cultivation in Europe applications and pipeline The current practice of environmental risk assessment Two current examples - MON x and MON The LLP project Conclusions
5 Planned Maize Cultivation in Europe Maize events applied for cultivation in Europe Monsanto YieldGard MON810 Insect resistance (corn borer, lepidopterans) Monsanto Roundup NK603 Herbicide tolerance Ready (to glyphosate) Stack NK603xMON810 Stack NK 603xMON Monsanto YieldGard VT MON88017 * Insect resistance (to coleopterans) Stack MON88017xMON Dow AgroSciences Herculex I 1507 Insect resistance Pioneer Hi-Bred, (to lepidopterans) Dow AgroSciences Herculex RW Insect resistence Pioneer Hi-Bred (to coleopterans) Stack 59122x1507 Stack 59177x1507xNK603 Syngenta Agrisure GT GA21 Herbicide tolerance (to glyphosate) Bayer CropScience T25 Herbicide tolerance Stein & Rodriguez-Cerezo, JRC, 2009 (to glufosinate)
6 GM maize pipeline Maize events authorised in at least one country but not yet commercialised in Europe Monsanto YieldGard VT PRO MON89034 * Insect resistance (to lepidopterans) Monsanto High lysine LY038 * Crop composition (high lysine content) Syngenta n/a 3272 * Crop composition (amylase content) Syngenta Agrisure MIR604 * Insect resistance (to coleopterans) Stein & Rodriguez-Cerezo, JRC, 2009
7 GM maize pipeline GM maize in the advanced R&D pipeline worldwide Monsanto n/a MON87754 Crop composition 2010 (high oleic content) Pioneer Hi-Bred Optimum n/a Insect resistance 2010 AcreMax 1 (to coleopterans) Monsanto n/a MON87460 Abiotic stress tolerance and BASF (to drought) 2012 Dow AgroSciences DHT n/a Herbicide tolerance 2012 n/a (India) n/a cry1ac + Insect resistance 2014 cp4epsp4 herbicide tolerance Syngenta n/a n/a Abiotic stress tolerance 2015 (to drought) BASF Plant Science NutriDense n/a Crop composition 2015 (protein, amino acid and Stein & Rodriguez-Cerezo, JRC, 2009 phytase content)
8 Soybean commercialisation Commercialised soybean events in at least one country Monsanto RoundupReady MON Herbicide tolerance (to glyphosate) Monsanto RoundupReady 2 MON89788 Herbicide tolerance (to glyphosate) Bayer LibertyLink A Herbicide tolerance CropScience (to glufosinate) Bayer LibertyLink A * Herbicide tolerance CropScience (to glufosinate) Pioneer Hi-Bred Optimum GAT * Herbicide tolerance (to ALS inhibitors and glyphosate) Stein & Rodriguez-Cerezo, JRC, 2009
9 Soybean pipeline GM soybeans in the advanced R&D pipeline worldwide Syngenta n/a n/a Nematode resistance 2011 Monsanto Omega-3 MON87769 Crop composition 2012 (stearidonic acid content) Monsanto n/a n/a Herbicide tolerance 2012 (to dicamba) Monsanto n/a n/a Insect resistance and 2013 herbicide tolerance (to glyphosate) Dow AgroSciences DHT n/a Herbicide tolerance 2013 Monsanto Vistive III MON87754 Crop composition 2014 (high oleic content) Syngenta n/a n/a Herbicide tolerance 2014 (to HPPD inhibitors) Bayer CropScience n/a n/a Herbicide tolerance 2015 (to HPPD and glyphosate) Bayer CropScience n/a n/a Herbicide tolerance 2015 (to HPPD and glufosinate) Stein & Rodriguez-Cerezo, JRC, 2009
10 Pipeline characteristics New herbicide resistence, further stacking, some crop composition traits New Herbicide Traits: Dicamba mimics naturally occuring plant hormones (auxins), destroys tissue through uncontrolled cell division and growth DHT Dow AgroSciences Herbicide Traits, confer resistence to 2,4 D and fop herbicides fop herbicides are aryloxyphenoxypropionate fop grass herbicides HPPD inhibitors Hydroxyphenylpyruvate deoxygenase inhibitor, HPPD is important for tyrosine catabolism, tyrosine is important for protein building and plays an important role in photosynthesis ALS inhibitors Acetolactate synthase inhibitors
11 Current Practice of Environmental Risk Assessment
12 The Current Practice of Environmental Risk Assessment Main Shortcomings Focusing on the transgene and not on the GMO as a whole Transgene product testing mainly when pesticidal properties derived from a bacterial production source No tests on equivalence for the bacterial derived product and the GMO produced transgene product No description and integration of relevant aspects of representative receiving environments No adequate exposure analysis No or unsufficient integration of NTOs from
13 The Current Practice of Environmental Risk Assessment Main Shortcomings Pooling of data from field trials over several locations Data from one or maximal 2 vegetation periods only Plot size often not adequate No meaningful statistical evaluation No integration of species of conservation concern Toxicological assessment unsufficient
14 A current example MON x MON Traits and Scope of the Application Traits: Cry 1A Cry 2Ab2 = Cry 3Bb1 + epsps (glyphosate-resistence) = Scope Cultivation of MON x MON Seed production of MON 89034
15 A current example MON Drought tolerance Expresses prokaryotic CspB - cold shock protein Contains npt II Action very unspecific mode of action how drought tolerance is achieved not understood Binds to single stranded RNA influences expression of proteins by stabilizing the mrna for translation several provided studies used test, control or reference material contaminated with MON or maize NK 603
16 Low Level Presence the Dimension But as soon as a GMO is cultivated in a country that is exporting to the EU, even on a small scale, the repercussions can be big: for instance in the case of Herculex, the corresponding GM maize was grown on only one percent of the total maize acreage in the USA, but in approximately two thirds of all samples tested subsequently,traces of Herculex maize were found (Toepfer 2008). Stein & Rodriguez-Cerezo, JRC, 2009
17 Conclusions There is need of improvement of the RA on the conceptual and methodological side Receiving environments and especially protected areas and species of conservation concern need more attention Comparators and comparisons are of growing importance and difficulties Stacked genes and traits need new consideration - concerning i.a. different numbers of stacks and segregation patterns There is growing evidence of contaminated controls and test substances what is the impact on the validity of data in the RA?
18 A future task in good hands Thank you very much for your kind attention! Dr. Beatrix Tappeser Federal Agency for Nature Conservation, Germany Division II 3.3 GMO-Regulation, Biosafety
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