TYPES OF MUSCLE TISSUE STRIATED MUSCLE SKELETAL CARDIAC SMOOTH MUSCLE

Transcription

1 MUSCLE TISSUE

2 TYPES OF MUSCLE TISSUE STRIATED MUSCLE SKELETAL CARDIAC SMOOTH MUSCLE

3 STRIATED SKELETAL MUSCLE TISSUE LONG FIBRES OF MULTINUCLEATED MUSCLE CELLS MUSCLE TISSUE CELL = MUSCLE FIBRE

4 STRIATED SKELETAL MUSCLE TISSUE Longitudinal section: Cylindrical cells, parallel to each other Length up to tens of cm Diameter µm Multinucleated nuclei /cm Nuclei elongated, heterochromatic, peripheral, just beneath sarcolemma Cytoplasm = SARCOPLASM - cross-striations alternating clear and dark bands Vascularized connective tissue between muscle cells

5 STRIATED SKELETAL MUSCLE. HE, OB. 100X longitudinal section Ross HM, Histology: A Text and Atlas, 6 th ed.

6 Striated muscle, Heidenheim stain, ob. 100x

7 STRIATED SKELETAL MUSCLE TISSUE Cross-section: Polygonal/ round sections 1-3 nuclei Nuclei - round, peripheral Cytoplasm contains myofibrils Evenly distributed Grouped Cohnheim fields Vascularized connective tissue between muscle cells

8 STRIATED SKELETAL MUSCLE, CROSS SECTION, COL. HE, OB. 100X

9 MUSCLE ASSOCIATED CONNECTIVE TISSUE ENDOMISSIUM loose connective tissue around each muscle fiber (cell) PERIMISSIUM around several fibers, forming PRIMARY, SECONDARY AND TERTIARY muscle fibers EPIMISSIUM dens irregular connective tissue around the entire muscle Best visible in tri-chrome stains

10 Striated skeletal muscle, cross section ob. 100x Epimissium Endomissium Perimissium Junqueira L et al, Basic Histology: Text & Atlas, 11th ed.

11 MYOTENDINOUS JUNCTION, HE, OB. 100X Ross HM, Histology: A Text and Atlas, 6 th ed. MF muscle fiber;; Tc tenocytes;;

12 ULTRASTRUCTURE of STRIATED MUSCLE MYOFIBRILS Cylindrical fibrils Diameter : 1-2 µm Striated aspect alternation of Light bands I- isotropic In the middle dark Z line Dark bands A- anisotropic In the middle light H band, bisected by dark M line

13 SARCOMERE, TEM

14 STRIATED MUSCLE, TEM M M A, I bands;; ZL Z line;; Sa sarcomere;; SR sarcoplasmic reticulum;; M - mitochondria

15 T Tube SARCOPLASMIC RETICULUM TRIAD T Tube

16 TRIAD STRIATED MUSCLE, TEM

17 STRIATED MUSCLE, TEM M IL Mf Mf M M G IL Mf - myofibrils;; M mitochondrion;; IL lipid droples;; G glycogen

18 STRIATED CARDIAC MUSCLE TISSUE L.M. cardiac muscle fiber Longitudinal section: Length : µm Diameter : 15 µm Nuclei (1, sometimes 2), central, round/oval, euchromatic, one nucleolus Cytoplasm transvers striations Clear perinuclear zone;; may contain LIPOPHUSCIN granules

19 MYOCARDIUM, LONGITUDINALSECTION, HE, OB. 40X

20 MYOCARDIUM Cross-section : Polygonal cells Nuclei: central, rotund, euchromatic Not visible in all sections - Clear perinuclear zone

21 MYOCARDIUM, CROSS-SECTION, HE, OB. 40X Lippincott Williams & Wilkins

22 Purkinje cells MYOCARDIUM, HE, ob. 40x

23 MYOCARDIUM, TEM

24 DIAD SARCOPLASMIC RETICULUM T Tubes

25 MYOCARDIUM, TEM DIAD

26 INTERCALATED DISC Complex junctional structures formed by: a transverse region Zonula adherens Macula adherens ( desmozom) a longitudinal region GAP electric and ionic continuity

27 INTERCALATED DISC

28 SMOOTH MUSCLE TISSUE Longitudinal Section Spindle-shaped cells Length µm Diameter 4-10 µm Nucleus single, centrally located Euchromatic During contraction - twisted nucleus - Cytoplasm eosinophilic, no crossstriations

29 SMOOTH MUSCLE TISSUE Cross-section Round/polygonal cells, various section areas Nucleus - central, rotund, euchromatic, visible only in those sections crossing through the middle of the cell

30 SMOOTH MUSCLE TISSUE 30

31 SMOOTH MUSCLE TISSUE LONGITUDINAL SECTION CROSS SECTION

32 SMOOTH MUSCLE TISSUE, HE, ob 100x

33 SMOOTH MUSCLE TISSUE, cross section, ob 100x

34 SMOOTH MUSCLE TISSUE, TEM Lippincott Williams & Wilkins

35

36 TYPES OF MUSCLE TISSUE

37 SKELETAL STRIATED MUSCLE VS. SMOOTH MUSCLE

38 SMOOTH MUSCLE VS. CARDIAC MUSCLE