Ph.D. student Course Molecular Neurobiology
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1 1 Ph.D. student Course Molecular Neurobiology 3 11 September 2009 Organizers: Joost Verhaagen Netherlands Institute for Neurosciences Meibergdreef BA Amsterdam telephone : (020) fax : (020) j.verhaagen@nin.knaw.nl Guus Smit Dept. of Molecular and Cellular Neurobiology VU University Faculty of Earth and Life Sciences De Boelelaan HV Amsterdam telephone : (020) fax : (020) guus.smit@cncr.vu.nl Graduate School Neurosciences Amsterdam Rotterdam
2 2 List of speakers Netherlands Institute for Neurosciences Koen Bossers Meibergdreef 47 Joost Verhaagen BA Amsterdam Christiaan Levelt Kasper Roet Elly Hol Christian Lohmann Joanna Korecka Arthur Bergen VU University, Dept. of Neurosciences Ronald van Kesteren Faculty of Earth and Life Sciences Marjo van der Knaap De Boelelaan 1087 Guus Smit HV Amsterdam Ka Wan Li Sabine Spijker Pim van Nierop Roel van der Schors Matthijs Verhage Sander Groffen Peter Heutink Ruud Toonen Shushant Jain Rudolf Magnus Institute for Neurosciences Dianne van den Heuvel P.O. Box Peter Burbach TA Utrecht Athena Institute Tjard de Cock Buning Faculty of Earth and Life Sciences falw.vu.nl De Boelelaan HV Amsterdam Netherlands Cancer Institute Titia Sixma Plesmanlaan CX Amsterdam Hubrecht Institute Eugene Berezikov Uppsalalaan CT Utrecht Leiden University Medical Center Erno Vreugdenhil vreugden@lacdr.leidenuniv.nl P.O. Box 9600 Japrien Noordermeer J.N.Noordermeer@lumc.nl RC Leiden Johan den Dunnen ddunnen@humgen.nl Amsterdam Molecular Therapeutics Arie van Oorschot a.vanoorschot@amtbiopharma.com P.O. Box BA Amsterdam Academic Medical Center Frank Baas f.baas@amc.uva.nl P.O. Box DD Amsterdam-Zuidoost
3 3 International Guest Speakers: Swammerdam Lecture Prof.dr. David Ginty, Johns Hopkins University, USA Netherlands Institute for Neuroscience - symposium lecture Prof.dr. Mike Ehlers, Duke University Medical Center, USA Locations Lectures at the VU University (3-8 September): buildings and rooms are indicated in the program. Explanation locations: HG = hoofdgebouw VU = main building VU, De Boelelaan 1105, Amsterdam (e.g. 02A02 = 2nd floor, wing A, room 02.) WN = Wis- en Natuurkunde = science building VUA, De Boelelaan 1085, Amsterdam MF = Medische Faculty = building medical faculty, Van der Boechorststraat 7, Amsterdam (BK = in the basement of the building) For route description, please see: All lectures in the Netherland Institute for Neuroscience (9-11 September) are in the Colloquium Room at the third floor of the Institute at Meibergdreef 47, Amsterdam-Zuidoost. For route description, please see:
4 4 Program Ph.D. course molecular neurobiology Thursday 3 September 2009 (VU) *HG = Main building VU De Boelelaan Welcome and introductory remarks on Guus Smit *HG 02A02 the aim of the program Basics of molecular neuroscience: the gene and gene regulation Overview of molecular neuroscience Guus Smit *HG 02A02 Fundamental aspects concerning the structure of DNA and RNA, the basic organization of a gene, the process of transcription & translation Coffee Manipulation of genes/dna: vectors and their applications Pim van Nierop *HG 02A02 DNA is hosted in vectors. Various types of vectors are available to researchers and their applications will be discussed The polymerase chain reaction and its applications Pim van Nierop *HG 02A Lunch Basics of molecular neuroscience: the genome, what did we learn during the last 10 years? The structure and evolution of the genome: SNPs and CNVs Peter Heutink *HG 02A Tea mirnas: versatile regulators of gene expression Eugene Berezikov *HG 02A02
5 5 Friday 4 September 2009 (VU) *WN = Science buildingvu De Boelelaan 1085 Analysis of DNA and protein protein interactions Sequence analysis Practical introduction to electronic gene databases: what is in it and how to use them? Pim van Nierop *WN F Protein interactions: the yeast-two-hybrid system Guus Smit *WN C Protein crystallography: application in neurobiology Titia Sixma *WN C Lunch Proteomics Introduction to proteomics Ka Wan Li *WN C Tea Application: Mouse models of disease; proteomics analysis of the synapse. Guus Smit *WN C Demonstration: mass spectrometry Roel van der Schors *WN C324
6 6 Monday 7 September 2009 (VU) *MF=Medical Faculty VU Van der Boechorststraat 7 *WN=Science Building VU De Boelelaan 1085 High-throughput gene expression analysis Introduction to micro-array technology Koen Bossers *WN P Application of microarray technology: global assessment of changes in gene expression following peripheral nerve injury Ronald van Kesteren *WN P Coffee LLM3D: Analysis of transcriptional regulation using Ronald van Kesteren computational methods *WN P Lunch Bioinformatics analysis of micro-array datasets: application on human brain expression data sets Gene expression profiling using high-throughput sequencing - measuring all there is?!. Koen Bossers *WN P663 Johan den Dunnen/ Bram t Hoen *WN P Tea Demonstration: microarray analysis Koen Bossers/ Joanna Korecka *MF BK 50
7 7 Tuesday 8 September 2009 (VU) *HG = Main building VU De Boelelaan 1105 High content, medium throughput cellular screening technology Medium-throughput cellular screening of targets identified by Ronald van Kesteren micro-arrays using sirna knock-down technology *HG 12A Application of Cellomics technology: identifying glial targets involved in neuroregeneration Kasper Roet *HG 12A Coffee A cell culture and phenotype screening facility Sushant Jain *HG 12A05 at the VU/VUMC Demonstration of Cellomics KineticScan Reader Ronald van Kesteren/ and cellular phenotype screening facility Sushant Jain Lunch Live cellular imaging of tagged proteins Live imaging of GFP-tagged proteins in neurons Ruud Toonen *HG 12A Imaging structural plasticity and synaptic signaling in intact neuronal circuits Christian Lohmann *HG 12A05
8 8 Wednesday 9 September 2009 (NIN) Genetic modification of the nervous system: in-vivo approaches Viral vector-mediated gene transfer: basic science and therapeutic applications Joost Verhaagen Gene therapy: industrial scale viral vector Arie van Oorschot development for clinical trials Coffee Knock-out mice technology Ruud Toonen Lunch Transgenic mice technology Christiaan Levelt Demonstration: Cold Spring Harbor video of mouse Christiaan Levelt oocyte injections, ES-cell technology, uterus implantation Tea Random mutagenesis, forward genetics/high throughput phenotyping in mice Matthijs Verhage
9 9 Thursday 10 September 2009 (NIN) Application of molecular biology to study basic neurobiological problems Function of Neogenin signalling in neuronal Dianne van den Heuvel development and axon guidance Dopaminergic neurons: transcriptional determinants Peter Burbach Coffee Neurogenesis in the adult brain Erno Vreugdenhil Lunch Axonal pathfinding and synaptogenesis in the fruit fly Japrien Noordermeer Dissecting addiction: the molecular approach Sabine Spijker Tea The law, ethics and genetic modification Tjard de Cock Buning
10 10 Friday 11 September 2009 (NIN) Application of molecular biology to study neurological diseases The Netherlands Brain Bank: high quality human brain tissue to study the molecular neuropathology of brain diseases Elly Hol The molecular biology of Alzheimer s disease Elly Hol Coffee The genetics of developmental disorders of the cerebellum Frank Baas Lunch The genetics and molecular biology of retinal diseases Arthur Bergen Closing symposium of the course Swammerdam Lecture David Ginty - Johns Hopkins University, Baltimore, USA Title: Growth and survival signals controlling the development of the peripheral nervous system Tea NIN-symposium lecture Mike Ehlers - Duke University Medical Center, Durham, USA Title: Membrane and protein dynamics in neuronal plasticity Drinks END OF COURSE
Ph.D. student Course Molecular Neurobiology
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