Ph.D. student Course Molecular Neurobiology
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1 1 Ph.D. student Course Molecular Neurobiology 27 May 4 June 2015 Organizers: Joost Verhaagen Netherlands Institute for Neurosciences Meibergdreef BA Amsterdam telephone : (020) fax : (020) j.verhaagen@nin.knaw.nl Guus Smit Dept. of Molecular and Cellular Neurobiology VU University Faculty of Earth and Life Sciences De Boelelaan HV Amsterdam telephone : (020) fax : (020) guus.smit@cncr.vu.nl Graduate School Neurosciences Amsterdam Rotterdam
2 2 List of speakers Netherlands Institute for Neurosciences Koen Bossers Meibergdreef 47 Joost Verhaagen BA Amsterdam Christiaan Levelt Matthew Mason Christian Lohmann Callan Attwell Inge Huitinga Elizabeth Moloney VU University, Dept. of Neurosciences Ronald van Kesteren Faculty of Earth and Life Sciences Marjo van der Knaap De Boelelaan 1087 Guus Smit HV Amsterdam Ka Wan Li Sabine Spijker Pim van Nierop Matthijs Verhage Sander Groffen Ruud Toonen Danielle Posthuma Vivi Heine Huib Mansvelder University of Amsterdam, UVA Marten Smidt Postbus Arthur Bergen GE Amsterdam Marian Weterman Rudolf Magnus Institute for Neurosciences Giuliano Giuliani P.O. Box Elly Hol TA Utrecht Jeroen Pasterkamp Center for Society and Genetics Martina Cornel Clinical Genetics, VUmc Van der Boechorststraat BT Amsterdam Netherlands Cancer Institute Titia Sixma t.sixma@nki.nl Plesmanlaan CX Amsterdam Leiden University Medical Center Irina Pulyakhina I.Pulyakhina@lumc.nl 2300 RC Leiden Ingrid Verhaart I.E.C.Verhaart@lumc.nl Annemiek Aartsma-Rus International Guest Speakers: Swammerdam Lecture Daniel Geschwind
3 3 Locations: Lectures at the VU University: buildings and rooms are indicated in the program. Explanation locations: WN = Wis- en Natuurkunde = science building VUA, De Boelelaan 1085, Amsterdam For route description, please see: All lectures in the Netherlands Institute for Neuroscience are in the Colloquium Room at the third floor of the Institute at Meibergdreef 47, Amsterdam-Zuidoost. For route description, please see:
4 4 Program Ph.D. course molecular neurobiology Wednesday 27 May 2015 (VU) *WN = Science Building VU De Boelelaan Welcome and introductory remarks on Guus Smit *WN F123 the aim of the program Basics of molecular neuroscience: the gene and gene regulation Overview of molecular neuroscience Guus Smit * WN F123 Fundamental aspects concerning the structure of DNA and RNA, the basic organization of a gene, the process of transcription & translation Coffee Manipulation of genes/dna: vectors and their applications Pim van Nierop * WN F123 DNA is hosted in vectors. Various types of vectors are available to researchers and their applications will be discussed The polymerase chain reaction and its applications Pim van Nierop * WN F Lunch Basics of molecular neuroscience: the genome, what did we learn during the last 10 years? The structure and evolution of the genome: SNPs and CNVs Sander Groffen * WN F Tea Non-coding RNA s: versatile regulators of gene expression Giuliano Giuliani * WN F123
5 5 Thursday 28 May 2015 (VU) *WN = Science buildingvu De Boelelaan 1085 Analysis of DNA and protein protein interactions Sequence analysis Practical: Introduction to electronic gene databases: what is in it and how to use them? Pim van Nierop *WN C Protein interactions: Yeast-two-hybrid system versus mass spectrometry Guus Smit *WN C Protein crystallography: application in neurobiology Titia Sixma *WN C Lunch Proteomics Introduction to proteomics Ka Wan Li *WN C Tea Application: Mouse models of disease; proteomics analysis of the synapse. Ka Wan Li *WN C Demonstration: mass spectrometry Ka Wan Li *WN C147
6 6 Friday 29 May 2015 (NIN) High-throughput gene expression analysis Introduction to micro-array technology Koen Bossers Application of microarray technology: global assessment of changes in gene expression following peripheral nerve injury Ronald van Kesteren Coffee Ronald van Kesteren Lunch Bioinformatics analysis of micro-array datasets: application on human brain expression data sets Koen Bossers Gene expression profiling using high-throughput RNAsequencing - measuring all there is?!. Irina Pulyakhina Tea Demonstration: microarray analysis Koen Bossers/ Matthew Mason
7 7 Monday June (VU) *WN = Science buildingvu De Boelelaan 1085 High content, medium throughput cellular screening technology Cellomics technology: neuronal targets in peripheral nerve injury. Callan Attwell * WN F High-content cellular screening as a discovery tool in neuroscience Ronald van Kesteren * WN F Coffee ips Center: A stem cell culture and phenotype screening facility Vivi Heine at the VU/VUMC *WN F Demonstration of KineticScan and Opera Reader Ronald van Kesteren and cellular phenotype screening facility *WN F Lunch Live cellular imaging of tagged proteins Live imaging of GFP-tagged proteins in neurons Ruud Toonen *WN F Imaging structural plasticity and synaptic signaling in intact neuronal circuits Christian Lohmann * WN F607 Swammerdam Lecture Chairman: Niels Cornelisse Daniel Geschwind *WN Q112 Department of Neurology, Psychiatry and Human Genetics UCLA Los Angeles, CA USA Integrative genomics in Autism Spectrum Disorders Followed by drinks
8 8 Tuesday 2 June 2015 (NIN) Gene delivery to the nervous system: in-vivo approaches Transgenic animal technology Christiaan Levelt Knock-out technology Sander Groffen Coffee Demonstration: Cold Spring Harbor video of mouse Christiaan Levelt oocyte injections, ES-cell technology, uterus implantation Lunch Viral vector technologie and gene therapy Joost Verhaagen Antisense-mediated exon skipping: The example of Ingrid Verhaart/Annemiek Duchenne Muscular Dystrophy Aarstma-Rus Tea Random mutagenesis, forward genetics and high throughput Matthijs Verhage phenotyping in mice
9 9 Wednesday 3 June 2015 (NIN) Application of molecular biology to study basic neurobiological problems Axon guidance molecules: signaling and circuit formation Jeroen Pasterkamp Dopaminergic neurons: transcriptional determinants Marten Smidt Coffee Axon guidance molecules and disease: the example of RGMA Elizabeth Moloney Lunch Optogenetics Huib Mansvelder Dissecting neuropsychiatric disorders: the molecular approach Sabine Spijker Tea Society and Genomics Martina Cornel
10 10 Thursday 4 June 2015 (NIN) Application of molecular biology to study neurological diseases The Netherlands Brain Bank: high quality human brain tissue to study the molecular neuropathology of brain diseases Inge Huitinga The molecular biology of Alzheimer's disease Elly Hol Coffee The genetics and molecular biology of retinal diseases Arthur Bergen Lunch Finding the genetic cause of a neurological disease: Marjan A. Weterman a fishy business? Vanishing white matter diseases Marjo van der Knaap Tea Genome Wide Association studies (GWAS) to decipher Danielle Posthuma Complex brain disorders Drinks - NIN
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